Roe Deer Research Articles

Meteorological, demographic, and environmental risk factors for Leishmania infantum in wildlife

Leishmania infantum is an important and neglected vector-borne zoonotic protozoa endemic in the Mediterranean basin. Several wild and domestic mammals can contribute to maintaining its circulation but their importance as effective reservoirs is still under discussion and varies depending on local ecological communities. By combining environmental, climatic, and individual information, this study assessed the presence of L. infantum DNA in a set of wild species from Northwestern Italy and the potential ecological factors related to the risk of infection. From 2020 to 2022, 304 free-ranging wild animals were analyzed for the detection of L. infantum DNA in the spleen and popliteal lymph node (when available).The prevalence obtained in wild boar (Sus scrofa) and roe deer (Capreolus capreolus) was higher than those previously reported (% ± confidence interval 95%; 42.9 ± 18.4% and 27 ± 6.6% in wild boar and roe deer, respectively), and this is the first report of this parasite infecting the coypu Myocastor coypus (60 ± 34.7%). L. infantum DNA was detected in all the seasons including those free of adult sandflies and seasonal differences were minimal, suggesting a long course of infection. The models revealed that animals from rainy areas with higher greenness during the summer, highly populated by humans and predominantly covered by water surfaces had a higher risk of L. infantum.This study contributes to confirming previous findings on the existence of a sylvatic cycle for L. infantum in certain regions of Italy, as well as on the potential epidemiological role of roe deer for this parasite given the elevated prevalence found.

Caesium-137 in the muscles of game animals in 2015-2022 - levels and time trend.

Radioactive caesium-137 occurring in the environment may be taken up by plants and animals and pose a trophic threat to humans. Game animals living in forest ecosystems are very good bioindicators of the level of environmental contamination by ionising radiation. The main species measurably exposed to caesium-137 are the wild boar (Sus scrofa), the roe deer (Capreolus capreolus), and the red deer (Cervus elaphus). The study determined the levels of Cs-137 in muscle samples of these game animals in 2015-2022. Using gamma radiation spectrometry, 858 samples of game animal muscle tissue were examined: 508 wild boar, 145 roe deer and 205 red deer samples. Concentrations of Cs-137 varied widely (from minimum detectable activity (MDA) values to over 4,000 Bq/kg). In 63.4% of cases, the obtained concentrations exceeded the MDA. The permissible limit (600 Bq/kg for food) was exceeded in nine wild boar muscle samples, whereas it was not even exceeded once in roe or red deer muscle. The average concentration in wild boar was three times higher than in roe and red deer and amounted to 42.84 Bq/kg. The highest concentration of Cs-137 in wild boar muscle was 4,195 ± 372.0 Bq/kg, in roe deer muscle it was 111.5 ± 12.50 Bq/kg, and in red deer muscle was 86.70 ± 3.470 Bq/kg. The seven years' data indicate that wild boar absorb the most caesium-137 among game animals. The concentrations of Cs-137 in the muscle of game animals in the years 2015-2022 were at a nearly constant level, a very slow diminution being noticeable over time in roe and red deer muscle.

Helminth Prevalence in European Deer with a Focus on Abomasal Nematodes and the Influence of Livestock Pasture Contact: A Meta-Analysis.

