Role In Reproduction Research Articles

Trim66's paternal deficiency causes intrauterine overgrowth.

The tripartite motif-containing protein 66 (TRIM66, also known as TIF1-delta) is a PHD-Bromo-containing protein primarily expressed in post-meiotic male germ cells known as spermatids. Biophysical assays showed that the TRIM66 PHD-Bromodomain binds to H3 N-terminus only when lysine 4 is unmethylated. We addressed TRIM66's role in reproduction by loss-of-function genetics in the mouse. Males homozygous for Trim66-null mutations produced functional spermatozoa. Round spermatids lacking TRIM66 up-regulated a network of genes involved in histone acetylation and H3K4 methylation. Profiling of H3K4me3 patterns in the sperm produced by the Trim66-null mutant showed minor alterations below statistical significance. Unexpectedly, Trim66-null males, but not females, sired pups overweight at birth, hence revealing that Trim66 mutations cause a paternal effect phenotype.

Strigolactones promote flowering by inducing the miR319-LA-SFT module in tomato

Strigolactones are a class of phytohormones with various functions in plant development, stress responses, and in the interaction with (micro)organisms in the rhizosphere. While their effects on vegetative development are well studied, little is known about their role in reproduction. We investigated the effects of genetic and chemical modification of strigolactone levels on the timing and intensity of flowering in tomato (Solanum lycopersicum L.) and the molecular mechanisms underlying such effects. Results showed that strigolactone levels in the shoot, whether endogenous or exogenous, correlate inversely with the time of anthesis and directly with the number of flowers and the transcript levels of the florigen-encoding gene SINGLE FLOWER TRUSS (SFT) in the leaves. Transcript quantifications coupled with metabolite analyses demonstrated that strigolactones promote flowering in tomato by inducing the activation of the microRNA319-LANCEOLATE module in leaves. This, in turn, decreases gibberellin content and increases the transcription of SFT. Several other floral markers and morpho-anatomical features of developmental progression are induced in the apical meristems upon treatment with strigolactones, affecting floral transition and, more markedly, flower development. Thus, strigolactones promote meristem maturation and flower development via the induction of SFT both before and after floral transition, and their effects are blocked in plants expressing a miR319-resistant version of LANCEOLATE. Our study positions strigolactones in the context of the flowering regulation network in a model crop species.

Isolation and Culture of Primary Human Mammary Epithelial Cells.

The mammary gland is a fundamental structure of the breast and plays an essential role in reproduction. Human mammary epithelial cells (HMECs), which are the origin cells of breast cancer and other breast-related inflammatory diseases, have garnered considerable attention. However, isolating and culturing primary HMECs in vitro for research purposes has been challenging due to their highly differentiated, keratinized nature and their short lifespan. Therefore, developing a simple and efficient method to isolate and culture HMECs is of great scientific value for the study of breast biology and breast-related diseases. In this study, we successfully isolated primary HMECs from small amounts of mammary tissue by digestion with a mixture of enzymes combined with an initial culture in 5% fetal bovine serum-DMEM containing the Rho-associated kinase (ROCK) inhibitor Y-27632, followed by culture expansion in serum-free keratinocyte medium. This approach selectively promotes the growth of epithelial cells, resulting in an optimized cell yield. The simplicity and convenience of this method make it suitable for both laboratory and clinical research, which should provide valuable insights into these important areas of study.

A scoping review of strategies for adolescents' sexual and reproductive health role modelling.

