Root Vegetables Research Articles

Integrated phenotyping of root and shoot growth dynamics in maize reveals specific interaction patterns in inbreds and hybrids and in response to drought.

In recent years, various automated methods for plant phenotyping addressing roots or shoots have been developed and corresponding platforms have been established to meet the diverse requirements of plant research and breeding. However, most platforms are only either able to phenotype shoots or roots of plants but not both simultaneously. This substantially limits the opportunities offered by a joint assessment of the growth and development dynamics of both organ systems, which are highly interdependent. In order to overcome these limitations, a root phenotyping installation was integrated into an existing automated non-invasive high-throughput shoot phenotyping platform. Thus, the amended platform is now capable of conducting high-throughput phenotyping at the whole-plant level, and it was used to assess the vegetative root and shoot growth dynamics of five maize inbred lines and four hybrids thereof, as well as the responses of five inbred lines to progressive drought stress. The results showed that hybrid vigour (heterosis) occurred simultaneously in roots and shoots and was detectable as early as 4 days after transplanting (4 DAT; i.e., 8 days after seed imbibition) for estimated plant height (EPH), total root length (TRL), and total root volume (TRV). On the other hand, growth dynamics responses to progressive drought were different in roots and shoots. While TRV was significantly reduced 10 days after the onset of the water deficit treatment, the estimated shoot biovolume was significantly reduced about 6 days later, and EPH showed a significant decrease even 2 days later (8 days later than TRV) compared with the control treatment. In contrast to TRV, TRL initially increased in the water deficit period and decreased much later (not earlier than 16 days after the start of the water deficit treatment) compared with the well-watered plants. This may indicate an initial response of the plants to water deficit by forming longer but thinner roots before growth was inhibited by the overall water deficit. The magnitude and the dynamics of the responses were genotype-dependent, as well as under the influence of the water consumption, which was related to plant size.

Distribution characteristics of selenium and heavy metal elements in soil-crop system in ankang, shaanxi, china

A total of 229 groups of root vegetables, rice, corn and their root soils were collected in Ankang, Shaanxi, to analyze the characteristics of element migration and transformation in crops and root soil, to compare the migration and enrichment capabilities of various elements in different crops, to explain the characteristics of root soil pH and element correlation, and provide a theoretical basis for agricultural development. Results showed that there were differences in the ability of crops to accumulate various elements in the soil. The absorption capacity of Se, Cd and Zn elements was strong, while the bioaccumulation ability of As, Pb and Cr elements was weak. The contents of Cr, As and Hg were the highest in rice, which were significantly higher than those in root vegetables and corn. The contents of Cd, Cu and Pb in root vegetables were the highest, significantly higher than those in rice and corn. The content of various elements in crops and root soil had a different intensity correlation. Generally speaking, the content of various elements in crops increased with the increase of root soil element content to a certain extent. There was a negative correlation between pH and soil elements, and there was a significant positive correlation between the elements in the root soil of different crops. Bangladesh J. Bot. 52(2): 649-657, 2023 (June) Special

Distribution characteristics of selenium and heavy metal elements in soil-crop system in ankang, shaanxi, china

A total of 229 groups of root vegetables, rice, corn and their root soils were collected in Ankang, Shaanxi, to analyze the characteristics of element migration and transformation in crops and root soil, to compare the migration and enrichment capabilities of various elements in different crops, to explain the characteristics of root soil pH and element correlation, and provide a theoretical basis for agricultural development. Results showed that there were differences in the ability of crops to accumulate various elements in the soil. The absorption capacity of Se, Cd and Zn elements was strong, while the bioaccumulation ability of As, Pb and Cr elements was weak. The contents of Cr, As and Hg were the highest in rice, which were significantly higher than those in root vegetables and corn. The contents of Cd, Cu and Pb in root vegetables were the highest, significantly higher than those in rice and corn. The content of various elements in crops and root soil had a different intensity correlation. Generally speaking, the content of various elements in crops increased with the increase of root soil element content to a certain extent. There was a negative correlation between pH and soil elements, and there was a significant positive correlation between the elements in the root soil of different crops. Bangladesh J. Bot. 52(2): 649-657, 2023 (June) Special

