Cycles Of Retraction Research Articles

Experimental and Model Studies of Various Size Water Droplet Impacting on a Hydrophobic Surface

Abstract Impacting droplet on a hydrophobic surface is investigated and droplet size effect on impacting properties is examined. Liquid pressure variation inside droplet is numerically simulated in the impacting and rebounding periods. Droplet motion on impacted hydrophobic surface is monitored using a high-speed recording system. We showed that predictions and high-speed data for droplet shape and geometric features appear to be almost identical in the spreading and retraction of the droplet on sample surface. Increased volume of droplet gives rise to the peak pressure enhancement in droplet liquid during impact. The maximum droplet height remains larger for large volume droplets in both spreading and retraction cycles. Increasing size of droplet enlarges the wetting diameter on the impacted surface during droplet deformation on sample surfaces. The rate of peak velocity of the spreading surface of the droplet is faster for larger droplets as compared to that corresponding to small droplets. The ratio of spreading period over the retraction period of the droplet becomes small for droplets with small size.

Competitive binding of independent extension and retraction motors explains the quantitative dynamics of type IV pili

Type IV pili (TFP) function through cycles of extension and retraction. The coordination of these cycles remains mysterious due to a lack of quantitative measurements of multiple features of TFP dynamics. Here, we fluorescently label TFP in the pathogen Pseudomonas aeruginosa and track full extension and retraction cycles of individual filaments. Polymerization and depolymerization dynamics are stochastic; TFP are made at random times and extend, pause, and retract for random lengths of time. TFP can also pause for extended periods between two extension or two retraction events in both wild-type cells and a slowly retracting PilT mutant. We developed a biophysical model based on the stochastic binding of two dedicated extension and retraction motors to the same pilus machine that predicts the observed features of the data with no free parameters. We show that only a model in which both motors stochastically bind and unbind to the pilus machine independent of the piliation state of the machine quantitatively explains the experimentally observed pilus production rate. In experimental support of this model, we show that the abundance of the retraction motor dictates the pilus production rate and that PilT is bound to pilus machines even in their unpiliated state. Together, the strong quantitative agreement of our model with a variety of experiments suggests that the entire repetitive cycle of pilus extension and retraction is coordinated by the competition of stochastic motor binding to the pilus machine, and that the retraction motor is the major throttle for pilus production.

Selection Methodology of an Electric Actuator for Nose Landing Gear of a Light Weight Aircraft

Landing gear system of an aircraft enables it to take off and land with safety and comfort. Because of the horizontal and vertical velocity of aircraft, upon landing, the complete aircraft undergoes different forcing functions in the form of the impact force that is absorbed by landing gears, shock absorbers, and actuators. In this research, a selection methodology has been proposed for an electrical actuator to be installed in the retraction mechanism of nose landing gear of an aircraft having 1600 kg gross takeoff weight. Nose landing gear and its associated components, like strut and shock absorbers, were modeled in CAD software. Analytical expressions were then developed in order to calculate the actuator stroke, translational velocity, force, and power for complete cycle of retraction, and some were subsequently compared with the computational results that were obtained using MSC ADAMS®. Air in the oleo-pneumatic shock absorber of nose landing gear was modeled as a nonlinear spring with equivalent spring constant, whereas hydraulic oil was modeled as a nonlinear damper with equivalent damping constant. The nose landing gear system was modeled as a mass-spring-damper system for which a solution for sinusoidal forcing functions is proposed. Finally, an electrical actuator has been selected, which can retract and extend nose landing gear, meeting all of the constraints of aircraft, like fuselage space, aircraft ground clearance, locking loads, power consumption, retraction and extension time, and dynamic response of aircraft. It was found that the selection of an electrical actuator is based upon the quantification of forces transmitted to electrical actuator during one point load at gross takeoff weight. The ability of retraction and extension time, as dictated by Federal Aviation Regulation, has also been given due consideration in the proposed methodology as significant criteria. The proposed system is now in the process of ground testing, followed by flight testing in the near future.

Actin-Membrane Release Initiates Cell Protrusions.

