Rheumatoid Factor In Patients Research Articles

Assessment of Liver Enzymes Activity in Patients with Rheumatoid Arthritis in Nineveh province

Aim of the study: to determine the effect of Rheumatoid Arthritis(RA) on liver enzymes level. Methods: 75 subjects were enrolled in this study (40 patients with Rheumatoid arthritis (RA) and 35 healthy subjects as control group), at Mosul city, Northern of Iraq, aged 20-70 years. The biochemical parameters measured were some of liver function tests which include measurement of the liver enzymes activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and gamma glutamyl transferase (GGT). Results: There was a significant increase in liver enzymes activity in patients when compared to control. There was also significant increase in serum AST level at age group 40-50 years when compared to other age group in patients, in addition, there was a significant increase in serum ALP in female patients when compared to male patients. There was non-significant change in liver enzymes activity when compared according to residence, according to family history, and according to rheumatoid factor in patients. Conclusion: The results of the present study indicated no deterioration in liver functions in patients with rheumatoid arthritis regarding serum enzymes (aspartate transaminase, alanin transaminase, gamma glutamyl transferase and alkaline phosphatase), although subclinical alteration in liver functions could be expected in those patients, so that measurement of other early markers of liver dysfunction is occasionally recommended.

Elevated Nesfatin-1 Level in Synovium and Synovial Fluid is Associated with Pro-Inflammatory Cytokines in Patients with Rheumatoid Arthritis.

BackgroundAdipocytokines have been proven to be involved in the progression of autoimmune diseases, including rheumatoid arthritis (RA). Nesfatin-1, a newly discovered adipokine, has recently been reported to possess potent anti-inflammatory, antiapoptotic, and antioxidative properties. However, its role in RA has not yet been reported. Therefore, this study aimed to determine nesfatin-1 levels in the synovium and synovial fluid (SF) of patients with RA and examine their correlation with clinical manifestations and proinflammatory cytokine levels.MethodsSynovium and SF samples were collected from patients with RA and non-RA patients during joint surgery. Immunohistochemistry was used to measure nesfatin-1 protein expression in the synovium. Enzyme-linked immunosorbent assay was used to measure nesfatin-1, interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) levels in the synovium and SF. Pearson correlation analysis was used to evaluate the correlations between nesfatin-1 levels, RA clinical features, and proinflammatory cytokines. The diagnostic value of synovium nesfatin-1 for RA was assessed using receiver operating characteristic (ROC) curve analysis.ResultsThe results showed that nesfatin-1, IL-1β, and TNF-α levels in the synovium were significantly higher in patients with RA than in controls, with age and body mass index as covariates. Moreover, the results of Pearson correlation analysis showed that nesfatin-1 levels were positively correlated with IL-1β and TNF-α levels in the synovium of patients with RA. Furthermore, there was a positive relationship between synovium nesfatin-1 levels and rheumatoid factor in patients with RA. Additionally, the results of the ROC curve analysis revealed an area under the curve of 0.733 with 77.5% sensitivity and 60.0% specificity for synovium nesfatin-1 in discriminating patients with RA from controls.ConclusionThese findings suggest that increased nesfatin-1 levels in the synovium may be associated with proinflammatory cytokines and RA severity.

Effects of nonsurgical periodontal therapy in patients with rheumatoid arthritis: a prospective before and after study.

Background: periodontal therapy has been suggested to have systemic effects. However, studies of periodontal therapy in rheumatoid arthritis patients have produced controversial results.Aim: To compare the effects of nonsurgical periodontal therapy on biochemical markers of rheumatoid arthritis and periodontal parameters in patients with and without rheumatoid arthritis.Methods: a prospective before-and-after study was conducted that included 21 participants without and 29 participants with rheumatoid arthritis. Periodontal parameters, Porphyromonas gingivalis detection, C-reactive protein, rheumatoid factor and anti-citrullinated protein antibodies were measured at baseline and three months after nonsurgical periodontal therapy and the changes were statistically assessed.Results: In general, both groups presented statistically significant improvement in periodontal parameters (p<0.05). There was an increase in the counts of P. gingivalis in both groups at three months. In addition, there was a reduction in levels of anti-citrullinated protein antibodies and rheumatoid factor in participants with rheumatoid arthritis. In contrast, C-reactive protein levels increased in both groups but were higher in the rheumatoid arthritis group. Periodontal parameters in rheumatoid arthritis participants under disease-modifying antirheumatic drugs presented a slightly higher improvement (p <0.05).Conclusions: Nonsurgical periodontal therapy has similar improvements in periodontal parameters in patients with and without rheumatoid arthritis. In addition, nonsurgical periodontal therapy may benefit serum levels of anti-citrullinated protein antibodies and rheumatoid factors in patients with rheumatoid arthritis. NCT04658615.

