Role Of Diosgenin Research Articles

Gut microbiota modification by diosgenin mediates antiepileptic effects in a mouse model of epilepsy.

Diosgenin, a natural steroid saponin, holds promise as a multitarget therapeutic for various diseases, including neurodegenerative conditions. Its efficacy in slowing Alzheimer's disease, Parkinson's disease, multiple sclerosis, and stroke progression has been demonstrated. However, the role of diosgenin in anti-epilepsy and its potential connection to the modulation of the intestinal microbiota remain poorly understood. In this study, exogenous diosgenin significantly mitigated pentylenetetrazole (PTZ)-induced seizures, learning and memory deficits, and hippocampal neuronal injury. 16S ribosomal RNA (16S rRNA) sequencing revealed a reversal in the decrease of Bacteroides and Parabacteroides genera in the PTZ-induced mouse epileptic model following diosgenin treatment. Fecal microbiota transplantation (FMT) experiments illustrated the involvement of diosgenin in modulating gut microbiota and providing neuroprotection against epilepsy. Our results further indicated the repression of enteric glial cells (EGCs) activation and the TLR4-MyD88 pathway, coupled with reduced production of inflammatory cytokines in the colonic lumen, and improved intestinal barrier function in epilepsy mice treated with diosgenin or FMT. This study suggests that diosgenin plays a role in modifying gut microbiota, contributing to the alleviation of intestinal inflammation and neuroinflammation, ultimately inhibiting epilepsy progression in a PTZ-induced mouse model. Diosgenin emerges as a potential therapeutic option for managing epilepsy and its associated comorbidities.

Extraction and Isolation of Tyrosine Ammonia Lyase (TAL) and Diosgenin from <i>Persea americana</i> as Potent Inhibitors of Melanin Synthesis Involving <i>In Vivo</i> Study on Zebrafish Embryos

Tyrosine ammonia lyase is an enzyme in the natural phenol biosynthesis pathway. L-tyrosine is deaminated to p-Coumaric acid with the help of the enzyme tyrosine ammonia lyase. Tyrosine Ammonia Lyase (TAL) is extracted from the leaves of Persea americana. Diosgenin involved in the inhibition of intracellular tyrosinase is extracted along with the enzyme Tyrosine Ammonia Lyase from leaves of Persea americana, significantly increasing the activity of Tyrosine Ammonia Lyase. Diosgenin in the current study on analysis is highly increasing the activity of enzymes. The ability of the enzyme along with diosgenin to increase the activity of the enzyme is analysed in the current study. The enzyme along with diosgenin is used for depigmentation of skin. The methodology of protein separation on treatment with acetone was utilized for the extraction of the enzyme. p-Coumaric acid formation detected using a UV-Visible spectrophotometer at 380nm confirmed the presence of the enzyme. Defatting followed by microwave-assisted extraction and ultrasound-assisted extraction methodology were used for the extraction of diosgenin from leaves of Persea americana. The role of diosgenin in increasing the activity of the enzyme was carried out by analysing the enzyme activity at 410nm. The enzyme-activating ability of diosgenin was compared with that of magnesium sulphate-purified Tyrosine Ammonia Lyase enzyme and sodium sulphate-purified Tyrosine Ammonia Lyase enzyme. Zebrafish embryos were used as model organisms for the conduction of in vivo studies. kojic acid was used as a control in the in vivo studies to compare the effect of the enzyme extracted along with the diosgenin with that of kojic acid.

Diosgenin: An ingress towards solving puzzle for diabetes treatment.

The consumption and composition of food in daily life predict our health in long run. The relation of diabetes to sweets is quite popular. Diabetes hampers the glucose and insulin regulation in the human body by damaging pancreatic β cells. Diabetes has a strong potential towards altering cellular mechanisms of organs causing unlawful performance. Diabetes alters pathways like TLR4, AChE, NF-ĸB, LPL, and PPAR at different sites that affect the normal cellular machinery and cause damage to the local tissue and organ. The long-lasting effect of diabetes was observed in vascular, cardia, nervous, skeletal, reproductive, hepatic, ocular, and renal systems. The increasing awareness of diabetes and its concern has awakened the common people more enthusiastically. Due to rising harm from diabetes, scientific researchers tend to have more eyes toward it. While searching for diabetes solutions, fenugreek diosgenin could pop up with some positive effects in curing the same. Diosgenin helps to lower the scathe of diabetes by modifying cellular pathways in favor of healthy bodily functions. Diosgenin altered the pathways for renewal of pancreatic β cells for better insulin secretion, initiate GLUT4, enhanced DHEA, modify ER-α-mediated PI3K/Akt pathways. Diosgenin can be an appropriate insult for diabetes in a much evolving way for a healthy lifestyle. PRACTICAL APPLICATIONS: Diabetes is one of the most death causing diseases in the medical world. Regrettably the cure of diabetes is yet to be found. Various scientific team working on the same to look after the most appropriate way for diabetes treatment. There is enormous growth of nutraceutical in the market claiming for cure of different metabolic disorders. Among various bioactive compound fenugreek's diosgenin could took a leap over other in curing and preventing the damage caused by diabetes to different organs. The role of diosgenin in curing various metabolic disorders is quite popular from some time. This article also emphasizes over beneficiary effect of diosgenin in curing the damages caused by diabetes by altering cellular metabolism processes. Hence diosgenin could be a better way for researchers to develop a method for diabetes treatment.

