Number Of Roots Research Articles

Effect of Benzylaminopurine (BAP) and Coconut Water on the Growth of Bucephalandra sp. in Vitro

Tissue culture is useful as a way to obtain seeds in large quantities of seedlings in a short time as an effort to preserve Bucephalandra sp. in nature. The use of PGR has problems such as expensive prices and difficult to find, therefore its use can be replaced by natural PGR that are easy to obtain and have relatively cheap prices such as coconut water. The aim of this research was to determine the effect of Benzylaminopurine (BAP) and coconut water on the growth of Bucephalandra sp. in vitro. This research is a 2-factor factorial experiment using a completely randomized design. The factors are concentration of BAP (0; 1; 3; 5 mg/l) and concentration of coconut water (0; 50; 100 ml/l). The results showed Murashige and Skoog (MS) media with the addition of BAP 1 mg/l + coconut water 50 ml/l (4.11 shoots), BAP 5 mg/l + coconut water 50 ml/l (4.33 shoots), and BAP 3 mg/l (6.22 shoots) were able to provide the number of shoots with high results. The addition of coconut water did not affect the growth of Bucephalandra sp. MS media can grow shoots with the best results in measurements of shoot emergence time, number of roots, shoot and root length. Keywords: Aquatic plant, Endemic plant, Plant growth regulators, Tissue culture.

Effect of pathogen Globisporangium ultimum on plant growth and colonizing bacterial communities

Plants recruit plant-associated microbes from soil to enhance their growth and mitigate the adverse effects of pathogen invasion on plant health. How pathogens impact the interactions of the plant-associated microbes and plant growth is poorly understood. We established S-microsystems (sterile soil inoculated with 101 bacteria isolated from humus soil with Artemisia annua, Oryza sativa or Houttuynia cordata), and N-microsystems (natural soil with these plants) to evaluate the effects of the fungus Globisporangium ultimum on plant growth and their colonizing bacterial communities (CBCs). S-microsystems and N-microsystems were inoculated with and without G. ultimum, respectively. Their seedling growth and CBCs were investigated. Plant height and root numbers in A. annua, O. sativa and H. cordata S-microsystems with G. ultimum were 34.5% and 52.8%, 23.1% and 31.3%, 102.1% and 45.0% higher than those without G. ultimum, respectively. The CBCs were diverse among S-microsystems of A. annua, O. sativa and H. cordata, and the CBC abundances in the three S-microsystems without G. ultimum were higher than those with G. ultimum. The relative abundances of bacterial genera Rhizobium, Pseudomonas, Brevundimonas and Cupriavidus were significantly positively related to plant growth. We determined that the CBCs in A. annua, O. sativa and H. cordata were selective and related to the plant species, and can mitigate disadvantageous influences of G. ultimum on seedling growth. The plants and their CBCs’ abundance and composition were differentially affected by G. ultimum. Our results provide evidence that CBCs promote plant growth due to dynamic changes in the composition and abundance of CBC members, which were affected by plant species and biotic factors.

Modulating the Rhizosphere Medium and Indole − 3−butyric Acid Supply Influence Rooting, Nutrients and Biochemical Constituents and Histological Features of Pedilanthus tithymaloids

AbstractPedilanthus tithymaloids is a popular temperate indoor succulent ornamental plant and is highly marketable. However, the appropriate propagation techniques should be adopted to solve the rooting issues of cuttings. Therefore, the present research was designed to specify the optimal indole − 3−butyric acid (IBA) concentration and rooting medium type for obtaining good rooting and healthy plants. Three IBA levels (0, 1.0, 2.0, and 3.0 g L− 1) and five growing mediums (sand, sand: clay, sand: peat moss, sand: perlite, and sand: vermiculite as 1:1, v/v) were used. The experiment was performed in a randomized complete block design in three replications. The obtained data illustrated that roots number per cutting and rooting percentage reached the maximum values by treating P. tithymaloids cuttings with 3.0 g L− 1 IBA x sand: perlite or sand: vermiculite medium. Cultivating the treated-IBA 3.0 g L− 1 cuttings of P. tithymaloids in sand: perlite showed the most enhancements in plant height, branches number and leaves number, leaf area and herb dry weight. However, the heaviest root dry weight was noticed with IBA 3.0 g L− 1 x sand: peat moss medium. Except sand medium, all other tested media in combination with IBA at 1.0, 2.0–3.0 g L− 1 showed remarkable stimulation effect on chlorophyll content. Sand: vermiculite mixture x IBA at 2.0 g L− 1 was the effective practice for improving carbohydrates percentage. By studying the anatomical changes of the adventitious root of P. tithymaloids plant, it was found that there was an increase in the root diameter, phloem and xylem tissues and xylem vessels diameter due to growing in sand: perlite x 3.0 g L− 1 IBA. In conclusion, dipping the cuttings bases of P. tithymaloids in 3.0 g L− 1 IBA and cultivation in sand: perlite considered the most effective combination practice for better rooting ability and growth and could be advisable for commercial production.

