NaCl-induced effects on photosynthesis, ion relations, and growth of Chloris gayana Kunth in the presence of two levels of KCl

Abstract

Soil salinization is a widespread environmental problem that impacts agriculture. Potassium fertilization is often associated with stress mitigation. Aiming to identify the ability of Rhodes grass (Chloris gayana Kunth) to cope with high salt as well as to investigate the potential of K+ fertilization to alleviate stress symptoms, we investigated the combined effects of NaCl and KCl on photosynthesis, ion distribution, and growth of two Rhodes grass cultivars, Callide and Reclaimer. Plants were grown under different regimes (0, 200, 400, and 600 mM NaCl + 1 or 10 mM KCl). For Reclaimer, 10 mM KCl induced positive effects in photochemistry under 0 and 200 mM NaCl, as illustrated by fluorescence transients OJIP-bands and JIP-test parameters. However, such improvements did not lead to superior biomass accumulation nor net photosynthesis compared to the corresponding treatments under 1 mM KCl, which may not justify KCl application. In Callide 10 mM KCl induced deleterious effects on photochemistry of plants under low NaCl levels. High salinity (600 mM) induced stress-triggered biomass reduction of up to 70% in both cultivars, but all plants remained photosynthetically active. Exposure of both cultivars to NaCl concentrations equal to or higher than 200 mM triggered response mechanisms such as the ability to accumulate inorganic solutes accounting to osmotic potential, stomata closure, and excretion (up to 70%) of the retained Na+ onto the leaf surface irrespective of KCl. Our data reinforce that Rhodes grass is an auspicious forage crop for saline environments and, therefore, in revegetation programs for saline soils pasture in subtropical regions.