Risk Of Disease Transmission Research Articles

SEIR Model Based Epidemic Transmission Risk Deep Prediction

Amidst ongoing efforts to manage the spread of infectious diseases, measures are taken to safeguard the resumption of on-campus activities. This ensures the academic continuity and the safety of the community. To achieve this, an analysis of disease transmission risks has been conducted, focusing on areas within campuses such as dining halls, lecture theaters, and classrooms. By leveraging an enhanced Susceptible-Exposed-Infectious-Removed (SEIR) model, we have developed a risk assessment model that takes into account dynamics between susceptible, latent, infected and displaced groups. After the extraction of relevant features, features undergo preprocessing steps. They are monotonically incremented and smoothed to eliminate noise, and then serve as input and labels for training stacked denoising autoencoder. The outcome of analysis indicates that the implementation of interventions can significantly mitigate the spread of disease. It can decrease the frequency of infection interactions, lower the transmission rate, and reduce the peak numbers of infected and latent cases by 61% and 72%, respectively. In essence, our approach has proven to be effective in controlling the spread of diseases in key university areas. Moreover, it provides an accurate predictive model for the number of infections, offering a valuable tool for managing and preventing outbreaks within these communities.

Spatial and Temporal Dynamics of the Canine Population and Contamination by Canine Feces on an Urban Beach

Objective: The objective of this study was to analyze the spatio-temporal distribution of dogs and feces using the visual census method. The research aimed to quantify the presence of dogs and the number of feces observed in these environments. Method: The visual census was conducted every fifteen days over the course of one year along the Mar Grosso beach shoreline, which was divided into areas and sectors. The data were subsequently tabulated, and statistical analysis was performed using the non-parametric Kruskal-Wallis test. Research Results and Discussions: The highest number of feces and stray dogs were found in the urban sector during the summer. Regarding the areas observed and feces, the area with the highest number of feces was A3 in the urban sector during the summer. As for the number of stray, domiciled, and off-leash dogs, they were more frequently observed in the summer, with stray dogs predominantly in A2 (beach sector), and domiciled and off-leash dogs in A3 and A2, respectively, in the urban sector. Research Implications: The results identify hotspots of feces and dogs along the study area, providing valuable information that can assist in decision-making and the implementation of public policies aimed at controlling animals on beaches and protecting the environment, thereby reducing the risk of disease transmission to the population frequenting these locations. Originality/Value: This study contributes to evidence-based policy implementation to promote environmental health, public health, and animal welfare in coastal areas.

Do reusables pose greater infectious risks than disposables for consumer goods? A systematic literature review

Increased concern over climate change and the emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus resulted in a clash of political directives around reusable and disposable food serviceware. Decreasing single-use items will likely reduce consumption and environmental emissions; however, improper cleaning of reusable items could result in greater risks of disease transmission. We sought to assess the risks of reusable and disposable food serviceware and document disinfection protocols by conducting a systematic literature review of articles that assessed materials or products that could be fomites to specific food-borne pathogens. After initial screening, the study team extracted data from 122 articles. The most common pathogens studied were E. coli (25% of included studies), general bacteria (24%), and Norovirus (16%). Thirteen studies (8%) focused on SARS-CoV-2. A majority of studies analyzed plastics (27%), stainless steel (22%), or porous surfaces such as paper and cardboard (12%). Forty seven of the studies (35%) were conducted in a food service setting, and 40 studies (30%) tested disinfection techniques. Despite a large body of related literature, there is very little evidence suggesting that either reusable or disposable food serviceware is safer for minimizing infectious risks. Pathogens can survive on various fomites, though greater surface porosity and higher humidity levels increase viability of most pathogens. There appear to be no major differences in pathogen viability on various fomites. There is a paucity of research that can specifically aid in developing policy or guidelines for appropriate use of reusable food serviceware. Though given recent studies on SARS-CoV-2, banning reusable bags and food serviceware is an inappropriate response to this particular pathogen, which is rarely spread through surface contact. Further research is needed that explicitly studies pathogen viability, transmission risks, and appropriate disinfection techniques for disposable and reusable food serviceware in order to devise effective sustainability policies.