Effect of Different Growing Media and Polybag Sizes on Seeds Germination & Root System of Rough Lemon (Citrus jambhiri Lush.) in Nursery Condition

Aims: The study aimed to evaluate the different growing media & polybag size for seedling growth of rough lemon rootstock. Study Design: The experiment used a Complete Randomized Design (CRD) with three replications. Place and Duration of Study: The study "Effect of Growing Media and Polybag Size on Rough Lemon Seedling Growth" took place in an insect-proof net house at CCS Haryana Agricultural University during the 2021-2022 academic year. Methodology: It comprising of 30 treatment combination i.e., ten different growing media: T1: garden soil; T2: soil: sand (1:1); T3: soil: sand: leafmold (1:1:1); T4: soil: cocopeat (1:1); T5: sand: cocopeat (1:1); T6: soil: sand: cocopeat (1:1:1); T7: soil: FYM (1:1); T8: sand: FYM (1:1); T9: soil: sand: FYM (1:1:1); T10: soil: sand: FYM: cocopeat (1:1:1:1), and the polybags S1: large (30 x 18 cm), S2: medium (21 x 18 cm) & S3: small size (17 x 16 cm) bags. Results: The study found that adding cocopeat to growing media (T4, T5, T6) resulted in earlier germination and higher rates (87-89%) Whereas, T4 to T10 media consists of either FYM, cocopeat for both resulted into more germination 87-89% as compared to controls (T1, T2, T3). The most of the root growth parameters i.e., diameter of tap root(2.45mm), fresh root weight (4.63g) & dry root weight (1.21g) & root: shoot ratio (0.61) improved with the media sand: FYM, soil: FYM, sand: soil: FYM, sand: soil: FYM: cocopeat in equal proportion. However, when media supplemented with cocopeat (T4, T5 & T6 ) also showed appreciable results as compared to soil, sand: soil & soil: sand: leafmold. However, sand: FYM, sand: cocopeat treatments were best in respect of number of primary roots (61.22), root volume (6.44ml). Whereas, sand: soil showed very poor results in all growth aspects. Among polybags sizes, large size (30 x 18cm) bags performed best in most of the germination & root growth parameters. Conclusion: Media enriched with organic matter outperformed sole media across all parameters, while larger polybags produced significantly better results than medium and small sizes.

Rapid Screening of Traditional Rice Cultivars against Drought Using Tolerance Indices of Seedlings

Aim: To screen the traditional cultivars of rice based on the tolerance indices of seedling characteristics. Study Design: The experimental plan used was Complete Randomized Design with 59 treatments (genotypes) and two replications. Place of Study: P.G. Laboratory, Department of Seed Science and Technology, Uttar Banga Krishi Viswavidyalaya, Pundibari, Cooch Behar district, West Bengal, India. Methodology: Seedling was raised in glass in two sets- PEG-mediated drought and normal conditions. Results: All the seedling characters showed highly significant differences among the varieties, different culture conditions as well as interaction between varieties and environment. Most of the cultivars exhibited reduction in number of primary roots per plant, root length, root fresh weight, root dry weight, shoot length, shoot fresh weight and shoot dry weight. Finally the cultivars were grouped under three categories- tolerant, semi-tolerant and susceptible based on mean tolerance index (MTI). The cultivars having ≥ 90.00, MTI Kashiya binni, Jhagri kartik and Garu Chakua were considered as tolerant. Among those three cultivars, Kashiya binni was found to be highly tolerant to drought as its MTI was ≥ 100.00 (114.96). Subsequently 22 cultivars were classified as semi-tolerant. In conclusion, the tolerance indices of seedling traits and subsequently the MTI can be used for rapid screening of rice genotypes for water stress.

