Results Of Analyses Research Articles

Construction of 3D network aluminum sludge-based hydrogel beads: combination of macroization, amino functionalization, and resource utilization.

An aluminum sludge-based composite material was constructed against the problems of phosphorus pollution and the waste of aluminum sludge resources. Utilizing metal Ce doping and hydrogel microbeads with pore preparation, the adsorption performance of the original sludge was improved. Meanwhile, the macroscopic body was constructed, and on this basis, polyethyleneimine (PEI) was introduced to complete the amino functionalization further to enhance the adsorption of phosphorus by the adsorbent, and NH-CeAIS-10 microbeads were successfully prepared. In adsorption, microbeads with larger specific surface area and richer functional groups are better choice compared to original sludge. The results of SEM, BET, FT-IR, and XPS analyses indicate that the adsorption of phosphorus by the microbeads is mainly achieved through electrostatic interactions, ligand exchange, and the formation of inner-sphere complexes. According to the Langmuir model, the maximum phosphorus adsorption capacity of NH-CeAIS-10 was 29.56mgg-1, which was four times higher compared to native aluminum sludge. This also confirms the significant enhancement of phosphorus adsorption through the modification of aluminum sludge. Besides, in dynamic adsorption column experiments, the material exhibited up to 99% removal in simulated wastewater for up to 30days, demonstrating the great adsorption potential of NH-CeAIS-10 in engineering applications.

Abstract C038: Interim pharmacodynamic analyses of mitazalimab in combination with FOLFIRINOX in first-line metastatic pancreatic ductal adenocarcinoma (mPDAC) identify CD4 effector T cells as a correlate of treatment outcomes

Abstract CD40 agonists are being investigated in combination with standard of care chemotherapy as an approach to trigger anti-tumor immunity and improve outcomes for patients with cancer. Preclinical models suggest the importance of appropriate sequencing of chemotherapy and a CD40 agonist. Herein, we report interim pharmacodynamic analyses and their association with outcomes from a Phase 2 study evaluating mitazalimab, a CD40 agonist, in combination with modified (m) FOLFIRINOX chemotherapy as first line therapy in patients with metastatic pancreatic ductal adenocarcinoma (mPDAC). Patients with newly diagnosed mPDAC were treated with mitazalimab on day 1 prior to beginning an every 2-week regimen on day 8 of mFOLFIRINOX followed by mitazalimab 48 hours later. Tumor response was determined by RECIST v1.1 imaging. Peripheral blood was analyzed for selected cytokines and chemokines and by flow cytometry to assess changes in leukocyte subsets including B cells, monocytes, T cells, and NK cells. The neutrophil-to-lymphocyte ratio was determined from complete blood counts. The analysis included 23 patients with associated response data and indicated a mitazalimab-specific contribution to tumor response. Mitazalimab triggered an expected immune response characterized by transient cytokine (IL-8, IP-10, MCP-1, and MIP1b) release and B cell depletion. Chemotherapy provoked a reduction in classical monocytes and proliferating CD4+ T cells. Both mitazalimab and chemotherapy caused a transient reduction in circulating dendritic cells and NK cells. Tumor response was associated with an expansion in the frequency of effector CD4 T cells (p < 0.0001) at day 8 after receiving mitazalimab but did not correlate with neutrophil-to-lymphocyte ratio (p = 0.851). In conclusion, mitazalimab and FOLFIRINOX modulate immune responses in patients with mPDAC in different ways. Interim results of pharmacological analyses identify CD4 effector T cells as a correlate of treatment outcomes suggesting the contribution of mitazalimab to tumor responses. Citation Format: Max M. Wattenberg, Karin Enell Smith, Yago Pico de Coana, David Gomez Jimenez, Malin Carlsson, Sumeet Vijay Ambarkhane, Peter Ellmark, Gregory L. Beatty. Interim pharmacodynamic analyses of mitazalimab in combination with FOLFIRINOX in first-line metastatic pancreatic ductal adenocarcinoma (mPDAC) identify CD4 effector T cells as a correlate of treatment outcomes [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Pancreatic Cancer; 2023 Sep 27-30; Boston, Massachusetts. Philadelphia (PA): AACR; Cancer Res 2024;84(2 Suppl):Abstract nr C038.