Association of serum CXCL12 levels with arthropathy in patients with systemic sclerosis.

Systemic sclerosis (SSc) is an autoimmune connective tissue disease, in which extensive fibrotic change and vasculopathy affect the skin and various internal organs. It also involves the joints, causing stiffness, arthralgia, and arthritis. Although arthropathy is commonly observed in SSc, its underlying mechanism remains unknown. CXCL12, also known as stromal cell derived factor 1, is associated with inflammation, mesenchymal cell recruitment, angiogenesis, and collagen production, and is implicated in the development of various joint diseases. To assess the potential contribution of CXCL12 to SSc development, we investigated the clinical association of serum CXCL12 levels in patients with SSc. We conducted a cross-sectional analysis of 68 patients with SSc and 20 healthy controls recruited in a single center over 9years. Serum CXCL12 levels were measured by enzyme-linked immunosorbent assay. Serum CXCL12 levels were significantly higher in patients with SSc than in healthy controls (median 1554.0pg/mL, 25th-75th centiles 1313.0-1914.0pg/mL vs 967.4pg/mL, 608.8-1271.0pg/mL, P< 0.001). Patients with SSc with elevated CXCL12 levels had significantly more cases of arthropathy than those with normal CXCL12 levels (85.7% vs 25.0%, P=0.01). Furthermore, patients with SSc with elevated CXCL12 levels showed an increased trend in the prevalence of limited range of motion of the finger joints compared with those with normal CXCL12 levels (60.0% vs 18.6%, P=0 .07). Moreover, serum CXCL12 levels were significantly correlated with the titers of rheumatoid factor in patients with SSc (r=.41, P=0.001). Elevated serum CXCL12 levels may be related to the development of SSc arthropathy.

Analysis of serum rheumatoid factors in patients with rheumatoid arthritis in Han, Tibetan and Hui nationalities in Qinghai

ObjectiveThe aim of this study was to compare the sensitivities, specificities and correlations of serum rheumatoid factors across different races in RA patients. MethodsSerum rheumatoid factors were tested in 150 subjects who were divided into 3 groups: group 1 including 25 Han RA patients (Han-RA) and 25 healthy Han control groups, group 2 including 25 Tibetan RA patients (T-RA) and 25 the healthy Tibetan control group and group 3 including 25 Hui-RA patients (Hui-RA) and 25 Hui healthy controls. ResultsThere were significant differences in ESR, A-CCP, CRP, RF, SAA, SFe, IL-4, IL-6, IL-10 and IL-17 between RA patients and the corresponding control subjects in all 3 groups (P < 0.01 for ESR, A-CCP, CRP, RF, IL-4, IL-6, IL-10 and IL-17, P < 0.05 for SAA and SFe). In Tibetan RA, the levels of A-CCP were significantly lower than the Han-RA and Hui-RA. SAA of T-RA was significantly lower than the Han-RA (P < 0.05). ConclusionESR, CRP, RF, A-CCP, SAA, SFe and IL serum rheumatoid factors were useful biomarkers for RA detection in all three races.

Relationship between bone mineral density and anti-citrullinated protein antibody and rheumatoid factor in patients with rheumatoid arthritis.

