Results Of Routine Examination Research Articles

Evaluation of vascular invasion in pancreatic head tumors and its impact on radical surgical treatment

Objective. To evaluate the informativeness and sensitivity of radiological criteria for vascular invasion in pancreatic head tumors in comparison with intraoperative data and results of routine pathological examination. Materials and methods. The study included 98 patients with ductal adenocarcinoma of the pancreatic head with suspected venous invasion and spread of tumor growth along the vessels of the portomesenteric axis, who were operated on at the Department of Pancreatic and Bile Duct Surgery of the National Research Center of Surgery and Transplantation named after O. A. Shalimov in the period from 2019 to 2023. Group 1 included 51 (52.0%) patients who underwent radical surgery with resection of the superior mesenteric/hepatic portal vein and extended lymph and neurodissection, and group 2 included 47 (48.0%) patients who had positive vascular margins of pancreatic resection according to routine pathological examination. Diagnostic criteria for vascular alterations were developed. A comparative analysis of the informativeness of the developed criteria and the results of a routine pathological examination was performed. The edges of the pancreatic resection were evaluated, including the neck of the gland, common bile duct, duodenum/gastric duct, and the bed of the superior mesenteric/hepatic portal vein and superior mesenteric artery separately. R1 status was defined as the presence of tumor cells at a distance of less than 1 mm to the resection margin. Results. According to the criteria of the National Comprehensive Cancer Network (NCCN) 2023.2, 61 (62.2%) patients had a marginally resectable stage of ductal adenocarcinoma of the pancreatic head and tumor contact with the superior mesenteric/hepatic veins according to multislice computed tomography of the abdominal cavity. In group 1, there were 36 (70.6%) such patients, in group 2 – 25 (53.2%). According to the proposed radiologic criteria for vascular invasion, tumor contact with the superior mesenteric/hepatic portal veins was suspected in 76 (77.6%) patients, which is higher than in the standard approach. Determination of vascular alterations according to the results of our study is a more reliable method for diagnosing invasion of the superior mesenteric/hepatic portal vein (χ2 = 5.46, p = 0.019) and predicting the performance of radical surgery, including vascular resection. Conclusions. The proposed criteria for the radiological diagnosis of vascular invasion at the preoperative stage increased the sensitivity of this method from 62.2 to 77.6%. The most frequent radiological signs of vascular alteration are vascular wall irregularity (47.7%) and parapancreatic tissue density (62.5%). In the presence of any of the radiological signs of vascular alteration, resection of the affected area of the superior mesenteric/hepatic portal vein with extended lymph and neurodissection is indicated, which allows a statistically significant increase in the number of R0 resections.

Clinical significance of PLT for diagnosis and treatment monitoring in imported malaria

To evaluate the clinical significance of PLT, MPV, and PDW in monitoring malaria treatment efficacy and predicting disease progression. A total of 31 patients with imported malaria were selected as the observation group, while 31 non-malaria patients with fever were selected as controls. The observation group was subdivided into a complication group and a non-complication group according to the occurrence of complications during treatment. Additionally, on the 1st day (within 24 h), the 3rd day, and the 5th day following admission, a comprehensive blood routine examination, Plasmodium microscopic examination, and colloidal gold assay were conducted. The blood routine examination results were compared before and after treatment among patients in the observation group and the control group. Moreover, the study involved dynamic monitoring and analysis of the levels and variations in PLT, MPV, and PDW within both the complication group and the non-complication group. The Plasmodium density was negatively correlated with PLT before treatment. There were significant differences were observed in PLT, MPV, and PDW (P < 0.05) within the observation group before and after treatment. Notably, there were no significant alterations in red blood cell (RBC), hemoglobin (Hb), and white blood cell (WBC) counts (P > 0.05) within the observation group before and after treatment. The PLT, MPV, and PDW levels in the complication group and the non-complication group exhibited an upward trend after treatment. Further, the PLT of patients in the complication group was significantly lower than that in the non-complication group. Additionally, the PLT, MPV, and PDW levels in the complication group and the non-complication group increased gradually from the time of admission to the 3rd and 5th day of treatment. Notably, the PLT in the complication group was consistently lower than that in the non-complication group. The continuous monitoring of PLT, MPV, and PDW changes plays a crucial role in assessing malaria treatment efficacy and prognosis in these individuals.