Incorporating carrot pomace-based emulsion to enhance the nutritional value and shelf life of butter

ABSTRACT Carrot pomace, a byproduct of juice extraction from the widely cultivated root vegetable, contains micronutrients and carotenoids that are often discarded as agricultural waste. To address this issue, the present study aimed to create a stable carrot pomace-based emulsion (CPBE) suitable for use in butter production and to evaluate its impact on the physicochemical, microbiological, thermal, oxidative, and fatty acid properties of butter. The CPBE was made with a natural emulsifier and a dispersion medium made of a mixture of vegetable oils in a certain ratio to get the most components, like carotenoids, probiotic culture, and omega-3 fatty acids. The emulsion was selected based on particle size, zeta potential, and color values. Compared to the control sample, the addition of CPBE altered the content of moisture, carbohydrate, protein, and fat, resulting in a higher calorie count. The addition of CPBE also improved the oxidative stability of butter and inhibited the growth of coliforms, Staphylococcus aureus, and psychotropic bacteria during storage, and its biological value increased while its spreadability decreased. Although the color scores were impacted by CPBE, overall acceptability was unaffected. The study shows that the use of CPBE in butter production has potential applications in the functional food industry.

The Phytochemical Screening and Biological Properties of Brassica napus L. var. napobrassica (Rutabaga) Seeds.

Rutabaga, also known as swede and scientifically classified as Brassica napus napobrassica, is a biennial edible root vegetable that belongs to the Brassica genus and is widely cultivated in North Europe and North America. The present study highlights both the phytochemical profile and the in vitro biological properties of rutabaga seed extracts obtained through maceration using solvents of increasing polarity, namely, cyclohexane (CYHA), dichloromethane (DCM), ethyl acetate (EtOAc), methanol (MeOH), and water (H2O). HPLC-DAD was used to identify and quantify phenolic compounds, while volatile compounds were detected using GC-MS. The in vitro antioxidant capacity of the rutabaga seed extracts was evaluated through DPPH free radical scavenging activity. The in vitro anti-inflammatory activity (15-lipoxygenase (15-LOX) enzyme) was determined spectrophotometrically at the same concentration. Additionally, the cytotoxicity of the seed extracts was evaluated against human colon adenocarcinoma cells (Caco-2) and human embryonic kidney cells (HEK-293) using the MTT assay. The rutabaga seed extracts obtained from EtOAc, MeOH, and H2O were particularly rich in reducing sugars, ranging from 189.87 to 473.75 mg/g DW. The MeOH extract displayed the highest concentration of both sugars and polyphenols. Phytochemically, the HPLC-DAD analysis revealed the presence of four phenolic compounds in the tested extracts, including (±) synephrine, gallic acid, p-coumaric acid, and trans-ferulic acid, newly discovered in rutabaga organs. Moreover, a total of ten volatile compounds were identified through GC-MS analysis, both before and after derivatization. At a concentration of 50 µg/mL, the methanol extract exhibited high antioxidant activity with 52.95% inhibition, while CYHA, DCM, and EtOAc exhibited moderate anti-15-LOX activity with less than 30% inhibition. Except for DCM and aqueous extracts, rutabaga seeds did not exhibit any anti-proliferative potential against Caco-2 cell lines. Interestingly, no cytotoxicity was registered for any of the seed extracts against the normal cell line HEK-293. Overall, the obtained data highlight the potential utilization of rutabaga seeds as a source of bioactive compounds in various fields, including pharmaceuticals, nutraceuticals, and functional foods.

