Role Of Cattle Research Articles

The impact of Bluetongue virus on abortion in cattle, sheep and goats in Türkiye: 2012-2017

Bluetongue (BT) is a disease that affects domestic and wild ruminants, and it is caused by a virus called bluetongue virus (BTV) that is transmitted by Culicoides midges. Although clinical signs of BT are most apparent in sheep, BTV could induce abortion and birth defects in cattle, sheep and goats. BTV infection has been reported in Türkiye, but the role of BTV in cattle and small ruminant abortion cases in Türkiye remains uncertain. Therefore, this research aimed to fill this research gap by investigating the prevalence of BTV in cattle and small ruminant abortion cases. To investigate the frequency of BTV in ovine, caprine, and bovine foetuses, a total of 1718 foetuses were collected from different farms between 2012 and 2017. A one-step real-time reverse transcription polymerase chain reaction (RT-PCR) assay was used to detect BTV RNA in aborted foetuses. BTV specific RNA was not detected in the analysed foetuses. To the best of my knowledge, this is the longest study that has investigated whether BTV infection has a role in cattle and small ruminant abortion cases in Türkiye. The findings of the study suggest that BTV infection has no impact on abortion in cattle, sheep and goats in the surveyed regions. The results of this study are limited only to the regions studied. Therefore, further epidemiological studies are needed to confirm the findings of this study.

Molecular, pathological and immunohistochemical investigation of Helicobacteraceae in the abomasum of dairy cattle and sheep in Northern Italy

Although several Helicobacter species have been associated with gastric disease in humans and animals, their role in domestic ruminants is still unclear. This study aimed to investigate the prevalence of H. pylori, “Candidatus H. bovis” and other Helicobacteraceae in the abomasum of dairy cattle and sheep reared in Northern Italy, to shed light on their role as potential reservoir of H. pylori for humans and to evaluate the involvement of Helicobacter spp. in the gastric pathology of these animal species. Abomasa from 25 dairy cows and 24 sheep were collected at slaughter. Following macroscopic examination, samples of fundic and pyloric mucosa were analysed by a screening PCR for Helicobacteraceae, followed by specific PCRs (H. pylori, “Candidatus H. bovis”, and Wolinella spp.), histological examination, and immunohistochemistry for Helicobacter spp. Overall, 96% of the bovine and 42% of the ovine abomasa tested positive for Helicobacteraceae. No samples tested positive for H. pylori, while 68% of bovine samples tested positive for “Candidatus H. bovis”, mostly in the pyloric mucosa. Immunohistochemistry showed colonisation by Helicobacter spp. only in the pyloric glands of 11 bovine abomasa (44%), while in ovine abomasa colonisation was not detected. Our results confirmed that "Candidatus H. bovis" is the most common Helicobacteraceae in the bovine abomasum and specifically colonises bovine pyloric glands. No association was found between Helicobacter spp. infection status and abomasal inflammation, suggesting a high degree of adaptation to the bovine host. The role of cattle and sheep as potential source of H. pylori for humans appears to be negligible.

Gingivitis in cattle and supplemental protein diet: Insights from proteomic analysis

Salivary proteins are essencial in the maintenance of oral homeostasis and can reflect systemic and localized processes, like gingivitis. However, little is known about the relationship between diet and the occurrence of gingivitis in cattle. The present study aimed to characterize the salivary proteomic profile of cattle (n = 12) fed hay (112.19 g/kg of crude protein) cultivated in reformed pastures, and, one group received protein supplement (PS, n = 6); the effect of the protein supplement on the gingival health of the cattle was determined by weekly intraoral examination and periodontal evaluation of the eight (deciduous) incisors. The whole saliva proteome of the two groups was evaluated after 20 and 60 days of confinement. In the periodontal clinical evaluation both groups had episodes of gingivitis; however, the average number of affected sites in the PS group was higher on day 60. The cattle fed exclusively hay, presented a lower average of affected gingival sites on day 60. After 60 days of experimentation, nine biological and 11 immunological processes were altered in bovine saliva. Proteins with multiple functions were detected in the saliva of the cattle; however, differences were observed in their regulation between the two groups. SignificanceIn bovine populations, the relationship between diet and increased incidence of gingivitis is theorized. The results of the present pilot study, both diets caused episodes of gingivitis in the primary dentition of cattle and, apparently, diets with protein supplementation stimulate the expression of salivary proteins with a protective role in cattle that can act against infectious-inflammatory processes, such as gingivitis. However, it is plausible that over time, cattle will adapt to these diets and become more vulnerable to gingivitis.