A Rapid In Vivo Toxicity Assessment Method for Antimicrobial Peptides.

Antimicrobial peptides (AMPs) represent a promising antibiotic alternative to overcome drug-resistant bacteria by inserting into the membrane of bacteria, resulting in cell lysis. However, therapeutic applications of AMPs have been hindered by their ability to lyse eukaryotic cells. GF-17 is a truncated peptide of LL-37, which has perfect amphipathicity and a higher hydrophobicity, resulting in higher haemolytic activity. However, there is no significant difference in the cytotoxicity against human lung epithelial cells between the GF-17 and LL-37 groups, indicating that there are significant differences in the sensitivity of different human cells to GF-17. In this study, LL-37 and GF-17 were administered to mouse lungs via intranasal inoculation. Blood routine examination results showed that LL-37 did not affect the red blood cells, platelet, white blood cells and neutrophil counts, but GF-17 decreased the white blood cells and neutrophil counts with the increasing concentration of peptides. GF-17-treated mice suffer a body weight loss of about 2.3 g on average in 24 h, indicating that GF-17 is highly toxic to mice. The total cell counts in the bronchoalveolar lavage fluid from GF-17-treated mice were 4.66-fold that in the untreated group, suggesting that GF-17 treatment leads to inflammation in the lungs of mice. Similarly, the histological results showed the infiltration of neutrophils in the lungs of GF-17-treated mice. The results suggest that the administration of GF-17 in the lungs of mice does not affect the red blood cells and platelet counts in the blood but promotes neutrophil infiltration in the lungs, leading to an inflammatory response. Therefore, we established a mouse acute lung injury model to preliminarily evaluate the in vivo toxicity of AMPs. For AMPs with a clinical application value, systematic research is still needed to evaluate their acute and long-term toxicity.

Application value of 18F-FDG PET/CT in primary spleen angiosarcoma with liver metastasis: a case report and literature review

BackgroundPrimary splenic angiosarcoma (PSA) is a rare neoplasm. It is a malignant tumor derived from endothelial cells of the splenic sinuses. PSA has an unknown etiology, a high degree of malignancy, easy early metastasis, atypical clinical symptoms and imaging findings, and difficult early diagnosis. This paper reports the 18F-FDG PET/CT findings of a case of PSA with intrahepatic metastasis; summarizes its clinical, imaging, and pathological data; and reviews the literature.Case descriptionA 64-year-old male patient presented with left lower abdominal distending pain without obvious causes on 13 March 2022. The pain was persistent and dull and worsened after sitting and eating. Blood routine examination results were RBC ↓ 3.33 × 1012/L, WBC ↑ 12.32 × 109/L, and PLT ↓ 40 × 109/L. The tumor markers indicated CA125 ↑ 47.0 U/ml, AFP (−), CEA (−), CA199 (−), and CA724 (−). Non-contrast-enhanced CT scan of the abdomen showed that the spleen was significantly enlarged in volume and irregular in shape and had multiple nodules and clumpy low-density shadows, unclear boundaries, uneven density, and multiple necrotic areas. Enhanced CT showed diffuse uneven mild enhancement of the spleen, and the degree of enhancement increased with time. Multiple nodular low-density shadows were seen in the liver, which were slightly enhanced by the enhanced scan.18F-FDG PET/CT showed multiple nodular and massive lesions in the spleen with multiple necrotic areas. There were multiple nodular lesions in the liver, the level of FDG metabolism increased, the SUVmax of the spleen lesions was 9.0, and the SUVmax of the liver lesions was 5.6. The 18F-FDG PET/CT diagnosis was splenic malignancy with liver metastasis. Finally, after a multidisciplinary discussion, it was decided to perform laparoscopic total splenectomy and portal vein infusion chemotherapy. Pathological examination showed that the tumor cells were round, oval, or fusiform, with obvious atypia, arranged into a cable or anastomosed vascular lumen. The final diagnosis was primary splenic angiosarcoma with massive necrosis. After surgery, the patient received antitumor combined therapy and died 5 months later.ConclusionThe incidence of PSA is very low, and its clinical and radiological manifestations lack specificity. 18F-FDG PET/CT imaging has a certain diagnostic value for PSA and significant utility in preoperative staging, guiding biopsy procedures, evaluating postoperative treatment response, and monitoring disease recurrence. PSA should be considered in the presence of a space-occupying lesion within the spleen that exhibits necrotic areas, shows progressive enhancement on contrast-enhanced scans, and demonstrates heterogeneous increases in FDG uptake.