Late age at first birth is a protective factor for oesophageal cancer and gastro-oesophageal reflux: the evidence from the genetic study.

The primary objective of this research endeavor was to examine the underlying genetic causality between the age at first birth (AFB) and four prevalent esophageal diseases, namely oesophageal obstruction (OO), oesophageal varices (OV), gastro-oesophageal reflux (GOR), and oesophageal cancer (OC). We conducted a two-sample Mendelian randomization (MR) analysis to examine the causal association between AFB and four prevalent esophageal disorders. We employed eight distinct MR analysis techniques to evaluate causal relationships, encompassing random-effects inverse variance weighted (IVW), MR Egger, weighted median, simple mode, weighted mode, maximum likelihood, penalized weighted median, and fixed-effects IVW. The random-effects IVW method served as the primary approach for our analysis. Furthermore, we executed several sensitivity analyses to assess the robustness of the genetic causal inferences. The random-effects IVW analysis revealed a significant negative genetic causal association between AFB and both GOR (P < 0.001, Odds Ratio [OR] 95% Confidence Interval [CI] = 0.882 [0.828-0.940]) and OC (P < 0.001, OR 95% CI = 0.998 [0.998-0.999]). Conversely, there was insufficient evidence support to substantiate a genetic causal link between AFB and OO (P = 0.399, OR 95% CI = 0.873 [0.637-1.197]) or OV (P = 0.881, OR 95% CI = 0.978 [0.727-1.314]). The results of sensitivity analyses underscore the robustness and reliability of our MR analysis. The findings of this investigation substantiate the notion that elevated AFB confers a protective effect against GOR and OC. In addition, no causative association was discerned between AFB and OO or OV at the genetic level.

Spatial Distribution and Accessibility Analysis of Primary School Facilities in Mega Cities: A Case Study of Chengdu

High-quality and equitable primary education services promote the building of a harmonious socialist society and are an important basis for improving people’s quality of life and promoting high-quality and sustainable regional development. Here, we take Chengdu City as a test area, integrate data from multiple sources, use the random forest model to simulate the distribution data of primary school-age children in Chengdu City in 2020, and use the kernel density estimation method and the multi-traffic mode two-stage floating catchment area method to measure the spatial distribution characteristics and accessibility of primary school educational facilities in Chengdu City and combine the imbalance index and spatial autocorrelation analysis, examination of the equalization of the distribution of primary school educational facilities, and the correlation between school-age population and accessibility. The results show that in the past decade, the population of Chengdu has grown rapidly, and the number of primary school-age children has also been increasing. The overall distribution of primary school-age children in Chengdu presents a decentralized pattern of “one point with multiple cores”, with the population decreasing from the center to the periphery, and the population distribution dominates the spatial distribution of primary school facilities, which also highlights the imbalance in the construction of primary school facilities to some extent (S = 0.257), which was mainly manifested by the fact that the central-eastern part of the city has more primary school facilities, while the western part has fewer. In addition, the results of both accessibility and autocorrelation analyses show that the overall accessibility of the central circle of Chengdu was high, while the accessibility of the second and third circles was at a lower level and below, with very obvious cross-regional and cross-circle differences. This study can not only provide more accurate recommendations for the allocation of educational facilities but also serve as a reference for evaluating the spatial equity of other public services in the city.

