Biomass Allocation Research Articles

Root plasticity improves maize nitrogen use when nitrogen is limiting: an analysis using 3D plant modelling.

Plant phenotypic plasticity plays an important role in nitrogen (N) acquisition and use under nitrogen-limited conditions. However, this role has never been quantified as a function of N availability, leaving it unclear whether plastic responses should be considered as potential targets for selection. A combined modelling and experimentation approach was adopted to quantify the role of plasticity in N uptake and plant yield. Based on a greenhouse experiment we considered plasticity in two maize (Zea mays) traits: root-to-leaf biomass allocation ratio and emergence rate of axial roots. In a simulation experiment we individually enabled or disabled both plastic responses for maize stands grown across six N levels. Both plastic responses contributed to maintaining a higher N uptake, and plant productivity as N availability declined compared with stands in which plastic responses were disabled. We conclude that plastic responses quantified in this study may be a potential target trait in breeding programs for greater N uptake across N levels while it may only be important for the internal use of N under N-limited conditions in maize. Given the complexity of breeding for plastic responses, an a priori model analysis is useful to identify which plastic traits to target for enhanced plant performance.

Mycorrhization enhances plant growth and stabilizes biomass allocation under drought.

Plants and their symbionts, such as arbuscular mycorrhizal (AM) fungi, are increasingly subjected to various environmental stressors due to climate change, including drought. As a response to drought, plants generally allocate more biomass to roots over shoots, thereby facilitating water uptake. However, whether this biomass allocation shift is modulated by AM fungi remains unknown. Based on 5691 paired observations from 154 plant species, we conducted a meta-analysis to evaluate how AM fungi modulate the responses of plant growth and biomass allocation (e.g., root-to-shoot ratio, R/S) to drought. We found that AM fungi attenuate the negative impact of drought on plant growth, including biomass production, photosynthetic performance and resource (e.g. nutrient and water) uptake. Accordingly, drought significantly increased R/S in non-inoculated plants, but not in plants symbiotic with established AM fungal symbioses. These results suggest that AM fungi promote plant growth and stabilize their R/S through facilitating nutrient and water uptake in plants under drought. Our findings highlight the crucial role of AM fungi in enhancing plant resilience to drought by optimizing resource allocation. This knowledge opens avenues for sustainable agricultural practices that leverage symbiotic relationships for climate adaptation.

Respon Pertumbuhan dan Produksi Tanaman Selada Romaine terhadap Otomasi Aerasi pada Sistem Hidroponik Rakit Apung

Romaine lettuce is a nutritious leaf vegetable with a high selling price and its production can be increased using a hydroponic system. Floating raft hydroponics is a hydroponic system with plants floating on styrofoam and their roots submerged in a nutrient solution. Providing aeration using an aerator connected to a timer switch is necessary to increase dissolved oxygen in the nutrient solution to optimize plant growth and production. This research aims to analyze the effect of aeration automation and the best aeration duration on the growth and production of romaine lettuce plants in a floating raft hydroponic system. The research was conducted at Greenhouse UG Technopark in May-June 2023. This experiment used a one-factor Completely Randomized Design with 4 aeration duration treatments: no aeration (control), 6 hours aeration/day, 15 hours/day aeration, and 24 hours/day aeration. Each treatment was repeated 5 times so that there were 20 experimental units and consisted of 5 samples for a total of 100 samples. The results of this research show that aeration 24 hours/day has a real influence on the parameters of plant height in the 3rd week, leaf length, leaf width, leaf wet weight, and root shoot ratio (P<0,05). Aeration 24 hours/day provides the best results for the growth and production of romaine lettuce plants. Aeration automation of 15 hours/day can be used as an alternative from the producer's side to reduce electricity use.

Influence of tree size on the scaling relationships of lamina and petiole traits: A case study using Camptotheca acuminata Decne.

