This study reports the physico-chemical characteristics in Cauvery River, Noyyal River, and Chennai Lakes in Tamil Nadu, India. Surface water samples were collected and analyzed as per standard methods. The parameters analyzed were pH, temperature, turbidity, electrical conductivity, chemical oxygen demand, biological oxygen demand, dissolved oxygen, total solids, total dissolved solids, total suspended solids, chloride, sodium, potassium, total alkalinity, total hardness, calcium, magnesium, fluoride, sulphate, and phosphate. The results of the chemical analysis indicate that the study area contains chloride, calcium, magnesium, sulphate, sodium, potassium, bicarbonate, and phosphate ions. The concentration of fluoride ion is quite alarming in Noyyal River and Pallavaram Lake. In addition, most probable number for coliforms was analyzed and the results were found beyond the permissible limit. The suitability of surface water for irrigation was studied by Wilcox, USSL and Gibbs plot. From this plot we found that the study area of Noyyal River is unfit for irrigation. The overall study indicated that the River Noyyal was largely polluted by anthropogenic input due to industrial effluents, municipal sewage, and other human activities.