Rhythmic Production Research Articles

The Neural Substrate of Predictive Motor Timing in Spinocerebellar Ataxia

The neural mechanisms involved in motor timing are subcortical, involving mainly cerebellum and basal ganglia. However, the role played by these structures in predictive motor timing is not well understood. Unlike motor timing, which is often tested using rhythm production tasks, predictive motor timing requires visuo-motor coordination in anticipation of a future event, and it is evident in behaviors such as catching a ball or shooting a moving target. We examined the role of the cerebellum and striatum in predictive motor timing in a target interception task in healthy (n = 12) individuals and in subjects (n = 9) with spinocerebellar ataxia types 6 and 8. The performance of the healthy subjects was better than that of the spinocerebellar ataxia. Successful performance in both groups was associated with increased activity in the cerebellum (right dentate nucleus, left uvula (lobule V), and lobule VI), thalamus, and in several cortical areas. The superior performance in the controls was related to activation in thalamus, putamen (lentiform nucleus) and cerebellum (right dentate nucleus and culmen-lobule IV), which were not activated either in the spinocerebellar subjects or within a subgroup of controls who performed poorly. Both the cerebellum and the basal ganglia are necessary for the predictive motor timing. The degeneration of the cerebellum associated with spinocerebellar types 6 and 8 appears to lead to quantitative rather than qualitative deficits in temporal processing. The lack of any areas with greater activity in the spinocerebellar group than in controls suggests that limited functional reorganization occurs in this condition.

A circadian rhythm regulating hyphal melanization in Cercospora kikuchii

Many metabolic and developmental processes in fungi are controlled by biological rhythms. Circadian rhythms approximate a daily (24 h) cycle and have been thoroughly studied in the model fungus, Neurospora crassa. However relatively few examples of true circadian rhythms have been documented among other filamentous fungi. In this study we describe a circadian rhythm underlying hyphal melanization in Cercospora kikuchii, an important pathogen of soybean. After growth in light or light : dark cycles, colonies transferred to darkness produced zonate bands of melanized hyphae interspersed with bands of hyaline hyphae. Rhythmic production of bands was remarkably persistent in the absence of external cues, lasting at least 7 d after transfer to darkness, and was compensated over a range of temperatures. As in N. crassa, blue light but not red light was sufficient to entrain the circadian rhythm in C. kikuchii, and a putative ortholog of white collar-1, one of the genes required for light responses in N. crassa, was identified in C. kikuchii. Circadian regulation of melanization is conserved in other members of the genus: Similar rhythms were identified in another field isolate of C. kikuchii as well as field isolates of C. beticola and C. sorghi, but not in wild-type strains of C. zeae-maydis or C. zeina. This report represents the first documented circadian rhythm among Dothideomycete fungi and provides a new opportunity to dissect the molecular basis of circadian rhythms among filamentous fungi.

Cognitive Demand of Human Sensorimotor Performance During an Extended Space Mission: A Dual-Task Study

Two previous single-case studies found that the dual-task costs of manual tracking plus memory search increased during a space mission, and concluded that sensorimotor deficits during spaceflight may be related to cognitive overload. Since dual-task costs were insensitive to the difficulty of memory search, the authors argued that the overload may reflect stress-related problems of multitasking, rather than a scarcity of specific cognitive resources. Here we expand the available database and compare different types of concurrent task. Three subjects were repeatedly tested before, during, and after an extended mission on the International Space Station (ISS). They performed an unstable tracking task and four reaction-time tasks, both separately and concurrently. Inflight data could only be obtained during later parts of the mission. The tracking error increased from pre- to in flight by a factor of about 2, both under single- and dual-task conditions. The dual-task costs with a reaction-time task requiring rhythm production was 2.4 times higher than with a reaction-time task requiring visuo-spatial transformations, and 8 times higher than with a regular choice reaction-time task. Long-term sensorimotor deficits during spaceflight may reflect not only stress, but also a scarcity of resources related to complex motor programming; possibly those resources are tied up by sensorimotor adaptation to the space environment.

