Response Of Tomato Plants Research Articles

Fumonisin B1-Induced Oxidative Burst Perturbed Photosynthetic Activity and Affected Antioxidant Enzymatic Response in Tomato Plants in Ethylene-Dependent Manner

Fumonisin B1 (FB1) is a harmful mycotoxin produced by Fusarium species, which results in oxidative stress leading to cell death in plants. FB1 perturbs the metabolism of sphingolipids and causes growth and yield reduction. This study was conducted to assess the role of ethylene in the production and metabolism of reactive oxygen species in the leaves of wild type (WT) and ethylene receptor mutant Never ripe (Nr) tomato and to elucidate the FB1-induced phytotoxic effects on the photosynthetic activity and antioxidant mechanisms triggered by FB1 stress. FB1 exposure resulted in significant ethylene emission in a concentration-dependent manner in both genotypes. Moreover, FB1 significantly affected the photosynthetic parameters of PSII and PSI and activated photoprotective mechanisms, such as non-photochemical quenching in both genotypes, especially under 10 µM FB1 concentration. Further, the net photosynthetic rate and stomatal conductance were significantly reduced in both genotypes in a FB1 dose-dependent manner. Interestingly, lipid peroxidation and loss of cell viability were also more pronounced in WT as compared to Nr leaves indicating the role of ethylene in cell death induction in the leaves. Thus, FB1-induced oxidative stress affected the working efficiency of PSI and PSII in both tomato genotypes. However, ethylene-dependent antioxidant enzymatic defense mechanisms were activated by FB1 and showed significantly elevated levels of superoxide dismutase (18.6%), ascorbate peroxidase (129.1%), and glutathione S-transferase activities (66.62%) in Nr mutants as compared to WT tomato plants confirming the role of ethylene in the regulation of cell death and defense mechanisms under the mycotoxin exposure.

Evaluation of Plant Stress Monitoring Capabilities Using a Portable Spectrometer and Blue-Red Grow Light.

Remote sensing offers a non-destructive method to detect plant physiological response to the environment by measuring chlorophyll fluorescence (CF). Most methods to estimate CF require relatively complex retrieval, spectral fitting, or modelling methods. An investigation was undertaken to evaluate measurements of CF using a relatively straightforward technique to detect and monitor plant stress with a spectroradiometer and blue-red light emitting diode (LED). CF spectral response of tomato plants treated with a photosystem inhibitor were assessed and compared to traditional reflectance-based indices: normalized difference vegetation index (NDVI) and photochemical reflectance index (PRI). The blue-red LEDs provided input irradiance and a “window” in the CF emission range of plants (~650 to 850 nm) sufficient to capture distinctive “two-peak” spectra and to distinguish plant health from day to day of the experiment, while within day differences were noisy. CF-based metrics calculated from CF spectra clearly captured signs of vegetation stress earlier than reflectance-based indices and by visual inspection. This CF monitoring technique is a flexible and scalable option for collecting plant function data, especially for indicating early signs of stress. The technique can be applied to a single plant or larger canopies using LED in dark conditions by an individual, or a manned or unmanned vehicle for agricultural or military purposes.

Effects of stem training on the physiology, growth, and yield responses of indeterminate tomato (Solanum lycopersicum) plants grown in protected cultivation

The lower yield of tomatoes grown in tunnels, due to the limited space, remains a challenge. Stem training has long been identified as one of the most important horticultural practices used to improve the yield and fruit quality of tomatoes grown in commercial tunnels; however, there is little information available on the dome-shaped tunnels that are used, particularly by smallholder farmers. The common stem-training methods used in tunnels include the Single-Stem (SS), the Double-Stem (DS) and the Two-Plants-per-Pot (TPP) methods. Their effect on the plants’ growth, development and physiology varies significantly, and hence, it affects crop productivity. The experiment was conducted in an 8 m × 30 m dome-shaped tunnel and the treatment included the single-stem, double-stem and two-plants-per-pot methods. A higher photosynthetic rate was observed in the SS treatment, followed by the DS treatment. Similar trends were found in the growth, yield and fruit quality parameters of the SS and DS treatments. However, the DS and TPP treatments exhibited, on average, a higher number of fruits, as well as a higher colour index, TSS, TA and Brima per harvest, than the SS treatment. The study indicated that the double-stem and two-plants-per-pot training methods are the best for farmers who seek to optimize their yields and maximize their profits for this cultivar.

