Growth Of Rice Plants Research Articles

Effect of Planting Dates, Potassium Fertilizer Resources and Varieties on Growth, Yield and Proline of Rice Plants Under Rainfed Lowlands

Effect of Planting Dates, Potassium Fertilizer Resources and Varieties on Growth, Yield and Proline of Rice Plants Under Rainfed Lowlands

Silicon and iron nanoparticles protect rice against lead (Pb) stress by improving oxidative tolerance and minimizing Pb uptake

Lead (Pb) is toxic to the development and growth of rice plants. Nanoparticles (NPs) have been considered one of the efficient remediation techniques to mitigate Pb stress in plants. Therefore, a study was carried out to examine the underlying mechanism of iron (Fe) and silicon (Si) nanoparticle-induced Pb toxicity alleviation in rice seedlings. Si–NPs (2.5 mM) and Fe-NPs (25 mg L−1) were applied alone and in combination to rice plants grown without (control; no Pb stress) and with (100 µM) Pb concentration. Our results revealed that Pb toxicity severely affected all rice growth-related traits, such as inhibited root fresh weight (42%), shoot length (24%), and chlorophyll b contents (26%). Moreover, a substantial amount of Pb was translocated to the above-ground parts of plants, which caused a disturbance in the antioxidative enzyme activities. However, the synergetic use of Fe- and Si–NPs reduced the Pb contents in the upper part of plants by 27%. It reduced the lethal impact of Pb on roots and shoots growth parameters by increasing shoot length (40%), shoot fresh weight (48%), and roots fresh weight (31%). Both Si and Fe–NPs synergistic application significantly elevated superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and glutathione (GSH) concentrations by 114%, 186%, 135%, and 151%, respectively, compared to plants subjected to Pb stress alone. The toxicity of Pb resulted in several cellular abnormalities and altered the expression levels of metal transporters and antioxidant genes. We conclude that the synergistic application of Si and Fe-NPs can be deemed favorable, environmentally promising, and cost-effective for reducing Pb deadliness in rice crops and reclaiming Pb-polluted soils.

Regulation of growth and stress metabolism in rice plants through foliar and root application of seaweed extract from Kappaphycus alvarezii (Rhodophyta)

Regulation of growth and stress metabolism in rice plants through foliar and root application of seaweed extract from Kappaphycus alvarezii (Rhodophyta)

Vegetative Growth Response of Rice in Heavy Metal Contaminated Soil Due to NPK Fertilizer Based Ameliorant Application

Heavy metal pollution in paddy fields has been recognized as a global environmental problem that must be addressed. Exposure to heavy metals causes stunted growth of rice plants. A safe and environmentally friendly approach is done through the use of a combination of ameliorants and inorganic fertilizers. NPK Co-Ameliorant fertilizer is an innovation in bio-agent multifunctional fertilizer production technology that is expected to be an alternative solution to the widespread pollution of rice fields by industrial waste. This study aims to examine the effect of NPK Co-Ameliorant fertilizer on vegetative growth of rice plants on industrial waste polluted soil. The research was conducted in a greenhouse with soil media from Jelegong village, Rancaekek which was polluted with heavy metals. The research period was from September 2023 to February 2024. The experimental design used a Randomized Group Design with eight treatments, four replications and two units consisting of: 0 (control); NPK Phonska 225 kg ha-1; and various doses of NPK Bio-Organomineral fertilizer 150; 250; 350; 450; 550; 650 kg ha-1. The results showed that the application of NPK Co-Ameliorant fertilizer effectively increased rice plant height, number of tillers, chlorophyll content and plant biomass weight. The dose of NPK Co-Ameliorant fertilizer 350 kg ha-1 was the best dose in increasing the height of rice plants reaching 95,63 at 70 DAP, increasing the chlorophyll content of plants by 24.20 CCI and increasing the dry weight of plants reaching 33,98 g.

