Robusta Coffee Beans Research Articles

Characterization of Pectinase Enzyme Produced from Indigenous Yeast Isolates During Fermentation of Robusta Coffee Beans

The following research aims to understand indigenous yeast isolates that are pectinolytic, the effect of incubation time on pectinase enzyme activity, and the pH and temperature properties of pectinase enzymes obtained from indigenous yeasts from spontaneous fermentation of robusta coffee beans. The following type of research is an experiment through RAL (Completely Randomized Design). The results showed that testing of seven indigenous yeast isolates was pectinolytic and that the highest pectinolytic ability was isolate K24I7 with a clear zone diameter of 3.13 mm. The characterization results of the pectinase enzyme produced from isolate K24I7 were optimum at pH 5.5 with an enzyme specific activity value of 10.09 U/mg and optimum temperature in the range of 40oC-60oC with an average enzyme specific activity of 10.08 U/mg. The pectinase enzyme produced from isolate K24I7 is thermophile because it is still active at 70°C

Caffeine removal and compositions losses from whole Robusta coffee beans during conventional and ultrasound-assisted aqueous decaffeination

Whole Robusta coffee beans were decaffeinated using conventional aqueous extraction (CAE) and ultrasound-assisted aqueous extraction (UAAE). Changing of extraction water at time intervals to enhance caffeine removal efficiency is proposed as novel decaffeination technique. Kinetic modelling of caffeine content evolutions during CAE and UAAE, with and without water changing, was performed to confirm benefit of newly proposed technique. Losses of soluble carbohydrates, phenolics, sugars, flavonoids and organic acids from decaffeinated beans were investigated. Recycling of extraction water as way to recover lost compositions was tested. Water changing enhanced caffeine removal efficiency, especially in case of UAAE; caffeine could be removed by 98%. Kinetic model well described caffeine content evolutions (R2 = 0.99). This strategy nevertheless led to significant losses (p < 0.05) of bean compositions when compared to CAE. UAAE with water recycling could not help recover bean compositions, but drying and soaking beans in spent extraction water from second round of decaffeination significantly increased (p < 0.05) total soluble carbohydrates and phenolics contents.

Test of Level of Likes on Fermentation Length and Physical Quality Characteristics of Robusta Coffee After Storage

One of the stages in the wet processing of coffee that can affect its flavor is fermentation. Fermentation is the process of removing the mucilage layer that still adheres to the coffee beans, which results in a distinctive coffee aroma and reduces caffeine content, especially in robusta coffee. After the fermentation process, packaging and storage are carried out. Storage is the stage where raw coffee beans are kept to maintain their quality until the next process. This study aims to determine the effect of fermentation duration on panelists' preference levels and the impact of storage duration on the physical quality characteristics of robusta coffee. This research employs both quantitative and qualitative descriptive methods. The results show that storage duration does not affect the physical quality characteristics of robusta coffee beans. The moisture content parameter after storage was found to be 12.2%, the density parameter averaged 0.691 gr/ml, and the defect value parameter fell into grade 3. The study also shows that different fermentation durations do not significantly affect the panelists' preference levels, with an average score of 3-4,1.

Impact of Seasonal Weather Variations on Physical Attributes of Robusta Coffee Beans

Impact of Seasonal Weather Variations on Physical Attributes of Robusta Coffee Beans

Energy Analysis and Economy Performance of a Hybrid Solar Dryer for Drying Coffee

This work studies the effect of the drying temperature on the profile of the water content, drying rate, drying efficiency, economic performance, and the quality of Robusta and Arabica coffee beans using the hybrid solar dryer. The drying instrument with the help of LPG heating is used in this research with a specified temperature of 40 ℃, 50 ℃, and 60 ℃. The research is conducted on a sunny day for 1 day for each temperature and coffee beans. The temperature profile shows that the lowest water content in the study is 60 ℃. The drying rate for both 50 ℃ and 60 ℃ are similar and the highest average efficiency of the instrument is at 50 ℃ for both Robusta and Arabica beans. The dominant peak in GCMS analysis result of coffee samples was caffeine with a total area percentage of 30.89%, the description of the coffee bean structure using SEM test resulted in a hole size of 5-10 µm, the obtained fat content was 1.6%, the obtained protein content 17.3%. A hybrid solar dryer is an environmentally friendly solution that enables faster coffee drying, with a payback period of 1.5 years for both coffee bean types.

