Rivers In Norway Research Articles

Determination Procedure of Ecotoxicologically Acceptable Cu Concentration in River Considering the Change of Environmental Factors

Objectives : In this study, considering the changes in the concentration of metal/metalloid and environmental factors over time of the target water system and the toxic effects of metals on various aquatic organisms, determined procedure of ecotoxicologically acceptable metal/metalloid concentration based on a biotic ligand model (BLM) was proposed in detail. As an application example, the ecotoxicologically acceptable Cu concentration for the Numedalslågen river in Norway was calculated.Methods : The Cu concentration and environmental factor time series data of the target water system were collected, and EC50[Cu]<sub>T</sub>, the total concentration of Cu in the water system showing a toxic effect of 50% on the target aquatic organisms, was calculated through BLM. In addition, the predicted no-effect concentration (PNEC) was obtained from the species sensitivity distribution using the acute-chronic ratio and adjustment factor, and the ecotoxicologically acceptable Cu concentration of the target water system was determined through the fixed monitoring benchmark (FMB).Results and Discussion : Ecotoxicologically acceptable Cu concentration of the target water system was determined as 1.15 μg/L. Dissolved organic carbon (DOC) showed the highest linear relationship with EC50[Cu]<sub>T</sub> (R<sup>2</sup> = 0.94-0.98) compared to various environmental factors, and the Cu toxicity effect on aquatic organisms tended to decrease as the DOC concentration increased. Ca showed a low linear relationship with EC50[Cu]<sub>T</sub> (R<sup>2</sup> < 0.04), which is presumed to be due to the decrease in Ca<sup>2+</sup> concentration due to the complexese formation of DOC and Ca<sup>2+</sup>, which reduced the competitive effect with Cu<sup>2+</sup>.Conclusion : The proposed procedure provides a useful method for calculating ecotoxicologically acceptable metal concentrations for the protection of aquatic organisms in water systems. In addition, it is expected that it can be used to calculate site-specific metal concentrations that can protect aquatic organisms in the target water system by reflecting the timing of sediment resuspension, which can cause rapid changes in the concentration of metals and environmental factors in the water system, and specific time points such as storm and flood seasons.

Automated fluvial hydromorphology mapping from airborne remote sensing

AbstractMapping fluvial hydromorphology is an important part of defining river habitat. Mapping via field sampling or hydraulic modeling is however time consuming, and mapping hydromorphology directly from remote sensing data may offer an efficient solution. Here, we present a system for automated classification of fluvial hydromorphology based on a deep learning classification scheme applied to aerial orthophotos. Using selected rivers in Norway, we show how surface flow patterns (smooth or rippled surfaces vs. standing waves) can be classified in imagery using a trained convolutional neural network (achieving a training and validation accuracy of >95%). We show how integration of these classified surface flow patterns with information on channel gradient, obtained from airborne topographic LiDAR data, can be used to compartmentalize the rivers into hydromorphological units (HMUs) that represent the dominant flow features. Automated classifications were broadly consistent with manual classifications that had been made in previous ground surveys, with equivalency in automated and manually derived HMU classes ranging from 61.5% to 87.7%, depending on the river stretch considered. They were found to be discharge‐dependent, showing the temporally dynamic aspect of hydromorphology. The proposed system is quick, flexible, generalizable, and provides consistent classifications free from interpretation bias. The deep learning approach used here can be customized to provide more detailed information on flow features, such as delineating between standing waves and advective diffusion of air bubbles/foam, to provide a more refined classification of surface flow patterns, and the classification approach can be further advanced by inclusion of additional remote sensing methods that provide further information on hydromorphological features.