Comparison of utility and organizational impact of reusable and single-use rhinolaryngoscopes in a tertiary otorhinolaryngology department.

Flexible rhinolaryngoscopes are an important tool in otolaryngology. In recent years, single-use rhinolaryngoscopes (SURLs), which have been developed as an alternative to reusable scopes (RRLs), offer various advantages including less risk of contamination and elimination of the need for cleaning and reprocessing between procedures. This study aimed to compare procedure efficiency, organizational impact, and economic impact between SURLs and RRLs used for elective procedures conducted outside the otorhinolaryngology department in the hospital environment. In this randomized prospective study, either type of endoscope was tested by on-call otolaryngologists over a total of twelve weeks. The organizational impact was investigated using a quantitative research design. All categories of stakeholders responded to specific surveys based on profession; these included doctors (n = 13), those in managerial positions (n = 3), and other healthcare staff including technicians and nurses (n = 11). A micro-costing approach was used to evaluate resource utilization and cost of services. The trial was uploaded to clinicaltrials.gov (ID number: NCT0519821, https://clinicaltrials.gov/study/NCT05198219?intr=rhinolaryngo&rank=1). Overall, 14 and 12 procedures were performed using the SURLs and RRLs, respectively. No significant differences were observed between the endoscopes in terms of procedure duration, reported image quality, or maneuverability. The SURLs were significantly superior in terms of four organizational impact parameters, namely, modes of cooperation and communication, vigilance and monitoring methods, working conditions and safety, and logistics. The estimated per-procedure cost of the RRLs was SEK 536 (€ 34,68). The cost and effectiveness of RRLs and SURLs is influenced by the healthcare setting, procedure volume, and duration of device use. The adoption of SURLs can improve safety, streamline processes, and potentially reduce the risk of disease transmission.

Assessing measles risk transmission in Iran: a utilization of the World Health Organization’s programmatic risk assessment tool,2022

BackgroundDespite successful efforts to eliminate measles in Iran, imported measles cases continue to be reported. Because measles is endemic in neighboring countries. This research aims to evaluate the risk of measles transmission in different regions of Iran.MethodsMeasles case-based surveillance data of the Expanded Program of Immunization containing 31 provinces and 463 districts from 2019 to 2021 were assessed. The WHO Measles Programmatic Risk Assessment tool was used to evaluate the risk of disease transmission in four domains: population immunity, surveillance quality, program delivery performance, and threat assessment. scores were categorized as low, medium, high, or very high risk.ResultsDuring 2019–2021, the average incidence of measles was 1.9 per 1 million. Chabahar and Mashhad with 76 and ./6per million reported the highest and lowest incidence respectively. All 463 districts were categorized as low risk in risk assessment. Andimeshk, Chabahar, and Bojnurd obtained the highest risk scores with 27, 24, and 25 respectively. All districts were classified as low risk for population immunity. The average coverage of (MMR1) and (MMR2) was 95% or higher. All districts received the minimum points for surveillance quality.ConclusionAll regions are placed at a low level of disease transmission risk. However, the tool is not able to assess the risk at the rural or peripheral sectors level. The indicators used in this tool are the same for all countries with different epidemiological features (elimination, endemic). Sensitivity analysis can optimize the use of this tool for countries with different disease conditions.

Spatial distribution of poultry farms using point pattern modelling: A method to address livestock environmental impacts and disease transmission risks.

