Rigid Groups Research Articles

Integration of a bulky adamantane unit with 9-phenyl-9H-3,9′-bicarbazole: a novel host design for solution-processed narrowband TADF-OLEDs

A p-type host, CzCzPh-mAd, incorporating a bulky and rigid adamantane group, demonstrates promising performance stability for high-efficiency solution-processed OLEDs.

A flexible ligand and halogen engineering enable one phosphor-based full-color persistent luminescence in hybrid perovskitoids.

Color-tunable room temperature phosphorescent (RTP) materials have raised wide interest due to their potential application in the fields of encryption and anti-counterfeiting. Herein, a series of CdX2-organic hybrid perovskitoids, (H-apim)CdX3 and (apim)CdX2 (denoted as CdX-apim1 and CdX-apim2, apim = 1-(3-aminopropyl)imidazole, X = Cl, Br), were synthesized using apim with both rigid and flexible groups as ligands, which exhibit naked-eye detectable RTP with different durations and colors (from cyan to red) by virtue of different halogen atoms, coordination modes and the coplanar configuration of flexible groups. Interestingly, CdCl-apim1 and CdX-apim2 both exhibit excitation wavelength-dependent RTP properties, which can be attributed to the multiple excitation of imidazole/apim, the diverse interactions with halogen atoms, and aggregated state of imidazoles. Structural analysis and theoretical calculations confirm that the aminopropyl groups in CdCl-apim1 do not participate in luminescence, while those in CdCl-apim2 are involved in luminescence including both metal/halogen to ligand charge transfer and twisted intramolecular charge transfer. Furthermore, we demonstrate that these perovskitoids can be applied in multi-step anti-counterfeiting, information encryption and smart ink fields. This work not only develops a new type of perovskitoid with full-color persistent luminescence, but also provides new insight into the effect of flexible ligands and halogen engineering on the wide-range modulation of RTP properties.

Comparison of skeletal stability with rigid and wire fixation in patients who have undergone Le Fort I and Anterior Segmental Maxillary Osteotomy

ABSTRACT Background: Maxillary superior repositioning is widely regarded as the most stable procedure in orthognathic surgery, requiring minimal instruments for stabilization. Rigid fixation is considered the ideal method for long-term stability, leading to improved anatomic structures, enhanced recovery of bite forces, and better bite function. Objective: To compare the skeletal stability following Le Fort I and anterior maxillary osteotomy with wire osteosynthesis and rigid fixation. Materials and Methods: A total of 16 patients, including one male and 15 females, underwent Le Fort I and anterior segmental maxillary osteotomy along with fixation using either wire osteosynthesis or rigid fixation for correction of vertical and anteroposterior maxillary excess. Patients were assessed at preoperative, 1-week postoperative, and 6-month postoperative stages using a lateral cephalogram to evaluate skeletal stability. Results: The wire osteosynthesis group showed significant differences in posterior maxillary vertical measurements compared to the rigid fixation group. However, there was no significant difference in anterior vertical and anteroposterior skeletal measurements between the two groups. Conclusion: Wire osteosynthesis is a stable form of fixation for superior repositioning with segmentation of the maxilla for correction of vertical and antero-posterior excess.

Efficient narrowband yellow organic light-emitting diodes based on iridium(III) complexes with the rigid indolo[3,2,1-jk]carbazole unit.

Phosphorescent material with narrowband emission is crucial for advancing wide-color-gamut organic light-emitting diodes (OLEDs). In this work, two iridium(III) complexes, (PhthzICz)2Ir(tmd) and (thzICz)2Ir(tmd), using rigid 2-(benzothiazole-2-yl)indolo[3,2,1-jk]carbazole (PhthzICz) and 2-(thiazole-2-yl)indolo[3,2,1-jk]carbazole (thzICz) as cyclometalated ligands and 2,2,6,6-tetramethyl-3,5-heptanedione (tmd) as ancillary ligands, were synthesized. When these complexes were doped into the host material 3,3'-di(9H-carbazol-9-yl)-1,1'-biphenyl, the doped films exhibited yellow photoluminescence (PL) peaking at 537 and 531 nm, full width at half maximum (FWHM) bands of 35 and 60 nm, and PL quantum yields of 89.9% and 85.9%, respectively. OLEDs based on these two emitters display moderate performance characteristics with maximum external quantum efficiencies of 25.2% and 22.7%. Notably, the device based on (PhthzICz)2Ir(tmd) exhibits a narrow FWHM of 31 nm. Overall, the study highlights the practicality of incorporating rigid groups into the cyclometalated ligands of Ir(III) complexes as a viable strategy for achieving efficient Ir(III) complexes for OLEDs with narrow emission and high efficiency.