MiR408 balances plant growth and heat response in rice

MicroRNAs are small noncoding RNAs that regulate gene expression by targeting complementary mRNA molecules. Among them, microRNA408 (miR408) is an ancient microRNA influencing plant growth and aiding in abiotic stress adaptation. We proved that miR408 sliced uclacyanin7 (UCL7) directly by degradome sequencing. However, the mechanisms underlying the balance between growth and stress response of rice (Oryza sativa L.) controlled by miR408 remain unclear. We conducted a study comparing Nipponbare (wild-type, WT) plants with miR408–3p-overexpression (miR408–3p-OX) and UCL7-overexpression (UCL7-OX) plants under normal and heat stress conditions. Through RNA-sequencing, physiological measurements, and phenotype examination, we observed that miR408 overexpression resulted in increased dry weight and grain yield, higher levels of ATP and non-structural carbohydrates (NSC), improved photosynthesis, and reduced H2O2 content, antioxidant enzyme activity, and ATPase activity in seedling plants compared to UCL7-OX plants. Additionally, the application of H2O2 scavengers, such as ascorbic acid (ASA) and dimethylthiourea (DMTU), further enhanced dry weight and photosynthesis by reducing H2O2 levels. Under heat stress conditions, miR408 overexpression sharply increased H2O2 content, antioxidant enzyme activity, ATP levels, and ATPase activity compared to UCL7-OX plants, thereby enhancing heat tolerance in rice seedlings. These findings suggest that miR408 and its target gene UCL7 participate in regulating energy homeostasis to achieve a balance between growth and heat response. This regulation involves modulating H2O2 levels and optimizing the relationship between the target of rapamycin (TOR) and SNF1-related protein kinase 1 (SnRK1) in rice.

The role of endophytic bacteria in increasing nitrogen concentration, root length, and dry weight of rice plants (Oryza sativa L.) in saline soil

Soil salinity can interfere with the absorption of water and plant nutrients, causing a decrease in growth and yield of rice plants in saline soil. This negative effect can be reduced by the application of endophytic bacteria biofertilizer. Endophytic bacteria live in plant tissues and play a role in increasing plant growth through nitrogen fixation or growth hormone production. The purpose of this study was to determine the effect of endophytic bacterial biofertilizer application on N concentration, root length, and dry weight of rice seedlings (Oryza sativa L.) in soils with different salinities. This research was conducted in the greenhouse of the Faculty of Agriculture, Universitas Padjadjaran. The experimental design used was a Randomized Block Design (RBD) with 8 combinations. The treatments were with and without endophytic bacterial biofertilizers, and soils with different levels of salinity (non-saline soil and saline soil with salinities of 2, 4, and 6 mmhos cm-1) and repeated 4 times. The experimental results showed that the combination treatment of endophytic bacterial biofertilizer in saline soil of 2 mmhos cm-1 was able to increase the N concentration, root length, and dry weight of rice plants which were higher than the treatment of endophytic bacterial biofertilizer in soil with salinity of 4 and 6 mmhos cm-1. The application of endophytic bacteria biofertilizer has higher dry weight of rice plants than without endophytic bacteria at each different salinity level.

Plant Physiological Performances, Plant Growth, Grain Yield and Methane Emission of Rice (Oryza Sativa L.) in Response to Water Management as Adaptation Strategy for Climate Change

Continuously flooded rice systems are a major contributor to the greenhouse gases (GHG) emissions in the agriculture sector in Malaysia. Intermittent irrigation has been recommended to replace conventional rice water management to save water and reduce GHG emissions without compromising rice yields. This study was conducted in two growing seasons at Malaysia’s largest rice granary area to determine the effectiveness of different water management practices on conserving water, mitigating GHG and maintaining rice grain yields. Three water management treatments were continuous flooding (CF), saturated and wet conditions from transplanting to heading and flooding until maturity (S-F) and continuous saturated and wet conditions (CS). The results showed that S-F and CS reduced water inputs between 15.0-16.8% and 32.0-34.0% as compared to CF, respectively. Water-saving treatments mostly did not significantly affect the plant's physiological performance, plant growth parameters, growth rate, grain yield and yield parameters. The results indicated that soil saturated and wet conditions provided adequate soil moisture content for the plant’s requirement similar to flooding conditions. Maintaining soil at saturated and wet conditions during the vegetative stage reduced 24.18-39.76% of methane emissions. However, maintaining soil at saturated and wet conditions throughout the growing season reduced 34.52-55.08% of methane emissions. In conclusion, intermittent irrigation could be an effective adaptation technique for simultaneously saving water and mitigating GHG while maintaining high rice grain yields in rice cultivation systems.

Mining of rice rhizobacteria to control false smut and improve plant growth in rice (Oryza sativa L.)

Mining of rice rhizobacteria to control false smut and improve plant growth in rice (Oryza sativa L.)

