Ability Of Roots Research Articles

PENGARUH KONSENTRASI IBA TERHADAP KEMAMPUAN BERAKAR SETEK PUCUK Alstonia scholaris (L.) R. Br. SEBAGAI UPAYA PENYEDIAAN BIBIT UNTUK REVEGETASI

Alstonia scholaris (L.) R. Br. is an indigenous plant species in Indonesia which has been use for multiple purposes. This plant tolerant to poor nutrient and alkaline soil, therefore it is potential to explore as a revegetation plant. The study was conducted to identify effect of IBA concentrations on rooting ability of A. scholaris shoot cutting. Plant materials (shoot cutting) were collected from trubusan in Mandeh Area, Tarusan, West Sumatra. This study used Completely Randomized Design (CRD). The treatment was IBA concentrations (0 ppm, 50 ppm, 100 ppm, 150 ppm and 200 ppm). The result showed that IBA concentrations was significant different (P ? 0,05) for length, fresh weight and dry weight of root. The application of 150 ppm IBA was the best concentration to enhance the rooting ability of A. scholaris shoot cuttings.

Rhizophora stylosa prop roots even when damaged prevent wood-boring teredinids from toppling the trees

This study examines the ability of live Rhizophora stylosa prop roots to heal damaged tissues and defend against herbivorous attack from teredinids in three mangrove forests. Sites were chosen because damaged roots and teredinid activity were frequent. Responses of 81 R. stylosa roots to three levels of experimental damage were investigated: superficial, moderate and severe. Tannin intensity post damage was analysed using ImageJ. Losses of tissue and numbers of teredinid tunnels within damaged roots among sites were not significant in magnitude. Tissue regrowth varied significantly among roots; moderately damaged roots had an over compensatory regrowth of tissue. Yet, few roots with severe damage demonstrated the same level of excessive tissue regrowth, and many roots lost tissue to necrosis and teredinid attack. Superficially damaged roots did not succumb to teredinid attack. Rhizophora stylosa roots are able to defend against teredinid larval settlement by production of tannins in damaged cortex tissue and by an over compensatory regrowth. This study highlights the resilience and ability of mangroves to heal damaged roots and defend against woodborers. But, when losses of tissues expose the vascular cylinder, teredinid larvae will settle and tunnel into the root. The roots are then open to infection, and cell necrosis.

Nickel accumulation in paddy rice on serpentine soils containing high geogenic nickel contents in Taiwan.

We investigated the extractability of nickel (Ni) in serpentine soils collected from rice paddy fields in eastern Taiwan to evaluate the bioavailability of Ni in the soils as well as for demonstrating the health risks of Ni in rice. Total Ni concentrations in the soils ranged were 70.2-2730mg/kg (mean, 472mg/kg), greatly exceeding the natural background content and soil control standard in Taiwan. Available Ni concentration only accounts for <10% of total soil Ni content; 0.1N HCl-extractable Ni was the more suitable index for Ni bioavailability in the soil to rice than was diethylenetriaminepentaacetic acid (DTPA)-extractable Ni. The accumulation ability of rice roots was much higher than that of its shoots; however, compared with those reported previously, our brown and polished rice samples contained much higher Ni concentrations, within the ranges of 1.50-4.53 and 2.45-5.54mg/kg, respectively. On the basis of the provisional tolerable Ni intake for adults recommended by the World Health Organization (WHO), daily consumption of this rice can result in an excessive Ni intake.

Sedum-dominated green-roofs in a semi-arid region increase CO2 concentrations during the dry season.

