Mastering the vibration characteristics of PDC bit, especially in interbedded formations, can help to explore reasonable bit failure control measures, achieve bit optimization and drilling optimization. This work used single rocks to construct different types of vertical and horizontal interbedded rocks with different interbedded properties, the experimental study obtained the bit vibration characteristics under single rock, sudden-changed rock, and alternating rock conditions. The vibration acceleration of a single rock is consistent with the rock strength. The acceleration increases with the increase of weight-on-bit and rotary speed, the influence of weight-on-bit is greater than that of rotary speed. The maximum amplitude of accelerations always occurs in the low frequency range of 0∼300 Hz, the difference between rocks is mainly reflected in the high frequency range greater than 400 Hz. When the drill bit suddenly crosses from soft rock to hard rock in the vertical interbedded formation, the acceleration increases rapidly, causing direct impact. When the drill bit suddenly crosses from hard rock to soft rock, the impact and acceleration attenuates slowly, which could cause fatigue damage to the drill bit and induce high-frequency vibration. The rapid and significant change in depth-of-cut is the fundamental reason for the impact generated when the bit crosses through the interlayer, and both the maximum and minimum depth-of-cut occur in the transition region. PDC bit alternately encountered soft and hard rocks in the horizontal interbedded formation, causing intensified vibration. The acceleration is consistent with rock difference. The influence of weight-on-bit on acceleration increases with the increase of rotary speed for horizontal interbedded formation with small rock differences. However, with the increase of weight-on-bit, the influence trend and degree of rotary speed on acceleration remain basically unchanged. The increase in weight-on-bit /rotary speed weakens the effect of rotary speed/ weight-on-bit on acceleration in horizontal interbedded formations with significant rock differences. The research results illustrate that the reason for the abnormal bit failure in interbedded formations is the frequent changes in depth of cut caused by rock mutation and alternation, and the resulting impact. This provides theoretical guidance for the implementation of effective measures such as reasonable cutter arrangement design, pertinent setting of auxiliary structures, and appropriate adjustment of drilling parameters.