There is a lack of research on whether tree size affects lamina and petiole biomass allocation patterns, whereas the trade-off between leaf biomass allocated to the lamina and the petiole is of significance when considering the hydraulic and mechanical function of the leaf as a whole. Here, Camptotheca acuminata Decne was selected for study because of the availability of trees differing in size growing under the same conditions. A total of 600 leaves for two tree size groups and 300 leaves per group differing in height and trunk diameter were collected. The lamina fresh mass (LFM), lamina dry mass (LDM), lamina area (LA), petiole fresh mass (PFM), and petiole length (PL) of each leaf was measured, and reduced major axis regression protocols were used to determine the scaling relationships among the five functional traits. The bootstrap percentile method was used to determine if the scaling exponents of the traits differed significantly between the two tree size groups. The results indicated that (i) there was a significant difference in the LFM, LDM, PFM, PL, LMA, LFMA and PFM/LFM between large and small trees, but no significant difference in LA; (ii) the LA versus LFM, LA versus LDM, LFM versus PFM, LA versus PFM, and PL versus PFM scaling relationships of the two groups were allometric (i.e., not isometric); (iii) there were significant differences in the scaling exponents of LA versus LFM, LA versus PFM, PL versus PFM between the two groups, but there was no significant difference in the LFM versus PFM scaling relationship between the two groups of trees. The data were also consistent with the phenomenon known as "diminishing returns". These data indicate that tree size influences leaf biomass allocation patterns in ways that can potentially influence overall plant growth, and therefore have an important bearing on life-history strategies.

Belowground plant competition: uncoupling root response strategies of peas.

Belowground plant competition has been shown to induce varying responses, from increases to decreases in root biomass allocation or in directional root placement. Such inconsistencies could result from the fact that root allocation and directional growth were seldom studied together, even though they might represent different strategies. Moreover, variations in belowground responses might be due to different size hierarchies between plants, but this hypothesis has not been studied previously. In a greenhouse rhizobox experiment, we examined the way both root allocation and directional root placement of Pisum sativum are affected by the size and density of Festuca glauca neighbours, and by nutrient distribution. We found that root allocation of P. sativum increased with the density and size of F. glauca. By contrast, directional root placement was unaffected by neighbour size and increased either towards or away from neighbours when nutrients were patchily or uniformly distributed, respectively. These results demonstrate that directional root placement under competition is contingent on the distribution of soil resources. Interestingly, our results suggest that root allocation and directional placement might be uncoupled strategies that simultaneously provide stress tolerance and spatial responsiveness to neighbours, thus highlighting the importance of measuring both when studying belowground plant competition.

Deep tillage combined with straw biochar return increases rice yield by improving nitrogen availability and root distribution in the subsoil

ContextAfter over 20 years of practicing shallow rotary tillage combined with straw direct return in the middle and lower Yangtze regions of China, the yield potential has gradually disappeared, and new alternative tillage practices are required to continuously increase rice yield. ObjectiveThis paper investigates the yield effect and mechanism of deep tillage combined with straw return through two consecutive years of a field experiment. MethodsThree tillage depths (shallow tillage 6–7 cm, ST; medium tillage 12–14 cm, MT; and deep tillage 25–28 cm, DT) plus three straw management methods (no straw return, NR; straw direct return, SR; and straw biochar return based on the same amount of carbon input as SR, BR) were set up as experiment treatments. The aboveground and belowground growth traits of rice and soil nitrogen (N) status were determined. ResultsCompared with ST and MT, the DT enlarged rice root distribution and increased soil N content in the middle and lower soil layers (7–28 cm) and decreased the root–shoot ratio at the flowering stage, which was conducive to absorbing more soil nutrients from the subsoil and allocating more photosynthetic products to the aboveground parts and grain. The MT and DT increased spike number and rice yield compared with ST. Compared with NR, both SR and BR increased soil total and available N contents, and the remaining soil available N content at the harvest stage of BR was lower than that of SR. Straw return (SR and BR) improved rice yield by increasing spike number and kernels per spike, and the effect of BR on increasing rice yield was greater than that of SR. ConclusionsThe DT combined with BR increased root distribution and N content in the subsoil, promoted the utilization of subsoil's nutrients, and increased rice yield. It is therefore a promising tillage practice for achieving high rice yields.

