Rich Content Research Articles

Continuous step-wise temperature extraction improves the flavanol content of tea beverages.

Tea is the second most consumed beverage worldwide and its health benefits have been extensively studied because of its rich flavanol content. This study aims to evaluate the efficacy of a novel continuous step-wise temperature extraction process in maximising antioxidant activity, total phenolic content and flavanol concentration of different tea beverages. Continuous step-wise temperature extraction produced the highest absolute antioxidant capacity, total phenolic content and concentration of major tea flavanols in Yuhua tea compared to the other tea types at all extraction points. Despite having the lowest baseline concentration of tea flavanols, Lapsang Souchong tea showed the greatest increase in catechin and epigallocatechin-3-gallate levels with the continuous step-down temperature process, by over 500% and 300%, respectively. In addition, Moonlight White tea showed the greatest percentage change in total antioxidant capacity and phenolic content at the end of the process compared to the baseline. Furthermore, the continuous step-wise temperature extraction showed a moderate increase in caffeine concentration in all the tea samples. Our findings revealed a beneficial impact of continuous step-wise temperature extraction on the antioxidant capacity, phenolic content and flavanol profile in different types of tea, which may be valuable in the production of added-value tea beverages. © 2024 Society of Chemical Industry.

Application of QuEChERS extraction and LC-MS/MS for determination of pharmaceuticals in sewage sludges sampled across the Czech Republic.

The use of pharmaceuticals entails a significant risk of environmental contamination. Wastewater treatment plants (WWTPs) are considered to be the main contributors to contamination as they ineffectively eliminate these compounds from wastewater. Simultaneously, they produce solid waste, sludge, which often contains a variety of retained pollutants, including pharmaceuticals. Since sewage sludge is frequently applied to agricultural soil due to its rich nutrient content, pollutants are introduced into the environment in this way. Only a few studies have been carried out on the topic of the analysis of pharmaceuticals in sludge. Therefore, information on the occurrence of pharmaceuticals in sludge is limited. The present study employed quick, easy, cheap, effective, rugged and safe (QuEChERS) extraction and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis to establish a simple and reliable procedure for determining 16 pharmaceuticals (antibiotics, anticonvulsants, antidepressants and β-blockers) in sewage sludge. The method has been thoroughly validated, and parameters such as linear range, accuracy, precision, matrix effects and detection and quantification limits were assessed. Our method achieved low limits of quantification (0.5-9.0µgkg-1) and satisfactory recoveries (51-101%). Forty sludge samples from different WWTPs across the Czech Republic were analysed. Fourteen compounds were detected and quantified in most samples, with antidepressants having the highest detection frequency and overall content. Sertraline, with a mean concentration of 521.0µgkg-1, was notably prevalent alongside its metabolite norsertraline (mean concentration 204.9µgkg-1). The antibiotic azithromycin was also found at higher levels (mean concentration 185.1µgkg-1).

Assessment of growth and essential oil profiling in leafy coriander (Coriandrum sativum L.) genotypes

Coriander is a versatile plant prized for its dual role as a culinary spice and a medicinal herb. Leafy coriander, an herbaceous plant, is particularly noted for its rich content of bioactive compounds, such as essential oils, flavonoids, and vitamins. The experiment was conducted at the Department of Spices and Plantation Crops, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore. A study was conducted using a Randomized Block Design (RBD) with two replications, focusing on critical growth and yield traits. Among the fifteen genotypes evaluated, the CSL1 genotype illustrates superior performance in various growth and yield parameters. Coriander leaves are extensively studied for their antioxidant, anti-inflammatory, and antimicrobial properties, underscoring their significance in nutritional and medicinal contexts. In this research, coriander leaf oil was extracted and analysed using gas chromatography-mass spectrometry (GC-MS) to determine its chemical composition. The GC-MS analysis identified 60 distinct compounds in the essential oil, with the major constituents being 3-dimethylamino acrylonitrile, trans-2-Dodecen-1-ol, Cyclooctane, Decanal, 13-Octadecenal, 9-Decen-1-yl acetate and 2-Propen-1-amine. These results offer valuable insights into the unique chemical profile of coriander leaf oil, contributing to its distinctive aroma and potential applications in flavouring fragrance and therapeutic formulations.

