Fungal Root Colonization Research Articles

Fungal root colonization of Puccinellia frigida (Phil.) Johnston, a dominant grass species inhabiting the margins of high-altitude hypersaline Andean wetlands

High-altitude hypersaline Andean wetlands are considered stressful environments by the prevalence of extreme abiotic conditions affecting both plant host and fungal root endophytes. Puccinellia frigida (Phil.) Johnston, a dominant plant species inhabiting the margins of these wetlands over 4000m.a.s.l. in Northwest Argentina, is frequently colonized by fungal root endophytes. Here we examined the nature and dynamics of fungal root colonization on this plant species in three different wetlands (six sampling sites) and two growing seasons. Morphologically diverse septate fungi and chytrids were observed in root samples of P. frigida in all sampling sites, whereas arbuscular mycorrhizal fungi were found only in two sites. The level of colonization of chytrids and septate fungi differed significantly both between sites and seasons. Soil organic matter and electrical conductivity were the only habitat parameters significantly related to the observed differences. The widespread occurrence of non-destructive fungal associations suggest an important role for plant survival in extreme environments.

Short‐chain chitin oligomers from arbuscular mycorrhizal fungi trigger nuclear Ca2+ spiking in Medicago truncatula roots and their production is enhanced by strigolactone

The primary objective of this study was to identify the molecular signals present in arbuscular mycorrhizal (AM) germinated spore exudates (GSEs) responsible for activating nuclear Ca(2+) spiking in the Medicago truncatula root epidermis. Medicago truncatula root organ cultures (ROCs) expressing a nuclear-localized cameleon reporter were used as a bioassay to detect AM-associated Ca(2+) spiking responses and LC-MS to characterize targeted molecules in GSEs. This approach has revealed that short-chain chitin oligomers (COs) can mimic AM GSE-elicited Ca(2+) spiking, with maximum activity observed for CO4 and CO5. This spiking response is dependent on genes of the common SYM signalling pathway (DMI1/DMI2) but not on NFP, the putative Sinorhizobium meliloti Nod factor receptor. A major increase in the CO4/5 concentration in fungal exudates is observed when Rhizophagus irregularis spores are germinated in the presence of the synthetic strigolactone analogue GR24. By comparison with COs, both sulphated and nonsulphated Myc lipochito-oligosaccharides (LCOs) are less efficient elicitors of Ca(2+) spiking in M. truncatula ROCs. We propose that short-chain COs secreted by AM fungi are part of a molecular exchange with the host plant and that their perception in the epidermis leads to the activation of a SYM-dependent signalling pathway involved in the initial stages of fungal root colonization.

Biodiversity of Arbuscular Mycorrhizal (AM) fungi in mangroves of Goa in West India

Seventeen mangrove species of eight families at seven riverine and fringe habitats in Goa West India were surveyed for Arbuscular Mycorrhizal (AM) fungal diversity. Sixteen species were found to be mycorrhizal and one species showed no AM fungal colonization. AM root colonization was recorded at all seven sites and ranged from 6%–77%. Maximum root colonization was recorded in Excoecaria agallocha (77%) and minimum colonization in Avicennia marina (6%). Paris-type colonization was predominant at all sites. Auxiliary cells were recorded in roots of Acanthus ilicifolius, Ceriops tagal and Sonneretia alba. AM fungal root colonization and spore density varied by plant species and site. Site average spore density ranged from 1.84 spores·g−1 to 0.54 spores·g−1 of soil. In total, 28 AM fungal species of five genera, viz. Glomus, Acaulospora, Scutellospora, Gigaspora and Entrophospora, were recovered. Glomus was the dominant genus, three species of which were sporocarpic forms. Maximum site species richness (SR) ranged from 16 to 5. Species richness was maximum in A. ilicifolius where seven species of three genera were recovered. Based on relative abundance (RA) and isolation frequency (IF), two common species, viz. G. intraradices and A. laevis, were recovered from all seven sites.

