Chloris Gayana Research Articles

Irrigation of rangeland soils with coal seam water - A lysimeter study on soil physico-chemical properties

Groundwater extracted from coal seams may be a resource for irrigation of land in areas with low rainfall, but the effect of this water on soil properties needs to be established. A lysimeter study was conducted using intact soil cores (0.75 m diameter, 1.4 m deep) of four different soil types (Sodic Vertisol, Calcic Solonetz, Haplic Solonetz and Xanthic Lixisol) from southern Queensland, Australia, to study changes in soil physical and chemical properties under accelerated rates of irrigation with coal seam (CS) water (electrical conductivity (ECw) of 3 dS/m, pH of 8.8, and a sodium adsorption ratio (SAR) of 100). Cores were also alternately irrigated with deionised water to simulate rainfall, and either lucerne (Medicago sativa L) or Rhodes grass (Chloris gayana Kunth.) where grown in the lysimeters. The soil surface was treated with stoichiometric rates of elemental sulfur (1.4 t/ha) and gypsum (2.5 t/ha) prior to every 450 mm CS water irrigation to minimise changes in SAR and pH. Three of the soils (Vertisol, both Solonetz) had low leaching fractions (≤ 0.1 %) due to their clay texture and were initially saline in the subsoil (ECse 1.4–4.4 dS/m). Irrigation with CS water resulted in a gradual increase in salt content (EC) and SAR throughout the soil profile, but pH was not increased due to surface-applied elemental sulfur. The Lixisol had a higher hydraulic conductivity and leaching fraction (6.7 %) due to is loamy texture – in this soil, accumulated salts could be leached and no increase in salinity or pH were measured. Despite an increase in SAR for this loamy soil, no structural degradation was observed, and it could be sustainably irrigated with up to 3200 mm CS water (with cumulative irrigation volume of 5400 mm). Hence, leaching fractions rather than soil chemistry are good indicators to identify soils suitable for irrigation with CS water that is saline, alkaline, and sodic.

The essential oil blend Agolin Ruminant L reduces methane production in vitro and in vivo when included in the drinking water of cattle.

Two experiments were conducted to determine the potential for the essential oil blend Agolin Ruminant L (Agolin) to reduce enteric methane (CH4) emissions from beef cattle when delivered via the drinking water. Experiment 1 evaluated aqueous solutions of Agolin (50mg/L) and a non-protein nitrogen and mineral solution (uPRO ORANGE [uPRO]; 1.7mL/L) individually and in combination, where Agolin was added to concentrated uPRO at 3, 4.5, 6% Agolin (w/w) prior to dilution with water at 1.7mL/L, for a total of five treatments. These were incubated for 48h with a medium-quality Rhodes grass (Chloris gayana) hay substrate, with gas production, CH4 concentration in gas, and digestibility measured in vitro. In Exp. 2, Droughtmaster steers (n = 24) were fed a basal diet of Rhodes grass hay and were allocated to one of three water treatments (n = 8/treatment) supplemented with either uPRO (2.27mL uPRO/L water), or one of two inclusion rates of Agolin in combination with uPRO (2.27mL uPRO and 6 µL Agolin/L water or 2.27mL uPRO and 24 µL Agolin/L water) with enteric CH4 emissions, feed and water intake, and live weight gain (LWG) measured over 56 d. In Exp. 1, the inclusion of Agolin in uPRO at 6% w/w resulted in a reduction in CH4 production (15.8%; P = 0.003) and the proportion of CH4 in the gas produced (24.5%; P < 0.001). In Exp. 2, steers consuming the lower quantity of Agolin via drinking water had a 16.4% (P = 0.0027) reduction in CH4 production over the experiment, declining from 140g/d during week 1 to 117g/d in week 8. This inclusion rate of Agolin in the drinking water also resulted in a 25g (17.6%) CH4/d decrease in emissions by steers compared to control steers (P = 0.0205). However, no significant differences in CH4 yield (g CH4/kg dry matter intake), or CH4 intensity (g CH4/kg LWG) by steers was observed between treatments. These results demonstrate that Agolin reduces CH4 emissions when mixed in aqueous solution under in vitro and in vivo conditions, providing a potential method to reduce enteric CH4 emissions from cattle in extensive production systems.