Evaluation the hypolipidemic and antioxidant efficacy of diosgenin extracted from fenugreek seeds in experimentally induced oxidative stressed male rats

This study was conducted to assess the antioxidant status and hypolipidemic role of purified diosgenin extracted from fenugreek seeds in hydrogen peroxide (H2O2) and atherogenic diet experimentally induced oxidative stress male rats. Hexane and chloroform were used for the extraction of diosgenin from ground fenugreek seeds. Purification of extracted fraction was carried out on Sephadex LH-20 column using ethyl acetate as eluent, homogenous symmetrical peak was obtained, and thin layer chromatography on silica gel confirmed that purified fraction is diosgenin in compression to standard diosgenin and the calculated Rf values. The antioxidant scavenging activity was carried out using 2, 2, 1 diphenyl- 1- picrylhydrazyl (DPPH) assay, the result showed that purified diosgenin has an antioxidant activity 13.68 μg/ml while the corresponding values for vitamin C and butylated hydroxyl toluene were 8.43 and 7.26 μg/ml respectively. Fifteen adult Albino male rats were randomly and equally divided into three groups, and treated for six weeks as follows; G1 group served as control, G2: Animals of this group were received drinking water containing 0.75% H2O2 and atherogenic diet, Group 3; Rats of this group were given drinking water containing 0.75% H2O2 and atherogenic diet plus 40 mg purified diosgenin/ kg B.W. dissolved in 0.5 ethyl acetate daily by gavages needle. Fasting blood samples were collected by cardiac puncture technique at 0, third and sixth weeks of experiment. The results revealed that rats in G2 exposed to oxidative stress (0.75% H2O2 plus atherogenic diet) showed a significant elevation (P<0.05) in serum total cholesterol (TC), triacylglycerol (TAG), low density lipoprotein- cholesterol (LDL-C) as well as in malondialdehyde (MDA), and significant suppression in serum high density lipoprotein- cholesterol (HDL-C) and glutathione (GSH) concentrations in comparison to the animals in the first group (G1 group). Intubation of 40 mg/kg B.W. of purified diosgenin was caused a significant alteration in the lipid profile of the treated group (G3), manifested by significant reduction (P<0.05) in serum TC, TAG, LDL-C and significant elevation in HDL-C. Antioxidant status also exhibits significant changes manifested by an elevation of serum GSH and reduction in MDA concentration in G3 group animals. This study has shown the significant role of diosgenin as hypolipidemic agent and its role in restores of the oxidative stress.

Diosgenin attenuates neuropathic pain in a rat model of chronic constriction injury.

Diosgenin is a steroidal saponin extract from numerous plants, including Solanum and Dioscorea species, and has been reported to possess neuroprotective activity. However, the role of diosgenin in neuropathic pain remains unclear. The present study examined the effects of diosgenin on allodynia and the levels of inflammatory mediators in rats following neuropathic pain evoked by chronic constriction injury (CCI). In addition, the underlying molecular mechanisms involved in diosgenin‑induced suppression of neuropathic pain were examined. The results of the present study demonstrated diosgenin reversed CCI‑decreased mechanical withdrawal threshold and thermal withdrawal latency. Furthermore, diosgenin inhibited CCI‑induced upregulated levels of the pro‑inflammatory cytokines tumor necrosis factor‑α, interleukin (IL)‑1β and IL‑2, and suppressed oxidative stress induced by CCI in the spinal cord. Furthermore, diosgenin significantly inhibited the expression of phosphorylated‑p38 mitogen activated protein kinase (MAPK) and nuclear factor (NF)‑κB in the spinal cord in CCI rats compared with sham‑operated rats. In conclusion, the present study demonstrated that diosgenin attenuates neuropathicpainin CCI rats by inhibiting activation of the p38 MAPK and NF‑κB signaling pathways. These results implicate diosgenin in the treatment of neuropathic pain, which merits further clinical investigation.

Potential role of diosgenin in prostate cancer therapy

Potential role of diosgenin in prostate cancer therapy