The distribution of farm locations and sizes is paramount to characterize patterns of disease spread. With some regions undergoing rapid intensification of livestock production, resulting in increased clustering of farms in peri-urban areas, measuring changes in the spatial distribution of farms is crucial to design effective interventions. However, those data are not available in many countries, their generation being resource-intensive. Here, we develop a farm distribution model (FDM), which allows the prediction of locations and sizes of poultry farms in countries with scarce data. The model combines (i) a Log-Gaussian Cox process model to simulate the farm distribution as a spatial Poisson point process, and (ii) a random forest model to simulate farm sizes (i.e. the number of animals per farm). Spatial predictors were used to calibrate the FDM on intensive broiler and layer farm distributions in Bangladesh, Gujarat (Indian state) and Thailand. The FDM yielded realistic farm distributions in terms of spatial clustering, farm locations and sizes, while providing insights on the factors influencing these distributions. Finally, we illustrate the relevance of modelling realistic farm distributions in the context of epidemic spread by simulating pathogen transmission on an array of spatial distributions of farms. We found that farm distributions generated from the FDM yielded spreading patterns consistent with simulations using observed data, while random point patterns underestimated the probability of large outbreaks. Indeed, spatial clustering increases vulnerability to epidemics, highlighting the need to account for it in epidemiological modelling studies. As the FDM maintains a realistic distribution of farm location and sizes, its use to inform mathematical models of disease transmission is particularly relevant for regions where these data are not available.

Development of a P(L-D,L)LA Foam as a Dura Substitute and Its In Vitro Evaluation

Dura substitutes are used to reduce the risk of postoperative complications following neurosurgical interventions, and to facilitate the healing of dura damages or defects caused by injuries. Traditional tissue transplants have limitations like limited tissue availability, potential risk of immune rejection and disease transmission. The use of biomaterials composed of synthetic polymers as dura substitutes offers a promising approach to overcome these limitations to replace and treat damaged dura mater. Potential biocompatible porous scaffolds still need to be developed to minimize the risks of immune response and disease transmission, while also ensuring effective cell migration and cell ingrowth in three dimension. The aim of the present study was to develop a poly(L-lactide-co-D,L-lactide) (P(L-D,L)LA) foam with an optimal pore size for dura mater substitution, investigate its morphological characteristics, and evaluate its potential for dura mater regeneration by assessing the spreading and growth of meningeal cells within it through in vitro studies. Foams were produced by lyophilization using different concentrations of P(L-D,L)LA solution. A GMP-grade P(L-D,L)LA, suitable for medical device applications, was used in this study. Morphological analysis was performed using scanning electron microscopy, and porosity of the foams was studied with mercury porosimetry. In in vitro studies, meningeal cells were seeded onto the polymeric foams, and their behavior and proliferation in these scaffolds were investigated with cytoskeleton and nucleus staining, and colorimetric cell proliferation assay, respectively. Scanning electron microscopy results showed that the foams prepared with 2.5% and 3% polymer solutions displayed good structural integrity and convenient interconnectivity, with pore sizes ranging from 80 to 150 µm. However, the foams prepared with 2% and 4% polymer solution demonstrated poor structural integrity and low interconnectivity, respectively. In vitro studies showed that the foams prepared with 2.5% and 3% polymer solutions served effectively as scaffolds for meningeal cells, and the cells attached, spread and homogeneously distributed. In addition, the cells proliferated and increased in number over time within these polymeric scaffolds. These findings suggest that the foams produced with 2.5% and especially 3% P(L-D,L)LA polymer solutions could effectively serve as a suitable substitute for the dura mater, providing an appropriate environment for cell ingrowth and tissue integration. This indicates that the developed foam could be a promising treatment for dura mater damage or defects, with the potential approach to promote regeneration in future in vivo and clinical studies.

Therapeutic Effect of Decellularized Extracellular Matrix from Fish Skin for Accelerating Skin Regeneration.