Effect of sintering temperature on the properties of ceramic binders synthesized from coal gangue-based geopolymer

ABSTRACT As a by-product of the coal industry, the effective use of coal gangue presents significant implications for environmental protection and sustainable development upon effective utilization. Given the rich content of silicon and aluminum in coal gangue, this study converts it into a precursor for ceramic binders used in grinding wheels through the geopolymerization reaction process. Ceramic binders of the SiO2-B2O3-Al2O3-RO-R2O system were prepared at sintering temperatures ranging from 590 to 740°C, and the performance of each sample was tested. The research found that the flexural strength of the ceramic binder reached a peak of 31 MPa when sintered at 690°C. Additionally, the hardness was observed to be the highest at a sintering temperature of 640°C, reaching 211.8 hV. It was observed that as the sintering temperature rose, wettability tended to decrease. Using techniques including XRD, SEM, and EDS, the effect of sintering temperature on the microstructure and phase transformation of the material was analyzed, and these changes determined the properties of the material. This study not only confirmed the potential value of coal gangue in the abrasive tools industry but also opened up new strategies and technical solutions for the field of solid waste resource utilization.

Effect of Extracted Walnut Pomace Cake Edible Coating and Packaging Type on Quality Parameters of Walnut (Juglans regia L.) Kernels During Long Storage Periods

Walnuts are highly valued for their rich nutritional content, but their quality degrades during storage due to oxidation and other quality reducing processes. This study investigated the use of bioactive compounds extracted from walnut pomace, a by-product of walnut oil production, to develop an edible coating that extends the shelf life of ‘Milotai 10’ walnut kernels. Walnut kernels were stored for eight months in four different packaging materials (PE, PP, MPP, PLA) and three treatments were used (air or without treatment, nitrogen gas, and walnut pomace extract coating). The effects of these combinations on walnut quality parameters, including polyphenol content, vitamin E, fatty acids, peroxide value, and color, were analyzed. The results show that the combination of metallized polypropylene packaging under nitrogen treatment or with atmospheric air and walnut pomace extract (MPP-A-E+) best preserves walnut quality, making it a promising option for extending shelf-life.

Effects of fish meal replacement with composite proteins source on growth performance, antioxidant capacity and swim bladder quality of chu’s croaker (Nibea coibor)

Collagen, as a commercial functional protein, is widely applied in food, cosmetics, biomedicine and other industries, and swim bladder is regarded as an ideal source of raw materials due to its rich collagen content. Chu’s croaker (Nibea coibor), as an economical fish that can produce high-grade swim bladder, is popularly farmed, however, its high fishmeal demand limits the development of the feed industry. Therefore, it is worth thinking about how to reduce the use of fish meal without affecting the swim bladder quality. Given that, an 8-week feeding trial was conducted to investigate the effects of fish meal partially replaced (0.0%, 5.0%, 10.0%, 15.0%, 20.0%) with composite proteins (CPs, consisted of corn protein meal and hydrolyzed feather meal) on growth, antioxidant capacity and swim bladder quality (texture and collagen deposition) of chu’s croaker. Results showed that although growth performance decrease with the increase of CPs’ replacement level, it did not compromise the swim bladder quality. Specifically, 5% - 10% CPs did not affect the growth significantly, but can improve antioxidant capacity, protein content, collagen synthesis and texture quality of swim bladder. The enhancement of swim bladder texture, including hardness, chewiness, springiness, and adhesiveness may be caused by changes in the expression of related genes, such as colga1t1, col12a1, cyp27a1, etc. These results indicated that appropriate fish meal replaced by CPs could be applied during aquaculture practice after comprehensive consideration of feed cost, growth rate and quality of swim bladder. This study provides support for the development of cost-effective feed formulations that improve the sustainability and economic viability of chu’s croaker culture.

Brassica oleracea var italica and Their By-Products as Source of Bioactive Compounds and Food Applications in Bakery Products.