Beyond nutrition: social–cultural values of meat and meat products among the Borana people of Northern Kenya

AbstractTo most indigenous communities, livestock plays important socio-cultural roles in addition to sustenance. Like many other communities in the world, the Borana people regard livestock and livestock products not only as nourishment but as an integral part of their cultural identity and social construct. The Borana are Cushitic-speaking people who reside in northern Kenya and southern Ethiopia and practise pastoralism as the primary way of livelihood. Livestock is reared by Borana people to obtain meat, milk and other by-products further to socio-cultural roles that were important for communal prosperity and resilience. The aim of this paper was therefore, to describe the various role of cattle and its significance to Borana people. For data collection, qualitative methods such as key informant interviews and focus group discussions were used. The interviews were audio recorded, transcribed and analysed. The results showed that cattle and particularly bulls were slaughtered for meat consumption, rituals and high-value ceremonial purposes. Moreover, as a desired form of wealth, cattle provide numerous resources to the owners and the prestige associated with herd of cattle accelerates social status and identity among the Borana community. Thus, according to participants’ discussions and views, cattle are highly symbolic and have cultural meaning not only as the primary source of food but also in shaping social–cultural values embedded in their social structure. The various role of cattle and its products had significant implications on food security; the traditional practice of livestock wealth sharing shielded the Borana community in times of need and the practice of commensality led to greater social bonds. However, the changing socio-cultural environment caused by recurrent droughts has affected the traditional coping mechanism and led to alternative forms of livelihood.

First Description of Serological Evidence for SARS-CoV-2 in Lactating Cows.

Simple SummarySevere Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) is the agent of the disease that has caused a global pandemic, known as coronavirus disease 2019 (COVID-19). Coronaviruses (CoVs) may emerge from wildlife hosts and infect humans and animals. Up to now, natural infection with SARS-CoV-2 has been reported in several animals, but it has not been found in farm animals, such as buffaloes, goats, sheep, horses, rabbits, hens, pigs, or cows, despite contact with their SARS-CoV-2-positive human breeders. Furthermore, a low susceptibility to SARS-CoV-2 has been detected in experimentally infected cattle with SARS-CoV-2. The unknown zoonotic potential of this virus is a cause of concern for pet owners and farmers. The limited data on cattle suggest that cattle show low susceptibility to SARS-CoV-2 and probably do not function as reservoirs. However, in areas with large cattle populations and a high prevalence of SARS-CoV-2 infection in humans, close contact between livestock and farmworkers may cause reverse zoonotic infections in cattle, as has already been described for highly sensitive animal species, such as minks, cats, and dogs. Thus, studying the zoonotic characteristics of SARS-CoV-2 could help in the development of a strategy for virus detection and the control of viral dissemination.Following the COVID-19 epidemic outbreak in Ariano Irpino, Campania region (Italy), we tested lactating cows for the presence of SARS-CoV-2 on a cattle farm at which, prior to the investigation, 13 of the 20 farmworkers showed COVID-19-like symptoms, and one of them died. Twenty-four lactating cows were sampled to detect SARS-CoV-2. All nasal and rectal swabs and milk samples were negative for SARS-CoV-2 RNA. Of the 24 collected serum samples, 11 showed antibodies against SARS-CoV-2 nucleocapsid protein, 14 showed antibodies against SARS-CoV-2 spike protein, and 13 developed neutralising antibodies for SARS-COV-2; all samples were negative for Bovine Coronavirus (BCoV), another betacoronavirus. To our knowledge, this is the first report of natural serological evidence of SARS-CoV-2 infection in lactating cows. We hypothesise that this may be a case of reverse zoonosis. However, the role of cattle in SARS-CoV-2 infection and transmission seems to be negligible.