A cellular matrix derived from natural tissue functions as a highly biocompatible and versatile material for wound healing application. It provides a complex and highly organized environment with biological molecules and physical stimuli. Recently, various kinds of tissue/organ decellularized extracellular matrixes (dECMs) from bovine and porcine have been used as biomedical applications to support tissue regeneration but inherit religious restrictions and the risk of disease transmission to humans. Marine fish-derived dECMs are seen as attractive alternatives due to their similarity to mammalian physiology, reduced biological risks, and fewer religious restrictions. The aim of this study was to derive a decellularized matrix from the olive flounder (Paralichthys olivaceus) skin and evaluate its suitability as a wound healing application. Olive flounder skin was treated with a series of chemical treatments to remove cellular components. Decellularized fish skin (dFS) was confirmed to be successful in decellularization by evaluating the DNA content (2.84%). The dFS was characterized and evaluated in vivo to assess its biological activities. The mouse wound defect model was used to evaluate the in vivo performance of the dFS compared with that of the decellularized porcine skin (dPS). The resultant dFS was shown to enhance wound healing compared with the no-treatment group and dPS. This study suggests that dFS has potential for skin regeneration application.

Relating Wildlife Camera Trap Data to Tick Abundance: Testing the Relationship in Different Habitats.

The increase in acarological risk of tick bites is significantly driven by profound changes in landscape, which alter the density and distribution of wildlife that support tick populations. As a result of habitat shifts and land abandonment, which create environments conducive to tick proliferation, the risk of disease transmission to humans and animals is increasing. In this context, it is important to explore tick ecology by applying a comprehensive methodology. In this study, we examined the relationship between wildlife temporal occupancy and tick abundance in two distinct regions: an alpine hunting district and a natural park in the Apennines. For each sampling point, we calculated wildlife temporal occupancy from camera trap pictures and estimated ticks' abundance from dragging transects in the area immediately surrounding camera traps. In modelling the relationship between those two variables, we included abiotic factors such as saturation deficit, normalized difference vegetation index, and altitude. Results show the importance of altitude and wildlife temporal occupancy (itself related to different habitat and land management characteristics) on the ecology of questing ticks. If employed in management decisions for natural environments, such information is useful to modulate the acarological risk and thus the risk of tick-borne pathogens' transmission.

Exploring the influence of environmental indicators and forecasting influenza incidence using ARIMAX models.

Influenza is a respiratory infection that poses a significant health burden worldwide. Environmental indicators, such as air pollutants and meteorological factors, play a role in the onset and propagation of influenza. Accurate predictions of influenza incidence and understanding the factors influencing it are crucial for public health interventions. Our study aims to investigate the impact of various environmental indicators on influenza incidence and apply the ARIMAX model to integrate these exogenous variables to enhance the accuracy of influenza incidence predictions. Descriptive statistics and time series analysis were employed to illustrate changes in influenza incidence, air pollutants, and meteorological indicators. Cross correlation function (CCF) was used to evaluate the correlation between environmental indicators and the influenza incidence. We used ARIMA and ARIMAX models to perform predictive analysis of influenza incidence. From January 2014 to September 2023, a total of 21,573 cases of influenza were reported in Fuzhou, with a noticeable year-by-year increase in incidence. The peak of influenza typically occurred around January each year. The results of CCF analysis showed that all 10 environmental indicators had a significant impact on the incidence of influenza. The ARIMAX(0, 0, 1) (1, 0, 0)12 with PM10(lag5) model exhibited the best prediction performance, as indicated by the lowest AIC, AICc, and BIC values, which were 529.740, 530.360, and 542.910, respectively. The model achieved a fitting RMSE of 2.999 and a predicting RMSE of 12.033. This study provides insights into the impact of environmental indicators on influenza incidence in Fuzhou. The ARIMAX(0, 0, 1) (1, 0, 0)12 with PM10(lag5) model could provide a scientific basis for formulating influenza control policies and public health interventions. Timely prediction of influenza incidence is essential for effective epidemic control strategies and minimizing disease transmission risks.