Agronomic performance and nutritive value of Urochloa species, Desho and Rhodes grass grown in sub-humid central Ethiopia

Improved forage grasses with high quality and biomass are a crucial additional feed source for cereal-livestock farming in Ethiopia. This study compared the performance of 4 Urochloa species [U. brizantha (DZF-13379), U. humidicola (DZF-9222), U. decumbens ‘Basilisk’ (DZF-10871) and U. mutica (DZF-483)] with other 2 commonly used grasses, Rhodes grass (Chloris gayana ‘Massaba’) and Desho grass (Pennisetum glaucifolium local variety Kindu kosha), over 3 years during the main rainy season in Bishoftu in a sub-humid area of Ethiopia. The experiment was conducted using a completely randomized block design. There was significant variation among species for agronomic parameters. The species × year interaction was significant for dry matter yield, plant height, and plot cover but not significant for leaf-to-stem ratio. Nutritional value [ash, acid detergent fiber (ADF), crude protein (CP) and in vitro dry matter digestibility (IVDMD)] was significantly different among species with no differences for neutral detergent fiber (NDF) and acid detergent lignin (ADL). All species showed potential as alternative ruminant feeds with U. mutica and U. brizantha the highest yielding in the sub-humid environment.

Is adoption of modern dairy farming technologies interrelated? A case of smallholder dairy farmers in Meru county, Kenya

Attempts have been made to promote the adoption of modern dairy farming technologies (MDFT). However, the adoption of these technologies largely remains underutilized. This study aimed to analyze the determinants of the adoption of MDFTs in Meru County. Using purposive and proportionate sampling techniques from 355 smallholder dairy farmers in Meru County, Kenya, this study analyzed the factors that facilitate or impede the adoption of MDFTs. We use a Multivariate probit (MVP) to evaluate adoption decisions by dairy farm households facing multiple MDFTs. The results reveal a significant correlation among the eight MDFTs suggesting that modern technologies are interrelated. The MVP model results indicate that household income, education level, number of extension contacts, access to credit, farmer group membership, farming experience and livestock monetary value had positive effect on the adoption of MDFTs. Education level and extension contacts had a positive influence on the adoption of fodder establishment and preservation technologies. Farming experience in dairy farming had a positive effect on the adoption of well-structured and clean sleeping areas, and the growth of Rhodes grass. Household income had a positive effect on the growth of Rhodes grass and feed mixture. This work illustrates a need for a policy implication and insight into a need for the county government and private milk processing companies to increase extension frequency to enhance the adoption of MDFTs. Additionally, there is a need to increase access to affordable credit, this should be considered by the government by establishing strengthening a smallholder low-interest and efficient local credit schemes and institutions.

NaCl-induced effects on photosynthesis, ion relations, and growth of Chloris gayana Kunth in the presence of two levels of KCl

Soil salinization is a widespread environmental problem that impacts agriculture. Potassium fertilization is often associated with stress mitigation. Aiming to identify the ability of Rhodes grass (Chloris gayana Kunth) to cope with high salt as well as to investigate the potential of K+ fertilization to alleviate stress symptoms, we investigated the combined effects of NaCl and KCl on photosynthesis, ion distribution, and growth of two Rhodes grass cultivars, Callide and Reclaimer. Plants were grown under different regimes (0, 200, 400, and 600 mM NaCl + 1 or 10 mM KCl). For Reclaimer, 10 mM KCl induced positive effects in photochemistry under 0 and 200 mM NaCl, as illustrated by fluorescence transients OJIP-bands and JIP-test parameters. However, such improvements did not lead to superior biomass accumulation nor net photosynthesis compared to the corresponding treatments under 1 mM KCl, which may not justify KCl application. In Callide 10 mM KCl induced deleterious effects on photochemistry of plants under low NaCl levels. High salinity (600 mM) induced stress-triggered biomass reduction of up to 70% in both cultivars, but all plants remained photosynthetically active. Exposure of both cultivars to NaCl concentrations equal to or higher than 200 mM triggered response mechanisms such as the ability to accumulate inorganic solutes accounting to osmotic potential, stomata closure, and excretion (up to 70%) of the retained Na+ onto the leaf surface irrespective of KCl. Our data reinforce that Rhodes grass is an auspicious forage crop for saline environments and, therefore, in revegetation programs for saline soils pasture in subtropical regions.