Individuals strive to maintain their sexual and reproductive health (SRH) by being exposed to correct information, having access to contraceptives, and promoting safe sex practices. Adolescent SRH promotion efforts should consider the influence of role models. This review explored the availability and nature of strategies and programmes on adolescents' SRH role modelling and described them using a scoping review. Studies were retrieved from four databases and grey literature through a search of 223 studies. The databases included EBSCO-host, Medline, Sabinet, and Pubmed. Data extraction was guided by a data-extraction tool adapted from the JBI Manual for Evidence Synthesis. The characteristics of the selected studies were recorded in a Microsoft spreadsheet. Eleven studies published between 2014 and 2022 were included for the final review and analysed using thematic analysis. Selected articles focused on adolescents' SRH; however, only two studies focused particularly on role modelling. Nonetheless, some aspects of the findings and recommendations presented could be extrapolated to adolescents' SRH role modelling. This includes adolescent-parent communication on SRH, community engagement, mentoring, positive role modelling, and information sharing through media campaigns. There is a lack of literature on SRH role modelling because most studies did not focus on role modelling as an aspect of SRH. Therefore, research needs to be conducted on strategies and programmes focusing on SRH modelling.Contribution:The findings of this scoping review may encourage the development and implementation of strategies and programmes targeting adolescents' SRH throughout diverse communities to promote adolescent SRH.

Paternalistic state care and the dynamic of COVID-19 pandemic governance in Jakarta's rented social housing

The COVID-19 pandemic has reignited debates on care, exposing the vital role of social reproduction despite its ongoing devaluation. Recent studies underscore the urban marginal's multidimensional vulnerabilities during the pandemic, coupled with inadequate state support and excessive policing amid their disproportionate engagements in low-paying care work essential to the city.This study extends the literature by exploring the discourse of care amid the pandemic in Jakarta's rented social housing (rusunawa), Indonesia. As a state-owned settlement hosting displaced residents from informal settlements, rusunawa represents a new form of urban marginality. Based on an ethnography of three rusunawa between 2019 and 2021, I argue that paternalism defines the state's vision and operation of rusunawa. By portraying the residents as vulnerable yet ignorant, the state justifies the combination of disciplines and supports in ‘caring’ for them.The continued paternalistic care during the pandemic, with an intensification of disciplines and assistance, ameliorated the residents' antagonism against the state. Yet, access to state relief programs still hinged upon the residents' invocation of vulnerabilities and personal connection with rusunawa management. Lastly, I demonstrate the residents' intractable desire for a state that embodies a powerful yet nurturing parent, shaping their assessment of the quality of state care.

Molecular Characterization, Expression and In Situ Hybridization Analysis of a Pedal Peptide/Orcokinin-type Neuropeptide in Cuttlefish Sepiella japonica.

Neuropeptide pedal peptide (PP) and orcokinin (OK), which are structurally related active peptides, have been widely discovered in invertebrates and constitute the PP/OK neuropeptide family. They have complex structures and play myriad roles in physiological processes. To date, there have been no related reports of PP/OK-type neuropeptide in cephalopods, which possess a highly differentiated multi-lobular brain. Rapid Amplification of cDNA Ends (RACE) was employed to obtain the open reading frame (ORF) of PP/OK-type neuropeptide in Sepiella japonica (termed as Sj-PP/OK). Various software were used for sequence analysis. Semi-quantitative PCR was applied to analyze the tissue distribution profile, quantitative real-time PCR (qRT-PCR) was used to study spatio-temporal expression throughout the entire growth and development period, and in situ hybridization (ISH) was employed to observe the tissue location of Sj-PP/OK. in the present study, we identified the ORF of Sj-PP/OK. The putative precursor of Sj-PP/ OK encodes 22 mature peptides, of which only tridecapeptides could undergo post-translationally amidated at C-terminus. Each of these tridecapeptides possesses the most conserved and frequent N-terminus Asp-Ser-Ile (DSI). Sequence analysis revealed that Sj-PP/OK shared comparatively low identity with other invertebrates PP or OK. The tissue distribution profile showed differences in the expression level of Sj-PP/OK between male and female. qRT-PCR data demonstrated that Sj-PP/OK was widely distributed in various tissues, with its expression level increasing continuously in the brain, optic lobe, liver, and nidamental gland throughout the entire growth and development stages until gonad maturation. ISH detected that Sj-PP/OK positive signals existed in almost all regions of the optic lobe except the plexiform zone, the outer edge of all functional lobes in the brain, epithelial cells and the outer membrane layer of the accessory nidamental gland. These findings suggest that Sj-PP/OK might play a role in the regulation of reproduction, such as vitellogenin synthesis, restoration, and ova encapsulation. The study indicated that Sj-PP/OK may be involved in the neuroendocrine regulation in cephalopods, providing primary theoretical basis for further studies of its regulation role in reproduction.

