We propose to develop a reusable electro-chemical sensor for selective detection of 4-nitrophenol (4-NP), based on molecularly imprinted polymer (MIP) technology. MIP technology is the design of an artificial receptor with high selectivity for a specific analyte. Our approach combines N-isopropylacrylamide (NIPAM) backbone polymer with different functional monomers to improve the selectivity and sensitivity of 4-nitrophenol (4-NP) detection.The key points of our sensor polymer are:1) reduced amount of covalent crosslinker methylene bis-acrylamide (MBA) to increase rebinding kinetics and affinity and 2) modified polymer backbone with an electroactive block deposited on a gold surface. To get rapid high affinity template rebinding, we used a reduced amount of covalent crosslinker and used noncovalent crosslinking based on acid-base and hydrophobic interactions. To form acid-base crosslinking, we used 4-vinylpyridine (4-VP) as the basic monomer and methacrylic acid (MAA) as acidic monomer.In MIPs synthesis part, our strategy takes the advantage of reversible addition fragmentation-chain transfer (RAFT) polymerization to control the polymer chain length and modify the terminus as poly-ferrocene methyl methacrylate (FMMA), an electroactive block. The RAFT agent contains R group and Z group which are at different ends of polymer. The Z side is reduced to a thiol and deposited on gold. The PolyFMMA block is on the R side. RAFT agent, FMMA monomers and initiator were added to a batch system to synthesis the first electroactive block. Then, all chemicals including backbone monomers, functional monomers, crosslinkers and templates (4-NP) were added to form the major polymer block. After synthesis, templated-MIPs were dialyzed to remove the template for further target selective detection.One of the major challenges of our sensor is polymer tangling which affect measurement stability. To reduce polymer tangling effects, we dissolved the template-removed MIPs into tetrahydrofuran (THF) and controlled the gold electrode surface coverage. We deposited MIPs on an additional flat gold chip and used atomic force microscope (AFM) to characterize the surface coverage distribution.The modified gold electrode can be used in electrical impedance spectroscopy (EIS) or cyclic voltammetry (CV) to measure charge transfer distance before and after template rebinding to detect unknown concentration target analytes.