Morpho-molecular Characterization of Carrot Soft Rot Incitant, Pectobacterium carotovorum subsp carotovoroum

Carrot is an important root vegetable which plays an important role in human health. Globally, Post harvest diseases are the major constraint in carrot production, especially soft rot which resulting in severe yield loss. Early diagnosis of these post-harvest diseases paves a way for reducing the economic losses. Carrot samples showing typical rotting symptoms were collected from markets of four different districts of Tamil Nadu and the pathogen involved were isolated. Severe carrot soft rot incidence (66.74%) was observed in samples collected from Ooty area of The Nilgiris district and the least disease incidence of (16.21%) was recorded in Perundurai of Erode district. Pathogenicity of soft rot pathogen were conducted and the virulent isolates were identified based on Percent Disease Index (PDI) showing >50% rotting falls under disease grade 9 using disease grade scale (0 to 9). The bacterial isolates (KPB-7 and OCB-5) causing soft rot were characterized using various biochemical assays where in they showed positive response for methyl red, H2S gas production, KOH and catalase tests besides showing negative response for gram’s reaction. Furthermore, molecular characterization of 16s rRNA region revealed the soft rot isolate (KPB-7) as Pectobacterium carotovorum subsp carotovorum (with an accession number OR251119).

Improving the nutritional and livelihood security of landless laborer through the Backyard Farming System

IntroductionIndia is home to around 140 million landless laborers who live below the poverty line and are the most vulnerable group in terms of food and nutritional security. The three critical problems faced by the poor laborer families are poverty, hunger, and malnutrition. To address these problems, a backyard farming system was conceptualized and evaluated with an aim to ensure the nutritional security of landless laborers. The main objective of this work was to utilize the homestead area of 100-150 m2 for ensuring year-round food availability.MethodsIntegration of vegetables, pulses, fruits, spices, fishes, and ducks was done in the available area. Technological interventions with the integration of ducks (Khaki campbell), fish (carps and Self-recruiting species), pulses, and leafy vegetables can help in improving nutrient consumption.Result and discussionA total of 1400 kg of vegetables (including root vegetables and leafy vegetables) can be produced annually from an area of 150 m2 that can fulfil 30-70% of the vitamins (RDA of 70 % of B1 and 30% of B3) and mineral requirements (RDA of 45% of Iron and 30% of Ca) of the average family, as well as providing an annual saving of INR 25,000/annum and an extra income of INR 10,000/annum that can be earned from selling the extra produce. Herbs (Coriander, Mint, and Fenugreek) and spices (Ginger, Turmeric, and Chilli) with antioxidants ranging from 2-13 millimol/100 gm can help in developing a good immune status. Integration with ducks, pond dyke utilization with cucurbits, spices and herbs, and the introduction of self-recruiting species in a composite fish culture system can further enhance the income by INR 14,000/annum after family consumption. Year-round cultivation made the optimum use of the available resources. A net return of INR 30,000 from 300 m2 could be obtained with a B:C ratio of 2.98 as well as generating an employment of 136 mandays. The food produced from the system can contribute to the nutritive requirements throughout the year and lead to a greater diversity in the food consumption pattern of the family.

Anticancer Activity of Mineral-Supplemented Organically Cultivated Carrot on HT-29 Cells and Its Anti-Inflammatory Effect on Mice Splenocytes

Carrot (Daucus carota) is one of the world’s most significant root vegetables, with various bioactivities. This study aimed to investigate the anticancer activity and anti-inflammatory effects of natural dream cultivation carrot (NC). Natural dream cultivation is a cultivation method based on organic farming incorporating minerals. An MTT assay was used to evaluate the inhibitory rate of carrot samples on HT-29 human colon cancer cells, and qPCR was used to assess the mRNA expression of the cell cycle and apoptosis-related genes in the cancer cells. The nitrite oxide (NO) concentration was determined using the Griess method. The levels of inflammatory cytokines in LPS-induced mouse splenocytes were determined using an enzyme-linked immunosorbent assay, and the activity of NK cells was determined using LDH analysis. The results revealed that NC effectively inhibited cancer cell growth rate. Moreover, NC upregulated the mRNA expression of cell-cycle-arrest-related genes (p53 and p21) and apoptosis-related genes (Bim, Bad, Bax, Bak, caspase-9, and caspase-3) in cancer cells while downregulating the expression of anti-apoptotic genes, Bcl-2 and Bcl-xL. NC inhibited NO production and the release of inflammatory cytokines (TNF-α, IL-6, IL-1β, IFN-γ, and IL-12) in LPS-induced mouse splenocytes. NC also demonstrated the ability to stimulate NK cell activation. This study explored the potential mechanisms underlying carrots’ anticancer and anti-inflammatory properties by investigating their inhibitory effects on cancer cells and regulating the inflammatory response. The innovative mineral-supplemented organic cultivation method, as explored in this study, opens new avenues for harnessing the potential of carrots as a functional food source with promising applications in cancer and inflammation management. This research not only provides insights into the bioactive potential of carrots but also contributes to the future development of novel dietary interventions and therapeutics.

