RGB Color Space Research Articles

PUPMCR: An R Package for Image-Based Identification of Color Based on Rayner’s (1970) Terminology and Known Fungal Pigments

Abstract Fungi are eukaryotic organisms grouped based on different traits of their morphology. In 1970, R. W. Rayner published A Mycological Colour Chart to provide a standardized system for identifying color in fungi. While its terminologies have contributed a standard way of color matching for taxonomic diagnoses, this method employing the personal color perception of the observer does not guarantee accuracy. Considering the diversity of fungi, visual color matching is expected to be challenging without a standard assisting instrument. In this study, the R package PUPMCR is developed to approximate the color name and associated pigments of fungal species based on the pixel coordinates of its uploaded image. This software utilizes CIELAB and RGB color spaces as well as Euclidean and Chi-square distance metric systems. The package is tested and validated using 300 fungal images as a dataset for conducting interrater reliability tests. Results showed the highest agreement for parameters utilizing the RGB color space (Cohen’s kappa values: 0.655 ± 0.013 for RGB and Euclidean; 0.658 ± 0.004 for RGB and Chi-square), attributed to its computational efficiency, which facilitates more uniform binning and universally scaled distance metrics. The produced color-identifying tool is also available as a Shiny web application (https://pupmcr.shinyapps.io/PUPMCR/) to allow better accessibility for users on the World Wide Web. The development of PUPMCR not only benefits a variety of users from its free accessibility but also provides a more reliable color identification system in the field of mycology.

Wide Color Gamut and Multi‐Band Color Filtration With MIM Structure Plasmonic Filters

AbstractColor filters are extensively utilized in various applications, including photovoltaic devices, printing, liquid crystal displays, image sensors, digital photography, projection systems, and other optical instruments. In this paper, an F‐P cavity structure based on an metal‐insulator‐metal (Ag‐ZnS‐Ag) structure is designed. By selecting specific materials and tuning structural parameters, strong coupling effects are formed through the interaction between the Fabry‐Pérot (FP) resonance in the intermediate dielectric cavity and the LSP resonance on the metal surface. This structure can generate a narrow‐band resonance peak within the visible light spectrum. By tuning the thickness of the dielectric cavity, color filtration across the 380–700 nm range can be dynamically obtained, enabling continuous multi‐band filtering within visible light. Within the visible light range, this structure, functioning as a filter, can achieve a transmission efficiency of up to 56% and a FWHM of 10–24 nm. Additionally, this structure can be used to design wide‐color gamut plasma filters for constructing RGB color spaces. When the ZnS thickness is 55 nm, it corresponds to the blue channel of the color space; at 75 nm, it corresponds to the green channel; and at 95 nm, it corresponds to the red channel. This color space can cover 100% of the sRGB color gamut and 99.946% of the Adobe RGB color gamut. In summary, this structure provides a new approach for designing simple, photolithography‐free plasma filters that enable multi‐band color filtration and exhibit a wide color gamut.

Machine vision system combined with multiple regression for damage and quality detection of bananas during storage

The study used a machine vision system and applied image process analysis to assess the bruise parameters of the damage zone and external quality attributes of 18 treatments generated from bruised (30 cm and 60 cm drop heights) and non-bruised (control) ‘Fard’ and ‘Somali’ banana cultivars stored at 5, 13, and 22 °C for 21 d Experiments include digital image analysis of fractal dimension (FD), grayscale (Igray), bruise susceptibility (BS), color, surface area (AS), and some other physical attributes like weight loss %, peel thickness reduction %, and firmness. Multiple linear regression analysis was programmed to determine the effect of the four predictors (storage time, temperature, impact level, and cultivars) on the dependent variables (bruise intensity and quality parameters). It was demonstrated that the fractal dimension of the bruised area increased as impact damage and storage duration increased, particularly in high impact (60 cm) ‘Fard’ banana fruit at 5 and 22 °C, respectively. Digital image analysis was effectively used to determine the surface area of both cultivars. The model explains 83 % of the variability in digital image surface area compared to 70 % for manually measured surface area. The predicted versus measured L*a*b* color spaces showed a higher determination of coefficient (R2 > 0.5300) compared to a lower determination of coefficient (R2<0.3900) for RGB color spaces. The L*a*b* color spaces recorded a strong correlation with all digitally processed parameters, peel thickness, firmness, and weight loss %, which had greater reliability than RGB color space to predict subsequent bruise damage and storage quality change in bananas. Using image analysis by machine vision system aligning with multiple linear regression has been approved to be efficient for external quality and bruise intensity determination in banana cultivars.