Deer are susceptible to infection with parasitic helminths, including species which are of increasing economic concern to the livestock industry due to anthelmintic drug resistance. This paper systematically collates helminth prevalence data from deer across Europe and explores patterns in relation to host and parasite species, as well as landscape factors. A livestock pasture contact index (LPCI) is developed to predict epidemiological overlap between deer and livestock, and hence to examine deer helminth fauna in the context of their surrounding environment. Fifty-eight studies comprising fallow (Dama dama), red (Cervus elaphus), roe (Capreolus capreolus) and sika (Cervus nippon) deer were identified. Deer populations in "likely" contact with livestock pasture had a higher mean prevalence of the abomasal nematodes Haemonchus contortus, Ostertagia ostertagi, Teladorsagia circumcincta and Trichostrongylus axei (p = 0.01), which are common in livestock and not primarily associated with deer. Roe deer populations had a higher prevalence of T. circumcincta (p = 0.02) and T. axei (p = 0.01) than fallow deer and a higher prevalence of H. contortus than both red (p = 0.01) and fallow deer (p = 0.02). Liver fluke and lungworm species were present sporadically at low prevalence, while the abomasal nematode Ashworthius sidemi occurred locally at high prevalence. Insights from this research suggest that deer helminth fauna is reflective of their surrounding environment, including the livestock species which inhabit areas of shared grazing. This is explored from an epidemiological perspective, and the prospect of helminth transmission between wild and domestic hosts is discussed, including drug-resistant strains, alongside the role of helminths as indicators relevant to the transmission of other pathogens at the wildlife-livestock interface.

Crossings and collisions – Exploring how roe deer navigate the road network

ContextTo investigate the major impact of roads on wildlife, most studies focus on hot-spots of wildlife-vehicle collisions (WVC) to identify areas in need of mitigation measures. However, on road stretches where the frequency of WVC is low, a question arises: is this because those locations are 'safe’ places for wildlife to cross the road with little risk of collisions; or is it because individuals avoid approaching and crossing the road in these locations?ObjectivesIn this study, we addressed this gap by evaluating how roe deer crossings are related to WVC risk across the road network.MethodsWe used 56 076 WVC locations between 2013 and 2017 to predict the spatiotemporal risk zones in response to environmental, road-related and seasonal predictors using Species-Distribution Modelling (SDM). We compared the predictive WVC risk to the location of 20 744 road crossing by 46 GPS-collared roe deer individuals.ResultsWe found that the risk of WVC with roe deer tends to be higher on federal roads that are present in a density of approximate 2.2 km/km2 and surrounded by broad-leafed forests and demonstrate that SDMs can be a powerful tool to predict the risk of WVC across the road network. Roe deer crossed roads more frequently in high WVC risk areas. Temporally, the number of WVC changed throughout the year, which can be linked to roe deer movement patterns rather than landscape features. Within this study, we did not identify any road segments that were a complete barrier to roe deer movement.ConclusionsThe absence of complete barriers to roe deer movement detected in the present study, might be due to the low spatial variation of the landscape, coupled with the high individual variation in movement behaviour. By applying our approach at greater spatial scales and in other landscape contexts, future studies can continue to explore the potential barrier impacts of roads on landscape connectivity. Exploring the relationship between crossing activity and collision risk can improve one’s ability to correctly identify road stretches that require mitigation measures to improve connectivity versus reduce collisions.

Roe Deer, Lithuania’s Smallest and Most Abundant Cervid

A review of 113 scientific and other publications on the smallest and most abundant deer in Lithuania, the roe deer (Capreolus capreolus), is presented, along with an analysis based on compound annual population growth rates of population numbers, hunting bags, and roadkill. This review covers the species’ history in the country from the last glaciation onwards, the changes in numbers from 1934 to 2023, hunting bag changes from 1965 to 2022, roadkill numbers from 2002 to 2022, data on roe deer reproduction, habitat selection, genetic diversity, pathogens, and damage to forest stands. It also provides an overview of species management and selection for trophies. Despite the exponential increase in roe deer numbers since 1990 and the dominance of this species in roadkill, even on urban roads, the number and density of animals and the damage they cause to forest stands are relatively low compared to other European countries. Within the observed period, drops in numbers were related to harsh winters in 1969/1970 and 1995/1996. Poaching, especially coinciding with weakened enforcement during the period of political–economic transition in the early 1990s, has also had a negative impact on population numbers, as have recent increases in the number of large predators. Population growth over the recent period does not correlate with hunting mortality, which has remained stable at an average of 16.9% since 2002.