Green roofs are expected to absorb and store carbon in plants and soils and thereby reduce the high CO2 concentration levels in big cities. Sedum species, which are succulent perennials, are commonly used in extensive green roofs due to their shallow root system and ability to withstand long water deficiencies. Here we examined CO2 fixation and emission rates for Mediterranean Sedum sediforme on green-roof experimental plots. During late winter to early spring, we monitored CO2 concentrations inside transparent tents placed over 1m2 plots and followed gas exchange at the leaf level using a portable gas-exchange system. We found high rates of CO2 emission at daytime, which is when CO2 concentration in the city is the highest. Both plot- and leaf-scale measurements showed that these CO2 emissions were not fully compensated by the nighttime uptake. We conclude that although carbon sequestration may only be a secondary benefit of green roofs, for improving this ecosystem service, other plant species than Sedum should also be considered for use in green roofs, especially in Mediterranean and other semi-arid climates.

Polymer interaction of the genes SHY2 and MSG1, NPH4 and IAR2 in the inheritance of the Arabidopsis thaliana (L.) Heynh. root system

Roots branching regulation is an important adaptive mechanism for the adaptation of plants to root environments. Elucidation of the genetic mechanisms involved in increase in the degree of plant root branching is essential in improving the responsiveness of crops to supply elements. The aim was to study the interaction of the genes SHY2 and MSG1, NPH4 and IAR2 as attributes of the root system of A. thaliana are inherited. By crossing plants of the mutant lines shy2-2×msg1-2, nph4-1×iar2-1, a segregation in F2 was observed, suggesting an interaction between the polymer SHY2 and MSG1, NPH4 and IAR2 genes. The segregation ratio of the phenotypes in F2 is 15:1. The results presented are of interest for practical use of the economically valuable trait «branching roots» in plant breeding to create varieties and hybrids with the desired properties of mineral nutrition. Our data indicate that the ability of plant roots to increase the degree of branching depends on individual genes and can be inherited through polymer gene interactions. Knowing the polymeric nature of inheritance in the root system, the length of lateral roots in the interaction of genes can be combined by crossing genes and increase the degree of branching of the roots from cultivated plants to create agrochemically effective varieties and hybrids.

Root Activity and Antioxidant Enzyme Activities of Rice Cultivars under Different Iron Toxicity Mitigation Options

Iron (Fe) toxicity is a widespread environmental problem of rice growing area in many parts of the world. Amendments and genotypes can be used to mitigate the Fe toxicity. Several strategies may be adopted by higher plants to cope up with high levels of soluble Fe in their environment. A field experiment was carried out in acidic laterite soil having 400 mg kg−1 di-ethylene tri-amine-penta-acetic acid extractable Fe (DTPA-Fe) to assess the root activity and antioxidant enzyme activities of rice cultivars under different soil management options in Fe toxic soil. The treatments comprised of four cultivars (two each tolerant and susceptible) and six amendments. The amendments resulted into the increase in soil pH as compared to the control, which helps in mitigation of Fe toxicity. Tolerant cultivars recorded significantly higher rice root oxidation and Fe plaque deposition as compared to sensitive cultivars under all the soil management treatments. Cultivars also differed significantly in the amount of Fe plaque on the roots under control. Iron concentration in the roots showed a reverse trend as compared to Fe oxidation and Fe plaque for all the cultivars and soil management treatments. Under high toxic Fe level (control), due to high activity of peroxidase (POD), catalase (CAT) and superoxide dismutase (SOD) mechanisms of antioxidative defense were more active for tolerant cultivars as compared to susceptible cultivars. The effect of defense mechanism of the tolerant cultivars is manifested in yield and under control condition, higher yield were reported for tolerant cultivars as compared to susceptible. Lime application is the most effective way to overcome the Fe toxicity, which increased the yield of both the tolerant and susceptible rice cultivars. Rice roots plays important role in alleviating the Fe toxicity by oxidizing the Fe2+ and excluding its uptake. Iron resistance of the tolerant cultivars is also attributed to the comparatively high levels of POD, CAT, and SOD activities in the leaf tissues. The differential response of the cultivars to the Fe-toxicity is due to differential ability of Fe compartmentation, root oxidation power and ability of cultivars to produce antioxidative stress enzymes. The mechanisms of Fe toxicity tolerance both in plant and soil are result of combined effect of genetics of the crop plant and management interventions.

