Reversible Properties Research Articles

Repartitioning the Hamiltonian in many-body second-order Brillouin-Wigner perturbation theory: Uncovering new size-consistent models.

Second-order Møller-Plesset perturbation theory is well-known as a computationally inexpensive approach to the electron correlation problem that is size-consistent with a size-consistent reference but fails to be regular. On the other hand, the less well-known many-body version of Brillouin-Wigner perturbation theory has the reverse properties: it is regular but fails to be size-consistent when used with the standard MP partitioning. Consequently, its widespread use remains limited. In this work, we analyze the ways in which it is possible to use alternative non-MP partitions of the Hamiltonian to yield variants of BW2 that are size-consistent as well as regular. We show that there is a vast space of such BW2 theories and also show that it is possible to define a repartitioned BW2 theory from the ground state density alone, which regenerates the exact correlation energy. We also provide a general recipe for deriving regular, size-consistent, and size-extensive partitions from physically meaningful components, and we apply the result to small model systems. The scope of these results appears to further set the stage for a revival of BW2 in quantum chemistry.

Physicochemical Characterization of Hyaluronic Acid-Methylcellulose Semi-Gels for Mitochondria Transplantation.

Traumatic spinal cord injury (SCI) presents a significant medical challenge due to its intricate nature and treatment complexities. SCI can cause physical impairments by affecting neural and motor functions as well as initiating a series of pathophysiological events exacerbating the initial trauma. Leakage from ruptured neurons and vessels disrupt ionic balance and induces excitotoxicity, leading to progressive cellular degeneration. Introducing mitochondria to the SCI lesion has shown potential in attenuating secondary injury. Mitochondria transplantation improves cellular bioenergetics and reduces concentration of reactive oxygen species achieving homeostasis and neuroprotection. Nonetheless, keeping mitochondria viable outside cell environment for a time longer than a few minutes proves to be challenging. Additionally, localized delivery to the injury site has also been limited by other factors including flow rate of cerebrospinal fluid that washes away mobilized organelle from the compromised tissue site. Previously we showed that using hyaluronic acid-methylcellulose semi-gels (HAMC) as a biocompatible, erodible thermogelling delivery vehicle helped to overcome some of these challenges. HAMC allows for controlled release at and around the injury site, utilizing the reverse thermogelling property of MC. Sustained release of mitochondria at slower rate can increase their uptake in spinal tissue. To better optimize the semi-gel delivery of mitochondria requires a more complete understanding of the physicochemical properties of the HAMC semi-gels. We have used ultraviolet-visible spectroscopy to measure optical density of HAMC semi-gels for different HA to MC ratios and examine the temperature dependent gelation properties above their low critical solution temperature (LCST). The viscosity and degree of crystallinity of the resulting HAMC semi-gels were also assessed. Semi-gel erosion and mitochondrial release over time were studied using a fluorescence microplate reader. Lastly, seahorse assay was used to study released mitochondria respiration and viability after incubation in HAMC semi-gel.

Visible to Near‐Infrared Luminescence and Reversible Photochromism in Mn2+/Nd3+ Co‐Doped Double Perovskite for Versatile Applications

AbstractMultifunctional optical materials with temperature sensing and anti‐counterfeiting properties play an essential role in the commercial applications. Herein, Mn2+/Nd3+ co‐doped Cs2AgInCl6 (CAIC) lead‐free double perovskites (DPs) is synthesized through a hydrothermal method, which exhibits visible to near‐infrared (NIR) luminescence and reversible photochromic properties from yellowish to dark purple. The mechanism investigations on the photoluminescence and photochromic phenomena reveal that the incorporation of Mn2+ ions not only acts as an intermediary in the energy transfer process from the host exciton to Nd3+ ions, but also plays a pivotal role in the reversible photochromic response. The temperature‐dependent luminescence, attributed to the contrasting thermal responses of Mn2+ and Nd3+ ions, enables precise temperature sensing via the fluorescence intensity ratio method. In addition, the integration of visible red emission, NIR luminescence, and photochromic properties in a single CAIC: Mn2+/Nd3+ DPs, offers a multilevel anti‐counterfeiting strategy. The multifunctional CAIC: Mn2+/Nd3+ DPs would open up new avenues for advanced optical applications, particularly in the realms of temperature sensing and security anti‐counterfeiting.