Coupling mechanism between vegetation and multi-depth soil moisture in arid–semiarid area: Shift of dominant role from vegetation to soil moisture

Soil moisture (SM) is an essential water source for vegetation, while vegetation also exerts importance on SM during succession. It is crucial to investigate the coupling mechanism between SM and Normalized Difference Vegetation Index (NDVI), which can drive terrestrial carbon sinks while maintaining water sustainability. The Granger causality was used to analyze the unidirectional and bidirectional coupling relationship of SM and NDVI in the 0–289 cm soil layers. The windowed cross correlation was utilized to quantify the bidirectional time lag effect. The findings indicate that the vegetation improved significantly (92.47%) in 2001–2021 in the arid–semiarid area, while the SM in all four layers showed a decreasing trend (>50%). In most areas, NDVI was bidirectional dependent on SM. Vegetation dominated the loss of shallow SM (<100 cm) through root uptake and transpiration, whereas deep SM (>100 cm) in turn dominated the vegetation growth, structure and spatial pattern. At the spatial scale, the top layer of SM in the arid area demonstrated less sensitivity to NDVI due to coupling with the atmosphere. Instead, the influence of NDVI on SM in the semiarid area was dominant, so more attentiveness to the consumption of SM by vegetation is needed. The bidirectional legacy effect could be up to 6 months, attributed to water transport distance, vegetation root systems and physiological mechanisms. The correlation reversed when the lag time was extended or the soil layer was deepened, which means that the greening of vegetation will intensify deep soil drying. Differences in functional traits among plants lead to varying responses. The changes of deep SM need to focus on when implementing artificial meadow construction. This study provides a theoretical basis for optimizing ecological engineering projects, which is essential for restoring global drylands ecosystems.

Study on Cynodon dactylon root system affecting dry–wet cracking behavior and shear strength characteristics of expansive soil

Expansive soil exhibits remarkable characteristics of water absorption expansion and water loss shrinkage, rendering it susceptible to cracking under the alternating dry–wet environments of nature. The generation and development of cracks in expansive soil can result in catastrophic engineering accidents such as landslides. Vegetation protection is an important approach to stabilizing expansive soil slopes and fulfilling ecological protection requirements. In this study, through indoor experiments and theoretical analysis methods, the effects of Cynodon dactylon roots on the crack development and shear strength of expansive soil subjected to dry–wet cycles were analyzed, and the relationship between the crack development and shear strength decay in root-doped expansive soil was explored. Furthermore, the mechanism of vegetative root system action was elucidated. The results show that the Cynodon dactylon root system exerts a significant inhibitory effect on crack development in expansive soil. The crack indexes of root-doped expansive soil exhibit significant phase characteristics during the process of dry–wet cycles. The crack-blocking and reinforcing effect of the root system becomes pronounced as the root-to-soil mass ratio increases and the root diameter decreased. Moreover, the process of crack development in expansive soil is accompanied by a decrease in soil shear strength. The quantitative relationship between crack development and shear strength decay can serve as a basis for predicting the stability of slope soil. Overall, the results highlight the potential of vegetation-based approaches in protecting slopes with expansive soils and have practical implications for ecological protection and engineering design in areas with expansive soils.

An Overview of Biosynthetic Pathway and Therapeutic Potential of Rutin.