Broccoli (Brassica oleracea var. italica) is one of the most consumed cruciferous crops in the world, with China and Spain acting as the main producers from outside and within the EU, respectively. Broccoli florets are edible, while the leaves and stalks, discarded in the field and during processing, are by-products. Therefore, the objective of this study was to conduct a comprehensive review of the nutrient and phytochemical composition of broccoli and its by-products, as well as its beneficial effects. In addition, the study highlights the revalorization of broccoli by-products through innovative green technologies and explores their potential use in bakery products for the development of functional foods. The studies suggested that broccoli is characterized by a high content of nutrients and bioactive compounds, including vitamins, fiber, glucosinolates, and phenolic compounds, and their content varied with various parts. This high content of value-added compounds gives broccoli and its various parts beneficial properties, including anti-cancer, anti-inflammatory, antioxidant, antimicrobial, metabolic disorder regulatory, and neuroprotective effects. Furthermore, broccoli and its by-products can play a key role in food applications by improving the nutritional profile of products due to their rich content of bioactive compounds. As a result, it is essential to harness the potential of the broccoli and its by-products that are generated during its processing through an appropriate agro-industrial revalorization, using environmentally friendly techniques.

Aframomum Melegueta: Evaluation of Chronic Toxicity, HPLC Profiling, and In Vitro/In Vivo Antioxidant Assessment of Seeds Extracts.

Aframomum melegueta, commonly known as grains of paradise, is a medicinal plant celebrated for its rich phytochemical content and therapeutic properties. This study evaluated the antioxidant and cytotoxic potentials of its ethanolic and methanolic extracts, both in vitro and in vivo, while also analyzing their chemical profiles. HPLC analysis identified key compounds, including gallic acid, caffeic acid, caffeine, coumarin, rutin, catechin, ferulic acid, and quercetin. Chronic toxicity assessments confirmed the safety of the extracts, with no adverse effects on animal health, particularly in liver histopathology. Cytotoxicity results indicated reduced splenocyte viability at the highest concentrations. The extracts exhibited significant antioxidant activity in DPPH•, FRP, and phosphomolybdate assays, demonstrating their effectiveness as antiradical agents. In vivo antioxidant results showed a reduction in lipid peroxidation levels in serum and liver, highlighting the extracts' ability to mitigate oxidative stress. Additionally, the extracts provided protection against H2O2-induced erythrocyte hemolysis and modulated NO production in peritoneal macrophages. These findings underscore the therapeutic potential of A. melegueta extracts, suggesting their promise in developing preventive strategies for oxidative stress-related chronic diseases.

Teff: Its applicability in various food uses and potential as a key component in gluten-free food formulations—An overview of existing literature research

In recent years, there has been a significant increase in demand for gluten-free foods. This rise can be attributed to a growing number of individuals opting for gluten-free diets due to factors such as gluten sensitivity and celiac disease. Interest in teff has also increased markedly due to its high protein content, balanced amino acid composition, and rich nutrient contents including fiber, iron, and calcium, along with its gluten-free nature. Additionally, teff exhibits positive functional properties that contribute to improving texture, moisture retention, and shelf life in gluten-free products. Its mild flavor profile allows for versatile applications across a spectrum of culinary applications, enhancing the sensory appeal and taste of gluten-free offerings. This makes it a suitable alternative to wheat and other grains in food applications for individuals with celiac disease. Overall, integrating teff into gluten-free formulations presents an attractive opportunity to enhance the nutritional quality, sensory experience, and market competitiveness of gluten-free products, catering to the diverse needs and preferences of consumers seeking gluten-free alternatives for health, dietary, or lifestyle reasons. Further research and innovation in teff-based product development are crucial to fully unlock its potential and promote inclusive, sustainable growth in the gluten-free food sector. Therefore, these attributes underscore teff's promising role in the global food and beverage industries.

Phenolic Profiling, In Vitro Antiglycation, Antioxidant Activities, and Antidiabetic Effect of Algerian Trigonella Foenum-Graecum L. in Rats Administered a β-Cell Toxicant.

