Potential Role Research Articles

MT1JP: A Pivotal Tumor-Suppressing LncRNA and its Role in Cancer Progression and Therapeutic Potential.

Metallothionein 1J pseudogene (MT1JP) is a long non-coding RNA (lncRNA) that functions as a tumor suppressor in various malignancies. Reduced MT1JP expression is associated with increased tumor proliferation, migration, invasion, epithelial-mesenchymal transition (EMT), and treatment resistance in nine cancers, such as gastric cancer, intrahepatic cholangiocarcinoma, hepatocellular carcinoma, and breast cancer. Mechanistically, MT1JP acts as a competitive endogenous RNA (ceRNA) to regulate oncogenic microRNAs (miRNAs), including miR-92a-3p, miR-214-3p, and miR-24-3p. This regulation restores tumor suppressor genes, such as FBXW7, RUNX3, and PTEN, thereby disrupting oncogenic pathways, including PI3K/AKT, Wnt/βcatenin, and p53, promoting apoptosis, and inhibiting tumor progression. Clinically, MT1JP expression correlates with tumor grade, differentiation, TNM stage, lymph node metastasis, and patient prognosis, suggesting its potential as a diagnostic and prognostic biomarker. Furthermore, its therapeutic potential in RNA-based treatments has attracted significant attention. Despite these findings, questions remain regarding its role in epigenetic regulation, transcriptional control, and RNA delivery. This review explores the molecular mechanisms underlying MT1JP, highlighting its clinical relevance and potential as a therapeutic target. Future research should focus on elucidating its role in epigenetic regulation, overcoming challenges in therapeutic delivery, and validating its utility as a biomarker for different cancers. MT1JP holds promise for advancing precision oncology by providing innovative approaches for cancer diagnosis and treatment.

P0132 ACSS2 inhibition alleviates inflammatory signaling and improves epithelial integrity in Inflammatory Bowel Disease in vitro models

Abstract Background Inflammatory bowel disease (IBD), encompassing Ulcerative Colitis (UC) and Crohn’s Disease (CD), is marked by chronic gastrointestinal inflammation stemming from genetic, environmental, immunological, and microbiota factors1. Acetyl-CoA Synthetase Short Chain Family Member 2 (ACSS2) converts acetate into acetyl-CoA and is primarily cytosolic, but translocates to the nucleus under metabolic stress, where it regulates gene transcription and cellular processes2. Although the gastrointestinal microbiota influences acetate levels, the role of ACSS2 in colitis and intestinal inflammation remains poorly understood3. Methods To evaluate ACSS2's role in IBD, we employed in vitro IBD models using Caco-2 and HT-29 intestinal epithelial cell lines. ACSS2 activity was inhibited with an ACSS2 inhibitor, followed by inflammatory stimulation with 100 ng/ml Tumor Necrosis Factor-α (TNF-α) for 30 minutes or 1% Dextran Sodium Sulfate (DSS) for 6 hours. Protein expression levels were assessed via Western blot and immunohistochemistry (IHC) on paraffin-embedded samples from UC patients. The effects of ACSS2 inhibition on inflammatory signaling pathways and cell integrity were assessed using RT-qPCR, immunoblotting, and transepithelial electrical resistance (TEER) measurements. Results Treatment of Caco-2 and HT-29 cells with DSS or TNF-α induced upregulation of ACSS2 protein levels compared to controls. IHC analysis of 30 paired paraffin-embedded colon samples from UC patients revealed higher ACSS2 expression and nuclear localization in inflamed sections compared to non-inflamed regions. Inhibition of ACSS2 activity in stimulated Caco-2 and HT29 cells led to a significant decrease in the gene expression levels of pro-inflammatory cytokines such as Interleukin 8 (IL-8), IL-1β and TNF-α. Additionally, immunoblotting demonstrated reduced phosphorylation of p65 and ERK/MEK, indicating suppression of NF-κB and MAPK inflammatory signaling pathways. To assess the effect of ACSS2 inhibition on cell integrity, TEER assays revealed that ACSS2 inhibition preserved cell integrity in DSS-treated monolayers, accompanied by increased expression of tight junction proteins Occludin and ZO-1, suggesting improved epithelial integrity in the inflammatory context. Conclusion Our findings demonstrate a pro-inflammatory role for ACSS2 in IBD, mediated through its involvement in inflammatory signaling and epithelial integrity. These results highlight ACSS2 as a potential therapeutic target for IBD and warrant further investigations in more advanced models, including human-derived intestinal organoids and in vivo systems, to validate its role in disease pathogenesis and therapeutic potential.