Growth and Yield Responses of Bari Sweet Corn-1 (Zea Mays L.) to Naa at Different Nitrogen Levels

A field experiment was conducted to investigate the effect of foliar application of NAA at varying N-levels on growth and yield responses of BARI Sweet Corn-1 (Zea mays L.). The trial consisted of ten treatments viz. control = without NAA and N fertilizer; RDN (recommended dose of N fertilizer); 25 ppm NAA + RDN; 50 ppm NAA + RDN; 25 ppm NAA + 50% higher RDN; 50 ppm NAA + 50% higher RDN; 25 ppm NAA + 100% higher RDN; 50 ppm NAA + 100% higher RDN; 25 ppm NAA+ 150% higher RDN and 50 ppm NAA + 150% higher RDN. Results showed that application NAA in combination with various nitrogen (N) levels produced better growth than control but with few exceptions. Growth parameters viz. total dry mater (TDM), leaf area (LA), relative growth rate (RGR) and net assimilation rate (NAR) were recorded maximum from 25 ppm NAA in combination with RDN treatment. Findings revealed that 25 ppm NAA in combination with RDN treatment had also produced higher number of leaves per plant, specific leaf area (SLA), biomass duration (BMD) and absolute growth rate (AGR) than RDN treatment although statistically at par to each other. The maximum number of leaves per plant, shoot-root ratio and SLA although recorded maximum from 25 ppm NAA in combination with 100% higher RDN treatment but statistically similar to rest of the combined treatments. Yield attributes and yield of BARI Sweet Corn-1 showed positive response to NAA at varying N-levels with significant variations in majority of cases. The maximum number of cobs and productive cobs per plant, dry weight of cobs, length of cob, number of seed per row and cob, dry weight of tassel, yield per plant and harvest index were also obtained from 25 ppm NAA in combination with RDN treatment. However, maximum number of cobs and productive cobs per plant were also noted from 25 ppm NAA in combination with 100% higher RDN treatment. Significantly maximum yield per plant (114.41g) obtained from 25 ppm NAA in combination with RDN treatment which was 12.02% higher over RDN treatment. Out of ten treatments, foliar application of 25 ppm NAA in combination with RDN treatment produced better stimulation in growth and yield of BARI Sweet Corn-1. Bangladesh J. Bot. 53(2): 337-344, 2024 (June)

Biomass Allocation and Allometric Relationship of Salix gordejevii Branches in Sandy Habitats Heterogeneity in Northern China

The patterns of biomass allocation are crucial for understanding the growth, reproduction, and community functions of plant individuals. We investigated the allometric growth characteristics and biomass allocation patterns of Salix gordejevii fascicular branches in various habitats of the Hunshandake Sandy Land to delve into their adaptability to environmental changes and role in the carbon cycle. We discovered the following: (1) The base diameter-to-branch length of S. gordejevii fascicular branches exhibited allometric growth relationships in mobile dunes and interdune lowlands, whereas it showed isometric growth relationships in semifixed and fixed dunes. As the soil moisture gradient increased, the length growth rate of S. gordejevii fascicular branches became faster than the base diameter growth rate in mobile dunes, demonstrated isometric growth in semifixed and fixed dunes, and was slow in interdune lowlands. (2) The biomasses of S. gordejevii fascicular branches significantly varied across different habitats, with the biomass of each component showing an increasing trend as habitat conditions improved. This study revealed the resource utilization strategies and adaptability of S. gordejevii fascicular branches in different habitats, providing new insights into the carbon sink function of desert ecosystems in semiarid regions.

