Utilization Of Waste Resources Research Articles

Optimizing tomato waste hydrolysate for enhanced fucoxanthin biosynthesis in mixotrophic cultivation of Isochrysis galbana

Vegetable waste, rich in bioactive compounds, offers a promising resource for producing value-added products. This study explored the use of tomato waste, containing glucose (40 mg/g), lycopene (95.12 μg/g), and β-carotene (24.31 μg/g), for cultivating fucoxanthin-rich Isochrysis galbana. Water-soluble lycopene (2.0 μg/mL) and β-carotene (0.4 μg/mL) effectively upregulated key carotenoid synthesis genes and boosted cell growth and fucoxanthin production (3.64 and 3.60 pg/cell, respectively) within 10 days in a mixotrophic culture. Optimized tomato waste hydrolysate achieved a high cell density of 1.21 × 107 cells/mL, 2.13 g/L biomass, and 21.02 mg/g fucoxanthin. This study highlights the potential of combining tomato waste with microalgae for a novel and innovative approach towards waste management and resource utilization.

Stepwise dechlorination and co-pyrolysis of poplar wood with dechlorinated polyvinyl chloride: Synergistic effect and products distribution

With the combination of stepwise dechlorination and co-pyrolysis techniques, this study conducted polyvinyl chloride (PVC) dechlorination experiments and co-pyrolysis experiments of poplar wood (PW) with dechlorinated polyvinyl chloride (DPVC) by thermogravimetric analysis and a fixed bed reactor. Stepwise pyrolysis effectively removed Cl from PVC with a dechlorination efficiency of 99.84 % at 360 °C for 30 min. Thermogravimetric tests and thermokinetic variables were employed to describe the co-pyrolysis process's thermodynamic behavior, where co-pyrolysis significantly diminished the activation energy of the initial pyrolysis stage (9.65–21.62 kJ/mol) and increased the reaction rate (0.02–0.09 %/°C). The synergistic effect of co-pyrolysis enhanced the yield and quality of liquid oil and reduced the solid residue rate, with the maximum change in solid residue rate (−2.36 wt%) occurring at PW:DPVC = 3:7. The optimal conditions for the synergistic effect are a raw material ratio of 3:7 at 500 °C. Co-pyrolysis efficiently reduced the content of oxygen-containing compounds of phenols, ketones, and acids in oil, and elevated the selectivity of aromatics. The research methods avoid the drawbacks of bio-oil and plastic oil and improve the quality of pyrolysis oil in a concise and efficient manner, which provides some new ideas for the resource and clean utilization of municipal waste.

Interstitial Boron Atoms in Pd Aerogel Selectively Switch the Pathway for Glycolic Acid Synthesis from Waste Plastics.

Electro-reforming of poly(ethylene terephthalate) (PET) into valuable chemicals is garnering significant attention as it opens a mild avenue for waste resource utilization. However, achieving high activity and selectivity for valuable C2 products during ethylene glycol (EG) oxidation in PET hydrolysate on Pd electrocatalysts remains challenging. The strong interaction between Pd and carbonyl (*CO) intermediates leads to undesirable over-oxidation and poisoning of Pd sites, which hinders the highly efficient C2 products production. Herein, a nonmetallic alloying strategy is employed to fabricate a Pd-boron alloy aerogel (PdB), wherein B atoms are induced to regulate the electron structure and surface oxophilicity. This approach allows a remarkable mass activity of 6.71 A mgPd -1, glycolic acid (GA) Faradaic efficiency (FE) of 93.8%, and stable 100 h cyclic electrolysis. In situ experiments and density functional theory calculations reveal the contributions of B inserted in Pd lattice on highly effective EG-to-GA conversion. Interestingly, the heightened surface oxophilicity and regulated electronic structure by B incorporation weakened *CO intermediates adsorption and enhanced hydroxyl species affinity to accelerate oxidative *OH adspecies formation, thereby synergistically avoiding over-oxidation and boosting GA synthesis. This work provides valuable insights for the rational design of high-performance electrocatalysts for GA synthesis via an oxophilic B motifs incorporation strategy.

