Restricted Pool Research Articles

Pyritic stromatolites from the Paleoarchean Dresser Formation, Pilbara Craton: Resolving biogenicity and hydrothermally influenced ecosystem dynamics.

This study investigates the paleobiological significance of pyritic stromatolites from the 3.48 billion-year-old Dresser Formation, Pilbara Craton. By combining paleoenvironmental analyses with observations from well-preserved stromatolites in newly obtained drill cores, the research reveals stratiform and columnar to domal pyritic structures with wavy to wrinkly laminations and crest thickening, hosted within facies variably influenced by syn-depositional hydrothermal activity. The columnar and domal stromatolites occur in strata with clearly distinguishable primary depositional textures. Mineralogical variability and fine-scale interference textures between the microbialites and the enclosing sediment highlight interplays between microbial and depositional processes. The stromatolites consist of organomineralization - nanoporous pyrite and microspherulitic barite - hosting significant thermally mature organic matter (OM). This includes filamentous organic microstructures encased within nanoporous pyrite, resembling the extracellular polymeric substance (EPS) of microbes. These findings imply biogenicity and support the activity of microbial life in a volcano-sedimentary environment with hydrothermal activity and evaporative cycles. Coupled changes in stromatolite morphology and host facies suggest growth in diverse niches, from dynamic, hydrothermally influenced shallow-water environments to restricted brine pools strongly enriched in from seawater and hydrothermal activity. These observations, along with S stable isotope data indicating influence by S metabolisms, and accumulations of biologically significant metals and metalloids (Ni and As) within the microbialites, help constrain microbial processes. Columnar to domal stromatolites in dynamic, hydrothermally influenced shallow water deposits likely formed by microbial communities dominated by phototrophs. Stratiform pyritic structures within barite-rich strata may reflect the prevalence of chemotrophs near hydrothermal venting, where hydrothermal activity and microbial processes influenced barite precipitation. Rapid pyrite precipitation, a putative taphonomic process for preserving microbial remnants, is attributed to microbial sulfate reduction and reduced S sourced from hydrothermal activity. In conclusion, this research underscores the biogenicity of the Dresser stromatolites and advances our understanding of microbial ecosystems in Earth's early history.

Mantis shrimp appendages-inspired Ag-CuO contact: Synergistically enhancing the erosion and impact resistance via gradient-layered scatter-island/chain-skeleton structure

Materials degradation induced by repeated arc erosion and impact poses a considerable challenge for Ag-based contacts. Fortunately, nature has evolved efficient strategies to construct complex gradient-layered structure that exhibit exceptional erosion and impact resistance. Drawing inspiration by the mantis shrimp appendages, a chain-like CuO nanofibre-reinforced Ag-CuO contact material with a gradient-layered scatter-island/chain-skeleton structure was designed and fabricated. Employing a combined experimental and simulation approach, the evolution of the microstructure during the erosion process was investigated. Results indicated that the interaction between the mantis shrimp appendage-like structure and the chain-like CuO nanofibres effectively suppressed the formation of cracks and pores on the contact surface. Particularly, the scatter-island layer not only played a crucial role in maintaining the viscosity of the molten pool and establishing stable electrical and thermal pathways, but also functioned as a “supplement” to continuously supply a steady source of Ag onto the molten pool surface, effectively promoting CuO skeleton restructuring. Meanwhile, the CuO skeleton diffusive-continuous restructuring of the chain-skeleton layer restricted molten pool flow, significantly enhancing contact erosion resistance. Furthermore, the strong synergistic effect between the gradient-layered structures dispersed stress and deformation concentration on the contact surface. It effectively delayed the defects formation and suppressed interfacial debonding, thereby endowing the contact with excellent impact resistance. Our work presents a fascinated route for designing novel biomimetic Ag-based contacts.