Colour sorting ROS-based robot evaluation under different lights and camera angles

Automated colour sorting, aided by mobile robots, is widely prevalent in the current manufacturing industry. Obstacles, such as fluctuating light conditions and camera angles, frequently hinder this procedure. Creating a colour sorting robot is a complex and time-consuming task, especially due to the vulnerability of the RGB colour space to detection errors in extreme brightness or darkness. In response to these concerns, we introduce a mobile robot that operates on the robot operating system (ROS) platform and incorporates OpenCV. This robot employs the hue, saturation, and value (HSV) colour space model for its image processing capabilities in recognising the colours and Welzl’s algorithm for the ball’s diameter estimation. The robot’s performance was assessed across various luminous fluxes and camera tilt angles. It demonstrated exceptional performance at 64 lm and a tilt angle of 40 degrees, achieving an average accuracy of 87.5% for detecting the colour of the ball, and 81.25% for determining its location based on colour. For the ball’s diameter estimation, it was found that the best estimation was received at 64 lm and 30 degrees, with both 96.32%.

Hue-preserving lightness and saturation modification in RGB color space for protanopia and deuteranopia

Colors are not seen in the same way among all people. For example, to dichromats such as protanope and deuteranope, red and green appear similar ochre-like colors. Therefore, it is difficult for dichromats to distinguish such color combinations. The purpose of this study is to propose a color conversion method that produces images that are easy for dichromats to discriminate colors, without significantly changing the impression of the original image for both dichromats and trichromats. The proposed method modifies the lightness of pixels to take into account colors that are difficult for dichromats to discriminate, thus enabling color discrimination. Furthermore, by also modifying the saturation of the pixels, the image quality degradation caused by overemphasis, which occurs when only modifying the lightness, is suppressed. To demonstrate the effectiveness of the proposed method, experiments using various images are conducted. From the resultant images, it can be confirmed that the proposed method achieves the color conversion that serves its purpose. The main results of quantitative evaluation are as follows. (1) In the index evaluating the degree of contrast improvement for colors which are difficult for dichromats to discriminate, the average value of the proposed method is about 0.84, and indicates that the proposed method is comparable to the conventional methods. (2) In the index represented by the ratio of the number of pixels clipped with white or black, the average of the proposed method is 4.38×10−4 and indicates that there are almost no clipped white and black pixels.

Efektivitas Penggunaan Ruang Warna HSV untuk Klasifikasi Daging Sapi Segar dan Busuk dalam Industri Pangan

Beef is a source of animal protein which is very important in the human diet. The quality of beef determines the nutritional value and taste of the processed meat product. However, the quality of beef can decrease over time, especially if it is not stored properly. Therefore, identifying the condition of beef is crucial to ensure that consumers get safe and quality products. The use of the HSV (Hue, Saturation, Value) color space for beef classification is an interesting method to research. The HSV color space is closer to human perception of color compared to the RGB color space, making it more effective for image analysis in the context of visual quality assessment of meat. In this study, researchers used HSV space extraction to classify fresh beef and bad beef. This research aims to develop a method for classifying fresh, medium and rotten beef using the HSV color space. This research produces accurate extraction results with appropriate classification of fresh and bad beef.

Research on the veneer defect image enhancement algorithm based on AMEF-AGC+

Aiming at the veneer defect image acquisition process is prone to the problems of blurred edges, inconspicuous contrast and distortion, which cannot show the defects clearly.To improve image analyzability and clarity, a veneer defect image enhancement method based on AMEF-AGC is proposed herein. First, a veneer defect image is subjected to Gamma correction to obtain multiple underexposed image sequences for which Gaussian and Laplacian pyramids are constructed to determine the weights of the multiple exposure sequence group images.Multiscale fusion is then performed based on these weights. Second, the fused image is converted into the HSV color space, where contrast and brightness enhancements are performed for the luminance component, and then converted back to the RGB color space to obtain an enhanced veneer defect image. Solid wood panels selected herein were pine, poplar and birch with defects including live knots, dead knots, and cracks.Compared with those obtained using several algorithms including AMEF, AGC, improved AGC and GC, this algorithm achieved 6.93% and 5.4% improvements in PSNR and SSIM metrics, respectively.Results demonstrated that the proposed method effectively enhanced veneer defect images with blurred and distorted edges, improved image clarity and visual quality, and made defect parts and details of the image clearer