Perception–production relationships and phase correction in synchronization with two-interval rhythms

Two experiments investigated the effects of interval duration ratio on perception of local timing perturbations, accuracy of rhythm production, and phase correction in musicians listening to or tapping in synchrony with cyclically repeated auditory two-interval rhythms. Ratios ranged from simple (1:2) to complex (7:11, 5:13), and from small (5:13=0.38) to large (6:7=0.86). Rhythm production and perception exhibited similar ratio-dependent biases: rhythms with small ratios were produced with increased ratios, and timing perturbations in these rhythms tended to be harder to detect when they locally increased the ratio than when they reduced it. The opposite held for rhythms with large ratios. This demonstrates a close relation between rhythm perception and production. Unexpectedly, however, the neutral "attractor" was not the simplest ratio (1:2=0.50) but a complex ratio near 4:7 (=0.57). Phase correction in response to perturbations was generally rapid and did not show the ratio-dependent biases observed in rhythm perception and production. Thus, phase correction operates efficiently and autonomously even in synchronization with rhythms exhibiting complex interval ratios.

Xenopus Bsxlinks daily cell cycle rhythms and pineal photoreceptor fate

In the developing central nervous system, the cell cycle clock plays a crucial role in determining cell fate specification. A second clock, the circadian oscillator, generates daily rhythms of cell cycle progression. Although these two clocks interact, the mechanisms linking circadian cell cycle progression and cell fate determination are still poorly understood. A convenient system to address this issue is the pineal organ of lower vertebrates, which contains only two neuronal types, photoreceptors and projection neurons. In particular, photoreceptors constitute the core of the pineal circadian system, being able to transduce daily light inputs into the rhythmical production of melatonin. However, the genetic program leading to photoreceptor fate largely remains to be deciphered. Here, we report a previously undescribed function for the homeobox gene Bsx in controlling pineal proliferation and photoreceptor fate in Xenopus. We show that Xenopus Bsx (Xbsx) is expressed rhythmically in postmitotic photoreceptor precursors, reaching a peak during the night, with a cycle that is complementary to the daily rhythms of S-phase entry displayed by pineal cells. Xbsx knockdown results in increased night levels of pineal proliferation, whereas activation of a GR-Xbsx protein flattens the daily rhythms of S-phase entry to the lowest level. Furthermore, evidence is presented that Xbsx is necessary and sufficient to promote a photoreceptor fate. Altogether, these data indicate that Xbsx plays a dual role in contributing to shape the profile of the circadian cell cycle progression and in the specification of pineal photoreceptors, thus acting as a unique link between these two events.

Musical instrument having a ribbed surface

The invention relates to a musical instrument with which a sound is produced in the same manner as when playing the guiro (Latin American percussion instrument: longish gourd having a regularly notched surface) by scratching with the finger nail or by playing with a playing aid. In contrast to the guiro or also to the percussion instrument called a washboard, sound rows can be made of individual modules or sections. Playing in a constant tempo enables the production of analogous rhythms due to the constructed interval ratios and scales can be produced by combining different frequencies. Particular embodiments of the instrument and of the playing aid made of different materials produce many nuances in sound that challenge the creativity and the curiosity of the player even more. Possible materials include wood, paperboard, plastic, rubber, metal, ceramics and stone.

Neural bases of individual differences in beat perception

When people listen to music, they often move their body in time with the beat. However, people differ widely in their tendency to ‘feel a beat’. Why? Here we combined functional magnetic resonance imaging with a timing task that is diagnostic of individual differences in beat perception and compared the brain activity of individuals who readily perceive an implied beat with those who do not. Activation in auditory and motor areas was correlated with individual differences in beat perception, even when participants performed a timing task in which no behavioral differences occurred. The results support two conclusions. First, a bias toward beat perception is mediated by the activation of cortical circuits involved in rhythm production. Second, some individuals more readily engage these cortical beat-based circuits when making timing judgments than do others.

The effect of oral proficiency on production of rhythm in spontaneous second language (L2) Japanese speech.

This study addresses the question of whether oral proficiency in Japanese second language (L2) speech has a unique correlation with acoustic characteristics of rhythm production that is independent from segments. Among four rhythm measures used (V%, VarcoV, VarcoC, VI‐M), only two measures were different for the spontaneous L2 Japanese speech of beginning and intermediate learners. The interlanguage rhythm of less proficient speakers of Japanese was characterized by lower variability in duration of vocalic stretches (VarcoV) and higher variability in duration of consonantal stretches (VarcoC), p < 0.05. For both VarcoV and VarcoC values, the distribution of the individual speakers’ rhythm scores was much tighter and on target for the intermediate students than for the beginning students. Furthermore, VarcoV values were significantly correlated with number of utterance‐final vowels, and VarcoC values were correlated with number of obstruent clusters. In sum, the findings suggest that rhythmic differences in spontaneous L2 speech have an epiphenomenal nature stemming from the segmental structure of Japanese: the acquisition of mora‐timed rhythm by learners of Japanese seems to be contingent upon the target‐like production of segments which varies with proficiency level of learners.