Flavonoids are compounds abundantly found in nature and known as a polyphenolic group of compounds having flavancore and show the utmost abundant collection of complexes and are found in fiber-rich root vegetables, fruits, and their eatable items. Due to the presence of hydroxyl groups, flavonoids show various therapeutic activities like antioxidant, antibacterial, antiviral, and antiinflammatory. Substituent groups are responsible for the onset of biochemical actions of flavonoids, which affect the metabolism. The major example of flavonol is rutin, which is constituted of rutinose and quercetin. It is a glycosidic type of flavonoid, similarly identified as purple quercitrin and Vitamin P with over 70 plant species and plant-derived foods, exclusively grapefruit, buckwheat seeds, cherries, apricots, grapes, onions, plums, and oranges. Another name forrutin is a citrus flavonoid known as rutoside, and sophorin. Rutin is a polyphenolic compound having a low molecular weight.

The influence of flour confectionery products based on flour from light-grain rye and processed products of carrots and beets on the behavioral reactions of laboratory rats

Relevance. In modern conditions, due to the increasing popularity of healthy nutrition, more and more attention is being paid to functional foods that can effectively solve the problem of prevention and treatment of various diseases associated with a deficiency of certain substances. In the production of functional food products, natural biologically active substances of plant origin are used, which have an impact not only on health, but also on the behavioral reactions of laboratory animals.Methods. The animals of the control group were kept on a standard diet, consisting of a mixture of cereals in accordance with the recommendations. In the experimental groups, the feed of the main diet was replaced with experimental functional flour confectionery products developed by the authors of the work, and with products sold in retail stores. Behavioral features of laboratory rats were evaluated in the «Open Field». The reliability of the differences was determined by nonparametric statistics (at p &lt; 0.05).Results. The results indicate a positive effect of a diet containing flour confectionery products based on flour from light rye grain with vegetable processing products on the behavioral reactions of laboratory rats. Regular consumption of flour confectionery products based on flour from light-grain rye and processed products of carrot and beet root vegetables in the dining room increases the active-search and reduces the passive-defensive components of the behavior of laboratory animals.

Taro Roots: An Underexploited Root Crop.

Taro (Colocasia esculenta) is a root crop that remains largely underutilized and undervalued despite its abundance and affordability. In comparison to other root vegetables, such as potatoes, yams, carrots, and cassava, taro stands out as a plentiful and low-cost option. As global hunger increases, particularly in Africa, it becomes essential to address food insecurity by maximizing the potential of existing food resources, including taro, and developing improved food products derived from it. Taro possesses a wealth of carbohydrates, dietary fiber, vitamins, and minerals, thereby making it a valuable nutritional source. Additionally, while not a significant protein source, taro exhibits higher protein content than many other root crops. Consequently, utilizing taro to create food products, such as plant-based milk alternatives, frozen desserts, and yogurt substitutes, could play a crucial role in raising awareness and increasing taro production. Unfortunately, taro has been stigmatized in various cultures, which has led to its neglect as a food crop. Therefore, this review aims to highlight the substantial potential of taro as an economical source of dietary energy by exploring the rich fiber, potassium, vitamin C, protein, and other micronutrient content of taro, and providing a foundation for the formulation of novel food products. Furthermore, this paper assesses the nutritional benefits of taro, its current utilization, and its antinutritional properties. It emphasizes the need for further research to explore the various applications of taro and improve on-farm processing conditions for industrial purposes.

RETRACTED: Ding et al. Study on Mechanical Properties of Soil Stabilization by Different Vegetation Roots on High Steep Slope. Sustainability 2023, 15, 2569

The Journal retracts the article entitled “Study on Mechanical Properties of Soil Stabilization by Different Vegetation Roots on High Steep Slope” by Ding et al. [...]

Stability analysis of riverbanks with a dual structure under water-root-soil coupling.

The collapse mechanism of dual-structure vegetation riverbanks at different water levels is unclear. A method for calculating the critical collapse width of a dual-structure vegetation bank under different failure modes that consider the variations in river and groundwater levels and the influence of vegetation roots is proposed. Combined with the influence of flow lateral erosion and slope toe accumulation, a calculation model of riverbank stability was established. The results show that shear failure is the main failure mode when the cohesive soil layer on a dual-structure bank is thick, and the critical collapse width of the bank with root soil is higher than that of the soil bank. The critical collapse width of the bank varied with the water level during different water level periods. Compared with a soil riverbank, a rooted soil riverbank can significantly prolong the bank collapse time. The collapse width of a soil bank without vegetation roots is smaller than that of a rooted soil bank, and the cumulative collapse width is related to calculation time. The greater the thickness of rooted soil, the slower the decay rate of bank stability under water flow erosion.

