Rice Leaffolder Research Articles

Spesies Serangga Hama Padi dan Jagung serta Intensitas Serangannya di Kabupaten Lahat

Insect pest attack on rice (Oryza sativa L.) and maize (Zea mays L.) could be potential in decrease production. This study aims to determine the insect pest species of rice and maize and attack intensity in Lahat District. This study was conducted using survey methods. The systematic random sampling methodology was adopted for sample population identification. Surveys were conducted in 3 subdistricts (Jarai, Pajar Bulan and Sukamerindu). Found three rice insect pests species. The third species was rice stem borer (Scirpophaga incertulas), leaf folder (Cnaphalocrosis medinalis) and rice bug (Leptocorisa sp). Found one maize insect pest species was fall armyworm (Spodoptera frugiperda). S. incertulas has attacked in 7 locations with low-intensity, Leptocorisa sp has attacked in 2 locations with low-intensity, C. medinalis has attacked in 1 location with low-intensity and S. frugiperda has attacked in 4 locations with low-intensity. Rice stem borer and fall armyworm can attack crop from the vegetative to generative phase.

Genetic polymorphism and plant growth promotion traits of potent fungal entomopathogens of rice leaf folder.

Entomopathogenic fungal biocides are preferred for environment friendly sustainable management of insect pests due to their host specificity and harmlessness to non-target insects. Plant growth promotion (PGP) functions of the entomofungi are also important attributes but hitherto insignificantly explored. Therefore, virulence of 17 natural fungal entomocides (Cordyceps, Beauveria, Metarhizium, Nomuraea, Fusarium, Verticillium, Trichoderma and Paecilomyces spp.) were evaluated for pathogenicity against five rice pests (brown plant hopper (Nilaparvata lugens) and green leaf hopper (Nephotettix virescens) nymphs, leaf folder (Cnaphalocrosis medinalis) and yellow stem borer (Scirpophaga incertulas) larvae and swarming caterpillar (Spodoptera mauritia), respectively), and PGP traits of the potent leaf folder pathogens. Among the fungi, only the leaf folder pathogens (3 isolates each of Beauveria and Metarhizium spp.) infected > 50% (80-90%) larvae but other fungi were ineffective as infected < 50% (0-47%) insects. Besides, the leaf folder pathogens exhibited diverse PGP traits such as organic/inorganic phosphate solubilization (104.7-236.4µg/ml), and siderophore, ammonia, hydrogen cyanide (HCN), indole production etc. Restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), simple sequences repeat (SSR) and internal transcribed spacers (ITS) analysis ascertained strain identity and genetic (inter and intra-specific) diversity among the potent biocides Beauveria and Metarhizium spp. The virulent natural fungal pathogens of rice pests with polyvalent PGP traits may be prospected for rice growth promotion and biocontrol of leaf folder.

Natural Farming Boosts Coccinelid Abundance and Suppressing Rice Leaf Folder (Cnaphalocrosis Medinalis) in Rice in Indo-Burma Region.

&nbsp; Ecological engineering cropping systems were developed for aroma (Pusa Basmati-1121, Badshah bhog, and Gandhi Biron) and non-aroma rice varieties (Tripura Chikan Dhan, Tripura Nirogi Dhan, and Tripura Sharat Dhan). 2017-18 and 2018-19 field experiments were performed at the ICAR Research Complex for NEH Region, Mizoram Centre, Kolasib, Mizoram. Ecological engineering parameters such as pest defender ratio, occurrence ratio (OR) of entomophages, preference ratio (PR) of pests, biodiversity indices (species diversity, richness, and evenness) of entomophages and pests, and cost benefit ratio (CBR) were assessed for all cropping systems. The population of Micraspis crocea (Mulsant) on rice was most affected by rice (Gomati Dhan) + Pusa Basmati-1121, followed by rice + Gandhi Biron and rice + Badsha Bhog. Rice + Tripura Nirogi Dhan, Rice + Tripura Sharat Dhan, and Rice + Tripura Chikon Dhan border systems impacted rice's moderate entomophage population more than rice alone. rice + Pusa Basmati-1121, rice + Gandhi Biron, and rice + Badsha Bhog border cropping system reduced leaf folder (Cnaphalocrosis medinalis (Guenee), population and damage on rice compared to rice alone. rice + Tripura Nirogi Dhan, rice + Tripura Sharat Dhan, and rice + Tripura Chikon Dhan border cropping system showed moderate pest population and damage. Pest defender ratio was highest on rice + Pusa Basmati-1121 (1: 6.23 and 1: 4.08), rice + Gandhi Biron (1: 5.76 and 1: 3.83) and rice + Badsha Bhog (1: 5.26 and 1: 3.35) border system compared to rice + Tripura Nirogi Dhan (1: 4.00 and 1: 2.65), rice +Tripura Sharat Dhan (1: 3.09 and 1: 2.31), and rice + Tripura Chikon Dhan (1: 2.90 and 1: 1.83) border system and rice alone (1:1.75 and 1: 1.68) border systems. Rice + Pusa Basmati-1121, rice + Gandhi Biron, and rice + Badsha Bhog border systems had the highest OR of entomophages, lowest PR of pests, highest yield, and highest CBR compared to rice + Tripura Nirogi Dhan, Tripura Sharat Dhan, and Tripura Chikon Dhan border cropping systems and rice alone. &nbsp; &nbsp