Radiographic predictors of post-operative inferior alveolar nerve injury in mandibular third molar surgery

BackgroundCone-beam computed tomography (CBCT) provides additional three-dimensional information on the relationship between the mandibular third molar (M3M) and the inferior alveolar nerve (IAN). As such, CBCT is being increasingly utilized in pre-operative M3M assessment. PurposeThe purpose of the study was to compare the radiographic findings on panoramic and CBCT and their association with post-operative IAN paresthesia. Study design, setting and sampleWe conducted a retrospective cohort study in a sample of patients referred to the Department of Oral and Maxillofacial Surgery at the Royal Dental Hospital of Melbourne, for management of impacted M3Ms. Patients were included in this study if they had one or more high risk findings on OPG, had both OPG and CBCT imaging taken and if at least one M3M had been extracted. Subjects were excluded from this study if their M3Ms were not extracted or if a CBCT was not indicated. Independent variablesThe independent variable was radiographic features identified on OPG (Rood and Shehab’s radiographic signs, root morphology, impaction type, Pell and Gregory classification) and CBCT (number and location of roots, severity of IAN compression and the presence of ankylosis). Outcome variableThe outcome variable was post-operative neurosensory function defined as any reported paresthesia at 2-week post-procedural review. CovariatesThe covariates were patient demographic information (age, sex). AnalysesVariables were initially assessed with univariate logistic regression analysis to determine factors related to developing post-operative paresthesia. Multivariate logistic regression analysis was then used to assess the association between positive univariate study variables and post-operative paresthesia, while adjusting for potential confounders. Covariates were assessed using an omnibus likelihood ratio test and included if they were statistically significant at the p<0.05 level. ResultsThe study sample consisted of 257 subjects who underwent surgical removal of n=386 M3Ms. The mean age was 25.9 (SD = 7.05). The panoramic features of narrowing of canal, diversion of canal and dark/bifid roots were identified as statistically significant associations of post-operative paresthesia. No CBCT features were significantly associated of post-operative paresthesia. Conclusions and RelevanceCertain panoramic features, along with patient age, are significant predictors of IAN paresthesia. CBCT findings were not significant predictors upon multivariate analysis, thus additional CBCT imaging did not significantly improve ability to predict paresthesia.

Respon Pertumbuhan Bibit Single Bud Tanaman Aglaonema Varietas Donna Carmen Terhadap Aplikasi Zat Pengatur Tumbuh dan Pupuk Daun

Ornamental plants now become a trend among the public. The hobby of growing ornamental plants causes the demand for ornamental plant is increased day by day, especially the Aglaonema ornamental plant. In general, the propagation of Aglaonema plants is carried out by using separation of tillers and stem cuttings of 4-7 segments. Due to the high demand for Aglaonema plants, a new method of plant propagation is needed, in order to produce more plants in a short time, by using the single bud nursery method. Aglaonema stem cuttings are difficult to do because Aglaonema plants has bud's dormancy period. Dormancy of Aglaonema plant buds is triggered by apical dominance. The way to break bud dormancy on Aglaonema cuttings is using zpt auxin (Rootone-f). In addition, plants also need nutrients to grow, by providing fertilizer. Fertilization for ornamental plants generally uses foliar fertilizer (Gandasil D). This research was conducted using a Randomized Block Design (RBD) which consisted of 2 factors, the first factor zpt auxin (Rootone-f) which consisted of 4 levels (0 ppm, 100 ppm, 200 ppm, 300 ppm), and the second factor was Gandasil D foliar fertilizer which consisted of 3 treatment levels (0 g/l, 1 g/l, 2 g/l) so there are 12 treatment combinations. Each combination was repeated 3 times, thus forming 36 experimental units. The data were analyzed by analysis of variance (ANOVA) to determine the effect of the treatment and those whose effects were significantly different were further tested using the Ducan Multiple Range (DMRT) with a 95% confidence level. The combination of auxin zpt treatment (Rootone-f) with a concentration of 200 ppm and Gandasil D foliar fertilizer with a concentration of 2 g/l showed the best treatment combination on the parameters of observing fresh weight of Aglaonema plants. The concentration of zpt auxin (Rootone-f) 200 ppm gave the highest average value of shoot length, number of leaves, root length, and number of roots of Aglaonema plants. The concentration of Gandasil D 2 g/l foliar fertilizer gave the highest average leaf length and leaf width of Aglaonema plants.