Research on Grading Method for Jin Guan Apples based on Machine Vision

As the demand for apples increases, so do the expectations for apple quality. To address the time-consuming and labor-intensive nature of apple grading, this paper focuses on the Jin-Guan apple variety and applies machine vision techniques to extract three key features: maximum transverse diameter, shape index, and surface color. A backpropagation neural network (BP neural network) is employed to achieve accurate grading of the apples. First, by analyzing the relationships between the RGB color space components in the original images, the apple images are extracted using the method of comparing the R channel value to the B channel value. Gaussian filtering is then applied to remove image noise. Second, the complete apple contour is extracted using an improved Sobel operator method. The maximum transverse diameter and shape index of the apple are calculated based on the pixel coordinates along the contour, and the apple's surface color is determined using a nearest-neighbor classifier. Finally, a BP neural network model is established and trained to perform the grading. A total of 160 apples were used for training, and 140 for testing. The results show that the grading accuracy reached 92.14%, and the grading efficiency was approximately 1.7 times that of a skilled human grader. This grading method provides a technical reference for apple grading processes.

The Srgb Color Space Based Density Analysis for Brain Tumor Segmentation

One of the important areas of study in medical image processing is early disease detection and diagnosis. A subfield of medical image processing includes the brain tumour segmentation procedure. Medical professionals have an efficient means of diagnosing illnesses because to computer vision and machine learning technology. The Srgb based density analysis is used in this study to isolate the area of brain tumours in MRI images. To differentiate the tumour area from the surrounding area, the intensity values of the input are normalised using the Gaussian filter and the Srgb colour space. In brain MRI samples, the adaptive threshold approach aids in locating potential tumour space. By calculating region parameters like area and density function, the actual space occupied by brain tumours is recovered. Ultimately, by applying morphological functions and removing potential false positives, the accurate tumour space is identified. Recall, accuracy, and F-measure are a few of the performance indicators that are used to evaluate how effective the suggested strategy.

Fusion of visible and fluorescence imaging through deep neural network for color value prediction of pelletized red peppers.

A non-destructive method for determining the color value of pelletized red peppers is crucial for pepper processing factories. This study aimed to investigate the potentiality of visible and fluorescence images for the determination of color value of pelletized red pepper. The imaging problem, caused by the cylindrical shape and irregular cross-sectional features of the pelletized red peppers, was reduced through the extraction of an approximate plane region. To integrate the information in the visible and fluorescence images, a baseline convolutional neural network (CNN) architecture was designed and low level, middle level, and high level fusion models (denoted as LL-CNN, ML-CNN, and HL-CNN, respectively) were developed upon the baseline CNN. The effects of input image size and color space were examined. According to the training result, CNN fusion models were developed using visible image in L*a*b* color space and fluorescence image in RGB color space using 56×56 input image size. Among the three types of CNN fusion models, the HL-CNN obtained the best performance, resulting in Rv 2 of 0.828 and RMSEV of 0.351. This study suggests that the fusion of visible and fluorescence image through CNN is a practical approach to save testing time and replace traditional destructive method. The low cost and compact structure of the imaging systems can maintain the commercial appeal of pepper industry.

Enhancing Underwater Images through Multi-Frequency Detail Optimization and Adaptive Color Correction

This paper presents a novel underwater image enhancement method addressing the challenges of low contrast, color distortion, and detail loss prevalent in underwater photography. Unlike existing methods that may introduce color bias or blur during enhancement, our approach leverages a two-pronged strategy. First, an Efficient Fusion Edge Detection (EFED) module preserves crucial edge information, ensuring detail clarity even in challenging turbidity and illumination conditions. Second, a Multi-scale Color Parallel Frequency-division Attention (MCPFA) module integrates multi-color space data with edge information. This module dynamically weights features based on their frequency domain positions, prioritizing high-frequency details and areas affected by light attenuation. Our method further incorporates a dual multi-color space structural loss function, optimizing the performance of the network across RGB, Lab, and HSV color spaces. This approach enhances structural alignment and minimizes color distortion, edge artifacts, and detail loss often observed in existing techniques. Comprehensive quantitative and qualitative evaluations using both full-reference and no-reference image quality metrics demonstrate that our proposed method effectively suppresses scattering noise, corrects color deviations, and significantly enhances image details. In terms of objective evaluation metrics, our method achieves the best performance in the test dataset of EUVP with a PSNR of 23.45, SSIM of 0.821, and UIQM of 3.211, indicating that it outperforms state-of-the-art methods in improving image quality.

