Polished Rice Research Articles

A typical presentation of a laryngeal foreign body in an infant

Background: A 1-year-old boy presented with a one-day history of fever and cough, potentially linked to an episode of aspiration during homemade rice feeding. Following the incident, he refused to feed, displaying irritability. The detection of neutrophil leucocytosis and dehydration prompted IV antibiotics and fluid. Persistent symptoms led to a lateral neck x-ray, revealing a radiopaque material near the epiglottis. Fibre optic laryngoscopy identified a metallic wire lodged in the right arytenoid cartilage and posterior pharyngeal wall, possibly a fragment from a rice polishing machine. Extraction under general anaesthesia resulted in minimal mucosal injury, and the child quickly recovered postoperatively.Conclusion: This report serves as a reminder to consider foreign body ingestion in the differential diagnosis, particularly in cases with subtle or unrelated symptoms in the paediatric age group. The multidisciplinary approach emphasises prompt intervention to prevent life-threatening complications in paediatric patients. Furthermore, employing awake fibre-optic laryngoscopy in the context of a suspected respiratory tract infection to confirm the presence of a foreign body is considered a clinically safer approach than immediately resorting to examination under anaesthesia.

Investigating the effects of grain quality, processing and environmental conditions on aflatoxin contamination in rice

This study examined the impact of aflatoxin contamination on rice, a widely consumed staple food. Various forms and types of rice from three regions in Pakistan between 2019 and 2022 were investigated for their relationship with processing, infestation severity and physicochemical characteristics. Semi-polished rice displayed the highest aflatoxin levels employing significant variability, while parboiled rice was also found to be contaminated. Nearly 22% of polished rice samples and 3% of brown rice samples exceeded the EU limit. Non-basmati rice varieties, particularly IRRI-6 (47.3 μg/kg), showed twice as much contamination as basmati rice. The coastal Zone-IV exhibiting significantly higher aflatoxin levels (avg. 14.40 µg/kg). Environmental conditions played a significant role, with moisture in brown rice and protein in parboiled rice exhibiting positive and negative correlations (p < 0.01) with aflatoxin levels, respectively. Principal Component Analysis (PCA) indicated that semi-finished and non-basmati rice were more associated with non-compliant rice. The first principal component, explaining 57.8% of variance, was positively loaded with aflatoxin, moisture, and insect infestation, while negatively loaded with protein, amylose, ash, immature, chalky, and foreign matter. These findings are insightful for local and international stakeholders for mitigating aflatoxin contamination in rice.

A CASE OF FOOD-DEPENDENT EXERCISE-INDUCED ANAPHYLAXIS DUE TO POLISHED RICE

A 14-year-old girl presented to our hospital with food-dependent exercise-induced anaphylaxis (FDEIA), possibly caused by rice. Despite experiencing four previous episodes of suspected FDEIA, she did not seek medical attention at her own discretion. On the fifth occurrence of symptoms, the general practitioner suspected FDEIA and referred the patient to our hospital. The only common factor in all five episodes was the consumption of rice, leading to the examination of the patient under suspicion of FDEIA caused by rice. Skin prick test results were positive for bran and polished rice, and exercise after consumption of polished rice resulted in anaphylaxis. Therefore, we diagnosed FDEIA caused by polished rice. Immunoblotting confirmed the presence of immunoglobulin E reacting with 14-16kDa rice bran protein in the patient's serum. The immunoblot inhibition test suggested that the rice bran protein to which the patient's serum reacted was also present in polished rice and no wash rice. As the patient may experience FDEIA after ingestion of no wash rice or rice flour, she was advised to eliminate these from her diet, treating them similarly to brown rice or polished rice.

Combination of isotope dilution with liquid chromatography-inductively coupled plasma mass spectrometry for the simultaneous monitoring and evaluation of cadmium and inorganic arsenic in polished rice.