Contribution of Commissural Projections to Bulbospinal Activation of Locomotion in the In Vitro Neonatal Rat Spinal Cord

Commissural projections are required for left-right coordination during locomotion. However, their role, if any, in rhythm production is unknown. This study uses the neonatal rat in vitro brain stem-spinal cord model to examine the rostrocaudal distribution of locomotor-related commissural projections and study whether commissural connections are needed for the generation of hindlimb rhythmic activity in response to electrical stimulation of the brain stem. Midsagittal lesions were made at a wide range of rostrocaudal levels. Locomotor-like activity persisted in some preparations despite midsagittal lesions extending from C(1) to the mid-L(1) level or from the conus medullaris to the T(12/13) junction. In some preparations, midsagittal lesions throughout the entire spinal cord had no effect on locomotor-like activity if two or three contiguous segments remained intact. Those bridging segments had to include the T(13) and/or L(1) levels. These observations suggested that commissural projections in the thoracolumbar junction region were critical. However, locomotor-like activity was also elicited in preparations with limited midsagittal lesions focused on the thoracolumbar junction (T(12) through L(1) or L(2) inclusive). In other experiments, locomotor-like activity was evoked by bath-applied 5-hydroxytryptamine (5-HT) and N-methyl-d-aspartate (NMDA). Appropriate side-to-side coordination was observed, even when only one segment remained bilaterally intact. Commissural projections traversing the thoracolumbar junction region were most effective. In combination, these results suggest that locomotor-related commissural projections are redundantly distributed along a bi-directional gradient that centers on the thoracolumbar junction. This commissural system not only provides a robust left-right coordinating mechanism but also supports locomotor rhythm generation in response to brain stem stimulation.

Vasopressin: more than just an output of the circadian pacemaker? Focus on “Vasopressin receptor V1a regulates circadian rhythms of locomotor activity and expression of clock-controlled genes in the suprachiasmatic nuclei”

the timing of physiological events is carefully controlled so that incompatible processes do not compete and complementary functions coincide. Given the pivotal role of the hypothalamus in homeostatic regulation, the discovery that a master circadian pacemaker resides in this region was not

Cloning and expression of arylalkylamine N-acetyltranferase-2 during early development and metamorphosis in the sole Solea senegalensis

The arylalkylamine N-acetyltransferase (AANAT) is a key enzyme in the rhythmic production of melatonin. Two Aanats are expressed in teleost fish, one retinal specific, Aanat1, and the other one pineal specific, Aanat2, being the latter the main enzyme responsible of the plasma nocturnal melatonin increase in fish. In anurans melatonin has been involved in metamorphosis through antagonizing thyroid hormone function; however, no available data reports a relationship between melatonin system and metamorphosis in fish. In this study, we have cloned the AANAT2 ( SsAanat2) in a flatfish, Solea senegalensis, and studied its sites of expression and developmental expression pattern by in situ hybridization and Real Time PCR. These studies allowed us to demonstrate a specific signal in the pineal gland of sole larvae from 2 days post-fertilization (dpf), which was evident until post-metamorphosis. Immunohistochemical analysis on the hybridized slides showed that the sole pineal Aanat2 expressing cells corresponded to pineal photoreceptor cells. Real Time PCR was performed in animals kept under natural photoperiod and sampled at different stages from 0 to 21 dpf (including pre-, early-, middle- and late-metamorphic stages) and at midlight (ML) and middark (MD) daytimes. Sole Aanat2 expression was higher at MD than at ML from 2 dpf and at most developmental stages analyzed. The highest AANAT2 mRNA abundance was observed at 2 and 4 dpf. A significant 60-fold reduction in Aanat2 expression was seen just before metamorphosis demonstrating, for the first time in a vertebrate species, that the expression of pineal AANAT and thyroid hormones levels exhibit an inverse pattern during metamorphosis.