Distribution and functional significance of KLF15 in mouse cerebellum

Kruppel-like factor 15 (KLF15), a member of the KLF family, is closely involved in many biological processes. However, the mechanism by which KLF15 regulates neural development is still unclear. Considering the complexity and importance of neural network development, in this study, we investigated the potent regulatory role of KLF15 in neural network development. KLF15 was detected highly expressed in the cerebellum and enriched in Purkinje cells, with a significant increase in KLF15 expression between 15 and 20 days of neural development. Knockdown of KLF15 led to loss of Purkinje cells and impaired motility in mice. Therefore, our study aims to elucidate the relationship between KLF15 and Purkinje cells in mice, may provide a new research idea for the developmental mechanism of the mouse cerebellum.

Combinatorial phenotypic landscape enables bacterial resistance to phage infection.

Success of phage therapies is limited by bacterial defenses against phages. While a large variety of anti-phage defense mechanisms has been characterized, how expression of these systems is distributed across individual cells and how their combined activities translate into protection from phages has not been studied. Using bacterial single-cell RNA sequencing, we profiled the transcriptomes of ~50,000 cells from cultures of a human pathobiont, Bacteroides fragilis, infected with a lytic bacteriophage. We quantified the asynchronous progression of phage infection in single bacterial cells and reconstructed the infection timeline, characterizing both host and phage transcriptomic changes as infection unfolded. We discovered a subpopulation of bacteria that remained uninfected and determined the heterogeneously expressed host factors associated with protection. Each cell's vulnerability to phage infection was defined by combinatorial phase-variable expression of multiple genetic loci, including capsular polysaccharide (CPS) biosynthesis pathways, restriction-modification systems (RM), and a previously uncharacterized operon likely encoding fimbrial genes. By acting together, these heterogeneously expressed phase-variable systems and anti-phage defense mechanisms create a phenotypic landscape where distinct protective combinations enable the survival and re-growth of bacteria expressing these phenotypes without acquiring additional mutations. The emerging model of complementary action of multiple protective mechanisms heterogeneously expressed across an isogenic bacterial population showcases the potent role of phase variation and stochasticity in bacterial anti-phage defenses.

Decoding CD24: Roles of chemoradiotherapy resistance and potential as therapeutic targets

Decoding CD24: Roles of chemoradiotherapy resistance and potential as therapeutic targets

Neurodevelopmental and Neuropsychiatric Perspectives on Respiratory Control: Understanding Congenital and Developmental Disorders

: Breathing is an automatic process generated by the central nervous system, crucial for the homeostasis of several body processes. This vital process is underpinned by an intricate network in which distinct functional and anatomical factors and structures play a role. Transcription factors (i.e., PHOX2B and Pbx proteins), as well as neuromodulators (i.e., serotonin, noradrenaline, GABA, and glycine), have been demonstrated as implicated in the regulation of breathing. Besides, the several intertwined excitatory and inhibitory brainstem neural circuits comprising the so-called central pattern generator (CPG) have recently demonstrated a potential role of cerebellar structures and circuits in coordinating the complex and coordinated respiratory act in eupnea. A disruption affecting one of these components, which may also occur on a genetic basis, may indeed result in complex and heterogeneous disorders, including neurodevelopmental ones (such as Rett and Prader-Willi syndrome), which may also present with neuropsychiatric and breathing manifestations and potentially lead to sudden infant death syndrome (SIDS). Herein, we discuss the main factors and systems involved in respiratory control and modulation, outlining some of the associated neurodevelopmental disorders (NDDs) deriving from an impairment in their expression/ function. Further studies are needed to deepen our knowledge of the complexity underpinning “breathing” and the relation between respiratory implications and congenital and developmental disorders.

Circulating Tumor Cells Culture: Methods, Challenges, and Clinical Applications.

