Traveling athletes experience fatigue from flights and time zone changes, which increases their subjective burden and can affect their sports performance, increase the risk of illness and injury. Literature sources on the prevention of desynchronization from flights and time zone changes in athletes are limited, which determines the relevance of the study. The purpose of this study is to determine the initial provisions of substantiation of preventive means of desynchronization in the practice of training athletes in game sports. Materials and methods of research. To determine the set research objectives, we used the following methods: analysis and generalization of data from special scientific and methodological lit erature, monitoring of Internet information resources, methods of systematization, content analysis, Sociological research methods (interview, questionnaire). Results. Based on available theoretical and methodological physiological principles and results of practical researches we made an attempt to substantiate the problem of desynchronization and the necessity of its prevention among sportsmen specializing in sports games. Conclusions. The obtained results made it possible to define and present in the article the basic bases of substantiation of preventive means of desynchronization in the practice of training of sportsmen in game sports.