Rewiring cattle movements to limit infection spread

Cattle tracing databases have become major resources for representing demographic processes of livestock and assessing potential risk of infections spreading by trade. The herds registered in these databases are nodes of a network of commercial movements, which can be altered to lower the risk of disease transmission. In this study, we develop an algorithm aimed at reducing the number of infected animals and herds, by rewiring specific movements responsible for trade flows from high- to low-prevalence herds. The algorithm is coupled with a generic computational model based on the French cattle movement tracing database (BDNI), and used to describe different scenarios for the spread of infection within and between herds from a recent outbreak (epidemic) or a five-year-old outbreak (endemic). Results show that rewiring successfully contains infections to a limited number of herds, especially if the outbreak is recent and the estimation of disease prevalence frequent, while the respective impact of the parameters of the algorithm depend on the infection parameters. Allowing any animal movement from high to low-prevalence herds reduces the effectiveness of the algorithm in epidemic settings, while frequent and fine-grained prevalence assessments improve the impact of the algorithm in endemic settings. Our approach focusing on a few commercial movements is expected to lead to substantial improvements in the control of a targeted disease, although changes in the network structure should be monitored for potential vulnerabilities to other diseases. This general algorithm could be applied to any network of controlled individual movements liable to spread disease.

Use of autologous dentin matrix in bone defects augmentation - a literature review.

A number of regenerative materials are currently used to regenerate or preserve the alveolar pro- cess. One of these is autogenous dentin matrix. With many valuable properties such as easy availability, simple preparation, low cost, low risk of disease transmission and no risk of triggering an immune response against the graft, autogenous dentin matrix appears to be a very good material of choice. The following article is intended to provide an overview of the use of autogenous dentin matrix.

Effective methods for the decontamination of healthcare waste: Ozone and UV-C radiation process

ABSTRACT Human-generated waste, including infectious healthcare waste, poses significant risks to public health and the environment. The COVID-19 pandemic has increased the global production of infectious waste, emphasizing the need for safe and sustainable waste management practices. While autoclaves are commonly used for on-site disposal, alternative methods like ozone gas and UV-C radiation offer environmentally friendly options that effectively eliminate pathogens without leaving toxic residues. Inadequate waste management can contribute to disease transmission, while open burning releases harmful pollutants. This study investigated the effectiveness of different disinfection agents – ozone gas and UV-C radiation – on infectious solid waste contaminated with bacteria. The bacterial indicators examined were Staphylococcus aureus, Bacillus subtilis, and Pseudomonas aeruginosa. The experimental methods included operating each ozone and UV-C radiation individually and simultaneously using ozone gas and UV-C radiation. The study also investigated exposure times and various concentrations of ozone gas. The findings demonstrated that the simultaneous application of ozone gas and UV-C radiation was the most effective method for decontaminating infectious solid waste and targeting the selected bacteria. The concentration of ozone gas ranged from 165 to 5000 ppm, depending on generation time and treatment chamber volume, while exposure times varied from 1 to 180 minutes. In applying UV-C rays, complete elimination of S. aureus was observed after 60 minutes up to 6-log, while the reduction of B. subtilis and P. aeruginosa were 2-log and 3-log, respectively. Ozone gas had the ability to inactivate all strains, but when ozone gas and UV-C rays were used simultaneously, this process was accelerated and improved. The total reduction in the bacterial load was 8-log. Considering the increase in population and the subsequent increase in waste generation, adopting an environmentally friendly waste management method can be very advantageous. Implications: This study highlights the effectiveness of simultaneously applying ozone gas and UV-C radiation for decontaminating infectious solid waste, offering an environmentally friendly alternative to traditional thermal treatments like autoclave and incineration. By optimizing ozone concentrations and exposure times, this method reduces disease transmission risks and minimizes environmental impact. These findings are crucial, especially during outbreaks such as the COVID-19 pandemic, providing scalable, sustainable waste management solutions for healthcare facilities. Implementing these techniques can protect public health and the environment, setting a new standard for safe infectious waste disposal worldwide, mitigating hazardous pollutants, and reduce the exposure risk of bio-hazardous residues.