Performance Analysis of a Color-Code-Based Optical Camera Communication System

In this study, we present a visible light communication (VLC) system that analyzes the performance of an optical camera communication (OCC) system, utilizing a mobile phone camera as the receiver and a computer monitor as the transmitter. By creating color channels in the form of a 4 × 4 matrix within a frame, we determine the parameters that affect the successful transmission of data packets. Factors such as the brightness or darkness of the test room, the light color of the lamp in the illuminated environment, the effects of daylight when the monitor is positioned in front of a window, and issues related to dead pixels and light bleed originating from the monitor’s production process have been considered to ensure accurate data transmission. In this context, we utilized the PyCharm, Pydroid, Python, Tkinter, and OpenCV platforms for programming the transmitter and receiver units. Through the application of image processing techniques, we mitigated the effects of daylight on communication performance, thereby proposing a superior system compared to standard VLC systems that incorporate photodiodes. Additionally, considering objectives such as the maximum number of channels and the maximum distance, we regulated the sizes of the channels, the distances between the channels, and the number of channels. The NumPy library, compatible with Python–Tkinter, was employed to determine the color levels and dimensions of the channels. We investigate the effects of RGB and HSV color spaces on the data transmission rate and communication distance. Furthermore, the impact of the distance between color channels on color detection performance is discussed in detail.

Image enhancement for dichromatic vision by geometric approach in RGB color space

AbstractThe goal of this study is to process digital images to facilitate color discrimination in dichromatic vision without changing their appearances in trichromatic vision as much as possible. Therefore, we propose a simple and effective image enhancement method using the geometric properties of the RGB color space. Concretely, a novel image representation that clarifies the relationship between dichromatic and trichromatic visions in the RGB color space, and an image enhancement method based on this representation are presented. The effectiveness of the proposed method is verified by experiments using several digital images.

A-327 Statistical Technique-based Image Analysis for Digital Reading of Immunochemistry Cassette

Abstract Background Covid-19 transformed healthcare and how we think about clinical diagnostics. People prefer at home testing options that enable them to focus on staying healthy at their own comfort and privacy. Fast and accurate lateral flow assays or rapid diagnostic test (RDT) meets that need, however ensuring high test specificity and accuracy are of utmost importance. Visual readout of RDTs is subjective and time consuming. With the current technological advancement, medical devices are smarter and can be used for automated readout. The core of such devices are the algorithms that process the information despite several limitations and unknown variations affecting input signals. Many such algorithms are developed and are used for similar applications. Here, a statistical model for any optical device that is designed to automatically read an RDT with one or more test lines is described. Methods The described LFA algorithm is a innovative way to analyze images acquired with Complementary Metal-Oxide-Semiconductor (CMOS) based optical system with low Signal-to-noise ratio (SNR), giving a semi quantitative result. This also takes care of variations in illumination sources with relatively reduced computation, by converting the images to a 1 Dimensional (1D) signal. Algorithm workflow: Image pre-processing: Affine transformations such as rotation, translation, etc. (depends on the inherent setup) are applied on the acquired images as a pre-processing step. Pattern matching techniques using Gaussian templates are applied to determine the window region of the LFA and precisely identify the test and control line regions. Image formatting: The sample region of the cassette is converted from RGB color space to the weighted gray (Gw) for digital interpretation of the primary biomarker present. Characterization of the Gw channel enables test peak detection with enhanced resolution. This helps in early call out of results and improved accuracy, thereby reducing chances of erroneous results. A second order Butterworth low pass filter is applied to reduce system noise and enhance SNR. The images are converted to 1D signal by averaging for mathematical read out. Mathematical interpretation: Maximum peak height (max) and minimum peak depth (min) of Gw channel values in the test line region (t-peak) are extracted from the 1D signal. The mathematical representation of the test line is calculated as: Test Peak Signal = {max(t-peak) - min(t-peak)} / Alpha; Normalization Factor, Alpha = (sum of all Gw channel values for any clear non-reactive part of the test device) / (number of Gw channel values) Results TPS values for various biomarkers from statistically significant replicates were computed at Low, Medium, and High levels. Two sample T-tests were performed, and respective p values are distinctly different as p-value&amp;lt;0.05. For a concentration level of Low to medium, p-value is 0.044 and for Medium - High p-value is 0.004. Conclusions This algorithm can run on any off-the-shelf available RDT coupled with a CMOS based optical system. The proposed algorithm can differentiate between low, medium, and high concentrations with high confidence. High throughput semiquantitative digital readouts are robust, accurate and are preferred for diagnostic test result interpretation.