Belowground plant allocation regulates rice methane emissions from degraded peat soils

Carbon-rich peat soils have been drained and used extensively for agriculture throughout human history, leading to significant losses of their soil carbon. One solution for rewetting degraded peat is wet crop cultivation. Crops such as rice, which can grow in water-saturated conditions, could enable agricultural production to be maintained whilst reducing CO2 and N2O emissions from peat. However, wet rice cultivation can release considerable methane (CH4). Water table and soil management strategies may enhance rice yield and minimize CH4 emissions, but they also influence plant biomass allocation strategies. It remains unclear how water and soil management influences rice allocation strategies and how changing plant allocation and associated traits, particularly belowground, influence CH4-related processes. We examined belowground biomass (BGB), aboveground biomass (AGB), belowground:aboveground ratio (BGB:ABG), and a range of root traits (root length, root diameter, root volume, root area, and specific root length) under different soil and water treatments; and evaluated plant trait linkages to CH4. Rice (Oryza sativa L.) was grown for six months in field mesocosms under high (saturated) or low water table treatments, and in either degraded peat soil or degraded peat covered with mineral soil. We found that BGB and BGB:AGB were lowest in water saturated conditions where mineral soil had been added to the peat, and highest in low-water table peat soils. Furthermore, CH4 and BGB were positively related, with BGB explaining 60% of the variation in CH4 but only under low water table conditions. Our results suggest that a mix of low water table and mineral soil addition could minimize belowground plant allocation in rice, which could further lower CH4 likely because root-derived carbon is a key substrate for methanogenesis. Minimizing root allocation, in conjunction with water and soil management, could be explored as a strategy for lowering CH4 emissions from wet rice cultivation in degraded peatlands.

The Effects of Soil Compaction on the Growth and Architecture of the Seedlings of Species Commonly Used for Afforestation in Iran

The aim of the present study was to elucidate the effects of soil compaction on the seedlings of two species of deciduous (Acer velutinum and Alnus subcordata) and evergreen trees (Pinus eldarica and Pinus nigra) in terms of above- and below-ground morphology in a greenhouse. Six soil compaction levels were applied: the lowest intensity (control), very low, low, moderate, heavy, and very heavy. The results showed that there were different effects according to the species. These effects were on lateral root length, stem diameter, leaf dry biomass, SSL (specific stem length), SRL (specific root length), LMR (leaf mass ratio), RMR (root mass ratio), SMR (stem mass ratio), and R/S (root-to-shoot ratio). The results showed that soil penetration resistance (SPR) had a significant effect on seedling variables such as lateral root length, stem diameter, leaf dry biomass, and SRL (p < 0.05). A. velutinum seedlings have the highest values of growth variables compared to three other species, followed by A. subcordata seedlings. The two evergreen species, Pinus eldarica and Pinus nigra, have the lowest values of these growth variables. It is worth noting that we found that deciduous species had enhanced growth up to a moderate compaction level (1.3 MPa), while the growth decreased at an SPR that was higher than this value.

Interrelationships and Environmental Influences of Photosynthetic Capacity and Hydraulic Conductivity in Desert Species Populus pruinosa

An improved understanding of the mechanisms underlying plant adaptation to habitat heterogeneity can be achieved by clarifying the climate-driving factors of the hydraulic and photosynthetic traits of different populations. With a focus on Populus pruinosa Schrenk, which is the predominant tree species in the desert riparian forests of the Tarim Basin, Xinjiang, this study investigated the hydraulic and photosynthetic trait relationships and their interactions with environmental factors in 11 P. pruinosa populations using a Pearson correlation analysis, plant trait networks, a redundancy analysis, and a least squares linear regression analysis. The results showed that the degree of variation in the hydraulic traits was higher than that in the photosynthetic traits. The net photosynthetic rate (Pn) showed a significantly positive correlation with leaf-specific conductivity (Kl) and the Huber value (Hv). The Hv exhibited a significantly positive correlation with the water-use efficiency and Kl, and the branch–leaf mass ratio significantly affected the hydraulic traits. The groundwater depth (GD) in natural P. pruinosa forest habitats ranged from 3.4 to 7.9 m. With an increase in the annual average temperature, the hydraulic conductivity of the xylem significantly increased; with an increase in GD, Pn and Kl significantly decreased. The temperature annual range, temperature seasonality (standard deviation), min temperature of the coldest month, and GD were significantly correlated with the diameter and average path length of the overall trait network parameters, and these environmental factors affected the coordination of the functional traits of P. pruinosa.