Seismic strengthening of isolated RC framed structures through orthogonal steel exoskeleton: Bidirectional non-linear analyses

The catastrophic effects of recent earthquakes around the world have highlighted the vulnerability of civil buildings; in Europe, most of the existing buildings were built after World War II and since they have largely exceeded their useful life, are characterized by a high vulnerability linked both to the durability of the materials and to the lack of seismic provisions in design. The current trend in constructional field is to design interventions aimed at the seismic and energy improvement of these structures jointly, in order to achieve a sustainable and integrated retrofit intervention. To this aim, the use of 2D orthogonal steel exoskeletons for the seismic strengthening represents a suitable solution, providing at the same time an enhancement in thermal insulation, through a ‘double-skin’ solution. Due to the growing interest in the design of steel exoskeleton interventions, a detailed dynamic characterization of this external strengthening solution is needed. In this framework, the present work, aims to investigate the effectiveness of the investigated strengthening solution by means of bi-directional non-linear dynamic analyses. Extensive numerical analysis was carried out. The selected case-study is an Italian existing pre-1980 s RC frame building located in Mugnano di Napoli (NA, Italy), which is a medium-high seismic hazard site in Italy (peak ground acceleration equal to 0.156 g). Two alternative strengthening solutions were numerically investigated by means of non-linear time-history and incremental dynamic analyses performed applying fifteen bidirectional ground motions on three-dimensional numerical models. The results of the dynamic non-linear analyses allow to quantify more appropriate performance indicators, both in terms of expected demand and overall collapse capacity, useful for the development of optimization strategy and design of exoskeleton system. The results highlighted that both the investigated strengthening scenarios allow to increase the stiffness of the existing building and allow it to meet the current code safety requirements reducing also the residual inter-storey drift. The obtained results are not strictly related to the investigated case study, but can be extended to all the structures retrofitted that follows the presented design procedure.

Role of citric acid in enhancing aromatic hydrogenation performance in Ni-HPW/SiO2 catalysts

In this study, we utilized a sol-gel method to prepare Ni-HPW/SiO2 catalysts with varying citric acid (CA):Ni molar ratios ranging from 0 to 5.0. The catalysts demonstrated different levels of activity in the hydrogenation of ethylbenzene, which correlated to their respective CA:Ni ratios. Introducing citric acid in the preparation process of nickel-based catalysts yielded mesopore formation and reduced NiO particle size, producing a more uniform NiO dispersion on SiO2, which was confirmed by BET, XRD, and TEM analyses. The results of H2-TPR, H2-TPD, NH3-TPD, and Py-IR analyses suggested that the addition of citric acid augmented the interaction between phosphotungstic acid (HPW), Ni species, and SiO2. Citric acid improved Ni species and HPW dispersion, fostering increased HPW-Ni species formation. This, in turn, generated additional electron-deficient Ni ions, stemming from semi-reduced HPW-Ni species, which acted as Lewis acid sites, enhancing Ni+ species effectiveness in activating aromatic rings. The better-dispersed, non-HPW-bound NiO, which, upon complete reduction to Ni, led to a substantial rise in H2 bond cleavage, thereby facilitating more hydrogen spillover. Overall, the infusion of citric acid into fabricating Ni-HPW/SiO2 catalysts sparked a significant surge in their aromatic hydrogenation ability.

Financial inclusion empowering sustainable technologies: Insights into the E-7 economies from COP28 perspectives

COP28 highlighted the essential interplay between financial inclusion and sustainable technologies in driving effective climate action. This strategy underscores the importance of aligning financial systems with technological innovations to mitigate environmental pollution and achieve net-zero carbon emissions. The limited availability of funds, however, continues to hinder growth and widespread adaptation of technological advancement, and a significant research gap pertaining to the financial inclusion still remains. In order to address this gap; this study investigates the role of financial inclusion and technological advancement in the context of environmental pollution for the E-7 countries. In this regard, the results of the cointegration analyses show evidence of long-term relationships between TA, Financial inclusion, and environmental degradation. Moreover, the results of our study also show that financial inclusion positively influences carbon emissions. However, financial inclusion is a substantive moderator that tends to intensify the negative association between TA and CO2 emissions. In addition to this, our results also show that energy productivity and technological advancement significantly reduce CO2 emissions and enhance the quality of the environment. Our study also uncovers the positive correlations between GDP, trade openness and carbon emissions, thus signaling towards their adverse effect on the environment.