Cattle in Buryat Mythology and Ritual

This study addresses, on the basis of ethnographic, folkloric, linguistic, and field data, the role of cattle in Buryat myths and rites, with reference to their economic significance. Buryat words relating to the exteriors of animals, sex differences, etc. are listed. The bull image features in traditional Buryat systems of time calculation and in the tradition of giving protective names homonymical to words denoting the bull are described. Mythological beliefs concerning the cattle are analyzed. The Bulagats, a major Buryat subgroup, practiced the tribal cult of Bukha-noyon, to whom the bull alluded. This practice was connected with the idea of shape-shifting, whereby the bull symbolized the male principle. In terms of cosmogony, the bull was part of habitation spheres such as sky, earth, and water, and their elements such as celestial bodies and mountains, and fire. The positive attitude to the bull and the cow was mirrored by views regarding supernatural properties of bull hair and urine, cow’s milk and placenta, and devices used for managing draft bulls (the yoke and the hair rope zele). At the same time, the cattle were associated with the Lower World and its inhabitants; they functioned as mediators and could symbolize death. A detailed description of the bull image in traditional Buryat ritualism is provided.

Reviewing the ecological evidence base for management of emerging tropical zoonoses: Kyasanur Forest Disease in India as a case study.

Zoonoses disproportionately affect tropical communities and are associated with human modification and use of ecosystems. Effective management is hampered by poor ecological understanding of disease transmission and often focuses on human vaccination or treatment. Better ecological understanding of multi-vector and multi-host transmission, social and environmental factors altering human exposure, might enable a broader suite of management options. Options may include “ecological interventions” that target vectors or hosts and require good knowledge of underlying transmission processes, which may be more effective, economical, and long lasting than conventional approaches. New frameworks identify the hierarchical series of barriers that a pathogen needs to overcome before human spillover occurs and demonstrate how ecological interventions may strengthen these barriers and complement human-focused disease control. We extend these frameworks for vector-borne zoonoses, focusing on Kyasanur Forest Disease Virus (KFDV), a tick-borne, neglected zoonosis affecting poor forest communities in India, involving complex communities of tick and host species. We identify the hierarchical barriers to pathogen transmission targeted by existing management. We show that existing interventions mainly focus on human barriers (via personal protection and vaccination) or at barriers relating to Kyasanur Forest Disease (KFD) vectors (tick control on cattle and at the sites of host (monkey) deaths). We review the validity of existing management guidance for KFD through literature review and interviews with disease managers. Efficacy of interventions was difficult to quantify due to poor empirical understanding of KFDV–vector–host ecology, particularly the role of cattle and monkeys in the disease transmission cycle. Cattle are hypothesised to amplify tick populations. Monkeys may act as sentinels of human infection or are hypothesised to act as amplifying hosts for KFDV, but the spatial scale of risk arising from ticks infected via monkeys versus small mammal reservoirs is unclear. We identified 19 urgent research priorities for refinement of current management strategies or development of ecological interventions targeting vectors and host barriers to prevent disease spillover in the future.