Arbuscular Mycorrhiza: A Biological Budding for Sustainable Agriculture

Arbuscular mycorrhiza (AM) is possibly the most prevalent constituent of global symbiosis and is created by 70–90% of land plant species with fungi that belong to a monophyletic phylum, the Glomeromycota. The initiation of the AM fungal root colonisation begins with hyphae growing towards the plant root and extensively around it, where they subsequently attach to the root surface. This relationship results in an improved acquisition of nutrients (e.g., phosphate and nitrates) from the soil by the plant partners and, in exchange, allows the Arbuscular Mycorrhizal Fungi (AMF) to obtain the photosynthetically fixed carbon sources (e.g., sugars) necessary for their survival and propagation. AM symbiosis is very common as the fungi involved can colonise a vast taxonomic range of both herbaceous and woody plants, which are geographically cosmopolitan and ubiquitous, occurring over a broad ecological range in plants growing in the arctic, temperate and tropical regions. In this review, the development, host range, functioning of AM and its benefits for sustainable farming will be discussed.

Seasonal Variation in Arbuscular Mycorrhizal Fungi Root Colonization of Cheatgrass (Bromus tectorum), an Invasive Winter Annual

Cheatgrass is a highly invasive winter annual grass that is most aggressive in the semi-arid steppe region of western North America. In this region, cheatgrass invasion becomes so severe that virtual monocultures can result. Due to its strategy for growth from autumn to spring, cheatgrass remains active during winter months when most native vegetation is dormant. This shift in host activity could be important for beneficial soil microbes, particularly the arbuscular mycorrhizal fungi (AMF), as they are adapted for coincidental growth with host plants. Many native plant species that are utilized for restoring areas invaded by cheatgrass associate with AMF, so any reduction in these symbiotic fungi could reduce the successful establishment of desirable plant species. Although cheatgrass is recognized as a facultative associate of AMF, its associations with AMF across seasons and throughout its lifespan are not known. We measured AMF colonization of cheatgrass roots from soon after germination through senescence. We found that cheatgrass remains colonized throughout its life. Colonization drops dramatically once soil temperatures approach freezing, but was highest late in the growth cycle of cheatgrass during flowering and seed set. Colonization by AMF never attained levels comparable to highly mycorrhizal plant species. This indicates that cheatgrass is a poor host for AMF throughout its life, and long-term dominance by cheatgrass could alter AMF in soils. Restoring highly invaded sites quickly following invasion might reduce the negative effects of cheatgrass on this important soil microbial community.

English

The pre-symbiosis of arbuscular mycorrhizal fungi (AMF) is induced by the production of specific plant root exudates. Mycorrhizal fungi symbiotic relationships have many benefits to the plants. These benefits include, improved plant growth and developments, and enhanced plant tolerance to several diseases. Over the pre-symbiotic phase, the root releases essential metabolites necessary for fungal growth and root colonization. Root exudates compounds were reported and identified by several researchers. Root exudates have sugar, amino acids, proteins, carbon, some lipophilic compounds, flavonoids, and other bio-molecules. These compounds were presented as a critical and fundamental signal in plant, fungal and microbe bio-communications in the soil. Root-microbe interactions are continuous occurrences in the biological active soil zone. Mycorrhizal fungi bio-interaction can be classified as positive (symbiotic) to the host or could be negative to the plant. Root exudates may act as messengers that communicate and initiate biological and physical interactions between soil organisms and roots. The currentreview will illustrate the role of root exudates in mycorrhizal fungi association, the major components of root exudates and more focus on the disease control by root exudates derived from plants mycorrhizosphere.   Key words: Mycorrhizal fungi, exudates, bio-communications, root, add volatiles, non-volatiles.

Balancing defense and growth—Analyses of the beneficial symbiosis between Piriformospora indica and Arabidopsis thaliana

The mutualistic interaction between the endophytic and root-colonizing fungus Piriformospora indica and Arabidopsis thaliana is a nice model system to study beneficial and non-benefical traits in a symbiosis. Colonized Arabidopsis plants are taller, produce more seeds and are more resistant against biotic and abiotic stress. Based on genetic, molecular and cellular analyses, Arabidopsis mutants were identified which are impaired in their beneficial response to the fungus. Several mutants are smaller rather than bigger in the presence of the fungus and are defective in defense responses. This includes mutants with defects in defense-signaling components, defense proteins and enzymes, and defense metabolites. The mutants cannot control root colonization and are often over-colonized by P. indica. As a consequence, the benefits for the plants are lost and they try to restrict root colonization by activating unspecific defense responses against P. indica. These observations raise the question as to how the plants balance defense gene activation or development and what signaling molecules are involved. P. indica promotes the synthesis of phosphatidic acid (PA), which binds to the 3-PHOSPHOINOSITIDE-DEPENDENT-KINASE1 (PDK1). This activates a kinase pathway which might be crucial for balancing defense and growth responses. The review describes plant defense compounds which are necessary for the mutualistic interaction between the two symbionts. Furthermore, it is proposed that the PA/PDK1 pathway may be crucial for balancing defense responses and growth stimulation during the interaction with P. indica.