Identification of cuproptosis-related diagnostic biomarkers in idiopathic pulmonary fibrosis.

Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal lung disease with clinical and pathological heterogeneity. Recent studies have identified cuproptosis as a novel cell death mechanism. However, the role of cuproptosis-related genes in the pathogenesis of IPF is still unclear. Two IPF datasets of the Gene Expression Omnibus database were studied. Mann-Whitney U test, correlation analysis, functional enrichment analyses, single-sample gene set enrichment analysis, CIBERSORT, unsupervised clustering, weighted gene co-expression network analysis, and receiver operating characteristic curve analysis were used to conduct our research. The dysregulated cuproptosis-related genes and immune responses were identified between IPF patients and controls. Two cuproptosis-related molecular clusters were established in IPF, the high immune score group (C1) and the low immune score group (C2). Significant heterogeneity in immunity between clusters was revealed by functional analyses results. The module genes with the strongest correlation to the 2 clusters were identified by weighted gene co-expression network analysis results. Seven hub genes were found using the Cytoscape software. Ultimately, 2 validated diagnostic biomarkers of IPF, CDKN2A and NEDD4, were obtained. Subsequently, the results were validated in GSE47460. Our investigation illustrates that CDKN2A and NEDD4 may be valid biomarkers that were useful for IPF diagnosis and copper-related clustering.

Petrophysical Analyses of Rock Construction Materials from a Roman Rural Settlement in Podšilo Bay on Rab Island (North-East Adriatic, Croatia).

This article presents the results of petrophysical analyses of limestones and sandstones used for the construction of the wall structures of a Roman rural settlement located in Podšilo Bay on Rab Island (Croatia). An on-site analysis of the walls indicated the use of different lithotypes, which is an uncommon case in the area. So far, no petrophysical properties of the applied materials have been tested, and their provenance has not been specified. The aim of this research was to determine their usability as construction materials in an attempt to determine the possible reasons behind the usage of multiple lithotypes and their suitability as building materials. The following procedure was used to address these issues: (1) determination of the petrographic characteristics of the rocks, (2) performance of tests to characterise the mechanical properties in a complex stress state of uniaxial tension followed by uniaxial and triaxial compression, and, finally, (3) determination of the internal structure of the rocks using methods based on X-ray imaging. Multi-proxy characteristics of the materials including numerous observations and methods were performed: optical microscopy used to characterise rock petrography and mineralogy, scanning electron microscopy (SEM) coupled with EDS, as well as grinding tests; furthermore, mechanical properties were determined on cylindrical samples in accordance with European standards. X-ray microtomography using the XμCT method enabled microscopic observations and determination of the orientation of discontinuities and the rock structure. The performed analyses allowed us to distinguish three lithological types of sandstone and two types of limestone among the examined stone blocks. Stone blocks of fine- and medium-grained sandstone with carbonate binders, as well as sparitic limestone and mudstone with calcite veins, were used to build the studied structures. The analysed blocks showed traces of partial edge processing. Despite the defects in the material structure identified using XμCT, all the types of rock were characterised by high or very high strength. High values of longitudinal wave velocity confirmed the good quality of the material. These results contribute to a better understanding of the construction process and the related technological choices, and they provide the first dataset which can be used for the reconstruction of the building's original appearance in the future.