Pregnancy in women with mitochondrial disease-A literature review and suggested guidance for preconception and pregnancy care.

Mitochondrial donation to reduce the risk of primary mitochondrial disease transmission from mother to child is now permitted under Australian law as part of a clinical trial. The energy demands of pregnancy have the potential to worsen mitochondrial disease symptoms and severity in affected women. We conducted a systematic literature review on mitochondrial disease in pregnancy; five cohort studies and 19 case reports were included. For many women with mitochondrial disease, pregnancy does not have a negative effect on health status. However, serious adverse outcomes may occur. We provide suggested guidelines for preconception counselling and antenatal care.

Mosquito dynamics and their drivers in peri-urban Antananarivo, Madagascar: insights from a longitudinal multi-host single-site survey

BackgroundAntananarivo, the capital city of Madagascar, is experiencing a steady increase in population growth. Due to the abundance of mosquito vectors in this locality, the population exposed to mosquito-borne diseases is therefore also increasing, as is the risk of epidemic episodes. The aim of the present study was to assess, in a resource-limited setting, the information on mosquito population dynamics and disease transmission risk that can be provided through a longitudinal entomological study carried out in a multi-host single site.MethodsMosquitoes were collected every 15 days over 16 months (from January 2017 to April 2018) using six CDC-light traps in a peri-urban area of Antananarivo. Multivariable generalised linear models were developed using indoor and outdoor densities of the predominant mosquito species as response variables and moon illumination, environmental data and climatic data as the explanatory variables.ResultsOverall, 46,737 mosquitoes belonging to at least 20 species were collected, of which Culex antennatus (68.9%), Culex quinquefasciatus (19.8%), Culex poicilipes (3.7%) and Anopheles gambiae sensu lato (2.3%) were the most abundant species. Mosquito densities were observed to be driven by moon illumination and climatic factors interacting at different lag periods. The outdoor models demonstrated biweekly and seasonal patterns of mosquito densities, while the indoor models demonstrated only a seasonal pattern.ConclusionsAn important diversity of mosquitoes exists in the peri-urban area of Antananarivo. Some well-known vector species, such as Cx. antennatus, a major vector of West Nile virus (WNV) and Rift-Valley fever virus (RVFV), Cx. quinquefasciatus, a major vector of WNV, Cx. poicilipes, a candidate vector of RVFV and An. gambiae sensu lato, a major vector of Plasmodium spp., are abundant. Importantly, these four mosquito species are present all year round, even though their abundance declines during the cold dry season, with the exception of Cx. quinquefasciatus. The main drivers of their abundance were found to be temperature, relative humidity and precipitation, as well as—for outdoor abundance only—moon illumination. Identifying these drivers is a first step towards the development of pathogen transmission models (R0 models), which are key to inform public health stakeholders on the periods of most risk for vector-borne diseases.Graphical

Bolstering community resilience through health-focused climate change adaptation: moving from talk to action in Western Canadian communities

ABSTRACT The impacts of climate change have been recognized as a global health emergency. Worsening climate stressors are resulting in injury, illness and death. As this threat to health grows, so does the need to adapt. Climate change adaptation has been noted to reduce the risk of disease transmission, chronic illness exacerbation, physical trauma, and the mental health impacts associated with climate change. Cities across Western Canada have initiated the process of implementing health-focused climate change adaptation; however, progress has been slow, leaving communities vulnerable to health threats. Exploring five case study communities in Western Canada, this research sheds light on factors that enable and constrain progress on health-focused climate change adaptation. Research objectives are addressed through analysis of 16 key actor interviews (with experts in public health, planning, local governance, and other related fields), and a scan of relevant strategic planning documents. Results indicate that political will, expertise, and awareness has resulted in the development of health-focused climate change adaptation policy/ plans. However, implementation of these plans lags in practice. This lag stems from a lack of implementation detail in climate change adaptation plans, limited understanding of the impact of climate change on health, and jurisdictional confusion.