ObjectiveRheumatoid arthritis (RA) is one of the causes of osteoporosis, and it leads to systemic bone loss. The anti-citrullinated protein antibody (ACPA) and rheumatoid factor (RF) are associated with local and systemic low bone mineral density and osteoclast-mediated bone resorption independently of inflammation in patients with RA. In this article, we aimed to evaluate the relationship between the ACPA, RF, and systemic bone mineral density in patients with RA.MethodsNinety-three patients (6 male, 87 female) with RA were included in the study. The disease activity score 28-erythrocyte sedimentation rate and titers of RF, ACPA, and bone mineral density of the total hip, femoral neck, and lumbar areas were evaluated. The independent samples t-test, Mann-Whitney U-test, Spearman’s correlation, and multivariable regression analysis were used for the statistical analysis.ResultsThe RF and ACPA were positive in 40.9% and 48.4% of patients with RA, respectively. Disease activity was negatively correlated with the T- and Z-scores. The T- and Z-scores were lower in the seropositive group than in the seronegative group. The ACPA was negatively correlated with the T- and Z-scores of the femoral neck. There was a significant difference for the Z-score of the femoral neck in patients with ACPA and RF-positive patients compared to seronegative patients with RA.ConclusionA low bone mineral density, especially in the femoral neck, is associated with the presence of ACPA and RF. It would be a more appropriate approach to carefully monitor osteoporosis in seropositive RA patients.

76-Year-Old Man With New Bilateral Shoulder Pain

76-Year-Old Man With New Bilateral Shoulder Pain

The Relationship among Interlukin-10 ,Interlukin-6 with anti-cyclic citrullinated peptide antibody, C-reactive protein ,Rheumatoid factor in patients with Rheumatoid Arthritis

Rheumatoid arthritis (RA), the most severe disease affect the joint and also the most common systemic autoimmune disease, affecting approximately 1% of the adult population .We investigate the serum levels of IL-10 and IL-6 in patients with Rheumatoid arthritis. About forty patients with Rheumatoid arthritis (30 female and 10 male) with ages ranged between (20-70) years were taken from (Al-Hussein Medical City/Kerbala).Control group consisted of 20 healthy people were free from signs and symptoms of arthritis were matched in age and gender with patients, and had no history for any arthritis problem .IL-10 (IL-10-EASIA Kit, DIAsource) (IL-6 ELISA kit ,immunotech),(Anti CC-P ELISA kit ,Medizym/Germany ) ,(RF ELISA Kit Euroimmun/Germany) were measured using the enzyme-linked immunosorbent assay (ELISA) method and CRP Vital kit using quantitative turbidimetry method . t-test , ANOVA and Pearson correlation were used to analyze results by using SPSS version 22. P-value ≤ 0.05 was considered significant. IL10,IL6,Anti-CCP,CRP and RF were increased significantly (p&lt; 0.05) in patients compared with control group, So increasing of IL10,IL6,Anti-CCP,CRP and RF values at mild stage (41.4±1.3),sever stage(54.8±0.8),sever stage(952±114), server stage(49.3±1),sever stage (181.3±11) respectively increased significantly value (p&lt; 0.05) among the stages, The results revealed no significant value (p&gt;0.05) among the age groups and according the gender groups, While there were highly significant correlation (p&lt; 0.05) found among studded parameters .

Anti Cyclic Citrullinated Peptide and Rheumatoid Factor in Patients with HCV Arthropathy

Background: HCV related arthritis is not uncommon and both diagnosis and treatment is challenging and the role of anti cyclic citullinated peptide (Anti CCP) in the diagnosis of HCV arthropathy is unknown. Materials and methods: A case control Prospective study conducted on 60 patients known to have chronic Hepatitis c infection with arthropathy (Study group) and 20 healthy subjects as control group. Results: The most frequent affected joint in the study was PIPs 32 (53.3%) followed by MCPs joint in 26 (43.3%) while the least frequent joint was the elbow that was affected in only one patient (1.7%). Ten patients (16.6%) had picture of arthritis while 50 (83.4%) had arthralgia. Serum RF in our study it was positive in 16 (26.6%) with low titre with mean 77.01 ± 11.05 (nine of these patients had arthralagia with mean 68.09 ± 13.44, and seven patients had arthritis with mean 80.77 ± 11.45).The prevelance of total cases with positive cryoglobulinemia had estimate about 26 (43.33%). The Anti ccp among HCV arthropathy and we found that about (8 patients 13.33%) have low positive titer for Anti ccp with mean 15.77 ± 5.05. Conclusion: Anti cyclic citrunated peptide can be detected among patients with HCV arthropathy with positive low titer.