АДАПТИВНИЙ ПІДХІД ДО ВИЗНАЧЕННЯ СТАНУ ВУЛИКА ЗА ДОПОМОГОЮ НЕЙРОННИХ МЕРЕЖ ТА АНАЛІЗУ АУДІО

Problem statement. Monitoring the queen bee is crucial for the health and produc-tivity of a bee colony. The queen plays a vital role in reproduction and maintaining the colony's population. Utilizing neural networks, such as CNNs along with sound analysis, can be a valuable tool for monitoring queen bees and assessing their behavior and health within the hive. Purpose. Finding the best way to preprocess audio data and review it is an important task that, if performed well, will help to track the bee hive population and its health state in general. Related work. With the development of deep learning, several studies were done on their application along with sound analysis in bee hive state identi-fication, such as swarming or bee queen detection. Materials and methods. A public la-beled dataset “To bee or not to bee” with bee hive sounds was used for training. It was split by source and then cut into pieces 4 seconds each. Then, CNN models were trained using different audio feature extraction methods, such as MFCCs and STFT. First, it was trained on a first audio set, and then trained models were utilized to analyze their per-formance on the evaluation set of the second audio set. Results and discussion. According to the training and evaluation results, MFCCs-based models have given constant good results, and when used on a limited audio dataset, pre-trained model showed better per-formance than the one trained from zero. Conclusions. The experiment showed that MFCCs is a better performant feature extraction method for a task of the bee hive sounds analysis and bee queen presence identification. Also, even though training a model on a full audio set results in better performance, pre-trained models can detect a bee queen absence in another hive even after an additional training on a limited audio dataset.

Vegetative and reproductive phenology in seasonal climate vegetation: phenological complementarity between woody and herbaceous plants in the Brazilian Chaco

Plant growth and reproduction patterns may be closely associated with environmental seasonality, but the high diversity of plant strategies has revealed complex phenological patterns. Here, we evaluate the occurrence, duration, and seasonality of vegetative and reproductive phenophases of the Brazilian Chaco vegetation. In addition, we summarize the results of a systematic literature review about the phenology of herbaceous and woody plants in different types of seasonal vegetation in the South American Dry Diagonal. Woody plants have a high intense leaf fall and low leaf flush during the dry season in the Brazilian Chaco, whereas in herbaceous plants these phenophases have more fluctuations. In both types, flowering and fruiting are continuous, not uniform, and mainly concentrate during the rainy season. However, woody species present two intensity peaks independent of rainfall, mean temperature, photoperiod, or soil water availability. In turn, in herbaceous species, flowering and fruiting responded to local climatic variables and photoperiod, presenting a peak of intensity during the rainy season. The rainy season plays an important role in plant growth and reproduction in seasonal climates around the world, mainly due to the energetic demand for plants. Like other vegetation types with seasonal climates, particularly in the South American Dry Diagonal, the Brazilian Chaco presents a wide range of phenological patterns, with generally different patterns between woody and herbaceous species. Community-level phenological studies conducted in areas with a seasonal climate have globally focused only on reproduction in relation to vegetative phenology, mostly ignoring herbaceous flora. Here, we emphasize that the phenological complementarity between the woody and herbaceous components is of great importance in maintaining the availability of resources.