Plant metabolic responses to soil herbicide residues differ under herbivory in two woodland strawberry genotypes

The use of glyphosate-based herbicides (GBHs) to control weeds has increased exponentially in recent decades, and their residues and degradation products have been found in soils across the globe. GBH residues in soil have been shown to affect plant physiology and specialised metabolite biosynthesis, which, in turn, may impact plant resistance to biotic stressors.In a greenhouse study, we investigated the interactive effects between soil GBH residues and herbivory on the performance, phytohormone concentrations, phenolic compound concentrations and volatile organic compound (VOC) emissions of two woodland strawberry (Fragaria vesca) genotypes, which were classified as herbivore resistant and herbivore susceptible. Plants were subjected to herbivory by strawberry leaf beetle (Galerucella tenella) larvae, and to GBH residues by growing in soil collected from a field site with GBH treatments twice a year over the past eight years.Soil GBH residues reduced the belowground biomass of the susceptible genotype and the aboveground biomass of both woodland strawberry genotypes. Herbivory increased the belowground biomass of the resistant genotype and the root–shoot ratio of both genotypes. At the metabolite level, herbivory induced the emission of several VOCs. Jasmonic acid, abscisic acid and auxin concentrations were induced by herbivory, in contrast to salicylic acid, which was only induced by herbivory in combination with soil GBH residues in the resistant genotype. The concentrations of phenolic compounds were higher in the resistant genotype compared to the susceptible genotype and were induced by soil GBH residues in the resistant genotype.Our results indicate that soil GBH residues can differentially affect plant performance, phytohormone concentrations and phenolic compound concentrations under herbivore attack, in a genotype-dependent manner. Soil GBH altered plant responses to herbivory, which may impact plant resistance traits and species interactions. With ongoing agrochemical pollution, we need to consider plant cultivars with better resistance to polluted soils while maintaining plant resilience under challenging environmental conditions.

Biomass allocation and carbon storage in the major cereal crops: A meta‐analysis

AbstractCrop biomass is the reservoir of carbon (C), a valuable input to the soil, thus supporting the soil fauna and enhancing soil health. There are limited studies that compared the major cereal crops for C storage for regenerative agriculture and to optimize C sequestration strategies. The objective of this study was to quantify the extent of variation in biomass allocation and C storage between maize (Zea mays L.), sorghum (Sorghum bicolor [L.] Moench), and wheat (Triticum aestivum L.) for crop production, and C sequestration potential. The study used metadata from 40 global studies that reported on the allocation of plant biomass and C between roots and shoots of the major cereal crops. Key statistics were computed to determine the variability between genotypes for total plant biomass (Pb), shoot biomass (Sb), root biomass (Rb), root‐to‐shoot biomass ratio (Rb/Sb), total plant carbon content, shoot carbon content, root carbon content, total plant carbon stock (PCs), shoot carbon stock, root carbon stock, and root‐to‐shoot carbon stock ratio (RCs/SCs). Maize exhibited the highest variability for Pb (with a coefficient of variation [CV] of 31.2% and a mean of 4.2 ± 1.3 Mg ha−1 year−1), followed by wheat (CV of 24.2% and a mean of 1.5 ± 0.4 Mg ha−1 year−1) and sorghum (CV of 16.8% and a mean of 2.0 ± 0.8 Mg ha−1 year−1), respectively. A similar trend was observed for PCs, with maize (CV of 40.1% and mean of 1.6 ± 0.7 Mg ha−1 year−1) showing the highest total plant C stock variability, followed by wheat (24.4% and 0.2 ± 0.1 Mg ha−1 year−1) and sorghum (16.3% and 0.9 ± 0.3 Mg ha−1 year−1), respectively. Maize (with a CV of 24.4% and mean of 0.1 ± 0.03 Mg ha−1 year−1) exhibited the highest variability for Rb/Sb, while wheat (30.92% and 0.2 ± 0.05 Mg ha−1 year−1) exhibited the highest variability for RCs/SCs. Correlation analysis revealed the following significant associations: Pb and mean annual temperature (MAT) (r = −0.47), and Sb and MAT (r = −0.43), and Pb and mean annual precipitation (MAP) (r = −0.34), and Sb and MAP (r = −0.30). Rb had a strong, significant positive correlation with MAT (r = 0.72) and MAP (r = 0.85). The meta‐analysis revealed that maize and sorghum have the highest variability for Pb and plant carbon stocks, while wheat exhibited the highest variability for the below‐ground biomass and carbon stocks. The data aided in crop selection and suggested that the best cultivars could be developed and identified for production and C sequestration potential for cultivation by farmers, land rehabilitation, and climate change mitigation.