Comparing antibody responses to &lt;i&gt;Onchocerca volvulus&lt;/i&gt; and non-parasite antigens in placebo-controlled and ivermectin-treated onchocerciasis patients

Serum antibodies to parasite-specific and non-parasite antigens were evaluated using enzyme-linked immunosorbent assay (ELISA). Out of the 470 sera collected, 409 were from residents of an onchocerciasis hyper-endemic area, 55 non-endemic and 6 European normal sera served as control. The patients’ age, sex, skin microfilaria densities, dermal and ocular clinical manifestations (colour of optic disc) have been well characterised. The study population had participated in a placebocontrolled (n=191) trial of ivermectin (Mectizan®) treatment (n=218). The parasite antigens are phosphate buffered saline crude extract of adult worms of Onchocerca volvulus, a recombinant antigen (Ov1.9) and a monoclonal antibody purified antigen (Cam 1). The non-parasite antigens are deoxycholate citrate extract of optic nerve (nerve-DOC) and commercially available IgA, IgM and IgG were used to assay for rheumatoid factor (Rh-F) auto-antibodies. Generally, antibodies to parasite antigens in onchocerciasis patients were remarkably higher than control group (p&lt;0.05) using exact F-test. There was no significant difference (p&gt;0.05) in antibodies to nerve-DOC and Rh-F in patients compared to control. Antibodies increased with increasing skin snip microfilaria load from 0.69±0.28 with 0mf/mg (n=54) as against 0.80±0.26 for those with 4-20mf/mg. Observed slight negative correlation in IgG antibody levels and severity of disc colour with mean OD values of 0.26±0.22 in those graded as having no optic nerve disease (OND) (disc 1, n=86) and 0.17±0.19 for those with severe changes (disc 3, n=49) was not statistically significant (P&gt;0.05). An age dependent significant decrease (P&lt;0.05) in antibodies were observed with 0.64±0.34 for 15-30yr old (n=48) compared to 0.48±0.35 for those 50yr (n=50) for PBS with a similar trend for IgG to Ov1.9 and Cam1. In conclusion, serum parasite-specific and non-parasite antibodies may not be responsible for the pathology of optic nerve disease. Onchocerciasis patients were apparently not at higher risk of developing rheumatoid arthritis than the control.Keywords: Onchocerciasis; Antibodies; Antigens; Immune responses; Ivermectin.

THE ROLE OF PLATELETS IN FORMATION OF NEUTROPHIL EXTRACELLULAR TRAPS IN PATIENTS WITH IMMUNOCOMPLEX PATHOLOGY

The article presents the results of the assessment of the role of platelets in formation of extracellular traps by neutrophils. We have detected the ability of platelets to oppress the formation of extracellular traps by neutrophils in vitro cultures in patients with rheumatoid arthritis (RA) (n = 42) and systemic lupus erythematosus (SLE) (n = 24), but not in patients with hemorrhagic vasculitis (GW) (n = 15). The study has revealed the interrelation of NETosis and rheumatoid factor in patients with RA and SLE, as well as NET formation and number of platelets in patients with GW.

A Missense Variant of TNFAIP3 is Associated with the Presence of Rheumatoid Factor in Patients with HCV-Related Lymphoma

A Missense Variant of TNFAIP3 is Associated with the Presence of Rheumatoid Factor in Patients with HCV-Related Lymphoma

Anti-citrullinated protein antibodies suppress let-7a expression in monocytes from patients with rheumatoid arthritis and facilitate the inflammatory responses in rheumatoid arthritis

We hypothesized that anti-citrullinated protein antibodies (ACPAs) could affect the expression of miRNAs in monocytes and contribute to the inflammatory responses in rheumatoid arthritis (RA). The expression profiles of 270 human miRNAs, co-cultured with ACPAs or human immunoglobulin G (IgG), were analyzed using real-time polymerase chain reaction. Ten miRNAs exhibited differential expression in U937 cells after co-cultured with ACPAs compared with human IgG. The expression levels of these miRNAs were investigated in monocytes from 21 ACPA-positive RA patients and 13 controls. Among these miRNAs, the expression levels of let-7a was decreased in monocytes from ACPA-positive RA patients. The expression levels of let-7a showed a negative correlation with positivity of rheumatoid factor in patients sampled. We found that transfection of U937 cells with let-7a mimic suppressed K-Ras protein expression. In the ACPA-mediated signaling pathway, transfection of U937 cells with let-7a mimic suppressed the ACPA-enhanced phosphorylation of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase 1/2 (ERK1/2), and the expression and secretion of interleukin (IL)-1β. In conclusion, ACPA-mediated decreased let-7a expression in monocytes from ACPA-positive RA patients. Decreased let-7a expression was associated with the positivity of RF in ACPA-positive RA patients. The decreased expression of let-7a could facilitate the inflammatory pathway via enhanced ACPA-mediated phosphorylation of ERK1/2 and JNK and increased expression of IL-1β through an increase in the expression of Ras proteins.