Immunoexpression of aortic endothelial P-selectin and serum apolipoprotein A-1 levels after administration of arabica (Coffea arabica) and robusta (Coffea canephora) coffee bean extracts: In vivo study in atherosclerosis rat model.

Atherosclerosis is a leading cause of cardiovascular disease-related death worldwide. Some studies suggested that the natural ingredients in coffee may negatively affect cardiovascular diseases, while other studies indicated that coffee contains anti-inflammatory compounds that are beneficial for cardiovascular diseases. The aim of this study was to measure the expression of P-selectin in aortic endothelial cells and the level of serum apolipoprotein A-1 (ApoA-1) in an atherosclerosis rat model after the administration of arabica and robusta coffee bean extracts at mild-moderate and high doses. An experimental study was conducted with a complete randomized design using 36 adult male white rats (Rattus norvegicus) divided into six groups: negative control (NC), positive control (PC), arabica mild-moderate dose (A1), arabica high dose (A2), robusta mild-moderate dose (R1), and robusta high dose (R2). Animals were induced atherosclerosis with atherogenic feed and then were treated with arabica and robusta coffee bean extracts at two different doses for four weeks. The results showed that the expression of P-selectin in the group of rats treated with robusta coffee bean extract was lower than arabica coffee bean extract group. Rats with robusta coffee bean extract mild-moderate dose had the highest ApoA-1 levels compared to other groups significantly (p<0.05). The level of ApoA-1 was higher in both mild-moderate and high dose of robusta coffee groups compared to the negative control group (both with p<0.001). In conclusion, mild-moderate intake of robusta coffee bean extract could reduce aortic P-selectin immunoexpression and increase serum ApoA-1 levels in an atherosclerosis rat model.

Perbedaan Metode Pengolahan Pascapanen dalam Memengaruhi Kadar Kafein Roasted Bean Kopi Robusta Argopuro

This study aims to determine the caffeine content of Argopuro Jember robusta coffee beans based on differences in post-harvest processing methods. The research was conducted at the Jember State Polytechnic Agricultural Products Processing (PHP) Laboratory and the Jember State University Integrated Testing Unit Laboratory in June-September 2022. The samples were robusta coffee logs originating from the Argopuro area of ​​Jember. The post-harvest coffee processing process is divided into four (4) methods, namely natural processing (dry process), semi-wet processing, wet (full wash process), and honey processing. Green beans from the results of each processing method are roasted at light, medium, and dark levels. The roasted bean caffeine content test from each post-harvest processing method at each roasting level was carried out descriptively (simple, without repetition). Overall, the caffeine content of the roasted beans produced in this study still meets the SNI 01-3542-2004 standard, a maximum of 2%. The caffeine content of Argopuro Jember robusta coffee roast beans at the light roast level ranges from 1.44-1.65%, 1.38-1.92% for medium roast, and 1.61-1.81% for dark roast. The highest caffeine content in roasted beans at each roasting level is 1.65% (dry process), 1.92% (full wash process), and 1.81% (full wash process), respectively. Meanwhile, roasted beans with the lowest caffeine content with a value of 1.38% are produced using the semi-wet process processing method at a medium roast level. The fluctuations in caffeine values ​​produced in this study indicate that post-harvest processing methods do not affect the caffeine content of roasted beans at light, medium, or dark roast levels.