Injuries of deciduous and permanent teeth in children (diagnostic measures, medical tactics).

A retrospective analysis of 1147 patients’ records from the clinic of the department of surgical dentistry and maxillofacial surgery of childhood of NMU Bogomolets and 944 case histories of patients with traumatic injuries of maxillofacial region in the clinic of the department of pediatric dentistry of SE «Dnepropetrovsk medical academy of Health Ministry of Ukraine» has shown that traumatic damages to teeth make up 18.8% of all injuries in maxillofacial region. It was found that in most cases the cause of injury is fall of a child - 60%, punching on the face - 19%, other causes – 21%. Most often maxillary central incisors are affected - about 70-80%, lateral - 10-20% both in temporary and in shift bite. Lower incisors are injured only in 1-6% of cases. Injuries of the temporary teeth were observed in 30-45% of cases. The aim of the study was to determine the major diagnostic methods and therapeutic measures that have been used in trauma of permanent and temporary teeth in children of different ages. Diagnostic measures included clinical and instrumental studies. Of the additional survey methods of dental trauma in 90% of cases back-side X-ray, in 7-8% – orthopanto­mography, in 1-2% - CT were performed. In dislocations of temporary teeth X-ray examination was carried out only in impacted dislocation. Algorithm of treatment tactics for almost all kinds of temporary teeth dislocations was to remove it, not taking into account the degree of formation of the tooth root and functional ability of the tooth, that involves the development of the indications for their preservation. Most often, for the immobilization of the injured permanent teeth a smooth splin-clamp (85%) was used, it is cheap, easy to manufacture, reliable for fixing, individual as for adaptation, but when applied it leads to injury of periodontal tissues, the development of inflammatory processes in them. Alternative methods of fixation were splint systems, splint-caps and tooth-gum splints. There remains the question of development of non-invasive fixing systems, which would be less traumatized for the tooth and periodontal tissue.

Enhanced Stilbene Production and Excretion in Vitis vinifera cv Pinot Noir Hairy Root Cultures.

Stilbenes are defense molecules produced by grapevine in response to stresses including various elicitors and signal molecules. Together with their prominent role in planta, stilbenes have been the center of much attention in recent decades due to their pharmaceutical properties. With the aim of setting up a cost-effective and high purity production of resveratrol derivatives, hairy root lines were established from Vitis vinifera cv Pinot Noir 40024 to study the organ-specific production of various stilbenes. Biomass increase and stilbene production by roots were monitored during flask experiments. Although there was a constitutive production of stilbenes in roots, an induction of stilbene synthesis by methyl jasmonate (MeJA) after 18 days of growth led to further accumulation of ε-viniferin, δ-viniferin, resveratrol and piceid. The use of 100 µM MeJA after 18 days of culture in the presence of methyl-β-cyclodextrins (MCDs) improved production levels, which reached 1034 µg/g fresh weight (FW) in roots and 165 mg/L in the extracellular medium, corresponding to five-and 570-fold increase in comparison to control. Whereas a low level of stilbene excretion was measured in controls, addition of MeJA induced excretion of up to 37% of total stilbenes. The use of MCDs increased the excretion phenomenon even more, reaching up to 98%. Our results demonstrate the ability of grapevine hairy roots to produce various stilbenes. This production was significantly improved in response to elicitation by methyl jasmonate and/or MCDs. This supports the interest of using hairy roots as a potentially valuable system for producing resveratrol derivatives.

Ability to Remove Na+ and Retain K+ Correlates with Salt Tolerance in Two Maize Inbred Lines Seedlings.

