Role In Life Cycle Research Articles

Understanding processes on digital building permits – a case study in South Tyrol

ABSTRACT Building permitting plays a significant role in life cycles of buildings because without a building permit a legal construction fails. However, digitalization in building permit, authorities is rare in a global context and issuing a building permit is primarily a manual and time-consuming process. In current research approaches on digital building permits, the focus lays on the digitalization of building-related regulation checks. Even if the approaches cover an important step in the building permit process, other significant steps such as administrative processes are neglected. In preliminary research, a building permit process model for structuring action-oriented elements was developed and transferred and applied to a Building Information Modelling (BIM)-oriented and process-based web application prototype, which serves as a basis. An empirical study – using qualitative expert interviews in a building permit authority in South Tyrol (Italy) – was conducted to simulate a BIM-oriented building permit review as well as to investigate the building permit processes thoroughly. The results show a classification of the information needed for digital tools supporting the building permit process, building permit processes of the case study authority, and the digital tools used. In the discussion, knowledge gained from a comparison between other building permit processes is presented alongside considerations for follow-up research.

Linking ecosystems, habitats, and biodiversity: from the grand picture to the tiny details, and back

Natural sciences usually proceed through the analysis of facts that are then assembled into a general framework, often called a “theory”. I have tried here to assemble the “tiny facts” that I have uncovered in my career and to organize them into a holistic perspective. I have chosen to start from the “big picture”, i.e., the functioning of ecosystems, to focus then on details regarding the expression of biodiversity, from the role of life cycles in ecosystem functioning, to the way of assessing biodiversity based on the accurate knowledge of its evolution in time. The Historical Biodiversity Index allows to compare the potential biodiversity (all the species recorded from the studied habitat type) with the realized biodiversity (the species found by sampling in that habitat). The study of natural history might lead to unexpected ecological connections, such as the dynamics of plankton (the most important ecological phenomenon of the whole planet) and the composition of resting stage banks, or the keystone role of the interstitial fauna in determining the diversity of plankton. The oceanic realm is in three dimensions and must be considered as a volume rather than as an area. Living systems, though, change constantly and a fourth dimension (time) is crucial to understand their structure and function. The cells of ecosystem functioning, based on connectivity, are proposed as natural spatial units for both management and protection from human impacts.

Epidemic spread of smut fungi (Quambalaria) by sexual reproduction in a native pathosystem

Quambalaria are fungal pathogens of Corymbia, Eucalyptus and related genera of Myrtaceae. They are smut fungi (Ustilaginomycota) described from structures that resemble conidia and conidiophores. Whether these spore forms have asexual or sexual roles in life cycles of Quambalaria is unknown. An epidemic of Q. pitereka destroyed plantations of Corymbia in New South Wales and Queensland (Australia) in 2008. We sampled 177 individuals from three plantations of C. variegata and used AFLPs to test hypotheses that the epidemic was spread by asexual reproduction and dominated by a single genotype. There was high genotypic diversity across ≥600 AFLP loci in the pathogen populations at each plantation, and evidence of sexual reproduction based on neighbour-net analyses and rejection of linkage disequilibrium. The populations were not structured by host or location. Our data did not support a hypothesis of asexual reproduction but instead that Q. pitereka spreads exclusively by sexual reproduction, similar to life cycles of other smut fungi. Epidemics were exacerbated by monocultures of Corymbia established from seed collected from a single provenance. This study showcases an example of an endemic pathogen, Q. pitereka, with a strictly outbreeding life cycle that has caused epidemics when susceptible hosts were planted in large monoculture plantations.

Marketing Investment Information: Differentiation Resource or Secret?