Quantitative Evaluation of Bathymetric LiDAR Sensors and Acquisition Approaches in Lærdal River in Norway

The development of bathymetric LiDAR technology has contributed significantly to both the quality and quantity of river bathymetry data. Although several bathymetric LiDAR sensors are available today, studies that evaluate the performance of the different bathymetric LiDAR sensors comparatively are still lacking. This study evaluates the performance of three bathymetric LiDAR sensors, CZMIL Supernova, Riegl VQ880-G, and Riegl VQ840-G, used with different acquisition approaches, in mapping Lærdal River bathymetry in Norway. The performance was evaluated based on comparing the sensors against a multibeam echosounder (MBES), a terrestrial laser scanner (TLS), and by an intercomparison between the individual sensors. The comparison was completed by comparing point clouds from the instruments and through the comparison of DEMs created from the point clouds. For the comparison against the MBES, the results show that the median residuals range between 3 to 13 cm, while against the TLS the median residuals range between 0 to 5 cm. The comparison of the CZMIL sensor against the two Riegl sensors shows median residuals of around 12 cm where the CZMIL map is shallower against the VQ880-G and deeper against the VQ840-G sensor. For the two Riegl sensors, the results show a median difference of 2 cm with the VQ880-G map deeper. We do observe that areas with high residuals are linked to river features such as large substrate variability, steep banks, and whitewater/turbulent flow. The study shows that all the LiDAR instruments provide high-quality representations of the river geometry and create a solid foundation for planning, modelling, or other work in rivers where detailed bathymetry is needed.

Capture-Recapture Models with Heterogeneous Temporary Emigration

ABSTRACT We propose a novel approach for modeling capture-recapture (CR) data on open populations that exhibit temporary emigration, while also accounting for individual heterogeneity to allow for differences in visit patterns and capture probabilities between individuals. Our modeling approach combines changepoint processes—fitted using an adaptive approach—for inferring individual visits, with Bayesian mixture modeling—fitted using a nonparametric approach—for identifying clusters of individuals with similar visit patterns or capture probabilities. The proposed method is extremely flexible as it can be applied to any CR dataset and is not reliant upon specialized sampling schemes, such as Pollock’s robust design. We fit the new model to motivating data on salmon anglers collected annually at the Gaula river in Norway. Our results when analyzing data from the 2017, 2018, and 2019 seasons reveal two clusters of anglers—consistent across years—with substantially different visit patterns. Most anglers are allocated to the “occasional visitors” cluster, making infrequent and shorter visits with mean total length of stay at the river of around seven days, whereas there also exists a small cluster of “super visitors,” with regular and longer visits, with mean total length of stay of around 30 days in a season. Our estimate of the probability of catching salmon whilst at the river is more than three times higher than that obtained when using a model that does not account for temporary emigration, giving us a better understanding of the impact of fishing at the river. Finally, we discuss the effect of the COVID-19 pandemic on the angling population by modeling data from the 2020 season. Supplementary materials for this article are available online.

Short-term effects of macrophyte removal on aquatic biodiversity in rivers and lakes

Mass development of macrophytes is an increasing problem in many aquatic systems worldwide. Dense mats of macrophytes can negatively affect activities like boating, fishing or hydropower production and one of the management measures often applied is mechanical removal. In this study, we analyzed the effect of mechanical macrophyte removal on phytoplankton, zooplankton, and macroinvertebrate (pelagic and benthic samples) assemblages. Our study covered five sites in four countries in Europe and Africa with highly variable characteristics. In all sites, dense mats of different macrophyte species (Juncus bulbosus in a river in Norway; a mix of native macrophytes in a German river, Elodea nuttallii in a lake in Germany, Ludwigia spp. In a French lake and Pontederia crassipes in a South African lake) are problematic and mechanical removal was applied. In every country, we repeated the same BACI (Before-After-Control-Impact) design, including “before”, “one week after”, and “six weeks after” sampling in a control and an impact section. Repeating the same experimental design at all sites allowed us to disentangle common effects across all sites from site-specific effects. For each taxonomic group, we analyzed three structural and three functional parameters, which we combined in a scoring system. Overall, the removal of macrophytes negatively affected biodiversity, in particular of zooplankton and macroinvertebrate assemblages. In contrast, plant removal had positive effects on the phytoplankton assemblages. Effects were more pronounced one week after removal than six weeks after. Consequently, we suggest a stronger consideration of the effect of plant removal on biodiversity to arrive at more sustainable management practices in the future.