Dissecting the Effects of Cephenemyia stimulator on the Olfactory Turbinates and Nasopharynx of Roe Deers (Capreolus capreolus).

Nasopharyngeal myiasis in European roe deer (Capreolus capreolus) is a pathological condition caused by the larval stages of Cephenemyia stimulator, a fly from the Oestridae family. These larvae reside in the host's upper respiratory tract for months, inducing significant tissue damage and clinical symptoms. The lifecycle of Cephenemyia stimulator is complex, involving three larval stages before maturation into adult flies, with each stage contributing to the progressive pathology observed in the host. Despite their prevalence, the histopathological effects of these larvae in the nasal and nasopharyngeal cavities have been understudied. Our study fills this knowledge gap by providing a detailed histopathological analysis of the affected tissues, using various staining techniques to reveal the extent and nature of the damage caused by these parasitic larvae. This histopathological examination reveals significant alterations within the nasopharyngeal mucosa and nasal cavity, including erythematous changes, mucosal metaplasia, fibrosis, and tissue necrosis. Parasitic cysts and eosinophilic infiltration further characterize the impact of the infestation, compromising not only the mucosal integrity but also potentially the olfactory function of the affected animals. This research is crucial for understanding the impact of myiasis on both the health and olfactory capabilities of roe deer populations and could have significant implications for wildlife management and conservation.

Same landscape, different connectivity: contrasting patterns of gene flow in two sympatric ungulates in a mountain area

Comparative landscape genetics studies provide insights on the impact of landscape elements on gene flow patterns of different species inhabiting the same geographic area. We investigated the population genetic structure of two sympatric ungulates, roe deer Capreolus capreolus and Northern chamois Rupicapra rupicapra, in a mountain area of the central Italian Alps (Trentino, northern Italy). A total of 122 chamois and 72 roe deer samples were genotyped by two species-specific panels of 11 polymorphic microsatellite loci and analyzed by aspatial and spatially explicit analyses. While the roe deer population resulted unstructured, a clear population structure was detected in chamois, with two main groups, one inhabiting the eastern and the other spread in the western part of the study area. Landscape genetics analysis confirmed these scenarios and revealed a different effect of landscape on gene flow. An IBD (Isolation-By-Distance) model best explained genetic variation in roe deer, while IBR (Isolation-By-Resistance) was found as the process underlying genetic variation patterns in chamois, suggesting arable lands, coniferous forests, watercourses, and main roads as potential barriers. Species distribution and landscape use might explain these results: roe deer mostly occupy valley floors relatively connected to each other, and their spatial behavior may promote gene flow across areas. On the other hand, chamois prefer higher elevations and their movements may be hindered by valleys, rivers, and road networks. This study highlights the different impacts of natural and anthropic landscape elements on gene flow in two sympatric species, resulting from their different ecological requirements.

Molecular and Serological Detection of Bovine Coronaviruses in Marmots (Marmota marmota) in the Alpine Region.

In this study, virological surveillance focused on coronaviruses in marmots in the Alpine region in 2022, captured as part of a population control reduction program in the Livigno area. Seventy-six faecal samples were randomly collected from marmots at the time of capture and release and tested for genome detection of pan-coronavirus, pan-pestivirus, canine distemper virus, and influenza A and D virus. Nine faecal samples were positive in the Pan-CoV RT-PCR, while all were negative for the other viruses. Pan-coronavirus positives were further identified using Illumina's complete genome sequencing, which showed the highest homology with Bovine Coronavirus previously detected in roe deer in the Alps. Blood samples (n.35) were collected randomly from animals at release and tested for bovine coronavirus (BCoV) antibodies using competitive ELISA and VNT. Serological analyses revealed that 8/35 sera were positive for BCoV antibodies in both serological tests. This study provides molecular and serological evidence of the presence of BCoV in an alpine marmot population due to a likely spillover event. Marmots share areas and pastures with roe deer and other wild ruminants, and environmental transmission is a concrete possibility.