Effects of plant growth regulators on virus-free plantlet regeneration from Abroma augustum (L.) seeds

Abroma augusta is a popular folk therapeutic plant in Bangladesh, rightly called Devil’s cotton in English. The present study was performed to evaluate the in vitro multiple shoot and root generation of Abroma augustum plants. In vitro seed germination, shoot proliferation and root induction were performed following previous methods. The highest percentage of seed germination was 90±0.0% after seven days of inoculation, after which the plants were sterilized with 0.1% NaOCl for 2 min. The longest shoot length was 7.6±0.6 cm at the MS+2+0.3 mg/L BAP+NAA concentration. The greatest number of roots per shoot was 12.0±1.0 cm, and the greatest length of roots was 6.8±1.6 cm in MS media supplemented with 1.0 mg/L IBA. Both the shoots and roots were vigorous and sufficient in length and number. The present study established an efficient in vitro micropopagation process for Abroma augustum plantlet production through seeds. The findings of the present research will be helpful for rapidly regenerating disease-free plantlets.

Genetic variability and diversity analysis for some agronomic traits of a sweet potato (Ipomoea batatas L.) collection: Insights for breeding superior genotypes

A study was conducted during the 2017 cropping season to assess the genetic variability of selected agronomic traits in sweet potato. A total of 355 sweet potato genotypes (351 from different countries of the world and four check varieties) were evaluated at Tuber Crops Research Sub Centre, Bangladesh Agricultural Research Institution, Bogura, Bangladesh. The experiment was conducted using an augmented randomized complete block design. Findings revealed positive correlations among five agronomic traits, namely the weight of marketable storage root per plant (MRW), weight of storage root per plant (RW), fresh weight of foliage per plant (FW), number of marketable storage root per plant (MRN) and number of storage root per plant (RN) suggesting their suitability for phenotypic selection. There was a positive skewness in most studied traits (FW, MRW, MRW and RW), indicating the presence of a few genotypes with exceptionally high values for those traits. By employing principal component analysis, the most influential traits in sweet potato genotypes were identified for first two components (Dim) showing significant contributions to Dim-1 and Dim-2 collectively accounting for 84.7 % of the total variance. Through cluster analysis, two main clusters (Cluster I and Cluster II) were identified among the studied genotypes, each characterized by distinct trait patterns. Cluster I demonstrated superior performance in all traits, while Cluster II exhibited lower values for all traits. Further sub-clustering within Cluster II (sub-clusters IIA and IIB) revealed additional variations of the collection. The Euclidean distance for inter-cluster (ranging from 7.94 to 11.39) and intra-cluster (ranging from 3.17 to 10.38) were determined utilizing the complete distance method. In restricted maximum likelihood (REML) model, all the traits were significantly influenced by genetic effects, with broad-sense heritability ranging from 32 % to 70 %. The REML displayed high selection accuracy, with values exceeding 91 % for studied traits. The Multi-Trait Genotype-Ideotype Distance Index (MGIDI) identified 18 genotypes, with Moz1.15 emerging as the best-performing genotype under a 5 % selection pressure. Among the selected genotypes, trait uniqueness was highest for FW (54 %), followed by RN (50 %), MRN (18 %), MRW (14 %), and RW (5 %). The selection gain for these top-ranked genotypes varied between 23.2 % and 69.2 %. Findings established the existence of useful genetic variability for selected agronomic traits of sweet potato that could be exploited for breeding, genetics study and conservation.

Low-dose 60Co-γ-ray irradiation promotes the growth of cucumber seedlings by inducing CsSAUR37 expression.