Root colonization and spore abundance of arbuscular mycorrhizal fungi in distinct successional stages from an Atlantic rainforest biome in southern Brazil

The influence of plant functional groups and moderate seasonality on arbuscular mycorrhizal (AM) fungal status (root colonization and spore density) was investigated during 13 consecutive months in a chronosequence of succession in southern Brazil, consisting of grassland field, scrub vegetation, secondary forest and mature forest, in a region of transition from tropical to subtropical zones. AM root colonization and spore density decreased with advancing succession and were highest in early successional sites with grassland and scrub vegetation, intermediary in the secondary forest and lowest in the mature forest. They were little influenced by soil properties, but were sufficiently influenced by the fine root nutrient status and fine root traits among different functional plant groups. AM root colonization and spore density were higher during the favourable plant growth season (spring and summer) than during the less favourable plant growth season (autumn and winter). Spore density displayed significant seasonal variation at all sites, whilst root colonization displayed significant seasonal variation in grassland, scrub and secondary forest, but not in mature forest. The data suggest that (1) different plant functional groups display different relationships with AM fungi, influencing their abundance differentially; (2) plant species from early successional phases are more susceptible to AM root colonization and maintain higher AM sporulation than late successional species; (3) fine root traits and nutrient status influence these AM fungal attributes; and (4) higher AM spore production and root colonization is associated with the season of higher light incidence and temperature, abundant water in soil and higher plant metabolic activity.

Microbial community structure in the rhizosphere of Sophora viciifolia grown at a lead and zinc mine of northwest China

Understanding the effects of heavy metal contamination on microbial activity is important for developing strategies to reclaim sites disturbed by industrial activities. In this study, we investigated arbuscular mycorrhizal (AM) fungi and bacteria associated with Sophora viciifolia growing at a lead and zinc mine in Qinling Mountain, China. Specifically, we measured the extent of AM fungal root colonization, examined microbial community structure by PCR-denaturing gradient gel electrophoresis (PCR-DGGE), and identified the dominant microorganisms. We also measured the amount of glomalin-related soil protein (GRSP) and the association of GRSP with heavy metal ions. We found that AM root colonization decreased, but total GRSP increased with the increase of the lead concentration in soil. The Shannon–Wiener Index (H′) of the AM fungal community showed a significant negative correlation with the available lead concentration (r=0.92, P=0.009). Bacterial community H′ was also negatively correlated with the total and available lead concentrations in the soil (r=0.97, P=0.007 and r=0.92, P=0.025 for total and available lead, respectively). Both, AM fungal and bacterial community structures differed significantly between sites along the pollution gradient. The specific distributions of the two kinds of organisms indicated their differing tolerances to heavy metal. The dominant AM fungi were identified as Glomeraceae, whereas the dominant bacteria belonged to Proteobacteria. The amount of lead bound to GRSP varied from 3.3 to 172.5mgkg−1, which positively correlated with total and available soil lead concentration (r=0.99, P<0.000 and r=0.93, P=0.020 for total soil lead and available soil lead concentration, respectively), thus reducing the bioavailability of heavy metal ions.

Root-colonizing ophiostomatoid fungi associated with dying and dead young Scots pine in Poland

Summary Ophiostomatoid fungi are carried by various bark beetles. However, very little is known about the role of these fungi in conifer roots. We studied ophiostomatoid fungi in roots of dying and dead Pinus sylvestris trees and tested the potential phytotoxicity of some isolates using a sensitive bioassay with Lepidium sativum in Poland. Fungi were identified based on their morphology and DNA sequencing. Three ophiostomatoid fungi, Leptographium procerum, Sporothrix inflata and Ophiostoma pallidulum, were isolated from the roots. The most abundant soil-borne fungus, S. inflata, and relatively rare O. pallidulum were isolated for the first time from roots of dying and dead pine trees. The frequency of S. inflata and O. pallidulum correlated with tree decline. The fungi were isolated more frequently from roots of dead than dying trees. Sporothrix inflata and O. pallidulum slightly reduced the stem and root growth of L. sativum. Leptographium procerum reduced more significantly root than stem growth. This species reduced root elongation 32–54% after 10–17 days of incubation.