Factors influencing competence development of lecturers in library and information science in Vietnam

PurposeThis article aims to identify and analyze the factors influencing lecturers’ need to develop their competencies in the field of Library and Information Science (LIS), with a specific focus on the University of Social Sciences and Humanities, Vietnam National University – Ho Chi Minh City (USSH, VNU-HCM).Design/methodology/approachThe study employs an interview method to collect data from lecturers on LIS at USSH, VNU-HCM. Interviews were transcribed verbatim and analyzed according to the qualitative methodology of content analysis.FindingsThe results indicate that individual, organizational, disciplinary and professional factors significantly impact the competence development of LIS lecturers. Detailed information about the components of each factor, particularly the roles of professional awareness, career goals, motivation and time management skills is also provided.Research limitations/implicationsThis article is limited by a restricted interview participant pool and a sole focus on one university. The study recommends expanding the inclusion of participants to ensure a more diverse perspective and enhance the applicability of the findings.Practical implicationsThe research findings offer valuable insights for designing policies, training programs and work environments for lecturers within educational and training institutions in the LIS field.Originality/valueThis study provides a fresh perspective on the challenges and opportunities for developing human resources in the LIS sector in Vietnam.

Deciphering environmental forcings in the distribution of meiofauna and nematodes in mangroves of the Atlantic-Caribbean-East Pacific and Indo-West Pacific regions

Mangroves develop under environmental conditions and anthropogenic pressures whose impact on benthic meiofauna remains poorly understood. It is unclear how meiofauna communities are structured according to local sedimentary conditions. This study was designed to characterize the community structure of meiofauna and nematodes (dominant taxa) and the associated environmental forcings in intertidal mangrove sediments from Mayotte (Indo-West-Pacific), Martinique and Guadeloupe (Caribbean). Sediment cores were sampled at the end of the dry season at low tide on adult mangrove stands with similar immersion time. In each sediment layer, we analyzed redox potential, pH, porewater salinity, grain size, organic matter, metals, organic contaminants, prokaryotes and meiofauna. Our results show that sediments far from cities and agricultural fields trapped site-specific contaminants due to local water transport processes. Some metals, PAHs or pesticides exceeded toxicity thresholds in most of the studied stations, thus being harmful to benthic fauna. The sedimentary environment acts as a filter selecting specific meiofauna communities at station scale only in the Caribbean. In Mayotte, horizontal homogeneity contrasts with vertical heterogeneity of the sedimentary environment and the meiofauna. Nematode genera showed particular distribution patterns horizontally and vertically, suggesting the presence of sediment patches suitable for a restricted pool of genera on each island. Results in the Caribbean are consistent with nested diversity patterns due to environmental filtering. Conversely, horizontal homogeneity at Mayotte would reflect greater dispersal between stations or more spatially homogeneous anthropogenic pressures. The nematode genera present at depth may not be the most specialized, but the most versatile, capable of thriving in different conditions. Terschellingia and Daptonema showed contrasted responses to environmental forcing, likely due to their versatility, while Desmodora showed uniform responses between study areas, except when toxicity thresholds were exceeded. Our results emphasize that a given genus of nematode may respond differently to sedimentary conditions depending on sites.

Angioleiomyoma: An Update with a 142-Case Series.

Angioleiomyomas are uncommon, noncancerous, smooth muscle tumors that primarily arise from blood vessels. Previous studies have yielded limited data due to the lack of interdisciplinary approaches or restricted patient pools. This study aims to provide a comprehensive analysis of angioleiomyomas, including the demographic, clinical, radiological, and histopathological features, with a large number of patients. Conducted as a retrospective investigation at a single center from January 2005 to June 2023, this study involved 142 patients. Relevant information was extracted from electronic medical records, covering clinical, radiological, histological, and demographic details. Angioleiomyomas mostly occurred at age 59 (1-87), predominately affect females (53%) and commonly arise in subcutaneous tissue (85%) and the lower limbs (76%). MRI findings revealed characteristic signals, with a high prevalence of the solid histologic type (65%), often displaying a reticular sign. Smooth muscle Actin was universally present (n = 95/95), while Desmin and Caldesmon showed positive expression in 83% (n = 71/85) and 98% (n = 92/94) of cases, respectively. This study presents an updated and comprehensive analysis of angioleiomyomas. Typically appearing as well-defined nodules in the extremities, these tumors can be effectively diagnosed using MRI, though histopathological analysis is generally essential for confirmation. Treatment primarily involves straightforward excision, with notable low complication and recurrence rates.