Overstory and understory resource competition variably influences conversion of introduced conifer plantations to native hardwoods

Restoration of native hardwood forests through tree planting may provide significant ecological and economic benefits, but reforestation in natural forests and afforestation on open field sites is challenging. Conversion of existing plantations of introduced conifers to hardwoods may provide an alternative opportunity for restoration. In the Midwest US, large areas of mature, introduced pine (Pinus spp.) plantations exist that have little economic and ecological value. These stands may provide ideal sites for planting of native hardwood species that have similar site preferences to pine. We sought to determine optimal management strategies for converting pine plantations by manipulating overstory canopy density and understory competition. We underplanted American chestnut (Castanea dentata (Marsh.) Borkh.) and northern red oak (Quercus rubra L.) seedlings in three overstory canopy treatments: control, shelterwood, and clearcut. Understory competition was either controlled or not through two growing seasons. After three growing seasons, oak performed best in the clearcut and chestnut in the shelterwood, reflecting variation in the species’ shade tolerance. Chestnut height and root collar diameter (RCD) were double that of oak, and chestnut leaf N content increased with light availability while oak did not, which can be explained by the species’ different adaptive strategies in biomass allocation. Both species had highest photosynthesis in the clearcut. Chestnut seedlings had significantly higher RCD in weeded clearcut and shelterwood plots, and oak in weeded clearcut plots. Weeding in the uncut control plots was ineffective because shade limited competition. Our results indicate that pine plantations offer suitable habitat for these hardwood species and provide insight regarding their growth strategies. Pine shelterwoods and clearcuts are each viable silvicultural conversion options for chestnut and oak if understory competition is controlled.

Anthropogenic and climate impacts on carbon stocks of grassland ecosystems in Inner Mongolia and adjacent region

Up to date, most studies reported that degradation is worsened in the grassland ecosystems of Inner Mongolia and adjacent regions as a result of intensified grazing. This seems to be scientific when considering the total forage or total above-ground biomass as a degradation indicator, but it does not hold true in terms of soil organic carbon density (SOCD). In this study, we quantified the changes of grassland ecosystem carbon stock in Inner Mongolia and adjacent regions from the 1980s to 2000s and identified the major drivers influencing these variations, using the National Grassland Resource Inventory and Soil Survey Dataset in 1980s and the Inventory data during 2002 to 2009 covering 624 sampling plots concerned vegetal traits and edaphic properties across the study region. The result indicated that the above-, below-ground and total vegetation biomass declined from the 1980s to 2000s by ∼ 10 %. However, total forage production increased by 6.72 % when considering livestock intake. SOCD remained stable despite a 67 % increase in grazing intensity. A generalized linear model (GLIM) analysis suggested that an increase in grazing intensity from the 1980s to 2000s could only explain 1.04 % of the total biomass change, while changes in precipitation and temperature explained 17.7 % (p < 0.05) of total vegetation biomass (TVB) change. Meanwhile, SOCD change during 1980s - 2000s could be explained 10.08 % by the soil texture (p < 0.05) and <1.6 % by changes in climate and livestock. This implies that the impacts of climate change on grassland biomass are more significant than those of grazing utilization, and SOCD was resistant to both climate change and intensified grazing. Overall, intensified grazing did not result in significant negative impacts on the grassland carbon stocks in the study region during the 1980s and 2000s. The grassland ecosystems possess a mechanism to adjust their root-shoot ratio, enabling them to maintain resilience against grazing utilization.