Results of routine thoracic radiographic examinations rarely impact the plan to proceed with anesthetic procedures in dogs with presumed acute intervertebral disc herniation.

Acute thoracolumbar intervertebral disc herniation (IVDH) constitutes an emergency because associated neurological clinical signs can be progressive, with prognosis dependent on preoperative presence of deep pain perception. Pre-anesthetic thoracic radiographs are routinely performed to evaluate for potential pathology that could result in increased risk or change in overall prognosis. However, due to the emergent nature of this disease, the weight of thoracic radiographic findings on treatment plans for these dogs in unknown. The objective of this study was to investigate the clinical benefit of thoracic radiographs prior to advanced spinal imaging and surgery for acute non-ambulatory dogs with suspected T3-L3 myelopathy. Consecutive dogs presented with presumed acute IVDH between July 2020-July 2022 were identified, and medical records were reviewed retrospectively. Age, body weight, and alteration of treatment plan were compared between the dogs with positive thoracic pathology identified on thoracic radiographs and those with negative thoracic pathology. Only 2/105 dogs had their diagnostic and treatment plans changed due to thoracic radiographic findings. Dogs with radiographic pathology diagnosed (2/16) were more likely to have their treatment plans changed than dogs with no radiographic pathology (0/89) (p = 0.014). The odds of radiographic thoracic pathology were 4.6 times higher in dogs aged 12 years or older [OR 4.6 (95%CI 1.2-17, p = 0.026]. Performing routine thoracic radiography prior to advanced (anesthetized) diagnostic spinal imaging in presumed IVDH cases rarely resulted in a change to the treatment plan, thought eh practice may be clinically relevant in dogs 12 years of age or older.

Prediction of Survival in the Elderly Patients with Glioblastoma using Cumulative Inflammatory Markers Score

Abstract Objectives This retrospective study aimed to explore the prognostic effect of cumulative score based on neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and fibrinogen in older adults diagnosed with glioblastoma (GBM). Design Retrospective study. Setting Huashan Hospital. Participants Patients aged over 60 years and diagnosed with GBM between 2010 and 2017. Main Outcome Measures Results of preoperative routine biochemistry and coagulation blood examinations were reviewed from medical records. Overall survival (OS) was considered a period from first resection surgery until death. Progression-free survival (PFS) was considered a period from initial operation until the date of tumor progression demonstrated in brain magnetic resonance imaging or death from any cause. If no event occurred, the last follow-up appointment was the end of the observation for OS or PFS. The Kaplan–Meier method was used to evaluate survival curves, and prognostic factors were analyzed by the Cox proportional hazards model. Results A total of 289 patients were included. Patients with higher levels of fibrinogen, NLR, and PLR had significantly shorter median OS (p = 0.001, p = 0.016, and p = 0.002, respectively) and PFS (p = 0.004, p = 0.022, and p = 0.009, respectively) compared with those with lower levels. Multivariate analyses showed a significant association between higher F-NLR-PLR score and reduced OS (adjusted hazard ratios [aHRs]: 1.356, 95% confidence interval [CI] 1.009–1.822 for scores 1–2 compared with 0; 5.974, 95% CI 2.811–12.698 for score 3 compared with 0). Similarly, a significant association between higher F-NLR-PLR score and reduced PFS was observed (aHR: 1.428, 95% CI 1.066–1.912 for scores 1–2 compared with 0; aHR: 2.860, 95% CI 1.315–6.223 for score 3 compared with 0). Conclusion Higher F-NLR-PLR score is associated with reduced OS and PFS in older adults with GBM, which helps identify patients at high risk and guide the individualized treatment in clinical practice.