A novel end-to-end chromosome classification approach using deep neural network with triple attention mechanism

The automated karyotyping analysis serves as a crucial tool in diagnosing genetic disorders stemming from chromosome abnormalities. Chromosome classification, a pivotal step in this diagnostic process encounters challenges due to the non-rigid structure and unpredictable curved shapes of chromosomes. Extracting features with discriminative power for enhanced classification accuracy becomes difficult, impacting the practical results of karyotyping analyses in clinical applications. In this paper, we propose a novel end-to-end chromosome classification approach based on deep neural network with a triple attention mechanism (TAM). The approach unfolds in three stages. Firstly, deep CNNs and modified Multiscale Feature Fusion (MFF) blocks collaborate to generate informative multi-scale global representations. In the second stage, to uncover finer features that enhance chromosome differentiation, TAM is devised. It learns channel-wise, spatial-wise and point-wise attention feature maps fully extracting valuable information from multi-scale global features. Finally, a probability-weighted prediction scheme is incorporated to synthetically estimate classification branches at all scales, facilitating type identification. The evaluation results, based on a clinical G-band chromosome dataset comprising 52,808 single chromosome images, indicate that the proposed chromosome classification approach attains an impressive accuracy of 99.4%. In conclusion, our method exhibits strong robustness and high stability in effectively classifying multiple types of chromosomes.

JPEG-compatible Joint Image Compression and Encryption Algorithm with File Size Preservation

Joint image compression and encryption algorithms are intensively investigated due to their powerful capability of simultaneous image data compression and sensitive information protection. Unfortunately, most of the existing algorithms suffered from either poor compression efficiency or weak encryption strength, making them vulnerable to cryptanalysis. To address these limitations, we propose a chaos-based JPEG-compatible joint image compression and encryption algorithm. We separate the luminance and chrominance coefficients to preserve file size and encrypt the discrete cosine transform (DCT) coefficients in parallel. The proposed inter-block DC encryption strategy achieves high encryption intensity based on the permutation-substitution structure. In addition, we apply both inter- and intra-block permutations to AC coefficients and strengthen the encryption using an inter-block substitution for non-zero AC coefficients. The results of security and performance analyses demonstrate that the proposed algorithm offers robust encryption of image data while maintaining compression efficiency for real-time transmission.

Insight on the interaction between soybean protein isolate and ionic/non-ionic polysaccharides: Structural analysis, oil-water interface properties investigation and double emulsion formation

In this study, hydroxypropyl methylcellulose (HPMC) and xanthan gum (XG) were utilized to enhance the interfacial activity of soybean protein isolate (SPI) by forming complexes with SPI, respectively. Meanwhile, SPI-HPMC and SPI-XG were used to construct a double emulsion to investigate the effects of the interaction of ionic/nonionic polysaccharides with SPI on the performances of the double emulsion. The results of multispectral analyses showed that both HPMC and XG induced structural changes in SPI, thereby exposing a large number of hydrophobic groups. Moreover, molecular simulation results showed high stability of the complexes formed by SPI with HPMC and XG. The results of molecular simulations showed that the complexes formed by SPI with HPMC and XG were highly stable. Furthermore, the ability of SPI to reduce interfacial tension at the oil-water interface and its emulsifying ability was enhanced by the formation of complexes with polysaccharides, which also improved the physicochemical properties of the double emulsion stabilized by the SPI-polysaccharide complex. When the phase ratio (W1/O:W2) was 4:6 and the polysaccharide addition was 0.4%, the double emulsion showed the smallest particle size, the highest encapsulation efficiency and the best stability. Th in vitro digestion models demonstrated that the SPI-XG double emulsion could effectively protect the encapsulated bioactives during the digestion process and increase their bioaccessibility to 70.86%. In summary, this study was beneficial in corroborating the ability of ionic/non-ionic polysaccharides in improving the interfacial properties of SPI and improving the performance of the double emulsion.

Non-alcoholic fatty liver disease increases the risk of cardiovascular disease in young adults and children: a systematic review and meta-analysis of cohort studies.

