Cytogenetic and DNA cytophotometric image analyses were conducted for the identification and labelling of nine (9) genotypes of commercial hybrids of sugarcane,Saccharum officinarum, include GT 54-9, Bo 10, A 63-20, NCo 310, Co 413, G 75-393, G 88-68, G 67-55 and Co 301. These genotypes were locally available in Sabahia Sugar Crops Research Station, Alexandria, Egypt. Squash of root-tips, of 10–14 days-old sprouts, fixed in Carnoy’s solution was stained with Feulgen reagent and the cell image was determined using ComputerizedImage Analysis in Scientific Application (CIASA) system. The study showed that DNA content ranged from 200–2000 Arbitrary Units, detecting over six ploidy level (>6C) in three genotypes namely, A 63-20, NCo 310 and Co 413. The genotypes GT 54-9, Bo 10, and Co 301 contained up to 5C, and the remaining four genotypes contained only up to 4C. Cytogenetic examination revealed different numbers ofnucleoli per nucleus of the root-tip squash; three to four nucleoli in genotypes Bo 10 and Co 301, two in genotypes G 75-393 and G 67-55, and six to more than eight in genotypes GT 54-9, NCo 310 and Co 413. This indicates that these genotypes were obtained from hybridization and or translocation of ribosomal DNA cistrons. Genotypes NCo 310 and Co 413 displayed eight nucleoli.Mitotic indices ranged from 2.1 (G 88-68) to 4.6 (Co 413). A high degree of heterochromatin at interphase was observed in A 63-20 and G 67-55; reflecting low transcriptional activity. The mitotic index is an accurate differential measure of cell proliferation, suggesting different kinetic levels of the investigated genotypes, which can be ranked descending in the order Co 413, BO 10, GT 54-9,G 67-55,NCo 310,Co 301, A 63-20,G 75-393 and G 88-68.Cytological examination revealed several aberrations in the Pollen Mother Cells (PMCs) in various stages of meiosis. The genotypes NCo 310 and Co 413 showed abnormal behaviour of chromosomes, giving evidence of polyploidy. The results of this study show that the genetic and cytogenetics features of the nine investigated genotypes reflect genotype-specific characteristics including DNA content (ploidy level), estimated volume of nucleoli per cell (No. x Size), mitotic index and transcriptional activity (heterochromatin) that may offer means of sugarcane clonal genotypes or varietal verification and avoidance of mislabelling for use as parental lines in breeding programmes and sugarcane improvement.