Arithmetics of homogeneous spaces over p$p$‐adic function fields

AbstractLet be the function field of a smooth projective geometrically integral curve over a finite extension of . Following the works of Harari, Scheiderer, Szamuely, Izquierdo, and Tian, we study the local–global and weak approximation problems for homogeneous spaces of with geometric stabilizers extension of a group of multiplicative type by a unipotent group. The tools used are arithmetic (local and global) duality theorems in Galois cohomology, in combination with techniques similar to those used by Harari, Szamuely, Colliot‐Thélène, Sansuc, and Skorobogatov. As a consequence, we show that any finite abelian group is a Galois group over , rediscovering the positive answer to the abelian case of the inverse Galois problem over . In the case where the curve is defined over a higher dimensional local field instead of a finite extension of , coarser results are also given.

Polyampholyte-grafted silica nanoparticles for shale plugging under high-temperature and extreme-salinity conditions

Nano-/submicron-scale plugging is critical for wellbore stability during shale gas exploitation but typically fails under high-temperature and extreme-salinity conditions due to colloidal instability. Herein, polyampholyte polymers (ZP) were grafted from the surface of silicon dioxide (SiO2) nanoparticles through surface-triggered free radical polymerization to generate nanoplugging additives (SiO2-g-ZP). SiO2-g-ZP exhibited excellent colloidal dispersion stability with a nano/submicron size distribution in 35 w/v% sodium chloride (NaCl) and 11 w/v% calcium chloride (CaCl2) brines at 160 °C for a long time and over a wide pH range, and efficiently plugged 220 nm and ∼20 μm pores and 5 μm fractures at 160 °C and 3.5–5.5 MPa. Three critical aspects of observation and analysis obtained through microstructure analysis and molecular dynamics (MD) simulations helped elucidate the mechanisms that control the SiO2-g-ZP's performances: an increase in SiO2-g-ZP's hydration capability with salinity due to the antipolyelectrolyte behaviours of ZP coatings with zwitterionic nature, an efficient mitigation of Na-bent agglomeration even at harsh conditons due to the preferential adsorption of SiO2-g-ZP rather than Na+, Ca2+ and Cl− on Na-bent, and the presence of a nanosilica core and rigid groups and massive hydratable groups. The first two aspects caused a perfect matching of particles in muds to pores and fractures in formation and the third aspect helped enhance the SiO2-g-ZP's thermal resistance and residence in the pores and fractures. This work provides a novel approach that can be broadly generalized to address the thermal- and salt-tolerance challenges of colloidal systems used in the exploration and development of deep and complex shale formations.

Preparation and performance evaluation of PDDSA-MS composite as high temperature and high salinity filtration additive

Drilling operations in deep and ultra-deep wells encounter challenges arising from high temperature and saltwater intrusion, leading to reduced drilling efficiency and deteriorated performance of water-based drilling fluids. In order to alleviate the problem of increased filtration of drilling fluid under harsh environment, the composite (PDDSA-MS) was prepared by free radical polymerization using N, N-dimethyl acrylamide (DMAA), Dimethyl diallyl ammonium chloride (DMDAAC), Sodium styrene sulfonate (SSS), 2-acrylamido-2-methylpropanesulfonic acid (AMPS) and KH570 modified SiO2 as raw materials. This composite contained absorption groups, hydration groups, rigid groups and nanomaterials. Through rigorous performance evaluations, it was observed that the addition of 1 wt% composite to a fresh water drilling fluid, resulting in a remarkably low filtration of 8.8 mL after aging at 260 °C. Furthermore, when 3 wt% composite was added to the 15 wt% NaCl salt water drilling fluid, the filtration was 8 mL after aging at 220 °C. This was attributed to the particle size of the composite and its ability to maintain the dispersion stability of drilling fluid, decelerate bentonite agglomeration, and make the surface of the formed filter cake dense and smooth. These findings demonstrated the thermal stability exhibited by composite, offering an effective solution for drilling operations in deep and ultra-deep wells.