A new monitoring and evaluation technique for cadmium (Cd) and inorganic arsenic (i-As) in rice was developed, where the isotope dilution (ID) method was applied in combination with high performance liquid chromatography-inductively coupled plasma mass spectrometry (LC-ICP-MS), following heat-assisted extraction. Cadmium and i-As in a rice sample were extracted using the HNO3-H2O2 extractant, and an appropriate amount of enriched 111Cd was spiked into it. Cadmium and As species were measured simultaneously by LC-ICP-MS. The cadmium concentration was calculated from the isotope dilution analysis, and i-As was determined by the comparison (1 point calibration) method using additional 111Cd as an internal standard. The proposed method provides accurate and precise determination of Cd based on the isotope dilution analysis. Moreover, it can be applied to a rapid screening test to find contaminated rice samples, by means of monitoring the intensity ratios of natural 111Cd and i-As to the additional 111Cd. The developed technique was applied to analyzing multiple rice reference materials, and the measurement results of Cd and i-As agreed with the certified values within the uncertainty range. It is noted that the grain size of rice samples does not affect the extraction data, when the rice sample is crushed into powder less than 850 μm. The proposed method was very useful for the monitoring and evaluation of Cd and i-As in rice as a precise analytical method as well as a screening method.

Evaluación nutricional y uso del polvillo de arroz en la alimentación de cuyes (Cavia porcellus)

Two studies were carried out with the aim of determining the digestible energy and the digestibility coefficient of the dry matter and protein of the rice polish; and assess the effect of five levels of rice polish (Oryza sativa) to replace wheat bran in diets for growing guinea pigs (Cavia porcellus) on productive parameters. In Study I, they were used 12 types I male guinea pigs with an average live weight of 875 g, the digestibility coefficients and digestible energy of rice polish were determined by the indirect method, with the total collection technique. The digestibility coefficient of dry matter was 80.33%, protein 75.51%, energy 78.06% and the digestible energy of rice polish was 3.77 Mcal/kg DM. In Study II, 80 type I improved male guinea pigs were used, of 14+2 days of age and average weight of 441 g, to evaluate the effect of five levels of rice polish: 0%, 4.5%, 7.5%, 15% and 30% in replacement of wheat bran, in 49-day growth diets. As a result, it was observed that there is no significant difference for weight gain, feed intake, feed conversion and carcass yield by replacing wheat bran with rice polish. The inclusion of rice polish at levels greater than 7.5 % affects the productive response in guinea pigs.

Cadmium accumulation dynamics in the rice endosperm during grain filling revealed by autoradiography.

Cadmium (Cd) is one of the environmental pollutants contaminated in our food. Several previous reports showed that rice polishing cannot be efficient to reduce Cd content in white rice, implying the characteristic Cd distribution in rice grain. However, Cd distribution has not been fully elucidated so far. Herein, 109Cd radiotracer experiment was performed using the rice seedlings at various time points after flowering to obtain autoradiographs of the brown rice to visually understand the Cd transport and distribution during the grain-filling process. It was shown that 109Cd accumulated in the outermost area of the brown rice, and also in the middle part of the starchy endosperm, resulting in the appearance of the double circle distribution pattern, which was not observed in the autoradiographs of 65Zn. The inner circle of 109Cd located around the center of the endosperm was developed particularly at around 8 and 10 days after flowering. After this period, 109Cd started to deposit at the outer part of the endosperm, which was also found in the autoradiograph of 14C-sucrose. Considering the physiology of grain development, the contribution of water transport and protein synthesis in the endosperm on the characteristic Cd distribution pattern was hypothesized.

Effects of different traditional and commercial feed on growth, survival and proximate composition of Rohu (Labeo rohita) reared in the semi-intensive composite culture system.

This study was investigated to assess the effects of different traditional and commercial aqua feed on proximate composition, growth performance and survival rate of Labeo rohita reared in the composite semi-intensive culture system. The aqua feeds of various companies (AMG, Supreme, Aqua, Star Floating, Hi-Pro and Punjab feed) used as commercial feed. Farm made feeds were maize gluten and rice polish. For confidentiality, these feeds were randomly given code labels T1, T2, T3, T4, T5, T6, T7 and T8 which were only known to investigating staffs. There were two replicates for each treatment. In this experiment, higher growth rate was observed in T3 as compared to other treatments. Lesser weight gain was observed in the T1 ( 270.30 ± 0.5 ). The maximum body length ( 19.25 ± 2.19) was found in T3. Similarly, the minimum body length (5.97 ± 2.94) was seen in T2. FCR ratio (2.36 ± 0.01 ) was recorded in T3. Simultaneously, FCR (1.86 ± 0.002 ) was also recorded in T4 that is the perfect ratio for farmers. Higher SGR was noted in T3 (1.62 ± 0.05 ). Overall, T4 showed lesser SGR (1.05 ± 0.001 ). T4 showed the higher crude protein ( 28.66 ± 0.24 % ). In the body composition higher level of fat content was recorded in T3 ( 5.46 ± 0.33 %). These outcomes also proved that the rise in the dietary protein level and lipid content can improve the fish's body crude fats and protein level. Thus, based on growth performance, survival and proximate composition. It is concluded that T3 and T4 may be recommended for commercial culture of L. rohita.