Anti-cyclic citrullinated peptide and rheumatoid factor in patients with chronic hepatitis B and hepatitis B carriers.

Rheumatoid factor (RF) positivity that may occur in a number of patients with hepatitis B (HBV) infection poses challenges in terms of differential diagnosis with rheumatoid arthritis (RA). On the other hand, antibodies to cyclic citrullinated peptide (anti-CCP) may prove to be an important marker for differential diagnosis of the two conditions. This study aimed to assess anti-CCP and RF positivity among patients with hepatitis B and rheumatoid arthritis. Anti-CCP and RF seropositivity was assessed in 61 patients with HBV infection (32 patients with chronic hepatitis, 29 patients with inactive HBV carrier status) and 40 patients with RA as the control group. RF positivity was found in 18.7% and 34.4% of the patients with chronic hepatitis B and inactive HBV carrier status, respectively. On the other hand, only one patient with chronic HBV had low positive anti-CCP. RF was positive in 24 (60%) and anti-CCP was positive in 26 (65%) patients among the 40 patients with RA. Anti-CCP may be helpful in the differential diagnosis between RA and chronic HBV infection or inactive HBV carrier status.

Relationship between Periodontitis and Rheumatoid Arthritis: Review of the Literature.

Periodontitis (PD) and rheumatoid arthritis (RA) are immunoinflammatory diseases where leukocyte infiltration and inflammatory mediators induce alveolar bone loss, synovitis, and joint destruction, respectively. Thus, we reviewed the relationship between both diseases considering epidemiological aspects, mechanical periodontal treatment, inflammatory mediators, oral microbiota, and antibodies, using the keywords “periodontitis” and “rheumatoid arthritis” in PubMed database between January 2012 and March 2015, resulting in 162 articles. After critical reading based on titles and abstracts and following the inclusion and exclusion criteria, 26 articles were included. In the articles, women over 40 years old, smokers and nonsmokers, mainly constituted the analyzed groups. Eight studies broached the epidemiological relationship with PD and RA. Four trials demonstrated that the periodontal treatment influenced the severity of RA and periodontal clinical parameters. Nine studies were related with bacteria influence in the pathogenesis of RA and the presence of citrullinated proteins, autoantibodies, or rheumatoid factor in patients with PD and RA. Five studies investigated the presence of mediators of inflammation in PD and RA. In summary, the majority of the articles have confirmed that there is a correlation between PD and RA, since both disorders have characteristics in common and result from an imbalance in the immunoinflammatory response.

Expression of the Inherently Autoreactive Idiotope 9G4 on Autoantibodies to Citrullinated Peptides and on Rheumatoid Factors in Patients with Early and Established Rheumatoid Arthritis