Rigid crosslink improves the surface area and porosity of β-cyclodextrin beads for enhanced adsorption of flavonoids

Many reported β-cyclodextrin (β-CD) polymers have poor flavonoid adsorption performance due to their low surface area and porosity resulting from the compact stack of the β-CD molecules crosslinked by flexible crosslinkers. Here, we propose a rigid crosslink strategy that uses phytic acid (PA) having rigid cyclic group as crosslinkers, achieving a high-surface-area (61.42–140.23 m2/g) and porous β-CD beads. The improved surface area and porosity are attributed to the rigid cyclic groups in PA, which expand the network structure of β-CD polymers. Benefitting from the advantages, the optimized PA-crosslinked β-CD (PA-β-CD) beads have an over tenfold increased adsorption amount and an threefold increased diffusivity for rutin compared with traditional non-porous β-CD beads crosslinked by epichlorohydrin. Moreover, dynamic adsorption experiments reveal that PA-β-CD beads are able to treat about 1100 mL of rutin solution (0.05 mg/mL), over 5 times higher than that of the non-porous β-CD beads (200 mL). These results demonstrate the promise of PA-β-CD beads for rapid and high-capacity adsorption of rutin.

Effect of abutment screw-access hole on the fatigue performance of implant-supported lithium-disilicate luted simplified restorations

The present study aimed to evaluate the impact of the existence of an abutment screw-access hole and the filling effects on the fatigue mechanical behavior of a luted lithium-disilicate glass-ceramic. Seventy-two discs (Ø = 10 mm, 1.0 mm in thickness) of lithium disilicate (IPS e.max CAD, Ivoclar AG) were obtained from prefabricated blocks. Thirty-six abutment specimens of an opaque zirconia (Yz - IPS e.max ZirCAD, Ivoclar AG) and titanium (Ti - Luminesse Ti-Cam discs, Talladium Inc.) were confectioned, and allocated according to 6 groups: Yz and Ti rigid (without screw access hole); Yz unfilled, Yz filled, Ti unfilled and Ti filled (with the screw access (Ø = 2.5 mm) in the center). For the unfilled groups, only a polytetrafluoroethylene tape was used. Resin composite (Tetric N-Ceram, Ivoclar AG) was applied to the screw access hole for the filled groups (Yz and Ti). A cyclic fatigue test was carried out (load of 200 N, 10,000 cycles each; 20 Hz of frequency, step size of 100 N until failure detection (radial/cone crack). The fatigue failure load (FFL) and number of cycles until failure (CFF) were recorded for statistical purposes. The stress distribution (MPa) was evaluated by finite element analysis. A statistically positive effect of the abutment material and the presence of the screw access hole was observed (p ≤ 0.05). The rigid groups (without screw access holes) depicted almost 100% of survival after the fatigue tests. Among the other groups, the Yz-filled group showed the best performance (p ≤ 0.05), followed by the Yz unfilled group. The Ti groups depicted lower values of FFL and CFF, with the Ti unfilled group showing the most unfavorable fatigue behavior (p ≤ 0.05). The lowest tensile stress concentration in the restorative material was observed with the use of rigid abutments, the filled groups depicted intermediate values, while unfilled groups showed the highest stress concentration (Yz rigid = 306.3 MPa; Ti rigid = 310.4 MPa < Yz filled = 490.7 MPa; Ti filled = 498.9 MPa < Yz unfilled = 707.6 MPa; Ti unfilled = 719.7 MPa). Therefore, the presence of a screw-access hole decreases the mechanical performance of a lithium disilicate ceramic regardless of the abutment material. In the presence of a screw-access hole, zirconia abutments depicted a higher fatigue failure load when compared with titanium. The filling of the abutment screw-access hole with resin composite increased the mechanical performance of the simulated restoration.

Remarks on the inverse Galois problem over function fields

In this paper, we prove new instances of the inverse Galois problem over global function fields for finite groups of Lie type. This is done by constructing compatible systems of ℓ-adic Galois representations valued in a semisimple group G using Galois theoretic and automorphic methods, and then proving that the Galois images are maximal for a set of primes of positive density using a classical result of Larsen on Galois images for compatible systems.