Impact of Abiotic Factors on the Incidence of Insect Pests in Rice

The investigation on impact of abiotic factors on incidence of insect pests in rice was carried out during Kharif 2021. The highest and lowest Rice Yellow Stem Borer incidence was recorded at vegetative stage in the form of dead heart at 31st Standard Meteorological Weeks (SMW) and 40th SMW and it was 16.48% and 1.10%, respectively, whereas at reproductive stage the highest and lowest incidence was recorded in the form of white ear at 42nd SMW and 41th SMW i.e., 18.12% and 16.75%, respectively. The highest Rice leaf folder incidence was recorded in 36th SMW (16.25%) and lowest in 44th SMW (1.31%). The highest and lowest Rice Gundhi bug mean population was recorded in 39th SMW (10.30 bugs/hill) and 44th SMW (1.31 bugs/hill). The highest and lowest Brown Plant Hopper (BPH) mean population was recorded in 38th SMW and 41st SMW and it was10.80 BPH/hill and 1.60 BPH/hill, respectively. The yellow stem borer showed significant positive correlation with relative humidity whereas, non-significant positive correlation with minimum temperature, maximum temperature, rainfall and sunshine. Rice leaf folder population showed significant positive correlation with minimum temperature and relative humidity. Whereas, positive and non-significant correlation with maximum temperature and rainfall. However, negative and non-significant correlation with sunshine. Gundhi bug showed a non-significant positive correlation with Relative humidity, Rainfall and sunshine. However, negative and non-significant correlation with minimum temperature and maximum temperature. BPH population showed significant positive correlation with minimum temperature and relative humidity, however positive and non-significant correlation with maximum temperature and rainfall. Whereas, negative and non-significant correlation with Sunshine.

Determination of chlorantraniliprole 18.5% SC in the paddy ecosystem and its risk assessment

Chlorantraniliprole belongsto theanthranilic diamide group is widely used against broad range of lepidopteron pests in a variety of vegetable and rice pests includingyellow rice stem borer and leaf folder. Supervised field trials were conducted duringRabi (2018–2019) and Kharif (2019) to evaluate the dissipation pattern and risk assessment of chlorantraniliprole 18.5% SC in paddy ecosystem following foliar application at 30 and 60 g a.i. ha-1 in two different cropping seasons.Modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) technique was used for the extraction of CAP residues with acetonitrile and determined by LC–MS/MS (ESI +).The limit of quantification (LOQ) was 0.01 µg g−1 for paddy leaf, straw, husk, and brown rice, respectively and 0.005 µg g−1 for soil. The average recoveries obtained were 84.30–88.92% from paddy leaf, 94.25–97.81% from straw, 90.21–93.38% from husk, 93.57–96.40% from brown rice and 89.93–91.14% from soil. The residues in paddy leaf dissipated within 35–40 days with a half-life of 4.33–5.07 days in Rabi and 3.92–4.86 days in Kharif at 30 and 60 g a.i. ha−1, respectively. The residues in soil dissipated within 15–21 days with a half-life of 14.44–15.75 days in Rabi and 13.33–14.44 days in Kharif at respective doses. At harvest chlorantraniliprole residues were not detected in straw, husk, and brown rice. The dietary risk of paddy leaf (green fodder) for cattle was found safe for consumption as the hazard index is less than one. Soil ecological risk assessment was found to be less than one (RQ < 0.1) for earthworms (Eisenia foetida) and arthropods (Aphidiusrhopalosiphi). The presentmethod could be useful inthe analysis ofchlorantraniliproleresidues in different cereals and vegetable crop ecosystems and application at recommended dose is safe for the final produce at harvest.