Risk response towards roads is consistent across multiple species in a temperate forest ecosystem

Roads can have diverse impacts on wildlife species, and while some species may adapt effectively, others may not. Studying multiple species' responses to the same infrastructure in a given area can help understand this variation and reveal the effects of disturbance on the ecology of wildlife communities. This study investigates the behavioural responses of four species with distinctive ecological and behavioural traits to roads in the protected Bohemian Forest Ecosystem in central Europe: European roe deer Capreolus capreolus, a solitary herbivore; red deer Cervus elaphus a gregarious herbivore; wild boar Sus scrofa, a gregarious omnivore and Eurasian lynx Lynx lynx, a solitary large carnivore. We used GPS data gathered from each species to study movement behaviour and habitat selection in relation to roads using an integrated step selection analysis. For all species and sexes, we predicted increased movement rates in response to roads, selection of vegetation cover near roads and open areas after road crossings, and increased road avoidance during the day. We found remarkably similar behavioural responses towards roads across species. The behavioural adaptations to road exposure, such as increased movement rates and selection for vegetation cover, were analogous to responses to natural predation risk. Roads were more strongly avoided during daytime, when traffic volume was high. Road crossings were more frequent at twilight and at night within open areas offering food resources. Gregarious animals exposed to roads favoured stronger road avoidance over faster movements. Ungulates crossed roads more at twilight, coinciding with commuter traffic during winter. Despite differences in the ecology and behaviour of the four species, our results showed similar adaptations towards a common threat. The continuous expansion of the global transportation network should be accompanied by efforts to understand and minimise the impact of roads on wildlife to assist wildlife management and ensure conservation.

Not Just Scraping By: Experimental Evidence for Large Cutting Tools in the High Lodge Non-handaxe Industry

The presence or absence of handaxes endures as the major criterion of Lower Palaeolithic classification, with contemporaneous core-and-flake industries modelled as simpler counterparts to Acheulean technology. This is based on the supposed absence of formal tools, particularly of large cutting tools (LCTs) which are understood to be important within Acheulean lifeways, functioning as butchery knives among other uses. Scrapers from the core-and-flake industry of High Lodge (MIS 13) evidence formalised flake-tool production techniques, geared towards large tools with long cutting edges and acute angles, comparable in many respects to Acheulean handaxes. A holistic set of experiments was designed to test the production, efficiency, and practical utility of these scrapers. The experiments compared these scraper forms against handaxes and Quina scrapers. Their use in roe deer butchery indicates functional differences but demonstrates the appropriacy of both large, refined scrapers, and handaxes for processing carcasses of this size. The results support the inclusion of High Lodge scraper forms within the standard definition of LCTs. This interpretation challenges perceived discrepancies between handaxe and non-handaxe industries and deterministic explanations for Acheulean material culture. The feasibility of alternative LCTs supports the argument that the Acheulean represents socially inherited behaviours rather than latent reinventions.

Taking the Deer by the Antlers: Deer in Material Culture in the Balkan Neolithic

Prehistoric communities had strong ties with the animal world that surrounded them—animals were prey, sources of food, and raw materials, but also threats and mysteries, and certain animals often had an important place in the symbolic realm. With the process of domestication and the switch to animal husbandry as the main source of animal food, these relations changed considerably, and a certain dichotomy between “the domestic” and “the wild” may be noted in numerous past communities. When it comes to the Neolithic period in the Balkans, domestic animals had an important place in subsistence and economy, and it seems that cattle had a particularly prominent symbolic role. Wild species preserved some of their significance in both subsistence and symbolic realms, especially cervids (red deer, roe deer, and fallow deer). In this paper, the place of deer in the material culture of the Neolithic communities in the Balkans will be analysed: skeletal elements of deer were used for the production of diverse items, including non-utilitarian ones, or were part of ritual depositions, and deer representations are encountered in other materials, such as clay figurines. The symbolic meaning of deer cannot be reconstructed with certainty; however, it is probable that deer were tied with territoriality and the landscape.