Pemanfaatan Ternak dalam Pengendalian Nyamuk Vektor Penyakit

Mosquito are insects that cause the most health problems in society and animal, namely as nuisance insects and vector of various diseases such as malaria, dengue hemorrhagic fever, chikungunya, Japanese Encephalitis, dirofilariasis, filariasis, St. Louis Encephalitis and West Nile Virus. Control of mosquito as a vector with utilization of cattle (zooprophylaxis) needs to be done so the role of cattle is more optimize. This study aimed to know the effect of the placement of cattle around population to reduce contact between mosquitoes and human. Catching mosquitoes was done at home with two treatments, the house with cattle and house without cattle. Catching mosquitoes in human by bare leg collection method at indoor and outdoor and cattle were placed in a magoon trap. The result showed density of Cx. sitiens are known as vector of Japanese Encephalitis in human at house with cattle 12.05 mosquitoes/man/hour, in human at house without cattle 16.31 mosquitoes/man/hour, and in cattle 54.38 mosquitoes/cattle/hour. Density of Anopheles are known as vector of malaria were caught showed placement of cattle reduce contact between mosquitoes and human. Density of An. sundaicus in human at house with cattle 0.03 mosquitoes/man/hour, in human at house without cattle 0.01 mosquitoes/man/hour, and in cattle 9.63 mosquitoes/cattle/hour. This research showed placement of cattle as zooprophylaxis media around population divert mosquitoes from human to animal

225 Crude protein-content of fat-free muscle and viscera in sheep

Abstract The nutritional effects of variation in feed supply and subsequent compensatory gain can play a significant role in cattle and beef production, due to their effects on carcass quality and feed costs. A system that predicts changes in fat and protein content of muscle and viscera in animals of different life stages and nutritional histories could therefore assist management to optimize performance and reduce costs of feed. A method has been developed to simultaneously estimate body composition and nutrient requirements of ruminants. This method estimates body composition from the difference in energy balance derived from ME intake, and heat production from ME intake and protein content of muscle and viscera, but requires information on protein content of fat free mass in viscera and non-viscera “muscle” tissue. Data from the literature was combined with unpublished data from a study conducted in growing lambs. This experiment tested the effects of ad libitum intake of diets of varying energy density and added RUP on performance and carcass composition of lambs that had been previously restricted or unrestricted prior to a 12–13 week finishing phase, and both carcass and viscera components were chemically analyzed on an individual basis. On a fat-free basis, muscle crude protein averaged 20.8%; this value is in agreement with literature values, which lie between the range of 20–24% and which do not appear to vary substantially with age in postweaning sheep. In the same sheep, crude protein content of viscera averaged 15.6% on a fat-free basis; while data on visceral composition in the literature is limited, these values are within the range of available data. This data is use to parameterize functions describing growth of viscera and changes in heat production over time. This contributes to the method we have developed to estimate nutritional effects on body composition.

Dairy Calves in Uruguay Are Reservoirs of Zoonotic Subtypes of Cryptosporidium parvum and Pose a Potential Risk of Surface Water Contamination.

Cryptosporidium parvum, a major cause of diarrhea in calves, is of concern given its zoonotic potential. Numerous outbreaks of human cryptosporidiosis caused by C. parvum genetic subtypes are reported yearly worldwide, with livestock or water being frequently identified sources of infection. Although cryptosporidiosis has been reported from human patients in Uruguay, particularly children, epidemiologic information is scant and the role of cattle as reservoirs of zoonotic subtypes of C. parvum has not been explored. In this study, we aimed to (a)-identify C. parvum subtypes infecting dairy calves in Uruguay (including potentially zoonotic subtypes), (b)-assess their association with calf diarrhea, (c)-evaluate their spatial clustering, and (d)-assess the distance of infected calves to surface watercourses draining the farmlands and determine whether these watercourses flow into public water treatment plants. Feces of 255 calves that had tested positive for Cryptosporidium spp. by antigen ELISA were selected. Samples had been collected from 29 dairy farms in seven Uruguayan departments where dairy farming is concentrated and represented 170 diarrheic and 85 non-diarrheic calves. Selected samples were processed by nested PCRs targeting the 18S rRNA and gp60 genes followed by sequencing to identify C. parvum subtypes. Of seven C. parvum subtypes detected in 166 calves, five (identified in 143 calves on 28/29 farms) had been identified in humans elsewhere and have zoonotic potential. Subtype IIaA15G2R1 was the most frequent (53.6%; 89/166), followed by IIaA20G1R1 (24.1%; 40/166), IIaA22G1R1 (11.4%; 19/166), IIaA23G1R1 (3.6%; 6/166), IIaA17G2R1 (3%; 5/166), IIaA21G1R1 (2.4%; 4/166), and IIaA16G1R1 (1.8%; 3/166). There were no significant differences in the proportions of diarrheic and non-diarrheic calves infected with any of the C. parvum subtypes. Two spatial clusters were detected, one of which overlapped with Uruguay's capital city and its main water treatment plant (Aguas Corrientes), harvesting surface water to supply ~1,700,000 people. Infected calves on all farms were within 20–900 m of a natural surface watercourse draining the farmland, 10 of which flowed into six water treatment plants located 9–108 km downstream. Four watercourses flowed downstream into Aguas Corrientes. Calves are reservoirs of zoonotic C. parvum subtypes in Uruguay and pose a public health risk.

