Abstract A sensitive, precise and simple spectrophotometric method has been proposed and validated for the determination of oseltamivir (OSL) in pharmaceutical formulations. The proposed method is based on the reaction between the OSL and 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-CL) at alkaline medium (pH 10) to form deep brown adduct, exhibiting maximum absorption (λmax) at 464 nm. Under optimized reaction condition, the method was linear in the concentration range 2-10 μg mL-1. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.84 μg mL-1, 2.66 μg mL-1, respectively. The method was applied successfully to the determination of OSL in pharmaceutical formulation. A proposal of the reaction pathway has been postulated. The method is useful for routine analysis of OSL in quality control laboratories.