Ecological status assessment of clay rivers with naturally enhanced water phosphorus concentrations

• Water P and the PIT index were higher in unimpacted clay than non-clay rivers. • Water TP was less bioavailable in unimpacted clay rivers than in non-clay rivers. • Natural water TP increases with catchment clay cover. • The PIT good/moderate class boundary increases with catchment clay cover. The species composition of benthic algae changes as water phosphorus concentrations increase, and these changes can be used for ecological status assessment according to the Water Framework Directive. Natural background phosphorus concentrations in rivers and streams that are unaffected by anthropogenic impacts are usually low. Running waters draining catchments with deposits of marine clay, however, may have enhanced phosphorus concentrations, because the clay is naturally rich in apatite. Almost all clay rich areas have been cultivated for centuries, however, and fertilization has increased the soil phosphorus levels. It has, therefore, been difficult to disentangle natural from anthropogenically enhanced phosphorus in streams draining clay rich areas. We compared water phosphorus concentrations, and the Periphyton Index of Trophic Status PIT, between clay and non-clay, impacted and unimpacted rivers in Norway. We found that water phosphorus concentrations and the PIT index were higher in unimpacted clay rivers than in unimpacted non-clay rivers, indicating that natural phosphorus concentrations in clay rivers are indeed enhanced compared to rivers without deposits of marine clay. In addition, phosphate-P contributed 18–23% to total phosphorus in unimpacted clay rivers, but 33–37% in unimpacted and impacted non-clay rivers and clay rivers affected by agriculture. This indicates that the total phosphorus in unimpacted clay rivers is less bioavailable than in non-clay rivers and in impacted clay rivers. Water total phosphorus concentrations in unimpacted clay rivers significantly increased with catchment clay cover. Based on these findings, we derived new status class boundaries for the PIT index in clay rivers. Clay rivers are suggested to be assessed in only two status classes, i.e., “good or better” or “moderate or worse”, respectively. The good/moderate status class boundary for the PIT index was shown to increase with increasing catchment clay cover. .

Achieving sustainable low flow using hydropower reservoir for ecological water management in Glomma River Norway

Generally, low flow in rivers occur as a result of extended period of dryness which is likely attributed to drought. Drought is a natural occurrence as an outcome of reduction in precipitation in a region for a long time. Hence, low flow is a growing concern as there are possibilities of more reduced flows in rivers. However, reservoirs can be utilized to mitigate negative effects on the supply of water in dry periods and supply water for other purposes. This study aims at verifying how the low flow condition of Glomma River in Norway has been progressively sustained by hydropower reservoirs. Water Evaluation and Planning Systems (WEAP) software was used for modelling the natural streamflow condition of Glomma River, which is the longest river in Norway, using two unregulated sub-basins within the Glomma catchment. Findings from this study presents that the period between January and March are critical periods in Glomma River. Results show that the values the annual minimum low flow gotten from the three gauges suggest the flow after regulation has increased significantly as opposed to before regulation. The daily average flow is simulated by WEAP to be an average of 100 m3/s during the low flow periods and an average discharge of 350 m3/s during the summer. However, the result indicates that the flow in the summer has reduced by 80% in majority of the years. In addition, Nash Sutcliffe efficiency (NSE) for the two sub basins used for this study was 0.9 and 0.76, respectively. Also, the calculation of the coefficient of determination (R2) resulted in 0.85 and 0.78 respectively for the two sub basins. In particular, findings from this study presents evidence on the low flow condition in Glomma River prior to its regulation and how the regulation has sustained the flow.