Effect of vermicompost soil additive on growth performance, physiological and biochemical responses of tomato plants (Solanum lycopersicum L. var. Firenze) to salt stress

Vermicomposting is increasing used to process food, sewage and other organic wastes through the breakdown by earthworms. Vermicompost addition to soils can improve plant growth through increasing the accessability of nutrients and lowering levels of contaminants found in other compost products. This study examins the effect of vermicompost on salinity tolerance in tomato plants (Solanum lycopersicumL., var. Firenze) via greenhouse pot experiments. Plants were grown on 4 substrates designated by letter identifiers: A control, “T” with 100% organic soil; a vermicompost treatment “Vc” was 80% organic soil + 20% Vermicompost; a compost treatment “C” was 80% organic soil + 20% Compost; and a mixture treatment “M” was 80% organic soil + 10% Vermicompost+ 10% Compost. The four treatment groups were exposed to 3 NaCl concentrations (0, 50 and 150 mM); the experimenta; design within the greenhouse was complete randomized block. The plants’ response to salinity stress was evaluated through morphological (shoot length, stem diameter, leaves number, root length, shoot and root fresh and dry weight), physiological (Chla, Chlb and Carotenoid) and biochemical (malondialdehyde (MDA) and catalase (CAT)) parameters. All measured parameters were significantly different between the four soil treatments. Plants grown on Vc substrate showed an improved growth and a better resistance to salinity stress. Analyzed parameters were positively influenced by the contribution of the organic matter (Vermicompost, compost and a mixture of the two) which plays a role in the slow, consistent release of mineral elements and provides soluble nutrients to reduce abiotic stresses. In conclusion, vermicompost could be a relevant method for reducing salt stress on tomato plants growth, addressing the challenges of growing food crops in drier, more saline contaminated envvironments.

Effects of superhydrophobic sand mulching on evapotranspiration and phenotypic responses in tomato (Solanum lycopersicum) plants under normal and reduced irrigation.

Irrigated agriculture in arid and semi-arid regions is a vital contributor to the global food supply. However, these regions endure massive evaporative losses that are compensated by exploiting limited freshwater resources. To increase water-use efficiency in these giga-scale operations, plastic mulches are utilized; however, their non-biodegradability and eventual land-filling renders them unsustainable. In response, we have developed superhydrophobic sand (SHS) mulching technology that is comprised of sand grains or sandy soils with a nanoscale coating of paraffin wax. Here, we investigate the effects of 1cm-thick SHS mulching on the evapotranspiration and phenotypic responses of tomato (Solanum lycopersicum) plants as a model system under normal and reduced irrigation inside controlled growth chambers. Experimental results reveal that under either irrigation scenario, SHS mulching suppresses evaporation and enhances transpiration by 78% and 17%, respectively relative to the unmulched soil. Comprehensive phenotyping revealed that SHS mulching enhanced root xylem vessel diameter, stomatal aperture, stomatal conductance, and chlorophyll content index by 21%, 25%, 28%, and 23%, respectively, in comparison with the unmulched soil. Consequently, total fruit yields, total dry mass, and harvest index increased in SHS-mulched plants by 33%, 20%, and 16%, respectively compared with the unmulched soil. We also provide mechanistic insights into the effects of SHS mulching on plant physiological processes. These results underscore the potential of SHS for realizing food-water security and greening initiatives in arid regions.

The role of photosynthetic activity in the regulation of flg22-induced local and systemic defence reaction in tomato

Flagellin (flg22) induces rapid and long-lasting defence responses. It may also affect the photosynthetic activity depending on several internal and external factors, such as the phytohormone ethylene or the day/night time. Based on the results, flg22 treatment, neither in the light phase nor in the evening, caused any significant change in chlorophyll fluorescence induction parameters in the leaves of wild-type and ethylene-receptor mutant Never ripe tomato plants measured the next morning. However, flg22 in the light phase decreased the effective quantum yield and the photochemical quenching both locally and systemically in guard cells. In parallel, the production of reactive oxygen species and nitric oxide increased, which contributed to the stomatal closure and a decrease in CO2 assimilation the next day. A decrease in sugar content and elevated hexokinase activity measured after flg22 exposure can also contribute to local defence responses in intact tomato plants.