Mycotoxin contamination profiling in coffee bean by targeted LC-HRMS

Abstract Indonesia is the fourth coffee producer in the world which is 95% produced by smallholder plantations with various ways of processing and storage in the form of green beans and roasted beans. This study aims to analyse the presence of mycotoxin contamination of Menoreh coffee in various storage levels using Liquid Chromatography tandem High-Resolution Mass Spectrometry (LC-HRMS) analysis. The analysis of mycotoxin contamination in green beans and roasted beans is crucial to ensure the safety and quality of the coffee for consumption. This study used a random sampling method. The samples tested were Menoreh Robusta coffee bean from two processors in Samigaluh, Kulon Progo, Yogyakarta, Indonesia. The results showed that green beans stored in the dryer house for more than 6 months were contaminated with aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), ochratoxin, and patulin with 24.48; 10.98; 57.18 and 16.92 of area max (106), respectively. Samples of broken green beans stored in contaminated warehouses were contaminated with patulin. However, all roasted bean and roasted bean samples that have been mashed do not contain aflatoxin B1, B2, or patulin. Targeted metabolomic using LC-HRMS profiling is a powerful tool for the rapid detection of mycotoxin in coffee bean. This method could be developed for quantitative analysis to provide accurate concentration.

Influence of drying condition on the drying constants and activation energy for robusta coffee using heat pump drying

Abstract Heat pump drying (HPD) is a method of drying agricultural products which has great potential due to its high efficiency and energy savings. Coffee is a heat sensitive product, HPD was chosen as an alternative to existing drying methods for coffee processing. Understanding of the HPD process is important to know for optimizing the coffee drying process with HPD especially in drying characteristics. The purpose of this research is to determine the number for the drying rate constant and the energy of activation in coffee drying. The material used in this study was wet parchment Robusta coffee beans (Coffea canephora). The research was carried out experimentally for 5 h at 700 lpm air flow with varying drying air conditions by adjusting the drying temperature and specific humidity. The drying temperatures used were 60, 65, 70, 75, and 80 °C. The drying temperature is limited to 80 °C based on SNI 7467-2008. Variations in specific humidity are determined based on temperature settings in the refrigeration system of 10, 15, 20 °C and without a refrigeration system. The maximum drying rate constant was 10.69×10-3 s-1, achieved at a temperature of drying variation of 80 °C while a specific humidity of 6.16 g H2O/kg dry air, while the minimum activation energy value was 23.43 kJ/mol at the same specific humidity. The lowest drying rate constant value was 5.79×10-3 s-1, achieved at a temperature of drying variation of 60 °C and a specific humidity of 17.24 g H2O/kg dry air, while the greatest activation energy level was 26.18 kJ/mol at the same specific humidity.

Physicochemical and sensory analysis of mangrove seed coffee (Sonneratia ovata) compare with robusta coffee from Indonesian origin

Coffee is a global drink for millennials. Indonesia is one of the countries with the most significant coffee consumption in the world. The main problem with coffee consumption is the caffeine content, many researchers are looking for alternative coffee drinks that do not contain caffeine, one of which is mangrove fruit seeds. Caffeine can be removed during roasting, but over-roasting produces unwanted sensory impacts on coffee. This study aimed to determine mangrove coffee beans' sensory characteristics and caffeine content to compare with robusta coffee as commercial coffee. In addition, it is also necessary to monitor changes in the properties of the mixture of Robusta coffee beans and mangrove seeds during the roasting process in real-time. The method used was a randomized block design, using two factors, commercial coffee (C) and mangrove seed coffee (M), and 3 test levels, light (L) at 193°C to 199°C, medium (M) at 204°C and dark roast (D) at 213°C to 221°C. Analysis of data using Analysis of variance (ANOVA). Significantly different test results were then tested using Duncan's advanced test. Data processing was carried out using SPSS Version 28 software. The results showed that the sensory characteristics of commercial coffee (Robusta) were preferable to mangrove coffee, but the impact of caffeine could be eliminated by consuming mangrove coffee. The degree of roasting greatly affects coffee's sensory and chemical quality, with the best results obtained in commercial coffee roasted at a medium level (M) at 204°C. The comparison results showed that the characteristics of coffee between commercial coffee (Robusta) and mangrove coffee have phenol content, total acid, and proximate and sensory values close to commercial coffee. However, the caffeine content is not found in mangrove coffee, so coffee connoisseurs can use mangrove coffee because it can be used as an alternative to coffee drinks without giving the effect of caffeine addiction.