Evaluation of Botanicals Insecticide against Stem Borer Scirpophaga incertulas (Walker) Leaf Folder (Cnaphalocrosis medinalis) and Green Leafhopper and in Rice

A field experiment was conducted during the kharif season of 2020-21 and 2021-22 at Crop Research station, Masodha, Ayodhya, India to evaluate the efficacy of insecticide-botanicals against rice stem borer, green leafhopper and leaf folder. The experiment was comprising Four combination modules/treatments consisting of three insecticides Chlorantraniliprole 20% SC, Cartap hydrochloride 50% SC and Triflumezopyrim 10% SC, one commercial neem formulation - Neemazal and two plant oils - Neem and Eucalyptus oil procured were compared along with untreated control (only water spray). The lowest stem borers incidence (1 %) of in all insecticides treatment module recorded as compared to 14.8% in untreated control. Among modules, all insecticides module was found to be the best with 1.0% mean white ear damage followed by neemazal, eucalyptus oil and cartap hydrochloride module with 5.3% WE. Green leafhopper infestation was all insecticides combination was the most effective treatment showing mean population of 110.7/10 hills followed by all botanical combination (120.7) and were superior to control (158.7/10 hills). The mean leaf Folder damage (1.8%) was recorded from all insecticides module followed by treatment with neemazl (? %), Eucalyptus oil (? %), and Triflumezopyrim 10% SC (3.9% DL) when compared to untreated control (14.5% )4. Grain yield all insecticides treatment - Chlorantraniliprole, Cartap hydrochloride, Triflumezopyrim recorded the highest grain yield of 5212.0 kg/ha with 95.79% increase over control (IOC) followed by neemazal, neem oil and cartap hydrochloride with 4500 kg/ha (69.04% IOC). All the treatments were significantly superior to control plot which showed a yield of 2662 kg/ha.

Identification and characterization of ABC proteins in an important rice insect pest, Cnaphalocrocis medinalis unveil their response to Cry1C toxin

Rice leaffolder (Cnaphalocrocis medinalis) is an important insect pest in paddy fields. Due to their essential role in the physiology and insecticidal resistance, ATP-binding cassette (ABC) proteins were studied in many insects. In this study, we identified the ABC proteins in C. medinalis through genomic data and analyzed their molecular characteristics. A total of 37 sequences with nucleotide-binding domain (NBD) were identified as ABC proteins and belonged to eight families (ABCA-ABCH). Four structure styles of ABC proteins were found in C. medinalis, including full structure, half structure, single structure, and ABC2 structure. In addition to these structures, TMD-NBD-TMD, NBD-TMD-NBD, and NBD-TMD-NBD-NBD were found in C. medinalis ABC proteins. Docking studies suggested that in addition to the soluble ABC proteins, other ABC proteins including ABCC4, ABCH1, ABCG3, ABCB5, ABCG1, ABCC7, ABCB3, ABCA3, and ABCC5 binding with Cry1C had higher weighted scores. The upregulation of ABCB1 and downregulation of ABCB3, ABCC1, ABCC7, ABCG1, ABCG3, and ABCG6 were associated with the C. medinalis response to Cry1C toxin. Collectively, these results help elucidate the molecular characteristics of C. medinalis ABC proteins, pave the way for further functional studies of C. medinalis ABC proteins, including their interaction with Cry1C toxin, and provide potential insecticide targets.

Stage Specific Life Table of Rice Leaffolder, Cnaphalocrocis medinalis (Guen.) at Different Temperature Regimes

The change in air temperature will influence the insect behavior, physiology and population dynamics of insects as they are poikilothermic. Global temperature has increased by 1.09°C during 2001-2020 when compared to 1850-1900. It is projected that the temperature will further increase by 5.7°C under high emission scenario, if mitigation strategies are not adopted. Increase in temperature would affect the physiology and population dynamics of the insects. A study was undertaken to understand the effect of different temperature regimes on rice leaffolder. Survival fraction decreased with increasing temperatures as more successful development was at lower temperature regimes. Apparent mortality increased towards the higher temperature regimes as the insects cannot tolerate high temperature stress. The Mortality Survivor Ratio (MSR) revealed that the population increase would be more at higher temperature regimes as the MSR remained higher at higher temperature regimes. The Indispensable Mortality was lesser under higher temperature regimes as the number of adults emerged in the high temperature regime was less. Generally, K - values increased with increasing temperature. It indicates that the insects which happened to live under higher temperature regimes were reproduction oriented as most of the energy was spent in reproduction rather than for living longer time.

