Root Resistance Research Articles

Evolutionary analysis of MADS-box genes in buckwheat species and functional study of FdMADS28 in flavonoid metabolism

The MADS-box gene family is a transcription factor family that is widely expressed in plants. It controls secondary metabolic processes in plants and encourages the development of tissues like roots and flowers. However, the phylogenetic analysis and evolutionary model of MADS-box genes in Fagopyrum species has not been reported yet. This study identified the MADS-box genes of three buckwheat species at the whole genome level, and conducted systematic evolution and physicochemical analysis. The results showed that these genes can be divided into four subfamilies, with fragment duplication being the main way for the gene family expansion. During the domestication process from golden buckwheat to tartary buckwheat and the common buckwheat, the Ka/Ks ratio indicated that most members of the family experienced strong purification selection pressure, and with individual gene pairs experiencing positive selection. In addition, we combined the expression profile data of the MADS genes, mGWAS data, and WGCNA data to mine genes FdMADS28/48/50 that may be related to flavonoid metabolism. The results also showed that overexpression of FdMADS28 could increase rutin content by decreasing Kaempferol pathway content in hairy roots, and increase the resistance and growth of hairy roots to PEG and NaCl. This study systematically analyzed the evolutionary relationship of MADS-box genes in the buckwheat species, and elaborated on the expression patterns of MADS genes in different tissues under biotic and abiotic stresses, laying an important theoretical foundation for further elucidating their role in flavonoid metabolism.

Hollow Mesoporous Silica Nanoparticles as a New Nanoscale Resistance Inducer for Fusarium Wilt Control: Size Effects and Mechanism of Action.

In agriculture, soil-borne fungal pathogens, especially Fusarium oxysporum strains, are posing a serious threat to efforts to achieve global food security. In the search for safer agrochemicals, silica nanoparticles (SiO2NPs) have recently been proposed as a new tool to alleviate pathogen damage including Fusarium wilt. Hollow mesoporous silica nanoparticles (HMSNs), a unique class of SiO2NPs, have been widely accepted as desirable carriers for pesticides. However, their roles in enhancing disease resistance in plants and the specific mechanism remain unknown. In this study, three sizes of HMSNs (19, 96, and 406 nm as HMSNs-19, HMSNs-96, and HMSNs-406, respectively) were synthesized and characterized to determine their effects on seed germination, seedling growth, and Fusarium oxysporum f. sp. phaseoli (FOP) suppression. The three HMSNs exhibited no side effects on cowpea seed germination and seedling growth at concentrations ranging from 100 to 1500 mg/L. The inhibitory effects of the three HMSNs on FOP mycelial growth were very weak, showing inhibition ratios of less than 20% even at 2000 mg/L. Foliar application of HMSNs, however, was demonstrated to reduce the FOP severity in cowpea roots in a size- and concentration-dependent manner. The three HMSNs at a low concentration of 100 mg/L, as well as HMSNs-19 at a high concentration of 1000 mg/L, were observed to have little effect on alleviating the disease incidence. HMSNs-406 were most effective at a concentration of 1000 mg/L, showing an up to 40.00% decline in the disease severity with significant growth-promoting effects on cowpea plants. Moreover, foliar application of HMSNs-406 (1000 mg/L) increased the salicylic acid (SA) content in cowpea roots by 4.3-fold, as well as the expression levels of SA marker genes of PR-1 (by 1.97-fold) and PR-5 (by 9.38-fold), and its receptor gene of NPR-1 (by 1.62-fold), as compared with the FOP infected control plants. Meanwhile, another resistance-related gene of PAL was also upregulated by 8.54-fold. Three defense-responsive enzymes of POD, PAL, and PPO were also involved in the HMSNs-enhanced disease resistance in cowpea roots, with varying degrees of reduction in activity. These results provide substantial evidence that HMSNs exert their Fusarium wilt suppression in cowpea plants by activating SA-dependent SAR (systemic acquired resistance) responses rather than directly suppressing FOP growth. Overall, for the first time, our results indicate a new role of HMSNs as a potent resistance inducer to serve as a low-cost, highly efficient, safe and sustainable alternative for plant disease protection.

