This paper present the fresh and harden property of human hair fiber reinforce concrete. From the ancient time many researcher’s and investors carried out research to enhance the physical and mechanical property of concrete. Now a day’s human hair is considered as a waste material and an alternative non degradable matter available in abundance at cheap coast. Present study have been under taken to study the effect of human hair on medium grade concrete on basis of comprehensive strength, effect of crack control and reduce environmental problem. Further Rise Husk Ash (RHA) is a Pozzolonic material used as cement replacement in concrete. Experiments were conducted on concrete on standard cube size with addition of various 0, 0.75, 1.5 and 2.25 % of human hair fiber i.e. by weight of cement and RHA with 0, 5,10 15 and 20% partial replacement of cement. This investigation demonstrated significant improvement in the properties of concrete by the addition of human hair as fiber reinforcement concrete.