Pembelajaran Aplikasi Pengukur Kandungan Klorofil pada Daun Tanaman Padi: Oryza sativa L.

Rice is a staple food in Indonesia, so the demand continues to increase along with the population growth. Rice cultivation in Indonesia is known for two planting systems, namely wetland rice which requires water and upland rice which is planted in fields without requiring special irrigation. Several regions in Indonesia have almost extinct upland rice varieties, such as the Fakhaito variety or black rice from Nias Regency, with a plant age of up to six months until harvest. Upland rice productivity is very low, affecting farmers' income, accompanied by suboptimal fertilization technology. This research aims to provide learning to students in using the SPAD-502Plus chlorophyll meter to detect the growth and health of upland rice plants from Nias. The results of the study show that treatment with liquid organic fertilizer correlates positively with leaf color, where the amount of chlorophyll is higher in the very green leaves of rice, and conversely the amount of chlorophyll decreases in plants with reduced greenness. The use of the SPAD-502Plus chlorophyll meter is a faster, non-destructive, comfortable, and non-disruptive way to measure plant growth, as the measurement is done directly.

Effect of Natural IAA Produced by Streptomyces Strain ACM37 on Seed Germination and Seedling Growth


 
 
 Biofertilizers play an important role in sustainable agriculture by improving soil fertility, crop tolerance and crop productivity. In this context, exploitation of plant growth promoting rhizobacteria with various beneficial properties is challenging. This study aimed to investigate the plant growth promoting activity of indole-3-acetic acid (IAA)-producing actinomycetes strain, Streptomyces ACM37 on the seed germination and seedling growth at early stage. The ACM37 was grown in starch-casein broth for 7 days at 28° C, 80 rpm and supplemented with 50 mg/l L- tryptophan to induce IAA production. After 7 days, IAA concentration in cell-free culture broth was quantified by following the Salkowski's method and it was 50 mg/l. Seed germination and seedling growth of cowpea (Vigna unguiculata) and rice (Oryza sativa L.) at different concentrations of IAA were estimated as % seed germination, number of roots, primary root length and length of hypocotyl. Initially, seeds were surface sterilized with 10% (v/v) NaOCl and soaked in 10, 20, 30, 40 and 50 mg/l crude IAA preparations for 1 h at 30° C. As controls, seeds were soaked in sterilized distilled water (SDW) and 50 mg/l synthetic IAA. Ten seeds per each treatment with three replicates were placed on 0.8% (w/v) water-agar in Petri plates and incubated in dark at 30° C. The % seed germination of ACM37-IAA-treated cowpea and rice seeds was enhanced at all concentrations compared to SDW and synthetic IAA treatments after 3 days. The highest 100% germination of cowpea was given at 10 mg/l IAA while it was 96.67±3.33% in>30 mg/l IAA in rice. Similarly, there was a significant (P<0.05) increase in primary root length and number of lateral roots in cowpea at 10 mg/l of ACM37-IAA. Overall, ACM37 showed significant plant growth promotion in cowpea at low concentration (10 mg/l) of IAA whereas there was a significant (P<0.05) reduction at high concentrations (>10 mg/l). On the other hand, there was no apparent increase in all growth parameters except seed germination in rice at all concentrations. It appears that high concentrations of ACM37-IAA may be required to promote plant growth in rice. Overall results highlight that plant growth promotion activity of ACM37 varies at different IAA concentrations and different plant species, probably between monocot and dicot species. Therefore, ACM37 has potential application as a plant growth promoting agent in biofertilizers and plant growth inhibiting agent in bioherbicides at certain IAA concentrations.
 Keywords: Actinomycetes, Biofertilizer, Bioherbicide, IAA
 
 

Growth performance, yield, and proline content of two local cultivars of black rice irradiated by gamma rays for drought resistance