Fracture Strength of Tooth Roots Following Canal Preparation by Three Rotary File Systems: An In Vitro Study.

Since the beginning of modern endodontics, there have been many concepts, strategies, and techniques for root canal preparation. A mind-boggling variety of files have developed for negotiating and shaping them throughout the years. Today's most secure, most effective, and simplest file system combines the most reliable design elements of the past with the latest technological advances to create the most effective file system. So, the need for the study is to evaluate the fracture strength of tooth roots following canal preparation by three rotary file systems: ProTaper Universal file system (Dentsply, USA), ProTaper Next file system (Dentsply Sirona USA), and Neolix A1 nickel-titanium (NiTi) file system (Orikam Healthcare India Pvt Ltd., New Delhi, India). Ninety human mandibular molars were selected for the study. Inclusion criteria include human mandibular first and second molars and teeth removed for routine clinical reasons, and intact apices were selected, excluding cases with root surface caries, root surface fissures, teeth with immature root apex, mesial canal fusion, extremely short roots, thin roots, or curved roots. All teeth were preserved in a solution of 10% neutral buffered formalin for two weeks and then transferred to distilled water for examination. The teeth were randomly divided into three groups. Access cavities were created, and working lengths were determined. Groups 1, 2, and 3 underwent shaping using ProTaper Universal, ProTaper Next, and Neolix A1 (NiTi) file systems, respectively, following guidelines. Canals were irrigated with sodium hypochlorite and ethylenediaminetetraacetic acid (EDTA) and were obturated up to the mid-root region with AH Plus sealer. To facilitate fracture testing, obturation was performed to distribute the load from the spreader to the canal wall. The EndoSequence and Quick-Fill obturation system were utilized to fill the apical half of the canal with gutta-percha material. After obturation, the distal root of each tooth was cut, while the mesial root was securely positioned in a putty material. A universal testing machine was employed for the fracture tests, operating at a cross-head speed of 1 mm/min. The machine was equipped with a D11 hand spreader tip, which was inserted into the root canal to make contact with the gutta-percha. Gradual force was applied to the root canal until a fracture occurred, at which point the force application was stopped. The amount of force required to cause the fracture was measured in newtons. Data were collected and recorded using IBM SPSS Statistics for Windows, Version 17.0 (Released 2008; IBM Corp., Armonk, New York, United States) and then transferred to Microsoft Excel for analysis. Descriptive statistics, mean, and standard deviation were used for continuous data. The fracture resistance of dental roots treated with three types of files was compared using a one-way ANOVA. Graphs were generated using Excel and Word. A significance level of p<0.01 was chosen. ANOVA indicated significant differences in mean fracture resistance: Neolix A1 (NiTi) (95.3 N) > NEXT (91.0 N) > universal (86.6 N), with a p-value of 0.004 (<0.001), confirming statistical significance. The study concludes that the canal instrumented with Neolix A1 (NiTi) exhibits higher fracture resistance after canal instrumentation compared to ProTaper Next and ProTaper Universal.

Study on the characteristics and influence factors of pull-out resistance of vetiver root in expansive soil

Study on the characteristics and influence factors of pull-out resistance of vetiver root in expansive soil

Plant mucilage increases pull‐out resistance of root analogues from soil

AbstractThe interface between plants' roots and soil is strongly affected by rhizodeposits, especially mucilage, that change mechanical and hydrological behaviour. In addition to impacts to aggregation, water capture and root penetration, rhizodeposits may also affect the pull‐out resistance of plant roots. Due to the complex architecture of plant roots and an inability to restrict rhizodeposit production, this study used a simplified system of wooden skewers to simulate roots and chia seed mucilage as a model to simulate rhizodeposit compounds. Pull‐out tests were then carried out to measure the impacts of mucilage, and one (WD1) or two (WD2) cycles of wetting and drying of soils. Using a mechanical test frame, the maximum pull‐out resistance (Fmax) and pull‐out displacement (dL) were recorded, allowing for pull‐out energy (E), average pull‐out force () and bond strength (τmax) to be calculated. The results showed that all pull‐out parameters of the samples with added rhizodeposit compounds tended to decrease between WD1 and WD2, but they were still significantly greater than without the added mucilage. The model rhizodeposit increased all pull‐out parameters by a minimum of 30%. With an additional wet–dry cycle, the mucilage tended to cause a decline in pull‐out parameters relative to a single wet‐dry cycle. This suggests mucilages could enhance the mechanical resistance of roots to pull‐out, but resistance decreases over time with cycles of wetting and drying. To conclude, an important role of mucilage is pull‐out resistance, which has relevance to plant anchorage and root reinforcement of soils.