Cucumber (Cucumis sativus L.) is a major vegetable crop grown globally, with a cultivation history of more than 3000years. The limited genetic diversity, low rate of intraspecific variation, and extended periods of traditional breeding have resulted in slow progress in their genetic research and the development of new varieties. Gamma (γ)-ray irradiation potentially accelerates the breeding progress; however, the biological and molecular effects of γ-ray irradiation on cucumbers are unknown. Exposing cucumber seeds to 0, 50, 100, 150, 200, and 250Gy doses of 60Co-γ-ray irradiation, this study aimed to investigate the resulting phenotype and physiological characteristics of seedling treatment to determine the optimal irradiation dose. The results showed that low irradiation doses (50-100Gy) enhanced root growth, hypocotyl elongation, and lateral root numbers, promoting seedling growth. However, high irradiation doses (150-250Gy) significantly inhibited seed germination and growth, decreasing the survival rate of seedlings. More than 100Gy irradiation significantly decreased the total chlorophyll content while increasing the malondialdehyde (MDA) and H2O2 content in cucumber. Transcriptome sequencing analysis at 0, 50, 100, 150, 200, and 250Gy doses showed that gene expression significantly differed between low and high irradiation doses. Gene Ontology enrichment and functional pathway enrichment analyses revealed that the auxin response pathway played a crucial role in seedling growth under low irradiation doses. Further, gene function analysis revealed that small auxin up-regulated gene CsSAUR37 was a key gene that was overexpressed in response to low irradiation doses, promoting primary root elongation and enhancing lateral root numbers by regulating the expression of protein phosphatase 2Cs (PP2Cs) and auxin synthesis genes.

Effect of Plant Growth Substances on Rhizogenesis of Stem Cuttings in Pointed Gourd (Trichosanthes dioica Roxb.)

An experiment was conducted to know the influence of growth regulators IBA, NAA and salicylic acid with different concentrations on rooting of stem cuttings in pointed gourd (Trichosanthes dioica Roxb.) in College of Horticulture, Venkataramannagudem, during the year 2023-2024. The experiment was laid out by following Factorial Randomized Block Design (FRBD) with twenty treatment combinations which consists of two factors, Factor-1: types of cuttings (male and female) and Factor-2: growth substances (10). By adopting standard methodology, observations on root and shoot growth were recorded and statistically analyzed. Studies on the effects of various growth substances on the formation of roots in cuttings of pointed gourd vines showed that IBA at 150 ppm recorded highest percentage of rooting, the longest root, the number of roots per cutting the number of leaves per cutting, the length of the shoot and the percentage of establishment in main field among various growth substances. In comparison to female cuttings, male cuttings showed a considerably higher percentage of rooting, as well as more roots per cutting, longest root, longest shoot, more leaves per cutting and a higher percentage of establishment in the main field. IBA 150 ppm + male cuttings recorded considerably more roots per cutting and the longest root than the other combinations, according to the interaction between growth substance and type of cutting.

Micropropagation, micromorphological evaluation and genetic homogeneity validation of Cucumis collosus (Rottl.) Cogn.: A wild progenitor of melon

Cucumis collosus (Rottl.) Cogn., a wild ancestor of melon cultivars, is an underutilized cucurbit of arid horticulture having nutraceutical and therapeutic significance. C. collosus has great potential for enhancing the genetic base of cultivated melon and other cucurbits. This investigation is the first report on a robust and reliable micropropagation system for C. collosus (var. AHK-119) using nodal explants, followed by micromorphological and genetic stability assessment. More than 95% explants showed axillary shoot-bud induction on MS (Murashige and Skoog, 1962) medium containing 0.5 mg L−1 BAP and developed a maximum of 3.2 ± 0.6 shoots measuring 3.2 ± 0.1 cm length. After evaluating the various parameters, the best shoot multiplication (Shoot Number 34.9 ± 1.7, Shoot Length 6.7 ± 0.9 cm) was achieved on MS medium having half-strength of nitrates (825 mg L−1 NH4NO3 and 950 mg L−1 KNO3) + 0.25 mg L−1 6-benzylaminopurine (BAP) + 0.1 mg L−1 Kinetin (Kin) + 0.1 mg L−1 Indole-3-acetic acid (IAA) + additives (50 mg L−1 ascorbic acid and 25 mg L−1 each of adenine sulfate, L-arginine and citric acid), after 4 weeks. Micro-shoots were rooted using three approaches: In Vitro Continuous Treatment (IVCT), In Vitro Pulse-Treatment (IVPT) and Ex Vitro Pulse Treatment (EVPT). Of these, EVPT approach proved to be most successful in terms of rooting response (80%), root number (6.4 ± 0.9) and root length (5.7 ± 0.7 cm). The foliar micro-morphology analysis of in vitro leaves (IVL), ex vitro leaves (EVL) and mother plant leaves (MPL) demonstrated notable adaptive alterations during the transitional stages and produced structurally stable plants similar to the mother plant. Furthermore, the validation of genetic homogeneity using Start Codon Targeted (SCoT) and Inter Simple Sequence Repeats (ISSR) markers revealed monomorphic banding patterns among the micropropagated and the mother plant. The discussed method can be applied for large-scale commercial production of C. collosus for arid horticulture, urban/vertical farming, breeding purposes and improvement of other cucurbits.