ABSTRACT Objective: drawing on voluntary disclosure theory, the paper’s main goal was to analyze the main effect of marketing intensity and the moderating role of life cycle on disclosure of marketing investments. Method: the sample includes 89 Brazilian companies listed on B3 stock exchange. We collected financial data from two sources, such as Economatica platform and in the explanatory notes and management report from the companies, which we coded through content analysis. We merged these two datasets and analyzed it using multiple linear regression. Results: both the marketing intensity and the life cycle of companies have effects on disclosure of marketing investments. In addition, the birth and growth phases moderate the main effect of marketing intensity, reducing the level of disclosure. This moderation is identified especially in disclosures of qualitative information. Conclusions: the findings support the voluntary disclosure theory based on arguments of judgment-based disclosure. Outcomes showed that when there is a high intensity of marketing investments, disclosure of marketing investments is managed by moving from the status of secrecy in companies in the birth and growth phases of life cycle to the status of differentiation resource in companies in the maturity phase of life cycle.

Marketing Investment Information: Differentiation Resource or Secret?

ABSTRACT Objective: drawing on voluntary disclosure theory, the paper’s main goal was to analyze the main effect of marketing intensity and the moderating role of life cycle on disclosure of marketing investments. Method: the sample includes 89 Brazilian companies listed on B3 stock exchange. We collected financial data from two sources, such as Economatica platform and in the explanatory notes and management report from the companies, which we coded through content analysis. We merged these two datasets and analyzed it using multiple linear regression. Results: both the marketing intensity and the life cycle of companies have effects on disclosure of marketing investments. In addition, the birth and growth phases moderate the main effect of marketing intensity, reducing the level of disclosure. This moderation is identified especially in disclosures of qualitative information. Conclusions: the findings support the voluntary disclosure theory based on arguments of judgment-based disclosure. Outcomes showed that when there is a high intensity of marketing investments, disclosure of marketing investments is managed by moving from the status of secrecy in companies in the birth and growth phases of life cycle to the status of differentiation resource in companies in the maturity phase of life cycle.

Diversification of ergot alkaloids and heritable fungal symbionts in morning glories

Heritable microorganisms play critical roles in life cycles of many macro-organisms but their prevalence and functional roles are unknown for most plants. Bioactive ergot alkaloids produced by heritable Periglandula fungi occur in some morning glories (Convolvulaceae), similar to ergot alkaloids in grasses infected with related fungi. Ergot alkaloids have been of longstanding interest given their toxic effects, psychoactive properties, and medical applications. Here we show that ergot alkaloids are concentrated in four morning glory clades exhibiting differences in alkaloid profiles and are more prevalent in species with larger seeds than those with smaller seeds. Further, we found a phylogenetically-independent, positive correlation between seed mass and alkaloid concentrations in symbiotic species. Our findings suggest that heritable symbiosis has diversified among particular clades by vertical transmission through seeds combined with host speciation, and that ergot alkaloids are particularly beneficial to species with larger seeds. Our results are consistent with the defensive symbiosis hypothesis where bioactive ergot alkaloids from Periglandula symbionts protect seeds and seedlings from natural enemies, and provide a framework for exploring microbial chemistry in other plant-microbe interactions.

Cholesterol Modulates the Interaction between HIV-1 Viral Protein R and Membrane.

Being a major metabolite for maintaining cellular homeostasis, as well as an important structural component in lipid membrane, cholesterol also plays critical roles in the life cycles of some viruses, including human immunodeficiency virus-1 (HIV-1). The involvement of cholesterol in HIV-1 infectivity, assembly and budding has made it an important research target. Viral protein R (Vpr) is an accessory protein of HIV-1, which is involved in many major events in the life cycle of HIV-1. In addition to its multi-functional roles in the HIV-1 life cycle, it is shown to interact with lipid membrane and form a cation-selective channel. In this work, we examined the effect of cholesterol on the interaction of Vpr and lipid membrane. Using calcein release assay, we found that the membrane permeability induced by the membrane binding of Vpr was significantly reduced in the presence of cholesterol in membrane. In addition, using solid-state NMR (ssNMR) spectroscopy, Vpr was shown to experience multiple chemical environments in lipid membrane, as indicated by the broad line shape of carbonyl 13C resonance of Cys-76 residue ranging from 165–178 ppm, which can be attributed to the existence of complex Vpr-membrane environments. We further showed that the presence of cholesterol in membrane will alter the distribution of Vpr in the complex membrane environments, which may explain the change of the Vpr induced membrane permeability in the presence of cholesterol.