Molecular mechanism of drought resistance in soybean roots revealed using physiological and multi-omics analyses

Soybeans are one of the most cultivated crops worldwide and drought can seriously affect their growth and development. Many studies have elucidated the mechanisms through which soybean leaves respond to drought; however, little is known about these mechanisms in roots. We used two soybean varieties with different drought tolerances to study the morphological, physiological, and molecular response mechanisms of the root system to drought stress in seedlings. We found that drought stress led to a significant decrease in the root traits and an increase in antioxidant enzyme activity in the two varieties. Drought-resistant varieties accumulate large amounts of flavonoids and phenolic acids at the metabolic level, which causes variations in drought resistance. Additionally, differences in gene expression and drought-resistance pathways between the two varieties were clarified using transcriptome analysis. Through a multi-omics joint analysis, phenylpropanoid and isoflavonoid biosynthesis were identified as the core drought resistance pathways in soybean roots. Candidate genes and marker metabolites affecting drought resistance were identified. The phenylpropanoid pathway confers drought tolerance to roots by maintaining a high level of POD activity and mediates the biosynthesis of various secondary drought-resistant metabolites to resist drought stress. This study provides useful data for investigating plant root drought responses and offers theoretical support for plant breeding for drought resistance.

Biocontrol Efficacy and Induced Resistance of Paenibacillus polymyxa J2-4 Against Meloidogyne incognita Infection in Cucumber.

Meloidogyne incognita is one of the most destructive agricultural pathogens around the world, resulting in severe damage to yield and quality in agricultural production. Biological control promises to be a great potential alternative to chemical agents against M. incognita. Paenibacillus polymyxa J2-4, isolated from ginger plants injured by M. incognita, has shown excellent biocontrol efficacy against M. incognita in cucumber. In vitro experiments with the strain J2-4 resulted in a correct mortality rate of 88.79% (24 h) and 98.57% (48 h) for second-stage juveniles (J2s) of M.incognita. Strain J2-4 significantly suppressed nematode infection on potted plants, with a 65.94% reduction in galls and a 51.64% reduction in eggs compared with the control. The split-root assay demonstrated that strain J2-4 not only reduced J2s' invasion but also inhibited nematode development through the dependence on salicylic acid and jasmonic acid signaling of strain J2-4 induction of plant resistance in local and systemic roots of cucumbers. Genomic analysis of strain J2-4 indicated biosynthetic gene clusters encoding polymyxin, fusaricidin B, paenilan, and tridecaptin. In addition, genetic analysis showed that none of the genes encoding virulence factors were detected in the genome of J2-4 compared with the pathogenic Bacillus species. Taking all the data together, we conclude that P. polymyxa J2-4 has potential as a biological control agent against M. incognita on cucumbers and can be considered biologically safe when used in agriculture.

A biostimulant yeast, Hanseniaspora opuntiae, modifies Arabidopsis thaliana root architecture and improves the plant defense response against Botrytis cinerea

Main conclusionThe biostimulant Hanseniaspora opuntiae regulates Arabidopsis thaliana root development and resistance to Botrytis cinerea.Beneficial microbes can increase plant nutrient accessibility and uptake, promote abiotic stress tolerance, and enhance disease resistance, while pathogenic microorganisms cause plant disease, affecting cellular homeostasis and leading to cell death in the most critical cases. Commonly, plants use specialized pattern recognition receptors to perceive beneficial or pathogen microorganisms. Although bacteria have been the most studied plant-associated beneficial microbes, the analysis of yeasts is receiving less attention. This study assessed the role of Hanseniaspora opuntiae, a fermentative yeast isolated from cacao musts, during Arabidopsis thaliana growth, development, and defense response to fungal pathogens. We evaluated the A. thaliana–H. opuntiae interaction using direct and indirect in vitro systems. Arabidopsis growth was significantly increased seven days post-inoculation with H. opuntiae during indirect interaction. Moreover, we observed that H. opuntiae cells had a strong auxin-like effect in A. thaliana root development during in vitro interaction. We show that 3-methyl-1-butanol and ethanol are the main volatile compounds produced by H. opuntiae. Subsequently, it was determined that A. thaliana plants inoculated with H. opuntiae have a long-lasting and systemic effect against Botrytis cinerea infection, but independently of auxin, ethylene, salicylic acid, or jasmonic acid pathways. Our results demonstrate that H. opuntiae is an important biostimulant that acts by regulating plant development and pathogen resistance through different hormone-related responses.