The effect of natural extracts and tea compost in some root growth parameters of Calendula plant under stress by heavy elements

A factorial experiment was carried out in one of the greenhouses to study the ability of the calendula plants to accumulate and resist growth in soil contaminated with toxic elements and plant treatment using plant extracts and treatment with different proportions of bio-organic aqueous extracts in some root growth indicators. The experiment included the use of three factors. The first factor included two types of plant extracts (licorice root and malt grain) in addition to the control treatment, while the second factor was treating the planting soil with two ratios of tea compost (1/5 and 1/10). The third factor was the treatment of Potting soil with the heavy elements copper and lead and their mixture at a concentration of 5 mg kg-1, the experiment applied according R.C.D with three replicate, and each replicate included 3 experimental units. The results of the study showed a significant impact in the characteristics of the root growth. The intervention treatment (malt extract and a 1/5 ratio of tea compost for calendula plants stressed with two elements lead and copper and those not stressed) had a significant advantage in root length and the number of roots with an average of 39.33 cm and 16.67 roots, respectively. While the interaction treatment (licorice extract not treated with tea compost and stressed with copper) achieved an average of 3.292 gm for the dry weight of the root system, the two triple interaction treatments (malt extract, not treated with tea compost and stressed with lead, and treated with licorice extract, not treated with tea compost and stressed with copper) achieved an average of (44.66, 16.320) mg kg soil-1 root content of lead and copper.

On random polynomials with an intermediate number of real roots

For each α ∈ ( 0 , 1 ) \alpha \in (0, 1) , we construct a bounded monotone deterministic sequence ( c k ) k ⩾ 0 (c_k)_{k \geqslant 0} of real numbers so that the number of real roots of the random polynomial f n ( z ) = ∑ k = 0 n c k ε k z k f_n(z) = \sum _{k=0}^n c_k \varepsilon _k z^k is n α + o ( 1 ) n^{\alpha + o(1)} with probability tending to one as the degree n n tends to infinity, where ( ε k ) (\varepsilon _k) is a sequence of i.i.d. (real) random variables of finite mean satisfying a mild anti-concentration assumption. In particular, this includes the case when ( ε k ) (\varepsilon _k) is a sequence of i.i.d. standard Gaussian or Rademacher random variables. This result confirms a conjecture of O. Nguyen from 2019. More generally, our main results also describe several statistical properties for the number of real roots of f n f_n , including the asymptotic behavior of the variance and a central limit theorem.

Respuesta morfológica de plántulas de maíz nativo (Zea mays L.) a ambientes contrastantes de nitrógeno

Nitrogen plays a vital role in plant metabolism, influencing growth and development, particularly in crops like maize (Zea mays L.). This study aimed to evaluate the morphological response of maize seedlings to different nitrogen levels. The design was a completely randomized factorial arrangement of 4 x 2, involving four maize cultivars and two nitrogen levels.The variety Sb 302 Berentsen and three native varieties originating from Tecamachalco, Puebla, Mexico were studied. For a period of 14, 21, 28 and 35 days, seedlings were grown in nutrient solution with 10 % and 100 % nitrogen levels under hydroponic conditions. The results revealed significant variability in seedling morphology, particularly in root architecture and dry weight, between the 10 % and 100 % nitrogen treatments. High coefficients of variation were observed in the lengths of crown and seminal roots, alongside significant correlations between root and seedling dry weights at both nitrogen levels. Additionally, a strong correlation was found between root length and number under the 10 % nitrogen treatment. The results highlight the critical role of nitrogen in maize seedling development and the interaction between nitrogen concentration and maize variety, particularly in primary root length. The study improves understanding of nitrogen's role in optimizing maize growth and suggests strategies to enhance nitrogen use efficiency across different maize varieties. Keywords: cereals, nitrogen use efficiency, root architecture, native varieties.