Effect of Different Drying Techniques on Quality Attributes of Ready to Reconstitute (RTR) Coffee Mix

Coffee is one of the most ubiquitous and culturally significant beverages worldwide, transcending borders and influencing societies in myriad ways. Ready-to-reconstitute (RTR) coffee, often referred to as instant coffee, occupies a unique and significant niche in the global coffee market. In the present study, A mixture of Arabica to Robusta coffee beans in the ratio of 70:30 was found most suitable for manufacture of ready to reconstitute coffee mix. The concentrated coffee decoction prepared using this mixture is to be blended with concentrated milk (30%TS) in the ratio of 1:5 and dried using vacuum tray dryer, tray dryer and freeze dryer. The coffee mix is vacuum-dried at 30 to 45 degrees Celsius with a vacuum of 600 to 650 mm of Hg, gives better quality of dried product. The optimized final product has better rheological and physico chemical characteristics. In present study found that ready to reconstitute coffee mix has solubility in hot water 26.4 seconds and 2.74 minutes in cold water at 16◦C. The freeze-dried coffee mix powder exhibited reduced flowability 42.08 seconds compare to vacuum try dried and tray-dried coffee mix power that was 47.41 and 46.27 seconds respectively. Same as bulk density of freeze-dried powder (0.64 g/cm3) found lowest compare to vacuum tray dried (0.78 g/cm3) and tray dried (0.82 g/cm3) coffee mix powder.

Chemical characteristics of original robusta coffee beans from Jember fermented with Saccharomyces cerevisiae starter using the semi-carbonic maceration technique

Abstract This research aims to evaluate the chemical characteristics of original robusta coffee beans fermented using Saccharomyces cerevisiae. The method used is semi-carbonic maceration with variations in starter concentration (20%, 30%) and fermentation times (24 hours, 48 hours, 72 hours). The results of the research showed that samples with 30% concentration of starter and 72 hours fermentation times showed a decrease in chemical compound content with the lowest values, including caffeine content of 0.91%, reducing sugar content of 0.86%, and protein content of 10.79%. On the other hand, control samples without fermentation treatment had the highest values for each chemical compound content. The pH value obtained ranges from 4.97 to 4.47 and has a water content of between 10.20% - 11.37% and based on SNI. This research shows that fermentation with Saccharomyces cerevisiae can have an influence on the quality of original robusta coffee beans and is expected to provide good taste and aroma.

Defects of Coffee Beans with Different Postharvest Processes and Roasting Temperatures on Volatile Compounds of Coffee Beans from Coffee Small-Scale Industries of West Lampung Indonesia