Cystic Echinococcosis in Algeria: the Role of Cattle as Reservoirs in the Dynamics of Transmission of Echinococcus granulosus to Humans via Dogs.

In North Africa, the parasite Echinococcus granulosus is transmitted in a synanthropic cycle evolving mainly between dogs (DH) and sheep (IH), but other animals like cattle are most often found to be more infested with hydatid cysts but their potential role in human contamination via dogs is unknown. The purpose of this study is to assess the prevalence and fertility rates of hydatid cysts in ruminants (cattle and sheep) in two slaughterhouses in central and eastern Algeria. Determining the frequency and fertility of cysts in cattle will assess the degree of involvement of this species, alongside sheep, in the epidemiological cycle of E. granulosus in Algeria. In the present study, prevalence rates were estimated at 4.9% and 10% in slaughtered ruminants, all species combined at the slaughterhouses of El Harrach and Souk Ahras, respectively. The distribution of the prevalence by species indicates higher infestation rates in cattle compared to sheep: 6% vs 3.9% and 37% vs 4.7% in the slaughterhouses of El Harrach and Souk Ahras, respectively. The survey results showed relatively low cyst fertility rates in cattle compared to sheep: 13.8% vs 43.7% and 33.3% vs 71.4% in the two slaughterhouses, El Harrach and Souk Ahras, respectively. The low fertility rate of cysts in cattle can be explained by a poor adaptation of the species, E. granulosus sensu stricto, previously identified by molecular analysis in all samples of hydatid cysts collected from cattle in Algeria. In conclusion, cattle infested with E. granulosus sensu stricto, with low fertility rates, play a minor role in the epidemiology of cystic echinococcosis in Algeria. It is rather an indicator of the persistence of cystic echinococcosis infection in endemic regions.

О символике связи быка и растений в пехлевийском «Бундахишне»

In the Pahlavi Bundahishn, Ahriman (the supreme deity of evil) has sacrificed the primeval bull beside primordial man Gayomard. Most of the mythical evidence of Indo-European culture illustrates the image or Imagination of the creation of a human or animal sacrifice for the creation of the whole world of its parts and organs. An example of these sacrifices is Gayomard and the primeval bull in Bundashen. In Zoroastrianism, there are more mythical narratives of human beings and less about other phenomena, including animals. However, in the narrative of the creation myth in Iranian culture, besides the creation of parts of the body of the gods or primitive human beings, the archetype of the primeval bull is present. The idea of adaptation of the Macro and Micro worlds in the Iranian narrative refers the distinction between the creation of animals and plants from the body of bull and the manner in which the first pair of creation, Mashya and Mashyana of the body of Gayomard. This is not evident in other narratives of Indo-European culture. This study aims to explain the image of plant creation of organs in primeval bull conjunction with other examples of the creation of the deity or androgynous goddesses and to answer the question of whether the two patterns are essentially interrelated. Therefore, after citing evidence from both instances, it is attempted to analyze this relationship in the above narratives and to show that the role of cattle in the myths of Indo-European creation with the role of goddess in the creation of the world, man, earth, and plant can be co-existent. After bull's death, his body created the plants and animals. This article makes an attempt to explain origin of the animals and vegetable species from the bull-sacrifice based on the sources of myths and archaeological evidences. The bull is the symbol of the fertility goddess, the Earth Goddess, the androgynous being and the origin of life. Therefore, the plants are created from his body.