Undegradable dietary protein limits growth and carcass yields in crossbred Boer kids fed Desmanthus hay or Rhodes grass (Chloris gayana) hay supplemented with urea and/or cottonseed meal

Context Low-protein tropical forages required protein supplementation, which might affect growth of meat goats. Aims This study was aimed to compare liveweight gain, carcass/non-carcass yields and meat quality in crossbred Boer kids fed isonitrogenous diets varying in undegradable (UDP) and rumen-degradable (RDP) dietary protein sources. Methods Twenty-five female crossbred Boer kids were randomly allocated into the following five dietary treatments: Rhodes grass (Chloris gayana) hay supplemented with urea (U), urea plus cottonseed meal (UCSM), cottonseed meal (CSM), gliricidia (Gliricidia sepium) (Gliri) or Desmanthus (Desmanthus leptophyllus) only (Desman). The diets were formulated to supply 135 and 195 g crude protein/kg DM for the two experiments. Experiment 1 lasted 78 days and the dietary UDP:RDP ratio (g/kg DM) was 35:102 (U), 37:100 (UCSM), 40:97 (CSM), 55:82 (Desman) and 46:91 (Gliri). Experiment 2 lasted 138 days and the dietary UDP:RDP ratio (g/kg DM) was 45:150 (U), 52:143 (UCSM), 58:137 (CSM), 83:112 (Desman) and 71:124 (Gliri) respectively. Data were subjected to one-way ANOVA and general linear model. Key results After 138 days of supplementation, the Desman goats had the highest liveweight gain (83 g/day) and heaviest cold carcass weight (12.1 kg) in Experiment 2. This was followed, in descending order, by CSM (58 g/day and 9.6 kg), UCSM (48 g/day and 7.8 kg), Gliri (41 g/day and 7.6 kg) and U (6 g/day and 6.0 kg). Heavier carcass weight was associated with larger eye-muscle area and fat depth at the 12th rib. Aus-meat Beef Colour Standard scores of 3–4 for the eye muscle of the Desman and CSM goats compared with scores of 1C-2 in the other goats meant that the darker meat in Desman and CSM diets was associated with heavier carcass weight and a more rapid rate of decline in carcass pH. Conclusions Crossbred Boer kids fed high dietary UDP had higher intakes of UDP, RDP, metabolisable protein (MP) and metabolisable energy (ME), even though an isonitrogenous diet was offered. The Desman kids had higher liveweight gain, heavier carcass yield, greater eye-muscle area, thicker fat depth and heavier mass of non-carcass components. Implications The higher liveweight gain and carcass/non-carcass yields for Desman goats than for others was associated with increased DM, UDP, RDP, MP and ME intakes.

Customized Plant Growth Promotion with Soil- and Cultivar-Compatible Microbial Biofertilizers

Organic fertilizers and microbial biofertilizers are now widely recognized to effectively complement traditional mineral fertilizers for plant growth. The present study shows that bio-organic fertilizers can be enhanced by the addition of functional plant-growth-promoting rhizobacteria (PGPR) that provide additional benefits to plants. We hypothesized that not all beneficial soil bacteria are functional in different farm soils and plant varieties; hence, the most effective PGPR that are suitable to each farm’s individual cropping conditions were selected. Five different field soils and their respective crops were tested for compatibility with six microbial biofertilizers (including three new bacterial strains) to supplement a commercially available bio-organic fertilizer. In pot trials with lucerne plants, four out of the six microbial treatments led to significant (p &lt; 0.05) growth promotion benefits (up to 79.8% more leaves and dry weight) compared to mock-treated or bio-organic fertilizer-only-treated control plants. A trial with industrial hemp demonstrated that compatibility with PGPR occurs in a cultivar-specific manner, leading to growth promotion ranging from −3.4% to 68.9%, with each cultivar displaying a preference for a different PGPR. Finally, pot trials with Rhodes grass and two different soils demonstrated high yield increases compared to control plants, with Bacillus amyloliquefaciens 33YE being most effective for one soil and Bacillus velezensis UQ9000N/Pseudomonas lini SMX2 for the other soil. Yield advantages reduced after several cuts of grass, but a repeat biofertilizer treatment at 69 days after the initial treatment restored high yield advantages, with the same PGPR again being most effective. These results demonstrate the importance of customization of microbial inoculants to identify the most compatible PGPR–cultivar–soil interaction. The customization of microbial biofertilizers to soils and plant cultivars, combined with complementary fertilizer applications, can potentially lead to more reliable and more sustainable agricultural practices.