PCR-based study on viral pathogens circulation among cervids in the Moscow region

A molecular survey of selected viruses in free-ranging cervids was conducted in 15 different districts of Moscow region. Samples were collected from 178 game animals including 144 moose (Alces alces), 19 roe deer (Capreolus capreolus) and 15 deer without species information. Nasal swabs and tissue samples including parts of the nasal septum, upper tracheal rings, lung, heart, liver, kidneys and pooled organ samples were tested using polymerase chain reaction (PCR). Samples were studied for pestiviruses, herpesviruses, coronaviruses, group A rotaviruses, adenoviruses, hepatitis e and parainfluenza type 3 virus. None of the samples were positive for Bovine Coronavirus and SARS-COV-2, hepatitis E virus and parainfluenza type 3 virus. PCR results were positive for bovine herpesviruses (5.05%), pestiviruses (0.56%), rotaviruses (1.68%). DNA of a new adenovirus, presumably causing a mild course of animal respiratory disease, was detected in samples of 6 animals (3.37%). In conclusion, the conducted studies have shown that game animals of the Moscow region can be a natural reservoir of cattle viruses, and this must be taken into account when planning and organizing measures for the control and eradication of such notifiable diseases as bovine viral diarrhoea and infectious bovine rhinotracheitis. Monitoring studies and general disease surveillance of wild animal populations provide additional information on the epidemiology of infectious diseases in the region and allow timely measures to be taken to protect wild animals, domestic animals and the public.

Molecular analysis of scats revealed diet and prey choice of grey wolves and Eurasian lynx in the contact zone between the Dinaric Mountains and the Alps

A comprehensive understanding of the dietary habits of carnivores is essential to get ecological insights into their role in the ecosystem, potential competition with other carnivorous species, and their effect on prey populations. Genetic analysis of non-invasive samples, such as scats, can supplement behavioural or microscopic diet investigations. The objective of this study was to employ DNA metabarcoding to accurately determine the prey species in grey wolf (Canis lupus) and Eurasian lynx (Lynx lynx) scat samples collected in the Julian Alps and the Dinaric Mountains, Slovenia. The primary prey of wolves were red deer (Cervus elaphus) (detected in 96% scat samples), European roe deer (Capreolus capreolus) (68%), and wild boar (Sus scrofa) (45%). A smaller portion of their diet consisted of mesocarnivores, small mammals, and domestic animals. In contrast, the lynx diet mostly consisted of European roe deer (82%) and red deer (64%). However, small mammals and domestic animals were also present in lynx diet, albeit to a lesser extent. Our findings indicate that the dietary habits of wolves and lynx are influenced by geographical location. Snapshot dietary analyses using metabarcoding are valuable for comprehending the behaviour and ecology of predators, and for devising conservation measures aimed at sustainable management of both their natural habitats and prey populations. However, to gain a more detailed understanding of wolf and lynx dietary habits and ecological impact, it would be essential to conduct long-term genetic monitoring of their diet.