One of the critical effects of climate change on the agricultural environment is drought stress. This study aimed to determine the growth, yield performance, and proline content of black rice local cultivars Bantul, Boyolali mutant M2 at various doses of gamma rays and the proline content of mutant M3 during drought stress. The experiment used a Completely Randomized Design (CRD) with the factor dose of gamma-ray irradiation (0 Gy, 100 Gy, 200 Gy, and 300 Gy), applied in both paddy fields and polybags to observe and compare the growth, yield, and proline content of black rice plants with the control treatment (0 Gy). Laboratory test results showed that Boyolali and Bantul cultivars irradiated at 100 Gy and 200 Gy, had the highest proline content, which increased under drought stress conditions in both cultivars. At 100 Gy, the average plant height and the number of productive tillers of black rice of Boyolali and Bantul cultivars were the highest, and the diversity of the two local cultivars at this dose was higher than at other irradiation doses. Applying 100 Gy and 200 Gy gamma-ray irradiation doses on the Boyolali cultivar significantly affected the panicle length character and the number of seeds per panicle. For the Bantul cultivar, panicle length and the number of seeds per panicle at an irradiation dose of 200 Gy showed significantly different results. The conclusion is that a reduced amount of gamma-ray irradiation can enhance the growth, yield, and proline content of M2 black rice

Utilization of solid organic waste as a biofertilizer formulation for upland rice plants supports sustainable rice farming

The use of local isolates as biofertilizer and organic fertilizer based on organic waste is carried out as an environmentally friendly cultivation effort towards sustainable agriculture. This research aims to obtain a biofertilizer formulation made from solid organic waste to support sustainable upland rice farming. The treatments used were: F0: without biofertilizer formulation, F1: 75% tofu dregs + 100 ml cellulolytic bacterial consortium, F2: 75% rice straw + 100 ml cellulolytic bacterial consortium, F3: 75% solids + 100 ml cellulolytic bacterial consortium, F4: 75% peat + 100 ml cellulolytic bacterial consortium, F5: 75% rice husk + 100 ml cellulolytic bacterial consortium. The formulation of organic waste-based biofertilizer with a consortium in this research showed that did not have an effect on the growth of upland rice plants, but the provision of 75% tofu dregs + 100 ml cellulolytic bacteria consortium tended to provide better plant height growth in terms of rice plant height parameters.

Iron reprogrammes the root system architecture by regulating OsWRKY71 in arsenic-stressed rice (Oryza sativa L.).

Iron (Fe) has been critically reported to act as a signal that can be interpreted to activate the molecular mechanisms involved in root developmental processes. Arsenic (As) is a well-known metalloid that restricts the growth and productivity of rice plants by altering their root architecture. Since root system architecture (RSA) under As stress targets WRKY transcription factors (TFs) and their interaction partners, the current investigation was carried out to better understand the Fe-dependent dynamics of RSA and its participation in this process. Here, we analyzed the effects of As and Fe (alone or in combination) exposed to hydroponically grown rice roots of 12-day-old plants. Our research showed that adding As to Fe changed how OsWRKY71 was expressed and improved the morphology and anatomy of the rice roots in Ratna and Lalat varieties. As + Fe treatment also manifested the biochemical parameters. OsWRKY71, revealed an up-regulation (Fe alone and As + Fe conditions) and down-regulation (As stress) in both varieties, in comparison to the controls. The improved root anatomy and root oxidizability indicated the enhanced capability of Lalat over the Ratna variety to induce OsWRKY71 for the better development of RSA during As + Fe treatment. Further, OsWRKY71 has revealed the presence of gibberellin-responsive cis-regulatory elements (GAREs) in its promoter region, indicating the involvement of OsWRKY71 in the gibberellin pathway. Molecular docking revealed that OsWRKY71 and SLR1 (DELLA protein) interact positively, which supports the hypothesis that Fe alters RSA by regulating OsWRKY71 through the gibberellin pathway in As-stressed rice.

Classification of Rice Growth Phases Using the K-Nearest Neighbor Algorithm in the Irrigation area of Seulimeum Sub District, Aceh, Indonesia

Rice (Oryza sativa L.) is the main food commodity for most of Indonesia’s population. The existence of irrigation areas dramatically affects the water demand in each phase of rice growth. Due to a lack of remote sensing knowledge, classifying the rice growth phase is done manually. This study aims to classify rice growth phases using the K-Nearest Neighbor Algorithm in the irrigation area of the Seulimuem Sub-District. This research uses Google Earth satellite imagery in November 2021 through three stages: multi-resolution segmentation, guided classification, and accuracy testing. The results showed that the paddy and non-paddy fields are 1,425.56 ha and 6,581.70 ha, respectively. The classification results of rice plant growth phases consist of 4 phases, namely the inundation phase, the vegetative phase, the generative phase, and the fallow phase. Based on the accuracy assessment results in November 2021, the overall accuracy ranged from 90.65% to 92.92%, with a kappa index value of 0.85 to 0.87, categorizing it as “almost perfect.”