Effect of masker selection schemes on the perceived affective quality of soundscapes: A pilot study

The perceived quality of a given soundscape may be improved by adding "pleasant" sounds via a speaker or electroacoustic system to the existing soundscape as maskers. Ideally, the choice of sounds should be automated to minimize the time and labor required to maintain such a system. Hence, we investigate the effect of several automated masker selection schemes on improving the ISO Pleasantness of urban soundscapes in a controlled laboratory environment. An ambisonic recording and 360-degree video of a location exposed to traffic noise was made and reproduced via a stacked planar speaker array and virtual reality headset. Simultaneously, maskers from a fixed candidate pool were overlaid on the reproduced soundscape in one of the following methods: random selection across the entire pool, fixed selection based on results in the literature, random selection across a restricted pool based on model-agnostic analysis of a pre-existing dataset, and random selection across a restricted pool based on a deep neural network trained on a pre-existing dataset. We also investigated a control condition with no added maskers, and evaluated the results based on changes in ISO Pleasantness ratings given by participants to the soundscape under each masker selection scheme, relative to the control condition.

Revisiting the roles of cAMP signalling in the progression of prostate cancer.

Prostate cancer is one of the most common cancers in men and one of the top causes of death in men worldwide. Development and function of both normal prostate cells and early-stage prostate cancer cells are dependent on the cross-talk between androgen signalling systems and a variety of other transduction pathways which drive differentiation of these cells towards castration-resistance. One such signalling pathway is the ubiquitous cAMP signalling axis which functions to activate spatially restricted pools of cAMP effectors such as protein kinase A (PKA). The importance of both PKA and cAMP in the development of prostate cancer, and their interactions with the androgen receptor, were the focus of a review by Merkle and Hoffmann in 2010. In this updated review, we revisit this topic with analysis of current PKA-related prostate cancer literature and introduce novel information on the relevance of another cAMP effector, the exchange protein directly activated by cAMP (EPAC).

Developmental emergence of cortical neurogliaform cell diversity.

GABAergic interneurons are key regulators of cortical circuit function. Among the dozens of reported transcriptionally distinct subtypes of cortical interneurons, neurogliaform cells (NGC) are unique: recruited by long-range excitatory inputs, source of slow cortical inhibition and able to modulate the activity of large neuronal populations. Despite their functional relevance, the developmental emergence and diversity of NGCs remains unclear. Here, by combining single-cell transcriptomics, genetic fate-mapping, electrophysiological and morphological characterization, we reveal that discrete molecular subtypes of NGCs, with distinctive anatomical and molecular profiles populate the neocortex. Furthermore, we show that NGC subtypes emerge gradually through development, as incipient discriminant molecular signatures are apparent in Preoptic Area (POA) born NGC precursors. By identifying NGC developmentally-conserved transcriptional programs, we report that the transcription factor Tox2 constitutes an identity hallmark across NGC subtypes. Using CRISPR-Cas9-mediated genetic loss-of-function, we show that Tox2 is critical for NGC development: POA-born cells lacking Tox2 fail to differentiate into NGCs. Together, these results reveal that NGCs are born from a spatially restricted pool of Tox2+ POA precursors, after which intra-type diverging molecular programs are gradually acquired post-mitotically and result in functionally and molecularly discrete NGC cortical subtypes.

Striatal mGlu5-mediated synaptic plasticity is independently regulated by location-specific receptor pools and divergent signaling pathways

Metabotropic glutamate receptor 5 (mGlu5) is widely expressed throughout the central nervous system and is involved in neuronal function, synaptic transmission, and a number of neuropsychiatric disorders such as depression, anxiety, and autism. Recent work from this lab showed that mGlu5 is one of a growing number of G protein-coupled receptors that can signal from intracellular membranes where it drives unique signaling pathways, including upregulation of extracellular signal-regulated kinase (ERK1/2), ETS transcription factor Elk-1, and activity-regulated cytoskeleton-associated protein (Arc). To determine the roles of cell surface mGlu5 as well as the intracellular receptor in a well-known mGlu5 synaptic plasticity model such as long-term depression, we used pharmacological isolation and genetic and physiological approaches to analyze spatially restricted pools of mGlu5 in striatal cultures and slice preparations. Here we show that both intracellular and cell surface receptors activate the phosphatidylinositol-3-kinase-protein kinase B-mammalian target of rapamycin (PI3K/AKT/mTOR) pathway, whereas only intracellular mGlu5 activates protein phosphatase 2 and leads to fragile X mental retardation protein degradation and de novo protein synthesis followed by a protein synthesis-dependent increase in Arc and post-synaptic density protein 95. However, both cell surface and intracellular mGlu5 activation lead to α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor GluA2 internalization and chemically induced long-term depression albeit via different signaling mechanisms. These data underscore the importance of intracellular mGlu5 in the cascade of events associated with sustained synaptic transmission in the striatum.

