The purpose of this article is to investigate the changes in the magnitude of natural frequencies and their associated modal shapes of Timoshenko beam with respect to different system design parameters. This beam includes an intermediate extended eccentric rigid mass mounted on two elastic segments. The equilibrium equations which govern the transverse and rotational motions are derived. The application of the developed system frequency equation is demonstrated by several illustrative examples. Several end and intermediate conditions are considered. The influence of, rotary inertia, shear deformation, axial load, eccentric mass and elastic segments step ratio on the system natural frequencies and mode shapes are conducted. Several sets of new results are presented. Comparison of the present model results with the experimental data for shaft integrated with intermediate rigid mass demonstrates the accuracy of the analysis in practical applications. The present model is valid for several industrial applications, such as mechanical, structural, naval and for wider range of applications.