It is uncertain if there is a link between non-alcoholic fatty liver disease (NAFLD) and cardiovascular diseases (CVD) in young adults and children. To evaluate the potential link between these two conditions, we conducted a systematic review and meta-analysis of cohort studies. A comprehensive search was conducted in PubMed, Web of Science and Embase in order to locate all relevant cohort studies published until August 2023. Random effects meta-analyses were conducted using the generic inverse variance method, with additional subgroup and sensitivity analyses. The Newcastle-Ottawa Scale was employed to evaluate the methodological quality. Four cohort studies (eleven datasets) involving 10,668,189 participants were included in this meta-analysis. This meta-analysis demonstrated that NAFLD increases the risk of CVD in young adults and children (HR = 1.63, 95% CI: 1.46-1.82, P < 0.00001). Further subgroup analyses showed that individuals with NAFLD were at a heightened risk of coronary heart disease (CHD) (HR = 3.10, 95% CI: 2.01-4.77, P < 0.00001), myocardial infarction (MI) (HR = 1.69, 95% CI: 1.61-1.78, P < 0.00001), atrial fibrillation (AF) (HR = 2.00, 95% CI: 1.12-3.57, P = 0.02), congestive heart failure (CHF) (HR = 3.89, 95% CI: 1.20-12.61, P = 0.02), and stroke (HR = 1.47, 95% CI: 1.39-1.55, P < 0.00001). The results of subgroup analyses based on the study location, NAFLD definition, and follow-up time also showed consistency with the overall results. Sensitivity analyses showed that our results were robust. All of the included studies were judged to be of medium to high quality. Current evidence reveals that NAFLD is linked to an increased risk of major CVD (including CHD, MI, AF, CHF and stroke) in young adults and children. Further research is needed to strengthen this association and provide stronger evidence for primary prevention of CVD in young adults and children with NAFLD. https://www.crd.york.ac.uk/PROSPERO/, PROSPERO registration number: CRD42023457817.

The interaction effects of light and noise on the acoustic parameters of underwater vocalisations of wild spotted seals (Phoca largha) in Liaodong Bay, China1

Some marine mammals can adjust acoustic parameters of their sound signals in response to changes in sunlight and ambient noise. Evidence was found in several toothed whales; however, relatively little research has focused on true seals. Spotted seals (Phoca largha) are highly vociferous during the breeding season. Four major underwater call types, drums, growls, knocks, and sweeps, have been identified in the acoustic repertoire of wild spotted seals in Liaodong Bay, China. This study investigated the potential effects of light (day and night) and noise (105–135 dB re 1 μPa, broadband 50–8,000 Hz) conditions on the centroid frequency (CF), root-mean-square bandwidth (BW), duration (DU), and received level (RL) parameters of their underwater vocalisations using a series of generalised linear models (GLMs). Results showed that there were significant interaction effects of light and noise on knock CFs and the BWs for all call types. Specifically, knock CFs decreased and BWs for all call types became narrower when noise increased at night but kept stable during daytime. No significant interactions were found in other acoustic parameters. However, the results of univariate GLM analyses showed that the spotted seals could lengthen their vocalisations at night and increase the vocalisation RLs in response to higher noise levels (the Lambard effect), regardless of call type. To our knowledge, this is the first study to demonstrate that the spotted seals, a poorly studied species in Chinese waters, have some capabilities to adjust their vocalisations in response to changes in light and noise conditions. The results are helpful to understand how seals interact with their habitats and useful when developing mitigation strategies to minimise disturbance of light and noise from human activities in Liaodong Bay, China.