Linking the Determinants of Online Shopping Behavior, Full Nest Consumers, and Behavioral Intentions - A Conceptual Review and Framework

Consumer shopping behavior is a complex process, and several factors affect purchase decisions. In recent times, there is a drastic shift in consumer buying behavior due to technological advancements and mobile shopping applications, and consumers are very much concerned about their purchase decisions of what, where, and how to buy. Online shopping outlets use both push and pull marketing strategies for a wide range of products. Hence, online sellers redefined the business model by making consumers actively visit their online stores even though they do not intend to buy anything. Most of the time, the sellers create the demand traffic; also, impulsive purchases are made by the consumers due to the 'online deals' or 'deal of the day.' Several studies have reported that the family life cycle's role is vital in understanding the consumer market for devising marketing strategies to satisfy various consumer groups' needs. The consumer needs want, and preferences change when they move on to the next stage of the family life cycle. Thus, this study aims to draw a theoretical link between the full nest consumers, online shopping behavior determinants, and online shopping behavioral intentions by proposing a conceptual framework with research propositions. The study identified the key determinants that affect the online shopping behavioral intentions, and the appropriate theoretical linkages were provided with respect to the full nest consumers.

A faunistic survey of snails and their infection with digenean trematode cercariae in Bandar-e Anzali at the littoral of the Caspian Sea.

Trematodes are known as a diverse group of endogenous parasites, which snails as their intermediate hosts can dramatically affect parasite transmission dynamics. Snails play a key role in life cycles of digenean trematode. However, there has not been much faunistic surveys in terms of snails' distribution in Iran. The current study was aimed to identify snail's fauna and their current geographic distribution in four regions of Guilan province, Iran. Several species of snails (land and freshwater snails) were obtained from 4 different areas (2018-2019), and then samples were separately transferred to the laboratory. Diagnosis of snails was then performed according to morphometric characteristics including dextral or sinistral shell, shape, color and size of shell. Moreover, radula was stained and then photographed under microscopic examination. Furthermore, 25% of any species were tested for cercarial infection. In total, 2082 snails belonging to 12 species were identified based on the morphological characteristics. Land snails were identified to be Helicella sp. (46.4%), Helix aspersa (34.1%), Helicopsis sp. (8%), Pomatia sp. (6.7%) and Oxyloma elegans (4.8%). Moreover, freshwater snails were Physa acuta (30.6%), Lymnaea auricularia (25.8%), Lymnaea gedrosiana (18.7%), Lymnaea palustris (8.9%), Lymnaea truncatula (8.1%), Planorbis sp. (6.4%) and Lymnaea stagnalis (1.5%). In collected snails, Lymnaea auricularia (0.66%) and Lymnaea gedrosiana (0.45%) were the only snails harboring cercariae (Gymnocephalus cercariae). There was no statistically significant difference between the cercarial infection and snail species (P<0.05). Comprehensive field studies are highly needed for better evaluation of the snail biodiversity in the Caspian Sea region due to the particular climatic conditions and the high prevalence of trematodosis.

Cholesterol-rich lipid rafts both in cellular and viral membrane are critical for caprine parainfluenza virus type3 entry and infection in host cells