Evaluation of host plant species for a trap cropping strategy in integrated pest management of pineapple mealybug (Dysmicoccus brevipes)

Pineapple mealybugs are responsible for the emergence of pineapple wilt disease and the subsequent transmission of the pineapple disease virus. This study aimed to evaluate the alternate host plants as potential trap crops for pineapple mealybug. A total of nine host plant species, banana, pumpkin, roselle, hibiscus, ground fig, okra, java grass, goose grass and rhodes grass were selected for the study. Thirty adult females of pineapple mealybug were reared on a pumpkin for two months. Three replicates of a plot (area covered with black netting measuring 3 m × 3 m) were set up. Nine host plant species, approximately 2–3 feet in a 16 inch × 16 inch polybag were arranged in a circle around the pumpkin. The effectiveness of the host plant species was determined by calculating the infestation of each plant species for six months (with two-week observations). Our findings revealed that the pineapple mealybug prefers banana with the greatest mean and SD value (621.7±172.7), followed by pumpkin (237.0±45.6) and the lowest one is rhodes grass (2.00±26.5). ANOVA results show that there is a significant difference between host plant species (F-value of 26.87 and a P-value &lt; 0.00). The observations suggest that banana has the potential to be utilized as trap crops for pineapple mealybug, and can be considered in IPM strategy to control pineapple mealybug.

Leveraging Wheat Competition to Manage Seasonal Expansion of Feathertop Rhodes Grass (Chloris virgata)

Utilizing the potential of crops to suppress weeds is an important strategy for sustainable management. Feathertop Rhodes grass (FTR) (Chloris virgata Sw.) is a problematic warm-season weed in Australia that has recently expanded into colder seasons. This study investigated the growth and seed production of FTR at two planting times (May and July) and three wheat planting densities (0, 82, and 164 plants m−2) using a neighborhood design over two years. In both years, the plant height, tiller production, and panicle production of FTR were lower in the presence of wheat compared to when wheat was absent. Surrounding FTR with wheat delayed its pinnacle emergence, varying from 1 to 6 days in the first year and 4 to 21 days in the second year, depending on the planting date and wheat density. During both years and planting times, wheat’s presence caused approximately a 99% decrease in the dry matter and seed production of FTR compared to wheat’s absence. Additionally, the wheat height, an important competitive factor, was higher at both densities in the May planting compared to the July planting. The emergence, establishment, and continuous production of FTR seeds throughout the year indicate that inadequate management could result in the further spread of this weed. Our findings suggest that adjusting the date and density of wheat planting could be a viable strategy for sustainably managing this weed during colder seasons.

Assessing the combination efficiency of some unconventional feed resources with concentrates and Chloris gayana grass in mitigating ruminal methane production in vitro.

