Offshore wind turbines are often supported on monopiles and are always subjected to long-term cyclic loading during their service life. This cyclic loading induces changes in the damping, stiffness and permanent accumulated rotation of the monopile foundation. The main purpose of this paper is to investigate the effect of these three changes occurring simultaneously on the dynamic response and fatigue life of offshore wind turbines in sand. To this end, an integrated methodology is presented based on time-domain finite element model and small-scale model tests of rigid piles. Three states of the monopile foundation are selected and defined based on the operational time of the turbine. The results show that these three changes have a slight effect on the dynamic response of offshore wind turbines, but have a significant effect on the fatigue life. The fatigue life decreased from 23.3 years for the initial state to 20.99 years for the medium state and 19.45 years for the ultimate state, a decrease of 10% and 16.5%, respectively, indicating that these changes should be addressed in the design of the fatigue life calculation of offshore wind turbine structures. The systematic parametric analysis shows that soil damping has the greatest effect on the dynamic response and fatigue life, followed by soil stiffness, which is less affected by permanent accumulated rotation.