Sex-specific strategies of resource utilization and determining mechanisms of Hippophae rhamnoides in response to community succession

Abstract The dioecious plant, Hippophae rhamnoides, is a pioneer species in community succession on the Qinghai-Tibet Plateau (QTP), plays great roles in various ecosystem services. However, the males and females of the species differ both in their morphology and physiology, resulting in a change in the ratio of male to female plants depending on the environment. To further explore the functional traits critical to this sex-based distinctive response in the alpine grassland, we have surveyed the sex ratios, measured their photosynthetic parameters, height, leaf area and biomass allocation. The results showed that (i) The males had higher Pn, light saturation point, apparent quantum efficiency, Amax and lower water-use efficiency (WUE), which exhibited higher utilization efficiency or tolerance to strong light, while the females indicated higher utilization efficiency for low light and water. And it showed sex-specific biomass allocation patterns. (ii) H. rhamnoides populations across the successional stages all showed a male-biased sexual allocation, which was closely related to sex-specific WUE, Pn, root biomass/total biomass and root–crown ratio. (iii) The leaf traits of H. rhamnoides changed from higher Narea, Parea and leaf mass per area in the early and late to lower in the middle, which meant they moved their growth strategy from resource rapid acquisition to conservation as the succession progressed. (iv) The increasing soil total phosphorus mostly contributed to regulating the sex bias of populations and variations of traits during the succession. The results are vital for the management of grassland degradation and restoration due to shrub encroachment on the QTP.

Contrasting light capture strategies between shade-tolerant and -intolerant tree seedlings responding to solar canopy spectral composition

Forest regeneration is a crucial ecological process for promote self-reproduction and restoration, which is essential for maintaining complex community structure and preserving biological resources. Although sunlight have been considered as the main driver of forest regeneration, how light quality shapes functional traits of seedlings is poorly understood in the forest understorey, where spectral compositions of solar radiation change dramatically. We investigated the responses of 15 typical functional traits of Pinus koraiensis and Quercus mongolica to five filter treatments that differed in the spectral transmittance: (a) 95 % of solar radiation was transmitted (280–700 nm); (b) ultraviolet (UV)-B radiation was attenuated (>315 nm); (c) all UV radiation was attenuated (>400 nm); (d) all UV radiation and blue light was attenuated (>500 nm); (e) all UV radiation, blue-green light was attenuated (>580 nm). Our results showed that functional traits responded to UV-B radiation (contrasting treatment [a] vs. [b]) with species-dependence, but consistently showed a positive response to blue light (contrasting treatment [c] vs. [d]). UV-B radiation decreased the relative growth rate (RGR) and leaf area ratio (LAR) of P. koraiensis seedlings by 48 % and 42 %, respectively; however, it increased seedling height, total leaf area (TLA), and above-ground biomass of Q. mongolica. Blue light consistently increased LAR and RGR of two species. Furthermore, Q. mongolica was more plastic in morphological traits (including plant height and TLA) and root/shoot ratio than P. koraiensis to the single spectral region (UV-B, UV-A, blue, and green light), but opposite in physiological and biochemical traits (such as chlorophyll and anthocyanidin). These suggest that two studied species have evolved different light capture strategies in the understorey: Q. mongolica seedlings tended to adjust morphology and biomass allocation to enhance light interception capacity, while P. koraiensis seedlings preferred to adjust physiological and biochemical traits to enhance light utilization efficiency. A better understanding how canopy spectral compositions affect seedling regeneration may provide a new insight for future forest management (i.e. the appropriate gap creation or tree species combination).