Axially Chiral TADF Imidazolium Salts for Circularly Polarized Light‐Emitting Electrochemical Cells

A pair of axially chiral thermally activated delayed fluorescent (TADF) enantiomers, R‐TCBN‐ImEtPF6 and S‐TCBN‐ImEtPF6, with intrinsic ionic characteristics were efficiently synthesized by introducing imidazolium hexafluorophosphate to chiral TADF unit. The TADF imidazolium salts exhibited a high photoluminescence quantum yield (PLQY) of up to 92%, a small singlet–triplet energy gap (∆EST) of 0.04 eV, as well as reversible redox properties. Furthermore, the enantiomers showed distinct mirror‐image CD and CPL activities with glum values of ‐3.7×10‐3 and +3.4×10‐3. Notably, by doping the axial TADF imidazolium salts into achiral TADF sensitizer, sandwich‐structured light‐emitting electrochemical cells (LECs) without the addition of ionic liquids (ILs) or ionic transition‐metal compounds (iTMCs) were fabricated. When driven at 50 A m‐2, the LECs displayed an EQE of up to 5.2% and strong circularly polarized electroluminescence (CPEL) with gEL values of +3.3×10‐3 and ‐3.0×10‐3. This represents the first CP‐LEC based on TADF materials and offers a promising strategy for the development of high‐performance CPEL devices.

Axially Chiral TADF Imidazolium Salts for Circularly Polarized Light-Emitting Electrochemical Cells.

A pair of axially chiral thermally activated delayed fluorescent (TADF) enantiomers, R-TCBN-ImEtPF6 and S-TCBN-ImEtPF6, with intrinsic ionic characteristics were efficiently synthesized by introducing imidazolium hexafluorophosphate to chiral TADF unit. The TADF imidazolium salts exhibited a high photoluminescence quantum yield (PLQY) of up to 92%, a small singlet-triplet energy gap (∆EST) of 0.04 eV, as well as reversible redox properties. Furthermore, the enantiomers showed distinct mirror-image CD and CPL activities with glum values of -3.7×10-3 and +3.4×10-3. Notably, by doping the axial TADF imidazolium salts into achiral TADF sensitizer, sandwich-structured light-emitting electrochemical cells (LECs) without the addition of ionic liquids (ILs) or ionic transition-metal compounds (iTMCs) were fabricated. When driven at 50 A m-2, the LECs displayed an EQE of up to 5.2% and strong circularly polarized electroluminescence (CPEL) with gEL values of +3.3×10-3 and -3.0×10-3. This represents the first CP-LEC based on TADF materials and offers a promising strategy for the development of high-performance CPEL devices.

Neuron-inspired CsPbBr3/PDMS nanospheres for multi-dimensional sensing and interactive displays

Multifunctional materials have attracted tremendous attention in intelligent and interactive devices. However, achieving multi-dimensional sensing capabilities with the same perovskite quantum dot (PQD) material is still in its infancy, with some considering it currently challenging and even unattainable. Drawing inspiration from neurons, a novel multifunctional CsPbBr3/PDMS nanosphere is devised to sense humidity, temperature, and pressure simultaneously with unique interactive responses. The carefully engineered polydimethylsiloxane (PDMS) shell enables the reversible activity of the core CsPbBr3, serving a dual role similar to dendrites in conveying and evaluating external stimuli with high sensitivity. Molecular dynamics analysis reveals that the PDMS shell with proper pore density enhances the conductivity in water and heat, imparting CsPbBr3 with sensitive but reversible properties. By tailoring the crosslinking density of the PDMS shell, nanospheres can surprisingly show customized sensitivity and reversible responses to different level of stimuli, achieving over 95% accuracy in multi-dimensional and wide-range sensing. The regular pressure-sensitive property, discovered for the first time, is attributed to the regular morphology of the nanosphere, the inherent low rigidity of the PDMS shell, and the uniform distribution of the CsPbBr3 core material in combination. This study breaks away from conventional design paradigms of perovskite core-shell materials by customizing the cross-linked density of the shell material. The reversible response mechanism of nanospheres with gradient shell density is deeply explored in response to environmental stimuli, which offers fresh insights into multi-dimensional sensing and interactive display applications.

Facile and Versatile Mechanochemical Synthesis of Indigoid Photoswitches.