Synthesis and performance of novel fluoro acrylate polymer containing rigid cyclohexane group as a mesogenic core

Fluorine-containing liquid crystal polymers have excellent hydrophobic stability, making them attractive for use in the field of stable hydrophobic coatings. To improve the hydrophobic stability of fluoropolymers, a novel fluoro acrylate monomer (FAM) containing a rigid cyclohexane structure was simply synthesized using isophorone diisocyanate (IPDI), hydroxyethyl methacrylate (HEMA), and perfluorohexylethanol (S104) as raw materials and its homopolymer (PFAM) was prepared by radical solution polymerization. The structure, liquid crystal behavior, thermal properties, hydrophobic properties, and surface properties of FAM or PFAM and a reference substance, dodecafluoroheptyl methacrylate (DFMA) and its homopolymer (PDFMA), were studied using various techniques including FT-IR, NMR, GPC, XRD, POM, DSC, TGA, XPS, AFM, and static/dynamic contact angle measurements. The results indicate that FAM and PFAM are thermally liquid crystalline. PFAM's thermal stability is higher than that of PDFMA. The presence of a rigid cyclohexane structure facilitates the dense distribution of fluorocarbon chain tails of the homopolymer and also prevents surface reconstruction when the homopolymer comes into contact with polar liquids, resulting in improved hydrophobicity and hydrophobic stability. Thus, this study provides a straightforward method for the synthesis of fluoro acrylates with surface stability, highlighting their potential application in hydrophobic coatings.

Surface Houghton groups

AbstractFor every $$n\ge 2$$ n ≥ 2 , the surface Houghton group$${\mathcal {B}}_n$$ B n is defined as the asymptotically rigid mapping class group of a surface with exactly n ends, all of them non-planar. The groups $${\mathcal {B}}_n$$ B n are analogous to, and in fact contain, the braided Houghton groups. These groups also arise naturally in topology: every monodromy homeomorphism of a fibered component of a depth-1 foliation of closed 3-manifold is conjugate into some $${\mathcal {B}}_n$$ B n . As countable mapping class groups of infinite type surfaces, the groups $$\mathcal {B}_n$$ B n lie somewhere between classical mapping class groups and big mapping class groups. We initiate the study of surface Houghton groups proving, among other things, that $$\mathcal {B}_n$$ B n is of type $$\text {F}_{n-1}$$ F n - 1 , but not of type $$\text {FP}_{n}$$ FP n , analogous to the braided Houghton groups.

A strategy to prepare low-temperature curable photosensitive polyimide with good comprehensive performance

Low-temperature curable photosensitive polyimide (PSPI) materials have attracted widespread attention in advanced packaging, serving as key insulating dielectric materials in redistribution layer (RDL) to avoid wafer warpage. Considering PSPI and copper together formed the core RDL part, the adhesion between PI and copper need to be concerned to realize high reliability. However, the exploration of novel diamines with desirable characteristics such as low temperature curing ability, improved adhesion and excellent lithography properties was limited. We designed a new diamine with fluorine-containing pyridine side group named PyFNH2, which facilitated preparation of low-temperature curable polyimide (at 200 °C) with remarkable degree of imidization (≥95.9 %). Secondly, the coordination interaction between pyridine side group and copper enhanced adhesion strength. Thirdly, the PI films cured at 200 °C exhibited favorable dielectric properties compared to control films without PyFNH2, because of the introduction of electronegative fluorine atoms and rigid side groups. Apart of these, the PI films possessed fairly good thermal and mechanical properties. Significantly, negative-tone PSPI employing the precursor poly (amic ester) with PyFNH2 was successfully prepared and cured at 200 °C, enabling precise lithography patterns with line resolutions down to 3 µm. Our findings highlight promising application potential of PyFNH2 in photolithography technology, offering a feasible strategy for structure designs of low-temperature curable diamine.

Construction of an epoxidized, phosphorus-based poly(styrene butadiene styrene) and its application in high-performance epoxy resin

In modern industries, the demand of multifunctional, transparent epoxy resin (EP) combining superior dielectric, mechanical, and fire-safety performances is gradually increasing. However, it was difficult to realize such a performance portfolio in current research. Herein, we fabricated an epoxidized, phosphaphenanthrene-containing poly(styrene butadiene styrene) (ESD) for advanced fire-safe EP. ESD maintains the high transparency of EP and improves the UV-blocking property. 10 wt% ESD makes EP/DDM/10ESD pass a limiting oxygen index (LOI) of 36.0 % and a UL-94 V-0 rating. EP/DDM/10ESD displays improved mechanical properties because of intramolecular cavities and rigid P-based groups in ESD, as reflected by 79.2 %, 68.9 %, and 67.6 % increases in impact strength, tensile strength, and fracture toughness (KIC) relative to EP/DDM. Compared with EP/DDM, EP/DDM/10ESD shows obviously-decreased dielectric constant and loss. EP/DDM/10ESD outperforms many fire-safe epoxy resins due to its superior comprehensive performances. Thus, this work delivers an effective method for developing multifunctional flame-retardant epoxy resin.