Dark septate endophytes enhance the drought tolerance of Haloxylon ammodendron in sterilized and nonsterilized soil

Dark septate endophytes (DSEs) are a type of endophytic fungus that commonly colonize plant root systems in extreme environments, and play a role in enhancing resistance to drought stress. To investigate the potential applications of DSEs in improving drought tolerance of desert plants, three DSE strains isolated from Haloxylon ammodendron for strong drought tolerance - Alternaria tellustris (AT), Cladosporium sp. (CL), and Paraphoma radicina (PR) - were screened through pure culture in vitro. Pot experiments of H. ammodendron were then conducted with different DSE, water, and soil treatments. In both sterilized and nonsterilized soil, DSEs showed growth-promoting and drought-resistant properties, with a stronger effect observed under sterilized soil treatment. The results showed that in sterilized soil, AT and CL increased root biomass, total biomass and root shoot ratio under high drought treatment, while PR effectively enhanced branch number and root biomass under normal water treatment. Physiologically, DSEs improved plant drought tolerance by increasing soluble sugar content and superoxide dismutase activity. Notably, DSE inoculation facilitated the uptake and utilization of soil nutrients such as available phosphorus, nitrate nitrogen, and free amino acids by plants in both sterilized and nonsterilized soil. Overall, our study highlights the potential of DSEs in improving drought resistance and promoting the growth of desert plants.

Effect of Aminating Lignin Loading with Arbuscular Mycorrhizal Fungi on Soil Aggregate Structure Improvement.

Lignin is an important component of plant fiber raw materials, and is a three-dimensional network structure aromatic polymer with abundant resources and a complex structure in nature. Lignin is generally used as industrial waste, and its potential value has not been fully utilized. Modern agriculture extensively uses chemical fertilizers, leading to the gradual degradation of soil fertility and structure, which seriously affects crop growth, nutrient transport, and root respiration function. Based on soil bulk density, porosity, aggregates, and their stability indicators, this study analyzed the effects of aminated industrial lignin and its loading with arbuscular mycorrhizal fungi on soil structure improvement and plant growth. It was hoped that resource-rich lignin could play a beneficial role in improving soil structure and promoting crop growth. The phenolic hydroxyl group of lignin was epoxidized and further aminated to load with arbuscular mycorrhizal fungi. The results indicated that amine-modified lignin could effectively load with arbuscular mycorrhizal fungi. The application of arbuscular mycorrhizal fungi-supported aminated lignin to soil aggregate structure improvement greatly reduced the bulk density of soil, and increased the porosity of soil and the content of large granular soil. Compared with unmodified soil, soil bulk density decreased by 73.08%, the porosity of soil increased by 70.43%, and the content of large granular soil increased by 56.38%. Using the improved soil for corn cultivation efficiently increased the biomass of corn. The plant height was increased by 72.16%, the root-shoot ratio was increased by 156.25%, and other indexes were also improved to varying degrees. The experimental method provides an important basis for the effective utilization of lignin materials in agriculture in the future.

GGE biplot analysis for yield performance and stability assessment of carrot genotypes (Daucus carota L.) under multi-environment trials (METs)

The stability of yield and contributing traits is a very important factor for long-term development and sustainability of cultivars. The genotype x environment (G × E) interaction on the yield stability in twenty-eight genotypes of carrot was studied under eight environments E1-E8 (different sowing dates) over the two years (2019–20 and 2020–21) at Vegetable Research Farm, Punjab Agricultural University, Ludhiana. The experiment was laid out in randomized complete block design with three replications under each environment. At each harvest, data was recorded for root yield (q acre−1), marketable yield (q acre−1), root length (cm), root weight (g), root-shoot ratio, root diameter (cm), core diameter (cm), shoot length (cm), shoot weight (g) and number of leaves plant−1. The data was analyzed with ANOVA, mixed models, BLUPs, GGE biplot and multi stability index for stability statistics. For all the traits, pooled analysis of variance revealed highly significant (p < 0.01) variations between genotypes, environments and genotype by environment (G × E interaction). The mean vs. stability view of GGE biplots revealed that genotype PC-171 (G6) performed better in the months of August and September of both the years (E1, E2, E5 and E6). PC-160 (G3) followed by Cartvar-2 (G25) performed better in the month of October (E3 and E7) for the root yield parameter. Mean across the environments and BLUP values of genotypes for all the traits showed similar results. Hence, GGE biplot graphically explained the interrelationships between the tested environments and genotypes, classified genotypes into three categories early, mid or late season based on their mean performance and stability in the given growing environment.