We demonstrate the application of mechanochemistry in the synthesis of indolone-based photoswitches (hemiindigos, hemithioindigos, and oxindoles) via Knoevenagel condensation reactions. Utilizing ball-milling and an organic base (piperidine) acting as catalyst and solvent for liquid assisted grinding (LAG) conditions, we achieve rapid, solvent-free transformations, obtaining a set of known and previously unreported photoswitches, including highly functional amino acid-based photoswitches, multichromophoric derivatives and photoswitchable cavitands based on resorcin[4]arenes. The reaction under mechanochemical conditions gives moderate-to-high yields and is highly stereoselective leading to Z-isomers of hemiindigos and hemithioindigos and E-isomers of oxindoles. For selected examples, reversible visible-light photoswiching properties have been demonstrated.

Reversible Thixotropic Rheological Properties of Graphene-Incorporated Epoxy Inks for Self-Standing 3D Printing.

As three-dimensional (3D) printing has emerged as a new manufacturing technology, the demand for high-performance 3D printable materials has increased to ensure broad applicability in various load-bearing structures. In particular, the thixotropic properties of materials, which allow them to flow under applied external forces but resist flowing otherwise, have been reported to enable rapid and high-resolution printing owing to their self-standing and easily processable characteristics. In this context, graphene nanosheets exhibit unique π-π stacking interactions between neighboring sheets, likely imparting self-standing capability to low-viscosity inks. Herein, we develop a thermally curable graphene-incorporated epoxy ink system that exhibits shear-thinning characteristics and upright standing capability owing to its high static yield stress (∼1,680 Pa). The reversible liquid-to-solid phase transition of the composite ink, absent in the pristine epoxy ink, is clearly identified by its viscoelastic properties and dynamic yield stress. This thixotropic composite ink enables the continuous filament printing of 10 stacked layers without the spreading of injected filaments. Significantly, the 3D-printed composite structure, post-thermal curing, exhibits robust structural integrity and is free from weld lines or voids at the stacked interfaces. Combined with the clearly elucidated processing-structure-property relationships of the ink system, our results highlight its potential for a wide spectrum of applications.

Toward the Optimization of a Perovskite-Based Room Temperature Ozone Sensor: A Multifaceted Approach in Pursuit of Sensitivity, Stability, and Understanding of Mechanism.

Metal halide perovskites (MHPs) have attracted significant attention owing to their simple manufacturing process and unique optoelectronic properties. Their reversible electrical or optical property changes in response to oxidizing or reducing environments make them prospective materials for gas detection technologies. Despite advancements in perovskite-based sensor research, the mechanisms behind perovskite-gas interactions, vital for sensor performance, are still inconclusive. This work presents the first evaluation of the sensing performance and long-term stability of MHPs, considering factors such as halide composition variation and Mn doping levels. The research reveals a clear correlation between halide composition and sensing behavior, with Br-rich sensors displaying a p-type response to O3 gas, while Cl-rich counterparts exhibit n-type sensing behavior. Notably, Mn-doping significantly enhances O3 sensing performance by facilitating the gas adsorption process, as supported by both atomistic simulations and experimental evidence. Long-term evaluation of the sensors provides valuable insights into evolving sensing behaviors, highlighting the impact of dynamic instabilities over time. Overall, this research offers insights into optimal halide combination and Mn-doping levels, representing a significant step forward in engineering room temperature perovskite-based gas sensors that are not only low-cost and high-performing but also durable, marking a new era in sensor technology.

PET Imaging of Sphingosine-1-Phosphate Receptor 1 with [18F]TZ4877 in Nonhuman Primates.

The sphingosine-1-phosphate receptor-1 (S1PR1) is involved in regulating responses to neuroimmune stimuli. There is a need for S1PR1-specific radioligands with clinically suitable brain pharmcokinetic properties to complement existing radiotracers. This work evaluated a promising S1PR1 radiotracer, [18F]TZ4877, in nonhuman primates. [18F]TZ4877 was produced via nucleophilic substitution of tosylate precursor with K[18F]/F- followed by deprotection. Brain PET imaging data were acquired with a Focus220 scanner in two Macaca mulatta (6, 13 years old) for 120-180 min following bolus injection of 118-163 MBq [18F]TZ4877, with arterial blood sampling and metabolite analysis to measure the parent input function and plasma free fraction (fP). Each animal was scanned at baseline, 15-18 min after 0.047-0.063 mg/kg of the S1PR1 inhibitor ponesimod, 33 min after 0.4-0.8 mg/kg of the S1PR1-specific compound TZ82112, and 167-195 min after 1 ng/kg of the immune stimulus endotoxin. Kinetic analysis with metabolite-corrected input function was performed to estimate the free fraction corrected total distribution volume (VT/fP). Whole-body dosimetry scans were acquired in 2 animals (1M, 1F) with a Biograph Vision PET/CT System, and absorbed radiation dose estimates were calculated with OLINDA. [18F]TZ4877 exhibited fast kinetics that were described by the reversible 2-tissue compartment model. Baseline [18F]TZ4877 fP was low (<1%), and [18F]TZ4877 VT/fP values were 233-866 mL/cm3. TZ82112 dose-dependently reduced [18F]TZ4877 VT/fP, while ponesimod and endotoxin exhibited negligible effects on VT/fP, possibly due to scan timing relative to dosing. Dosimetry studies identified the critical organs of gallbladder (0.42 (M) and 0.31 (F) mSv/MBq) for anesthetized nonhuman primate. [18F]TZ4877 exhibits reversible kinetic properties, but the low fP value limits reproducible quantification with this radiotracer. S1PR1 is a compelling PET imaging target, and these data support pursuing alternative F-18 labeled radiotracers for potential future human studies.