Cholesterol-rich lipid rafts have been shown to play important roles in the life cycle of various non-enveloped and enveloped viruses. Deletion of cholesterol from lipid rafts could influence different steps of viral replication cycle including entry, infection, assembly and release. Caprine parainfluenza virus type3 (CPIV3) is a newly identified member of Paramyxoviridae family. CPIV3 is highly prevalence and threatened the goat industry in China. The infection mechanism of CPIV3 is under exploring and still not fully understood, the roles of cholesterol and lipid rafts for CPIV3 infection remains unclear. In this study, we investigated the association of cholesterol and lipid rafts with CPIV3 during the different viral replication stages (binding, entry and infection) in two cells [MDBK and goat bronchial epithelial (GBE) cells]. Methyl-β- cyclodextrin (MβCD) was used to deplete cholesterol from cell and viral membranes. The results showed that MβCD treatment significantly inhibited CPIV3 entry and infection in these two cells with a dose-dependent manner, but didn't impair the binding of CPIV3. Addition of exogenous cholesterol to the cells after MβCD treatment restored the viral infection. In addition, treatment of MβCD only before virus-entry showed inhibitory effect in MDBK cells. Depletion of cholesterol from virion envelop also decreased the entry and infection of CPIV3 in the two cells. Furthermore, lipid rafts isolation test indicated that viral proteins (HN and N) co-localized with lipid rafts during infection in MDBK and GBE cells. Viral N protein co-localized with caveolin-1 (the marker of lipid rafts) in these two cells both at the entry and infection steps, as detected by con-focal laser scanning microscopy test. In conclusion, the results presented here demonstrated that cholesterol rich lipid rafts play an important role in CPIV3 life cycle. The findings give new insights on understanding of the mechanism of CPIV3 infection and provide a new anti-CPIV3 strategy.

No Evidence of Toxoplasma Gondii Exposure in South Australian Koalas (Phascolarctos cinereus)

Infection with the cat-borne parasite Toxoplasma gondii has been detected in numerous Australian marsupials and can lead to severe disease (toxoplasmosis) in some cases. The seroprevalence of Toxoplasma on Kangaroo Island, South Australia has been reported to be higher than the South Australian mainland in macropods, cats, and sheep, suggesting an increased risk of infection on this island. However, Toxoplasma seroprevalence in small- and medium-sized terrestrial mammals was almost zero on the island and did not differ from that on the mainland. We surveyed Toxoplasma seroprevalence in koala (Phascolarctos cinereus) populations on the island and on the mainland and assessed their risk of infection and their role in the life cycle of Toxoplasma. All screened koalas from the island (n = 94) and the mainland (n = 63) were seronegative. This represents the largest Toxoplasma seroprevalence survey in this species and provided sufficient evidence to confidently demonstrate freedom from parasite exposure in both island and mainland populations at the time of the survey. Because koalas are extensively arboreal and predominately consume tree foliage, they appear to be at negligible risk of Toxoplasma infection. Furthermore, as koalas are rarely consumed by cats, we suggest that they have a minor role in the parasite's life cycle.

企业金融化、生命周期与持续性创新——基于行业分类的实证研究

企业创新问题并非仅仅是短期的研发投入，更是具有连续性的长期不断投入的过程。文章基于企业金融化日趋严重的现实背景，利用2007−2017年中国A股非金融上市公司的面板数据，在新结构经济学关于资源禀赋结构的理论框架下实证检验了企业金融化对持续性创新的影响作用，并同时考虑不同生命周期和行业特征的影响机制。研究发现，企业金融化对非金融上市公司的持续性创新表现具有抑制作用−即“挤出”效应，而这种“挤出”效应在企业成长期尤为强烈。随着企业生命周期阶段的延伸，这种抑制作用被削弱。进一步分行业的研究结果显示，不同行业的上市公司，在不同生命周期阶段受企业金融化“挤出”效应的影响存在一定的差异。总体而言，文章拓展了持续性创新的研究情境，为新结构经济学在微观层面的应用和拓展提供了实证证据，对推动我国经济长远发展的路径选择具有一定的参考意义。

The Practice of Islam in America: An Introduction

The Practice of Islam in America: An Introduction

The Practice of Islam in America: An Introduction

The Practice of Islam in America: An Introduction

High-resolution structure of podovirus tail adaptor suggests repositioning of an octad motif that mediates the sequential tail assembly