Despite the well-established role of actin polymerization as a driving mechanism for cell protrusion, upregulated actin polymerization alone does not initiate protrusions. Using a combination of theoretical modeling and quantitative live-cell imaging experiments, we show that local depletion of actin-membrane links is needed for protrusion initiation. Specifically, we show that the actin-membrane linker ezrin is depleted prior to protrusion onset and that perturbation of ezrin's affinity for actin modulates protrusion frequency and efficiency. We also show how actin-membrane release works in concert with actin polymerization, leading to a comprehensive model for actin-driven shape changes. Actin-membrane release plays a similar role in protrusions driven by intracellular pressure. Thus, our findings suggest that protrusion initiation might be governed by a universal regulatory mechanism, whereas the mechanism of force generation determines the shape and expansion properties of the protrusion.

Syngeneic Transplantation of Rat Olfactory Stem Cells in a Vein Conduit Improves Facial Movements and Reduces Synkinesis after Facial Nerve Injury.

Posttraumatic facial paralysis is a disabling condition. Current surgical management by faciofacial nerve suture provides limited recovery. To improve the outcome, the authors evaluated an add-on strategy based on a syngeneic transplantation of nasal olfactory stem cells in a rat model of facial nerve injury. The main readouts of the study were the recording of whisking function and buccal synkinesis. Sixty rats were allocated to three groups. Animals with a 2-mm facial nerve loss were repaired with a femoral vein, filled or not with olfactory stem cells. These two groups were compared to similarly injured rats but with a faciofacial nerve suture. Olfactory stem cells were purified from rat olfactory mucosa. Three months after surgery, facial motor performance was evaluated using video-based motion analysis and electromyography. Synkinesis was assessed by electromyography, using measure of buccal involuntary movements during blink reflex, and double retrograde labeling of regenerating motoneurons. The authors' study reveals that olfactory stem cell transplantation induces functional recovery in comparison to nontransplanted and faciofacial nerve suture groups. They significantly increase (1) maximal amplitude of vibrissae protraction and retraction cycles and (2) angular velocity during protraction of vibrissae. They also reduce buccal synkinesis, according to the two techniques used. However, olfactory stem cell transplantation did not improve axonal regrowth of the facial nerve, 3 months after surgery. The authors show here that the adjuvant strategy of syngeneic transplantation of olfactory stem cells improves functional recovery. These promising results open the way for a phase I clinical trial based on the autologous engraftment of olfactory stem cells in patients with a facial nerve paralysis.

La era del vinilo en Argentina: Expansión y retracción de la industria discográfica nacional, 1964–1984

This article systematizes and analyzes statistical reports from the Instituto Nacional de Estadísticas y Censos (INDEC) on music records production in Argentina between 1964 and 1984. The first section presents the evidence of the economic cycle of expansion and retraction that the Argentine recording industry experienced throughout the period, in full accordance with Latin American and global dynamics. The second section looks to understand this economic process not as a mere “cold fact” but also as both a factor and a sign of profound sociocultural transformations. The coincidence between the period considered by INDEC and the emergent phenomenon of youth music can be seen, in this regard, as completely symptomatic. Similar considerations should be made regarding the increasing importance of the recording format that the new rock culture chose as its own: the LP. As in many other places, Argentine youth music and culture represented one of the most distinctive expressions of the new economic and sociocultural forms of global capitalism in the second half of the twentieth century.

PilY1 and minor pilins form a complex priming the type IVa pilus in Myxococcus xanthus

Type IVa pili are ubiquitous and versatile bacterial cell surface filaments that undergo cycles of extension, adhesion and retraction powered by the cell-envelope spanning type IVa pilus machine (T4aPM). The overall architecture of the T4aPM and the location of 10 conserved core proteins within this architecture have been elucidated. Here, using genetics, cell biology, proteomics and cryo-electron tomography, we demonstrate that the PilY1 protein and four minor pilins, which are widely conserved in T4aP systems, are essential for pilus extension in Myxococcus xanthus and form a complex that is an integral part of the T4aPM. Moreover, these proteins are part of the extended pilus. Our data support a model whereby the PilY1/minor pilin complex functions as a priming complex in T4aPM for pilus extension, a tip complex in the extended pilus for adhesion, and a cork for terminating retraction to maintain a priming complex for the next round of extension.

