Resource Bank Research Articles

Research on the Propagation Path of Red Literature Grounded Theory Studies Based on 27 Samples

Red is the most vivid background of the party's century struggle, red literature is an important carrier of red culture. In order to realize the red literature contemporary dissemination of the times, the popularity, the popularization. This research is based on a questionnaire survey of 27 readers to study the feasibility of red literature communication practice. The study found that the whole of the current social awareness of red literature reading promotion gradually increased, the promotion of the main body increasingly diverse, and the promotion of the form and content is increasingly rich, but at the same time, there is a lack of continuity and depth of extension activities. In the process of reading, readers are influenced by their own cultural literacy, social reading environment, literature theory is not easy to read and other problems to achieve its wide spread and the biggest obstacle to reading. Based on the actual situation, this paper puts forward some suggestions, such as building a red resource bank, enriching the forms of the external communication and innovating the forms of publication, etc., Starting from multiple angles, do a good job in the popularization and contemporary transformation of red literature to meet the diversified reading needs of the public.

The construction of University Chinese as a foreign language multimedia material and network resource base based on Big Data Analysis

There are many problems in TCFL, such as relatively simple teaching resources, chaotic teaching system and so on, facing learners from different countries and regions, cultural background and needs all over the world, directly affects the quality of college Chinese as a foreign language. With the development and application of Internet technology, online intelligent teaching has triggered profound changes in educational system. As an important supplement to education, web-based resource banks are of great practical significance to the current innovation and optimization of Chinese as a foreign language systems. Therefore, in the current innovation of University Chinese as a foreign language, we should build a large-scale and knowledge-based network resource base based on Big Data Analysis to help university teaching.

Research Progress on Illicium difengpi (Illiciaceae): A Review

Illicium difengpi K.I.B. et K.I.M. is a member of the Illiciaceae family with a yet not fully explored utilization value. To provide references for the systematic understanding of I. difengpi (Illiciaceae), the morphological and structural characteristics, wild resource distribution, chemical compounds, pharmacological effects, utilization, and protective measures of this species are reviewed. We conclude that (i) I. difengpi (Illiciaceae) is an endemic and indigenous medicinal species that has been used to treat rheumatoid arthritis and traumatic injury in China; (ii) I. difengpi (Illiciaceae) can endure various abiotic stresses, especially extreme drought, and thus has scientific value for exploring adaptive mechanisms of tolerance to extreme drought and in the ecological restoration of karst rocky desertification areas; and (iii) the beautiful tree shapes of I. difengpi (Illiciaceae) give it potential ornamental value. However, the wild resources of I. difengpi (Illiciaceae) have rapidly decreased, and there is an urgent need to protect this endangered species to maintain its diversity. Protection measures include the protection of wild germplasm resources, the establishment of an I. difengpi (Illiciaceae) germplasm resource bank, and the development of large-scale ecological planting techniques. In further research, the medicinal and scientific value of I. difengpi (Illiciaceae) should be systematically explored to clarify the plant’s effective pharmaceutical value, clinical applications, mechanisms of drought adaptation, and genetic diversity.

Embedding open and reproducible science into teaching: A bank of lesson plans and resources.

Embedding open and reproducible science into teaching: A bank of lesson plans and resources.

СТВОРЕННЯ БАНКУ СЕЛЕКЦІЙНОГО МАТЕРІАЛУ

Аn important issue of the selection process is the preservation of the source and obtained material, especially cross-pollinated crops, which significantly lose their viability by inbreeding. Creating a bank of genetic resources using biotechnological methods will effectively solve this problem. The aim of the work was to determine the conditions for the formation of a genetic bank of valuable winter rye materials with changes in temperature and modeling of the nutrient medium for long-term disposal of cloned plants and the use of active collection of original and created forms in the selection process. To deposit the clones, a nutrient medium, which included macro- and microelements according to the Murashige-Skuga medium was used. The nutrient substrate modified with cytokinins and carbohydrates. The clones in culture rooms at a temperature of 6–12 °С and low light intensity (2 kLk) were stored. In the course of research the conditions of creation of an active collection of plants of winter rye with use of temperature restriction and modification of a nutrient medium are defined. A consistent technological scheme for the conversion of plant material into a state of relative anabiosis has been developed. It is proved that the optimal storage temperature for samples is 6 °С. Survival of plants at the specified temperature regime after 12 months of deposition on average by genotypes at the level of 78,2 % was recorded. Modification of the nutrient medium with agar-agar at a concentration of 12,0 g/l increases the proportion of viable clones to 81,3 %, and the introduction into the substrate of an increased concentration of growth regulators, in particular 6-BAP (2,0 mg/l) and sucrose 40,0 g/l and a gradual decrease in temperature to 10 °С prolongs the period of deposition of cloned plants without changing the substrate and the shelf life of breeding material in isolated crops. Using of biotechnological methods for the preservation and reproduction of valuable material intensifies the selection process of obtaining initial samples of winter rye.