Classification of hydropeaking impacts on Atlantic salmon populations in regulated rivers

AbstractThis article proposes and demonstrates a new classification system of fish population level effects of hydropeaking operations in rivers. The classification of impacts is developed along two axes; first, the hydromorphological effect axis assesses the ecohydraulic alterations in rivers introduced by rapid and frequent variations in flow and water level, second the vulnerability axis assesses the site‐specific vulnerability of the fish population. Finally, the population level impact is classified into four classes from small to very large by combining the two axes. The system was tested in four rivers in Norway exposed to hydropeaking, and they displayed a range of outcomes from small to very large impacts on the salmon populations. The river with a relatively high base flow and ramping restrictions scored better than rivers with the lower base flow or limited ramping restrictions, indicating that hydropeaking effects can be mitigated while maintaining high hydropower flexibility. Most effect factors could easily be calculated from timeseries of discharge and water level, whereas the use of hydraulic models to estimate potential stranding areas may require more work. The vulnerability factors are mainly qualitative and depend more heavily on expert judgments and are thus more uncertain. The system was deemed suitable for the purpose of supporting management decisions for rivers exposed to hydropeaking operations. It evaluates the severity of the additional pressures due to hydropeaking operations and proved useful to identify mitigating measures. While the system was developed for Atlantic salmon river systems, it could be adapted to other species or systems.

Characterization of the atmospheric environment during extreme precipitation events associated with atmospheric rivers in Norway - Seasonal and regional aspects

Extreme precipitation events in Norway in all seasons are often linked to atmospheric rivers (AR). We show that during the period 1979–2018 78.5% of the daily extreme precipitation events in Southwestern Norway are linked to ARs, this percentage decreasing to 59% in the more northern coastal regions and ~40% in the inland regions. The association of extreme precipitation with AR occurs most often in fall for the coastal areas and in summer inland. All Norwegian regions experience stronger winds and 1–2°C increase of the temperature at 850 hPa during AR events compared to the climatology, the extreme precipitation largely contributing to the wet climatology (only considering rainy days) in Norway but also in Denmark and Sweden when the rest of Europe is dry. A cyclone is found nearby the AR landfall point in 70% of the cases. When the cyclone is located over the British Isles, as it is typically the case when ARs reach Southeastern Norway, it is associated with cyclonic Rossby wave breaking whereas when the ARs reach more northern regions, anticyclonic wave breaking occurs over Northern Europe. Cyclone-centered composites show that the mean sea level pressure is not significantly different between the eight Norwegian regions, that baroclinic interaction can still take place although the cyclone is close to its decay phase and that the maximum precipitation occurs ahead of the AR. Lagrangian air parcel tracking shows that moisture uptake mainly occurs over the North Atlantic for the coastal regions with an additional source over Europe for the more eastern and inland regions.

New flood frequency estimates for the largest river in Norway based on the combination of short and long time series

Abstract. The Glomma River is the largest in Norway, with a catchment area of 154 450 km2. People living near the shores of this river are frequently exposed to destructive floods that impair local cities and communities. Unfortunately, design flood predictions are hampered by uncertainty since the standard flood records are much shorter than the requested return period and the climate is also expected to change in the coming decades. Here we combine systematic historical and paleo information in an effort to improve flood frequency analysis and better understand potential linkages to both climate and non-climatic forcing. Specifically, we (i) compile historical flood data from the existing literature, (ii) produce high-resolution X-ray fluorescence (XRF), magnetic susceptibility (MS), and computed tomography (CT) scanning data from a sediment core covering the last 10 300 years, and (iii) integrate these data sets in order to better estimate design floods and assess non-stationarities. Based on observations from Lake Flyginnsjøen, receiving sediments from Glomma only when it reaches a certain threshold, we can estimate flood frequency in a moving window of 50 years across millennia revealing that past flood frequency is non-stationary on different timescales. We observe that periods with increased flood activity (4000–2000 years ago and <1000 years ago) correspond broadly to intervals with lower than average summer temperatures and glacier growth, whereas intervals with higher than average summer temperatures and receding glaciers overlap with periods of reduced numbers of floods (10 000 to 4000 years ago and 2200 to 1000 years ago). The flood frequency shows significant non-stationarities within periods with increased flood activity, as was the case for the 18th century, including the 1789 CE (“Stor-Ofsen”) flood, the largest on record for the last 10 300 years at this site. Using the identified non-stationarities in the paleoflood record allowed us to estimate non-stationary design floods. In particular, we found that the design flood was 23 % higher during the 18th century than today and that long-term trends in flood variability are intrinsically linked to the availability of snow in late spring linking climate change to adjustments in flood frequency.