Viscoelasticity in simple indentation-cycle experiments: a computational study

Instrumented indentation has become an indispensable tool for quantitative analysis of the mechanical properties of soft polymers and biological samples at different length scales. These types of samples are known for their prominent viscoelastic behavior, and attempts to calculate such properties from the indentation data are constantly made. The simplest indentation experiment presents a cycle of approach (deepening into the sample) and retraction of the indenter, with the output of the force and indentation depth as functions of time and a force versus indentation dependency (force curve). The linear viscoelastic theory based on the elastic–viscoelastic correspondence principle might predict the shape of force curves based on the experimental conditions and underlying relaxation function of the sample. Here, we conducted a computational analysis based on this theory and studied how the force curves were affected by the indenter geometry, type of indentation (triangular or sinusoidal ramp), and the relaxation functions. The relaxation functions of both traditional and fractional viscoelastic models were considered. The curves obtained from the analytical solutions, numerical algorithm and finite element simulations matched each other well. Common trends for the curve-related parameters (apparent Young’s modulus, normalized hysteresis area, and curve exponent) were revealed. Importantly, the apparent Young’s modulus, obtained by fitting the approach curve to the elastic model, demonstrated a direct relation to the relaxation function for all the tested cases. The study will help researchers to verify which model is more appropriate for the sample description without extensive calculations from the basic curve parameters and their dependency on the indentation rate.

Intraseasonal Drainage Network Dynamics in a Headwater Catchment of the Italian Alps

AbstractIn the majority of existing studies, streams are conceived as static objects that occupy predefined regions of the landscape. However, empirical observations suggest that stream networks are systematically and ubiquitously featured by significant expansion/retraction dynamics produced by hydrologic and climatic variability. This contribution presents novel empirical data about the active drainage network dynamics of a 5 km2 headwater catchment in the Italian Alps. The stream network has been extensively monitored with a biweekly temporal resolution during a field campaign conducted from July to November 2018. Our results reveal that, in spite of the wet climate typical of the study area, more than 70% of the observed river network is temporary, with a significant presence of disconnected reaches during wet periods. Available observations have been used to develop a set of simple statistical models that were able to properly reconstruct the dynamics of the active stream length as a function of antecedent precipitation. The models suggest that rainfall timing and intensity represent major controls on the stream network length, while evapotranspiration has a minor effect on the observed intraseasonal changes of drainage density. Our results also indicate the presence of multiple network expansion and retraction cycles that simultaneously operate at different time scales, in response to distinct hydrological processes. Furthermore, we found that observed spatial patterns of network dynamics and unchanneled lengths are related to the underlying heterogeneity of geological attributes. The study offers novel insights on the physical mechanisms driving stream network dynamics in low‐order alpine catchments.

Traction Forces Control Cell-Edge Dynamics and Mediate Distance Sensitivity during Cell Polarization.

Traction forces are generated by cellular actin-myosin system and transmitted to the environment through adhesions. They are believed to drive cell motion, shape changes, and extracellular matrix remodeling [1-3]. However, most of the traction force analysis has been performed on stationary cells, investigating forces at the level of individual focal adhesions or linking them to static cell parameters, such as area and edge curvature [4-10]. It is not well understood how traction forces are related to shape changes and motion, e.g., forces were reported to either increase or drop prior to cell retraction [11-15]. Here, we analyze the dynamics of traction forces during the protrusion-retraction cycle of polarizing fish epidermal keratocytes and find that forces fluctuate together with the cycle, increasing during protrusion and reaching maximum at the beginning of retraction. We relate force dynamics to the recently discovered phenomenological rule [16] that governs cell-edge behavior during keratocyte polarization: both traction forces and probability of switch from protrusion to retraction increase with the distance from the cell center. Diminishing forces with cell contractility inhibitor leads to decreased edge fluctuations and abnormal polarization, although externally applied force can induce protrusion-retraction switch. These results suggest that forces mediate distance sensitivity of the edge dynamics and organize cell-edge behavior, leading to spontaneous polarization. Actin flow rate did not exhibit the same distance dependence as traction stress, arguing against its role in organizing edge dynamics. Finally, using a simple model of actin-myosin network, we show that force-distance relationship might be an emergent feature of such networks.