Role of calcium and magnesium on dramatic physiological and anatomical responses in tomato plants

Minerals are the fundamental source of nutrients for plant functions such as photosynthesis, ATP currency, cellular respiration, metabolic activities, defense mechanisms, and tolerance to biotic and abiotic stressors. Minerals are the most significant component of plant nutrition and applying these minerals supplements can increase fruit output. The study’s main aim was to make agricultural farming easier by foliar applying newly created nutrients like Lebosol-calcium and Magnesium. The four treatments: To (Control), T1 (Lebosol-Mg-Plus, 3 ml/L), T2 (Lebosol-Ca-Forte, 3 ml/L), and T3 (Lebosol-Mg-Plus and Lebosol-Ca-Forte, 3 ml/L) was applied as foliar spray to the seedlings of tomato. It was found that T3 substantially enhanced tomato’s morphological features and yield. The treatment T3 significantly increased total soluble protein, chlorophyll content, and antioxidant enzyme activity. Furthermore, the foliar application of T3 considerably improved phenolic and ascorbic acid contents. The general anatomical features of the leaf, stem, and roots of tomato were qualitatively affected by the treatments. Application of Lebosol-Ca provided the highest total thickness of lamina, number of vessel elements, total phloem area, chlorenchyma layer, total area of vessel elements, xylem ratio, and increased palisade layer thickness, vessel diameter. Furthermore, T3 treatment showed a diverse impact on the internal structure of tomato organs, with palisade and spongy parenchyma growing to maximum values and vessel diameters expanding. T3 had also posed remarkable alterations in morpho-physiological, biochemical, and anatomical aspects in tested plants.

Impact of Combined Heat and Salt Stresses on Tomato Plants-Insights into Nutrient Uptake and Redox Homeostasis.

Currently, salinity and heat are two critical threats to crop production and food security which are being aggravated by the global climatic instability. In this scenario, it is imperative to understand plant responses to simultaneous exposure to different stressors and the cross-talk between underlying functional mechanisms. Thus, in this study, the physiological and biochemical responses of tomato plants (Solanum lycopersicum L.) to the combination of salinity (100 mM NaCl) and heat (42 °C; 4 h/day) stress were evaluated. After 21 days of co-exposure, the accumulation of Na+ in plant tissues was superior when salt-treated plants were also exposed to high temperatures compared to the individual saline treatment, leading to the depletion of other nutrients and a harsher negative effect on plant growth. Despite that, neither oxidative damage nor a major accumulation of reactive oxygen species took place under stress conditions, mostly due to the accumulation of antioxidant (AOX) metabolites alongside the activation of several AOX enzymes. Nonetheless, the plausible allocation of resources towards the defense pathways related to oxidative and osmotic stress, along with severe Na toxicity, heavily compromised the ability of plants to grow properly when the combination of salinity and heat was imposed.

Tomato plant responses induced by sparingly available inorganic and organic phosphorus forms are modulated by strigolactones

Background and aimsPhosphorus (P) is an essential nutrient for plant growth, but is also one of the least accessible in soil. Plants have evolved several strategies to cope with P deficiency and recently the role of the phytohormones strigolactones (SLs) in modulating tomato plants acclimation to P shortage has been described. How SLs regulate the use of P from sparingly accessible P sources, such as organic P or precipitated metal-P systems, is however still unknown in tomato.MethodsIn this study, we compared P acquisition strategies of wild-type (WT) and SL-depleted tomato plants grown hydroponically in the presence of dissolved inorganic phosphate (Pi), dissolved myo-inositol hexaphosphate (myoInsP6), or their coprecipitated form following Fe(II) oxidative precipitation.ResultsIrrespective of the P treatment, SL-depleted plants accumulated more P in their tissues than the WT, possibly due to the constitutively higher expression of high-affinity P transporters and activity of P-hydrolyzing enzymes. Wild-type plants were conversely more effective at acidifying their growth medium and exuding more organic compounds in the presence of dissolved myoInsP6 or coprecipitated forms of P, but this behaviour did not translate into a higher P acquisition.ConclusionsThe two genotypes activated different subsets of responses to bypass low P bioavailability, although the P acquisition efficiency (PAE) was not effectively increased. Strigolactone-depleted plants achieved higher PAE values than WT plants regardless of the applied P form, highlighting a central role of SLs in controlling P uptake and optimizing the cost/benefit ratio of P acquisition.