SCABIOSIS AND TOXOCARIOSIS IN A LOCAL DOG

A two months old dog, Local Breed male, weighing 1.5 kg with clinical symptoms of crusting on both ears, lichenification on both hind legs and neck, erythema on the alopecia, hair loss almost all over the body, scaly skin (scale), abscesses on both hind legs and enlarged abdomen. The dog looks very dangerous with pruritus 9/10. The purpose of this article is to discuss the management of scabiosis and toxocariosis cases with therapy using sarolaner and pyrantel. On superficial examination of skin scraping, trichogram and skin tape, Sarcoptes scabiei mites were found. On stool examination using the original method, Toxocara canis worm eggs were found. Routine hematological examination results showed that the case dog had hyperchromic microcytic anemia, leukocytosis and lymphocytosis. Based on anamnesis, physical examination and support, the dog case was diagnosed with scabiosis and toxocariosis. Treatment was carried out by administering sarolaner (Simparica®), pyrantel (Drontal®), chlorpheniramine maleate, fish oil, Curcuma (herbafit®), vitamin B complex (Caviplex®), and bathing with antikeratolytic shampoo (sebazole®). On the 30th day after treatment showed improvement with no lesions found on the skin and hair growing properly. In addition, the stomach is not enlarged and the oral mucosa is normal, which is pink. Based on anamnesis, clinical examination, and clinical symptoms, the case dog was diagnosed with scabiosis and toxocariosis with a fausta prognosis. Therapy with sarolaner and pyrantel gives good results. Owners are advised to maintain the cleanliness of the environment where the dog is, and provide food and drink clean food. Giving pyrantel worm medicine to other animals needs to be done so that toxocariosis does not infect other animal cases. Apart from that, other animals need to be given sarolaner medicine so that scabiosis is no longer contagious. In addition, owners are advised to always wash their hands after contact with other animals so as not to transmit the disease to animal cases and are advised not to release animals again.

Prevalence and influential factors of isolated hepatitis B core antibody positivity in a Chinese adult population

Isolated anti-HBc (IAHBc) is defined by the presence of anti-HBc in the absence of HBsAg and hepatitis B surface antibody (anti-HBs). IAHBc is of great clinical significance as a specific pattern of HBV infection, but IAHBc has not been fully clarified. This study aimed to explore the prevalence and influential factors of IAHBc from routine examination results of inpatients.A total of 61,247 individuals were included in the study, with a median age of 55 years (range: 43–68), and a male-to-female ratio of 0.90:1. The prevalence of current HBV infection (HBsAg positive) was 6.82%, while the prevalence of previous HBV infection (HBsAg negative but anti-HBc positive) was 48.63%. The prevalence of IAHBc was 12.31%. Among them, the rates for males were 7.10%, 52.16%, and 13.70%, respectively, which were significantly higher than the rates for females at 6.56%, 45.45%, and 11.06% (P < 0.05). The prevalence rates mentioned above were significantly reduced after vaccination (P < 0.05). The prevalence of IAHBc increases with age, rising from 0.23% in the age group of 15–29 years to 13.57% in individuals aged 80 and above. After the age of 50, the prevalence of IAHBc closely parallels the previous infection rate but shows no significant association with the current infection rate (P > 0.05). Among IAHBc individuals, approximately 33.83% tested positive for anti-HBe, and their anti-HBc absorbance values were significantly higher compared to anti-HBe negative individuals (7.08 and 5.31, P < 0.01). The prevalence of anti-HBe positivity among IAHBc individuals does not vary with changes in the previous infection rate and age (P > 0.05).

The Diagnostic Potential of the L Score for ABO Hemolytic Disease of the Newborn: Insights from a Cross-Sectional Study

PurposeThis study aimed to evaluate the diagnostic efficacy of the L score, a novel scoring system, in distinguishing between ABO hemolytic disease of the newborn (ABO-HDN) and non-hemolytic disease of newborn hyperbilirubinemia (NHDNH).MethodsA cross-sectional prospective study was conducted to assess the effectiveness of the L score in distinguishing between ABO-HDN (n = 118) and NHDNH (n = 213). Blood routine examination results were collected, and relevant statistical analyses were performed to identify clinically significant parameters. Binary logistic regression analysis was employed to assess the relationship between the L score and the development of these conditions, considering relevant variables.ResultsOur study identified the red blood cell count, mean corpuscular volume, red blood cell distribution width—coefficient of variation, and red blood cell distribution width—standard deviation as independent risk factors for distinguishing ABO-HDN from other high bilirubinemia conditions (P < 0.001). The L score demonstrated superior predictive performance for ABO-HDN, exhibiting an area under the curve (AUC) of 0.746, with an optimal cutoff value of − 3.0816. The RBC-L score exhibited superior predictive performance (z: 5.596, P < 0.0001) compared to the single-factor RBC indicator, indicating its efficacy in accurately identifying the desired outcome.ConclusionThe L score represents a valuable tool for predicting neonatal hyperbilirubinemia and hemolytic disease, facilitating differentiation, and guiding early intervention for improved outcomes. Further research is warranted to validate and expand the applicability of the L score in clinical practice.