Striving for control over migrant labour and lives: Indonesian factory workers in Taiwan

This article explores how Indonesian factory workers in Taiwan strive to regain control over time, space, meaning, and dignity in the face of their exploitation, precarity, and racialisation. Drawing on ethnographic insight, I investigate migrant workers’ subjective practices both inside and outside their workplaces. The major contribution to labour mobility regime analysis lies in conceptualising how migrant workers exert agency on an everyday level, beyond formal labour organising. The focus on the everyday brings me, on the one hand, to labour processes at different Taiwanese workplaces that employ migrant workers. On the other, it brings me to the sphere of daily reproduction, that is, time outside waged labour. The article speaks to the central concern of this themed issue, namely theorising the role of social reproduction within labour mobility regimes, as I address the inseparable spheres of production and reproduction as sites of control and agency. I show that, on the shopfloor, Indonesian migrant workers’ practices of regaining control often remain individualised. It is in the sphere of daily reproduction where Indonesian factory workers organise collectively. The workers’ practices are rich and creative, but at the same time they are ambiguous and can result in consent, compliance, or conflict with capital’s attempt to seek profit from migrant labour. Nevertheless, they reveal migrant workers’ interests and desires as well as a (subtle) refusal of their conditions and of the control over their work and lives. This refusal defies victimising representations of migrant labour and paternalistic approaches to migrant workers’ protection.

Identification and characterization of uridine diphosphate glycosyltransferases in Dioryctria abietella, with an emphasis on putative roles in olfaction and reproduction

AbstractMultiple physiological processes are involved in the interactions between the herbivore Dioryctria abietella and Pinaceae plants, with a key adaptation being the metabolic detoxification of host plant defensive substances. Moreover, the synthetic insecticides applied to control this coneworm are also shaping genes related to detoxification, such as the uridine diphosphate (UDP) glycosyltransferase (UGT) genes. Yet, the molecular mechanisms underpinning the resistance of D. abietella to toxic compounds remain unknown. In this study, we present an important UGT gene family involved in the detoxification mechanisms of D. abietella. Combining bioinformatic and transcriptomic approaches, a total of 37 UGT‐coding genes were identified from the transcriptome of D. abietella, with 20 full‐length sequences that shared high homology with UGTs found in other pyralid moths. These DabiUGTs were phylogenetically clustered into 12 subfamilies, with the three small clusters in the UGT33 and UGT40 clades mainly contributing to the size of the UGT gene repertoire in D. abietella. Expression profiles obtained from RNA sequencing (RNA‐Seq), reverse transcription PCR (RT‐PCR) and quantitative real‐time PCR (qPCR) revealed that over half of DabiUGTs had a broad tissue expression profile, with 28, 28, 31 and 35 genes detected separately in antennae, legs, abdomens and reproductive tissues. Notably, DabiUGT20, DabiUGT25, DabiUGT28 and DabiUGT34 were all significantly enriched in the antennae. Several DabiUGTs had significant expression levels in reproductive tissues, including DabiUGT6, DabiUGT9, DabiUGT24, DabiUGT26 and DabiUGT32 in female accessory glands, DabiUGT15 in male accessory glands and DabiUGT25 in male testes. Altogether, our results identify candidate DabiUGTs for mediating olfaction and reproduction in D. abietella, warranting further investigation into the roles of DabiUGTs in pesticide resistance, odorant detection and reproduction.

UDP-glucosyltransferase 71C4 controls the flux of phenylpropanoid metabolism to shape cotton seed development

Seeds play a crucial role in plant reproduction, making it essential to identify genes that affect seed development. In this study, we focused on UDP-glucosyltransferase 71C4 (UGT71C4) in cotton, a member of the glycosyltransferase family that shapes seed width and length, thereby influencing seed index and seed cotton yield. Overexpression of UGT71C4 results in seed enlargement owing to its glycosyltransferase activity on flavonoids, which redirects metabolic flux from lignin to flavonoid metabolism. This shift promotes cell proliferation in the ovule via accumulation of flavonoid glycosides, significantly enhancing seed cotton yield and increasing the seed index from 10.66 g to 11.91 g. By contrast, knockout of UGT71C4 leads to smaller seeds through activation of the lignin metabolism pathway and redirection of metabolic flux back to lignin synthesis. This redirection leads to increased ectopic lignin deposition in the ovule, inhibiting ovule growth and development, and alters yield components, increasing the lint percentage from 41.42% to 43.40% and reducing the seed index from 10.66 g to 8.60 g. Our research sheds new light on seed size development and reveals potential pathways for enhancing seed yield.