Specialty coffee is processed by performing high standards nationally (SNI) and internationally (SCAA). Good handling in the small coffee industry is essential to producing coffee raw materials as a refreshing drink. The aroma released from the best coffee beans gives an emotional flavor to the drinker - the aroma produced from the best coffee beans with different post-harvest methods. This study analyzed the physical quality of moisture content, coffee bean defects from dry processing post-harvest handling (natural and honey), and different roasting temperature differences to produce volatile compound profiles from three small-scale coffee industries. Six samples of raw materials from 3 small-scale coffee industries were selected by purposive sampling with the criteria that the processing farmer had performed SNI standards. Six coffee samples from the September harvest in 2022. Observations were made in the laboratory for analysis of moisture content and quality defects of coffee beans, then roasted by the industry and analyzed descriptively and multivariate analysis for volatile compounds. The moisture content of coffee beans from all coffee industries has met the requirements following SNI 01-2907-2008, that the moisture content is not more than 12.5 %. All coffee beans produced have 50 % potential as fine robusta coffee beans because they have no primary defects. Increasing the roasting temperature on all coffee beans increases volatile compounds. Coffee beans processed with honey produced more volatile compounds than the natural post-harvest method. The caffeine content in all post-harvest processing processes in the three industries has a difference in concentration due to differences in roasting temperature and time. The study that in the three-ground coffee small-scale industries processed by the natural post-harvest method, carbamic acid compounds were suspected to be from carbamate class insect pesticides (carbofuran) and the fungicide pyraclostrobin. Considering that pesticide residues harm human health, it is essential to implement measures to educate farmers to use organic fertilizers and ensure consumer safety. HIGHLIGHTS Kopi merupakan komoditas unggulan Provinsi Lampung Indonesia Kopi cacat mempengaruhi Kualitas mutu biji kopi Kopi yang dihasilkan IKM merupakan Kopi robusta petik buah merah Terdapat temuan kontaminasi dari senyawa kimia carbamate acid dari pemakaian herbisida/insektisida yang digunakan petani GRAPHICAL ABSTRACT

Microbiological, Phytochemical Constituents, and Antioxidant Properties of Fermented Green Robusta Coffee Beans

Robusta coffee is one of Malaysia’s most planted species due to its ability to adapt to the local climate. Nonetheless, the coffee species was perceived as having lower quality and economic value due to bitterness and astringency. It is widely believed that higher caffeine and chlorogenic acid contents in Robusta coffee beans contributed to the unfavourable bitter and astringent flavour. Hence, the present study intends to evaluate the effect of spontaneous wet fermentation (SWF) of locally grown Robusta (&lt;i&gt;Coffea canephora&lt;/i&gt; L.) coffee towards the microbiological properties, phytochemical constituents, in particular caffeine and chlorogenic acids (CGA), total phenolic content (TPC), and antioxidant properties. The SWF of green Robusta coffee beans from University Agricultural Park (UAP), Universiti Putra Malaysia, Serdang, Selangor, took place at ambient temperatures between 25 to 28°C, and the pH decreased from 5.2 to 3.64 over five days of fermentation. The total plate count, lactic acid bacteria (LAB) and yeasts were significantly increased to approximately 7 Log10 CFU/g. The SWF has reduced caffeine content by 35%, while the CGA has decreased by roughly 20%. The SWF also led to an increase in TPC of approximately 31.5% and an increase in antioxidant activity of approximately 60%.

Coffee Type Classification Using Backpropagation Artificial Neural Network

Coffee has several types including robusta coffee, arabica coffee and luwak coffee. Each coffee has certain characteristics of color, texture, aroma and also the quality of the taste. Coffee counterfeiting is also common. This coffee counterfeiting usually uses materials such as corn, wheat, soybeans, husks, sticks and robusta coffee beans. So that a model is needed to be able to classify the type of coffee. This research uses artificial neural network machine learning algorithms to identify and classify coffee. Quality training and testing data is needed in this method because it will affect the final results. Initial data is collected via e-nose, with this equipment data on changes in electrical voltage will be obtained from 4 sensors, namely MQ-2, MQ-3, MQ-7 and MQ-135. These 4 features will be used in the classification process. With 900 sets of training data, the test results show that the neural network is able to provide correct classification 99% of the 3 sets of testing data. The results of training and testing show that the neural network formed can identify and distinguish coffee types with good results.