Nitric oxide synthase in photoreceptive pineal organs of fish

Photoreceptor cells in the fish pineal gland transduce light–dark information differentially into a neuroendocrine melatonin message; distinguishing features are the presence or absence of endogenous oscillators that drive these rhythms. In the present study, we have analysed the presence and distribution of nitric oxide (NO) synthase in both pineal types by NADPH-diaphorase (NADPHd) histochemistry and determined the effects of NO donors on cGMP formation and melatonin production. NADPHd staining was confined to photoreceptor cells in clock-driven pineal organs of zebrafish and goldfish as evidenced by a codistribution with S-antigen-immunoreactivity (-ir) or cyclic GMP-ir and, in the pineal of the trout, to cells that are S-antigen negative. In the trout pineal, but not in the other species, NADPHd staining was clearly codistributed with growth associated protein-43 (GAP-43) immunoreactivity, an antibody that recognizes developing and regenerating neurons in the fish brain. The presence of a functional NO system in photosensory pineal organs is supported by the fact that NO donors like S-nitroso N-acetylpenicillamine (SNAP) elevate intracellular cGMP levels. However, despite the significant rise in cGMP levels nitric oxide donors did neither affect acute light-dependent melatonin formation in the trout pineal nor the rhythmic production of melatonin in the zebrafish pineal.

Sex differences in energetic costs explain sexual dimorphism in the circadian rhythm modulation of the electrocommunication signal of the gymnotiform fishBrachyhypopomus pinnicaudatus

To understand the evolution of sexually dimorphic communication signals, we must quantify their costs, including their energetic costs, the regulation of these costs, and the difference between the costs for the sexes. Here, we provide the first direct measurements of the relative energy expended on electric signals and show for the focal species Brachyhypopomus pinnicaudatus that males spend a significantly greater proportion of their total energy budget on signal generation (11-22%) compared with females (3%). Both sexes significantly reduce the energy spent on electric signals during daylight hours through circadian modulation of the amplitude, duration and repetition rate of the electric signal, but this effect is more marked in males. Male body condition predicted the energy spent on electric signals (R(2)=0.75). The oxygen consumed by males for signal production closely paralleled the product of the electric signal's waveform area (R(2)=0.99) and the discharge rate (R(2)=0.59), two signal parameters that can be assessed directly by conspecifics. Thus the electric communication signal of males carries the information to reveal their body condition to prospective mates and competing males. Because the electric signal constitutes a significant fraction of the energy budget, energy savings, along with predation avoidance, provides an adaptive basis for the production of circadian rhythms in electric signals.

‘Slicing Through Space’

Providing for the movement of people and things is an inevitable and increasingly important element of urban governance. Urban life in Australian cities has been physically structured around the private motor vehicle, and transport policies have historically focused on the public funding of large-scale public roadways. There is no better example of this approach to transport planning than in Southeast Queensland, where government departments associated with responsibilities for road infrastructure have traditionally wielded enormous power, and have repeatedly attempted to impose large-scale technological solutions for transport problems without regard for their wider impacts on urban life. Despite an emerging awareness of the need to reduce carbon emissions by establishing alternative models of urban mobility, local authorities in Brisbane have recently followed in this well- established tradition by embarking on an ambitious expansion of river crossings and underground road tunnels as a way to increase the capacity of private vehicular flows through the inner city. This article is an exploration of the philosophical framework ‘driving’ these political decisions and their inevitable imposition of new spatio-temporal laws on Brisbane’s inhabitants. Drawing on Henri Lefebvre’s account of the relationship between the production of space and social rhythms, it is argued that these transport planning decisions and the way they govern mobility will reproduce the destructive effects of abstraction on both space and the rhythms of the city.

The planning of the best production route in the assembly system

The goal of this realised analyses is outworking of a methodology enabling the planning of the best production route from the set of alternative production routes. The considered approach is applied to the assembly system described by the set of defined matrices. Rhythmic concurrent production with wide assortment in the considered assembly system is realised. The theoretical roots of the considerations presented in this paper include theory of constraints and critical path scheduling techniques. To workout the three-step methodology is the main achievement of the given paper. The methodology permits to solve the decision problem concerning the selection of the fastest production route from the set of the alternative production routes which are possible for the realisation in the assembly system. The presented methodology can become an integrated part of existing authority software which aids the decision-making process connected with production planning and ensures effective utilisation of production resources. [Received on 11 April 2006; Accepted on 21 February 2007]

自己の内的テンポを考慮した二者間の協調的リズム生成のモデル化

自己の内的テンポを考慮した二者間の協調的リズム生成のモデル化

A β-catenin gradient links the clock and wavefront systems in mouse embryo segmentation

Rhythmic production of vertebral precursors, the somites, causes bilateral columns of embryonic segments to form. This process involves a molecular oscillator--the segmentation clock--whose signal is translated into a spatial, periodic pattern by a complex signalling gradient system within the presomitic mesoderm (PSM). In mouse embryos, Wnt signalling has been implicated in both the clock and gradient mechanisms, but how the Wnt pathway can perform these two functions simultaneously remains unclear. Here, we use a yellow fluorescent protein (YFP)-based, real-time imaging system in mouse embryos to demonstrate that clock oscillations are independent of beta-catenin protein levels. In contrast, we show that the Wnt-signalling gradient is established through a nuclear beta-catenin protein gradient in the posterior PSM. This gradient of nuclear beta-catenin defines the size of the oscillatory field and controls key aspects of PSM maturation and segment formation, emphasizing the central role of Wnt signalling in this process.