Overexpression of Lectin Receptor-Like Kinase 1 in Tomato Confers Resistance to Fusarium oxysporum f. sp. Radicis-Lycopersici.

The disease Fusarium crown and root rot (FCRR), caused mainly by Fusarium oxysporum f. sp. radicis-lycopersici (FORL), seriously affects commercial tomato [Solanum lycopersicum (Sl)] yields. However, the genes that offer resistance to FORL are limited and the mechanism of resistance to FCRR is poorly understood. Lectin receptor-like kinases (LecRKs) play critical roles in defensive responses and immunity in many plant species; however, whether specific LecRKs are involved in the response of tomato plants to FORL is unclear. Here, we report that the expression of SlLecRK1/Solyc09g011070.1 was obviously induced by the infection of FORL. Biochemical and cell biological data revealed that SlLecRK1 is an active kinase that is located at the cell membrane, while real-time quantitative PCR data suggested that SlLecRK1 is mainly expressed in stems and roots. Genetic studies showed that overexpression of SlLecRK1 significantly improved the resistance of tomato plants to FORL but did not cause visible changes in plant growth and development compared with wild-type control plants. RNA-Seq data suggested that the positive effects of SlLecRK1 on the resistance of tomato plants to FORL occur mainly by triggering the expression of ethylene-responsive transcription factor (ERF) genes. Together, our findings not only identify a new target for the development of FCRR-resistant tomato varieties, they also demonstrate a molecular mechanism linking SlLecRK1 and ERFs in regulating the immune responses of tomato plants to FORL.

Tomato SlPti5 plays a regulative role in the plant immune response against Botrytis cinerea through modulation of ROS system and hormone pathways

While SlPti5 has been shown to play a crucial role in the regulation of antagonistic genes in Solanum lycopersicum and Arabidopsis against pathogen infection, there have been no comprehensive studies on the effects of SlPti5 on the regulatory response mechanism of reactive oxygen species (ROS) system and hormone pathways during growth and disease resistance of tomato plants. Here, we investigated the function of SlPti5 in the defense response of tomato against Botrytis cinerea utilizing a virus-induced gene silencing (VIGS)-based system. Expression profile analysis showed that SlPti5 was significantly induced upon B. cinerea infection, with high expression levels in the leaves and fruit of tomato. VIGS-based silencing of SlPti5 inhibited early vegetative growth, increased the plant's susceptibility to infection, promoted the development of ROS, affected the expression of genes involved in the ROS scavenging system, and attenuated the expression of genes associated with pathogenesis and the ethylene/jasmonic acid signaling pathways. In sum, our data demonstrated that SlPti5 stimulates the immune response of tomato plant to Botrytis cinerea infection by involving the ethylene (ET)- and jasmonic acid (JA)-mediated pathways and modulating the expression of some key pathogenesis-related (PR) genes.

Jasmonate and Melatonin Act Synergistically to Potentiate Cold Tolerance in Tomato Plants

Both jasmonic acid (JA) and melatonin (MT) have been demonstrated to play positive roles in cold tolerance, however, whether and how they crosstalk in the cold responses in plants remain elusive. Here, we report that JA and MT act synergistically in the cold tolerance in tomato plants (Solanum lycopersicum). It was found that JA and MT were both substantially accumulated in response to cold stress and foliar applications of methyl jasmonate (MeJA) and MT promoted cold tolerance as evidenced by increased Fv/Fm, decreased relative electrolyte leakage (EL) and declined H2O2 accumulation in tomato plants. Inhibition of MT biosynthesis attenuated MeJA-induced cold tolerance, while inhibition of JA biosynthesis reduced MT accumulation in tomato plants under cold conditions. Furthermore, qRT-PCR analysis showed that the expressions of two MT biosynthetic genes, SlSNAT and SlAMST, were strongly induced by MeJA, whereas suppression of SlMYC2, a master JA signaling regulator, abated the expressions of SlSNAT and SlAMST under cold stress. Additionally, suppression of SlMYC2 reduced MT accumulation, decreased Fv/Fm and increased EL in cold-stressed tomato plants. Interestingly, exogenous MT promoted JA accumulation, while inhibition of MT biosynthesis significantly reduced JA accumulation in tomato plants under the cold condition. Taken together, these results suggest that JA and MT act cooperatively in cold tolerance and form a positive feedback loop, amplifying the cold responses of tomato plants. Our findings might be translated into the development of cold-resistant tomato cultivars by genetically manipulating JA and MT pathways.