Circulating tumor cells (CTCs) play a pivotal role in cancer metastasis and hold considerable potential for clinical diagnosis, therapeutic monitoring, and prognostic evaluation. Nevertheless, the limited quantity of CTCs in liquid biopsy samples poses challenges for comprehensive downstream analysis. In vitro culture of CTCs can effectively address the issue of insufficient CTC numbers. Furthermore, research based on CTC cell lines serves as a valuable complement to traditional cancer cell line-based research. While numerous reports exist on CTC in vitro culture and even the establishment of CTC cell lines, the methods used vary, leading to disparate culture outcomes. This review presents the developmental history and current status of CTC in vitro culture research. Additionally, the culture strategies applied in different methods and analyzed the impact of various steps on culture outcomes are compared. Overall, the review indicates that while the short-term culture of CTCs is relatively straightforward, long-term culture success has been achieved for various specific cancer types but still faces challenges. Further optimization of efficient and widely applicable culture strategies is needed. Additionally, ongoing applications of CTC in vitro culture are summarized, highlighting the potential of expanded CTCs for drug susceptibility testing and as therapeutic tools in personalized treatment.

Monte Carlo simulation of ultrafast water flow in nanotubes

Water transport through nanostructures, particularly nanotubes, is a critical area of study with significant implications for filtration, desalination, and energy-related applications. This research investigates the dynamics of water molecules within nanotubes, focusing on the mechanisms that enable ultrafast transport along the nanotube axis. By employing Monte Carlo simulations, the effects of key parameters such as lattice spacing, friction, and molecular interactions on water flux are systematically analyzed. Furthermore, a theoretical model is developed to elucidate the underlying principles of water flow, highlighting the role of molecular interactions and external periodic potentials in driving efficient transport. The findings contribute to a deeper understanding of nanoscale water dynamics and offer valuable insights for optimizing nanotube-based systems in advanced technological applications.

Assessing Renewable Energy Development Potential in Polish Voivodeships: A Comparative Regional Analysis

This work evaluates the renewable energy development potential of Polish voivodeships based on the TOPSIS method and spatial autocorrelation analysis. Data were obtained from the Polish Local Data Bank, covering 22 indicators in the field of economic, social, environmental, and energy related to renewable energy initiatives. The TOPSIS method was applied to construct a synthetic indicator for each voivodeship, facilitating a hierarchical ranking based on their proximity to an ideal solution representing optimal conditions. The results indicate that the Mazowiecki voivodeship leads the list in terms of renewable energy development potential, followed by Małopolskie i Pomorskie, and that this is mainly due to good economic conditions and large investments in renewable energy projects. Spatial autocorrelation analysis yielded a Moran’s I value of –0.1137 with a Z score of 0.303 and a p value of 0.752, suggesting a weak negative spatial autocorrelation that is not statistically significant. This implies that the distribution of renewable energy potential across voivodeships is largely random and is not influenced by spatial proximity. The study concludes that non-spatial factors play a more significant role in renewable energy development potential, offering valuable insights for policymakers and stakeholders to allow them to focus on economic and social variables when promoting renewable energy initiatives in Poland.

Nuclear Factor-κB Signaling Regulates the Nociceptin Receptor but Not Nociceptin Itself.

The nociceptin receptor (NOP) and nociceptin are involved in the pathways of pain and inflammation. The potent role of nuclear factor-κB (NFκB) in the modulation of tumor necrosis factor-α (TNF-α) and interleukin (IL)-1β on the nociceptin system in human THP-1 cells under inflammatory conditions were investigated. Cells were stimulated without/with phorbol-myristate-acetate (PMA), TNF-α, IL-1β, or PMA combined with individual cytokines. To examine NFκB's contribution to the regulation of the nociceptin system, PMA-stimulated cells were treated with NFκB inhibitor BAY 11-7082, JSH-23, or anacardic acid before culturing with TNF-α or IL-1β. NOP and prepronociceptin (ppNOC) mRNA were quantified by RT-qPCR; cell membrane NOP and intracellular nociceptin protein levels were measured by flow cytometry. Phosphorylation and localization of NFκB/p65 were determined using ImageStream. PMA + TNF-α decreased NOP mRNA compared to stimulation with PMA alone, while PMA + IL-1β did not. BAY 11-7082 and JSH-23 reversed the repression of NOP by PMA + TNF-α. TNF-α and IL-1β attenuated PMA's upregulating effects on ppNOC. None of the inhibitors preserved the upregulation of ppNOC in PMA + TNF-α and PMA + IL-1β cultures. TNF-α strongly mediated the nuclear translocation of NFκB/p65 in PMA-treated cells, while IL-1β did not. Proinflammatory cytokines suppressed NOP and ppNOC mRNA in PMA-induced human THP-1 cells. NFκB signaling seems to be an important regulator controlling the transcription of NOP. These findings suggest that the nociceptin system may play an anti-inflammatory role during immune responses.

Evolutionary dynamics and regulatory site analysis of AMP family genes in cattle and sheep.