New data on the mammoth fauna in the Amga River basin

Many unique finds of Pleistocene animals have been made on the territory of Yakutia, including whole frozen bodies. However, the majority of the finds come from the northern regions. On the territory of Central Yakutia, in contrast to Northern Yakutia, there are much fewer finds. This is due to the smaller number of preserved relict ice deposits of the Upper Pleistocene ("Yedoma") and the lack of systematic, licensed collection of mammoth ivory and other remains of the mammoth fauna due to their unsatisfactory preservation, which is caused by the stronger thawing of the permafrost in summer in Central Yakutia. There are relatively few published scientific papers on the mammoth fauna of the Amgar River basin. Therefore, new data from this area are of scientific interest. We have studied new material on the mammoth fauna from the "Mount Korolenko" locality, located on the left bank of the Amgar River near the village of the same name. The material was collected during school expeditions organised by the Amga Lyceum and the Russian Geographical Society in 2022-2023. It shows that at this site bone remains are washed out by the river on the right bank of the Amgas. Scattered bones were found on both the left and right banks, but the right bank is richer in palaeontological material. The bone remains are represented by fragments and whole bones of the postcranial skeleton of various animals. The age of most of the finds probably corresponds to the Karga interstadial. The taxonomic identification of the bones showed the presence of the following species: woolly mammoth, woolly rhinoceros, Lena horse, steppe bison, reindeer, red deer. There is a fragment of the humerus of a roe deer, possibly of late Neopleistocene age. A morphometric study of the bone remains was carried out and comparisons made with literature data. The list of large mammals that lived in the Amgas basin in the late Neoproterozoic has been completed.

Food security and the value of game animals—a study of Sweden

The food security value of wild meat is calculated by combining proxy methods for quantifying game animal abundance with shadow pricing techniques for assessing the unit values of food security. This study calculated the food security values of moose, roe deer, wild boar, and fallow deer for Sweden overall and for individual counties. The results showed that meat from these animal populations accounts for approximately 9% of meat consumption in Sweden and for 1.2% of the minimum energy food consumption during periods of crisis for the whole of Sweden, while in some counties it can be as much as 8%. The calculated unit value, or shadow price, of the minimum energy requirement ranged between € 0.1 and € 4.2/mcal, depending on the magnitude of the crisis scenario. At most, the total food security value of actual animal population sizes amounted to 0.50 billion euros, but this was unevenly distributed, with high values in counties that have an abundance of moose and wild boar.

Red fox cannibalism in a temperate forest ecosystem

Scavengers benefit from carrion and simultaneously provide essential ecosystem services. To assess benefits and risks that carrion might bring, it is crucial to understand ecosystem-specific scavenger communities. Carrion research has mostly focussed on ungulate carcasses and has rarely explored the effects of carnivore carcasses, which can be critical to understanding disease transmission pathways. Therefore, using red fox, roe deer and red deer carcasses, we investigated factors that affect facultative vertebrate scavenger assemblages visiting carnivore and herbivore carcasses in a temperate forest ecosystem. Due to potentially increased disease transmission risk associated with carnivore carrion, we predicted that carnivore carcasses would be visited less and have a reduced visiting species composition compared to herbivore carcasses. Further, we expected red foxes to exhibit less consumption behavior of conspecific carcasses. We placed 22 red fox and 22 herbivore carcasses in a temperate montane protected area in Central Europe, the Bavarian Forest National Park, and analyzed the visits of all species and the consumption behavior of red fox at carcasses to understand whether these depended on carcass type. We found no significant effects of carcass type on visitation rates, visiting species composition, or red fox behavior. Cannibalism of foxes was common and, when controlling for confounding factors, e.g., elevation, temperature, and scavenging seasonality, the predicted consumption rate of red fox at conspecifics was higher than at herbivore carrion. Foxes appeared to consume conspecifics earlier than herbivore carrion, but the difference was not significant. At the very least, our results show that carnivore carcasses and fox behavior, including cannibalism, could provide a pathway for disease transmission. We thereby provide a first impression of the patterns of vertebrate scavenger assemblages at different carcass types in a temperate ecosystem and show that red fox behavior might precipitate a disease transmission pathway.