Biointerface anisotropy modulates migration of breast cancer cell

Migration of cancer cell is a cyclic process, which involves dynamic interaction between extracellular biointerface and cellular responds. In tumors, collagen as extracellular matrix reorganizes biointerface from curl and isotropic fibers to straightened and anisotropic fibers during tumorigenesis, yet how cell migration respond to topography of biointerface is unknown. In this research, we introduced a facile fabrication method on nanofibers of varying topography, which was mimicking the alignment of extracellular nanofibers, to examine the change of cytoskeleton during cell migration. We took advantage of breast carcinoma cell line (MDA-MB-231) for time-lapse imaging analysis. We found that biointerface anisotropy modulated morphology of cell and mediated the pattern of migration. Morphologically, cells on anisotropic nanofiber showed extending spindle shape. The trajectories of migration templated the topographic pattern on biointerface. Besides, aligned nanofiber induced caterpillar-like model of migration through protrusion - retraction cycle, which was indicated by periodical variation of aspect ratio and velocity of cells. The biointerface anisotropy triggered vimentin filaments and microtubule networks preferentially oriented along the alignment of nanofibers. And the velocity of cell mobility by vimentin, β-catenin or CDC42 knockdown was significantly enhanced on aligned nanofibers. Thus, we implied that biointerface anisotropy modulated migration of breast cancer cell and it associated with reorganization of cytoskeleton filaments.

Competitive Substrate Binding Coordinates the Two Antagonistic Motors of the Bacterial Type IV Pilus

The bacterial type IV pilus is an intricate nano-machine that coordinates two antagonistic molecular motors to extend and retract a semi-flexible polymer to the environment. Filament retraction is driven by the strongest molecular motor known and used for a variety of tasks such as uptake of DNA, surface sensing, and motility. However, how both motors are coordinated to dynamically extend and retract pili is unknown. To fill this void, we genetically engineered a fluorescent label for the pilus fiber. This enables us to characterize the dynamic cycles of extension and retraction of the pilus in an unprecedented way. Careful characterization of pilus dynamics reveals that pili are short and individual cells make a new pilus every 10 seconds. This is in stark contrast to the current dogma of the field that was shaped by static cyro electron images. To explain this discrepancy, we introduce a simplistic three-state model that describes the mutually exclusive binding/unbinding of the motor proteins to the pilus machine by a Poisson process. We combined live cell super-resolution microscopy and Monte-Carlo simulations to determine the rate constants of the model. We explain how these rates determine biological function such as pilus length and rate of pilus assembly. Intriguingly, we find that the major throttle of pilus production is the unbinding step of the retraction motor. Furthermore, we measured pilus activity with and without surface contact by combining fluorescence microscopy and line-scanning optical tweezers. Cells show similar levels of pilus activity, overturning the current model that surface contact drives pilus retraction. Together, our results shape a comprehensive picture of the physical processes that determine pilus activity and suggest that the pilus was optimized for multitasking of different tasks such as DNA uptake and motility.

The type IV pilus protein PilU functions as a PilT-dependent retraction ATPase.

Type IV pili are dynamic cell surface appendages found throughout the bacteria. The ability of these structures to undergo repetitive cycles of extension and retraction underpins their crucial roles in adhesion, motility and natural competence for transformation. In the best-studied systems a dedicated retraction ATPase PilT powers pilus retraction. Curiously, a second presumed retraction ATPase PilU is often encoded immediately downstream of pilT. However, despite the presence of two potential retraction ATPases, pilT deletions lead to a total loss of pilus function, raising the question of why PilU fails to take over. Here, using the DNA-uptake pilus and mannose-sensitive haemagglutinin (MSHA) pilus of Vibrio cholerae as model systems, we show that inactivated PilT variants, defective for either ATP-binding or hydrolysis, have unexpected intermediate phenotypes that are PilU-dependent. In addition to demonstrating that PilU can function as a bona fide retraction ATPase, we go on to make the surprising discovery that PilU functions exclusively in a PilT-dependent manner and identify a naturally occurring pandemic V. cholerae PilT variant that renders PilU essential for pilus function. Finally, we show that Pseudomonas aeruginosa PilU also functions as a PilT-dependent retraction ATPase, providing evidence that the functional coupling between PilT and PilU could be a widespread mechanism for optimal pilus retraction.