Daya Hambat Pasta Gigi yang Mengandung Ekstrak Biji Kopi Robusta (Coffea canephora) Terhadap Pertumbuhan Bakteri Aggregatibacter actinomycetemcomitans Secara In Vitro

Periodontitis is caused by bacteria in plaque, namely Aggregatibacter actinomycetemcomitans. Prevention of periodontal disease can be done by controlling plaque, namely brushing teeth using toothpaste to remove plaque and prevent plaque maturation. Alternative ingredients (herbs) that can be used to make toothpaste are robusta coffee beans which contain caffeine, flavonoids, trigonelin, and chlorogenic acid as antibacterial. The purpose of this study was to analyze the inhibition of toothpaste containing robusta coffee bean extract at concentrations of 6.25%, 12.5%, 25%, and 50% against the growth of A. actinomycetemcomitans. This type of research is experimental laboratory with posttest only control group design. This study was divided into 6 groups, namely positive control (Arabica coffee toothpaste), negative control (placebo paste), Robusta coffee bean extract toothpaste 6.25%, 12.5%, 25%, and 50%. Inhibition test using well diffusion method by making holes in the media. The diameter of the inhibition zone was measured using a caliper. The results showed that the control (-) group and the 6.25% group had no inhibitory power against the growth of A. actinomycetemcomitans. The robusta coffee bean extract toothpaste at concentrations of the 50% group (18.69 mm) had the greatest inhibition, but K (+) had greater inhibition than the 50% (22.89 mm) group. The conclusion of this study is that the robusta coffee bean extract toothpaste at concentrations of 12.5%, 25%, and 50% had inhibitory power against the growth of A. actinomycetemcomitans, but the greatest inhibitory power was at a concentration of 50%.

Perbandingan Variasi Lamanya Waktu Penyangraian Terhadap Kadar Kafein Pada Biji Kopi Robusta Secara Spektrofotometri UV-Vis

Robutsta coffee is one of the most popular beverages in the world is consumed by many people. The main compound in coffee beans is caffeine. This compound affects the central nervous system, muscles, and kidneys. Caffeine cluster has kromoform, so it can be read using its absorbance of UV-Vis spectrophotometry. This research aims to know the level of caffeine in coffee beans with variation of roasting time duration. The determination of the levels of caffeine by using quantitative analysis, by way of determination of wavelength maximum, operating time, raw curve, and the determination of the levels of caffeine on the sample with variations of roasting time. Determination of the wavelength, the parent solution taken 10 ml, 50 ml measuring flask put in added HCI 0,1 N up to the mark with a boundary. The next assigment that is the determination of the levels of caffeine in samples. Each sample taken as many 3 ml filtrate into a 50 ml measuring flask, then added HCI 0,1 N up to the mark with a boundary. The result obtained in this research is the maximum wavelength obtained 274,0 nm. The sample content of raw robusta coffee beans is an average of 1,77% and robusta coffee bean content with variations of roasted time of 10 minutes is an average of 1,62 %, roasted time of 15 minutes is an average of 1,31 %, and roaster time of 21 minutes is an average of 1,10 %. The duration of the roasting test indicates that is a significant difference in caffeine content.

Quantitative Analysis of Phytochemical Compounds and Antihyperglycemic Potential of Robusta Coffee from West Lampung

West Lampung Regency in Lampung Province produces high quality robusta coffee with a distinct, strong bitter flavour. Bitter flavour indicates the amount of bioactive substances produced by plants called phytochemicals. The aim of this study is to analyse the phytochemical compounds and antihyperglycemic potential of robusta coffee beans from West Lampung, Indonesia. Quantitative phytochemical analysis was carried out using the thin layer chromatography method and spectrophotometry. Animal experimental design using robusta coffee on hyperglycemia conditions in mice which was induced by alloxan 170 mg/kgBW subcutaneously and given by 1 ml of brewed robusta coffee for 10 days. The robusta coffee from West Lampung contained total caffeine 4,014.87 µg/g, total flavonoid content (TFC) 93.6 mg quercetin equivalent per gram of sample, with total alkaloid content (TAC) 0.848 mg of quinine equivalent per gram of sample, total tannin content (TTC) 182.3 mg of tannic acid equivalent per gram of sample, and total saponin content (TSC) 24.2 mg of quillaja bark equivalent per gram of sample. The administration of 1 ml/mice/day of robusta coffee brewed for 10 days did not show a decrease in blood sugar level in hyperglycemic mice, due to the short duration of the study, so the role of coffee in decreasing hyperglycemia conditions has not been optimally observed.

