A robust trajectory optimization approach for guidance algorithm gain and target vector selection for powered descent and landing is developed. A genetic algorithm is used to determine optimized guidance algorithm parameters to minimize the impact of initial condition, environment, navigation, and vehicle property uncertainty on flight performance for a given sensor suite. Vehicle state uncertainties are computed efficiently using linear covariance analysis techniques. When implemented in the guidance algorithm, the optimized gains and target vectors shape a trajectory that has more favorable conditions for a given navigation sensor suite, resulting in improved flight performance. As a demonstration of this method, the optimized guidance parameters are found for a multiphase trajectory from powered descent initiation to touchdown for a robotic lunar landing mission. Single-objective optimization results demonstrate a reduction in uncertainty and an improvement in nominal performance. Multi-objective optimization results showing the tradeoff between terminal position uncertainty and total propellant usage are presented for multiple sensor suite compositions. Further, guidance parameters selected using the developed robust trajectory design approach may enable acceptable flight performance with fewer and/or lower-quality sensors. The resulting Pareto fronts present the optimal trade space early in the mission design process to enable informed decision-making.