“Only the Sufferer Knows”: Long-term problems and experiences of kidney transplant recipients: A phenomenological study

BackgroundUnderstanding the experiences of kidney transplant recipients is important to ensure their inclusion in key strategies and future planning of renal services. AimTo explore the long-term problems and experiences of adults who have had a kidney transplant due to end-stage kidney disease after discharge. MethodsThis was a descriptive phenomenological study. The data were collected through in-depth interviews. A personal information form and semi-structured interview guide were used in the interviews. The interviews were recorded using a voice recorder and conducted by adhering to the semi-structured interview guide. When data saturation was reached and no new information emerged, data collection was terminated after the 17th participant, whose responses were found to be repetitive. In this study, a descriptive phenomenological design and thematic analysis approach based on Husserl’s philosophy was used. The data analysis process was carried out independently by all researchers. Throughout this process, the researchers held regular meetings to discuss and compare the emerging findings. Individual, semi-structured, in-depth interviews were transcribed verbatim. The findings were analysed using Colaizzi's seven-step analysis. ResultsIn total, 17 kidney transplant recipients (five women and 12 men) were included in the study. In the data analysis process, three main themes and 10 sub-themes were identified. Main themes: (i) problems experienced by kidney transplant recipients, (ii) inevitable fears, and (iii) situations that only individuals with kidney transplants can understand — “Only the Sufferer Knows”. Sub-themes: management of drug therapy, role–relationship management, permanent treatment responsibilities and burden, fear of rejection, fear of returning to dialysis treatment, pervasive fear of routine examination results, significance of kidney transplant surgery, sense of gratitude towards the donor, being ‘sick’ in the eyes of others, and not being understood by others. ConclusionThis study provides new insights into understanding the lives of post-transplant kidney transplant recipients. Renal healthcare professionals should focus on the psychological impact as much as the biological impact on recipients of kidney transplants.

Evolving a Model for Cochlear Implant Outcome.

Cochlear implantation is an efficient treatment for postlingually deafened adults who do not benefit sufficiently from acoustic amplification. Implantation is indicated when it can be foreseen that speech recognition with a cochlear implant (CI) is superior to that with a hearing aid. Especially for subjects with residual speech recognition, it is desirable to predict CI outcome on the basis of preoperative audiological tests. The purpose of the study was to extend and refine a previously developed model for CI outcome prediction for subjects with preoperative word recognition to include subjects with no residual hearing by incorporating additional results of routine examinations. By introducing the duration of unaided hearing loss (DuHL), the median absolute error (MAE) of the prediction was reduced. While for subjects with preoperative speech recognition, the model modification did not change the MAE, for subjects with no residual speech recognition before surgery, the MAE decreased from 23.7% with the previous model to 17.2% with the extended model. Prediction of word recognition with CI is possible within clinically relevant limits. Outcome prediction is particularly important for preoperative counseling and in CI aftercare to support systematic monitoring of CI fitting.