Efektivitas Bakteri Endofit Asal Lahan Basah untuk Menekan Penyakit Hawar Daun Bakteri (Xanthomonas oryzae) pada Padi Beras Merah (Oryza nivara L.)

Brown rice is a type of rice that has many benefits for the health of the body. The main diseases that attack many rice plants and cause a decrease in production include bacterial leaf blight (HDB) caused by the bacterium Xanthomonas oryzae. One alternative to control HDB is to use antagonistic agents, namely endophytic bacteria. This study aims to examine the ability of endophytic bacterial isolates from wetlands and the effect of application time in suppressing the intensity of bacterial leaf blight and to determine its effect on the growth of brown rice plants. There were 2 isolates of endophytic bacteria tested in this study, namely isolates of root origin (AKL) and isolates of stem origin (BLR) isolated from karamunting plants. This study used a completely randomized design (CRD) consisting of 5 treatments and 4 replications. The treatment used in this study was only the pathogen Xanthomonas Oryzae (control), soaking seeds with endophytic bacteria from roots, soaking seeds with endophytic bacteria from stems, soaking seeds + application when transplanting with endophytic bacteria from roots and seed soaking + application when transplanting Plant with stem-origin endophytic bacteria. Treatment of endophytic bacteria on brown rice seeds and application during transplanting was able to reduce the intensity of bacterial leaf blight (X. oryzae) with a disease intensity of 83% and an effectiveness of 15.6%. Treatment of endophytic bacteria on rice seeds did not inhibit seed germination and was able to increase the number of tillers, but had not been able to affect the increase in plant height.

Multifaceted response mechanisms of Oryza sativa L. 'KDML105' to high arsenite and arsenate stress levels.

Toxicity resulting from high levels of inorganic arsenic (iAs), specifically arsenite (AsIII) and arsenate (AsV), significantly induces oxidative stress and inhibits the growth of rice plants in various ways. Despite its economic importance and significance as a potent elite trait donor in rice breeding programmes, Khao Dawk Mali 105 (KDML105) has received limited attention regarding its responses to As stress. Therefore, this study aimed to comprehensively investigate how KDML105 responds to elevated AsIII and AsV stress levels. In this study, the growth, physiology, biochemical attributes and levels of As stress-associated transcripts were analysed in 45-day-old rice plants after exposing them to media containing 0, 75, 150, 300 and 600µM AsIII or AsV for 1 and 7days, respectively. The results revealed that AsIII had a more pronounced impact on the growth and physiological responses of KDML105 compared to AsV at equivalent concentrations. Under elevated AsIII treatment, there was a reduction in growth and photosynthetic efficiency, accompanied by increased levels of hydrogen peroxide (H2O2) and malondialdehyde (MDA). Notably, the total contents of antioxidants, such as proline, phenolics and flavonoids in the shoot, increased by 8.1-fold, 1.4-fold and 1.6-fold, respectively. Additionally, the expression of the OsABCC1 gene in the roots increased by 9.5-fold after exposure to 150µM AsIII for 1day. These findings suggest that KDML105's prominent responses to As stress involve sequestering AsIII in vacuoles through the up-regulation of the OsABCC1 gene in the roots, along with detoxifying excessive stress in the leaves through proline accumulation. These responses could serve as valuable traits for selecting As-tolerant rice varieties.

Si-enriched biochars improved soil properties, reduced Cd bioavailability while enhanced Cd translocation to grains of rice.

Biochar and silicon (Si) have been widely considered to play an important role in mitigating cadmium (Cd) toxicity. In this study, wild-type rice (WT, high-Si) and Si-deficient mutant rice (lsi1, low-Si) were used as raw materials to prepare biochar at 500℃; the Si concentrations of high- and low-Si biochar were 15.9% and 5.3%, respectively. The impacts of different application rates (0%, 2%, 4%) of high- and low-Si biochars on soil chemical properties, Si and Cd fractions and availability, Cd absorption, and translocation were investigated. The results showed that both types of biochars increased soil pH, soil available nitrogen, and available phosphorus and potassium; and promoted Si uptake and plant growth of rice. Soil available Si, CaCl2-Si, acetic-Si, H2O2-Si, oxalate-Si, and Na2CO3-Si were also increased by biochar supply, especially for high-Si biochar treatments. In addition, both types of biochars had no effects on soil total Cd, but reduced soil available Cd by 2-17% in early season 2022, and reduced oxidizable Cd and residual Cd. Biochar application did not influence Cd concentrations in roots, stems, and leaves, but significantly increased Cd uptake and transport from stems and leaves to grains. The results suggested that Si-rich biochar could improve soil nutrients, change soil Si/Cd fractions and availability, promote rice growth but increase the risk of Cd toxicity in grains, indicating the complex of straw biochar in remediating Cd-contaminated paddy soil.