Ruminants possess a rich repository of natural antimicrobial peptides(AMPs) within their bodies, surpassing those found in humans and mice. These peptides, including Defensin, Cathelicidin, and Lysozyme, are integral to the body's innate and adaptive immune responses and represent promising alternatives to antibiotics with significant application potential. In the present study, we conducted a systematic analysis of 40 Defensins, 38 Cathelicidins, and 61 Lysozymes in cattle and sheep. Our findings revealed that these peptides have retained functional integrity through the evolutionary history of these species. However, they exhibit unique gene duplication and expansion events when compared to humans and mice, indicating their potent roles in cattle and sheep. Notably, the Cathelicidin gene family experienced the most substantial expansion in these ruminants. The newly expanded genes were highly expressed in tissues and organs such as the tongue surface, intestine, mammary gland, and others, exhibiting tissue-specific preferences. This expression pattern is associated with the unique behaviors and high lactation capacity of ruminants. An in vitro bacterial inhibition assay demonstrated that EBD, LALBA, LYSB, and CATHL4 exhibited significant broad-spectrum antibacterial activity. Additionally, loci dB1, dB5, cB2, cB3, and yB1 were pinpointed as key co-regulatory elements in the antimicrobial peptide motifs within cattle mammary epithelial cells. This research illuminates the structure-function relationship and antimicrobial potency of natural AMP genes in cattle and sheep, providing a theoretical foundation for the development of novel veterinary drugs to treat common bacterial diseases in ruminants and for enhancing animal health care. The identified transcriptional regulatory sites offer a new perspective on the molecular regulation of AMP genes expression, which can be leveraged to improve the disease resistance of domestic animals. This work contributes to a broader understanding of the evolution and regulation of AMP genes, with potential applications for animal health and breeding programs.

Exploring the Potent Roles of an Internally Translated Truncated Connexin-43 Isoform.

Connexins are a family of transmembrane proteins that form membrane channels [...].

Analysis of the Integration of Military Themes and Nazi Ideology in the 'Mathematisches Arbeits- Und Lehrbuch' Textbook Series

This study explores the integration of military themes and Nazi ideology within the "Mathematisches Arbeits- Und Lehrbuch" textbook series, which was a prominent tool in Nazi Germany for embedding National Socialist principles in mathematics education. Authored by Otto Zoll, a Nazi Party member, these textbooks were strategically designed to support the regime's objectives, aligning mathematical instruction with ideological and military indoctrination. The paper focuses on the revisions made to the textbooks after 1937, under directives from the Reich Ministry of Education, which infused them with exercises that directly related to military science, genetics, and Germanic folklore. Notably, students were introduced to complex military applications, such as ballistic calculations and strategic logistics, from as early as grade 10, illustrating the regime's efforts to prepare the youth for future roles in the military and as bearers of Nazi ideology. Through this analysis, the paper highlights the profound impact of these educational materials on shaping the ideologies and values of students, underscoring the potent role of education in propagating state ideologies. By dissecting the content of these textbooks, this study offers insights into how education under authoritarian regimes can be manipulated to achieve specific political ends, contributing to a broader understanding of the intersection between education, ideology, and political control during the Nazi era.

Pentyl leaf volatiles promote insect and pathogen resistance via enhancing ketol-mediated defense responses.

Plants emit an array of volatile organic compounds in response to stresses. Six-carbon green leaf volatiles (GLVs) and five-carbon pentyl leaf volatiles (PLVs) are fatty acid-derived compounds involved in intra- and inter-species communications. Unlike extensively studied GLVs, the biological activities of PLVs remain understudied. Maize (Zea mays L.) contains a unique monocot-specific lipoxygenase, ZmLOX6, that is unable to oxidize fatty acids and instead possesses a hydroperoxide lyase-like activity to specifically produce PLVs. Here, we show that disruption of ZmLOX6 reduced resistance to fall armyworm (FAW; Spodoptera frugiperda) and fungal pathogens Colletotrichum graminicola and Cochliobolus heterostrophus. Metabolite profiling revealed that reduced resistance to insects and pathogens was associated with decreased production of PLVs and ketols, including the better studied α-ketol, 9,10-KODA (9-hydroxy-10-oxo-12(Z)-octadecadienoic acid). Exogenous PLV and 9,10-KODA treatments rescued the resistance of lox6 mutants to FAW and the pathogens. Surprisingly, the susceptible-to-herbivory lox6 mutants produced greater levels of wound-induced jasmonates, suggesting potential substrate competition between JA and PLV pathway branches and highlighting a strong role of PLVs in defense to insects. Similarly, likely due to substrate competition between GLV and PLV synthesis pathways, in response to C. graminicola infection, lox6 mutants accumulated elevated levels of GLVs, which promote susceptibility to this pathogen. Mutation of the GLV-producing ZmLOX10 in the lox6 mutant background reversed the susceptibility to C. graminicola, unveiling the contrasting roles of PLVs and GLVs in resistance to this pathogen. Overall, this study uncovered a potent signaling role of PLVs in defense against insect herbivory and fungal pathogens with distinct lifestyles.