Biotransformation and detoxification of tetrabromobisphenol A by white-rot fungus Phanerochaete sordida YK-624

The bulky phenolic compound tetrabromobisphenol A (TBBPA) is a brominated flame retardant used in a wide range of products; however, it diffuses into the environment, and has been reported to have toxic effects. Although it is well-known that white-rot fungi degrade TBBPA through ligninolytic enzymes, no other metabolic enzymes have yet been identified, and the toxicity of the reaction products and their risks have not yet been examined. We found that the white-rot fungus Phanerochaete sordida YK-624 converted TBBPA to TBBPA-O-β-D-glucopyranoside when grown under non-ligninolytic-enzyme-producing conditions. The metabolite showed less cytotoxicity and mitochondrial toxicity than TBBPA in neuroblastoma cells. From molecular biological and genetic engineering experiments, two P. sordida glycosyltransferases (PsGT1c and PsGT1e) that catalyze the glycosylation of TBBPA were newly identified; these enzymes showed dramatically different glycosylation activities for TBBPA and bisphenol A. The results of computational analyses indicated that the difference in substrate specificity is likely due to differences in the structure of the substrate-binding pocket. It appears that P. sordida YK-624 takes up TBBPA, and reduces its cytotoxicity via these glycosyltransferases.

Experimental investigation of thermal performance evaluation and thermodynamic analysis of domestic box type solar cooker with inclined aperture area

In this paper, the thermal performances of a box solar cooker were rated with F1 and F2 parameters as per Mullick’s procedure [1], the efficiency of the cooker was evaluated using energy and exergy analysis. The solar cooker was designed with an inclined aperture area, realized and experimentally tested at Applied Research Unit on Renewable Energies of Ghardaïa (32.39 °N, 3.78 °E, 463 m), Algeria. The developed cooker is equipped with one reflector mirror on its lid. The experiments have been carried out with the cooker empty, filled with one or two liters of water from 08:00 to 15:00 solar time, the thermal profiles of various components of the cooker were assessed at Ghardaïa site Sahara climatic conditions on clear days in both winter and summer months. According to the values of two figures of merits suggested by International Standards and evaluated by the experimental studies, the configuration with an inclined aperture area of the cooker resulted in good cooking performances, especially in winter when the sun’s elevation is low; keeping the realized cooker performances in range of the international standard tests. The results of this study indicate also that the average daily energy and exergy efficiency of the realized cooker was 16.2 % and 0.90 % respectively, during the experimental period, which indicate a significant difference between the results of energy and exergy analyses. Thus developing such a box solar cooker can improve the efficiency of the design and use a position of the system to prepare food in a shorter time.

Risk Factors Analysis and Pathogen Distribution of Urinary Tract Infection in Patients Undergoing Cutaneous Ureterostomy After Radical Cystectomy for Bladder Cancer.

Postoperative urinary tract infection is a common complication that not only significantly prolongs the hospital stay and amplifies the economic burden on patients, but also affects their quality of life and prognosis. This study aimed to investigate risk factors and distribution of pathogenic bacteria in urinary tract infections among bladder cancer patients who underwent cutaneous ureterostomy following radical cystectomy. A total of 137 bladder cancer patients, who underwent cutaneous ureterostomy after radical cystectomy at our hospital from November 2018 to October 2022, were enrolled in this retrospective study. Univariate and multivariate logistic regression analyses were employed to investigate the risk factors associated with postoperative urinary tract infection and the distribution of pathogenic bacteria among the infected patients. The results of both univariate and multivariate analyses confirmed that age, proficiency in ostomy knowledge, frequency of ureteral stent tube replacement, ureteral stent tube dislodgement, urine immersion at the outer end of the ureteral stent tube, and the interval of ostomy bag replacement were independent risk factors for urinary tract infection after radical cystectomy and cutaneous ureterostomy in bladder cancer patients. A total of 55 pathogenic bacteria were isolated from 52 patients with infections. Predominantly, these were gram-negative bacteria (34 strains, 61.8%), with Proteus mirabilis having the highest proportion. Urinary tract infections after radical cystectomy and cutaneous ureterostomy predominantly involve gram-negative bacteria. This is correlated with factors such as the age of bladder cancer patients, the level of nursing education, the duration of ureteral stent tubes and ostomy bag usage, as well as issues related to impaired urine drainage.

