This study aimed to examine the effectiveness of acetoin-producing rhizobacteria formula in promoting growth and increasing local rice yields in Merauke. This in vitro ponder was conducted at the Biopesticide Research facility, Staff of Horticulture, Udayana College. This think about was carried out within the test cultivate of the Staff of Agribusiness, Udayana College, Denpasar. This think about focused on four rhizobacteria segregates that have been recognized to be tried to fortify the development of rice plants. The rice assortment utilized was the Ciherang assortment, which was frequently developed by cultivating communities in Merauke Rule. The test plan utilized was a Randomized Piece Plan (RBD), comprising of 4 replications. Each replication comprised of 6 medications, to be specific 4 rhizobacteria separates and 2 medicines for comparison. The four rhizobacteria confines were Rg21, Pd13, Pd7, and Bb7. Whereas the 2 medicines as a comparison were Unadulterated Acetoin (Dad ) and control. Each treatment was rehashed 10 times so that the whole reiteration was 240 rice plant pots. The pot measure utilized was a surface with a distance across of 30 cm and was filled with developing media. The results of the study showed that the mechanism of action of the rhizobacterial formula in increasing rice yields was the presence of acetoin compounds and derivative compounds such as 2-Butanone, 3-hydroxy, 2,3-butanediol, diacetate, 2,3-Butanediol (CAS), 2-Butanone, 3-acetyloxy, and 1,4-Dioxane. These compounds were produced by rhizobacteria in the rhizosphere, which helped plants achieve acetoin homeostatic conditions so that plants could increase plant height, leaf area, number, number of productive tillers, panicle length, percentage of filled and empty grain, and reduce amylose content. The treatment of Merauke local rhizobacteria formula carried out in a greenhouse with a concentration of 2% was effective in promoting growth and increasing rice yields by adding the weight per plant by 52.83% when compared to the control.