MALAYSIA’S INDIGENOUS COMMUNITY IN THE MEDIA: DECONSTRUCTING THE RHETORIC OF OTHERING IN DISCOURSE

Media possess the ultimate power to shape cognition of the society and plays a fundamental role in the (re)production of elements of biasness and discrimination in contemporary society. In the field of ethnic affair, the news media become the most important source of access to information concerning the indigenous community. In this context, the study aims to unravel the projection of Malaysian indigenous community; the Orang Asli in a selected news media through the application of van Dijk’s Theory of Semantic Macrostructure. Attention is paid to the deployment of rhetorical structures and how these structures scrutinised in structuring and shaping the minds of the general public concerning the Orang Asli community. Findings reveal the hidden ideology beneath the tactical use of rhetorical structures whereby the dominant and the elites are shown to be maintaining their power while the Orang Asli community are contrasted with perpetuated stereotypes and prejudice in various degrees. The study concludes that the community is commonly viewed through unconstructive themes such as being dependant and traditional, and this study strongly opposes the blatancy of media while at the same time, calls for a more neutral and subtle representation of the community.

AN ADAPTIVE APPROACH TO THE BEE HIVE STATE IDENTIFICATION USING NEURAL NETWORKS AND AUDIO ANALYSIS

Monitoring the queen bee is crucial for the health and productivity of a bee colony. The queen plays a vital role in reproduction and maintaining the colony's population. Utilizing neural networks, such as CNNs along with sound analysis, can be a valuable tool for monitoring queen bees and assessing their behavior and health within the hive. Finding the best way to preprocess audio data and review it is an important task that, if performed well, will help to track the bee hive population and its health state in general. According to the training and evaluation results, MFCCs-based models have given constant good results, and when used on a limited audio dataset, pre-trained model showed better performance than the one trained from zero.

DENND1A desensitizes granulosa cells to FSH by arresting intracellular FSHR transportation.

Polycystic ovary syndrome (PCOS) is a complex disorder. Genome-wide association studies (GWAS) have identified several genes associated with this condition, including DENND1A. DENND1A encodes a clathrin-binding protein that functions as a guanine nucleotide exchange factor involved in vesicular transport. However, the specific role of DENND1A in reproductive hormone abnormalities and follicle development disorders in PCOS remain poorly understood. In this study, we investigated DENND1A expression in ovarian granulosa cells (GCs) from PCOS patients and its correlation with hormones. Our results revealed an upregulation of DENND1A expression in GCs from PCOS cases, which was positively correlated with testosterone levels. To further explore the functional implications of DENND1A, we generated a transgenic mouse model overexpressing Dennd1a (TG mice). These TG mice exhibited subfertility, irregular estrous cycles, and increased testosterone production following PMSG stimulation. Additionally, the TG mice displayed diminished responsiveness to FSH, characterized by smaller ovary size, less well-developed follicles, and abnormal expressions of FSH-priming genes. Mechanistically, we found that Dennd1a overexpression disrupted the intracellular trafficking of follicle stimulating hormone receptor (FSHR), promoting its internalization and inhibiting recycling. These findings shed light on the reproductive role of DENND1A and uncover the underlying mechanisms, thereby contributing valuable insights into the pathogenesis of PCOS and providing potential avenues for drug design in PCOS treatment.

On Connective Tissue Mast Cells as Protectors of Life, Reproduction, and Progeny.