Insect Herbivory on Main Stem Enhances Induced Defense of Primary Tillers in Rice (Oryza sativa L.).

Clonal plants are interconnected to form clonal plant networks with physiological integration, enabling the reassignment as well as sharing of resources among the members. The systemic induction of antiherbivore resistance via clonal integration may frequently operate in the networks. Here, we used an important food crop rice (Oryza sativa), and its destructive pest rice leaffolder (LF; Cnaphalocrocis medinalis) as a model to examine defense communication between the main stem and clonal tillers. LF infestation and MeJA pretreatment on the main stem for two days reduced the weight gain of LF larvae fed on the corresponding primary tillers by 44.5% and 29.0%, respectively. LF infestation and MeJA pretreatment on the main stem also enhanced antiherbivore defense responses in primary tillers: increased levels of a trypsin protease inhibitor, putative defensive enzymes, and jasmonic acid (JA), a key signaling compound involved in antiherbivore induced defenses; strong induction of genes encoding JA biosynthesis and perception; and rapid activation of JA pathway. However, in a JA perception OsCOI RNAi line, LF infestation on main stem showed no or minor effects on antiherbivore defense responses in primary tillers. Our work demonstrates that systemic antiherbivore defense operate in the clonal network of rice plants and JA signaling plays a crucial role in mediating defense communication between main stem and tillers in rice plants. Our findings provide a theoretical basis for the ecological control of pests by using the systemic resistance of cloned plants themselves.

Bio-efficacy of chlorantraniliprole 0.4% gr against Cnaphalocrocis medinalis and Scirpophaga incertulas in rice (Oryza sativa)

With the widespread adoption of high yielding rice (Oryza sativa L.) varieties and accompanying changes in the cultural practices, rice leaf folder [Cnaphalocrocis medinalis (Guenee)] and yellow stem borer [Scirpophaga incertulas (Walker)] has attained the status of major pests and influence the yield if proper timely management practices are not adopted. For effective management of these pests, identification of new molecules with novel mode of action, low toxicity to non-target insects and environmental safety is required for sustainable IPM. Chlorantraniliprole 0.4% gr (Ferterra 0.4% gr) is an anthranillic diamide insecticide in granular form for the control of lepidopterous pests in rice. A field experiment was conducted to evaluate the efficacy of chlorantraniliprole 0.4% gr (Ferterra 0.4% gr) against rice leaf folder and yellow stem borer during rainy (kharif) seasons 2017–19. The study revealed that broadcast application of chlorantraniliprole 0.4% gr @40 g a.i./ha at tillering stage was found effective and best insecticidal treatment in reducing rice leaf folder and yellow stem borer infestation with no phytotoxicity symptoms and increased grain yield. Chlorantraniliprole 0.4% gr @40 g a.i./ha was also safer to the natural enemies of the rice ecosystem. Post-harvest residual analysis of chlorantraniliprole 0.4% gr was found below the limit of quantification, i.e. 0.01 ppm in rice grains, soil, paddy straw and husk at recommended and double of the recommended dose indicating its safety to human, animals and environment.

Defensive Role of Fibre Fractions in Rice Genotypes against Rice Leaf Folder (Guenee)

Defensive Role of Fibre Fractions in Rice Genotypes against Rice Leaf Folder (Guenee)