Research on advanced photoresponsive azobenzene hydrogels with push-pull electronic effects: a breakthrough in photoswitchable adhesive technologies.

Smart materials that adapt to various stimuli, such as light, hold immense potential across many fields. Photoresponsive molecules like azobenzenes, which undergo E-Z photoisomerization when exposed to light, are particularly valuable for applications in smart coatings, light-controlled adhesives, and photoresists in semiconductors and integrated circuits. Despite advances in using azobenzene moieties for stimuli-responsive adhesives, the role of push-pull electronic effects in regulating reversible adhesion remains largely unexplored. In this study, we investigate for the first time photo-controlled hydrogel adhesives of azobenzene with different push-pull electronic groups. We synthesized the monomers 4-methoxyazobenzene acrylate (ABOMe), azobenzene acrylate (ABH), and 4-nitroazobenzene acrylate (ABNO2), and examined their effects on reversible adhesion properties. By incorporating these azobenzene monomers into acrylamide, dialdehyde-functionalized poly(ethylene glycol), and [3-(methacryloylamino)propyl]-trimethylammonium chloride, we prepared ABOMe, ABH, and ABNO2 ionic hydrogels. Our research findings demonstrate that only the ABOMe ionic hydrogel exhibits reversible adhesion. This is due to its distinct transition state mechanism compared to ABH and ABNO2, which enables efficient E-Z photoisomerization and drives its reversible adhesion properties. Notably, the ABOMe ionic hydrogel reveals an outstanding skin adhesion strength of 360.7 ± 10.1 kPa, surpassing values reported in current literature. This exceptional adhesion is attributed to Schiff base reactions, monopole-quadrupole interactions, π-π interactions, and hydrogen bonding with skin amino acids. Additionally, the ABOMe hydrogel exhibits excellent reversible self-healing capabilities, significantly enhancing its potential for injectable medical applications. This research underscores the importance of integrating multifunctional properties into a single system, opening new possibilities for innovative and durable adhesive materials.

Lanthanide-polyoxometalate-based self-erasing luminescent hydrogels with time-dependent and resilient properties for advanced information encryption.

In such an era of information explosion, improving the level of information security is still a challenging task. Self-erasing luminescent hydrogels are becoming ideal candidates for improving the level of information security with simple encryption and decryption methods. Herein, a lanthanide-polyoxometalate-based self-erasing luminescent hydrogel with time-dependent and resilient properties was constructed through a covalent crosslinked network constructed with polyacrylamide and a non-covalent crosslinked network constructed with [2-(methacryloyloxy)ethyl]trimethyl ammonium chloride/Na9DyW10O36, along with doping urease. This acquired hydrogel exhibited reversible luminescence switching properties in the presence of HCl-urea mixed solution. At the same time, obvious changes in the luminescence intensity can be seen on the timescale by modulating the concentrations of HCl and urea/urease. Based on this, the information loaded onto the hydrogel by using a HCl-urea mixed solution self-erased over time, leading to misinformation during this process. The real information can only be recognized at a specific time. Moreover, the information is self-erased permanently, which can avoid secondary leakage of information. In addition, the hydrogel has excellent resilience. The information can be loaded in the stretched state of the hydrogel, resulting in the information only being recognized in the re-stretched state of the hydrogel while the information cannot be recognized in the normal state of the hydrogel. The combination of time-dependent and resilient properties of the hydrogel can further improve the level of information security effectively. This self-erasing luminescent hydrogel with time-dependent and resilient properties has great potential in improving the security of information encryption.