Neighboring Deschampsia flexuosa and Trientalis europaea harbor contrasting root fungal endophytic communities

Fungal endophytic communities and potential host preference of root-inhabiting fungi of boreal forest understory plants are poorly known. The objective of this study was to find out whether two neighboring plant species, Deschampsia flexuosa (Poaceae) and Trientalis europaea (Primulaceae), share similar root fungal endophytic communities and whether the communities differ between two sites. The study was carried out by analysis of pure culture isolates and root fungal colonization percentages. A total of 84 isolates from D. flexuosa and 27 isolates from T. europaea were obtained. The roots of D. flexuosa harbored 16 different isolate types based on macromorphological characteristics, whereas only 4 isolate types were found in T. europaea. The root colonization by dark septate and hyaline septate hyphae correlated with isolate numbers being higher in D. flexuosa compared to T. europaea. The different isolate types were further identified on the basis of internal transcribed spacer sequence and phylogenetic analysis. An isolate type identified as dark septate endophyte Phialocephala fortinii colonized 50 % of the T. europaea and 21 % of the D. flexuosa specimens. In addition, Meliniomyces variabilis, Phialocephala sphaeroides, and Umbelopsis isabellina were found colonizing the grass, D. flexuosa, for the first time and Mycena sp. was confirmed as an endophyte of D. flexuosa. Site-specific differences were observed in the abundance and diversity of endophytic fungi in the roots of both study plants, but the differences were not as predominant as those between plant species. It is concluded that D. flexuosa harbors both higher amount and more diverse community of endophytic fungi in its roots compared to T. europaea.

Effects on plant growth and root-knot nematode infection of an endophytic GFP transformant of the nematophagous fungus Pochonia chlamydosporia

Pochonia chlamydosporia (Pc123) is a fungal parasite of nematode eggs which can colonize endophytically barley and tomato roots. In this paper we use culturing as well as quantitative PCR (qPCR) methods and a stable GFP transformant (Pc123gfp) to analyze the endophytic behavior of the fungus in tomato roots. We found no differences between virulence/root colonization of Pc123 and Pc123gfp on root-knot nematode Meloidogyne javanica eggs and tomato seedlings respectively. Confocal microscopy of Pc123gfp infecting M. javanica eggs revealed details of the process such as penetration hyphae in the egg shell or appressoria and associated post infection hyphae previously unseen. Pc123gfp colonization of tomato roots was low close to the root cap, but increased with the distance to form a patchy hyphal network. Pc123gfp colonized epidermal and cortex tomato root cells and induced plant defenses (papillae). qPCR unlike culturing revealed reduction in fungus root colonization (total and endophytic) with plant development. Pc123gfp was found by qPCR less rhizosphere competent than Pc123. Endophytic colonization by Pc123gfp promoted growth of both roots and shoots of tomato plants vs. uninoculated (control) plants. Tomato roots endophytically colonized by Pc123gfp and inoculated with M. javanica juveniles developed galls and egg masses which were colonized by the fungus. Our results suggest that endophytic colonization of tomato roots by P. chlamydosporia may be relevant for promoting plant growth and perhaps affect managing of root-knot nematode infestations.

Effect of edaphic factors and seasonal variation on spore density and root colonization of arbuscular mycorrhizal fungi in sugarcane fields

Sugarcane fields in 14 different study sites were analyzed for the presence of different arbuscular mycorrhizal fungal (AMF) spores. A total of 23 AMF species representing four genera were identified, among which Glomus fasciculatum and G. mosseae were the dominant species. The mean spore density in the root-zone soils of sugarcane plants varied from 119 to 583 per 100 g of soil, and the mean percentage root colonization varied from 60 to 89 %. A study of the effect of edaphic factors on AM spore density and percentage root colonization revealed a positive correlation between pH and AMF spore density and root colonization and a negative correlation between electrical conductivity, nitrogen, and phosphorus. A positive correlation was observed between AMF spore density and root colonization. Season was also found to play a vital role in determining AMF spore density and percentage root colonization, with high spore density and root colonization observed during the summer season and lower spore densities and root colonization during the winter season.