The legacy of human use in Amazonian palm communities along environmental and accessibility gradients

AbstractAimPalms are iconic and dominant elements of neotropical forests. In the Amazon region, palms have been used and managed by humans for food, material, medicine and other purposes for millennia. It is, however, debated to what extent the structure of modern palm communities reflects long‐term human modification. Here, we investigate the complex interplay of ecological and societal factors that influence the distributions of both human‐used and non‐used palms in western Amazonia.LocationAmazonia.Time periodPresent.Major taxa studiedPalms (Arecaceae).MethodsWe used Bayesian hierarchical joint species distribution models to predict the distributions and environmental niche dimensions of 78 western Amazonian species, and to explore their relationships with their diversity of human uses and with specific uses (food, construction and medicine). The models were parameterized with a comprehensive set of field‐ and satellite‐derived environmental predictors.ResultsOur results suggest that a combination of ecological and anthropogenic factors drive the present‐day distributions of Amazonian palms. The modelled ecological niches of the species revealed use‐related species‐sorting along soil, climatic, accessibility and drainage gradients. We found peaks in the proportions of useful palms and their diversity of uses in fertile soils, close to rivers, and on floodplains. These are habitats favourable for human settlement, although they harbour naturally restricted palm species pools. We also found a negative correlation between predicted palm species richness and number of human uses across western Amazonia.Main conclusionsSoil characteristics, accessibility, and species pool size all contribute to defining palm–human relationships. At the basin scale, the signature of human use on palm communities was predicted to be stronger in the species‐poor south‐west than in central‐western Amazonia. Overall, we conclude that environmental conditions have influenced modern Amazonian palm distributions both directly and indirectly, by regulating human settlement patterns and natural resource use over extended time periods.

Lessons from the biosphere for the anthroposphere: Analysis of recycling structures of conservational measures

This paper analyzes cyclic and transferring maximal pathways in some natural and human-built process structures to show how the essential features of recycling based conservation are embedded in natural systems, and how these lessons can be implemented in human-built systems, consciously. Having studied an example biosystem and ecosystem, it was found that i) local open transferring routes result in cycles at the system level; ii) the diversified species are built from a restricted pool of fundamental components; iii) assembly and disassembly are coordinated by symmetrical structural patterns; iv) recycling is supported by storage, resulting in average composition. Conscious applications of these features are discussed for the carbon emission-free hydrogen energy cycle; for a published case study on grocery bag recycling and for possible assembly-disassembly logistics of electronic waste reprocessing. The household waste-related example also illustrates how process net structure can be applied for the generation of the respective dynamic model.

MIRACLE: MInd ReAding CLassification Engine.

Brain-computer interfaces (BCIs) have revolutionized the way humans interact with machines, particularly for patients with severe motor impairments. EEG-based BCIs have limited functionality due to the restricted pool of stimuli that they can distinguish, while those elaborating event-related potentials up to now employ paradigms that require the patient's perception of the eliciting stimulus. In this work, we propose MIRACLE: a novel BCI system that combines functional data analysis and machine-learning techniques to decode patients' minds from the elicited potentials. MIRACLE relies on a hierarchical ensemble classifier recognizing 10 different semantic categories of imagined stimuli. We validated MIRACLE on an extensive dataset collected from 20 volunteers, with both imagined and perceived stimuli, to compare the system performance on the two. Furthermore, we quantify the importance of each EEG channel in the decision-making process of the classifier, which can help reduce the number of electrodes required for data acquisition, enhancing patients' comfort.