Maize is moderately sensitive to salt stress; therefore, soil salinity is a serious threat to its production worldwide. Here, excellent salt-tolerant maize inbred line TL1317 and extremely salt-sensitive maize inbred line SL1303 were screened to understand the maize response to salt stress and its tolerance mechanisms. Relative water content, membrane stability index, stomatal conductance, chlorophyll content, maximum photochemical efficiency, photochemical efficiency, shoot and root fresh/dry weight, and proline and water soluble sugar content analyses were used to identify that the physiological effects of osmotic stress of salt stress were obvious and manifested at about 3 days after salt stress in maize. Moreover, the ion concentration of two maize inbred lines revealed that the salt-tolerant maize inbred line could maintain low Na+ concentration by accumulating Na+ in old leaves and gradually shedding them to exclude excessive Na+. Furthermore, the K+ uptake and retention abilities of roots were important in maintaining K+ homeostasis for salt tolerance in maize. RNA-seq and qPCR results revealed some Na+/H+ antiporter genes and Ca2+ transport genes were up-regulated faster and higher in TL1317 than those in SL1303. Some K+ transport genes were down-regulated in SL1303 but up-regulated in TL1317. RNA-seq results, along with the phenotype and physiological results, suggested that the salt-tolerant maize inbred line TL1317 possesses more rapidly and effectively responses to remove toxic Na+ ions and maintain K+ under salt stress than the salt-sensitive maize inbred line SL1303. This response should facilitate cell homoeostasis under salt stress and result in salt tolerance in TL1317.

Plant microbiomes and sustainable agriculture: Deciphering the plant microbiome and its role in nutrient supply and plant immunity has great potential to reduce the use of fertilizers and biocides in agriculture.

Bacteria had been around long before multi‐cellular eukaryotes evolved and quickly began to colonize these new life forms as another suitable habitat and potential source of nutrients. Nearly all higher organisms across taxa now have bacterial communities living on and within them—from mutually beneficial symbioses to antagonistic parasitic conflicts. They have co‐evolved with their microbiota and come to depend on it for a range of functions in immunity, metabolism and nutrient absorption. In humans, the microbiota, especially bacteria in the gut, play important roles in all areas, by breaking down indigestible polysaccharides, providing vitamins, mediating nutrient absorption in the gut and helping train the immune system. While research on the human microbiome has stolen the limelight, plant scientists have also been making progress towards elucidating the composition and function of plant microbiomes. Their work has great potential for the future of humanity, as it could help to establish more sustainable agriculture with less dependence on fertilizers, pesticides and herbicides, while increasing yield and nutrient content. Research on the plant microbiome actually predates the animal and human counterparts because microorganisms play an obvious and crucial role in fixing nutrients for the plant, especially nitrogen and phosphorus. Given their vital importance for protein and DNA synthesis and energy production, many plants have evolved close symbiotic relationships with soil bacteria and fungi that supply them with both elements in convenient forms with minimum energy expenditure. Indeed, the close relationships with two specific families of microorganisms set apart the plant microbiome from its animal counterpart: rhizobia nitrogen‐fixing bacteria and mycorrhizal fungi, which greatly enhance the ability of roots to extract various nutrients from the soil. Neither group can survive without the host plant that in return supplies oxygen and products of photosynthesis, mainly proteins and carbohydrates. ### A three‐way relationship The relationship with mycorrhizal fungi seems to be …

Arabidopsis thaliana hairy roots for the production of heterologous proteins

To evaluate the ability of Arabidopsis thaliana hairy roots to produce heterologous proteins, hypocotyls were transformed with Rhizobium rhizogenes harbouring a green fluorescent protein gene (gfp) fused to a plant signal peptide sequence. Hairy root transgenic lines were generated from wild-type or mutant genotypes. A line secreted GFP at 130 mg/l of culture medium. Unlike as was previously found with turnip hairy roots, a His-tag was still attached to approximately 50 % of the protein. Control of the pH and addition of a protease inhibitor to the culture medium resulted in up to 87 % of the GFP retaining the His-tag. A. thaliana hairy roots expressing the human serpina1 (α-1-antitrypsin) gene secreted the protein, which was visible on a PAGE gel. Protein activity in the culture medium was demonstrated using an elastase inhibition assay. A. thaliana hairy roots can now be considered for the production of heterologous proteins, making it possible to mine the numerous genetic resources for enhancing protein production and quality.