Bacterial indole-3-acetic acid: A key regulator for plant growth, plant-microbe interactions, and agricultural adaptive resilience

Indole-3-acetic acid (IAA), a fundamental phytohormone categorized under auxins, not only influences plant growth and development but also plays a critical role in plant-microbe interactions. This study reviews the role of IAA in bacteria-plant communication, with a focus on its biosynthesis, regulation, and the subsequent effects on host plants. Bacteria synthesize IAA through multiple pathways, which include the indole-3-acetamide (IAM), indole-3-pyruvic acid (IPyA), and several other routes, whose full mechanisms remain to be fully elucidated. The production of bacterial IAA affects root architecture, nutrient uptake, and resistance to various abiotic stresses such as drought, salinity, and heavy metal toxicity, enhancing plant resilience and thus offering promising routes to sustainable agriculture. Bacterial IAA synthesis is regulated through complex gene networks responsive to environmental cues, impacting plant hormonal balances and symbiotic relationships. Pathogenic bacteria have adapted mechanisms to manipulate the host’s IAA dynamics, influencing disease outcomes. On the other hand, beneficial bacteria utilize IAA to promote plant growth and mitigate abiotic stresses, thereby enhancing nutrient use efficiency and reducing dependency on chemical fertilizers. Advancements in analytical methods, such as liquid chromatography–tandem mass spectrometry, have improved the quantification of bacterial IAA, enabling accurate measurement and analysis. Future research focusing on molecular interactions between IAA-producing bacteria and host plants could facilitate the development of biotechnological applications that integrate beneficial bacteria to improve crop performance, which is essential for addressing the challenges posed by climate change and ensuring global food security. This integration of bacterial IAA producers into agricultural practice promises to revolutionize crop management strategies by enhancing growth, fostering resilience, and reducing environmental impact.

The N-terminal α2 helix element is critical for the activity of the rice transcription factor MYC2.

Jasmonates (JAs) are a class of phytohormones that play a crucial role in plant growth, development, and environmental stress responses. Central to JA signaling are the MYC2-type transcription factors, as they activate the expression of JA-responsive genes. We previously used CRISPR-Cas9-based genome editing to engineer rice OsMYC2 and yielded a mutant (myc2-5) with a single amino acid (aa) deletion (75I) outside the known functional domains of the protein. This myc2-5 mutant also showed some JA-deficient phenotypes, promoting us to investigate how 75I deletion affects JA responses. The mutation is found in the α2 helix element at the N-terminal of OsMYC2. The deletion of 75I in OsMYC2 rendered plants deficient in most of the JA responses, including root growth, leaf senescence, spikelet development, and resistance to pathogens and herbivores. Biochemical assays revealed that the 75I deletion markedly reduced OsMYC2 protein accumulation, subsequently diminishing its transcriptional activity. However, the deletion did not influence the protein's subcellular localization, DNA-binding capability, or its interactions with JAZ transcriptional repressors and the Mediator complex subunit MED25. Additionally, the screening of seven other deletions in the α2 helix further reinforces the importance of this protein element. Our results highlight the significance of the α2 helix in the N-terminus for OsMYC2's functionality, primarily through modulating its protein levels. This insight expands our knowledge of JA signaling and opens new avenues for research into the yet-to-be-explored domains of the MYC2 protein, with the potential to tailor JA responses in rice and other plant species.