International Regulations and Guidelines on Transboundary Salmon Stocks: Case Study of the Tana River

The year 2019 was “the international year of the salmon” (IYS). The overarching aim was “to inform and stimulate outreach and research that aspires to establish the conditions necessary to ensure the resilience of salmon and people throughout the Northern Hemisphere;” further, to bring people together, share and develop knowledge, raise awareness and take action. This article is intended as a contribution to this goal. The article discusses how international law: the Law of the Sea Convention, the Convention on Biological Diversity and the Convention for the Conservation of Salmon in the North Atlantic Ocean relate to conservation and management of wild salmon. The article has a special focus on bilateral cooperation on salmon stocks in boundary/transboundary rivers, and using as a case study the Tana river in Norway and Finland.

Multi-criteria decision analysis in Bayesian networks - Diagnosing ecosystem service trade-offs in a hydropower regulated river

The paper demonstrates the use of Bayesian networks in multicriteria decision analysis (MCDA) of environmental design alternatives for environmental flows (eflows) and physical habitat remediation measures in the Mandalselva River in Norway. We demonstrate how MCDA using multi-attribute value functions can be implemented in a Bayesian network with decision and utility nodes. An object-oriented Bayesian network is used to integrate impacts computed in quantitative sub-models of hydropower revenues and Atlantic salmon smolt production and qualitative judgement models of mesohabitat fishability and riverscape aesthetics. We show how conditional probability tables are useful for modelling uncertainty in value scaling functions, and variance in criteria weights due to different stakeholder preferences. While the paper demonstrates the technical feasibility of MCDA in a BN, we also discuss the challenges of providing decision-support to a real-world habitat remediation process.

Population genetic analysis reveals a geographically limited transition zone between two genetically distinct Atlantic salmon lineages in Norway.

Atlantic salmon is characterized by a high degree of population genetic structure throughout its native range. However, while populations inhabiting rivers in Norway and Russia make up a significant proportion of salmon in the Atlantic, thus far, genetic studies in this region have only encompassed low to modest numbers of populations. Here, we provide the first “in‐depth” investigation of population genetic structuring in the species in this region. Analysis of 18 microsatellites on >9,000 fish from 115 rivers revealed highly significant population genetic structure throughout, following a hierarchical pattern. The highest and clearest level of division separated populations north and south of the Lofoten region in northern Norway. In this region, only a few populations displayed intermediate genetic profiles, strongly indicating a geographically limited transition zone. This was further supported by a dedicated cline analysis. Population genetic structure was also characterized by a pattern of isolation by distance. A decline in overall genetic diversity was observed from the south to the north, and two of the microsatellites showed a clear decrease in number of alleles across the observed transition zone. Together, these analyses support results from previous studies, that salmon in Norway originate from two main genetic lineages, one from the Barents–White Sea refugium that recolonized northern Norwegian and adjacent Russian rivers, and one from the eastern Atlantic that recolonized the rest of Norway. Furthermore, our results indicate that local conditions in the limited geographic transition zone between the two observed lineages, characterized by open coastline with no obvious barriers to gene flow, are strong enough to maintain the genetic differentiation between them.

CFD modeling as part of a hybrid modeling case study for a gauging station with challenging hydraulics

ABSTRACTEstimates of peak flood discharges are most often based on stage measurements, and the discharge is determined indirectly from the extrapolation of a rating-curve. It is possible to improve the extrapolation of the rating-curve by hydraulic modeling, such as scale or numerical models. In this paper, we discuss a case study for a streamflow gauging station in a mountainous river in Norway. A computational fluid dynamics model is used as part of a hybrid modeling study to study the flow at this gauging station with challenging geometry and flow and derive a reliable rating-curve. The numerical model produces stage-discharge data with comparable accuracy to the scale model. Analysis of the results of the hybrid modeling shows that the original location of stage measurements is not suitable for field measurements or for modeling the rating curve. Based on the modeling, an alternative point of measurement with better flow conditions at high flows is identified. The results and findings highlight the importance of 3-dimensional flow at the site during floods and shows how computational fluid dynamics can be used to provide stage-discharge data for rating curves and provide additional insight in hybrid modeling.