Enhancement of alkaline pretreatment-anaerobically digested sludge dewaterability by chitosan and rice husk powder for land use of biogas slurry

Alkaline pretreatment can improve the methane yields and dewatering performance of anaerobically digested sludge, but it still needs to be coupled with other conditioning methods in the practical dewatering process. This study utilized four different flocculants and a skeleton builder for conditioning of alkaline pretreatment-anaerobically digested sludge. Chitosan was found to be the most effective in dewatering the sludge. Chitosan coupled with rice husk powder further improved the dewatering performance, which reduced normalized capillary suction time, specific resistance to filtration, and moisture content by 98.7%, 82.0%, and 12.1%. For land use of biogas slurry as a fertilizer, chitosan conditioning promoted the growth of corn seedlings, while the other three flocculants diminished the growth of corn seedlings. Chitosan coupled with rice husk powder further promoted the growth of corn seedlings by 103.5%, 65.0%, and 53.7% in fresh weight, dry weight, and root length, respectively. Overall, chitosan coupled with rice husk powder not only enhanced the dewaterability of alkaline pretreatment-anaerobically digested sludge but also realized the resource utilization of agricultural waste.

Reducing moisture content can promote the removal of pathogenic bacteria and viruses from sheep manure compost on the Qinghai-Tibet Plateau

Livestock manure contains a high concentration of pathogenic bacteria, viruses, and other harmful microorganisms. The effective elimination of these pathogens is of great significance to the harmless and resource utilization of livestock and poultry manure. Building upon prior research, this study further explored the removal efficiency of pathogenic bacteria and viruses in sheep manure compost on Qinghai-Tibet Plateau under 45 % (M45) and 60 % (M60) moisture content. The results showed that M45 led to a significant increase in the average composting temperature (P＜0.05), a notable reduction in the levels of Salmonella and Coliforms in the compost products, and facilitated the decline in abundance of various pathogens. As composting progressed, the abundance of viruses declined rapidly. Uroviricota was the most dominant viral phylum, and Siphoviridae, Myoviridae, Herpesviridae, and Podoviridae as the predominant viral families. M45 can significantly reduce the diversity and abundance of compost virus community (P＜0.05). The correlation analysis indicated a negative relationship between pathogens and the average temperature and humus content of the compost. In summary, the reduction of moisture content can enhance the biosafety of sheep manure compost products on the Qinghai-Tibet Plateau. These findings provide theoretical basis and technical support for the harmless and resource utilization of sheep manure waste on the Qinghai-Tibet Plateau.

Electrocatalytic formate synthesis from polyethylene terephthalate and carbon dioxide through sulfide-and-reconstruct engineering of catalyst

The global issue of plastic pollution and carbon emission have significantly impacted the survival of both ecosystems and human beings. Electrochemical coupling of polyethylene terephthalate plastic (PET) and CO2 into fine chemicals provides a potential approach to achieve waste resource utilization and carbon neutrality. Herein, we reported an innovative electrocatalytic system for concurrently upgrading PET hydrolysate and CO2 into value-added formic acid. Through a sulfide-and-reconstruct strategy, Ni3Bi2S2 and Bi2S3 were synthesized as pre-catalysts, which can undergo in situ reconstruction to form NiOOH/Ni3Bi2S2 at the anode and Bi (1.5 S) at the cathode during electrolysis. The exceptional electrocatalytic performance (96 % and 97 % at the anode and cathode, respectively) and stabilities (>30 h) of the anodic NiOOH/Ni3Bi2S2 and cathodic Bi (1.5 S) was attributed to the exposure of the true active site after reconstruction. By coupling PET hydrolysate oxidation reaction (POR) with CO2 reduction reaction (CO2RR), the assembled zero-gap MEA electrolyzer realized a total Faradaic efficiency of 175 % for formate. This study provided a concept for upcycling of PET.

Evolutionary game and simulation analysis of construction waste recycling from the perspective of stakeholders.

In current construction waste resource management processes, the effect of government supervision is unclear, and illegal treatment and low-quality reproduction of recycling and reprocessing enterprises by construction units are common. To improve the degree of resource utilization of construction waste and deeply explore the role of its key influencing factors, a tripartite evolutionary game model of construction waste resource treatment in which the government, construction units, and recycling and reprocessing enterprises are the research objects that considers public participation factors to be established. MATLAB is used to simulate the sensitivity of relevant parameters. The results show that: (1) An increase in government fines can regulate the behavior of enterprises; (2) Low government subsidies are conducive to the development of a tripartite stability strategy; and (3) An increase in the cost difference between the two strategies of the enterprise will weaken its willingness to carry out green operations (and after the cost difference exceeds the threshold, the enterprise will refuse to carry out green operations); (4) The reputation value brought by the public and the additional value added by reputation under the contrast effect have an incentive effect on the enterprise and the government; and (5) The peak value of the inverted U-shaped curve of government strategy choice is affected by the degree of public participation. Therefore, the government should propose rectifications in terms of fines and subsidies, and both companies can use technological innovation to reduce costs. At the same time, it is necessary to raise stakeholders' awareness of resource utilization and encourage the public to actively participate in supervision. The research conclusions can provide a decision-making reference for improving the utilization of construction waste resources and the efficient treatment of construction waste resources.