UNILATERAL RHINITIS AND HEMOBARTONELLA FELIS INFECTION IN DOMESTIC CATS WITH A HISTORY OF CHRONIC KIDNEY DISEASE

Rhinitis is an inflammation of the mucous membranes of the nasal cavity, which can arise from a number of intranasal or systemic disorders. Hemobartonella felis is a gram-negative intracellular bacterium that attacks cat's erythrocytes. This treatment aims to discuss diagnostic and treatment techniques for rhinitis and Hemobartonellosis infection in cat with kidney failure. The animal is a local cat with a history of kidney failure, female, 6 years old and weighing 2.7 kg. The owner came with complaints of a serous discharge in the right nasal cavity and occasional sneezing. The cat has decreased appetite since 6 months and looks weak. Clinical examination showed swelling of the right mandibular lymph nodes and inspection of the oral cavity found gingivitis, tartar, and halitosis. Routine hematological examination results showed that the cat had macrocytic hypochromic anemia accompanied by thrombocytopenia. Examination of the blood smear preparations found coccoid formations with short chains or rod formations in the cat's erythrocytes which indicated a positive result for Hemobartonella felis. Case animal was diagnosed with unilateral rhinitis and Hemobartonella felis infection. The therapy given consisted of Doxycycline antibiotics at a dose of 10 mg/kg once a day for 14 days, anti-inflammatory Dexamethasone at a dose of 0.5 mg once a day for 5 days and Sangobion as a supportive therapy 1 capsule once a day for 10 days. The therapy given helps to improve the condition of the case animal. General check up of health conditions is required during the maintenance period.

Clinical analysis of sixty-nine children with anomalous aortic origin of the coronary artery

We aimed to analyse the clinical characteristics of children with different types of anomalous aortic origin of the coronary artery (AAOCA) at different ages, and to discuss the factors related to myocardial ischaemia. In this retrospective study, we included 69 children diagnosed with AAOCA using CT coronary angiography; we classified the participants based on the type of AAOCA, age, and high-risk anatomy. The clinical characteristics of the different AAOCA types and age groups were compared, and the correlation between manifestations and high-risk anatomy was analysed. Anomalous origin of the left coronary artery from the right coronary artery sinus, anomalous origin of the right coronary artery from the left coronary artery sinus, and a coronary artery origin without coronary sinuses was found in 10 (14.5%), 57 (82.6%), 2 (2.9%) patients, respectively. There were no significant differences in sex, clinical manifestations, percentage of positive myocardial injury markers, electrocardiogram, transthoracic echocardiography, or proportion of high-risk anatomy among the groups with different AAOCA types. According to age group, the proportion of asymptomatic infants and pre-schoolers was the highest (p < 0.001). Forty-three patients (62.3%) had high-risk anatomy and were more likely to present with severe symptoms and cardiac syncope (p < 0.05). Conclusion: There were no significant differences in the proportions of high-risk anatomy and clinical characteristics among children with different AAOCA types. We found a relation between the severity of AAOCA clinical symptoms and anatomical risk.What is Known:• Clinical symptoms in children with AAOCA are varied and the results of routine cardiological examinations lack specificity.• High-risk anatomical features, exercise, cardiac symptoms, and ALCA are risk factors for the occurrence of SCD in patients with AAOCA.What is New:• Compared the clinical characteristics of different types of AAOCA and ages.• Analysed the correlation between symptoms and high-risk anatomical features.

Assessment of the Epidemic Process of COVID-19 Manifestations and the Epidemiological Efficacy of the Vaccine Gam-Covid-Vac Among Employees of a Multi-Speciality Medical Organization

Relevance. Employees of medical organizations (MO), by virtue of their official duties, are at the group risk of morbidity of a new coronavirus infection.Aim. Evaluation of the manifestations of the COVID-19 epidemic process and the epidemiological effectiveness of the use of the Gam-Covid-Vac vaccine among employees of a multy-speciality medical organization.Materials and methods. The incidence of COVID-19 among the staff of MO was studied for the period from March 2020 to December 2021. The latent epidemic process was evaluated avccording to the results of routine serological examination of employees for antibodies of classes IgM and IgG to SARS-CoV-2. The epidemiological effectiveness of immunoprevention was determined by comparing the incidence with the coverage of employees who has a completed course of vaccination.Results. During the study period, 47.7% of employees of medical organizations had a clinically apparent form of COVID-19. In addition, according to the results of a routine serological examination, it turned out that 46.5% of employees had a subclinical form of infection. An increased level of morbidity was observed in the autumn period. The incidence of medical workers was in 2.2 times higher than other employees, medical staff of the hospital – in 1.7 times higher than polyclinics. The epidemic process was more intense in the group of nurses than doctors and junior medical staff. The epidemiological efficacy of the Gam-Covid-Vac during the first months of using vaccine has been established. Vaccinated people have a more mild course of COVID-19 in comparison with unvaccinated people.Conclusion. In this way, the main manifestations of the COVID-19 epidemic process in employees of a multy-speciality medical organization were identified and the epidemiological effectiveness of the Gam-Covid-Vac vaccine in the first months of using vaccine was established