Assessment of fracture resistance of mesial roots of mandibular molars prepared by NiTi rotary files of different tapers and alloys (A Comparative in-vitro study)

Assessment of fracture resistance of mesial roots of mandibular molars prepared by NiTi rotary files of different tapers and alloys (A Comparative in-vitro study)

Effect of crosslinking/antioxidant agents as final irrigant on the fracture resistance of endodontically treated root after radiotherapy.

To investigate the effects of Epigallocatechin gallate (EGCG) and curcumin, as a final irrigant on the fracture resistance of irradiated root that obturated with an epoxy resin sealer. Eighty mandibular premolars were randomly divided into non-irradiated (NIR) and irradiated (IR) groups. The teeth were irradiated at 2 Gy per fraction, 5 times a week for a total dose of 60 Gy over 6 weeks. All specimens were decoronated, remaining 13±1 mm root length. Two groups were subdivided into four groups (n = 10): 1) non-instrumented; the intact root served as control. The other roots were instrumented with a pro-taper NiTi rotary system. The final irrigation used was 17% EDTA, followed by three irrigation solution groups; 2) 2.5% NaOCl, 3) 0.02% EGCG, and 4) 0.1% curcumin. Root canals were filled with gutta-percha and AH plus. All specimens were embedded in self-curing acrylic resin and loaded vertically at 1 mm/min until fracture occurred. Also, sealer penetration was assessed by confocal laser scanning microscopy (CLSM). The data were evaluated statistically using two-way ANOVA and Tukey test (α = 0.05). In irradiated roots, fracture resistance of EGCG and curcumin groups did not differ from non-instrumented roots, but they were higher than the NaOCl group (P = 0.006). However, NaOCl, EGCG, and curcumin in irradiated roots had comparable strength that was higher than in the non-instrumented group (p<0.001). Difference between irradiated and non-irradiated roots was observed only for NaOCl and non-instrumented groups (P≤0.004). In irradiated roots, a higher sealer penetration was observed in EGCG and curcumin groups compared to NaOCl. EGCG and curcumin could be promising final irrigants to reverse the adverse effect of radiotherapy on the strength of irradiated roots obturated with AH Plus sealer.

Genomic blueprints of soybean (Glycine max) pathogen resistance: revealing the key genes for sustainable agriculture.

Soybean (Glycine max ) is an important oilseed, protein and biodiesel crop. It faces significant threats from bacterial, fungal and viral pathogens, which cause economic losses and jeopardises global food security. In this article, we explore the relationship between soybeans and these pathogens, focusing on the molecular responses that are crucial for soybeans defence mechanisms. Molecular responses involve small RNAs and specific genes, including resistance (R) genes that are pivotal in triggering immune responses. Functional genomics, which makes use of cutting-edge technologies, such as CRISPR Cas9 gene editing, allows us to identify genes that provide insights into the defence mechanisms of soybeans with the focus on using genomics to understand the mechanisms involved in host pathogen interactions and ultimately improve the resilience of soybeans. Genes like GmKR3 and GmVQ58 have demonstrated resistance against soybean mosaic virus and common cutworm, respectively. Genetic studies have identified quantitative trait loci (QTLs) including those linked with soybean cyst nematode, root-knot nematode and Phytophthora root and stem rot resistance. Additionally, resistance against Asian soybean rust and soybean cyst nematode involves specific genes and their variations in terms of different copy numbers. To address the challenges posed by evolving pathogens and meet the demands of a growing population, accelerated soybean breeding efforts leveraging functional genomics are imperative. Targeted breeding strategies based on a deeper understanding of soybean gene function and regulation will enhance disease resistance, ensuring sustainable agriculture and global food security. Collaborative research and continued technological advancements are crucial for securing a resilient and productive agricultural future.