Sensors for Biomass Monitoring in Vegetated Green Infrastructure: A Review.

Bioretention cells, or rain gardens, can effectively reduce many contaminants in polluted stormwater through phytoremediation and bioremediation. The vegetated soil structure develops bacterial communities both within the soil and around the vegetation roots that play a significant role in the bioremediative process. Prediction of a bioretention cell's performance and efficacy is essential to the design process, operation, and maintenance throughout the design life of the cell. One of the key hurdles to these important issues and, therefore, to appropriate designs, is the lack of effective and inexpensive devices for monitoring and quantitatively assessing this bioremediative process in the field. This research reviews the available technologies for biomass monitoring and assesses their potential for quantifying bioremediative processes in rain gardens. The methods are discussed based on accuracy and calibration requirements, potential for use in situ, in real-time, and for characterizing biofilm formation in media that undergoes large fluctuations in nutrient supply. The methods discussed are microscopical, piezoelectric, fiber-optic, thermometric, and electrochemical. Microscopical methods are precluded from field use but would be essential to the calibration and verification of any field-based sensor. Piezoelectric, fiber-optic, thermometric, and some of the electrochemical-based methods reviewed come with limitations by way of support mechanisms or insufficient detection limits. The impedance-based electrochemical method shows the most promise for applications in rain gardens, and it is supported by microscopical methods for calibration and validation.

Monitoring of foodborne viruses in pre- and post-washed root vegetables in the Republic of Korea

Fresh root vegetables are one of the main causes of viral foodborne diseases; however, the associated risk has not been fully considered. Therefore, the prevalence of five foodborne viruses including norovirus (NoV), hepatitis A virus (HAV), rotavirus (RotaV), adenovirus (AdV), and astrovirus (AstroV) in pre- and post-washed root vegetables (potato, carrot, sweet potato, and radish) were investigated by RT-qPCR or qPCR, nested RT-PCR, and sequencing. AdV and NoV GII were only detected in pre-washed samples. NoV GII was found in 1% and 2% of the potatoes and carrots, respectively and AdV in 4% and 10% of the potatoes and radishes, respectively. HAV was detected in 5.8% and 1.3% of the pre-and post-washed sweet potatoes, respectively and in 8% and 2% of the post-washed carrots and radishes, respectively. NoV GI and AstroV was not detected in any of the root vegetables. Sequencing and phylogenetic analysis revealed that the genotypes of AdV, NoV, and HAV were belonged to human AdV 41, NoV GII.3, and HAV IA and IB, respectively. This molecular surveillance study clearly demonstrated that human pathogenic viruses are found in root vegetables, and it is possible that they may serve as a potential reservoir for such viruses. Therefore, further research is necessary to confirm this hypothesis. Nevertheless, washing produce can lower the risks of contracting diseases due to certain root vegetables.

Ecological Risk and Health Risk of Heavy Metal Pollution in Vegetable Production System of Zhejiang Province

In order to understand the heavy metal contamination of soil and vegetables in the vegetable production system of Zhejiang Province and the health risks of vegetables consumed by residents, typical vegetable production bases in Zhejiang Province were selected as the study areas; 102 pairs of vegetable and soil samples were collected; the distribution characteristics of heavy metals Cd, Cu, Pb, Cr, As, Ni, and Hg in the vegetable production system of Zhejiang Province were analyzed, and the ecological health risks of the vegetable production system were systematically evaluated using the Nemerow composite pollution index, potential ecological risk index, and dietary exposure assessment model. The results showed that Cd in the soil seriously exceeded the standard, with an exceedance rate of 97.2%. The main risk of soil pollution was moderate and mild, and the highest risk was Cd, followed by Pb, Cu, and As. Among vegetables, only a small amount of bean and fruit vegetables exceeded the Cd content, with the exceedance rates of 12.5% and 8.7%, respectively. The BCF of different types of vegetables differed significantly and could be ranked accordingly:leafy vegetables>bean vegetables>melon vegetables>root vegetables. The non-carcinogenic and carcinogenic risks of Zhejiang residents consuming local vegetables were within acceptable limits, with children being more at risk than adults (P<0.01), and Cd and Pb contributing the most to health risks. The overall vegetables produced by the vegetable production system in Zhejiang Province were at a safe level, but there is a need to strengthen the control of Cd and Pb pollution sources.