In a preliminary in vitro study, leaves of Acacia nilotica, Prosopis juliflora, Cajanus cajan, Leucaena leucocephala and seed kernel of Mangifera indica were identified as potential candidates in mitigating ruminal methane (CH4) production. The objective of the current study was to investigate the combination efficiency of these unconventional feeds with concentrate mix (CM) or Chloris gayana grass in CH4 reduction. Two feed combinations in different proportions were incubated in vitro with buffered rumen fluid at Hohenheim Gas test. In combination 1, C. gayana and CM were included as basal substrates, while in combination 2, A. nilotica, P. juliflora, C. cajan, L. leucocephala or M. indica seed kernel were included as CH4 reducing supplements at different proportions. The CH4 reducing potentials of feed combinations were presented as the ratio of CH4 to net gas production and expressed as percentage (pCH4). The pCH4 for CM and C. gayana was 16.7% and 16.9%, respectively, while it ranged from 3.18% in A. nilotica to 13.1% in C. cajan. The pCH4 was reduced (p < 0.05) from 14.6% to 9.39% when A. nilotica was combined with CM. In combination of L. leucocephala or C. cajan with CM, the pCH4 (p < 0.05) was reduced from 16.5% and 16.6% with the lowest proportion to 15.1% and 15.2% with the highest inclusion rate respectively. The combination of C. gayana with L. leucocephala or C. cajan reduced (p < 0.05) the pCH4 from 16.3% and 16.4% to 15.1% and 14.9% respectively. The pCH4 was reduced (p < 0.05) from 13.4% to 7.60% when A. nilotica was combined with C. gayana. Estimated digestible organic matter (dOM) and metabolizable energy (ME) increased (p < 0.05) with increasing proportions of M. indica seed kernel with CM or C. gayana. In conclusion, the combination of the basal substrates with unconventional supplements resulted in CH4 reduction without affecting the dOM and ME at lower inclusion rates. Animal-based experiments await to validate in vitro findings.

Herbage Yield and Nutritive Value of Selected Grasses in Subhumid Agroecological Environments in Ethiopia

The introduction of improved forage varieties could play a crucial role in meeting the growing demand for livestock and livestock products in Ethiopia. However, different cultivars exhibit varied performance. Therefore, this study aimed to assess the performance of Brachiaria cultivars and other grass cultivars in two subhumid agroecological areas of Ethiopia. Four Brachiaria cultivars (B. brizantha (DZF-13379), B. humidicola (DZF-9222), B. decumbens (DZF-10871), and B. mutica (var. DZF-483), and two local grass cultivars, Rhodes grass (Chloris gayana var. Massaba) and Desho grass (Pennisetum glaucifolium var. Kindu Kosha), were evaluated in Bako and Bishoftu for three years during the rainy season. The experiment was carried out using a completely randomized block design. Results indicated that both cultivars and years had significant (P&lt;0.001) effects on herbage dry matter (DM) yield, crude protein (CP) yield, and plant height, whereas location only affected DM yield. Significant cultivar × year interactions were observed for DM yield, CP yield, and plant height, while cultivar × location interactions had a significant effect on all parameters except for CP yield. Additionally, cultivars significantly (P&lt;0.001) impacted the in vitro dry matter digestibility (IVDMD) as well as the nutritional content (CP, ash, acid detergent lignin (ADL), neutral detergent fiber (NDF), and acid detergent fiber (ADF). Overall, all cultivars demonstrated potential as alternative ruminant feeds, with B. mutica, followed by B. brizantha, showing superior performance in the subhumid agroecological areas of Ethiopia.

Impact of industrial effluent on selected grass species

Industrial wastewater contains a variety of substances such as organic carbon, salts and heavy metals, which along with arsenic (As), cadmium (Cd), lead (Pb) and nickel (Ni) are known to be highly toxic even in very low concentrations. This study was carried out to test the impact of industrial effluent from the Industrial Estate Islamabad, the capital city of Pakistan, on four local grass species of the Pothohar region, i.e., Panicum maximum (Mombasa grass), Cenchrus ciliaris (Dhaman grass), Cynodon dactylon (Khabbal grass) and Chloris gayana (Rhodes grass). These grasses were grown in tap water (T0), industrial effluent (T1), and in industrial effluent-tap water ratio (50:50; T2). The concentrations of As, Cd, Pb, and Ni accumulated by these grasses were appraised with inductively coupled plasma optical emission spectrometry (ICP-OES). All four grasses differed significantly in terms of their accumulation of different types of metal elements in the shoot and root organs. The analysis of industrial effluent, and root and shoot biomass samples of all four grasses did not have high amounts of heavy metals with reference to the levels of the metals documented by WHO. It is generally recommended that the effluents from the Industrial Estate Islamabad can be used with some caution for growing forage grasses including the four species tested in this study.