Bayesian spatio-temporal modeling to assess the effect of land-use changes on the incidence of Cutaneous Leishmaniasis in the Brazilian Amazon

Cutaneous Leishmaniasis (CL) is a vector-borne disease caused by a protozoan of the genus Leishmania and is considered one of the most important neglected tropical diseases. The Brazilian Amazon Forest harbors one of the highest diversity of Leishmania parasites and vectors and is one of the main focuses of the disease in the Americas. Previous studies showed that some types of anthropogenic disturbances have affected the abundance and distribution of CL vectors and hosts; however, few studies have thoroughly investigated the influence of different classes of land cover and land-use changes on the disease transmission risk. Here, we quantify the effect of land use and land-cover changes on the incidence of CL in all municipalities within the Brazilian Amazon Forest, from 2001 to 2017. We used a structured spatiotemporal Bayesian model to assess the effect of forest cover, agriculture, livestock, extractivism, and- deforestation on CL incidence, accounting for confounding variables such as population, climate, socioeconomic, and spatiotemporal random effects. We found that the increased risk of CL was associated with deforestation, especially modulated by a positive interaction between forest cover and livestock. Landscapes with ongoing deforestation for extensive cattle ranching are typically found in municipalities within the Amazon Frontier, where a high relative risk for CL was also identified. These findings provide valuable insights into developing effective public health policies and land-use planning to ensure healthier landscapes for people.

Beliefs, taboos, usages, health perceptions, and practices toward wildlife among different ethnicities in Tak and Mae Hong Son Provinces, Thailand

This concurrent qualitative study was carried out with the main objective to determine wildlife beliefs, taboos, usages, health perceptions, and practices among ten ethnic groups in four communities in Tak and Mae Hong Son provinces of Thailand from November 2020 to January 2021. We also gathered comprehensive information on study respondents’ knowledge related to potential risk behaviors that could lead to zoonotic disease transmission and infection. Furthermore, we intended to use the study’s findings to develop communication strategies and health literacy improvement interventions for mitigating risky behaviors, with a focus on ethnic groups and particular individuals who live in close proximity to forests and wildlife, to prevent future pandemics. Sixty-five respondents were purposively selected based on their extensive knowledge, active participation in local cultural contexts, beliefs, and exposure to wildlife contact or consuming game animals. Twenty (30.8%) participated in in-depth interviews (IDIs), while 45 (69.2%) participated in eight focused group discussions (FGDs). The results revealed that the characteristics of wildlife contact are similar and distinct based on their beliefs and taboos among various ethnic groups and study locations, which are influenced by cultural backgrounds and traditions. Although some ethnic groups do not have explicit restrictions on the consumption of wildlife, others adhere to specific beliefs and taboos that forbid the consumption or killing of specific wild animals. These beliefs frequently correspond with conservation initiatives, thereby facilitating the preservation of threatened species. The study also revealed a lack of appropriate health knowledge, perceptions, and practices regarding wildlife contact and consumption. As a result, it is recommended that public health officials and local governments develop and execute communication and education initiatives. These campaigns should aim to increase health literacy and promote safe handling, preparation, and cooking practices to reduce the risk of zoonotic disease transmission and infection effectively. Moreover, it is necessary to design and implement wildlife conservation education and outreach activities. The programs should promote environmental stewardship while considering the cultural contexts, beliefs, and practices of various ethnic groups. The activities should involve diverse stakeholders, including local leaders, religious influencers, community members, schoolteachers, students, health professionals, village health volunteers, and civil society organizations.