Exogenous hexanoic acid induced primary defense responses in tomato (Solanum lycopersicum L.) plants infected with Alternaria solani

The effect of exogenous hexanoic acid on Alternaria solani-infected tomato plants was investigated in the present study. Tomato seedlings treated with different doses of hexanoic acid were inoculated with A. solani and their antioxidative, biochemical and molecular responses were studied. Results indicated that hexanoic acid application increased hydrogen peroxide content by 49% and peroxidase activity by 408% in the infected plants, while ascorbate peroxidase and catalase activities and total polyphenol content were not significantly changed. It was revealed that the expression of NAM, ATAF1and 2, and CUC2 family multiple stress-responsive transcription factor (SlNAC1), Mitogen-activated protein kinase 3 (SlMAPK3), increased 13 and 4 folds, respectively, while the expression increase for pathogenesis related proteins type1) (SlPR1) was 57% in A. solani-infected plants. It was also revealed that SlPR1 expression in plants treated with 1 mM hexanoic acid reached its highest level 72 h post inoculation while highest superoxide dismutase (SlSOD) expression rate was recorded 24 h after inoculation. It seems that exogenous application of hexanoic acid increased reactive oxygen species (ROS) content in A. solani-infected plants and subsequently induced systemic acquired resistance (SAR) pathways which in turn enhanced expression of superoxide dismutase (SOD), NAC1 and MAPK3 genes. These findings indicated that hexanoic acid induced resistance in tomato plants against A. solani by enhanced SAR signaling network leading to enhanced defense responses. While Ja/Et pathway is believed to be the prevalent biochemical pathway involved in plant resistance to necrotrophic pathogens, our findings revealed that hexanoic acid-treated tomato plants activated defense responses though SAR which is mainly a salicylic acid-dependent signaling pathway.

Expression analysis of Some Stress-Related Genes Induced by Cadmium on Tomato (Solanum lycopersicum L.) Plants

Environmental pollution occurs in nature as air, soil and water pollution and as a result it affects whole ecosystem including human beings. Although industrialization and technological developments have made life easier than before, in recent years, they have triggered environmental pollution. Cadmium, which is a toxic pollutant for all living things, is one of the most important element in heavy metal pollutants. In this study, it was aimed to determine gene expression changes in tomato plant under Cd stress. Molecular response of tomato plants to Cd stress was examined by transcript accumulation analysis of two stress-related genes: (i) MT-2 (metallothionine-2) gene encodes metal binding protein and (ii) The GR-1 (glutathione reductase-1) gene encodes the glutathione reductase enzyme and is a marker of the ROS scavenging mechanism. Expression differences in MT-2 and GR-1 genes in tomato seedlings exposed to cadmium stress at different concentrations ranging from 20 to 1280 mg L-1 for 24 hours were determined performing quantitative real-time PCR. The results obtained from this study were showed that MT-2 and GR-1 genes play an important role in the mechanism of protection against heavy metal of Cd stress. In addition, the physiological properties of tomato have been associated with cadmium accumulation.

Strobilurins and carboxamides in physiology and management of Alternaria sp. of tomato

The aim of this study was to evaluate the application effects of fungicides based on strobilurins and carboxamides on the physiology, management of Alternaria sp. and production of tomato fruits in a protected environment. Two experiments were carried out in Salto-SP in 2018 and 2019. The randomized block design was used, with seven treatments: inoculated control (application of water and inoculum with Altenaria sp.), absolute control (application of water), azoxystrobin, boscalid, boscalid + azoxystrobin, pyraclostrobin and boscalid + pyraclostrobin, and five replications. To verify the photosynthetic efficiency of plants, analysis of chlorophyll a fluorescence and leaf gas exchange was performed at 50, 95 and 120 DAT. It was also verified the disease severity (%) and finally and the total and commercial production of fruits. From the results, under this research conditions, the tested fungicide application promoted positive physiological responses in tomato plants, controlling early blight disease and increasing fruit production. The application of the fungicides boscalid and azoxystrobin + boscalid can be a management tool for Alternaria sp. for tomatoes, for controlling the progress of the disease as well as for promoting better physiological and productive responses.