This study sought to quantitatively assess individual and total polyphenols, mineral composition, antioxidant and antiglycation activities of Algerian fenugreek seeds (AFS) as well as the antidiabetic effect of its supplementation on streptozotocin-induced diabetic rats. Forty rats were divided into four groups (i) non diabetic rats, (ii) non diabetic rats +10 % AFS, (iii) diabetic rats, (iv) diabetic rats +10 % AFS. Flame-SAA analysis revealed a rich content in micro-elements, HPLC DAD-FLD analysis revealed twenty components with rutin and ferulic acid being the major compounds in AFS hydro-methanolic extract while spectrophotometric assays scrutinized moderate contents in total phenolics and flavonoids. The extract was potent in scavenging ABTS⋅+ and DPPH+ (42.06±2.14 and 55.84±4.14 mg TE/g), reducing Fe3+ and Mo6+ (35.12±2.45 and 29.89±3.12 mg TE/g) and inhibiting AGEs (IC50=1.03±0.02 mg/ml). In vivo, 10 %AFS- supplemented diet (w/w) was found to elicit a significant reduction in glycemia (66.74 %), TNF α (9.4 %), IL-6 (23.74 %), CRP (31.10 %), liver enzymes, lipid peroxidation (MDA) (47.24 %;30 %), protein carbonyl (PCO) (28.35 %; 27.15 %), improvement in insulin level (79.74 %), reduced glutathione amount (GSH) (41.01 %; 16.55 %), glutathione peroxidase (GPx) (45.80 %; 56.37 %), catalase (CAT) (24.44 %; 35.42 %) and glutathione-S-transferase (GST) (22.78 %; 22.90 %) activities, in liver and pancreas respectively, along with a rejuvenation of hepatic and pancreatic histological features. These outcomes disclosed that AFS is endowed with biologically effective components which could be decent applicant to attain the objective of mitigating glycation, oxidative stress and diabetes-related complications.

Plantago boissieri : Phytochemical Assessment, Antioxidant, Anti-inflammatoryand Wound Healing Potential.

Plantago is a large genus under family Plantaginaceae. Plantago species were noted for potent antioxidant, anti-inflammatory and wound healing activities. The current research investigated the potential bioactivities as the metabolic content of Plantagoboisserei extract. Results highlighted the rich content of phenolics (450.93 ± 7.4 mg GAE/g extract) and flavonoids (144.2 ± 3.6 mg RE/g extract). HPLC analysis enabled the detection of 17 phenolic constituents among which ellagic acid was found in highest concentration followed by rutin. P. boisserei exhibited a potent antioxidant activity evidenced by the IC50 values in ABTS and H2O2 assays (10.95 and 10.87 µg/mL, respectively) as well as in TAC assay (67.94 mg AAE/g). The anti-microbial activities revealed a moderate activity against Staphylococcus aureus and Proteus vulgaris. In vitro anti-inflammatory potential indicated a characteristic inhibition against COX-1 and COX-2 enzymes with IC50 62.8 and 14.23 µg/mL, respectively, compared to ibuprofen (IC50 8.07 and 6.58, respectively). Additionally, P. boisserei extract achieved a potent wound healing activity using in vivo rat model, this might be attributed to its high content of flavonoids together with other polyphenolic compounds that have a great free radical scavenging potential. P. boisserei is a promising candidate for more extensive phytochemical and biological exploration.

Acceptability of Bangus-Clam Sisig

Milkfish is one of the healthy and nutritious fish available, and it is loaded with omega-3 fatty acids and protein, which are essential for the proper functioning of the body. Mud clams, on the other hand, are rich in Vitamin B12, providing iron and additional protein. Mud clams, with their potential to regulate high blood pressure and support thyroid health, are a promising addition to any diet. Their rich content of riboflavin, choline, minerals, and vitamins, coupled with their low-fat content, make them a healthy choice. This study utilized bangus flakes and clam meat in varying proportions, combined with butter, garlic, chili, onion, liver spread, soy sauce, black pepper, annatto oil, oil, and salt. The objectives of the study were to develop a bangus-mud clam sisig product, evaluate its sensory characteristics (color, odor, taste, and texture) in different formulations, and determine whether there were significant differences in the acceptability of bangus-clam sisig across treatments. Moreover, the study aimed to identify the most preferred formulation (MPF) and assess consumer preferences. The development was performed in five (5) treatments with three (3) replications. The quality of the product was determined through sensory evaluation. The bangus-clam sisig had similar color, odor, taste, texture, and acceptability, regardless of the amount of bangus flakes and mud clam meat added to the formulated product. The addition of bangus and mud clam in the development of sisig, even in varying percentages, had no significant effect on the results in terms of color, odor, taste, and texture. However, during cooking, especially when pan-frying, the skin browns and crisps up. This contributed a significant amount of brown color to the overall dish. The results show that there was no significant difference in the overall acceptability of bangus-clam sisig, regardless of the amount of bangus and clam. All treatments were acceptable. Out of the five (5) treatments, the most preferred formulation consisted of 50% bangus and 50% mud clams. The findings show that most of the faculty employee and college students at the Capiz State University-Dayao Satellite College, preferred the bangus-clam sisig.