Synthesis and characterization of graphene-based electrocatalysts for the detection of damaged starch ratio

In this study, the detection of damaged starch ratio on synthesized graphene-based electrocatalysts were evaluated based on the iodine oxidation mechanism. Electrocatalysts were synthesized by three different electrochemical exfoliation techniques. Synthesized catalysts were tested in a three-electrode system with electrolytes mixed with flours at different damaged starch ratios (at 16.5, 25 and 30 UCD values). For the electrochemical tests related to iodine absorption, electrolyte solutions were prepared according to Medcalf and Gilles principle; cyclic voltammetry and chronoamperometry were performed at 50 mV/s in the range of 0–1 V vs. SCE; and at a potential of 1 V vs. SCE. The synthesized catalysts showed reversible reaction properties by giving anodic and cathodic peaks of iodine in the three-electrode system. The exfoliated graphene samples were characterized via standard tools such as Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Raman and UV-vis spectroscopy. After all, it was seen that the G2 type electrocatalyst has the highest sensitivity to iodine absorption and potential for use in damaged starch detection.

A Highly Biocompatible Polyoxotungstate with Fenton‐like Reaction Activity for Potent Chemodynamic Therapy of Tumors

Integrating Fenton chemistry and nanomedicine into cancer therapy has significantly promoted the development of chemodynamic therapy (CDT). Nanoscale polyoxometalates (POMs), with their reversible redox properties, exhibit promising potential in developing outstanding CDT drugs by exploring their Fenton‐like catalytic reactivity in tumor environments. However, such research is still in its infancy. In this work, we report the synthesis of a new crystalline antimonotungstate {Dy2Sb2W7O23(OH)(DMF)2(SbW9O33)2} (1, DMF = N, N‐dimethylformamide) with gram‐scale high yield via a facile "one‐pot" solvothermal reaction. 1 exhibits not only a soluble and water‐stable POM nanocluster, but also excellent catalytic activity for hydroxyl radical‐generating Fenton‐like reactions. Further biomedical studies reveal that 1 can trigger cell apoptosis and promote lipid peroxidation, exhibiting high cytotoxicity and selectivity towards B16‐F10 mouse melanoma cancer cells with an IC50 value of 4.75 μM. Especially, 1 can inhibit melanoma growth in vivo with favorable biosafety, achieving a 5.2‐fold reduction in tumor volume and a weight loss of 76.0% at the dose of 70 μg/kg. This research not only demonstrates the immense potential of antimonotungstates in CDT drug development for the first time but also provides new insights and directions for the development of novel anticancer drugs.

A Highly Biocompatible Polyoxotungstate with Fenton-like Reaction Activity for Potent Chemodynamic Therapy of Tumors.

Integrating Fenton chemistry and nanomedicine into cancer therapy has significantly promoted the development of chemodynamic therapy (CDT). Nanoscale polyoxometalates (POMs), with their reversible redox properties, exhibit promising potential in developing outstanding CDT drugs by exploring their Fenton-like catalytic reactivity in tumor environments. However, such research is still in its infancy. In this work, we report the synthesis of a new crystalline antimonotungstate {Dy2Sb2W7O23(OH)(DMF)2(SbW9O33)2} (1, DMF = N, N-dimethylformamide) with gram-scale high yield via a facile "one-pot" solvothermal reaction. 1 exhibits not only a soluble and water-stable POM nanocluster, but also excellent catalytic activity for hydroxyl radical-generating Fenton-like reactions. Further biomedical studies reveal that 1 can trigger cell apoptosis and promote lipid peroxidation, exhibiting high cytotoxicity and selectivity towards B16-F10 mouse melanoma cancer cells with an IC50 value of 4.75 μM. Especially, 1 can inhibit melanoma growth in vivo with favorable biosafety, achieving a 5.2-fold reduction in tumor volume and a weight loss of 76.0% at the dose of 70 μg/kg. This research not only demonstrates the immense potential of antimonotungstates in CDT drug development for the first time but also provides new insights and directions for the development of novel anticancer drugs.