Endopolyploidy in the Leaf Cells of Rhodes Grass (Chloris gayana Kunth)

Endopolyploidy in the Leaf Cells of Rhodes Grass (<i>Chloris gayana</i> Kunth)

Long-term evaluation of pasture production, seasonality, and variability: An application of the DairyMod pasture model for three tropical species

Adoption of improved pastures coupled with intensified management provide quality pastures in adequate quantities and thus improve livestock productivity. While pasture modelling is imperative for exploring the performance of newer pastures, models are little used for long-term simulations of multiple tropical pastures (genotype), under varying soil, climate (environment) and pasture production systems (management). We applied the DairyMod, a biophysical model to simulate the long-term pasture production of Brachiaria ruziziensis x B. decumbens x B. brizantha ‘Brachiaria Mulato II’ (BM), Megathyrsus maximus ‘Gatton Panic’ (GP), and Chloris gayana ‘Rhodes grass cv. Reclaimer’ (RR) across major dairying regions of Sri Lanka under different management scenarios and characterize the long-term pasture growth, seasonality and spatial variability, and possible implications for dairying in Sri Lanka. Simulations of three pasture species were carried out for 16 locations (8 dry (DZ), 5 intermediate (IZ), and 3 wet zone (WZ)) over 30 years (1980–2010). Three pasture management scenarios simulated were; 1) potential pasture production system under non-limiting N and irrigation (Yp) 2) rainfed pasture production system under non-limiting N fertilizer (Yw), and 3) rainfed pasture production system under current nitrogen (N) fertilizer rate (Ya). Statistical techniques were used to identify the long-term growth rates, variability, and trends in pasture production. The long-term pasture production varied greatly among climate, species, and management scenarios. Overall, the Ya showed a seasonal cycle following the rainfall pattern, with a reduction in growth rates in dry seasons (May–September). Pasture growth rates were greater in GP at Ya, and BM at Yw and Yp while RR showed the lowest growth rate at all times. Variability of pasture growth was high in DZ (May–September) and RR has the lowest growth variability. The Yw increased the growth rate (doubled) while the Yp substantially increased (nearly tripled) the growth rate and growth pattern producing less variable pastures. Simulated growth rates suggest that GP in low-input and BM in high-input farming areas would be more suitable. Our study suggested that the BM, GP, and RR are edaphic-climatologically fit for major dairying regions in Sri Lanka and the appropriate fertilizer and irrigation management can greatly increase the herbage accumulation and availability of year-round pastures. While this study offers valuable insights, the species-specific growth pattern, growth variability, yield potential under different managements and the possible implications for herbage quality need to be sensibly considered when selecting the appropriate species.

Utilization of the Neighborhood Design to Evaluate Suitable Pasture Crops and Their Density for Navua Sedge (Cyperus aromaticus) Management

Navua sedge (Cyperus aromaticus), a perennial plant native to Africa, poses a significant weed concern due to its capacity for seed and rhizome fragment dissemination. Infestations can diminish pasture carrying capacity, displacing desirable species. Despite the burgeoning interest in integrated weed management strategies, information regarding the efficacy of competitive interactions with other pasture species for Navua sedge management remains limited. A pot trial investigated the competitive abilities of 14 diverse broadleaf and grass pasture species. The results indicated a range of the reduction in Navua sedge dry biomass from 6% to 98% across these species. Subsequently, three broadleaf species—burgundy bean (Macroptilium bracteatum), cowpea (Vigna unguiculata), and lablab (Lablab purpureus), and three grass species—Gatton panic (Megathyrsus maximus), Rhodes grass (Chloris gayana), and signal grass (Urochloa decumbens) were chosen for a follow-up pot trial based on their superior dry biomass performance. These six species were planted at three varying densities (44, 88, and 176 plants/m2) surrounding a Navua sedge plant. Among the grass pasture species, Gatton panic and Rhodes grass exhibited high competitiveness, resulting in a minimum decrease of 86% and 99%, respectively, in Navua sedge dry biomass. Regarding the broadleaf species, lablab displayed the highest competitiveness, causing a minimum decrease of 99% in Navua sedge dry biomass. This study highlights the increasing efficacy of crop competition in suppressing weed growth and seed production, with the most significant suppression observed at a density of 176 plants/m2.

