Revealing the response laws of soils under various mining conditions is of significance for the scientific protection of farmland in mining areas. To avoid the interference from the spatial heterogeneity of natural soil on the research results, similar simulation experiments were employed. Two models for soil responses under mining with and without grouting into overburden bed-separation were designed, and monitoring was conducted for soil quality factors (moisture content, silt and clay content, and bulk density) and surface deformation factors (subsidence, inclined, and horizontal deformation). The results indicate that it can significantly control surface subsidence in mining with grouting into overburden bed-separation, the maximum subsidence value was reduced by 66.67%, the average subsidence value of each measuring point in the main section was reduced by 78.92%, and the subsidence area was reduced by 34.68%. The coefficient of variation of soil quality factors in the mining-affected area was obviously higher than that in the control area and the initial value before mining. Compared with mining without grouting, the variation coefficient of soil quality factor under mining with grouting was reduced by 2.71-6.79%. Soil quality factors and surface deformation factors show a significant quadratic function relationship, among which horizontal deformation has the greatest influence on soil quality factors, followed by subsidence and inclination. Therefore, it can effectively control surface subsidence and protect cultivated land when mining with grouting into overburden bed-separation.