Salient Region Guided Colour Image Restoration using Deep Learning with Adaptive Compressive Sensing

In this research, a unique colour image reconstruction method based on Compressive Sensing is presented, aimed at enhancing efficiency and quality. The proposed method incorporates deep learning techniques, adaptive measurement rate setting, and salient region detection. The process begins with the collection of an input RGB images, followed by pre-processing to convert them to the Y+ colour space., including optimized Faster R-CNN (OFRCNN), are employed to detect and segment salient regions in the Y-channel. An adaptive measurement rate setting using Deep Q-Network (DQN) dynamically adjusts measurement rates based on input data characteristics. To optimize the Y-channel compression, a Dual attention-based CS Network (CSNet) model is applied. Moreover, adaptive weighted reconstruction is introduced, utilizing salient region masks and a hybrid the Walrus Optimization Algorithm (WaOA) and mountain gazelle optimizer (MGO), called Hybrid Walrus with Mountain Gazelle Optimizer (HWMGO). Finally, the restored Y-channel is combined with the reconstructed U and V channels to reconstruct full-colour in the RGB colour space. Experimental evaluations on a diverse dataset demonstrate the effectiveness of the proposed algorithm in accurately reconstructing high-quality colour images from CS data. This research contributes to the advancement of colour image reconstruction techniques, offering a comprehensive and efficient framework for various applications.

Comparison Classification Of Tomatoes Ripeness Based On RGB, HSV And CMYK Colors Based On Correlation Coefficient

This article discusses the classification of tomato fruit maturity based on color space. Several studies have been conducted to measure maturity levels using RGB and HSV color spaces. In this article, researchers classify the ripeness of tomatoes using the CMYK color space, which researchers have never done before. Next, the classification results of the CMYK color space are compared with the RGB and HSV color spaces. The CMYK color space is a secondary color commonly seen by the human eye. CMYK colors are colors produced from a combination of RGB colors. Comparison of classification results based on CMYK, RGB, and HSV color spaces was carried out using the correlation coefficient and mean square error (MSE). The correlation coefficient is a method that is often used to measure the similarity between 2 images, where the closer to 0 the correlation value, the better

Multi-color space features and data regression neural network-based method for molten salt temperature detection in rare earth electrolytic processes

To address the online monitoring of molten salt temperature in the rare earth electrolysis process, this paper proposes a rare earth molten salt temperature detection model (MSTDM) based on multi-color space features and data regression neural networks. The model initially preprocesses the collected molten salt temperature images using techniques such as the Gaussian Mixture Model (GMM) and median filtering, followed by color space transformation. Subsequently, 19 hue features used for temperature quantification are extracted, including the channel responses of RGB, HSV, Lab, and Gray color spaces. Finally, a novel data regression neural network (CNN-BiLSTM-Attention) is employed to learn the long-term dependencies between molten salt hue features and temperature, predicting future molten salt temperatures. Experimental results indicate that using the GMM algorithm to filter out flame interference, introducing novel temperature prediction features, and constructing the CNN-BiLSTM-Attention network all enhance the performance of MSTDM in molten salt temperature prediction tasks. The model achieves a mean absolute error of 3.16 °C in predicting molten salt temperatures at rare earth smelting sites. This research not only provides an effective solution for online monitoring of molten salt temperatures in the rare earth electrolysis process but also offers a reliable reference for temperature monitoring in other fields.