Effects of rigid end-capped groups on the structure and properties of colorless polyamide-imide films

In this work, we use 2-aminobenzimidazole and 2-aminobenzoxazole to end-cap colorless polymer, PAITFDB-TPC-6FDA, in order to investigate methods for reducing the CTE value of products and their effects on other properties. Results show that, as the ratio of 2-aminobenzimidazole to the raw material, diamine TFDB, increases from 0:100 to 2:99, the CTE value of products gradually decreases from 20.0 to 12.9 ppm/K (a decrease of 36%). Nevertheless, the CTE value of products rebounds as the dosage of the end-capped groups exceeds this ratio. Among all the products obtained, PAI-1M film has the best overall properties: its CTE is 12.9 ppm/K, Td5 is 518 ℃, Tg is 366 ℃, TS is 191 MPa, T430 is 85.1%, WA is 85.4% and CA is 0.8%. The two end-capped groups are of highly linear and rigid structures. They also help to enhance the hydrogen bonding between molecules, resulting in a decrease in d-spacing values. The results of molecular configuration simulation and calculation indicate that, using 2-aminobenzimidazole to end-cap the chains of PAI molecules is beneficial for suppressing the formation of the charge-transfer complexes (CTCs), and thereby offsetting impacts of the reduced molecular chain spacing on the transparency of films. Based on these two influencing factors, we confidently conclude that linear and rigid end-capped groups can effectively improve the dimensional stability and mechanical properties of PAI films without damaging their optical properties.

An oil-absorbing resin with a simple polymerization system with benzyl methacrylate as a functional monomer.

To solve the problem of the low absorbency of oil-absorbing resins, oil-absorbing resins (PAMs) were fabricated in this study by introducing commercially available benzyl methacrylate (BZMA) as a functional monomer copolymerized with stearyl methacrylate (SMA) and butyl acrylate (BA). The internal network structure of the PAMs expanded more easily when absorbing oils or organic solvents after introducing rigid groups of the benzene ring by an uncomplex polymerization process, which provided the oil-absorbing resin with good absorbency. The reagents were all commercially available, and there was no other pretreatment or posttreatment process. Then, the optimum parameters for the monomer feed ratio, water/oil mass ratio, and concentrations of initiator, stabilizer and crosslinker were studied. Simultaneously, the reusability, oil retention and thermal stability of PAMs were investigated in this article. The PAMs swelled in various oils and organic solvents (the values of oil absorbency were 44.52, 56.13, 25.54, 28.21, 32.85, 24.56, 14.17, 15.02 and 29.07 g g-1 for CCl4, CHCl3, CH2Cl2, benzene, toluene, xylene, n-hexane, 0# diesel oil and 93# gasoline, respectively) and displayed good oil absorbency, which met the absorption requirements for common oils or organic solvents.

Flexible films of main-chain luminescent liquid crystalline polymers containing renewable biobased bifuran moieties

Luminescent liquid crystalline polymers (LLCPs) have attracted great attention due to their potential applications in liquid crystal display, optical memory storage, organic light-emitting diode, and other fields. However, the fluorescence signals of LLCPs bearing conventional luminophores showing aggregation-caused quenching behavior are strikingly diminished or even vanish in the aggregated state. Herein, we have synthesized three main-chain LLCPs with α-cyanostilbene (CS) and bifuran units connected by different flexible spacer lengths via melt polycondensation. On the one hand, the CS unit acts as a rigid rod-like mesogen with aggregation-induced emission (AIE) characteristics. On the other hand, the renewable biobased bifuran moiety has been chosen because of two main reasons. One is it is a perfect alternative to the rigid fossil-based biphenyl group. The other reason is the addition of bifuran moiety is beneficial for the polycondensation and the extension of the chain length, leading to the enhancement of the toughness of LLCPs. Upon 365 nm UV light irradiation, these polymers undergo an irreversible Z/E isomerization in solution, while the [2 + 2] cycloaddition of CS chromophores occurs in the solid film. Importantly, the polymers display a good film-forming ability, so that the flexible thin films can be achieved by ordinary compression molding. Moreover, the fluorescent patterns can be directly printed on the flexible films on the basis of the [2 + 2] cycloaddition behavior, which shows their promising applications ranging from information storage to high-tech anti-counterfeiting.