Reassortment of bluetongue virus vaccine serotypes in cattle.

Bluetongue is primarily a disease of sheep in South Africa, while cattle and goats are mostly subclinically infected. The viraemia of bluetongue virus in cattle lasts much longer than in sheep and the role of cattle in the epidemiology of bluetongue in South Africa is poorly understood. Bluetongue virus has a segmented double-stranded ribonucleic acid genome and reassortment of genomes is a common feature. The aim of the study was to investigate whether reassortment occurs between vaccine and field strains when simultaneously administered to cattle. Six cattle between the ages of 6 and 12 months were infected with five strains of modified live vaccine bluetongue virus and a virulent field isolate of bluetongue virus 4. Blood samples were subsequently collected daily from these animals from day 1 to day 39 post-inoculation. Viruses were directly isolated during viraemia from the buffy coat on Vero cells using the plaque forming unit method. Analysis of plaques indicated that no reassortants between virulent field and vaccine strains occurred and the virulent bluetongue virus 4 was identified as the predominant virus strain. However, a reassortant virus between two bluetongue virus vaccine strains was isolated from the buffy coat. Whole genome sequences from the vaccine viruses were compared to the suspected reassortant and it was found that segment 8 exchanged between the bluetongue virus 8 and bluetongue virus 9 vaccine strains. The use of the live-attenuated bluetongue virus multivalent vaccine in South Africa causes circulation of different vaccine serotypes in Culicoides spp. and susceptible hosts and cattle might provide the ideal host for reassortment to occur.

Reassortment of bluetongue virus vaccine serotypes in cattle

Bluetongue is primarily a disease of sheep in South Africa, while cattle and goats are mostly subclinically infected. The viraemia of bluetongue virus in cattle lasts much longer than in sheep and the role of cattle in the epidemiology of bluetongue in South Africa is poorly understood. Bluetongue virus has a segmented double-stranded ribonucleic acid genome and reassortment of genomes is a common feature. The aim of the study was to investigate whether reassortment occurs between vaccine and field strains when simultaneously administered to cattle. Six cattle between the ages of 6 and 12 months were infected with five strains of modified live vaccine bluetongue virus and a virulent field isolate of bluetongue virus 4. Blood samples were subsequently collected daily from these animals from day 1 to day 39 post-inoculation. Viruses were directly isolated during viraemia from the buffy coat on Vero cells using the plaque forming unit method. Analysis of plaques indicated that no reassortants between virulent field and vaccine strains occurred and the virulent bluetongue virus 4 was identified as the predominant virus strain. However, a reassortant virus between two bluetongue virus vaccine strains was isolated from the buffy coat. Whole genome sequences from the vaccine viruses were compared to the suspected reassortant and it was found that segment 8 exchanged between the bluetongue virus 8 and bluetongue virus 9 vaccine strains. The use of the live-attenuated bluetongue virus multivalent vaccine in South Africa causes circulation of different vaccine serotypes in Culicoides spp. and susceptible hosts and cattle might provide the ideal host for reassortment to occur.

Evidence of increased Hepatitis E virus exposure in Lao villagers with contact to ruminants.