Effect of gamma irradiation on proliferation and growth of friable embryogenic callus and in vitro nodal cuttings of ugandan cassava genotypes.

Cassava (Manihot esculenta Crantz) production and productivity in Africa is affected by two viral diseases; cassava mosaic disease (CMD) and cassava brown streak disease (CBSD). Induced mutagenesis of totipotent/embryogenic tissues or in vitro plant material can lead to the generation of CMD and/or CBSD tolerant mutants. To massively produce non-chimeric plants timely and with less labor, totipotent cells or tissues are a pre-requisite. This study aimed to determine the effect of gamma radiation on the proliferation and growth of friable embryogenic callus (FEC) and in vitro nodal cuttings respectively. To obtain FEC, 2-6 mm sized leaf lobes of nine cassava genotypes were plated on Murashige and Skoog (MS) basal media supplemented with varying levels (37, 50, 70, 100) μM of picloram for production of organized embryogenic structures (OES). The OES of five cassava genotypes (Alado, CV-60444, NASE 3, NASE 13 and TME 204) were crushed and plated in Gresshoff and Doy (GD) basal media in combination with the amino acid tyrosine in varying concentrations for FEC production. FEC from five cassava genotypes and in vitro nodal cuttings of nine genotypes were irradiated using five different gamma doses (0, 5, 10, 15, 20 and 25 Gy) at a dose rate of 81Gy/hr. The lethal dose (LD)50 was determined using the number of roots produced and flow cytometry was done to determine the ploidy status of plants. The highest production of OES was noted in Alado across varying picloram concentrations, while TME 204 obtained the highest amount of FEC. The irradiated FEC gradually died and by 28 days post irradiation, FEC from all five cassava genotypes were lost. Conversely, the irradiated in vitro nodal cuttings survived and some produced roots, while others produced callus. The LD50 based on number of roots varied from genotype to genotype, but plants remained diploid post-irradiation. Accordingly, the effect of gamma irradiation on Ugandan cassava genotypes (UCGs) was genotype-dependent. This information is foundational for the use of in vitro tissues as target material for cassava mutation breeding.

Evaluating the Effectiveness of Organic Seed Priming Formulations on Germination and Seedling Growth in Acid Lime (Citrus aurantifolia Swingle) cv. Kagzi

The primary objective of this research was to evaluating the effectiveness of organic seed priming formulations on germination and seedling growth in acid lime cv. Kagzi at CCS Haryana Agricultural University, Hisar, during the period 2020-2021. The study comprised six distinct treatments of organic formulations, including beejamrit, cow urine (50% and 100%), cow dung, cow dung slurry, and control (without soaking). The seeds were soaked for a duration of 24 hours. The experiment was conducted using a completely randomized design with three replications. The study findings revealed that the control (without soaking) exhibited the highest germination percentage (45.17) and germination rate (2.38), followed closely by the beejamrit. Furthermore, the beejamrit treatment demonstrated remarkable seedling growth at 180 days after sowing, with the highest values recorded for seedling diameter (3.23 mm), seedling shoot length (36.90 cm), fresh shoot weight (3.26 g), fresh root weight (1.26 g), tap root length (37.57 cm), and survival percentage (91.50). Cow dung slurry also exhibited notable performance in terms of seedling growth parameters at 180 days after sowing with the beejamrit. Cow dung slurry showcased the highest number of secondary roots (50.00), number of leaves per seedling (31.67), leaf chlorophyll content (33.80 SPAD), and nitrogen content (1.52%), closely followed by the beejamrit treatment. Accelerating the rate of germination and growth of lime seedlings has become a top concern for nurserymen and scientists on a national level to reduce expenditures and time for seedling maintenance. For overcoming such types of problems at the nursery stage, contributing to more resilient and sustainable farming practices using organic seed priming is a good alternative against chemical seed priming.