The sophisticated tail structures of DNA bacteriophages play essential roles in life cycles. Podoviruses P22 and Sf6 have short tails consisting of multiple proteins, among which is a tail adaptor protein that connects the portal protein to the other tail proteins. Assembly of the tail has been shown to occur in a sequential manner to ensure proper molecular interactions, but the underlying mechanism remains to be understood. Here, we report the high-resolution structure of the tail adaptor protein gp7 from phage Sf6. The structure exhibits distinct distribution of opposite charges on two sides of the molecule. A gp7 dodecameric ring model shows an entirely negatively charged surface, suggesting that the assembly of the dodecamer occurs through head-to-tail interactions of the bipolar monomers. The N-terminal helix-loop structure undergoes rearrangement compared with that of the P22 homolog complexed with the portal, which is achieved by repositioning of two consecutive repeats of a conserved octad sequence motif. We propose that the conformation of the N-terminal helix-loop observed in the Sf6-gp7 and P22 portal:gp4 complex represents the pre- and postassembly state, respectively. Such motif repositioning may serve as a conformational switch that creates the docking site for the tail nozzle only after the assembly of adaptor protein to the portal. In addition, the C-terminal portion of gp7 shows conformational flexibility, indicating an induced fit on binding to the portal. These results provide insight into the mechanistic role of the adaptor protein in mediating the sequential assembly of the phage tail.

Hypoxia-inducible factor-1 alpha as a therapeutic target for primary effusion lymphoma.

Primary effusion lymphoma (PEL) is an aggressive B-cell lymphoma with poor prognosis caused by Kaposi’s sarcoma-associated herpesvirus (KSHV). Previous studies have revealed that HIF-1α, which mediates much of the cellular response to hypoxia, plays an important role in life cycle of KSHV. KSHV infection promotes HIF-1α activity, and several KSHV genes are in turn activated by HIF-1α. In this study, we investigated the effects of knocking down HIF-1α in PELs. We observed that HIF-1α knockdown in each of two PEL lines leads to a reduction in both aerobic and anaerobic glycolysis as well as lipid biogenesis, indicating that HIF-1α is necessary for maintaining a metabolic state optimal for growth of PEL. We also found that HIF-1α suppression leads to a substantial reduction in activation of lytic KSHV genes, not only in hypoxia but also in normoxia. Moreover, HIF-1α knockdown led to a decrease in the expression of various KSHV latent genes, including LANA, vCyclin, kaposin, and miRNAs, under both normoxic and hypoxic conditions. These observations provide evidence that HIF-1α plays an important role in PEL even in normoxia. Consistent with these findings, we observed a significant inhibition of growth of PEL in normoxia upon HIF-1α suppression achieved by either HIF-1α knockdown or treatment with PX-478, a small molecule inhibitor of HIF-1α. These results offer further evidence that HIF-1α plays a critical role in the pathogenesis of PEL, and that inhibition of HIF-1α can be a potential therapeutic strategy in this disease.

Host Immune Responses in HIV-1 Infection: The Emerging Pathogenic Role of Siglecs and Their Clinical Correlates

A better understanding of the mechanisms employed by HIV-1 to escape immune responses still represents one of the major tasks required for the development of novel therapeutic approaches targeting a disease still lacking a definitive cure. Host innate immune responses against HIV-1 are key in the early phases of the infection as they could prevent the development and the establishment of two hallmarks of the infection: chronic inflammation and viral reservoirs. Sialic acid-binding immunoglobulin-like lectins (Siglecs) belong to a family of transmembrane proteins able to dampen host immune responses and set appropriate immune activation thresholds upon ligation with their natural ligands, the sialylated carbohydrates. This immune-modulatory function is also targeted by many pathogens that have evolved to express sialic acids on their surface in order to escape host immune responses. HIV-1 envelope glycoprotein 120 (gp120) is extensively covered by carbohydrates playing active roles in life cycle of the virus. Indeed, besides forming a protecting shield from antibody recognition, this coat of N-linked glycans interferes with the folding of viral glycoproteins and enhances virus infectivity. In particular, the sialic acid residues present on gp120 can bind Siglec-7 on natural killer and monocytes/macrophages and Siglec-1 on monocytes/macrophages and dendritic cells. The interactions between these two members of the Siglec family and the sialylated glycans present on HIV-1 envelope either induce or increase HIV-1 entry in conventional and unconventional target cells, thus contributing to viral dissemination and disease progression. In this review, we address the impact of Siglecs in the pathogenesis of HIV-1 infection and discuss how they could be employed as clinic and therapeutic targets.