Synergistic effect of Cerium and Niobium enhances the low-temperature NH3-SCR activity of Fe-containing solid waste

To develop environment-friendly low temperature NH3-SCR catalyst and realize the resource utilization of industrial solid waste, this research prepared a series NH3-SCR catalysts by using Fe-containing solid waste loading CeO2 and Nb2O5. It was found that the Ce2Nb1/Fe-Si catalyst exhibited the highest NH3-SCR activity, reaching about 93 % NOx conversion and 99 % N2 selectivity at 300 °C due to the synergistic effect between Ce and Nb species. The characterization results showed that the interaction between Ce and Nb was stronger than that between Nb and Fe species, promoting the NH3 adsorption and redox cycle of Ce4+ + Nb4+ ↔ Ce3+ + Nb5+, Ce3+ + Fe3+ ↔ Fe2+ + Ce4+, which increased the contents of Fe2+ and surface adsorbed oxygen. Moreover, the co-existence of Ce and Nb species would increase the NOx adsorption ability and promote the oxidation of NO to NO2, which was beneficial for “Fast SCR” reaction. Finally, the introduction of CeO2 and Nb2O5 did not changed the reaction mechanism, followed by a Langmuir-Hinshelwood mechanism.

Performance optimization design of high ductility concrete rapid repair material incorporating coal gangue aggregate

In order to solve the problem of efficient and effective repair after brittle cracking of concrete on road and bridge deck pavements, and to consider the resource utilization of coal gangue solid waste, the river sand was replaced by coal gangue and a high ductile concrete rapid repair material (HDCRRM) was prepared through performance optimization design. First, the fluidity and compressive strength of materials mixed with different amounts of sulfoaluminate cement (SAC), fly ash, lithium carbonate, calcium hydroxide, calcium formate, sand-binder ratio, water-binder ratio and polycarboxylate superplasticizer were studied, so as to determine the preferred benchmark mix proportion. Then, the replacement rate of coal gangue was optimized to develop HDCRRM. Test results show that the reduction of SAC cement and the increase of fly ash content can weaken the compressive strength of material. The rational design content of lithium carbonate, calcium hydroxide, calcium formate, sand-binder ratio, water-binder ratio and polycarboxylate superplasticizer all can improve the fluidity and compressive strength of the materials within a certain range. When the replacement rate of coal gangue aggregate increases, the tensile elastic modulus and ultimate tensile strength of HDCRRM material decrease, while the ultimate tensile strain increases. When the replacement rate of coal gangue aggregate is 100 %, the compressive strength of HDCRRM is 40.5 MPa, the ultimate tensile strain is 2.83 %, and the average crack width is 50.27μm. The early compressive strength of HDCRRM is mainly provided by a large amount of ettringite and C-S-H gel.

Study on the removal of Cd from dewatered sludge by combined extraction agents: Peel extract and compound chemical extraction agent

The recycling of fruit peel resources is a current research hotspot. This study screened and configured a composite extractant consisting of peel and chemical extractant through extraction experiments and explored the heavy metals(HMs) release effect. The results showed that citrus reticulata(CR), citrullus lanatus (CL), 0.16 mol·L−1 nitrilotriacetic acid(NTA) with 0.04 mol·L−1 oxalic acid(NO5), and 0.12 mol·L−1NTA with 0.08 mol·L−1tartaric acid(NT4) had the strongest extraction ability. After the cross-combination was optimized, CR-NO5 (1:50, 6 h, 35 °C) and CL-NT4 (1:50, 36 h, 45 °C) had the highest extraction rates for Cd and Zn, which were 92.6 % and 98.4 %, respectively. The CL series increased the nutrient content of sludge (157.75–177.88 g·kg−1). The four combined extractants increased the proportion of soluble components of HMs in sludge (14–36 %). Therefore, the combined leaching agent will provide a valuable reference for the harmless treatment of HMs in sludge and the resource utilization of peel waste.