Thermal-density-functional-theory approach to quantum thermodynamics

Understanding the thermodynamic properties of many-body quantum systems and their emergence from microscopic laws is a topic of great significance due to its profound fundamental implications and extensive practical applications. Recent advances in experimental techniques for controlling and preparing these systems have increased interest in this area, as they have the potential to drive the development of quantum technologies. In this study, we present a density-functional-theory approach to extract detailed information about the statistics of work and the irreversible entropy associated with quantum quenches at finite temperature. Specifically, we demonstrate that these quantities can be expressed as functionals of thermal and out-of-equilibrium densities, which may serve as fundamental variables for understanding finite-temperature many-body processes. We, then, apply our method to the case of the inhomogeneous Hubbard model, showing that our density-functional-theory-based approach can be usefully employed to unveil the distinctive roles of interaction and external potential on the thermodynamic properties of such a system. Published by the American Physical Society 2024

Polypyrimidine Tract Binding Protein: A Universal Player in Cancer Development.

Polypyrimidine tract binding protein is a 57-Kda protein located in the perinucleolar compartment where it binds RNA and regulates several biological functions through the regulation of RNA splicing. Numerous research articles have been published that address the cellular network and functions of PTB and its isoforms in various disease states. Through an extensive PubMed search, we attempt to summarize the relevant research into this biomolecule. Besides its roles in embryonic development, neuronal cell growth, RNA metabolism, apoptosis, and hematopoiesis, PTB can affect cancer growth via several metabolic, proliferative, and structural mechanisms. PTB overexpression has been documented in several cancers where it plays a role as a novel prognostic factor. The diverse carcinogenic effect opens an argument into its potential role in inhibitory targeted therapy.

Integrated Analysis of Single-Cell and Bulk RNA Sequencing Reveals HSD3B7 as a Prognostic Biomarker and Potential Therapeutic Target in ccRCC.

Clear cell renal cell carcinoma (ccRCC) is the most common kidney malignancy, with a poor prognosis for advanced-stage patients. Identifying key biomarkers involved in tumor progression is crucial for improving treatment outcomes. In this study, we employed an integrated approach combining single-cell RNA sequencing (scRNA-seq) and bulk RNA sequencing (bulk RNA-seq) to identify biomarkers associated with ccRCC progression and prognosis. Single-cell transcriptomic data were obtained from publicly available datasets, and genes related to tumor progression were screened using Monocle2. Bulk RNA-seq data for ccRCC were retrieved from The Cancer Genome Atlas (TCGA) and integrated with scRNA-seq data to explore tumor heterogeneity. We identified 3 beta-hydroxy steroid dehydrogenase type 7 (HSD3B7) as a candidate biomarker for ccRCC, associated with poor overall survival, disease-specific survival, and progression-free interval. Elevated HSD3B7 expression correlated with aggressive clinical features such as advanced TNM stages, histologic grades, and metastasis. Functional studies demonstrated that HSD3B7 promotes cell proliferation, migration, and invasion in vitro, while its silencing significantly inhibits tumor growth in vivo. Our findings reveal that HSD3B7 is a novel biomarker for ccRCC, providing insights into its role in tumor progression and potential as a target for therapy. This study highlights the value of integrating scRNA-seq and bulk RNA-seq data to uncover key regulators of tumor biology and lays the foundation for developing personalized therapeutic strategies for ccRCC patients.

CK2B is a Prognostic Biomarker and a Potential Drug Target for Hepatocellular Carcinoma.