MANAGEMENT OF INSECT PEST IN RICE SEEDBED USING A NOVEL RECTANGULAR HAND NET (RHN) FOR INSECTICIDE FREE SEEDLINGS

Insect pest first attacks rice in seedbed generally 7-9 days after sprouted seed sowing. At this early sensitive stage, traditional sweep net (round hand net) is not suitable to collect insects because it injured tender rice seedlings and its insect catching efficiency is also lower than rectangular hand net. To alleviate this problem, developed a novel rectangular hand net and evaluated the efficacy for managing insect pest in rice seedbeds. Newly developed hand net consists of a rectangular frame that includes 4 mm GI wire, and the frame length and width 50 cm and 20 cm, respectively. It also consists of a plastic pipe that length 100 cm, radious 0.75 inch, and market available white colour mosquito net, which length from the frame is 80 cm. Methods of application is rapidly walking with RHN (Fig. 2) around the seedbed (model seedbed one-meter width and length depends on land condition). After sweeping full seedbed harmful insect has been destroyed and beneficial insect released back in the same field. RHN caught maximum number of major populations YSB, rice hispa, BPH, rice leaf folder, GH, etc., and remained under control. RHN caught beneficial insect (19.60 %) compare to harmful insect pest (80.39 %). In Aus season 2022, 20 m2 area BRRI dhan48, BRRI dhan98, BRRI dhan85 seedbed tenure caught insect pest (87.78 %) and natural enemy (12.22 %). Same year T. Aman season BRRI dhan76 and BRRI dhan52 seedbed period insect pest caught (71.18%) and natural enemy (28.82 %). Both seasons conducted this experiment at BRRI, Barishal Charbadna and Sagordi farm.

Evaluation of host plant resistance against the rice leaf folder (Cnaphalocrosis medinalis) and yellow stem borer (Scirpophaga incertulus) through genotypic screening of rice

Evaluation of host plant resistance against the rice leaf folder (Cnaphalocrosis medinalis) and yellow stem borer (Scirpophaga incertulus) through genotypic screening of rice

Evaluation of rice genotypes against rice leaf folder, Cnaphalocrosis medinelis Guenee in Koshi region of Bihar

Evaluation of rice genotypes against rice leaf folder, Cnaphalocrosis medinelis Guenee in Koshi region of Bihar

Bio-efficacy of insecticides against rice yellow stemborer and leaf folder

Bio-efficacy of insecticides against rice yellow stemborer and leaf folder

Species composition, occurrence pattern and seasonal incidence of rice leaf folder (Cnaphalocrocis spp.) on kharif rice under Terai agro-ecological conditions of Northern Parts of West Bengal

Species composition, occurrence pattern and seasonal incidence of rice leaf folder (Cnaphalocrocis spp.) on kharif rice under Terai agro-ecological conditions of Northern Parts of West Bengal

Increased efficacy of entomopathogenic fungus, Beauveria bassiana (Balsamo) Vuillemin (Ascomycota: Hypocreales) against leaf folder, Cnaphalocrocis medinalis Guenee (Lepidoptera: Pyralidae), incidence on silica enriched rice crop

A possible synergistic effect between biological control agent Beauveria bassiana (Bb) and potassium silicate (K2Sio) for control of leaf folder on rice was investigated at Agricultural Research Station, Nellore (ANGRAU, Guntur, Andhra Pradesh) under field conditions. Three sequential Silicon application in the form of potassium silicate (KSil) was given as foliar spray @ 80 mg/l, when leaf folder population density reached to ETL, B. bassiana @ 1.3x106 conidia/ml application was carried out. Two sequential applications of potassium silicate (two weeks after transplantation and at active tillering stage) + B. bassiana caused leaf folder population reduction of 42.80 per cent over control at 8 days after spraying. This reduction was significantly higher than the mortalities caused by the two single treatments alone (B. bassiana: 17.55 %; Ksil @ 2sprayes: 8.83). This study providing strong evidence of a synergistic effect of the combination treatment on leaf folder mortality. Similarly, three sequential applications of potassium silicate (two weeks after transplantation, at active tillering stage and PI stage) + B. bassiana caused 41.88 % reduction in leaf older population over control at 8 days after spraying, whereas three sequential applications of potassium silicate and application of B. bassiana alone caused 20.57 and 17.55 % reduction in leaf folder incidence, respectively. Indicates application of potassium silicate and B. bassiana alone did not gave as satisfactory results as used in combinations. In the present study the sequential applications of Potassium silicate + B. bassiana gave more promising control of rice leaf folder compared to the application of B. bassiana alone, proving the synergistic effect of the potassium silicate + B. bassiana combination on leaf folder incidence.