The influence of stockplant fertilization on tissue concentrations of nitrogen, carbohydrates and amino acids and on the rooting of leafy stem cuttings of Cola anomala K. Schum (Malvaceae)

Agroforestry tree domestication for forest restoration and fruits and nuts production is ongoing in West and central Africa. It is focused among others on vegetative propagation techniques to capture the superior phenotypes of individual trees. Cola anomala which is a priority target for domestication has been reported amenable to vegetative propagation through rooting of leafy stem cutting, but the rooting efficiency of cuttings collected from non-fertilized stockplants was very poor. The present study addressed the effect of a range of granular NPK 20-10-10 doses (0, 0.5, 1, 2, and 5 g/plant applied at 2-month intervals for 8 months) on growth, total nitrogen content, available carbohydrate content and amino acid content of C. anomala stockplants and the subsequent rooting ability of leafy stem cuttings. Results showed that perseverance fertilizer applications significantly affected growth (p < 0.001), nitrogen content (p < 0.001), carbohydrate content (p = 0.003) and amino acid content (p = 0.001) of stockplant shoots, as well as mortality percentage (p < 0.001), rooting percentage (p = 0.04) and the number of roots (p = 0.02) of subsequently harvested cuttings. The lowest mortality rate (0 %) was associated with the highest rooting percentage (90 ± 7.1 %), while the highest number of roots per rooted cutting (2.61 ± 0.31) was obtained when NPK fertilizer was applied at the rate of 1 g/plant. This dose corresponded to the rate at which stockplant growth was maximized. Of the parameters investigated, only the content of available carbohydrate at the time of excision was significantly correlated with percentage of cuttings rooted (r = 0.90, p = 0.03) and the number of roots per rooted cutting (r = 0.90, p = 0.03). It is concluded that NPK fertilizer applied to stockplants at the rate of 1 g/plant at 2-month intervals for 8 months resulted in the fastest growth and the best rooting ability of subsequently rooted cuttings. This is valuable information to optimize the clonal propagation protocol for C. anomala and other species of the humid tropics of West and Central Africa, especially those considered to be difficult to propagate.

Plastic Responses Mediated by Identity Recognition in Below-Ground Competition in Cycas micronesica K.D. Hill

We studied the ability of Cycas micronesica roots to recognize the identity of competitor roots using pot culture. Pairs of seedlings were grown in single pots for 10.5 months, and morphological traits of the focal plant were determined when the non-focal plant was a half-sibling, a close relative from a mother 170 m away, a distant relative from a habitat 40 km away, or a different species ( Cycas edentata) from Mindanao, Philippines. Most of the measured response traits exhibited similar trends and confirmed that C. micronesica plant roots were able to recognize the identity of competitors. For example, focal plants grown in pots with C. edentata competitors exhibited 66% increase in fine root dry weight, 54% increase in coralloid root dry weight, and 49% increase in total plant dry weight, compared to focal plants grown in competition with half-siblings. Our methods have shown that plot design in ex situ conservation and botanic garden settings would improve by positioning C. micronesica plants adjacent to non-relatives. Recruitment may improve within in situ conservation plots if managers distribute seeds away from the maternal parent so that emerging seedlings are not forced into immediate competition with half-siblings. These findings may be useful for designing more complex experiments to quantify the influence of these plastic root responses on plant nutrient status and physiology.