INFLUNCE OF CULTIVARS, PLANT GEOMETRY, HUMIC ACID AND THEIR INTERACTIONS ON GROWTH AND YIELD OF NIGER (Guizotia abyssinicia Cass.) CROP

INFLUNCE OF CULTIVARS, PLANT GEOMETRY, HUMIC ACID AND THEIR INTERACTIONS ON GROWTH AND YIELD OF NIGER (Guizotia abyssinicia Cass.) CROP

Commonalities of Molecular Response in Tomato Plants against Parasitic Nematodes

Commonalities of Molecular Response in Tomato Plants against Parasitic Nematodes

Abscisic acid is required for root elongation associated with Ca2+ influx in response to water stress

Abscisic acid (ABA) is a critical hormone for plant survival upon water stress. In this study, a large-scale mutants of Arabidopsis ecotype Columbia-0 (Col-0) by ethyl methanesulfonate (EMS)-mutagenesis were generated, and an improved root elongation under water-stress 1 (irew1) mutant showing significantly enhanced root growth was isolated upon a water potential gradient assay. Then, irew1 and ABA-related mutants in Arabidopsis or tomato plants were observed under water potential gradient assay or water-deficient condition. ABA pathway, Ca2+ response and primary root (PR) elongation rate were monitored in addition to DNA- and RNA-Seq analyses. We found that based on phenotyping and transcriptional analyses, irew1 exhibited the enhanced PR growth, ABA and Ca2+ responses compared to wild-type subjected to water stress. Interestingly, exogenous Ca2+ application enhanced PR growth of irew1, ABA-biosynthesis deficient mutants in Arabidopsis and tomato plants in response to water potential gradients or water-deficient condition. In combination with other ABA-related mutants and pharmacological study, our results suggest that ABA is required for root elongation associated with Ca2+ influx in response to water stress.

Conditioned soils reveal plant-selected microbial communities that impact plant drought response

Rhizobacterial communities can contribute to plant trait expression and performance, including plant tolerance against abiotic stresses such as drought. The conditioning of microbial communities related to disease resistance over generations has been shown to develop suppressive soils which aid in plant defense responses. Here, we applied this concept for the development of drought resistant soils. We hypothesized that soils conditioned under severe drought stress and tomato cultivation over two generations, will allow for plant selection of rhizobacterial communities that provide plants with improved drought resistant traits. Surprisingly, the plants treated with a drought-conditioned microbial inoculant showed significantly decreased plant biomass in two generations of growth. Microbial community composition was significantly different between the inoculated and control soils within each generation (i.e., microbial history effect) and for the inoculated soils between generations (i.e., conditioning effect). These findings indicate a substantial effect of conditioning soils on the abiotic stress response and microbial recruitment of tomato plants undergoing drought stress.

A Transcriptome Analysis Revealing the New Insight of Green Light on Tomato Plant Growth and Drought Stress Tolerance.

Light plays a pivotal role in plant growth, development, and stress responses. Green light has been reported to enhance plant drought tolerance via stomatal regulation. However, the mechanisms of green light-induced drought tolerance in plants remain elusive. To uncover those mechanisms, we investigated the molecular responses of tomato plants under monochromatic red, blue, and green light spectrum with drought and well-water conditions using a comparative transcriptomic approach. The results showed that compared with monochromatic red and blue light treated plants, green light alleviated the drought-induced inhibition of plant growth and photosynthetic capacity, and induced lower stomatal aperture and higher ABA accumulation in tomato leaves after 9 days of drought stress. A total of 3,850 differentially expressed genes (DEGs) was identified in tomato leaves through pairwise comparisons. Functional annotations revealed that those DEGs responses to green light under drought stress were enriched in plant hormone signal transduction, phototransduction, and calcium signaling pathway. The DEGs involved in ABA synthesis and ABA signal transduction both participated in the green light-induced drought tolerance of tomato plants. Compared with ABA signal transduction, more DEGs related to ABA synthesis were detected under different light spectral treatments. The bZIP transcription factor- HY5 was found to play a vital role in green light-induced drought responses. Furthermore, other transcription factors, including WRKY46 and WRKY81 might participate in the regulation of stomatal aperture and ABA accumulation under green light. Taken together, the results of this study might expand our understanding of green light-modulated tomato drought tolerance via regulating ABA accumulation and stomatal aperture.