Nonessential amino acids in tropical ruminant feed: Investigating grass and legume forages of Indonesia.

This study aimed to examine the concentration of nonessential amino acids (NEAAs) in ruminant feed in tropical areas, with a focus on forage grasses and legumes in Indonesia. A total of 11 grasses (Chrysopogon zizanioides, Brachiaria brizantha, Brachiaria humidicola, Paspalum dilatatum, Paspalum atatum, Chloris gayana, Pennisetum polystachion, Panicum maximum, Cenchrus biflorus, Andropogon canaliculatus, and Digotaria decumbens) and six legumes (Arachis hypogaea, Pueararia Javanica, Centrosema pubescens, Clitoria ternatea, and Arachis pintoi) were analyzed for NEAA content using high-performance liquid chromatography (HPLC). Based on the results of this research, it was found that almost all NEAA content in forage was less than 3% (Serine (Ser), Alanine (Ala), Glycine (Gly), Tyrosine (Tyr), Proline (Pro), Cysteine (Cys), and Asparagine (Asn), except for glutamic (Glu) acid and arginine (Arg) in some legumes. The Glu content in grass ranges from 0.76% to 2.61%, and the Arg content ranges from 0.92% to 2.09%. These two NEAAs were most abundant in grasses and legumes, with concentrations of 5.10% to 6.27% and 3.10% to 5.53%, respectively. Our study concluded that Glu and Arg were the most abundant NEAAs in tropical forages in Indonesia, with legumes having a higher concentration of NEAAs compared to grasses. Among the legumes, A. hypogaea had the highest NEAA content (23.40%), while among the forages, C. zizanioides had the highest NEAA content (12.37%). However, it was observed that neither legumes nor grasses could fulfill the metabolizable TNEAAs requirements for gaining cattle (250 kg of empty body weight gain), unlike commercial concentrates, which were found to meet the requirements, especially for Arg, Glu, and thyronine. The provision of concentrate is necessary to supplement forage to meet the NEAA needs for cattle.

Supplementation of crossbred boer goats with cottonseed meal and/or urea enhances estimated metabolizable protein intake, crude protein digestibility and nitrogen retention

This study aimed to determine feed intake, apparent digestibility, and nitrogen retention of crossbred Boer bucks supplemented with urea or cottonseed meal (CSM)when fed tropical Rhodes (Chloris gayana) grass hay (RGH) as a basal diet. Twelve eight-month-old bucks (23.9±1.1 kg live weight) were allocated to one of the four dietary treatments in a randomised block design. The treatments were 1) maize supplement (Control); or maize plus 2) urea supplement (M+U), 3) urea and CSM (M+UCSM); and (4) CSM (M+CSM). All bucks were held in individual metabolism crates for 21 days (11 days of adaptation and 10 days collection period) with ad libitum access to RGH and fresh drinking water in addition to the supplements. A one-way ANOVA and Duncan’s multiple range tests were used to test for differences between means at a significance level of P &lt; 0.05. Results indicated higher feed intake, apparent digestibility of crude protein and digestible nutrient intake in goats supplemented with M+CSM and M+UCSM, but not M+U alone. Supplementation with M+CSM increased nitrogen retention by 300%. Supplementation with M+UCSM and M+CSM increased the total feed intake of Boer goats on tropical grass hay by 13 and 20%, respectively. Supplementation with M+U, M+UCSM, and M+CSM improved the apparent digestibility of crude protein by 9.7, 15.7 and 15.1%, respectively. It can be concluded that total feed intake and nutrient digestibility was increased on growing Boer goats fed RGH as a basal diet supplemented with true protein from CSM or a mixture of true protein and rumen degradable protein from urea.