Type IV pili: dynamics, biophysics and functional consequences.

The surfaces of many bacteria are decorated with long, exquisitely thin appendages called type IV pili (T4P), dynamic filaments that are rapidly polymerized and depolymerized from a pool of pilin subunits. Cycles of pilus extension, binding and retraction enable T4P to perform a phenomenally diverse array of functions, including twitching motility, DNA uptake and microcolony formation. On the basis of recent developments, a comprehensive understanding is emerging of the molecular architecture of the T4P machinery and the filament it builds, providing mechanistic insights into the assembly and retraction processes. Combined microbiological and biophysical approaches have revealed how T4P dynamics influence self-organization of bacteria, how bacteria respond to external stimuli to regulate T4P activity for directed movement, and the role of T4P retraction in surface sensing. In this Review, we discuss the T4P machine architecture and filament structure and present current molecular models for T4P dynamics, with a particular focus on recent insights into T4P retraction. We also discuss the functional consequences of T4P dynamics, which have important implications for bacterial lifestyle and pathogenesis.

A role for actomyosin contractility in Notch signaling

BackgroundNotch-Delta signaling functions across a wide array of animal systems to break symmetry in a sheet of undifferentiated cells and generate cells with different fates, a process known as lateral inhibition. Unlike many other signaling systems, however, since both the ligand and receptor are transmembrane proteins, the activation of Notch by Delta depends strictly on cell-cell contact. Furthermore, the binding of the ligand to the receptor may not be sufficient to induce signaling, since recent work in cell culture suggests that ligand-induced Notch signaling also requires a mechanical pulling force. This tension exposes a cleavage site in Notch that, when cut, activates signaling. Although it is not known if mechanical tension contributes to signaling in vivo, others have suggested that this is how endocytosis of the receptor-ligand complex contributes to the cleavage and activation of Notch. In a similar way, since Notch-mediated lateral inhibition at a distance in the dorsal thorax of the pupal fly is mediated via actin-rich protrusions, it is possible that cytoskeletal forces generated by networks of filamentous actin and non-muscle myosin during cycles of protrusion extension and retraction also contribute to Notch signaling.ResultsTo test this hypothesis, we carried out a detailed analysis of the role of myosin II-dependent tension in Notch signaling in the developing fly and in cell culture. Using dynamic fluorescence-based reporters of Notch, we found that myosin II is important for signaling in signal sending and receiving cells in both systems—as expected if myosin II-dependent tension across the Notch-Delta complex contributes to Notch activation. While myosin II was found to contribute most to signaling at a distance, it was also required for maximal signaling between adjacent cells that share lateral contacts and for signaling between cells in culture.ConclusionsTogether these results reveal a previously unappreciated role for non-muscle myosin II contractility in Notch signaling, providing further support for the idea that force contributes to the cleavage and activation of Notch in the context of ligand-dependent signaling, and a new paradigm for actomyosin-based mechanosensation.

A Comprehensive View of Type IV Pilus Retraction Forces across the Bacterial Domain

Type IV pili are long flexible polymeric appendages emanating from many bacteria. They are playing key roles in many bacterial functions: DNA uptake, motility, colonization, infection, biofilm formation, aggregation. A central feature of these appendages is their ability to undergo cycles of elongation and retraction enabling them to exert physical forces on their surroundings. In order to gain insight in the differences and commonalities of the biophysical characteristics of those retraction forces across evolution, we have measured, combining different techniques, the retraction forces of type IV pili in a wide range of bacterial genus (Neisseria, Escherichia, Vibrio, Synechocystis, Streptococcus). Of particular interest is the role that the so-called retraction ATPase (pilT) plays in the biophysical characteristics of the retraction forces. The direct comparison of the retraction forces in different mutants of those very different genus will help ascertain the common biophysical features of the Type IV pilus machinery across bacteria.