Comparative Evaluation of Fracture Resistance of Bioceramics as Obturating Material with and without Guttapercha

Background and Aim Bioceramic sealers have revolutionized endodontic practice. However their use as obturating materials has not yet been evaluated. Therefore the present study aimed to evaluate the fracture resistance of root filled teeth obturated with bioceramic-based sealer BioRoot RCS epoxy resin AH plus and Zinc oxide eugenol ZOE based sealers with and without guttapercha using various obturation techniques.Methods Ninety human single-rooted mandibular premolar teeth were extracted decoronated and standardized to a root length of 13 mm. The teeth were instrumented using the Neoendo flex rotary system up to an apical size of 25. Subsequently the specimens were divided into three groups n30 based on the obturation materials and techniques employed. Group 1 Lateral condensation with 0.02 guttapercha LC Group 2 Single cone obturation with 0.06 gutta-percha SC Group 3 Complete sealer obturation without gutta-percha CS. Each experimental group was divided into three sub-groups n10 using following sealersA ZOE B AH plus C BioRoot RCS. The specimens were coronally sealed with Cavit mounted in an acrylic mould and fracture resistance was determined using Universal testing machine and the obtained values were tabulated. Statistical analysis was done using One-way ANOVA and post hoc Tukey tests P le0.05.Results Among the experimental groups subgroup 1C Lateral condensation with bioceramic sealer showed the highest fracture resistance and no significant difference was found between 1C and 2C Single cone GP while subgroup A ZOE showed least resistance among all groups.Conclusion The absence of gutta-percha with bioceramic sealer resulted in reduced fracture resistance. Conversely the use of gutta-percha in conjunction with bioceramic sealer through lateral condensation and single cone techniques enhanced the resistance to fracture in endodontically treated teeth.

Effect of Using Race Evo, EdgeFile X7, and Protaper Gold Rotary File Systems on the Fracture Resistance of Obturated Root Canal (A comparative study)

Aim: The purpose of this research was to compare the fracture resistance of obturated roots prepared by using the Race Evo, Edge File X7, and Protaper Gold file systems.&#x0D; Materials and methods: 60 extracted maxillary first molars were selected, their coronal portions were removed and their working length was calculated. The samples were separated into three experimental groups according to the used NiTi rotary systems and one control group; each group contains 15 samples. Group one remained uninstrumented (control), Groups 2-4 were instrumented by the Race Evo (FKG Dentaire SA, La Chaux-de-Fonds, Switzerland), Edge File X7(Edge Endo; Albuquerque, New Mexico, United States), and Protaper Gold file systems (Dentsply, Maillefer, Switzerland) respectively. After instrumentation, the experimental groups were filled using a single-cone technique. The sealer was mixed according to manufacturing instructions with master Gutta-Percha cones of size 40 (0.04 taper, (Diadent Group International, Korea) for Race Evo and Edge file X7, and size 40 taper 0.06 for the Protaper Gold file system (Dentsply, Maillefer, Switzerland). To set the sealer, the specimens were kept at 37 °C and 100% humidity for two weeks. The root fracture stress was measured in Newtons using Instron universal testing equipment.&#x0D; Results: One Way ANOVA test showed there was a significant difference in the mean value of the fracture resistance between the four groups (P &lt; 0.05). The LSD test was used for the comparison of the multiple groups so the control group showed the highest fracture resistance among groups (p &lt; 0.05). among the experimental groups, the roots prepared by Race Evo showed the highest fracture resistance (p &lt; 0.05), while roots prepared by Protaper Gold and Edge file x7 showed the lowest fracture resistance with a statistically significant difference (p &gt; 0.05)&#x0D;

Uncovering the transcriptional responses of tobacco (Nicotiana tabacum L.) roots to Ralstonia solanacearum infection: a comparative study of resistant and susceptible cultivars