Tone mapping algorithm based on BL-Hilbert-L 2 decomposition model for HDR image

Abstract Tone mapping algorithms are mainly used to produce standard dynamic range (SDR) images from high dynamic range (HDR) images. To address the issues of halo artifacts and over-enhancement in current tone mapping algorithms based on layer decomposition model, we propose a tone mapping algorithm based on the BL-Hilbert-L2 decomposition model. In our proposed algorithm, we first convert HDR image from RGB space to HSV space. Subsequently, utilizing the BL-Hilbert-L2 decomposition model, we decompose the V channel of the HDR image into cartoon, texture and high-frequency detail components. Next, we separately compress the cartoon component and stretch the texture and high-frequency detail components. The processed cartoon, texture and high-frequency detail components are then recombined to form a new V channel. Finally, by converting the H and S channels of the HDR image, along with the newly formed V channel, back into the RGB color space, we obtain a tone mapped SDR image. Experimental results demonstrate that our proposed algorithm can effectively eliminate the halo artifacts and avoid the issue of over-enhancement while retaining the edge details.

An underwater image enhancement method based on multi-scale layer decomposition and fusion

High-quality underwater images can intuitively reflect the most realistic underwater conditions, guiding for underwater environmental monitoring and resource exploration strongly. But when factors like light absorption affect underwater optical imaging, it has been found that poor visibility and blurred texture details occur in acquired images, posing challenges for the identification and detection of underwater targets. To obtain natural images, an enhancement algorithm is proposed based on multi-scale layer decomposition and fusion. The algorithm employs different strategies to recover image attenuation information from both local and global perspectives, generating two complementary preprocessed fusion inputs. For fusion input 1, operations are conducted in the RGB color space. Initially, the mean proportion of each color channel is used to identify the attenuated color channel. Then, a local compensation strategy is adaptively applied to restore the pixel intensity of the attenuated color channel. Finally, a statistical color correction method is used to eliminate color cast in the image. Fusion input 2 involves two processing stages. In the Lab color space, the algorithm uses the grayscale information to reduce the deviation in the mean values of channels a and b globally. The local mean information of the component L enhances detail textures. In the RGB color space, linear stretching is applied to correct color deviations. To fuse the structural features of two complementary preprocessed inputs and avoid interference between signals from different layers, the color channels of fused input image are first decomposed into muti-scale structural layers based on structural priors. Then, the image enhancement is achieved through layer-by-layer fusion of the corresponding color channels of the two inputs. By testing and analyzing with the, it was found that the proposed method can improve the clarity of attenuated images in various underwater scenarios of UIEBD and RUIE datasets effectively, enhancing image detail and texture richness, increases contrast, and achieving natural and comfortable visual quality. Compared with the quantitative metrics of 14 other algorithms, the proposed algorithm shows an average score improvement of 10.14, 90.48, and 2.06, respectively, in metrics AG (average gradient), EI (edge intensity), and NIQE (natural image quality evaluator). In the RUIE dataset, it shows an average score improvement of 10.21, 94.76, and 1.86, respectively.

Feature Fusion GAN Based Virtual Staining on Plant Microscopy Images.

Virtual staining of microscopy specimens using GAN-based methods could resolve critical concerns of manual staining process as displayed in recent studies on histopathology images. However, most of these works use basic-GAN framework ignoring microscopy image characteristics and their performance were evaluated based on structural and error statistics (SSIM and PSNR) between synthetic and ground-truth without considering any color space although virtual staining deals with color transformation. Besides, major aspects of staining, like color, contrast, focus, image-realness etc. were totally ignored. However, modifications of GAN architecture for virtual staining might be suitable by incorporating microscopy image features. Further, its successful implementation need to be examined by considering various aspects of staining process. Therefore, we designed, a new feature-fusion-GAN for virtual staining followed by performance assessment by framing a state-of-the-art multi-evaluation framework that includes numerous metrics in -qualitative (based on histogram-correlation of color and brightness); quantitative (SSIM and PSNR); focus aptitude (Brenner metrics and Spectral-Moments); and influence on perception (semantic perceptual influence score). For, experimental validation cell boundaries were highlighted by two different staining reagents, Safranin-O and Toluidine-Blue-O on plant microscopy images of potato tuber. We evaluated virtually stained image quality w.r.t ground-truth in RGB and YCbCr color spaces based on defined metrics and results are found very consistent. Further, impact of feature fusion has been demonstrated. Collectively, this study could be a baseline towards guiding architectural upgrading of deep pipelines for virtual staining of diverse microscopy modalities followed by future benchmark methodology or protocols.