Zn-doped bentonite catalysts for waste engine oil cracking: Thermal treatment offers a cleaner alternative to acid activation

This study investigates the cracking of waste engine oil (WEO) using bentonite clay (BC) activated through acid and thermal methods, as well as with Zn-doped BCs. Acid activation with 1 M HCl and Zn doping did not alter the BC catalyst’s structure. XRD and SAED analyses confirmed the crystallinity, while TEM showed partial destruction of the layered structure. XRF analysis indicated reduced Al and Si content upon Zn-doping due to high cation exchange capacity. Zn-doping decreased surface area and pore volume but increased acidic sites in acid-treated catalysts, although Zn suppressed these sites in calcined BC. Zn-doped catalysts displayed higher thermal stability. Cracking WEO at 450 °C for 45 min, with and without catalysts, yielded high liquid product recovery (72.6 %–82.8 %), primarily carboxylic acids and aromatics. This demonstrates the potential of Zn-doped BC as an effective catalyst for converting WEO into valuable products, aiding waste management and resource utilization.

Activation of peroxymonosulfate by FeOOH modified biochar for the degradation of tetracycline hydrochloride

For preventing environmental pollution and realizing resource utilization of agricultural and forestry wastes, biochar was formed after high temperature pyrolysis of citrus peels under anoxic conditions, and employed as catalyst to activate peroxymonosulfate (PMS) for degrading tetracycline hydrochloride (TC) in wastewater. Furthermore, α-FeOOH modified biochar was fabricated through an alcohol-assisted hydrothemal method for proving the catalytic activity of biochar. The prepared catalysts were characterized by several characterization techniques. Results showed that the catalytic activity of α-FeOOH modified biochar was much better than that of biochar or α-FeOOH, which is attributed to the synergistic effects of biochar and α-FeOOH on the PMS activation. Meanwhile, the effects of PMS concentration, co-existing ions (Cl-, NO3−), and humic acids (HA) on the degradation of TC were investigated through static experiments. More importantly, radical trapping experiment tests suggested that hydroxyl radical (•OH), sulfate radical (SO4•-), and singlet oxygen (1O2) were generated during the process of catalyst activation of PMS, and 1O2 played the main role for TC degradation.

Synthesis of Iron-Tailing-Based porous ceramic granules for the adsorption of phenol-containing wastewater degradation

To achieve the resource utilization of solid waste and the treatment of phenol-containing wastewater in China, a new type of ceramic granule (TBBFSITFA) with the function of degrading phenol wastewater has been prepared from titanium-containing blast furnace slag (TBBFS), iron tailings (IT), and fly ash (FA) as raw materials to achieve the sustainable development of “treating waste with waste.” The adsorption behavior of TBBFSITFA samples on phenol in an aqueous solution was experimentally investigated. Double-beam ultraviolet–visible spectrophotometer (UV–vis), total organic carbon (TOC) test showed that the optimal raw material ratio of TBBFS: IT: FA = 10:70:20, the degradation rate of phenol reached 95.40%, and the removal rate of TOC was as high as 93.29%. In addition, X-ray photoelectron spectroscopy (XPS), energy spectrometry (EDS), and Fourier transform infrared spectrophotometry (FT-IR) demonstrated that the better the synergistic effect produced by having a suitable Fe3+/Fe2+ ratio and raw material ratio in the TBBFSITFA sample, as well as a high specific surface area, porosity and high surface adsorption of oxygen, the higher the mineralization of the phenol solution. This work provides a low-cost, environmentally friendly, and simple technology for phenol-containing wastewater treatment and a new idea for effectively using solid waste resources.

An Empirical Research on the Relationship Between Comprehensive Utilization of Waste Resources and Environmental Governance

An Empirical Research on the Relationship Between Comprehensive Utilization of Waste Resources and Environmental Governance

Adsorption characteristics and mechanisms of bisphenol A on novel nitrogen-modified biochar derived from waste masks and biomass.