Photosynthetic mechanisms underlying NaCl-induced salinity tolerance in rice (Oryza sativa)

BackgroundSalinity stress is an environmental constraint that normally develops concurrently under field conditions, resulting in drastic limitation of rice plant growth and grain productivity. The objective of this study was to explore the alleviating effects of NaCl pre-treatment on rice seedlings as well as the salt tolerance mechanisms by evaluating morph-physiological traits.ResultsVariety Huanghuazhan, either soaked in distilled water or 25 mg/L Prohexadione calcium (Pro-Ca), were first hardened with varying concentrations of NaCl solutions (0 and 50 mM NaCl), and then subjected to varying degrees of salt stress (0 and 100 mM NaCl), indicated by S0, S1, S2 and S3, respectively. Growth analysis suggested that NaCl-pretreatment improved the root/shoot ratio in water-soaked rice plant at DAP 0. Data related to the reaction center density, photosynthetic electron transport efficiency, trapping efficiency were compared before (CK) using performance Index (PIabs). Compared to S2 (Pro-Ca-S2) treatment, PIabs did not show any difference with plants pre-treated with NaCl (S3 or Pro-Ca-S3). Rather than PIabs, significant difference was found in photosynthetic electron transport efficiency (ΨEo). The ΨEo value in Pro-S2 was significantly lowered as compared to Pro-S3 treatment at DAP 7, and the decrease rate was about 6.5%. Correlation analysis indicated leaf PIabs was weak correlated with plant biomass while the quantum yield for reduction of the PSI end electron acceptors, trapped energy flux per reaction center and PSII antenna size displayed strong positive correlation with biomass. Additional analysis revealed that 100 mM NaCl significantly reduced leaf linear electron flux under low-light conditions, regardless of whether seedlings had been pre-treated with 50 mM NaCl or not.ConclusionsNaCl-induced salt tolerance was related to the robust photosynthetic machinery.

Effect of Application of Endophytic Bacteria on the Development of Bacterial Leaf Blight Disease, Growth, and Production of Rice Plants

Bacterial leaf blight can be found in various stages of rice plant growth, so this disease can affect the growth and production of rice plants. This research aims to determine the effect of applying an endophytic bacterial formula to rice seedlings on the development of HDB disease, growth, and production of rice plants. This study used 4 isolates of endophytic bacteria (isolates BE-KH1, BE-M2, BE-M3, and BE-S5) which were formulated in coconut water waste + peptone. The results of the study showed that the application of the endophytic bacterial formula to rice seedlings was able to reduce the severity of bacterial leaf blight by 54,5 – 63,2% and had an effect on growth, full grain weight, and rice grain weight in rice plants inoculated with Xanthomonas oryzae pv. oryzae bacteria.

Potential of Endophytious Fungi from Rice Plant Local Genotype Kuantan Singingi in Improving Rice Plant Growth

This study aims to investigate the potential of endophytic fungi derived from local genotypes of Pulut Karate and white Sisgam rice plants to enhance the growth of rice plants. The research was conducted in a laboratory setting using an experimental approach. Initially, the endophytic fungi were isolated from the rice plants of Pulut Karate and white lionfish genotypes, which were previously collected in another research project. These fungi were then cultured on PDA media and incubated for seven days. Subsequently, the germination and growth of rice seedlings inoculated with each endophytic fungus isolate were examined. The growth parameters of the rice seedlings, including seed height, root length, and number of leaves, were carefully observed. The findings of this research were presented in the form of data and images. The results indicate that the seed germination rate ranged from 90% to 100%. Among all the isolates, isolate PB03 demonstrated the most significant impact on rice plant growth, as evidenced by its taller plant height (8.03 cm) and longer root length (6.27 cm) compared to the other isolates and the control group.