BackgroundTobacco bacterial wilt (TBW) caused by Ralstonia solanacearum is the most serious soil-borne disease of tobacco that significantly reduces crop yield. However, the limited availability of resistance in tobacco hinders breeding efforts for this disease.ResultsIn this study, we conducted hydroponic experiments for the root expression profiles of D101 (resistant) and Honghuadajinyuan (susceptible) cultivars in response to BW infection at 0 h, 6 h, 1 d, 3 d, and 7d to explore the defense mechanisms of BW resistance in tobacco. As a result, 20,711 and 16,663 (total: 23,568) differentially expressed genes (DEGs) were identified in the resistant and susceptible cultivars, respectively. In brief, at 6 h, 1 d, 3 d, and 7 d, the resistant cultivar showed upregulation of 1553, 1124, 2583, and 7512 genes, while the susceptible cultivar showed downregulation of 1213, 1295, 813, and 7735 genes. Similarly, across these time points, the resistant cultivar had downregulation of 1034, 749, 1686, and 11,086 genes, whereas the susceptible cultivar had upregulation of 1953, 1790, 2334, and 6380 genes. The resistant cultivar had more up-regulated genes at 3 d and 7 d than the susceptible cultivar, indicating that the resistant cultivar has a more robust defense response against the pathogen. The GO and KEGG enrichment analysis showed that these genes are involved in responses to oxidative stress, plant–pathogen interactions, cell walls, glutathione and phenylalanine metabolism, and plant hormone signal transduction. Among the DEGs, 239 potential candidate genes were detected, including 49 phenylpropane/flavonoids pathway-associated, 45 glutathione metabolic pathway-associated, 47 WRKY, 48 ERFs, eight ARFs, 26 pathogenesis-related genes (PRs), and 14 short-chain dehydrogenase/reductase genes. In addition, two highly expressed novel genes (MSTRG.61386-R1B-17 and MSTRG.61568) encoding nucleotide-binding site leucine-rich repeat (NBS-LRR) proteins were identified in both cultivars at 7 d.ConclusionsThis study revealed significant enrichment of DEGs in GO and KEGG terms linked to glutathione, flavonoids, and phenylpropane pathways, indicating the potential role of glutathione and flavonoids in early BW resistance in tobacco roots. These findings offer fundamental insight for further exploration of the genetic architecture and molecular mechanisms of BW resistance in tobacco and solanaceous plants at the molecular level.

Interplays between plants and environmental factors determine pullout resistance on different revegetated slopes in mining areas

Plant roots play a crucial role in enhancing soil stability and protecting slopes during ecological restoration, particularly in mining areas where external-soil spray seeding is employed. However, the relationship between plant root pullout resistance and environmental factors on different types of slopes remains unclear. In this study, we investigated the interactions between the pullout resistance of a dominant species, Artemisia gmelinii, and environmental factors on three slope types (rocky, geotechnical, and soil) using multi-group structural equation modeling. Our findings reveal that the pullout resistance of plant roots was strongly influenced by various factors, including but not limited to biological factors such as plant height and biomass. It showed a positive correlation between soil silt content and soil nutrient levels. Notably, the pullout resistance on soil slopes was significantly higher than on rocky slopes. Furthermore, the impact of soil nutrients and texture on pullout resistance was more pronounced on geotechnical and soil slopes compared to rocky slopes. Multi-group structural equation modeling highlighted that among all environmental factors, slope gradient and underground biomass had the most significant influence on pullout resistance across all slope types. Specifically, slope gradient had a greater effect on soil slopes, whereas underground biomass played a more prominent role on rocky and geotechnical slopes. Overall, our study suggests that when implementing external-soil spray seeding in mining areas, it is crucial to consider the interplay between plant roots and environmental factors, including slope properties. This holistic approach is crucial for maximizing the effectiveness of plants in slope protection during eco-engineering projects.

Comparative Analysis of Conventional, Sonic and Laser Activated Irrigation Methods on Fracture Resistance of Endodontically Treated Roots: An in vitro Study

Objective: To compare the effect of different irrigation activation methods on the fracture strength of endodontically treated roots.&#x0D; Methods: Sixty extracted single-rooted mandibular premolars with single canals were used. The specimens were instrumented with ProTaper Next rotary instruments up to size X3 and were randomly split into four groups (n=15) according to final irrigation procedures: Group I: Conventional syringe irrigation with distilled water, Group II: Conventional syringe irrigation with NaOCl and EDTA, Group III: Sonic-activated irrigation with EndoActivator and Group IV: Laser-activated irrigation with Er-Cr: YSGG. Using a single-cone technique, root canals were obturated with gutta-percha and AH Plus root canal sealer. All samples were embedded in self-curing acrylic resin and subjected to axial forces by mechanical compression testing in a universal testing machine at 1 mm/min until fracture occurred. The data were evaluated statistically by using analysis of variance (one-way ANOVA) (P &lt; 0.05).&#x0D; Results: Conventional syringe irrigation with distilled water showed the highest mean value of fracture resistance and Laser-activated irrigation with Er, Cr: YSGG showed the lowest mean value of fracture resistance. In general, statistical analyses showed a non-significant difference between all tested groups (P &gt; 0.05). &#x0D; Conclusions: The fracture resistance of roots treated endodontically was found to be unaffected by different activation methods. However, Laser-activated irrigation with Er-Cr: YSGG resulted in the greatest reduction of fracture resistance in comparison to the other groups.