The Current State of Sickle Cell Disease Management: A Concise Review on Stem Cell Transplant as a Cure

Treatment of sickle cell disease remains largely palliative. While it may improve the quality of life, persons with sickle cell disease still suffer from extreme sickling crises, end-organ damage, and reduced life expectancy. Increasing studies have led to the identification and advancement of stem cell transplant and gene therapy as potential curative strategies for sickle cell disease. However, there have been various factors that have hindered their clinical application. Stem cell transplantation, the more propitious of the two, is limited by restricted transplant donor pool, transplant complications, and selection criteria. The current paper reviewed the literature on sickle cell disease, current treatment options, and more particularly on the progress of stem cell transplants. It outlined various challenges of stem cell transplant and proposed ways to increase the donor pool using alternative strategies and modifications of regimen conditioning with minimal transplant-related toxicities and associated complications. Keywords: Cord blood, Haploidentical, Sickle cell disease, Stem cell transplantation

Reconstructing late Miocene depositional environments in the central High Plains, USA: Lithofacies and architectural elements of the Ogallala Formation

The late Miocene Ogallala Formation hosts much of the High Plains aquifer of North America, though it is poorly studied in the central High Plains region where only a small fraction of its total thickness is exposed. One exception is in western Kansas, where up to 40 m of the Ogallala Formation crop out along the bluffs of Ladder Creek Canyon. These deposits consist of stacked and laterally extensive, multistory channel bodies dominated by sandy bedforms with little or no intervening floodplain mudstone. We interpret each ~2–4 m-thick story as a sandstone bed that formed during a single depositional episode and infilled a broad and relatively shallow braided river channel. Each story preserves elements suggesting high discharge conditions, followed by low discharge or the complete abandonment of the river channel after active streamflow either migrated away from the study area or ceased regionally for a relatively long period of time. High discharge conditions are characterized by channel-filling, current-formed transverse bars, gravel-rich longitudinal bars, and sand sheets deposited while the channel was largely flooded and nearly the entire riverbed mobilized. Low discharge conditions are indicated by lens-shaped, trough cross-stratified channels scoured into previously deposited bars. Channel-fill deposits were formed when the remaining flow was confined to one or more narrow streams that braided across the exposed channel belt. Persistent low discharge conditions are suggested by channel-belt-wide subaerial exposure of bar surfaces for a period of time sufficient to promote colonization by soil-burrowing organisms and moderate soil development. Abandonment of the broader channel belt is indicated by advanced calcretization within a story and the presence of aerially restricted and fossiliferous mud-filled channel pools where the final repositories of surface water attracted local paleofauna before infilling or drying completely. The depositional environment of the Ogallala Formation in the study area is most similar to “Platte type” fluvial systems characterized as shallow, perennial, and sand-dominated braided rivers. Comparisons with previously studied localities in the northern and southern High Plains reveal differences in fluvial style, sediment source areas, cut-and-fill geometries, and eolian input.

Developmental trajectories of thalamic progenitors revealed by single-cell transcriptome profiling and Shh perturbation.

The thalamus is the principal information hub of the vertebrate brain, with essential roles in sensory and motor information processing, attention, and memory. The complex array of thalamic nuclei develops from a restricted pool of neural progenitors. We apply longitudinal single-cell RNA sequencing and regional abrogation of Sonic hedgehog (Shh) to map the developmental trajectories of thalamic progenitors, intermediate progenitors, and post-mitotic neurons as they coalesce into distinct thalamic nuclei. These data reveal that the complex architecture of the thalamus is established early during embryonic brain development through the coordinated action of four cell differentiation lineages derived from Shh-dependent and -independent progenitors. We systematically characterize the gene expression programs that define these thalamic lineages across time and demonstrate how their disruption upon Shh depletion causes pronounced locomotor impairment resembling infantile Parkinson's disease. These results reveal key principles of thalamic development and provide mechanistic insights into neurodevelopmental disorders resulting from thalamic dysfunction.

Validating a Novel Device to Improve Skin Color Matching for Face Transplants.

A prospective study of 100 individuals was conducted. All participants were photographed and scanned with the Nix Color Sensor. Sixty pairwise comparisons were randomly generated. Skin color analysis was performed using photographs and the Nix Color Sensor. Delta E2000 values were compared to mean evaluator ratings using a Spearman correlation analysis. One hundred patients were included. A Spearman's correlation demonstrated a strong inverse correlation between Delta E2000 values and the mean evaluator ratings. The higher the mean evaluator rating for likeness, the lower the delta. A correlation coefficient of -0.850 demonstrates a statistically significant relationship (P < 0.01). When the Delta E2000 rises above 5 there is a significant drop in the mean evaluator ratings. As mean evaluator ratings of 5 and above would be considered adequate for face transplant amongst most plastic surgeons, an E2000 value of 5 or lower should be targeted when matching donors with recipients for face transplant. The Nix Color Sensor positively correlates to the plastic surgeon's perception of skin color and can serve as an adjunct in donor selection for facial VCAs.