A new Scandinavian Chamaedrilus species (Clitellata: Enchytraeidae), with additional notes on others.

Chamaedrilus (earlier referred to as Cognettia) is a well-known genus of terrestrial and limnic enchytraeids, currently with 19 known species in the world. Some of its species are morphologically cryptic and can only be identified using genetic (DNA) information. Many of them reproduce asexually, and the prevalence of sexual mature individuals is generally low in the populations. Chamaedrilus asloae sp. nov. (Clitellata: Enchytraeidae) is described based on material from two rivers in Norway, one in Sweden, and from a wet deciduous forest in Denmark. With the material at hand, no morphological characters completely separate C. asloae from C. chalupskyi; none of the available specimens of the new species are sexually mature. However, four molecular markers (two mitochondrial, two nuclear) support that C. asloae is a distinct, separately evolved lineage, which is sister to a clade consisting of C. glandulosus and C. varisetosus. In this study, too, the fully developed sexual organs of C. chalupskyi and C. varisetosus are described and illustrated.

Investigating the frequency of triploid Atlantic salmon in wild Norwegian and Russian populations

BackgroundFish may display variations in ploidy, including three sets of chromosomes, known as triploidy. A recent study revealed a frequency of ~ 2% spontaneous (i.e., non-intentional) triploidy in domesticated Atlantic salmon produced in Norwegian aquaculture in the period 2007–2014. In contrast, the frequency of triploidy in wild salmon populations has not been studied thus far, and in wild populations of other organisms, it has been very rarely studied. In population genetic data sets, individuals that potentially display chromosome abnormalities, such as triploids with three alleles, are typically excluded on the premise that they may reflect polluted or otherwise compromised samples. Here, we critically re-investigated the microsatellite genetic profile of ~ 6000 wild Atlantic salmon sampled from 80 rivers in Norway and Russia, to investigate the frequency of triploid individuals in wild salmon populations for the first time.ResultsWe detected a single triploid salmon, and five individuals displaying three alleles at one of the loci, thus regarded as putatively trisomic. This gave an overall frequency of triploid and putatively trisomic individuals in the data set of 0.017 and 0.083% respectively. The triploid salmon was an adult female, and had spent 2 years in freshwater and 2 years in the sea.ConclusionsWe conclude that the frequency of naturally-occurring triploid Atlantic salmon in wild Norwegian and Russian populations is very low, and many-fold lower than the frequency of spontaneous triploids observed in aquaculture. Our results suggest that aquaculture rearing conditions substantially increase the probability of triploidy to develop, and/or permits greater survival of triploid individuals, in comparison to the wild.

Socio-environmental integration of hydropower facilities

Centre for Environmental Design of Renewable Energy (CEDREN) is a research centre focusing on hydropower production and environmental impacts of hydropower. The main objective of CEDREN is to develop and communicate design solutions for renewable energy production that address environmental and societal challenges at local, regional, national and global levels. Environmental design means that planning, building and operation have to include technical, economic, environmental and socio-political aspects throughout the whole life-span of the project. Methods and tools to ensure environmental design are developed in CEDREN and applied to case studies in Norway and internationally. These methods and tools focus on finding physical and biological bottlenecks for affected species and ecosystems by mapping, modelling and analysis of both physical conditions and ecological status. CEDREN proposes different measures, tools and methods to improve the environmental conditions as well as how to maintain or increase the power production. In addition, a strong focus must be made on political governance to ensure more representative participation of relevant stakeholders in the process of finding the best technical, economic and political solutions for power production, the environment and the society. Key research findings used to develop relationships between physical factors like flow, flow fluctuations, water temperature, water velocity, water depth and water-covered area and biological response will be shown. Examples of improved methods for better planning procedures with stakeholder engagement will be proposed. Examples of methods and tools for environmental design of hydropower will be given for several regulated rivers in Norway and abroad.

Duration of the parasitic phase determines subsequent performance in juvenile freshwater pearl mussels (Margaritifera margaritifera).