Evaluation of Nutritional Composition of Small Ruminants’ Feed stuffs widely grown in Khulna Region of Bangladesh

Feed stuffs availability is one of the extreme obstacles to small ruminant production, particularly during the dry season. In Khulna region, there are several feed stuffs, fodders, grasses and weeds, but their potential has not yet been completely realized due to a lack of understanding of their nutritional value. Therefore, the research was carried out to determine the nutritional value of available feed stuffs for small ruminants in the Khulna region. Roughage and concentrate samples were collected from different areas of Khulna region. All of the samples were taken in triplicates and analyzed accordingly. The results revealed that among the grass species, the Gitlacontains the highestamount of DM content (39.74%)and lowest in Kalmilata (13.90%). The crude protein contents in grass species ranged from 6.5% to 25.65% being highest in Helencha and lowest in Para. Highest EE content was found in German lata (7.05%), followed by Kakpaya (6.72%). The CF content varied between 9.39% in Chanchito 40.05%in Para. Kakpayacontains the highest ash (17.45%), while Pakchong the lowest (8.68%).Mustard oil cake containsthe highestamount of DM (94.77%) among the concentrates, whereas red wheat contains the lowest (89.85%). Crude protein (CP) contents of concentrate feed stuffs ranged from 5.35% in gram bran to 50.75% in soybean meal. Rice polish (21.12%) showed the highest EE concentration and it was lowest in motor bran (2.50%). The maximum concentration of CF was found in Khesari bran (27.17%), while the lowest was in maize (1.82%). It could be concluded that evaluated concentrate feeds and grass species could serve as prospective livestock feeds in southwest Bangladesh. South Asian J. Agric., Vol. 9, No.1&amp;2, 2022-‘23: 31-38

Nutritional Assessment and Comparison between Brown and Milled Rice Landraces (Anadi, Bhotange, and Kalo Nuniya) of Nepal

The main objective of this study was to assess and compare the nutritional quality of brown- and milled rice landraces (Kalo Nuniya, Anadi, and Bhotange) of Koshi Province. Paddy samples were cleaned to remove dockage and sun-dried until about 13-14% moisture to ensure maximum hulling- and milling recovery. Paddy samples were shelled and milled to obtain brown (unpolished) rice and milled (white or polished) rice, respectively and were stored in double-sealed re-closable low-density polyethylene (LDPE) zipper bags (62.5 µm) at ambient temperature (23-27°C) until analysis. As expected, brown rices had better nutritional profiles than their milled counterparts with significantly higher (p&lt;0.05) crude fat (2.99± 0.10 − 3.99 ± 0.09%), crude fiber (0.12 ± 0.00 − 0.70 ± 0.02%), and total ash (1.53 ± 0.2 − 3.71 ± 0.30%) content. Among the brown rices, Anadi brown had better nutritional profile with the highest iron (1.56 ± 0.14 mg/ 100 g), calcium (86.4 ± 3.30 mg/100 g), total ash (3.71 ± 0.30%), crude fiber (0.70 ± 0.02%), and crude fat content (3.99 ± 0.09%). Bhotange and Kalo Nuniya had uniform distribution of iron and calcium, respectively, throughout the entire rice kernel.

基于离散元模拟的白米仿真参数标定

Aiming at the lack of discrete element simulation models and parameters for rice polishing, grading, color sorting and other technologies and equipment, and the difficulty of guiding equipment design and optimization through simulation, this paper calibrates the simulation parameters of white rice based on angle of repose (AOR) test and simulation methods. Huanghuazhan and Dongnong 429 white rice were selected as research object. Numerical model of white rice was established by multi-sphere filling. According to physical test and references, the simulation parameter range of white rice particles was determined. Plackett-Burman test was used to screen parameters, and it was found that the particle-particle static friction coefficient and particle-particle rolling friction coefficient had significant effects on the AOR of white rice. The regression model between the AOR and the significance parameter was established according to the central composite design method. The simulation parameter combination that has significant influence on the physical AOR was determined through the optimization design, and verified by the simulation test. The simulation AOR was compared with the physical AOR, and the relative error of the two kinds of white rice was less than 3%. The results show that the calibration method proposed in this study can accurately simulate the physical AOR test, which can provide reference for discrete element simulation of white rice processing.