Perception and Production of Syncopated Rhythms

THE PROCESSING OF COMPLEX, METRICALLY ambiguous rhythmic patterns, of the sort found in much popular music, remains poorly understood. We investigated listeners' abilities to perceive, process and produce complex, syncopated rhythmic patterns. Rhythmic complexity was varied along a continuum, quantified using an objective metric of syncopation suggested by Longuet-Higgins and Lee. Participants (a) tapped in time to the rhythms, (b) reproduced the same patterns given a steady pulse, and (c) recognized these patterns when replayed both immediately and after a 24-hour delay. Participants tended to reset the phase of their internally generated pulse with highly syncopated rhythms, reinterpreting or "re-hearing" the rhythm as less syncopated. High complexity in rhythmic stimuli can thus force a reorganization of their cognitive representation. Less complex rhythms were more robustly encoded than more complex syncopated rhythms in the delayed memory task. Syncopated rhythms provide a useful tool for future explorations of human rhythmic competence.

Adaptations for life in the Arctic: evidence that melatonin rhythms in reindeer are not driven by a circadian oscillator but remain acutely sensitive to environmental photoperiod

In reindeer Rangifer tarandus, a high latitude species, the rhythmic production of melatonin periodically dissipates under natural photoperiods when, in mid-winter, there is near permanent darkness and again, in summer, when there is permanent light. In spring and autumn, as expected, melatonin production reflects the ambient light:dark (LD) cycle. We investigated the expression of circadian mechanisms on blood levels of melatonin in reindeer. Two experiments were conducted in which animals were transferred from natural photic conditions into continuous darkness for 3 days: (i) in February, when they had been exposed to an LD cycle (11L:13D) and (ii) in July, when they had been exposed to permanent light. In July, plasma levels of melatonin rose abruptly on exposure to darkness but then declined over 24 hr before displaying a second rise and decline over the following 36 hr. In contrast, in February, levels of melatonin rose abruptly but then remained elevated for more than 60 hr in darkness. Melatonin secretion upon exposure to darkness did not conform to a circadian pattern and did not, therefore, support the hypothesis that pineal activity in reindeer is tightly regulated by circadian mechanisms. Instead the secretion of melatonin appeared to be acutely and directly sensitive to ambient lighting. The results are consistent with a model in which Arctic resident animals have adapted to extreme photic conditions by disconnecting the generation of the pineal melatonin signal from their circadian machinery and relying, instead, on its being driven by the LD cycle for just a few weeks annually in spring and autumn.

Meeting Report: The Role of Environmental Lighting and Circadian Disruption in Cancer and Other Diseases

Light, including artificial light, has a range of effects on human physiology and behavior and can therefore alter human physiology when inappropriately timed. One example of potential light-induced disruption is the effect of light on circadian organization, including the production of several hormone rhythms. Changes in light–dark exposure (e.g., by nonday occupation or transmeridian travel) shift the timing of the circadian system such that internal rhythms can become desynchronized from both the external environment and internally with each other, impairing our ability to sleep and wake at the appropriate times and compromising physiologic and metabolic processes. Light can also have direct acute effects on neuroendocrine systems, for example, in suppressing melatonin synthesis or elevating cortisol production that may have untoward long-term consequences. For these reasons, the National Institute of Environmental Health Sciences convened a workshop of a diverse group of scientists to consider how best to conduct research on possible connections between lighting and health. According to the participants in the workshop, there are three broad areas of research effort that need to be addressed. First are the basic biophysical and molecular genetic mechanisms for phototransduction for circadian, neuroendocrine, and neurobehavioral regulation. Second are the possible physiologic consequences of disrupting these circadian regulatory processes such as on hormone production, particularly melatonin, and normal and neoplastic tissue growth dynamics. Third are effects of light-induced physiologic disruption on disease occurrence and prognosis, and how prevention and treatment could be improved by application of this knowledge.