Resource utilization of waste masks has become an urgent scientific issue. In this work, with sustainably, waste masks and biomass were co-pyrolysis with oxygen limitation to prepare mask-based biochar (MB). Then, urea was introduced to prepare novel nitrogen modified mask-based biochar (NMB) via a one-step hydrothermal synthesis method. The adsorption characteristics of NMB on the emerging environmental pollutant, bisphenol A (BPA), were evaluated via batch adsorption tests. Moreover, the physicochemical properties of the materials were characterized with various advanced techniques. Also, the roles of waste masks and nitrogen modification were explored. The adsorption mechanisms of NMB on BPA were revealed as well as the performance differences between different adsorbents. The results showed that waste masks participated in thermochemical reactions, shaped the microsphere structure of biochar, and increased the types of surface functional groups. The nitrogen modification enriched the surface elemental composition and activated the specific surface area via the mesopore. These would enhance the adsorption performance. The maximum adsorption of BPA by NMB was 62.63mg·g-1, which was approximately 2.35-5.58 times higher than that of the control materials. Temkin model and pseudo-second-order model optimally simulate the isothermal and kinetic adsorption, respectively. The adsorption mechanisms are jointly by physical and chemical adsorption, which mainly includes π-π interaction, hydrogen bonding, intraparticle diffusion, surface adsorption, and ion exchange. After discussion and evaluation, NMB has lower preparation process cost (7.21 USD·kg-1) and safety, with potential for environmental applications. This study aims to expand new ideas for the comprehensive utilization of waste masks and the preparation of eco-friendly materials. Moreover, it provides a theoretical basis for the removal of BPA.

Selective hydrolysis of traditional Chinese medicine residue into reducing sugars catalysed by sulfonated carbon catalyst and application of hydrolysate

The conversion of waste into high-value products has long been a focus point of research in green and sustainable chemistry. In this study, a novel value-adding strategy was devised to not only facilitate the resource utilization of biomass waste like Chinese medicine residue (CMR) and pomelo peel but also contribute to the advancement of microalgae cultivation. Sulfonated biochar PP-700-S was synthesized from pomelo peel waste, and its morphology and structural characteristics were analyzed. An effective method for the hydrolysis of CMR using PP-700-S as a catalyst in the aqueous phase was then explored, with a thorough investigation of the process and key influencing factors. Under the reaction conditions of 130 °C, 24 h, 20 mL H2O, CMR: PP-700-S = 1: 1, a 51.87 % yield of reducing sugars was directly obtained from the CMR, with PP-700-S exhibiting the selectivity of glucose production. Subsequently, the hydrolysate (HL-CMR) was utilized as a growth medium for microalgae, revealing that the presence of HL-CMR enhanced the nutrient balance in the medium, creating a more conducive environment for microalgae growth. Overall, this study presents a sustainable and efficient pathway for the utilization of lignocellulosic waste, facilitating the conversion of waste into higher-value products and contributing to the development of a green and sustainable science.

Co-pyrolysis of wheat straw with polyester-based polyurethane for nitrogenous compounds: Pyrolysis kinetic properties and synergistic effects

To re-utilize agricultural waste straw and waste plastic, the co-pyrolysis process of wheat straw (WS) and high-nitrogen polymer polyester-based polyurethane (PU) and the synergistic effects were studied. The results showed that PU facilitated pyrolysis to occur at lower temperatures. The effective apparent activation energies (Eα) of different samples were calculated at different conversion rates with three methods (Kissinger, KAS and Starink). It was found that PU decreased the Eα values and enthalpy change of WS and increased the reactivity. Both ΔG and ΔS results indicate that a heating rate of 5 ℃/min is more favorable for co-pyrolysis. PU greatly promoted the production of pyrolysis oil. Co-pyrolysis oil doped with 75 % PU gave the highest yield of 77 wt%. Co-pyrolysis effectively suppressed the production of acids, aldehydes, and phenols in pyrolysis oils. It increased the levels of alcohols and ketones, improving the pyrolysis oil's stability and calorific value. α, β-unsaturated aldehydes produced by WS during pyrolysis reacted with hydrazine derivatives produced by PU pyrolysis to produce pyrazole compounds after dehydration. In the co-pyrolysis of 50 % doped PU, the reaction of methyl and methylene produced by WS with amines (-NH2) produced by the pyrolysis of PU promotes the production of trimethylamine. The yield of amines in the nitrogenous compounds reached a maximum at this time. PU increased the yield of nitrogenous compounds and their variety. It favored the output of high-value chemicals. FT-IR analyses of pyrolytic charcoal showed that the addition of PU promoted the cleavage of WS and the dehydration and decarboxylation reactions of C-O and C-O-C. Finally, synergistic effects and nitrogen conversion mechanisms in the co-pyrolysis process are proposed. This work provided a theoretical basis for the resource utilization of WS waste and PU waste.