Influence of Processing Stages in Cultivated Rice Varieties on the Feeding Preference of the Lesser Grain Borer, Rhyzopertha dominica F. (Coleoptera: Bostrichidae)

Rhyzopertha dominica (Coleoptera: Bostrichidae), a prevalent post-harvest pest in rice storage, significantly impacts global financial losses, necessitating a comprehensive understanding of its feeding habitats and processing phases. This study aimed to compare feeding preferences of R. dominica among four rice cultivars (Basmati 515, Super Basmati, Super Gold, and Super Basmati 2019) and three processing stages (paddy, brown rice, and polished rice). Rhyzopertha dominica exhibits distinct feeding preferences for rice cultivars and processing stages, with paddy of Super Basmati 2019 having the highest mortality rate (50.89%) and Basmati 515 polished rice having the highest survival rate (84.89%). The maximum growth rate (67.00 nos.), grain damage (80.67%), and grain weight loss (1.5806%) were seen on polished rice of Basmati 515. The study reveals that the processing stages of rice cultivars significantly influence R. dominica infestation and feeding preferences, with polished rice being the preferred stage. The study emphasizes the need for site-specific integrated pest management strategies to control R. dominica infestation in rice, highlighting the need for further research on its feeding preferences. To mitigate insect losses, improved management strategies should be implemented, including improved storage conditions, post-harvest handling practices, and a combination of physical and chemical control techniques.

Aloe barbadensis Effect on Growth Performance and Gastrointestinal Tract of Labeo rohita

Fish is a rich source of nutrients and help to reduce the inadequacy of calcium, vitamin A, iron, protein and cure many diseases. Labeo rohita (Rohu) is commercial species of Pakistan and main food item. The base of this study is to enhance the growth of Labeo rohita under special dietary condition. The aim of this research project was to check the effect of traditional herb Aloe barbadensis on Growth rate, FCR, Survival rate and Gastrointestinal villi histopathology of Labeo rohita fed on different Aloe barbadensis concentration containing diet.&#x0D; Methodology: Initial stocks of 120 Labeo rohita fingerlings having average weight 14.30 +3.51g were bought from Government Fish Hatchery, Faisalabad. Aloe barbadensis leaf’s powder were prepared ad mixed with different concentration of conventional feed ingredients like such as fish meal, rice polish, corn, wheat bran. Four treatments were designed including a control and Aloe barbadensis incorporated in the fish feed at 0%, 05% and 10%, 15% which were administrated for a period of 180 days. This feeding trial remains continued till 180 days in aquarium. Iso nitrogenous diets having protein level 33±1g.45 but different concentration of Aloe barbadensis were prepared and fed to fingerlings. Ingredients quantity was calculated by using Pearson square method. Conventional feed ingredients i.e. fish meal, rice polish, corn and wheat brane were used in feed in pellet forn. At the end of feeding trial three fish from each aquarium were selected randomly for gastrointestinal analysis. Gastrointestinal Villi parameters like length, width of intestine villi were measured at the end of trail.&#x0D; Results: According to results, different levels of Aloe barbadensis leave’s powder had significant positive effect upon Growth, Survival rate, and FCR. Results showed that Aloe barbadensis at 15% (T3) resulted in improved growth rate, Survival rate and feed conversion ratio (FCR). The Aloe barbadensis-treated groups showed improvement in intestinal villi length, width and gap between villi. The present study suggests that Aloe barbadensis especially at 15% feed administration may enhance effectively the growth performance and gastrointestinal (villi) parameters like length, width and gap between villi of Labeo rohita.

Effects of Different Aquafeed Sources on Growth Performance, Oxidative Capacity, and Fatty Acid Profile of Three Carps Reared in the Semi-Intensive Composite Culture System.