Reaction mechanism investigation of low Df epoxy-active ester film composites for 10 GHz high-frequency communications

The role of electronic-grade thin film composite materials in packaging circuits (integrated circuits) and printed circuit boards is critical. Developing epoxy-based thin film composite materials with low dielectric loss (Df) is a significant challenge, especially for that used in high frequency communication (eg. 10 GHz). Active esters, as excellent curing agents, do not form strongly polar hydroxyl groups upon reacting with epoxy resins, which offers significant advantages for low Df thin film composites. In this study, a low Df and high thermal resistance thin film composites was prepared by selecting epoxy resins with biphenyl structures and active esters with dicyclopentadiene groups. The introduction of rigid groups reduces the motility of the molecular chains, and its electron conjugation effect weakens the impact of an external electric field on the molecular dipole moment. The essence of low Df is explained by DFT calculations, in which the quenching effect of active esters on oxygen anions reduces the polarity of the molecular. Meanwhile, ensuring sufficient reaction at a sufficiently high temperature can reduce the number of residual free molecule fragments and ensure the integrity of the cross-link network. This work provides a new direction for the design of the next generation of high-performance low Df composites by deeply explaining the nature of active esters in reducing dielectric loss.

PDD-ET: Parkinson’s Disease Detection Using ML Ensemble Techniques and Customized Big Dataset

Parkinson’s disease (PD) is a neurological disorder affecting the nerve cells. PD gives rise to various neurological conditions, including gradual reduction in movement speed, tremors, limb stiffness, and alterations in walking patterns. Identifying Parkinson’s disease in its initial phases is crucial to preserving the well-being of those afflicted. However, accurately identifying PD in its early phases is intricate due to the aging population. Therefore, in this paper, we harnessed machine learning-based ensemble methodologies and focused on the premotor stage of PD to create a precise and reliable early-stage PD detection model named PDD-ET. We compiled a tailored, extensive dataset encompassing patient mobility, medication habits, prior medical history, rigidity, gender, and age group. The PDD-ET model amalgamates the outcomes of various ML techniques, resulting in an impressive 97.52% accuracy in early-stage PD detection. Furthermore, the PDD-ET model effectively distinguishes between multiple stages of PD and accurately categorizes the severity levels of patients affected by PD. The evaluation findings demonstrate that the PDD-ET model outperforms the SVR, CNN, Stacked LSTM, LSTM, GRU, Alex Net, [Decision Tree, RF, and SVR], Deep Neural Network, HOG, Quantum ReLU Activator, Improved KNN, Adaptive Boosting, RF, and Deep Learning Model techniques by the approximate margins of 37%, 30%, 20%, 27%, 25%, 18%, 19%, 27%, 25%, 23%, 45%, 40%, 42%, and 16%, respectively.

Evaluating rearing behaviour as a model-specific pain indicator in mouse osteotomy models.

To assess pain in mouse models of bone fractures, currently applied assessment batteries use combinations of clinical signs with spontaneous behaviours and model-specific behaviours, including walking and weight-bearing behaviour. Rearing behaviour - an upright position on the hindlimbs - has a motivational and an ambulatory component. Thus, rearing behaviour might have the potential to be an indicator for model-specific pain in mouse fracture models. To date, the assessment of rearing behaviour in bone fracture models using mice is only scarcely described. In this study, we aimed to determine whether the duration of rearing behaviour is affected by osteotomy of the femur in male and female C57BL/6N mice with external fixation (rigid vs. flexible) and could be an additional sign for model-specific pain, such as the presence of limping. Rearing duration was significantly decreased after osteotomy in male and female mice at 24 h, 48 h and 72 h, but was not affected by anaesthesia/analgesia alone. In male mice, the relative rearing duration increased over 72 h (both fixations) and at 10 days in the rigid fixation group but remained significantly lower in the flexible fixation group. In contrast, in female mice, no increase in rearing duration was observed within 72 h and at 10 days post-osteotomy, independent of the fixation. We did not identify any association between relative rearing time and presence or absence of limping. In summary, our results do not provide sufficient evidence that altered rearing behaviour might be an indicative sign for pain in this model.