Risk of hydrogen sulfide pollution from pressure release resulting from landfill mining

Landfill mining (LFM) has gained widespread recognition due to its benefits in terms of resource utilization of landfill waste and reuse of landfill sites. However, it is important to thoroughly assess the associated environmental risks. This study simulated the pressure release induced from LFM in small-scale batch anaerobic reactors subject to different initial pressures (0.2–0.6 MPa). The potential risk of hydrogen sulfide (H2S) pollution resulting from pressure release caused by LFM was investigated. The results demonstrated that the concentration of H2S significantly increased following the simulated pressure treatments. At the low (25 °C) and high (50 °C) temperatures tested, the peak H2S concentration reached 19366 and 24794 mg·m−3, respectively. Both of these concentrations were observed under highest initial pressure condition (0.6 MPa). However, the duration of H2S release was remarkably longer (>90 days) at the low temperature tested. Microbial diversity analysis results revealed that, at tested low temperature, the sulfate-reducing bacteria (SRB) communities of various pressure-bearing environments became phylogenetically similar following the pressure releases. In contrast, at the high temperature tested, specific SRB genera (Desulfitibacter and Candidatus Desulforudis) showed further enrichment. Moreover, the intensified sulfate reduction activity following pressure release was attributed to the enrichment of specific SRBs, including Desulfovibrio (ASV585 and ASV1417), Desulfofarcimen (ASV343), Candidatus Desulforudis (ASV24), and Desulfohalotomaculum (ASV506 and ASV2530). These results indicate that the pressure release associated with LFM significantly increases the amount of H2S released from landfills, and the SRB communities have different response mechanisms to pressure release at different temperature conditions. This study highlights the importance of considering the potential secondary environmental risks associated with LFM.

Evolutionary Game Analysis for Promoting the Realization of Construction Waste Recycling and Resource Utilization: Based on a Multi-Agent Collaboration Perspective

Evolutionary Game Analysis for Promoting the Realization of Construction Waste Recycling and Resource Utilization: Based on a Multi-Agent Collaboration Perspective

Trilogy of comprehensive treatment of kitchen waste by bacteria-microalgae-fungi combined system: Pretreatment, water purification and resource utilization, and biomass harvesting

Given its profound disservice, a bacteria-microalgae-fungi combined system was designed to treat kitchen waste. Firstly, a new type of microbial agent homemade compound microorganisms (HCM) (composed of Serratia marcescens, Bacillus subtilis and other 11 strains) with relatively high bio-security were developed for pretreating kitchen waste, and HCM efficiently degraded 85.2 % cellulose, 94.3 % starch, and 59.0 % oil. HCM also accomplished brilliantly the initial nutrients purification and liquefaction conversion of kitchen waste. Under mono-culture mode (fungi and microalgae were inoculated separately in the pre - and post-stages) and co-culture mode (fungi and microalgae were inoculated simultaneously in the early stage), microalgae-fungi consortia were then applied for further water purification and resource utilization of kitchen waste liquefied liquid (KWLL) produced in the pretreatment stage. Two kinds of microalgae-fungi consortia (Chlorella sp. HQ and Chlorella sp. MHQ2 form consortia with pellet-forming fungi Aspergillus niger HW8–1, respectively) removed 79.5–83.0 % chemical oxygen demand (COD), 44.0–56.5 % total nitrogen (TN), 90.3–96.4 % total phosphorus (TP), and 64.9–71.0 % NH4+-N of KWLL. What's more, the microalgae-fungi consortia constructed in this study accumulated abundant high-value substances at the same time of efficiently purifying KWLL. Finally, in the biomass harvesting stage, pellet-forming fungi efficiently harvested 81.9–82.1 % of microalgal biomass in a low-cost manner through exopolysaccharides adhesion, surface proteins interaction and charge neutralization. Compared with conventional microalgae-bacteria symbiosis system, the constructed bacteria-microalgae-fungi new-type combined system achieves the triple purpose of efficient purification, resource utilization, and biomass recovery on raw kitchen waste through the trilogy strategy, providing momentous technical references and more treatment systems selection for future kitchen waste treatment.