The current experiment is designed to evaluate the effect of different aquafeeds (farm-made versus commercial) on growth, body composition, oxidative capacity, and fatty acid profile in the semi-intensive composite culture system. For this, 1,100 fingerlings/acre having initial body weight and length, Labeo rohita (61.34 g, 171 mm), Catla catla (71.45 g, 181 mm), and Cyprinus carpio (30.80 g, 91 mm) were randomly distributed to 16 ponds and randomly fed on eight different diets (n = 2 pond/diet) in a completely randomized research design. Aquafeed were farm-based diets (D1-D2) and commercial aquafeed (D3-D8). The farm-made diets contained various crude protein levels of maize gluten (24.9%) and rice polish (7.3%), whereas commercial diets were procured from commercial feed plants (AMG, Supreme, Aqua, Star Floating, Hi-Pro, and Punjab feed). The growth performance of carps (L. rohita and C. catla) was significantly improved (p < 0.05) by feeding D3 as compared to other diets. Similarly, white blood cell concentration was greater (p < 0.05) in all species fed by D3 than in those fed on D7, D8, D5, D6, D1, and D2 fed groups, respectively. Alanine transaminase, aspartate transaminase, and alanine phosphatase activities were significantly lower (p < 0.05) in the D3-fed L. rohita, C. catla, and C. carpio compared with those fed on the rest of the treatments. The activities of glutathione peroxidase and superoxide dismutase were also higher (p < 0.05) for the D3 fed L. rohita, C. catla, and C. carpio than those fed on the rest diets. The groups fed on D3 and D4 had greater (p < 0.05) concentrations of myristic (14), palmitic acid (16), and stearic (18) acids than those fed on the rest of the commercial diets. However, meat chemical composition was similar (p > 0.05) across the treatments. These results also prove that the increase in the dietary protein level and lipid content can improve the fish's body's crude protein and fat levels. Feeding D3 improved the production performance, oxidative status, and fatty acid profile in composite major carps culture systems. Thus, based on growth, survival, and body composition, it is concluded that D3 and D4 may be recommended for a commercial culture of major carps. Dietary treatments had no significant impact (p > 0.05) on water's physical-chemical properties. Calcium content and alkalinity varied (p < 0.05), with D5 showing the lowest calcium and the highest alkalinity.

Bioaccessibility assessment of arsenic and cadmium in polished and unpolished rice: Comparison of three in vitro methods

INFOGEST is a standardized in vitro digestion method suitable for foods, but rarely used to study the bioaccessibility of heavy metals in food. This study aimed to explore the differences between INFOGEST and the extensively used Physiologically Based Extraction Test (PBET) and Unified Bioaccessibility Research Group of Europe Method (UBM) methods for determining the bioaccessibility of As and Cd in rice. Intestinal As (79.3 ± 8.5 %, 75.8 ± 12.7 %, and 72.3 ± 12.2 % for INFOGEST, PBET, and UBM, respectively) and Cd (47.0 ± 6.4 %, 40.7 ± 13.8 %, and 38.1 ± 15.7 % for INFOGEST, PBET, and UBM, respectively) bioaccessibilities in the rice samples determined by the three methods were generally similar (p > 0.1, except for As bioaccessibility between INFOGEST and UBM). Furthermore, PBET was significantly correlated with INFOGEST for As bioaccessibility (R2 = 0.416) and with UBM for Cd bioaccessibility (R2 = 0.879). Additionally, PBET indicated that the bioaccessibilities of As and Cd in the polished rice were 17.0 % and 19.8 % higher, respectively, than that in the unpolished rice. This study highlights the influence of in vitro methods and rice matrices on heavy metal bioaccessibility values, necessitating a more accurate assessment of health risks associated with rice consumption.

Occurrence of Pesticides in Polished Rice Samples From Tanjung Karang and Sekinchan and the Health Risk among Consumers

Introduction: One of the key staple foods in many different countries, including Malaysia, is rice (Oryzae sativa L.). Pesticides are used to reduce weed growth and safeguard crops from insect attacks in order to boost paddy output. Objective: This research quantifies the concentration of pymetrozine, chlorantraniliprole, and difenoconazole, along with any potential health risks to consumers. Methods: Samples of polished rice from three rice milling factories in Tanjung Karang and Sekinchan were extracted using the QuEChERS (Quick Easy Cheap Effective Rugged Safe) technique. The amount of pesticide residue in polished rice was then measured using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). A health risk assessment was conducted utilizing the Hazard Quotient (HQ) and Lifetime Cancer Risk (LCR) to estimate the non-carcinogenic and carcinogenic health risks. Results: The mean concentration of pesticides in polished rice ranged from less than the detection limit (difenoconazole) to 1.122 µg/kg (pymetrozine). No rice samples above the Maximum Residue Limit (MRL) specified in the Food Act 1983 as implemented by Malaysia’s Food Regulations 1985. Pymetrozine, chlorantraniliprole, and difenoconazole in rice had respective MRLs of 50 µg/kg, 2000 µg/kg, and 100 µg/kg. For all age groups, no significant non-carcinogenic health risk was associated with consuming polished rice from the study area, where the HQ and Hazard Index (HI) were less than one. When LCR values were less than 10-6, carcinogenic health risks of consuming polished rice from the study area were at a clearly acceptable risk level. Conclusion: As no sample exceeded the MRL, the findings indicated that all rice samples collected from Tanjung Karang and Sekinchan were safe for consumption.