ANALYSIS OF FASTING ON BLOOD TESTS

As a procedure to obtain information with bodily fluid samples, a laboratory test is complementary to a diagnostic assessment. One parameter of laboratory tests is hematology, for which a blood profile is used to evaluate clinical conditions and provide diagnostic information about hematological abnormalities, prognosis, and response treatment. Nowadays, accessibility to laboratory tests has been made easier with medical checkups integrated into the Occupational Health and Safety program, which a company is obliged to have to check on its employees’ health status. Our study analyzed the results of fasting blood tests on the employees of The College of Health Sciences of Al-Irsyad Al-Islamiyyah Cilacap. This College is known for the practice of Islamic and religious values, including fasting, among its employees. Taking the employees as a population and samples, the research included variables of levels of erythrocytes, leukocytes, hemoglobin, and hematocrit in the descriptive analysis method. We gained eleven respondents, 8 males, and 3 females, and used a cluster sampling method with appropriate criteria. The data of obtained samples were analyzed and processed based on the interpretation of the results. The results of routine blood examinations on fasting showed 90.90% normal hemoglobin levels and 72.70% normal hematocrit levels. Conclusively, our results of the blood profile examination on hemoglobin and hematocrit in fasting people showed that fasting is metabolically healthy and produces no indication of anemia.

Analysis of prognostic factors and establishment of prognostic model for primary mediastinal germ cell tumors: a case controlled study.

The overall prognosis of primary mediastinal germ cell tumors (PMGCTs) is poor and the associated prognostic factors are not fully understood. Our goal was to investigate the prognostic factors of PMGCTs and to develop a validated prognostic prediction model. A total of 114 PMGCTs with specific pathological types were included in this study. Clinicopathological characteristics of nonseminomatous PMGCTs and mediastinal seminomas were compared using the χ2 or Fisher's exact test. Independent prognostic factors of nonseminomatous PMGCTs screened using the univariate and multivariate Cox regression analysis were then used to generate a nomogram. The predictive performance of the nomogram was evaluated using the concordance index, decision curve, and the area under the receiver operating characteristic curve (AUC) and validated by bootstrap resampling. The Kaplan-Meier curves of independent prognostic factors were analyzed. This study included 71 cases of nonseminomatous PMGCTs and 43 cases of mediastinal seminomas. The 3-year overall survival rates for nonseminomatous PMGCTs and mediastinal seminomas patients were 54.5 and 97.4%, respectively. The overall survival prognostic nomogram for nonseminomatous PMGCTs was established by integrating independent prognostic factors, including the Moran-Suster stage, white blood cell, hemoglobin, and platelet-lymphocyte ratio. The nomogram demonstrated good performance with a concordance index of 0.760 and the 1-year and 3-year AUC values of 0.821 and 0.833, respectively. These values were better than those of the Moran-Suster stage system. The bootstrap validation had an AUC of 0.820 (0.724-0.915) and showed a well-fitting calibration curve. Besides, patients with mediastinal seminomas showed favorable clinical outcomes and all the nine patients received neoadjuvant therapy and postoperative surgery achieved pathological complete response. A nomogram based on staging and blood routine examination results was established to accurately and consistently predict the prognosis of patients with nonseminomatous PMGCTs.

Overview of Routine Hematology Results in COVID-19 Patients at UKRIDA Hospital in 2021

Coronavirus Disease 2019, more commonly known as COVID-19, is an infectious disease that has a contagious nature, the etiologic cause is Severe Acute Respiratory Syndrome Coronavirus 2. Several clinical manifestations that commonly occur when infected with COVID-19 include acute respiratory system disorders, for example cough, fever and shortness of breath. on March 12 2020, WHO confirmed that COVID-19 was a pandemic case. This research was conducted in a cross-sectional descriptive manner, using consecutive sampling with a total sample of 110 patients, using secondary data in the form of medical records. It was concluded that the results of routine hematological examinations (hemoglobin, hematocrit, erythrocytes, leukocytes, platelets) were mostly normal, this was because the patients in the study sample were patients who had recently been infected with the Covid-19 virus, and the medical records taken were the results of a hematological examination. the first time when I entered the hospital. On examination of hemoglobin found 22 patients (20%) decreased. On leukocyte examination, it was found that 28 patients (25.5%) experienced a decrease in platelets, 15 patients (13.6%) decreased.