The connective tissue mast cell (MC), a sentinel tissue-residing secretory immune cell, has been preserved in all vertebrate classes since approximately 500 million years. No physiological role of the MC has yet been established. Considering the power of natural selection of cells during evolution, it is likely that the MCs exert essential yet unidentified life-promoting actions. All vertebrates feature a circulatory system, and the MCs interact readily with the vasculature. It is notable that embryonic MC progenitors are generated from endothelial cells. The MC hosts many surface receptors, enabling its activation via a vast variety of potentially harmful exogenous and endogenous molecules and via reproductive hormones in the female sex organs. Activated MCs release a unique composition of preformed and newly synthesized bioactive molecules, like heparin, histamine, serotonin, proteolytic enzymes, cytokines, chemokines, and growth factors. MCs play important roles in immune responses, tissue remodeling, cell proliferation, angiogenesis, inflammation, wound healing, tissue homeostasis, health, and reproduction. As recently suggested, MCs enable perpetuation of the vertebrates because of key effects-spanning generations-in ovulation and pregnancy, as in life-preserving activities in inflammation and wound healing from birth till reproductive age, thus creating a permanent life-sustaining loop. Here, we present recent advances that further indicate that the MC is a specific life-supporting and progeny-safeguarding cell.

House dust-derived mixtures of organophosphate esters alter the phenotype, function, transcriptome, and lipidome of KGN human ovarian granulosa cells.

Organophosphate esters (OPEs), used as flame retardants and plasticizers, are present ubiquitously in the environment. Previous studies suggest that exposure to OPEs is detrimental to female fertility in humans. However, no experimental information is available on the effects of OPE mixtures on ovarian granulosa cells, which play essential roles in female reproduction. We used high-content imaging to investigate the effects of environmentally relevant OPE mixtures on KGN human granulosa cell phenotypes. Perturbations to steroidogenesis were assessed using ELISA and qRT-PCR. A high-throughput transcriptomic approach, TempO-Seq, was used to identify transcriptional changes in a targeted panel of genes. Effects on lipid homeostasis were explored using a cholesterol assay and global lipidomic profiling. OPE mixtures altered multiple phenotypic features of KGN cells, with triaryl OPEs in the mixture showing higher potencies than other mixture components. The mixtures increased basal production of steroid hormones; this was mediated by significant changes in the expression of critical transcripts involved in steroidogenesis. Further, the total-OPE mixture disrupted cholesterol homeostasis and the composition of intracellular lipid droplets. Exposure to complex mixtures of OPEs, similar to those found in house dust, may adversely affect female reproductive health by altering a multitude of phenotypic and functional endpoints in granulosa cells. This study provides novel insights into the mechanisms of actions underlying the toxicity induced by OPEs and highlights the need to examine the effects of human relevant chemical mixtures.

Parthenogenetic Stick Insects Exhibit Signatures of Preservation in the Molecular Architecture of Male Reproduction.

After the loss of a trait, theory predicts that the molecular machinery underlying its phenotypic expression should decay. Yet, empirical evidence is contrasting. Here, we test the hypotheses that (i) the molecular ground plan of a lost trait could persist due to pleiotropic effects on other traits and (ii) that gene co-expression network architecture could constrain individual gene expression. Our testing ground has been the Bacillus stick insect species complex, which contains close relatives that are either bisexual or parthenogenetic. After the identification of genes expressed in male reproductive tissues in a bisexual species, we investigated their gene co-expression network structure in two parthenogenetic species. We found that gene co-expression within the male gonads was partially preserved in parthenogens. Furthermore, parthenogens did not show relaxed selection on genes upregulated in male gonads in the bisexual species. As these genes were mostly expressed in female gonads, this preservation could be driven by pleiotropic interactions and an ongoing role in female reproduction. Connectivity within the network also played a key role, with highly connected-and more pleiotropic-genes within male gonad also having a gonad-biased expression in parthenogens. Our findings provide novel insight into the mechanisms which could underlie the production of rare males in parthenogenetic lineages; more generally, they provide an example of the cryptic persistence of a lost trait molecular architecture, driven by gene pleiotropy on other traits and within their co-expression network.