OsDWARF10, transcriptionally repressed by OsSPL3, regulates the nutritional metabolism of polished rice.

Strigolactone (SL) plays essential roles in plant development and the metabolism of rice leaves. However, the impact of SL on the accumulation of nutritional metabolites in polished rice, as well as the transcription factors directly involved in SL synthesis, remains elusive. In this study, we performed a metabolome analysis on polished rice samples from mutants of an SL biosynthetic gene, OsDWARF10 (OsD10). Compared with those in the wild type plants, primary and secondary metabolites exhibited a series of alterations in the d10 mutants. Notably, the d10 mutants showed a substantial increase in the amino acids and vitamins content. Through a yeast one-hybridization screening assay, we identified OsSPL3 as a transcription factor that binds to the OsD10 promoter, thereby inhibiting OsD10 transcription in vivo and in vitro. Furthermore, we conducted a metabolic profiling analysis in polished rice from plants that overexpressed OsSPL3 and observed enhanced levels of amino acids and vitamins. This study identified a novel transcriptional repressor of the SL biosynthetic gene and elucidated the regulatory roles of OsSPL3 and OsD10 on the accumulation of nutritional metabolites in polished rice.

Genome-Wide Association Study of Arsenic Accumulation in Polished Rice.

The accumulation of arsenic (As) in rice poses a significant threat to food safety and human health. Breeding rice varieties with low As accumulation is an effective strategy for mitigating the health risks associated with arsenic-contaminated rice. However, the genetic mechanisms underlying As accumulation in rice grains remain incompletely understood. We evaluated the As accumulation capacity of 313 diverse rice accessions grown in As-contaminated soils with varying As concentrations. Six rice lines with low As accumulation were identified. Additionally, a genome-wide association studies (GWAS) analysis identified 5 QTLs significantly associated with As accumulation, with qAs4 being detected in both of the experimental years. Expression analysis demonstrated that the expression of LOC_Os04g50680, which encodes an MYB transcription factor, was up-regulated in the low-As-accumulation accessions compared to the high-As-accumulation accessions after As treatment. Therefore, LOC_Os04g50680 was selected as a candidate gene for qAs4. These findings provide insights for exploiting new functional genes associated with As accumulation and facilitating the development of low-As-accumulation rice varieties through marker-assisted breeding.

Panicle Architecture in Enhancing Grain Yield in Rice (Oryza sativa L.)

The architecture of the rice panicle is a crucial focus in breeding for both high yield and quality. It stands as a significant agronomic trait that influences the number of grains per panicle, playing a direct role in contributing to the overall yield of rice grains. Unravelling the genetic basis of panicle architecture is crucial for improving the grain yield in rice. In this study, the panicle architecture traits were meticulously dissected into five distinct components viz., number of primary rachis, number of secondary rachis, grain number on primary rachis, grain number on secondary rachis and total grain number. These components were systematically phenotyped in F2 and F3 population derived from a cross between DRR Dhan 48 and Maudamani. ‘DRR Dhan 48’ is a biofortified elite fine grain medium slender grain type cultivar with high zinc (22 ppm in polished rice) and low glycemic index (51.1). It has resistance to bacterial blight with the incorporation of xa5, xa13 and Xa21 in the background of Improved Samba Mahsuri. ‘Maudamani’ is a high yielding cultivar with high grain number and short bold grain type. Analysis of variance, histograms and boxplots revealed highly significant variation for the studied traits. Grain number in F2 population ranged from 29 to 333 grain with a mean of 162.72 whereas in F3, its varied from 49 to 368 with a mean of 184.17. Correlation analysis revealed significant correlation among the studied traits. The continuous variation observed in the population for panicle traits indicates that genetic control is governed by multiple minor loci. The presence of superior transgressive segregants highlights a complementary gene action influencing panicle architecture traits. The present investigation on panicle architecture offers scope for improving rice varieties and creating new germplasm resources and provides valuable information for further unravelling the genetic basis determining rice panicle architecture.