Although casein kinase II subunit beta (CK2B) was previously reported to be involved in human cancers, such as hepatocellular carcinoma (HCC), there has been no systematic assessment of CK2B in HCC. To assess the potential function of CK2B as a prognostic biomarker and possible druggable target in HCC. The Cancer Genome Atlas database was accessed to investigate the potential oncogenic and prognostic roles of CK2B in HCC. Diverse analytical methods were used to obtain a fuller understanding of CK2B, including CIBERSORT, The Tumor Immune Estimation Resource (TIMER), gene set enrichment analyses (GSEA), Kyoto Encyclopedia of Genes and Genomes (KEGG), and gene ontology (GO). Furthermore, the Comparative Toxicogenomic Database (CTD) was used to identify potential drugs to treat CK2B-overexpressing HCC. Patents for these drugs were reviewed using Patentscope® and Worldwide Espacenet®. Upregulated CK2B expression was markedly associated with more aggressive pathological features, including G3, G4 (vs. G1, G2), and T2, T3 (vs. T1). Kaplan-Meier survival curves indicated that patients with HCC with higher expression of CK2B had worse overall survival (P = 0.005), progression-free interval (P = 0.001), and disease-specific survival (P = 0.011). GO and KEGG analysis revealed that CK2B dysregulation affects mitotic chromosome condensation, protein stabilization and binding, regulation of signal transduction of p53 class mediator, and cancer-related pathways. GSEA identified six well-known pathways, including MAPK, WNT, Hedgehog, and TGFβ signaling pathways. Finally, CTD identified six compounds that might represent targeted drugs to treat HCC with CK2B overexpression. A review of patents indicated these compounds showed promising anticancer results; however, whether CK2B interacts with these drugs and improves drug outcomes for patients with HCC was not confirmed. CK2B is a biomarker for HCC prognosis and could be a potential new drug target. Moreover, the association between infiltrating immune cells and CK2B in the HCC tumor microenvironment might provide a solid basis for further investigation and a potent strategy for immunotherapy of HCC.

Nano - Based Therapeutic Strategies in Management of Rheumatoid Arthritis.

Rheumatoid arthritis (RA) is a chronic autoimmune disease, progressively distinctive via cartilage destruction, auto-antibody production, severe joint pain, and synovial inflammation. Nanotechnology represents as one of the utmost promising scientific technologies of the 21st century. It exhibits remarkable potential in the field of medicine, including imaging techniques and diagnostic tools, drug delivery systems and providing advances in treatment of several diseases with nanosized structures (less than 100 nm). Conventional drugs as a cornerstone of RA management including disease-modifying antirheumatic drugs (DMARDS), Glucocorticosteroids, etc are under clinical practice. Nevertheless, their low solubility profile, poor pharmacokinetics behaviour, and non-targeted distribution not only hamper their effectiveness, but also give rise to severe adverse effects which leads to the need for the emergence of nanoscale drug delivery systems. Several types of nano-diagnostic agents and nanocarriers have been identified; including polymeric nanoparticles (NPs), liposomes, nanogels, metallic NPs, nanofibres, carbon nanotubes, nano fullerene etc. Various patents and clinical trial data have been reported in relevance to RA treatment. Nanocarriers, unlike standard medications, encapsulate molecules with high drug loading efficacy and avoid drug leakage and burst release before reaching the inflamed sites. Because of its enhanced targeting specificity with the ability to solubilise hydrophobic drugs, it acts as an enhanced drug delivery system. This study explores nanoparticles potential role in RA as a carrier for site-specific delivery and its promising strategies to overcome the drawbacks. Hence, it concludes that nanomedicine is advantageous compared with conventional therapy to enhanced futuristic approach.

Modified Wave-Front Propagation and Dynamics Coming from Higher-Order Double-Well Potentials in the Allen–Cahn Equations

In this paper, we conduct a numerical investigation into the influence of polynomial order on wave-front propagation in the Allen–Cahn (AC) equations with high-order polynomial potentials. The conventional double-well potential in these equations is typically a fourth-order polynomial. However, higher-order double-well potentials, such as sixth, eighth, or any even order greater than four, can model more complex dynamics in phase transition problems. Our study aims to explore how the order of these polynomial potentials affects the speed and behavior of front propagation in the AC framework. By systematically varying the polynomial order, we observe significant changes in front dynamics. Higher-order polynomials tend to influence the sharpness and speed of moving fronts, leading to modifications in the overall pattern formation process. These results have implications for understanding the role of polynomial potentials in phase transition phenomena and offer insights into the broader application of AC equations for modeling complex systems. This work demonstrates the importance of considering higher-order polynomial potentials when analyzing front propagation and phase transitions, as the choice of polynomial order can dramatically alter system behavior.