Risk of BSE transmission when fishmeal derived from fish fed bovine spray-dried red blood cells is included in calf milk replacers

The use of residual streams from agricultural production and food consumption containing animal proteins entails the risk of disease transmission as illustrated by the epidemics of bovine spongiform encephalopathy (BSE) and African swine fever. To combat this risk, the use of animal proteins in livestock feed was banned in the European Union, resulting in a drain of valuable proteins from the agricultural system. With an increasing call for a circular food system, the use of residual streams as a feed ingredient needs to be reconsidered with the associated disease risks being assessed and mitigated where needed. In this study, we assessed the BSE risk of bovine spray-dried red blood cells (SDRBC) as an ingredient of aquafeed. Fish fed with bovine SDRBC could indirectly result in exposure of ruminants to BSE infectivity because one of the exemptions of the feed ban is the use of fishmeal as an ingredient in calf milk replacers. A quantitative risk model was built to evaluate the BSE infectivity present in blood sourced from a slaughtered BSE-infected cow and the reduction of infectivity due to processing steps along the production chain. The end point of the model was the BSE infectivity, expressed in cattle oral ID50 (CoID50), reaching calves fed calf milk replacer containing fishmeal, and the corresponding probability that this will result in at least one new BSE infection.The expected BSE infectivity in blood from a BSE-infected cow at the clinical end state of infection is 0.75 CoID50 (median value). Infectivity in blood mainly results from cross-contamination with brain tissue during stunning at the slaughterhouse. The initial infectivity is reduced along the pathway from slaughtered cow to calf milk replacer, with the highest reduction achieved by clearance of infectivity by fish fed bovine SDRBC as an ingredient of aquafeed, although this parameter has high uncertainty. The final infectivity reaching calves via inclusion of fishmeal in calf milk replacer is estimated to be very low (median value: 1.1 × 10−5 CoID50). Assuming an exponential dose-response model, this corresponds with an expected probability that < 10 out of a million slaughtered BSE-infected cows will result in new BSE infections, which is far below the threshold value of 1 for the basic reproduction number (R0) to initiate a new epidemic. We thus conclude that it is very unlikely that the use of bovine SDRBC as ingredient of aquafeed will result in a new BSE epidemic in cattle. What-if analysis indicated that this conclusion is robust, despite high uncertainty for some input parameters.

Enhancing bone regeneration: Unleashing the potential of magnetic nanoparticles in a microtissue model.

Bone tissue engineering addresses the limitations of autologous resources and the risk of allograft disease transmission in bone diseases. In this regard, engineered three-dimensional (3D) models emerge as biomimetic alternatives to natural tissues, replicating intracellular communication. Moreover, the unique properties of super-paramagnetic iron oxide nanoparticles (SPIONs) were shown to promote bone regeneration via enhanced osteogenesis and angiogenesis in bone models. This study aimed to investigate the effects of SPION on both osteogenesis and angiogenesis and characterized a co-culture of Human umbilical vein endothelial cells (HUVEC) and MG-63 cells as a model of bone microtissue. HUVECs: MG-63s with a ratio of 4:1 demonstrated the best results among other cell ratios, and 50 μg/mL of SPION was the optimum concentration for maximum survival, cell migration and mineralization. In addition, the data from gene expression illustrated that the expression of osteogenesis-related genes, including osteopontin, osteocalcin, alkaline phosphatase, and collagen-I, as well as the expression of the angiogenesis-related marker, CD-31, and the tube formation, is significantly elevated when the 50 μg/mL concentration of SPION is applied to the microtissue samples. SPION application in a designed 3D bone microtissue model involving a co-culture of osteoblast and endothelial cells resulted in increased expression of specific markers related to angiogenesis and osteogenesis. This includes the design of a novel biomimetic model to boost blood compatibility and biocompatibility of primary materials while promoting osteogenic activity in microtissue bone models. Moreover, this can improve interaction with surrounding tissues and broaden the knowledge to promote superior-performance implants, preventing device failure.