Although pigs are the main reservoir, ruminants have also been shown to be susceptible to hepatitis E virus (HEV). We investigated zoonotic transmission of HEV in rural settings of Lao People's Democratic Republic (Lao PDR) where humans are in close contacts with ruminants and where pigs are rare. Villagers with (n=171, risk group) and without (n=155, control group) cattle were recruited in seven villages in Vientiane Capital. Owners of pigs were excluded. Blood, as well as information on socio-demographics, animal contact, dietary habits and awareness of zoonoses were collected to assess risk factors. Blood and rectal swabs were collected from cattle (n=173) and other ruminants (27 goat, 5 buffaloes) to measure anti-HEV antibody and virus prevalence. A similar anti-HEV antibody seroprevalence was found in cattle (6.8%) and other ruminants (8%). HEV RNA was detected in none of the animal rectal swabs and human sera. Anti-HEV IgG seroprevalence was higher in cattle farmers than in the control group (59.1% vs. 43.9%, p=0.008) and increased significantly with age. Other risk factors included male gender, close contact with cattle and consumption of undercooked meat. We find that HEV is highly endemic in rural Laos and provide first evidence that HEV circulates in free-roaming ruminants with open access to village water sources. Despite some awareness about hygiene, villagers are likely constantly exposed to zoonotic diseases by dietary and lifestyle habits. Cattle farmers had a higher risk of HEV infection than other villagers. Our study highlights the need to raise the awareness of the rural population about water- and food-borne pathogens, and about the role of cattle as a possible source of infection. The knowledge gained on local risk factors and husbandry conditions should guide future awareness raising campaigns and promote appropriate hygienic measures including handwashing and the consumption of safe food and water.

First molecular characterization of Echinococcus granulosus (sensu stricto) genotype 1 among cattle in Sudan

BackgroundEchinococcus granulosus sensu lato (s.l.) is the causative agent of cystic echinococcosis (CE), which is a cosmopolitan zoonotic parasitic disease infecting humans and a wide range of mammalian species including cattle. Currently, little information is available on the genetic diversity of Echinococcus species among livestock in Sudan. In the present study, fifty (n = 50) hydatid cysts were collected from cattle carcasses (one cyst sample per animal) at Al-kadarou slaughterhouse, Khartoum North, Sudan. DNA was extracted from protoscolices and the germinal layer of each cyst and subsequently amplified by PCR targeting the mitochondrial NADH dehydrogenase subunit 1 (NADH-1) gene. The amplified PCR products were purified and subjected to direct sequencing for subsequent construction of phylogenetic tree and net work analysis.ResultsThe phylogenetic tree revealed the presence of Echinococcus canadenesis genotype 6 (G6) in 44 cysts (88.0%), Echinococcus ortleppi genotype 5 (G5) in 4 cysts (8.0%) and Echinococcus granulosus sensu stricto (s.s) genotype 1 (G1) in 2 cysts (4.0%). The phylogenetic network analysis revealed genetic variation among the different haplotypes/genotypes. This report has provided, for the first time, an insight of the role of cattle in the transmission of the zoonotic G1 echinococosis.ConclusionsThe results of the study illustrate that Sudanese breeds of cattle may play an important role in the transmission dynamics and the epidemiology of cystic echinococcosis in Sudan. This study reports the first molecular identification of E. granulosus s.s. in cattle in Central Sudan.

Risk of Shiga Toxigenic Escherichia coli O157:H7 Infection from Raw and Fermented Milk in Sokoto Metropolis, Nigeria