Developmental Plasticity of Rice Root System Grown under Mild Drought Stress Condition with Shallow Soil Depth; Comparison between Nodal and Lateral roots

:The plasticity in root system development (RSD) is a key trait for the adaptation of rice to mild drought. However, the enhanced RSD due to the plasticity may not be always a sole function of promoted lateral root (LR) production, but also of the integrated responses of nodal root (NR) development. In this study, we aimed to evaluate the effects of mild drought intensities on the development of the NR and LR, and their contribution to the entire RSD. We used six genotypes including KDML105 (indica, lowland adapted), a high lateral rooting ability genotype. The plants were grown up to heading or maturity stage for two years under soil with limited soil depth (20 cm) assuming the presence of the hardpan and at different moisture gradients generated by the line source sprinkler system. The effects of drought intensities generally differed between the development of NR and LR. In both years, all genotypes showed highest LR development under mild drought stress intensities. However, in some genotypes including KDML105, NR development was maintained in a limited soil moisture range only, which was narrower and wetter than that in which LR plasticity was expressed. Furthermore, the entire RSD was maintained only when both the NR and LR were simultaneously promoted or maintained. These results suggest that the NR have less plasticity than the LR in response to drought and the contribution of the plasticity in LR development to the entire RSD is dependent on both the soil moisture and nodal rooting ability.

Soil-binding ability of vegetation roots in enhancing erosion resistance of a shallow slope

Research was undertaken to quantify the root effect of the Johnson grass roots on the resistance increase in resisting surface erosion, in an effort to evaluate soil-binding or anti-erosion ability of the roots. In this study, an innovative means was proposed to evaluate erosion resistance of rooted soils using the traditional slope stability method. The surface erosion was treated as a special slope stability problem on the shallow slip surfaces that are parallel to the slope surface. It was assumed that factors of safety (F.S.) against these shallow slip surfaces are a direct measure of resistance to the surface erosion and the overall slope stability is not our concern. The proposed method to evaluate the surface erosion resistance of rooted soils is simple, straightforward, feasible, and easily implementable. In the research, individual roots in different zones below the slope ground were tested for their tensile strengths, and capabilities to increase shear strength of soil were assessed accordingly. Soil samples with and without roots of the grasses were collected from Ruston, Louisiana. Direct shear tests were conducted to investigate the overall increase in shear strength of the rooted soils. In the slope stability analysis, the anchor reinforcement method (ARM) was developed by considering those individual roots as independent anchor reinforcements. The erosion resistance analyses were performed by utilizing commercial software program Slope/W to evaluate the grass soil-binding capability following the smeared method (SM) and the ARM. Variations in F.S. were investigated for the specific shallow slip surfaces parallel to the slope surface for the plain soil and root-reinforced soil cases.

Superior sinensis tea clones release of GMBS 1, GMBS 2, GMBS 3, GMBS 4, and GMBS 5

Developing of Indonesian green tea with international standards must be supported by availability of exellence sinensis tea plant materials with hight yielding and good quality. Selection mother bush using genetic plant materials of seedling sinensis tea plants at Pasir Sarongge were found 42 exellence mother bushes or 1,35 % from 31.104 bushes collection, but three clones were poor in rooting ability and 11 clones were succeptible to blister blight. We found 28 potential clones with hight yielding, good rooting ability, and resistance to blister blight. Testing material indicated that 15 clones with high yield, good quality, and good taste. Multi-location tests in three years showed that I.2.167; II.1.1; II.1.46; II.2.157; and II.3.109 clones were high in yielding ability and with good stability in performance and broad adaptability. These clones were officialy release by The Evaluation and Variety Release Committee of the Department of Agriculture on its meeting on March 5, 2009 followed by minister decrees of release of these clones as follows: decree number 1979/Kpts/SR.120/4/2009 concern­ing release of GMBS 1 clones, 1980/Kpts/SR.120/4­/2009 concerning release of GMBS 2 clones: 1981/Kpts/SR.120/4/­2009 concerning release of GMBS 3, 1982/Kpts/SR.120/4/ 2009 concerning release of GMBS 4 clones, and 1983/Kpts/SR.120/4/2009 concerning release of GMBS 5.