Rice Nutraceuticals and Bioactive Compounds: Extraction, Characterization and Antioxidant Activity: A Review.

Rice (Oryza sativa L.), a cereal grass, belongs to the genus Oryza from the family Poaceae, which encompasses twenty-five species cultured in many countries of Asia, and partly in the rest of the world. From these species, two viz. Oryza sativa (O. sativa) Asian rice and Oryza glaberrima (O. glaberrima) African rice are commonly found and the most widely consumed staple food by a large part of the human population in the world, especially in Asia due to their nutritional and nutraceutical prospects. Rice, a popular source of carbohydrates, also contains a good amount of dietary fiber, minerals (Ca, Zn, Se, P, K, Mg, Fe, and Mn), protein and vitamin B along with several other medicinally important bioactives such as tocols (α-tocopherols and α-tocotrienols) (ßsitosterol) phenolic acids, flavonoids (apiginine), and oryzanol (24-Methylenecylcoartanyl transferulate). Rice bran is a byproduct of the rice polishing industry and is valuable in terms of containing 15-20% high-value oil. Because of the natural antioxidants present in rice, several medicinal benefits and biological properties can be attributed to rice consumption. The nutrient profile of rice varies based on several factors, such as grains (white, brown, red, and black/purple), the extent of polishing, and the preparation method. Considering the importance of rice as a traditional diet rich in high-value bioactives, together with the existing gap of related information, it is worthwhile to assemble a comprehensive review that focuses on the detailed profile of valuable nutrients and high-value phytochemicals and biological activities of rice to explore its functional food and nutraceutical applications. This review attempts to provide collective information on the essential rice cereal for its nutritional and antioxidant potential.

Characterization of heterogeneity in popular rice landrace through field and molecular evaluation

ContextIdentification and purification of the landrace Chittimuthyalu using stability and molecular heterozygosity can be useful for rice diversity. ObjectivesThe present study reports the characterization of 12 genotypic variants of Chittimuthyalu in terms of (i) variation and correlation among agromorphological, grain quality and nutrient traits, viz., Zn and Fe; (ii) stability across years; and (iii) characterization and heterozygosity. MethodsA total of twelve genotypic variants were identified based on field-level agromorphological observations in the Chittimuthyalu landrace. For identification of a stable genotypic variant for grain Zn, genotype by environment (G × E) interaction was analyzed across three years using additive main effects and multiplicative interaction (AMMI) model and genotype main effects plus G × E (GGE) model for multienvironment data analysis along with principal component analysis (PCA). Correspondingly, twenty-five high polymorphism information content (PIC) RM markers were used for the identification of molecular heterozygosity in landrace. ResultsStepwise regression analysis of phenotype revealed the interdependence of zinc in polished rice (ZPR), iron in polished rice (IPR), and single plant yield (SPY) and the positive association of grain Zn with IPR and SPY. Based on GGE and AMMI analyses of the variants, MCM3 was found to be stable across the environments for ZPR. Out of 46% polymorphic RM markers, seven markers (RM10149, RM13347, RM21749, RM13263, RM10361, RM12276 and RM474) were heterozygous. Heterozygous alleles were observed in eight variants (MCM2, MCM3, MCM5, MCM6 MCM7, MCM8, MCM10 and MCM12). The polymorphic information content (PIC) of the markers ranged from 0.58 to 0.88, with an average of 0.64. The percentage of the most common allele frequencies ranged from 4.2 to 95.8 across the markers. RM13347 showed nine markertrait associations with P ≤ 0.05 and R2 ranging from 2.3% to 14.3% with a mean of 7.74%. ConclusionsThe present study has revealed the inherent heterogeneity in landraces in terms of their agromorphological, yield-related and nutritional quality traits. A promising genetic stock (MCM3) was identified as a donor for high grain ZPR and was registered as genetic stock. ImplicationsThe identified genetic stock is being shared with breeders in the breeding programme and farmers for the multiplication of high grain Zn rice.