The separation and determination of vitamin B 6 forms (pyridoxine, pyridoxal, pyridoxamine, pyridoxal-phosphate and pyridoxamine-phosphate) with paper electrophoresis and starch column electrophoresis are described. Procedures for separating riboflavin derivatives (riboflavin, FMN and FAD) and nicotinamide derivatives (nicotinamide, DPN and TPN) by paper electrophoresis are reported. A separation of all the above compounds from one mixture has also been obtained.