Arbuscular mycorrhizal colonization of giant sequoia (Sequoiadendron giganteum) in response to restoration practices

Interactions with soil microbiota determine the success of restoring plants to their native habitats. The goal of our study was to understand the effects of restoration practices on interactions of giant sequoia Sequoiadendron giganteum with arbuscular mycorrhizal (AM) fungi (Glomeromycota). Natural regeneration of Sequoiadendron is threatened by the absence of severe fires that create forest canopy gaps. Generating artificial canopy gaps offers an alternative tool for giant sequoia restoration. We investigated the effect of regeneration practices, including (i) sapling location within gaps, (ii) gap size and (iii) soil substrate, on AM fungal colonization of giant sequoia sapling roots in a native giant sequoia grove of the Sierra Nevada, California. We found that the extent of AM fungal root colonization was positively correlated with sapling height and light availability, which were related to the location of the sapling within the gap and the gap size. While colonization frequency by arbuscules in saplings on ash substrate was higher relative to saplings in mineral soil, the total AM fungal root colonization was similar between the substrates. A negative correlation between root colonization by Glomeromycota and non-AM fungal species indicated antagonistic interactions between different classes of root-associated fungi. Using DNA genotyping, we identified six AM fungal taxa representing genera Glomus and Ambispora present in Sequoiadendron roots. Overall, we found that AM fungal colonization of giant sequoia roots was associated with availability of plant-assimilated carbon to the fungus rather than with the AM fungal supply of mineral nutrients to the roots. We conclude that restoration practices affecting light availability and carbon assimilation alter feedbacks between sapling growth and activity of AM fungi in the roots.

Response of tomato (Solanum lycopersicum L.) to nursery inoculation with Trichoderma harzianum and arbuscular mycorrhizal fungi under field conditions

The effect of nursery inoculation of tomato (Solanum lycopersicum L.) with Trichoderma harzianum and arbuscular mycorrhizal fungi (AMF) Glomus mosseae on fungal root colonization, plant growth, yield and quality of field-grown tomato was investigated. The four treatments included T. harzianum, AMF, T. harzianum+AMF, and uninoculated control. At mid-harvest, 84 days after transplanting, no interactive effect of the fungi on the external mycelium growth was observed. Inoculation with AMF alone or in combination with T. harzianum increased dry shoot weight by 35% and 30%, respectively, during the first season, and by 30% and 21%, respectively, during the second growing season. Trichoderma harzianum increased the percentage of large fruit by 76% in 2008–2009, whereas AMF increased the percentage of extra-large fruit by 44% in 2009–2010. Similarly, AMF increased total soluble solids by 10%. Inoculated tomato seedlings with T. harzianum and/or AMF significantly increased early yield of tomato, by 10%, 65% and 70%, respectively, during 2008–2009, and by 27%, 36% and 37%, respectively during the 2009–2010 growing season. In conclusion, results of the study suggested that T. harzianum and AMF have the potential to improve growth, early yield and fruit quality of field-grown tomato.

Short- and long-term effects of olive mill wastewater land spreading on soil chemical and biological properties

Olive mill wastewater (OMW) is the main residual product of olive processing and its disposal can represent a relevant environmental issue in Mediterranean countries, where olive oil production is large and concentrated in a short-lasting period. OMW is characterised by high pollutant load, salinity and phytotoxic levels of polyphenols, but also by a high amount of organic compounds and plant mineral nutrients. Therefore, OMW field spreading may represent a low cost contribution to crop fertilisation and soil amendment. Here, we assessed the short- and long-term effects of long-lasting repeated OMW applications on soil chemical and biochemical properties and arbuscular mycorrhizal fungi (AMF). In addition the influence of two OMW management strategies, characterised by different seasonality of spreading (autumn and spring times) was evaluated. Soil was amended by 0 and 80 m3 ha−1 of OMW. Principal coordinate analyses (PCO) together with PERMANOVAs showed that long-lasting repeated OMW spreading: (i) affected the main soil chemical and biochemical parameters in the short-term, whereas did not determine long-term residual effects irrespective of the application times; (ii) decreased AM fungal root colonisation both after autumn and spring OMW applications; (iii) improved arbuscule occurrence in the short- and long-term for both disposal times. Therefore, at least regarding the monitored parameters, we can argue that OMW may be utilised as organic amendment in agriculture under controlled conditions given the short-term negative effects on soil quality, which can be considered negligible after a suitable waiting period.