Host–parasite systems have been useful in understanding coevolutionary patterns in sympatric species. Based on the exceptional interaction of the long‐lived and highly host‐specific freshwater pearl mussel (FPM; Margaritifera margaritifera) with its much shorter‐lived host fish (Salmo trutta or Salmo salar), we tested the hypotheses that a longer duration of the parasitic phase increases fitness‐related performance of mussels in their subsequent post parasitic phase, and that temperature is the main factor governing the duration of the parasitic phase. We collected juvenile mussels from naturally and artificially infested fish from eight rivers in Norway. Excysted juvenile mussels were maintained separately for each collection day, under similar temperature and food regimes, for up to 56 days. We recorded size at excystment, post excystment growth, and survival as indicators of juvenile fitness in relation to the duration of the parasitic phase. We also recorded the daily average temperatures for the entire excystment period. We observed strong positive relationships between the length of the parasitic phase and the post parasitic growth rate, size at excystment and post parasitic survival. Temperature was identified as an important factor governing excystment, with higher temperatures decreasing the duration of the parasitic phase. Our results indicate that juvenile mussels with the longest parasitic phase have better resources (larger size and better growth rate) to start their benthic developmental phase and therefore to survive their first winter. Consequently, the parasitic phase is crucial in determining subsequent survival. The temperature dependence of this interaction suggests that climate change may affect the sensitive relationship between endangered FPMs and their fish hosts.

Passing a seawater challenge test is not indicative of hatchery-reared Atlantic salmon Salmo salar smolts performing as well at sea as their naturally produced conspecifics.

Despite satisfactory reactions to seawater challenge tests indicative of appropriate physiological state, hatchery-reared Atlantic salmon Salmo salar smolts stocked in the Eira River in Norway between 2001 and 2011 performed less well at sea in terms of growth, age at maturity and survival than smolts of natural origin. The mean rates of return to the river for hatchery-reared and naturally produced S. salar were 0·98 and 2·35%. In the Eira River, c. 50 000 hatchery-reared S. salar smolts of local origin were stocked annually to compensate for reduced natural smolt production following regulation for hydroelectric purposes, while a mean of 17 262 smolts were produced naturally in the river. This study demonstrates that, although captive S. salar perform well in seawater challenge tests, hatchery-reared smolts are not necessarily as adaptable to marine life as their naturally produced counterparts. These findings suggest that production of hatchery-reared smolts more similar to naturally produced individuals in morphology, physiology and behaviour will be necessary to improve success of hatchery releases. Where possible, supplementary or alternative measures, including habitat restoration, could be implemented to ensure the long-term viability of wild stocks.

Somatic hypermutation introduces a high frequency of amino acid substitutions in the constant region of the T cell receptor alpha chain in ballan wrasse (Labrus bergylta)

It was previously thought that T cell receptor genes were not subject to somatic hypermutation, which is in contrast to immunoglobulin genes where this mechanism is associated with a key role in the affinity maturation of the antibody response. However, recent studies in shark and camels have shown that variable T cell receptor gamma chain genes are hypermutated. In this presentation we show that somatic mutations on the constant part of the T cell receptor alpha chain gene are frequent in a teleost fish; Ballan wrasse (Labrus bergylta). We examined many Tcra cDNAs from the same individual to determine if constant region diversity exceeded the amount that could be attributed to germline polymorphism. It is plausible to assume that somatic hypermutation is involved in the generation of the T cell repertoire and that consequently mutations are introduced in the constant region gene. The amino acid substitutions we found in Ballan wrasse are primarily positioned in the loops between the beta sheets, i.e. regions that apparently are not critical for the overall structure. It has been reported that polymorphism at the constant T cell receptor alpha chain genes is widespread in teleosts. After we had found several amino acid substitutions in translated cDNAs present in public databases the present study was initiated in an attempt to reveal corresponding polymorphism in Atlantic salmon (Salmo salar). Although we sequenced the gene in several individuals from rivers in Norway, Russia and Canada we only found some germline mutations in untranslated regions.