Correlations of joint detection of 22 vaginal microbes with routine examination results of vaginal secretions and assisted reproductive outcomes

The correlations of joint detection of 22 vaginal microbes with routine examination results of vaginal secretions and assisted reproductive outcomes were investigated. There were 37 samples with abnormal vaginal microecology in 107 vaginal secretion samples. The top 5 detection rates of microorganisms were Ureaplasma urealyticum (73.83%), Prevotella sp. (70.09%), Gardnerella vaginalis (53.27%), L. crispatus (52.34%) and L. inerts (51.40%). When the levels of Bacillus and hydrogen peroxide in vaginal secretions decreased or pH increased, the abnormal rates of vaginal microecology increased significantly (P < 0.01). The clinical pregnancy rate (53.66%, 22/41) in the women with normal vaginal microecology was higher than that (37.5%, 9/24) with abnormal vaginal microecology. In conclusions, the joint detection of 22 vaginal microbes can quickly and effectively determine whether the vaginal microecology is normal or not. The evaluation of vaginal microecology may be valuable in predicting the assisted reproductive outcomes of infertile women.

Management of Bleeding due to Vitamin K Deficiency in Neonates: A Case Report

Vitamin K deficiency bleeding (VKDB) is an uncommon and life-threatening bleeding condition in infancy. This study aimed to present a case of bleeding due to vitamin K deficiency in neonates. A 7-day-old male infant presented to an emergency department (ED) following bleeding from an umbilical stump for 3 hours upon arrival at the hospital. On examination of the general condition, the patient is in good health, responsive, conscious and active tonus. Physical examination revealed there were no petechiae, purpura, or ecchymoses. On the abdomen region, there was minimal bleeding in an umbilical area with periumbilical hemorrhagic crusting. Intense palpation revealed mild tenderness near the periumbilical area. When the umbilical area was palpated deeply, a thin, watery blood fluid oozed out. The results of routine blood laboratory examinations showed within normal limits (Hb 12 g/dL, leukocytes 6.9x103/μL, and platelets 150x10³/μL). This patient was diagnosed with bleeding due to vitamin K deficiency. The management given is in the form of a subcutaneous injection of vitamin K 10 mg for 3 days. After administration of the third injection, the patient's condition improves, and the umbilical hemorrhage subsides.

Online Buying and Selling Application of Sacrificial Animals Using Scrum Method During the Covid-19 Pandemic

Since the pandemic entered Indonesia, many people have switched to the online buying and selling transaction process.The Covid-19 pandemic has had a very significant impact on the transaction process which was previously carriedout traditionally, especially for the sale and purchase of sacrificial animals. The process of selling sacrificial animalsis still done traditionally by ordering directly by phone and coming directly to the cattle pen for the sacrificial animalsdesired by consumers. To deal with these problems, an alternative solution is needed that can create a informationsystem solution as a medium for selling livestock online. This online sacrificial animals buying and selling applicationwill be developed using the SCRUM method. When conditions change like this during the Covid-19 pandemic, SCRUMis suitable for developing applications. This is because Scrum is an iterative method that is included in the Agilemethod in how to manage and run an application development project. The features or menus that will be built in thisapplication including Master Data Management, and get Reports on all Transactions periodically, Shipping / DeliveryTransactions, notifications for Animal Husbandry Services. However, due to the conditions of the Covid-19 pandemic,there are several features that will be added, namely the Results of Routine Examination of the Condition of SacrificialAnimals for the last 1 month which will continue to be updated by livestock owners, and Description of PhysicalCondition of Sacrificial Animals which includes Species, Skin Color, Weight (Kg ), Height (cm). The results of theBlack Box Testing show that all functions have run well with a percentage of successful functional running of 97.3%.Keywords: Application, Online Buying and Selling, Sacrificial Animals, Scrum Method.