A Study on the Influencing Factors of New Media Short Video Platforms on Adolescent Mental Health

With the rapid development of the Internet and mobile technology, new media short video platforms have rapidly become popular around the world and have become an important part of adolescents ‘s daily lives. Short video platforms are popular among adolescent for their rich content and strong interactivity. However, the popularity of short video platforms also brings potential impacts on the mental health of adolescents. This study aims to discuss the specific influencing factors of new media short video platforms on adolescents’ mental health. It uses a combination of questionnaire surveys and literature searches to study factors such as the frequency of adolescents’ short video use, content preferences, and interactive behaviors The results of this study show that new media short video platforms have a multi-faceted negative impact on the mental health of adolescents, mainly manifested in increased psychological stress, decreased sleep quality, and decreased academic performance. The research further concluded that when adolescents overuse short video platforms, their academic and social pressures increase significantly. In response to this type of problem, it is recommended that parents and educators provide more guidance, use short video platforms reasonably, and create a healthy online environment.

In-Depth Phytochemical Profile by LC-MS/MS, Mineral Content by ICP-MS, and In-Vitro Antioxidant, Antidiabetic, Antiepilepsy, Anticholinergic, and Antiglaucoma Properties of Bitlis Propolis

Propolis is very significant in terms of its phytochemical content and biological activity among bee products. In this study, the antioxidant activities (total phenolic and flavonoid, Fe3+, Cu2+ (CUPRAC), Fe3+-TPTZ (FRAP) reducing, and DPPH•, ABTS•+ scavenging assays) of propolis collected from the Bitlis province of Türkiye were determined. In addition, the carbonic anhydrase I and II isoenzymes (hCA I and hCA II), α-glycosidase, acetylcholinesterase (AChE), and butyrylcholinesterase (BChE) inhibition activity and phytochemical profile of propolis and mineral content were determined by LC-MS/MS and ICP-MS, respectively. In propolis, 31 phytochemicals were found, and the highest concentration of acacetin (23.604 mg/g) was detected. It is seen that the phytochemicals in propolis provide antioxidant properties. The mineral content was screened for 18 elements and determined to be rich in the elements that make up the salt content. Total phenolic content was 215.14 mg GAE/g, and total flavonoid content was 79.11 mg QE/g. The Fe3+ reduction result was 0.940 (µg/mL), CUPRAC 1.183 (µg/mL), FRAP 0.963 (µg/mL), DPPH• scavenging IC50: 16.7 (µg/mL), and ABTS IC50: 8.01 (µg/mL). hCA I enzyme inhibition results in IC50: 7.19 (µg/mL), hCA II 8.15, AChE 5.17, BChE 7.50, and α-Glycosidase 5.72. As a result of this study, it was determined that Bitlis propolis has high antioxidant properties and a rich phytochemical content. It was also observed that it is an effective enzyme inhibitor against epilepsy, glaucoma, Alzheimer’s, and diabetes, which are important diseases, and it can be evaluated in the treatment of these diseases and drug production.