Biochar-based Bacillus subtilis inoculant for enhancing plants disease prevention: microbiota response and root exudates regulation

Plants regulate root exudates to form the composition of rhizosphere microbial community and resist disease stress. Many studies advocate intervention with biochar (BC) and exogenous microbe to enhance this process and improve plant defenses. However, the mechanism by which BC mediates exogenous microorganisms to enhance root exudate-soil microbial defensive feedback remains unclear. Here, a BC-based Bacillus subtilis SL-44 inoculant (BC@SL) was prepared to investigate the defensive feedback mechanism for plants, which enhanced plant growth and defense more than BC or SL-44 alone. BC@SL not only strengthened the direct inhibition of Rhizoctonia solani Rs by solving the problem of reduced viability of a single SL-44 inoculant but also indirectly alleviated the Rs stress by strengthening plant defensive feedback, which was specifically manifested by the following: (1) increasing the root resistance enzyme activities (superoxide dismutase up to 3.5 FC); (2) increasing the abundance of beneficial microbe in soil (0.38–16.31% Bacillus); and (3) remodeling the composition of root exudates (palmitic acid 3.95–6.96%, stearic acid 3.56–5.93%, 2,4 tert-butylphenol 1.23–2.62%, increasing citric acid 0.94–1.81%, and benzoic acid 0.97–2.13%). The mechanism reveals that BC@SL can enhance the positive regulatory effect between root exudates and microorganisms by optimizing their composition. Overall, BC@SL is a stable and efficient new solid exogenous soil auxiliary, and this study lays the foundation for the generalization and application of green pesticides.Graphical

Cutting resistance assessment for three varieties of cassava roots

Confronted with limited data on the physical and mechanical properties of cassava peel and root to aid machine developers, an efficient model has been developed to assess the peel and root resistance to cutting. The evaluation was done with knife thicknesses of 1.0, 1.5 and 2.0 mm using cassava varieties such as Duade kpakpa, Dudze and Sika bankye as experimental samples for three postharvest delays. The knife penetrated the tuber at 50, 100 and 150 mm away from the proximal end. An average peel thickness obtained was in the range of 1.81 – 3.01 mm. The average diameters recorded ranged from 52.52 mm to 60.40 mm. The cutting resistance assessed for the Duade kpakpa, Sika bankye and Dudze cassava roots were 44.85 N, 50.01N and 53.53 N respectively with no significant differences (P ˃ 0.05). Furthermore, the strength of the peel for the Sika bankye root was 10.31 % higher than that of the Duade kpakpa root and the resistance of the Dudze peel was 6.58 % higher than that of the Sika bankye root. The penetration resistance of the tuber decreased with increasing postharvest delay and increased with increasing knife thickness. The effect of the treatments on the response variables evaluated by factorial analysis showed that significant differences generally decrease with increasing interation. Comparing the results, cutting resistances of 229.02 N, 223.09 N and 204.43 N in maximum were obtained for the Dudze, Sika bankye and Duade kpakpa cassava roots, respectively. The average cutting resistance of Dudze cassava root was 0.24 % higher than the value for Sika bankye root and the value for the Sika bankye root was 7.82 % higher than the Duade kpakpa cassava roots. It is therefore clear that the penetration resistance of the cassava cultivar to compressive cutting could be ranked as (Dudze cassava root &gt; Sika bankye root &gt; Duade kpakpa cassava root). The quantitative assessment by PLSR model under the knife thicknesses (R2 = 0.9689; RMSE = 2.1020) was significantly better than PLSR model under postharvest delay (R2 = 0.7845; RMSE = 4.0183). The technique employed in assessing the cutting resistance gave emphasis to the cultivar differences and provided a measuring sequence and outstanding quantitative analysis.