The focus of this study was the relation between both gender role self-concept and gender role attitudes with gender-related behavior of interacting partners. We observed gender-typed communication behaviors of mixed-sex and same-sex dyads and measured gender role self-concept and gender role attitudes. Thirty mixed-sex dyads, 19 women dyads, and 15 men dyads participated in a behavior observation study. The results revealed that the activation of gender role self-concept varies depending on the gender of the interacting people. Furthermore, we found that context and gender of the interacting persons moderated the relation between self-concept and behavior. Participants' gender role attitudes did not correlate with their own behaviors but did with their partners' behaviors.