A Study on the Correlation between Danmaku Videos and Audiences’ Repetitive Viewing Behaviours - A Case Study of Bilibili Danmaku Video Network Videos

With the rise of online video platforms, Danmaku videos have steadily gained popularity among young people. Danmaku’s instantaneous, synchronous, targeted, and cumulative features offer viewers a novel viewing experience, improve the social and interactive aspects of video views, and even impact the frequency with which users watch videos again. As a popular video website at present, Bilibili provides rich video content for audiences and supports the Danmaku function. This study targets Bilibili Danmaku videos and focuses on how Danmaku videos affect the audience’s viewing motivation and experience as the research theme. Descriptive statistics and result analysis were utilized in this study to investigate the reasons behind repeat viewing and the effect of the Danmaku on the viewing experience. Questionnaires were employed to gather viewing data from video audiences in the Bilibili life division. According to the findings of the study, Danmaku greatly improves the overall viewing experience for viewers as well as their enjoyment of the show and sense of community. The study concludes that Danmaku facilitates the audience’s behavioral decisions of repetitive viewing to a certain extent. This study provides an empirical analysis to explore the role of Danmaku in video viewing behavior and makes strategic recommendations for video platforms to improve user experience and foster community engagement.

Unlocking the Potential of Teff for Sustainable, Gluten-Free Diets and Unravelling Its Production Challenges to Address Global Food and Nutrition Security: A Review.

Sustainable diets, as defined by the Food and Agriculture Organisation, aim to be nutritionally adequate, safe, and healthy, while optimising natural and human resources. Teff (Eragrostis tef), a gluten-free grain primarily grown in Ethiopia, has emerged as a key contender in this context. Widely regarded as a "supergrain", teff offers an outstanding nutrition profile, making it an excellent choice for people with gluten-related disorders. Rich with protein, essential amino acids, polyunsaturated fats, and fibre, and abundant in minerals like calcium and iron, teff rivals other popular grains like quinoa and durum wheat in promoting human health. Beyond its nutritional benefits, teff is a hardy crop that thrives in diverse climates, tolerating both drought and waterlogged conditions. Due to its resilience and rich nutrient content, teff holds the potential to address nine of the 17 United Nations' Sustainable Development Goals (SDGs), including SDG 1 (no poverty), SDG 2 (zero hunger), and SDG 3 (good health and wellbeing), which are tied to improving food and nutrition security. However, teff production in Ethiopia faces significant issues. Traditional farming practices, insufficient storage infrastructure, and food safety challenges, including adulteration, hinder teff's full potential. This review explores teff's dual role as a nutritious, sustainable food source and outlines the key challenges in its production to conclude on what needs to be done for its adoption as a golden crop to address global food and nutrition security.

Genome-Wide Identification of UGT Genes and Analysis of Their Expression Profiles During Fruit Development in Walnut (Juglans regia L.)

Walnut (Juglans regia L.) possesses the ability to prevent coronary heart disease and promote cardiovascular health. This ability can be attributed to their rich content of polyphenols, particularly flavonoids. The biosynthesis of flavonoids is reliant on the catalytic activity of uridine diphosphate glycosyltransferase (UGT). However, the identification of UGTs in walnut has not been reported. In the current study, a total of 124 UGT genes containing the PSPG box were identified from the walnut genome. Based on phylogenetic analysis, the 124 UGTs could be classified into 16 distinct groups, which exhibited an uneven distribution across the 16 chromosomes. Subcellular localization prediction analysis revealed that approximately 78.23% of walnut UGT proteins were predominantly localized in the cytoplasmic compartment. Furthermore, motif annotation confirmed that motifs 1, 2, and 3 represented conserved structural features within UGT proteins, while interestingly, around 56.5% of walnut UGT members lacked introns. Through the analysis of promoter cis-regulatory elements, it was revealed that JrUGTs are involved in photoresponse, hormonal regulation, and other physiological responses. In conjunction with transcriptome analysis and quantitative expression, approximately 39% of UGT genes in walnut exhibited high expression levels during early fruit development. Correlation analysis between UGT genes’ expression and phenolic content in walnut indicated that JrUGT6, JrUGT38, JrUGT39, JrUGT58, JrUGT69, JrUGT75, and JrUGT82 might be involved in phenolic biosynthesis in walnut. This comprehensive study provides an overview of the UGT genes in walnut, serving as a valuable reference and theoretical foundation for further investigations into the biological functions of JrUGTs in flavonoid biosynthesis.