Climate change impacts on coffee production in Indonesia: A review

Indonesia is ranked in the top 5 coffee production countries in the world with the largest production being Arabica and Robusta coffee beans. Approximately ninety percent of coffee produced in Indonesia is being exported and as the trend in local consumption increases, the demand for coffee is escalating. However, future coffee production is challenged by climate change. Background: This paper outlines the impacts of climate change variables including environmental stressors on coffee production quality and quantity of Arabica and Robusta species, discusses the influences on suitable areas for coffee cultivation and the coffee pests related to coffee productivity, as well as suggests mitigation and adaptation approaches in Indonesia. Methods: The literature review approach was utilized to complete this paper which involves collecting, analyzing, and correlating the findings of prior studies. The paper only considered literature with the following terms in the title; climate change impact, coffee production, mitigation, adaptation, and Indonesia. Finding: Increasing temperature degrades coffee production both in Arabica and Robusta coffee beans. The overall suitable area for cultivating coffee is shifting towards higher altitudes However, Indonesia is projected to lose more than half of the suitable area for cultivating Arabica by the year 2050. Climate change improves coffee pests' thermal tolerance and enables them to grow in warm temperatures. Conclusion: Common mitigation approaches include shade plantation and soil moisturization control which both improve coffee productivity. Another strategy is the cultivation calendar modified the coffee life cycle with climate prediction.

Green Robusta Coffee Bean Extract (GRCBE) inhibits bone loss in wistar rat models of Lps P. gingivalis and NiTi wire-induced experimental periodontitis

Oral exposure to alloys frequently leads to negative reactions, both in the immediate area and across the body. The usage of coffee extracts is anticipated to enhance cellular function by counteracting the effects of pro-inflammatory signals that are involved in chronic inflammatory responses triggered by inflammatory agents like bacteria and metal ions. The objective is to develop a new approach using an extract obtained from Robusta coffee beans as an anti-inflammatory substance, which may have therapeutic benefits in preventing bone loss in Periodontitis Rat. The main cause is primarily attributed to the existence of metals and LPS P. gingivalis in the oral cavity. Methods. A randomized controlled trial was conducted, enroling 35 male Wistar rats that were divided into 7 groups and used as the experimental animals. Groups 1–4 consisted of rats that did not receive any therapy, while groups 5–7 comprised rats that were treated with GRCBE. The rats were subjected to decapitation after 14 days. The Green Robusta Coffee bean extract (GRCBE) was derived from the unprocessed green beans of Javanese Robusta coffee. We discovered in prior experimental experiments that GRCBE at a concentration of 500 mg/ml can inhibit the progression of periodontitis in rats. The substances used to cause inflammation and immune system impairment were NiTi wire and LPS P. gingivalis. Immunohistochemistry was used to assess the expressions of OCN, BMP2, and COX-2. Results. The simultaneous exposure of P. gingivalis lipopolysaccharide (LPS) and NiTi wire in rats led to an upregulation of OCN and BMP2 expression, while the expression of COX-2 reduced. The administration of GRCBE resulted in a steady drop in pro-inflammatory biomarkers, specifically OCN and BMP2, while the anti-inflammatory biomarker COX2 rose. In conclusion. In the field of dentistry, the coexistence of P. gingivalis LPS and metallic biomaterials can influence the occurrence of alveolar bone resorption in a rat model of periodontitis. GRCBE exhibits potential as an innovative approach for reducing periodontitis induced by the simultaneous activation of metal and LPS P. gingivalis. In this setting, it has the potential to be employed as an anti-inflammatory remedy.