Unraveling the role of membrane microdomains during microbial infections.

Infectious diseases pose major socioeconomic and health-related threats to millions of people across the globe. Strategies to combat infectious diseases derive from our understanding of the complex interactions between the host and specific bacterial, viral, and fungal pathogens. Lipid rafts are membrane microdomains that play important role in life cycle of microbes. Interaction of microbial pathogens with host membrane rafts influences not only their initial colonization but also their spread and the induction of inflammation. Therefore, intervention strategies aimed at modulating the assembly of membrane rafts and/or regulating raft-directed signaling pathways are attractive approaches for the. management of infectious diseases. The current review discusses the latest advances in terms of techniques used to study the role of membrane microdomains in various pathological conditions and provides updated information regarding the role of membrane rafts during bacterial, viral and fungal infections.

DORMANCY IN THE FORAMINIFERA: A REVIEW

The Foraminifera are a diverse class of protists whose ubiquity in marine environments, small shells, and ease of collection have made them critical tools in bioindicator, bioassay, paleoenvironmental, and paleoceanographic research. Despite the plethora of applications and accompanying literature on foraminifers, many aspects of their biology and ecology remain unexplored. One of these aspects is dormancy, a life-history strategy involving suspension of active life, arrested development, and reduced or suspended metabolic activity, mediated either by internal physiological factors (known as diapause) or exogenous factors (known as quiescence). Dormancy is a widespread adaptation, playing a role in the life cycles of a huge variety of organisms. Yet, despite anecdotal and circumstantial evidence, very little research has directly addressed this aspect of foraminiferal biology. The relatively recent discovery of cryptobiotic propagules has revealed a fundamental role for dormancy in the life cycles of foraminifers, most prominently for dispersal. Moreover, culture studies with environmental applications have shown that post-propagule quiescence (i.e., in juveniles, sub-adults and adults) may be a common response to environmental stressors, allowing rapid recovery of populations following disturbance or otherwise unfavorable conditions. A review of publications on foraminiferal biology revealed that observations indicating the potential for dormancy have been recorded for at least six decades, and that this potential is well represented throughout the class in a variety of forms, suggesting that dormancy may be a basic adaptation in the Foraminifera. If dormancy is as widespread as the literature suggests, its role in structuring foraminiferal assemblages and determining global distributions in the geological past, present, and future is fundamental. Further research into the mechanisms of dormancy will expand understanding of its role in foraminiferal life cycles, and provide new perspectives in the many fields that utilize and apply foraminiferal data.

Protease Inhibitors as Ad-hoc Antibiotics

Background:Proteases are important enzymes that can degrade proteins and are found in animals, plants, bacteria, fungi and viruses. The action of proteases can be controlled by Protease Inhibitors (PIs), chemical or proteinaceous in nature that can block the active site of protease. Since the step catalyzed by proteases may play important role in life cycle of microbes, hindering the action of proteases by PIs may act as therapeutic intervention for microbial infection.Material and Methods:A thorough study was performed and wide range of literature was surveyed to confirm our results of PIs showing antibacterial activity.Results:PIs have shown to be effective drugs against bacterial pathogens, pathogenic viruses- Human Immunodeficiency Virus (HIV), Herpes virus, Hepatitis Virus. PIs have recently been investigated for controlling protozoan parasites. Clinical value of proteases and their inhibitors has been studied inHelicobacter pyloriwhich is the etiologic agent of gastritis.Conclusion:This review is intended to highlight the role of PIs in the Battle against Microbial Pathogens.