Enhancing Entrepreneurship Education with Innovatively Designed YouTube Videos: Evaluating Student Learning and Effectiveness of Youtube Videos as Educational Tools

The use of video media, particularly YouTube videos, has emerged as highly popular and powerful educational tools in higher education due to its ability to provide rich and engaging content that enhances learners' understanding and retention of information throughout the learning process. Much of the current research highlights a need for more active learning strategies to maximize the educational potential of these videos because traditional Youtube videos tend to create passive learning experiences. However, when properly integrated with active learning strategies through innovative video design and development, Youtube videos can enhance student engagement, learning outcomes, and satisfaction. Therefore, this research aims to innovatively design and develop YouTube videos as educational tools and evaluate students' perceptions of their learning and the effectiveness of the video design and development in higher education involving 20 graduate students in the entrepreneurship education program. The data were analyzed using mean, standard deviation, and content analysis techniques. The findings showed that the innovatively developed Youtube videos were perceived to be highly appropriate (Mean= 4.52, S.D. = 0.13). Students reported that they learned entrepreneurship more effectively from various scenarios filled with choices and challenges in entrepreneurial paths. They also perceived the effectiveness of Youtube videos as educational tools to be very high (Mean= 4.58, S.D. = 0.72), noting that these videos were effective tools that promotes active and experiential learning experiences. In conclusion, this study indicates that Youtube videos, when innovatively designed and developed as educational tools with active learning strategies, can be implemented as a powerful tool in teaching entrepreneurship.

Profiling of Potential Anti-Diabetic Active Compounds in White Tea: An Integrated Study of Polyphenol-Targeted Metabolomics, Network Pharmacology, and Computer Simulation.

Diabetes remains a critical global public health challenge, posing a growing threat to human health and well-being. White tea is a lightly fermented tea and one of the six traditional tea categories in China. Owing to its rich content of bioactive compounds such as catechins and alkaloids, it has demonstrated potential anti-diabetic properties. However, its precise bioactive components, mechanisms of action, and relevant molecular targets require further investigation. In this study, an integrated approach combining polyphenol-targeted metabolomics, in vitro antioxidant assays, α-glucosidase inhibition tests, network pharmacology analysis, GEO database exploration, molecular docking, and molecular dynamics simulations was employed to identify the potential anti-diabetic compounds, targets, and mechanisms of white tea. The findings revealed that white tea is particularly abundant in 10 bioactive compounds, including epigallocatechin gallate, epicatechin gallate, and catechin, all of which exhibit significant anti-diabetic potential. These compounds were found to exert their effects by interacting with core molecular targets, namely cathepsin V (CTSV) and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), and engaging in pathways related to signal transduction, apoptosis, and immune responses. This study establishes a strong theoretical basis for advancing white tea research and underscores new opportunities for applying natural products in diabetes therapy.

Natural ways of vitamin D supplementation – detailed evaluation of Cantharellus cibarius

Introduction and purpose: In the past, fungi were considered a very low-value meal, however in modern literature, they are increasingly more frequently studied for their nutritional and medical properties. They are being currently reconsidered as an abundant source of nutrients that may be incorporated in diets of individuals of special medical needs, such as obese and diabetic patients. The purpose of this review is to evaluate one of the most common edible mushroom species, Cantharellus cibarius (girolle, golden chanterelle) for its health promoting properties with particular emphasis on its vitamin D content. It was of considerable importance for us to analyse the amount of vitamin D in the mentioned mushroom, as this micronutrient deficiency is fairly common in the human population. Material and method: The available literature was reviewed as provided by PubMed database and Google Scholar, with the use of keywords. State of knowledge: Golden chanterelle mushroom is a valuable source of proteins, mineral ingredients like potassium, phosphorus, magnesium, iron and calcium, while containing low amounts of sodium and fat. The mushroom is especially valuable for its rich content of vitamin D, ranging up to 63 μg/100g fresh weight. Conclusions: This paper may help other scientists expand their research on golden chanterelle properties. Moreover, girolle, as naturally containing many pro-health metabolites, may serve as natural supplementation of microelements.