FEMALE SEX IS NOT ASSOCIATED WITH INCREASED SURVIVAL AFTER NON-TRAUMATIC OUT OF HOSPITAL CARDIAC ARREST: A SYSTEMATIC REVIEW AND META-ANALYSIS

FEMALE SEX IS NOT ASSOCIATED WITH INCREASED SURVIVAL AFTER NON-TRAUMATIC OUT OF HOSPITAL CARDIAC ARREST: A SYSTEMATIC REVIEW AND META-ANALYSIS

A pooled Bayes test of independence using restricted pooling model for contingency tables from small areas

A pooled Bayes test of independence using restricted pooling model for contingency tables from small areas

Epitope mapping of severe acute respiratory syndrome coronavirus 2 neutralizing receptor binding domain-specific monoclonal antibodies.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the outbreak led to the coronavirus disease 2019 (COVID-19) pandemic. Receptor binding domain (RBD) of spike (S) protein of SARS-CoV-2 is considered as a major target for immunotherapy and vaccine design. Here, we generated and characterized a panel of anti-RBD monoclonal antibodies (MAbs) isolated from eukaryotic recombinant RBD-immunized mice by hybridoma technology. Epitope mapping was performed using a panel of 20-mer overlapping peptides spanning the entire sequence of the RBD protein from wild-type (WT) Wuhan strain by enzyme-linked immunosorbent assay (ELISA). Several hybridomas showed reactivity toward restricted RBD peptide pools by Pepscan analysis, with more focus on peptides encompassing aa 76–110 and 136–155. However, our MAbs with potent neutralizing activity which block SARS-CoV-2 spike pseudovirus as well as the WT virus entry into angiotensin-converting enzyme-2 (ACE2) expressing HEK293T cells showed no reactivity against these peptides. These findings, largely supported by the Western blotting results suggest that the neutralizing MAbs recognize mainly conformational epitopes. Moreover, our neutralizing MAbs recognized the variants of concern (VOC) currently in circulation, including alpha, beta, gamma, and delta by ELISA, and neutralized alpha and omicron variants at different levels by conventional virus neutralization test (CVNT). While the neutralization of MAbs to the alpha variant showed no substantial difference as compared with the WT virus, their neutralizing activity was lower on omicron variant, suggesting the refractory effect of mutations in emerging variants against this group of neutralizing MAbs. Also, the binding reactivity of our MAbs to delta variant showed a modest decline by ELISA, implying that our MAbs are insensitive to the substitutions in the RBD of delta variant. Our data provide important information for understanding the immunogenicity of RBD, and the potential application of the novel neutralizing MAbs for passive immunotherapy of SARS-CoV-2 infection.

Dbx1 controls the development of astrocytes of the intermediate spinal cord by modulating Notch signaling.

Significant progress has been made in elucidating the basic principles that govern neuronal specification in the developing central nervous system. In contrast, much less is known about the origin of astrocytic diversity. Here, we demonstrate that a restricted pool of progenitors in the mouse spinal cord, expressing the transcription factor Dbx1, produces a subset of astrocytes, in addition to interneurons. Ventral p0-derived astrocytes (vA0 cells) exclusively populate intermediate regions of spinal cord with extraordinary precision. The postnatal vA0 population comprises gray matter protoplasmic and white matter fibrous astrocytes and a group of cells with strict radial morphology contacting the pia. We identified that vA0 cells in the lateral funiculus are distinguished by the expression of reelin and Kcnmb4. We show that Dbx1 mutants have an increased number of vA0 cells at the expense of p0-derived interneurons. Manipulation of the Notch pathway, together with the alteration in their ligands seen in Dbx1 knockouts, suggest that Dbx1 controls neuron-glial balance by modulating Notch-dependent cell interactions. In summary, this study highlights that restricted progenitors in the dorsal-ventral neural tube produce region-specific astrocytic subgroups and that progenitor transcriptional programs highly influence glial fate and are instrumental in creating astrocyte diversity.