Kinetic analysis and mechanism of nitrate, calcium, and cadmium removal using the newly isolated Pseudomonas sp. LYF26

The co-existence of heavy metals and nitrate (NO3−-N) pollutants in wastewater has been a persistent global concern for a long time. A strain LYF26, which can remove NO3−-N, calcium (Ca(II)), and cadmium (Cd(II)) simultaneously, was isolated to explore the properties and mechanisms of synergistic contaminants removal. Different conditions (Cd(II) and Ca(II) concentrations and pH) were optimized by Zero-, Half-, and First-order kinetic analyses to explore the environmental parameters for the optimal effect of strain LYF26. Results of the kinetic analyses revealed that the optimal culture conditions for strain LYF26 were pH of 6.5, Cd(II) and Ca(II) concentrations of 3.00 and 180.00 mg L−1, accompanied by Ca(II), Cd(II), and NO3−-N efficiencies of 53.10%, 90.03%, and 91.45%, respectively. The removal mechanisms of Cd(II) using strain LYF26 as a nucleation template were identified as biomineralization, lattice substitution, and co-precipitation. The differences and changes of dissolved organic matter during metabolism were analyzed and the results demonstrated that besides the involvement of extracellular polymeric substances in the precipitation of Cd(II) and Ca(II), the high content of humic acid-like species revealed a remarkable contribution to the denitrification process. This study is hopeful to contribute a theory for further developing microbially induced calcium precipitation used to treat complex polluted wastewater.

Cybersecurity Awareness among Secondary School Students Post Covid-19 Pandemic

The COVID-19 pandemic has significantly transformed the landscape of education delivery, leading to a surge in online teaching and learning methods, and consequently, increased Internet usage among students. These circumstances have created an environment that make them vulnerable to cybercrime due to the lack of cybersecurity awareness. This paper proposes a cyber-awareness program aimed at increasing students' knowledge of cybersecurity and their ability to manage the risk of cyber threats. The methodology employed consists of four phases: Initiation and Planning, Development of Modules, Implementation, and Evaluation. The results of descriptive and statistical analyses show an increase in participants' awareness of cyber security threats and risks following their participation in the program. In conclusion, this program has successfully achieved its objectives of enhancing cyber security awareness and promoting safe internet usage among participants. These findings suggest that similar programs can be utilized to increase cybersecurity awareness and promote safe internet usage among the students.

Studying on mineralogical and petrological characteristics of Gara Djebilet oolitic iron ore, Tindouf (Algeria)

Demand for iron ore worldwide has been steadily increasing which leads to the extraction of iron ore deposits with more complex mineralogies and higher levels of silicon and phosphorus impurities. This is the case in Algeria with the iron ore deposit of Gara Djebilet, Tindouf; where it has recently been exploited to ensure the sufficiency of iron ore required to produce iron and steel products. This deposit has remained unexploited for several decades due to inadequate knowledge of its mineralogy, treatment, and economic assessments. The aim of this study is to find out the microstructure, chemical composition, and mineralogical distribution of valuable minerals and impurities, to understand the efficient processing methods for this specific iron ore. The characterization of representative ironstone samples taken from the studied area was carried out using optical microscopy, X-ray fluorescence spectrometer (XRF), petrographic microscope, X-ray diffractometer (XRD), and scanning electron microscope (SEM) coupled with energy dispersive X-ray spectroscopy (EDS). The results of the mineralogical analyses confirmed that it is an oolitic fine-grained ore consisting of gangue minerals principally composed of quartz, apatite, and iron-rich concentric cored structures. Chemical analyses of the ore indicate that it contains 56.58 wt% Fe with 7.98 wt% SiO2, 7.09 wt% Al2O3, and minor amounts of P2O5, CaO, MgO, and TiO2 compounds. The phosphorus associated was present in both ooids and groundmass, indicating that the ore has a complex texture with very rich and diverse mineralogy. For that, two potential scenarios were proposed for processing of the studied iron ore.