Mycorrhizal colonization of major banana genotypes in six East African environments

In the densely populated East African highlands (EAH), banana is a major staple food. In the last decade, serious yield declines were observed caused by soil fertility depletion and increased pressure of pests and diseases. Arbuscular mycorrhizal (AM) fungi form symbiosis with plant roots improving nutrients and water absorption, and protecting plants against a number of biotic (e.g. root pathogens) and abiotic (e.g. drought) stresses. Use of crop genetic diversity could be an appropriate option to improve production by using highly mycotrophic genotypes. This study assessed AM fungal root colonization of 18 banana genotypes across 6 locations in East Africa (Rwanda, Burundi, Kenya, DR Congo, Uganda and Tanzania). Soil and root sampling was carried out on each genotype. Data were collected on root colonization frequency and intensity, soil pH, cation exchange capacity and mineral nutrients. The results suggested that (i) high receptivity but low banana genotype specificity to the AM fungi exist among the five genotypes present in all the study sites; (ii) high variability in root colonization exists within the site among a wide range of Musa genotypes: from AA, AAA to AAB, ABB and tetraploids.

AM fungi root colonization increases the production of essential isoprenoids vs. nonessential isoprenoids especially under drought stress conditions or after jasmonic acid application

Previous studies have shown that root colonization by arbuscular mycorrhiza (AM) fungi enhances plant resistance to abiotic and biotic stressors and finally plant growth. However, little is known about the effect of AM on isoprenoid foliar and root content. In this study we tested whether the AM symbiosis affects carbon resource allocation to different classes of isoprenoids such as the volatile nonessential isoprenoids (monoterpenes and sesquiterpenes) and the non-volatile essential isoprenoids (abscisic acid, chlorophylls and carotenoids). By subjecting the plants to stressors such as drought and to exogenous application of JA, we wanted to test their interaction with AM symbiosis in conditions where isoprenoids usually play a role in resistance to stress and in plant defence. Root colonization by AM fungi favoured the leaf production of essential isoprenoids rather than nonessential ones, especially under drought stress conditions or after JA application. The increased carbon demand brought on by AM fungi might thus influence not only the amount of carbon allocated to isoprenoids, but also the carbon partitioning between the different classes of isoprenoids, thus explaining the not previously shown decrease of root volatile isoprenoids in AM plants. We propose that since AM fungi are a nutrient source for the plant, other carbon sinks normally necessary to increase nutrient uptake can be avoided and therefore the plant can devote more resources to synthesize essential isoprenoids for plant growth.

Mycorrhizal responsiveness trends in annual crop plants and their wild relatives—a meta-analysis on studies from 1981 to 2010

Background and aims Year of release of a cultivar reflects the agricultural and breeding practices of its time; we hypothesize that there are differences in mycorrhizal responsiveness of new high yielding and old crop plants and landraces. We evaluated the importance of the year of release on mycorrhizal responsiveness, arbuscular mycorrhizal (AM) fungal root colonization and P efficiency. We also analyzed the effect of experimental treatments, P efficiency (P acquisition and P utilization efficiency) and AM fungal root colonization on a potential mycorrhizal responsiveness trend for year of release.

Establecimiento de plántulas de Quercus rugosa Née inoculadas con hongos micorrizógenos arbusculares en un bosque templado de México

La Cuenca del Río Magdalena es continuamente deforestada, las estrategias de restauración ecológica incluyen a los hongos micorrizógenos arbusculares ya que facilitan el establecimiento y supervivencia de las plántulas. Con el objetivo de identificar las especies de hongos micorrizógenos arbusculares en la rizósfera de Quercus rugosa y evaluar su colonización en las raíces de Q. rugosa, se recolectaron muestras de suelo y raíces en un encinar. Con el fin de evaluar el crecimiento y supervivencia de plántulas de Q. rugosa inoculadas con hongos micorrizógenos arbusculares e introducidas al campo, se colocaron plántulas germinadas en sustrato estéril, en un invernadero en dos tratamientos: ausencia de hongos (suelo nativo esterilizado, - hongos micorrizógenos arbusculares) y presencia de hongos (suelo nativo, + hongos micorrizógenos arbusculares), y posteriormente fueron transplantadas a campo. Las plántulas del encinar presentaron más del 50% de colonización micorrícica y en la rizósfera se identificaron diez morfoespecies de hongos micorrizógenos arbusculares. En invernadero, las plantas + hongos micorrizógenos arbusculares presentaron mayor supervivencia; aunque las variables de crecimiento no presentaron diferencias significativas. La inoculación con hongos micorrizógenos arbusculares incrementó la supervivencia favoreciendo el establecimiento exitoso de plántulas de Q. rugosa en el encinar, lo que respalda su uso en programas de restauración ecológica.