Taphonomy of the fauna and chert assemblages from the Middle Palaeolithic site of Vajo Salsone, Eastern Italian Alps

The Vajo Salsone site is located at an altitude of 376m a.s.l. on the right slope of the Valpantena valley, in the Veneto Pre-Alps, north of the town of Verona. The site was discovered in 2017 during the construction of a truckable road. It is a small and narrow karst cavity filled with Pleistocene sediments that yield abundant Middle Palaeolithic artefacts and faunal remains, often coated by carbonate encrustations. The lithic assemblage shows the predominance of the Levallois method and is characterised by a large number of retouched elements, including foliated points. These artefacts, typical of the Late Middle Palaeolithic period in central and south-eastern Europe, make Vajo Salsone an exceptional case, as their recovery is rather sporadic west of the Balkan Peninsula and south of the Alps. The fauna is dominated by ungulates, the most abundant being the red deer followed by roe deer, chamois and elk. Among the carnivores, wolf is common, while fox and bear are rare. This fauna suggests an environment characterised by open glades and closed forests, with a discontinuous presence of alpine grasslands and wetlands. Taphonomic analysis of the bone surfaces is complicated by the presence of concretions on most of the remains, which also show a high degree of fragmentation. Post-depositional fractures are common, and weathering is light. Only cervid bones show anthropogenic modifications related to hunting and hominin exploitation such as cut marks, scraping, impacts and burnt modifications. Some long bone diaphysis bear marks testifying to the use of these fragments as retouchers for flint artefacts. Such use has been evidenced at other Middle Palaeolithic sites in the area. Furthermore, the good state of preservation of the occlusal surface enamel of the cervid teeth allowed us to carry out meso and microwear analyses which provided data on seasonality and site occupation. With regard to dental wear analysis, the red deer from Vajo Salsone had mesowear suggesting a mixed feeding and grazing diets, whereas microwear analysis showed that the individuals had a diet between mixed feeding and browsing. The taphonomic study of the site has been integrated by X–RFS analysis of the alterations occurred to the lithic artefacts, which include deep patination and the formation of calcareous crusts. All the data presented here are consistent with the hypothesis of a short-distance displacement of the material from a single archaeological deposit possibly related to human occupation during the warm season.

Vulnerability and fascination with wildlife encounters and psychological restoration in local natural settings

ABSTRACT People often visit natural settings for recreation and psychological restoration. This study aims to improve understanding of how exposure to and experiences of mammalian wildlife in local natural settings can permit and promote recreation and restoration. Randomly sampled residents in three regions of Sweden (N = 303) with varying presence of fear-relevant species (wolf, wild boar) and fear-irrelevant species (roe deer, squirrel) completed a questionnaire. Squirrel and roe deer were rated significantly higher than wolf and wild boar for anticipated positive feelings and restoration outcomes, and significantly lower for negative feelings and avoidance of natural settings. The possibility of exposure explained little of the variation in the restoration variables, whereas the experiences anticipated with an animal encounter contributed substantially to explanation. Vulnerability seems to counteract the restorative benefit of fascination. Wildlife conservation efforts and public health initiatives may find practical utility in distinguishing between potential exposure and anticipated experience when designing interventions.

Nematode fauna of the digestive tract of Siberian roe deer in Primorsky Krai

Nematode fauna of the digestive tract of Siberian roe deer in Primorsky Krai

Developmental progression continues during embryonic diapause in the roe deer

Embryonic diapause in mammals is a temporary developmental delay occurring at the blastocyst stage. In contrast to other diapausing species displaying a full arrest, the blastocyst of the European roe deer (Capreolus capreolus) proliferates continuously and displays considerable morphological changes in the inner cell mass. We hypothesised that developmental progression also continues during this period. Here we evaluate the mRNA abundance of developmental marker genes in embryos during diapause and elongation. Our results show that morphological rearrangements of the epiblast during diapause correlate with gene expression patterns and changes in cell polarity. Immunohistochemical staining further supports these findings. Primitive endoderm formation occurs during diapause in embryos composed of around 3,000 cells. Gastrulation coincides with elongation and thus takes place after embryo reactivation. The slow developmental progression makes the roe deer an interesting model for unravelling the link between proliferation and differentiation and requirements for embryo survival.