The effect of supplementing different levels of chicory and yeast to growing dairy goats fed on Rhodes grass basal diet on feed intake and weight gain

The effect of supplementing different levels of chicory and yeast to growing dairy goats fed on Rhodes grass basal diet on feed intake and weight gain

In-vitro digestibility and methane gas emission of indigenous and introduced grasses in the rangeland ecosystems of south eastern Kenya

Various grass species with high biomass yield and low moisture demand have been introduced in the rangelands of Kenya to realize increased ruminant productivity that could not be achieved with the low quality of the indigenous grasses. However, this intervention ignores the different methane emission of the indigenous and introduced grasses, a necessary consideration for realizing increased productivity while minimizing greenhouse gas emissions. This study determined in-vitro digestibility and methane emission of three indigenous grasses: Eragrostis superba (E. superba), Cenchrus ciliaris (C. ciliaris), Enteropogon macrostachyus (E. macrostachyus) and two introduced grasses (two varieties of Chloris gayana; Boma rhodes and Extozi rhodes. Samples of these five grasses (whole plant above ground) were collected from established pasture plots in South Eastern rangelands of Kenya. The grass samples were collected at bloom stage using one-meter square quadrats for proximate analysis and determination of neutral detergent fiber (NDF), acid detergent lignin (ADL) and acid detergent fiber (ADF) using AOAC (1990) methods. On average, relative to the indigenous grasses, the introduced grasses were higher in crude protein (74.05 g Kg-1 dry matter (DM) vs. 52.11 g Kg-1 DM), organic matter digestibility 62.7% vs 53.6%) and in NDF (712.7 g Kg-1 DM vs. 708.0 g Kg-1 DM), metabolizable energy (16.35 vs 12.90 MJ/kg DM), methane emission (25.61 ml vs 15.93 ml) but with lower in-vitro-dry matter digestibility 54.24% vs 58.12%. Methane production positively correlated with crude protein, NDF, metabolizable energy, ADF and in-vitro organic matter digestibility. Hence, utilizing the introduced grasses to boost cattle production would achieve increased productivity but a point of concern are the higher methane emissions, not to mention the ecosystem change caused by the introduction of new species, which should affect the sustainability of the rangeland ecosystem.

Elemental sulfur effects on salt leaching, plant growth, nutrient uptake, and microbial diversity in an arid saline soil

The amendment of salt-affected soils with elemental sulfur (S0) has been recognized for its potential to effectively reduce soil salinity and pH, thereby enhancing soil physicochemical properties, promoting crop growth, and improving yields. Despite these known benefits, the widespread adoption of S0 for managing saline soils, particularly in arid calcareous regions, remains limited. Consequently, this study aimed to evaluate the impact of S0 on salt leaching, soil pH, nutrient uptake, plant growth, microbial diversity, and community structure under alkaline saline soil conditions. The study includes three main experiments: a preliminary column experiment without plants, a field trial with Rhodes grass (Chloris gayana), and a greenhouse pot experiment with wheat (Triticum spp.). Our findings demonstrated that S0 incorporation into the soil exerted a positive influence, resulting in increased salt removal, reduced soil pH, and improved plant growth. Specifically, soil amendment with 750 kgS0ha−1 led to a substantial salt removal, exceeding double in the column experiment, approximately 91.3 % in the field trial, and about 34.1 % in the greenhouse pot experiment compared to the control. S0 amendment also significantly lowered soil and leachate pH in both field and greenhouse trials, with reductions of 3.3 % and 6.3 %, and 8.1 % and 4.4 %, respectively, relative to the control. Calcium and phosphorus uptake by Rhodes grass increased significantly by 75 % and 14 %, respectively, compared with the control. Soil organic matter content significantly increased from 0.6 % to 1.5 % compared to that of the control. This overall enhancement in soil conditions resulted in a considerable increase in Rhodes grass and wheat yields by 13 % and 59 %, respectively. While the prokaryotic diversity (16S V4 rRNA sequencing) in the Rhodes grass field trial was not significantly affected by S0 amendments after two months, the microbial community composition showed remarkable differences between the S0-amended and control samples. Eighty-three unique taxa were exclusively found in the S0-amended samples. In conclusion, our findings suggest that the amendment of soil with S0 is a promising strategy for the sustainable management of calcareous salt-affected soils in arid regions.