Accumulation characteristics and evaluation of heavy metals in soils and vegetables of plastic-covered sheds in typical red soil areas of China

The degree of pollution and potential ecological risk of heavy metals (HMs) in the soil of plastic-covered sheds in Jiangxi Province were evaluated by the Nemerow index and potential ecological risk index. The bioconcentration factor and total target hazard quotient (TTHQ) were used to evaluate the enrichment ability and health risk of HMs in vegetables. The mean contents of arsenic (As), cadmium (Cd), chromium (Cr), mercury (Hg) and lead (Pb) in soil were 5.99, 0.373, 17.8, 5.94E-02, and 28.9 mg kg–1, respectively; Cd exceeded the background value and the maximum limit. Most soils had no extremely strong ecological risk. The concentration of Pb in root veg-etables was 0.204 mg kg-1, with the highest concentration of Cd (0.147 mg kg–1) in Ganzhou leafy vegetables. The Hg concentration of leafy vegetables in Jiujiang was 1.41E-02 mg kg–1, which exceeded the maximum limit. The bioconcentration factor of HMs was negatively correlated with pH (P ≤ 0.05). The migration ability of Cd in root vegetables was 1.7-fold to that of leafy vegetables, and was strongest. The TTHQ of all vegetables was less than 1.0, which indicated that there was no significant noncarcinogenic risk in adults. The TTHQ of root vegetables was highest, with Pb in vegetables being major health risk factors. In conclusion, fruit and solanaceous vegetables may be more suitable for planting in plastic-covered shed than root and leafy vegetables; Cd and Pb were identified as the priority control metals under plastic-covered sheds in Jiangxi Province.

Germination and seedling growth of Calluna vulgaris is sensitive to regional climate, heathland succession, and drought

AbstractThe coastal heathlands of Northwest Europe are highly valued cultural landscapes, that are critically endangered due to land use and climatic changes, such as increased frequency and severity of drought events. Our study is the first to assess how the germination and early seedling growth of Calluna vulgaris respond to drought. In a factorial design field experiment, we exposed maternal plants to three in‐situ drought treatments (control, 60%, 90% roof coverage), across three successional stages after fire (pioneer, building, mature), and two regions (60°N, 65°N). Seeds from 540 plants within the experiment were, weighed, and exposed to five water potentials, ranging from −0.25 to −1.7 MPa, in a growth chamber experiment. We recorded germination (percentage, rate), seedling growth (above‐ vs. belowground allocation), and seedling functional traits (specific leaf area [SLA], specific root length [SRL]). Overall variation in germination between regions, successional stages, and maternal drought treatments was largely mediated by variation in seed mass. Plants from the northernmost region had higher seed mass and germination percentages. This is indicative of higher investment in seeds, likely linked to the populations' absence of vegetative root sprouting. Seeds from the mature successional stage germinated to lower final percentages than those from earlier successional stages, especially when the maternal plants had been exposed to drought (60% and 90% roof coverage). Exposure to reduced water availability decreased germination percentage and increased the time to 50% germination. Seedlings fully developed in the range −0.25 to −0.7 MPa, with increased root:shoot and lower SRL during reduced water availability, suggesting a resource‐conservative response to drought during the early stages of development. Our results thus suggest a sensitivity to drought during the germination and seedling life‐history stages that may reduce Calluna's ability to re‐establish from seeds as the incidence and severity of droughts are projected to increase under future climates.