Deep roots and soil structure.

In this opinion article we examine the relationship between penetrometer resistance and soil depth in the field. Assuming that root growth is inhibited at penetrometer resistances > 2.5 MPa, we conclude that in most circumstances the increases in penetrometer resistance with depth are sufficiently great to confine most deep roots to elongating in existing structural pores. We suggest that deep rooting is more likely related to the interaction between root architecture and soil structure than it is to the ability of a root to deform strong soil. Although the ability of roots to deform strong soil is an important trait, we propose it is more closely related to root exploration of surface layers than deep rooting.

Propagation of &lt;i&gt;Garcinia kola&lt;/i&gt; (Heckel) by stem and root cuttings

The availability of appropriate propagation techniques is a major constraint to the domestication of the forest trees widely used by rural communities; such as (Heckel). This study tested the ability of root and stem cuttings to regenerate vegetatively when treated with indole-3-butyric acid (IBA), and set in non-mist poly-propagator within a shaded nursery. It found that is amenable to propagation by softwood stem cuttings. Attention was given to the effect of cutting age (softwood, semi hardwood) and IBA application with regard to the sprouting and rooting efficiency. Bud and leaf emergence time were also investigated, as was the coppicing ability of the stump. Results revealed that roots and branches from mature forest trees did not sprout under any culture conditions. The highest percentages of rooting (70–85%) were obtained with softwood cuttings set in non-mist poly-propagator, regardless of hormonal treatment. The mean numbers of buds (2.9 ± 0.4) and roots (2.6 ± 0.1) produced by softwood cuttings was significantly greater than those obtained in the Control. The best average emergence time of buds (25.1 days ± 9.3) and leaves (36.8days ± 8.4) was obtained with cuttings treated with IBA and set in non-mist poly-propagator against, 54.4 ± 12.5 and 72.6 ± 3.4 days respectively for the Control. In general, non-mist poly-propagator gave the best propagation results. When coppiced, the shoots emerging from stumps with one node were the most vigorous.Garcinia kola G. kola

Quantification of free auxins in semi-hardwood plant cuttings and microshoots by dispersive liquid–liquid microextraction/microwave derivatization and GC/MS analysis

Several studies have suggested that differences in the natural rooting ability of plant cuttings could be attributed to differences in endogenous auxin levels.

The ability of the DGT soil phosphorus test to predict pasture response in Australian pasture soils – a preliminary assessment

AbstractDiffusive gradients in thin‐films (DGT) technology provides an alternative assessment of available phosphorus (P) for a range of crops, suggesting a preliminary examination of the performance of the new DGT‐P test, compared to existing bicarbonate extractable Olsen and Colwell P tests, for pastures is justified. This study utilized historic data from the Australian National Reactive Phosphate Rock (NRPR) study (1992–1994) that included 25 experimental sites representing a wide range of soil types and climates used for pasture production. Stored (~19 yr) soil samples were analysed for DGT‐P, Olsen P and a single point P buffering index (PBI) and re‐analysed for Colwell P. Results showed the traditional bicarbonate extractable Colwell (r2 = 0.45, P &lt; 0.001) and Olsen P (r2 = 0.27, P &lt; 0.001) methods predicted relative pasture P response more accurately, compared to the novel DGT‐P test (r2 = 0.09, P = 0.03) when all 3 yr of data were examined. We hypothesize that the harsher bicarbonate extraction used for the Olsen and Colwell methods more accurately reflects the ability of perennial pasture roots to access less labile forms of P, in contrast to the DGT‐P test, which does not change the soil pH or dilute the soil and appears unable to fully account for a plants ability to solubilize P. Further studies are needed to compare the capacity of DGT‐P to measure P availability in perennial pasture systems and to better understand the soil chemical differences between pasture and cropping systems.