Cone beam computed tomography analysis of the root and canal morphology of the maxillary second molars in a Syrian subpopulation

AimTo investigate the root number and morphology of the maxillary second molars in the Syrian population besides bilateral symmetry, and the effect of gender.Methods250 Cone Beam Computed Tomography (CBCT) images (140 females, 110 males) were examined by two endodontists. The detailed analysis included the number of roots, the number of canals and their configurations, bilateral symmetry, and relation to gender. The evaluation was made according to Vertucci classification by analyzing the CBCT images at all levels (Axial, Coronal, Sagittal, Oblique, and 3D). The Chi-square statistical analysis was performed to compare the canals’ morphologies and bilateral symmetry in both genders via SPSS.ResultThe most common shape of the upper second molars is three roots (90.4%). There were significant differences between males and females regarding the symmetry of the number of roots (P < 0.05). The root canal configuration was mainly Vertucci type I classification in the Distobuccally (93.4%) and palatal root (99.6%). The most common type in the mesial root was type I (29.5%), showing all varieties of Vertucci classifications (except VII, and VIII) percentage of MB2 of 70.5%. The ratio of symmetry was (59.6%) with no significant statistical difference between the genders (P = 0.708).ConclusionMost maxillary second molars in the examined Syrian population were types (II) (associated with MB2 cases with three roots and four canals). In terms of symmetry, the number of roots surpassed the canal configuration. Males had a greater symmetrical tendency in the number of roots than females.

Detection of Quantitative Trait Loci Associated with Alkaline Tolerance Using Recombinant Inbred Line Population Derived from Longdao5 × Zhongyouzao8 at Seedling Stage.

Salt-alkaline stress is one of the most stressful occurrences, causing negative effects on plant development and agricultural yield. Identifying and utilizing genes that affect alkaline tolerance is an excellent approach to accelerate breeding processes and meet the needs for remediating saline-alkaline soil. Here, we employed a mapping population of 176 recombinant inbred lines (RILs) produced from a cross between alkali-tolerant Longdao5 and alkali-sensitive Zhongyouzao8 to identify the quantitative trait loci (QTLs) determining alkali tolerance at the seedling stage. For the evaluation of alkali tolerance, the recovered seedling's average alkali tolerance index (ATI), root number (RN), root length (RL), seedling dry weight (SW), root dry weight (RW), and seedling height (SH) were assessed, together with their relative alkaline damage rate. Under alkaline stress, the ATI was substantially negative connected with the root number, seedling height, seedling dry weight, and root dry weight; however, it was considerably positive correlated with the relative alkaline damage rate of the root number and root dry weight. A total of 13 QTLs for the root number, root length, seedling height, seedling dry weight, root dry weight, and alkali tolerance index under alkaline stress were identified, which were distributed across chromosomes 1, 2, 3, 4, 5, 7, and 8. All of these QTLs formed two QTL clusters for alkali tolerance on chromosome 5 and chromosome 7, designated AT5 and AT7, respectively. Nine QTLs were identified for the relative alkaline damage rate of the root number, root length, seedling height, seedling dry weight, and root dry weight under alkali stress. These QTLs were located on chromosome 2, 4, 6, 7, 8, 9, and 12. In conclusion, these findings further strengthen our knowledge about rice's genetic mechanisms for alkaline tolerance. This research offers clues to accelerate breeding programs for new alkaline-tolerance rice varieties.

On some nominations of children in the history of Russian and Chinese languages

The article is devoted to the history of children’s nominations in Russian and Chinese languages. The difference of time range of factual materials used in the article is due to the particularity of the historical development and the fixation of Russian and Chinese languages. The process reveals a serious typological similarity. This is manifested in the possibility of the formation of a large number of nominations from a small number of ancient roots, stability and preservation of the root material, and also in the fact that the semantics of lexemes in both languages can pass through the same stages of their evolution. The meanings of both languages may contain such semantic components as “slave”, “servant, worker”. There are also differences in semantic development between languages. In Russian, the semantics evolve in the direction of age: “child” “young man”. In Chinese, the process can go in the opposite direction-from naming adults to naming children.