SEASONAL INCIDENCE OF MAJOR INSECT PESTS OF RICE IN KRISHNA WESTERN DELTA REGION OF GUNTUR DISTRICT

The seasonal occurrence of major insect pests of rice in Guntur district of Andhra Pradesh during kharif 2021-22 was studied in Krishna Western Delta area in 90 farmers fields of 10 mandals (Bapatla, Kakumanu, Ponnur, Tsunduru, Amruthaluru, Vemuru, Tenali, Duggirala, Repalle and Nagaram). Fortnightly observations on pest population in parallel relation with meteorological parameters revealed the highest leaf damage due to rice leaf folder Marasmia medinalis (Crambidae: Lepidoptera)during second fortnight of September (2.95%) when the crop was more succulent. The incidence of yellow stem borer Scirpophaga incertulas (Crambidae: Lepidoptera) begun appearing during first fortnight of August with highest percent damage during second fortnight of September causing 2.62 percent dead hearts. The peak population of brown planthopper Nilaparvata lugens (Delphacidae: Hemiptera) was observed during first fortnight of October (4.97 hoppers/hill) when the crop was in panicle initiation stage.

Gut bacterial communities and their assembly processing in Cnaphalocrocis medinalis from different geographic sources.

The insect gut harbors numerous microorganisms that may have functions in development and reproduction, digestion, immunity and protection, and detoxification. Recently, the influence factors on gut microbiota were evaluated in the rice leaffolder Cnaphalocrocis medinalis, a widespread insect pest in paddy fields. However, the relationship between gut microbiota composition and geography is poorly understood in C. medinalis. To reveal the patterns of C. medinalis gut bacterial communities across geographic sources and the ecological processes driving the patterns, C. medinalis were sampled from six geographic sources in China, Thailand, and Vietnam in 2016, followed by gut bacterial 16S ribosomal RNA gene sequencing. A total of 22 bacterial phyla, 56 classes, 84 orders, 138 families, 228 genera, and 299 species were generated in C. medinalis from six geographic sources. All alpha diversity indices differed among the samples from different geographic sources. Analysis of similarity (ANOSIM) and permutational multivariate analysis of variance (PERMANOVA) both revealed significant differences in the gut microbiota of C. medinalis from six geographic sources. A total of 94 different taxa were screened as indicators for the gut microbiota of C. medinalis from six geographic sources by linear discriminant analysis effect size (LEfSe). The gene ontology (GO) pathways of the gut microbiota in C. medinalis differed among geographic sources. In total, the bacterial communities within geographic sources were mainly determined by stochastic processes, and those between geographic sources were mainly determined by deterministic processes. This study elucidates that geography plays a crucial role in shaping the gut microbiota of C. medinalis. Thus, it enriches our knowledge of gut bacteria in C. medinalis and sheds light on the mechanisms underlying C. medinalis gut microbial shifts across geography.

Biocontrol Potential of PeBL2, a Novel Entomopathogenic Bacterium from Brevibacillus laterosporus A60, Induces Systemic Resistance against Rice Leaf Folder Cnaphalocrocis exigua (Butler) in Rice (Oryza sativa L.)

The dangerous insect pest known as rice leaf folder Cnaphalocrocis exigua (Butler), which reduces rice output globally, twists and feeds on the young rice plant's leaves. Protein elicitors are hypothesized to be biological components that promote rice in becoming herbivore resistant. The evolving elicitor protein PeBL2, obtained from Brevibacillus laterosporus A60, was tested for biocontrol against C. exigua. Four distinct PeBL2 doses (74.23, 45.53, 22.26, and 11.13 μg mL-1) were assigned to evaluate the impact of PeBL2 on immature growth, survivability, and lifespan. Adult reproductive efficiency and the interaction between the pest and the disease were assessed against C. exigua. Further, the assessment of active compounds in PeBL2 with multi-acting entomopathogenic effects investigated the direct correlations of PeBL2 with temperature and climatic change in plants of rice (Oryza sativa L.). When compared to controls, PeBL2 treatments reduced the growing population of second- and third-generation C. exigua. Cnaphalocrocis exigua colonized control plants faster than PeBL2-treated O. sativa plants in a host selection test. PeBL2 doses delayed the development of the larval stage of C. exigua. PeBL2-treated seedlings generated less offspring than control seedlings, identical to fecundity. Trichomes and wax formation on PeBL2-treated leaves generated an adverse environment for C. exigua. PeBL2 altered the surface topography of the leaves, preventing colonization and reducing C. exigua reproduction. PeBL2-treated O. sativa seedlings exhibited somewhat increased amounts of jasmonic acid (JA), salicylic acid (SA), and ethylene (ET). Systemic defensive processes also included the activation of pathways (JA, SA, and ET). Following these results versus C. exigua, the use of PeBL2 in an agroecosystem with integrated pest management and biocontrol appears to be reasonable. These findings shed new light on a cutting-edge biocontrol technique based on B. laterosporus A60.