BackgroundThe pre-symptomatic stage of Rheumatoid arthritis (RA) is associated with pro-inflammatory cytokines and autoantibodies. High levels and epitope spread by Rheumatoid factors (RhF) and autoantibodies to citrullinated proteins signify progression towards disease expression. In established RA, the persistence of high autoantibody levels reflects production by both long-lived plasma cells and short-lived plasmablasts. Neither the relative contributions to pathogenesis by autoantibodies from either source, nor the factors responsible for deciding the fate of autoantigen specific ‘parent’ B-cells, is understood. Phenotypic markers identifying subsets of autoreactive B-cells are therefore of interest in understanding the origin and perpetuation of the autoimmune response in RA. One such phenotypic marker is the rat monoclonal antibody, 9G4, which recognises an idiotope on immunoglobuins derived from the inherently autoreactive VH-gene, VH4-34. We therefore investigated whether the 9G4 idiotope was expressed on autoantibodies in patients with RA.Methodology/Principal FindingsSera from 19 patients with established RA and those with <1year history of untreated polyarthritis either resolving into RA (n = 42) or non-RA diagnosis (n = 31) were included. Autoantibodies to cyclic citrullinated peptides (CCP), RhF and co-expression of the 9G4 idiotope were measured by ELISA. 9G4 recognised a population of anti-CCP antibodies in the majority of sera from patients with established disease and also in samples from patients with early disaese. 9G4+RhF levels were generally lower and not associated with positivity for, or levels of 9G4+CCP.Conclusions/SignificanceThe persistence of 9G4+ immunoglobulins, of any isotype, in serum is rare. We describe here the novel finding of 9G4 expression on anti-CCP antibodies in patients from the earliest symptoms of RA through to established disease. Our results suggest that 9G4 expression on anti-CCP autoantibodies was not due to polyclonal expansion of VH4-34-encoded immunoglobulins. These studies may therefore provide a new focus for investigation into the evolution of the autoimmune response in RA patients.

AB0227 Extra Articuar Manifestations of Rheumatoid Arthritis - A Hospital Based Study in Albania

BackgroundThe frequency of Extra-articular manifestations of Rheumatoid arthritis differs in various populations. They have been mostly found in patients with positive Rheumatoid Factor and positive ANA.ObjectivesTo identify the presence and...

A Comparative Assessment of the Diagnostic Value of Anti-cyclic Citrullinated Peptide Antibodies and Rheumatoid Factor in Rheumatoid Arthritis

Rheumatoid arthritis is a systemic, chronic inflammatory autoimmune disease affecting many tissues but principally attacking the joints. Auto antibodies such as rheumatoid factor and anti-cyclic Citrullinated peptide antibodies have important diagnostic value. This cross-sectional analytical study was performed at a single medical institution in central Iran (Shahid Sadoughi Hospital, Yazd, Iran) in order to compare the diagnostic value of anti-cyclic citrullinated peptide antibodies and rheumatoid factor in patients with rheumatoid arthritis. Serum levels of anti-cyclic citrullinated peptide antibodies were determined by enzyme-linked immunosorbent assay, and levels of rheumatoid factor were determined by turbidimetry on a latex-enhanced agglutination assay in 266 patients with rheumatoid arthritis and 134 patients with non-rheumatoid arthritis rheumatic diseases. Among the 266 patients with rheumatoid arthritis, 188 patients (70.7%) were tested positive for anti-cyclic citrullinated peptide antibodies, and 123 patients (46.2%) were tested positive for rheumatoid factor. The sensitivity, specificity, positive predictive value and negative predictive value of anti-cyclic citrullinated peptide antibodies for diagnosing rheumatoid arthritis were 70.76%, 85.07%, 90%, and 59% respectively. Those for rheumatoid factor were 46.26%, 90.29%, 90%, and 45% respectively. The anti-cyclic Citrullinated peptide antibodies measurement is a useful test for diagnosing rheumatoid arthritis. Due to its high positive predictive value and negative predictive value, it is an important diagnostic test and gives accurate diagnosis of the disease. However, this does not mean that anti-cyclic citrullinated peptide antibodies can replace rheumatoid factor in the diagnosis of rheumatoid arthritis, because not all rheumatoid arthritis patients have anti-cyclic Citrullinated peptide antibodies. The two tests therefore appear to be complementary.

AB1235 Clinical relevance of anti-citrullinated protein antibody for the detection of rheumatoid arthritis in hemodialysis patients

BackgroundHemodialysis (HD) patients are considered to be at increased risk for developing rheumatoid arthritis (RA), as a result of immunologic abnormalities caused by this treatment1). Anti-cyclic citrullinated peptide (anti-CCP) antibody...

AB0312 Effect of tocilizumab on anti-cyclic citrullinated peptide antibodies and rheumatoid factor in patients with rheumatoid arthritis

BackgroundRheumatoid arthritis (RA) is a systemic autoimmune disease characterized by synovial inflammation that eventually results in joint destruction and functional damages. Rheumatoid factor (RF) and anti-cyclic citrullinated peptide antibodies (anti-CCP)...