Escherichia coli O157:H7 is an enteric foodborne pathogen associated with life threatening disease conditions. The enterobacteria are frequently found in cattle gastrointestinal tract with high potential of contaminating animal products such as meat, milk, and cheese. A cross-sectional study was conducted to investigate the presence of Shiga toxin-producing Escherichia coli O157:H7 in milk products sold within Sokoto metropolis. Two hundred and sixty (260) samples (comprising 160 raw and 100 fermented milk samples) were collected from different sources within the study area. Bacteriological isolation and biochemical characterization yielded Escherichia coli with a detection rate of 9.23% (24/260). Molecular identification of the recovered isolates by PCR amplification of the Stx1 gene revealed Escherichia coli O157:H7 with a positive rate of 20.83% (5/24). The overall prevalence of E. coli O157:H7 was 1.92% (5/260) and the positive proportions for raw and fermented milk samples were 1.86% (3/160) and 2.0% (2/100), respectively. Fisher's Exact test showed a nonsignificant association between the isolates and the different milk types (p = 0.943; OR = 0.94; [95% CI: 0.154–5.704]). The results revealed presence of Escherichia coli O157:H7 in raw and fermented milk sold within Sokoto metropolis, Nigeria. The findings indicate possible feacal contamination of the milk products, with serious public health consequences. This necessitates the need to screen other milk products produced in the area such as butter and cheese. Health authorities in the State need to enlighten dairy farmers on the zoonotic potential of Escherichia coli O157:H7 and the role of cattle in the spread of the pathogen.

Recombinant β-defensin 126 promotes bull sperm binding to bovine oviductal epithelia.

Primate β-defensin 126 regulates the ability of spermatozoa to bind to oviductal epithelial cells invitro. Bovine β-defensin 126 (BBD126) exhibits preferential expression in the cauda epididymis of the bull, but there have been few studies on its functional role in cattle. The aim of the present study was to examine the role of BBD126 in bull sperm binding to bovine oviductal epithelial cell (BOEC) explants. BBD126 has been shown to be highly resistant to the standard methods of dissociation used in other species and, as a result, corpus epididymal spermatozoa, which have not been exposed to the protein, were used to study the functional role of BBD126. Corpus epididymal spermatozoa were incubated with recombinant (r) BBD126 in the absence or presence of anti-BBD126 antibody. Addition of rBBD126 significantly enhanced the ability of epididymal spermatozoa to bind to BOEC explants (P<0.05). Anti-BBD126 antibody blocked the BBD126-mediated increase in sperm binding capacity. Ejaculated spermatozoa, which are coated with native BBD126 protein but also a large number of seminal plasma proteins invivo, were incubated with rBBD126 in the absence or presence of the anti-BBD126 antibody. Addition of rBBD126 significantly enhanced the ability of ejaculated spermatozoa to bind to BOEC explants (P<0.05), whereas rBBD126 also reduced corpus sperm agglutination (P<0.05). These results suggest that, similar to the role of its analogue in the macaque, spermatozoa with more BBD126 in their acrosome may represent spermatozoa with more oviduct binding capacity.

An investigation about the possible role of cattle and goats as reservoir hosts for Leishmania donovani in Bangladesh.

An investigation about the possible role of cattle and goats as reservoir hosts for Leishmania donovani in Bangladesh.

Радіоекологічна оцінка та прогнозування забруднення овочевої продукції, отриманої у селах центрального лісостепу України радіонуклідами 137Cs і 90Sr: віддалений період після аварії на Чорнобильській АЕС

&lt;p&gt;The radioecological condition of soils in agricultural lands and household plots, with estimated accumulation of &lt;sup&gt;137&lt;/sup&gt;Cs and &lt;sup&gt;90&lt;/sup&gt;Sr in crop and livestock products have been established. We defined the transition ratios of &lt;sup&gt;137&lt;/sup&gt;Cs and &lt;sup&gt;90&lt;/sup&gt;Sr in potatoes and other vegetables grown in household plots by rural dwellers of the Central Forest-Steppe. The role of cattle’s dung in the migration of &lt;sup&gt;137&lt;/sup&gt;Cs and &lt;sup&gt;90&lt;/sup&gt;Sr in radio-contaminated agricultural landscapes and its impact on the level of soil contamination was determined. We suggested that the cattle’s dung obtained from radio-contaminated territories facilitates the migration and redistribution of radionuclides &lt;sup&gt;137&lt;/sup&gt;Cs and &lt;sup&gt;90&lt;/sup&gt;Sr in agricultural landscapes and could be considered as a source of soil secondary pollution.&lt;/p&gt;