Time-resolved ultrastructure of the cortical actin cytoskeleton in dynamic membrane blebs

Membrane blebbing accompanies various cellular processes, including cytokinesis, apoptosis, and cell migration, especially invasive migration of cancer cells. Blebs are extruded by intracellular pressure and are initially cytoskeleton-free, but they subsequently assemble the cytoskeleton, which can drive bleb retraction. Despite increasing appreciation of physiological significance of blebbing, the molecular and, especially, structural mechanisms controlling bleb dynamics are incompletely understood. We induced membrane blebbing in human HT1080 fibrosarcoma cells by inhibiting the Arp2/3 complex. Using correlative platinum replica electron microscopy, we characterize cytoskeletal architecture of the actin cortex in cells during initiation of blebbing and in blebs at different stages of their expansion-retraction cycle. The transition to blebbing in these conditions occurred through an intermediate filopodial stage, whereas bleb initiation was biased toward filopodial bases, where the cytoskeleton exhibited local weaknesses. Different stages of the bleb life cycle (expansion, pausing, and retraction) are characterized by specific features of cytoskeleton organization that provide implications about mechanisms of cytoskeleton assembly and bleb retraction.

A Biologist-Friendly Method to Analyze Cross-Correlation Between Protrusion Dynamics and Membrane Recruitment of Actin Regulators.

During mesenchymal cell motility, various actin regulators are recruited to the leading edge with exquisite precision in time and space to generate protrusion and retraction cycles. We present here an automated method, named CorRecD (from Correlation Recruitment Dynamics), which quantifies cell edge dynamics, protein recruitment and analyze their cross-correlation. The Wave Regulatory Complex (WRC), a master driver of protrusions, is used as a case-of-study. This biologist-friendly method relies on free software tools and can be applied to any fluorescently tagged protein of interest.

Numerical simulation of the hair formation –modeling of hair cycle

In the recent years, the fields of study of anti-aging, health and beauty, cosmetics, and hair diseases have attracted significant attention. In particular, human hair is considered to be an important aspect with regard to an attractive appearance. To this end, many workers have sought to understand the formation mechanism of the hair root. However, observing growth in the hair root is difficult, and a detailed mechanism of the process has not yet been elucidated. Hair repeats growth, retraction, and pause cycles (hair cycle) in a repetitive process. In the growth phase, hair is formed through processes of cell proliferation and differentiation (keratinization). During the retraction phase, hair growth stops, and during the resting period, hair fall occurs and new hair grows. This hair cycle is believed to affect the elongation rate, thickness, strength, and shape of hair. Therefore, in this study, we introduce a particle model as a new method to elucidate the unknown process of hair formation, and to model the hair formation process accompanying the proliferation and differentiation of the hair root cells in all three dimensions. In addition, to the growth period, the retraction and the resting periods are introduced to realize the hair cycle using this model.

Optimization of a Main Landing Gear Locking Mechanism Using Bifurcation Analysis

A key part of the main landing gear of a civil aircraft is its locking mechanism that holds the gear in the deployed or downlocked state. The locking is driven by a spring mechanism and its release by the unlock actuator. This paper considers this mechanism in terms of its stability and the locking and unlocking forces required for downlocking. Using numerical continuation to solve these equations, the effect of the unlock force on the main landing gear retraction cycle was analyzed. The variation of a fold bifurcation point, which indicates the transition between the locked state and the unlocked state, gives further insight into the required unlock force that governs the sizing of the unlock actuator. Moreover, some important information, such as the critical position for the lock links’ stops, the unlock position, and the unlock force, is discussed using the bifurcation diagrams for the main landing gear retraction/extension cycle. Then, the effect of three key geometry parameters of the locking spring (the spring stiffness, unstrained spring length, and spring attachment point) on the critical over-center angle and the unlock force are investigated. Finally, an optimization of the critical unlock force is carried out with a constraint on the initial over-center angle.