Smart luminescent nanoclusters with dynamic covalent bond for reversible information encryption

AbstractLuminescent nanoclusters (NCs) have attracted much attention because of their superior photophysical properties; however, the design of dynamic NCs with reversible structural change is highly challenging. Herein, we synthesize a kind of dynamic luminescent NCs through Schiff base crosslinking between triethylenetetramine (TETA) and tannic acid at room temperature. The proposed NCs have an excitation‐independent blue emission, and the maximum emission is available at about 458 nm with two excitation centers. Furthermore, the crosslinking degree of the NCs can be effectively adjusted by TETA and their formation is a kinetic‐control process. Most importantly, the proposed NCs show a property of pH‐controlled reversible depolymerization and polymerization, accompanied by a cyclic “on‐off‐on” photoswitching, which is directly attributed to pH‐stimulated reversible C=N bond cleavage and re‐formation. Because of the reversible structure change properties, the dynamic NCs have been well used in reversible information encryption. This new finding provides not only us with a powerful strategy to study the structure–properties relationship of luminescent NCs but also a design idea for constructing smart optical nanomaterials.

Preparation of Electrochromic Hydrogels Based on Anderson-Type POMs-Viologen Compounds with Photochromic and Thermochromic Properties.

Currently, color-changing materials with multiple responses have emerged as a prominent research focus. In this work, two Anderson-type POMs-viologen compounds were successfully synthesized under solvothermal conditions, namely, (1,3-bcby)·[AlMo6(OH)6O18]·(en)0.5·H2O (1) and (1,3-bcby)·[Co2(H2O)6TeMo6O24]·2H2O (2) (1,3-bcby = 1,1'-bis(3-cyanobenzyl)-4,4'-bipyridine dichloride, en = ethylenediamine). Compound 1 shows a two-dimensional supramolecular lattice structure through the hydrogen bonding of dissociated water molecules. In compound 2, by utilizing the connection of Co atoms, a two-dimensional layered framework is constructed. Owing to the widespread application of viologen compounds in color-changing materials, an investigation was performed for exploration of the photochromic and thermochromic responses of 1 and 2 upon exposure to light and heat. The study reveals that compound 1 exhibits an exceptional reversible photochromic property, while compound 2 demonstrated equally impressive thermochromic behavior. The filter paper prepared by compound 1 was successfully applied to ink-free, erasable printing. Furthermore, ECH-1 and ECH-2 (two types of electrochromic hydrogels), were prepared through varying concentrations of compounds 1 and 2. Notably, ECH-1 exhibits a rapid response time and substantial coloring efficiency. ECH-1 and ECH-2 have been integrated into smart windows, demonstrating their capacity to modulate indoor temperatures effectively.

Wood-based phase change energy storage composite material with reversible thermochromic properties

With the continuous increase in global energy demand and environmental challenges, the efficient utilization and storage of energy have become critical areas of scientific research. This study presents the preparation and performance assessment of a wood-based phase change composite (TPW) with reversible thermochromic properties. Thermogravimetric analysis (TG) confirmed thermal stability across 26 °C to 270 °C, while differential scanning calorimetry (DSC) demonstrated that TPW has good thermal cycling performance, and suitable phase change temperature at about 34 °C. Thermal insulation tests showed that TPW can reduce heat exchange between inside and outside environment, maintaining the internal temperature for longer time. Below the transition temperature, the material displays a bluish-purple color, transitioning to light yellow upon heating, with a notable color difference (ΔE⁎) increase from 3.46 to 67.89. Since the phase transition temperature close to human body temperature, enhances TPW’s compatibility for applications in home decor, temperature indicators, and anti-counterfeit labeling. This research contributes novel insights and foundational data for advancing wood-based functional materials in sustainable applications.

PP3.6 – 00064 Novel synthesized protein kinase C modulators show enhanced HIV latency reversal properties and synergize with a BET bromodomain inhibitor

PP3.6 – 00064 Novel synthesized protein kinase C modulators show enhanced HIV latency reversal properties and synergize with a BET bromodomain inhibitor

Enzymatically deamidated pork myofibrillar protein can form thermo-reversible gels.

By using protein-glutaminase (PG) deamidation, thermo-reversible gel of pork myofibrillar protein (PMP) can be prepared. This study aims to reveal the connection between PMP thermo-reversible gel and the coiled-coil. The research explores how the water-holding capacity and reversibility of these gels improve with increased deamidation time. At a deamidation duration of 4 h, the gel strength reaches its peak. Moreover, PG deamidation significantly enhances the α-helix content to over 60 %. This change boosts the likelihood of α-helix interactions while increasing coiled helix content. Notably, the interference experiment of trifluoroethanol suggests that a high concentration of coiled coils is essential for imparting reversible properties to the deamidated myofibrillar protein gel. This research contributes to the advancement of protein micro-modification in the custom manufacturing of muscle protein-based soft meal products.