Framing News and Framing Migrants: A Qualitative Study

Frames can have an essential role in the (re)production of the ‘shared ideologies’ concerning several social categories, including migrants. The overall aim of this work is to investigate the discursive construction of frames concerning migration, as rhetoric tools (a) connected to editorial orientations; (b) related to both specific events and more general thematic news articles; (c) having socio-cultural and emotional connotations. The qualitative analysis of 235 Italian news articles enabled us to outline different configurations of discursive elements as well as to identify different emotional pathways. Reflections about the critical role of framing in the social knowledge construction are proposed.

Deep eutectic solvent-based shaking-assisted extraction for determination of bioactive compounds from Norway spruce roots.

Polyphenolic compounds play an essential role in plant growth, reproduction, and defense mechanisms against pathogens and environmental stresses. Extracting these compounds is the initial step in assessing phytochemical changes, where the choice of extraction method significantly influences the extracted analytes. However, due to environmental factors, analyzing numerous samples is necessary for statistically significant results, often leading to the use of harmful organic solvents for extraction. Therefore, in this study, a novel DES-based shaking-assisted extraction procedure for the separation of polyphenolic compounds from plant samples followed by LC-ESI-QTOF-MS analysis was developed. The DES was prepared from choline chloride (ChCl) as the hydrogen bond acceptor (HBA) and fructose (Fru) as the hydrogen bond donor (HBD) at various molar ratios with the addition of 30% water to reduce viscosity. Several experimental variables affecting extraction efficiency were studied and optimized using one-variable-at-a-time (OVAT) and confirmed by response surface design (RS). Nearly the same experimental conditions were obtained using both optimization methods and were set as follows: 30mg of sample, 300mg of ChCl:Fru 1:2 DES containing 30%w/w of water, 500rpm shaking speed, 30min extraction time, 10°C extraction temperature. The results were compared with those obtained using conventional solvents, such as ethanol, methanol and water, whereby the DES-based shaking-assisted extraction method showed a higher efficiency than the classical procedures. The greenness of the developed method was compared with the greenness of existing procedures for the extraction of polyphenolic substances from solid plant samples using the complementary green analytical procedure index (ComplexGAPI) approach, while the results for the developed method were better or comparable to the existing ones. In addition, the practicability of the developed procedure was evaluated by application of the blue applicability grade index (BAGI) metric. The developed procedure was applied to the determination of spruce root samples with satisfactory results and has the potential for use in the analysis of similar plant samples.

Melatonin and its Emerging Physiological Role in Reproduction: A Review and Update.

Melatonin is a neuroendocrine hormone secreted by the pineal gland. The secretion of melatonin follows a circadian rhythm controlled by the suprachiasmatic nucleus, and its secretion is synchronized with the changes in light and dark periods in nature, with the highest secretion level at night. Melatonin is a critical hormone that coordinates external light stimulation and cellular responses of the body. It transmits information about the environmental light cycle, including the circadian and seasonal rhythms, to the relevant tissues and organs in the body, which, along with changes in its secretion level, ensures that its regulated functional activities are adapted in response to changes in the outside environment. Melatonin takes beneficial actions mainly through the interaction with specific membrane-bound receptors, termed MT1 and MT2. Melatonin also acts as a scavenger of free radicals via non-receptor-mediated mechanism. For more than half of acentury melatonin has been associated with vertebrate reproduction, especially in the context of seasonal breeding. Though modern humans show little remaining reproductive seasonality, the relationships between melatonin and human reproduction continue to attract extensive attention. Melatonin plays important roles in improving mitochondrial function, reducing the damage of free radicals, inducing oocyte maturation, increasing fertilization rate and promoting embryonic development, which improves the outcomes of in vitro fertilization and embryo transfer. The present article reviews the progress that has been made in our evolving understanding of the physiological role of melatonin in reproduction and its potential clinical applications in reproductive medicine.