The Engineering Sciences for Biology and Medicine (ESBM) is the world’s largest international society of biomedical engineers, which provides a platform for researchers all over the world to share ideas and knowledge regarding the development and applications of engineering concepts and methods toward the solution of biological, medical, and healthcare problems. In 2018, the <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">IEEE EMBS R9 Monterrey Chapter</i> co-sponsored the first edition of the International Congress on NanoBioEngineering (ICNBE’18), organized by the Universidad Autonoma de Nuevo Leon through the School of Chemical Sciences, as the inaugural event of their new Research Center on Biotechnology and Nanotechnology (CIByN). This event was held at the Research Center on Biotechnology and Nanotechnology (CIByN), in Monterrey, Mexico, from the November 7–9, 2018. The ICNBE’18 served as an initial platform to foster interactions between scientists, engineers, and medical researchers and to discuss the advances and future applications of NanoBioEngineering Research. At the ICNBE’18, there were 10 plenary conferences imparted by globally recognized scientists in the fields of nanotechnology, biotechnology, and engineering; there was also a discussion forum on the relevance of interdisciplinary research between these three areas of knowledge and their contribution to solving the most important global problems. In addition, there were more than 70 scientific conferences in oral mode, three specialized workshops, and more than 50 scientific posters were presented; all of these with the aim to encourage discussions and exchange of ideas on the following areas: NanoBioMaterials, Nanotechnology Systems, Nanotechnology and Nanoscience, NanoBioChemistry, Environment and Sustainable Energy, NanoBioMedicine, and NanoBioEngineering. The editors thank all the participants for their appreciated contributions. This special issue selected peer-reviewed articles submitted from the ICNBE’18, and a total of six articles were selected for publication at the IEEE Transactions on Nanobioscience after the rigorous IEEE independent peer review process.