ANDRIY PETROVYCH KRUGLYAK – FAMOUS SCIENTIST AND PRACTITIONER IN THE FIELD OF SELECTION AND REPRODUCTION OF FARM ANIMALS

ANDRIY PETROVYCH KRUGLYAK – FAMOUS SCIENTIST AND PRACTITIONER IN THE FIELD OF SELECTION AND REPRODUCTION OF FARM ANIMALS

Energy and Resource Security: Some Insights

The import-export paradox emerges when countries, despite being rich in resources, are not willing to use their resources and in turn import them from other countries thus, depleting the other countries’ resources. As a case study, the mining sector in India provides evidence of the import-export paradox as despite being rich in minerals, India imports three times its own production of the same. The growing global demand of energy only puts more stress on this issue and thus it is crucial to address this paradox in order to achieve energy and resource security. One of the ways for doing this could be by establishing a resource bank that treats resources as capital and by doing so it ensures that the resources being used are also being replenished. Steps can also be taken at G20 level to develop a cooperative framework to address the same. At the Indian level, initiatives such as the International Solar Alliance can be strengthened by increased allocation of finances in order to build greater capacities.

Dry Storage of Bodily Fluids For Disease Diagnostics and Genome Resource Banking

Dry Storage of Bodily Fluids For Disease Diagnostics and Genome Resource Banking

Drawing and labelling: New ways of answering questions on the Assessment Resource Banks

Drawing and labelling: New ways of answering questions on the Assessment Resource Banks

Linking in‐situ and ex‐situ populations of threatened amphibians through genome banking

AbstractGenetic variation of the endangered Puerto Rican crested toad (PRCT; Peltophryne lemur) has dwindled over time in both wild and captive populations, leading to long‐term sustainability issues for the recovery program. To address this challenge, we propose that PRCTs can be used as a model species to show how in‐situ and ex‐situ populations can be linked through sperm biobanking and gamete transfer, expanding genetic variability of both populations. Male toads (n = 10) in Guayanilla, Puerto Rico were administered human chorionic‐gonadotropin (hCG) and luteinizing‐hormone‐releasing‐hormone analog (GnRHa) to stimulate spermiation. Sperm was collected noninvasively from 9/10 males, cryopreserved in 10% trehalose with either 10% N,N‐dimethylformamide (DMFA) or 10% dimethyl sulfoxide (DMSO), and transported in liquid nitrogen vapor to the National Amphibian Genome Resource Bank at Mississippi State University. Ultrasonography was used to identify females (n = 3) with mature oocytes for GnRHa‐induced oviposition of eggs for in‐vitro fertilization (IVF). Post‐thaw sperm motility was 28% and 25% for sperm cryopreserved with DMFA or DMSO, respectively. Of the 9,672 eggs used for IVF, 4% (n = 306/6,981) were fertilized with frozen–thawed sperm, compared with 20% (n = 525/2691) fertilized with fresh sperm controls. Overall, 46 toadlets were produced from frozen–thawed sperm. After 4.5 months of headstarting, 14 juvenile toads produced from various genetic crosses using frozen–thawed sperm were released to new sites in the wild, introducing unique genetic representation and new founder lines. After 1.5 years, 24 adult toads produced using frozen–thawed sperm continue to thrive in the captive collection, and one of these males has now produced an F2 generation of offspring with 5,085 tadpoles released to the wild. This transformational study is the first to produce reproductively viable adult PRCTs using cryopreserved sperm from hormonally‐induced wild males and captive females, introducing innovative methods that link in‐situ and ex‐situ populations of endangered amphibians to revolutionize genetic management.

Paeonol Attenuates Quorum-Sensing Regulated Virulence and Biofilm Formation in Pseudomonas aeruginosa.

With the prevalence of multidrug-resistant bacteria and clinical -acquired pathogenic infections, the development of quorum-sensing (QS) interfering agents is one of the most potential strategies to combat bacterial infections and antibiotic resistance. Chinese herbal medicines constitute a valuable bank of resources for the identification of QS inhibitors. Accordingly, in this research, some compounds were tested for QS inhibition using indicator strains. Paeonol is a phenolic compound, which can effectively reduce the production of violacein without affecting its growth in Chromobacterium violaceum ATCC 12472, indicating its excellent anti-QS activity. This study assessed the anti-biofilm activity of paeonol against Gram-negative pathogens and investigated the effect of paeonol on QS-regulated virulence factors in Pseudomonas aeruginosa. A Caenorhabditis elegans infection model was used to explore the anti-infection ability of paeonol in vivo. Paeonol exhibited an effective anti-biofilm activity against Gram-negative bacteria. The ability of paeonol to interfere with the AHL-mediated quorum sensing systems of P. aeruginosa was determined, found that it could attenuate biofilm formation, and synthesis of pyocyanin, protease, elastase, motility, and AHL signaling molecule in a concentration- and time-dependent manner. Moreover, paeonol could significantly downregulate the transcription level of the QS-related genes of P. aeruginosa including lasI/R, rhlI/R, pqs/mvfR, as well as mediated its virulence factors, lasA, lasB, rhlA, rhlC, phzA, phzM, phzH, and phzS. In vivo studies revealed that paeonol could reduce the pathogenicity of P. aeruginosa and enhance the survival rate of C. elegans, showing a moderate protective effect on C. elegans. Collectively, these findings suggest that paeonol attenuates bacterial virulence and infection of P. aeruginosa and that further research elucidating the anti-QS mechanism of this compound in vivo is warranted.

Research Progress of the Wild Medicinal Plant, Pinellia ternata

Based on literature reviews and analysis of research reports on Pinellia ternata found locally and abroad in recent years, this article summarizes and arranges them. The research on Pinellia ternata mainly focuses on its cultivation, tissue culture, and so on. There are only a few research on its active components and its regulation mechanism. The wild resources of Pinellia ternata are gradually decreasing, hence it is urgent to take effective measures to protect these wild resources as well as to establish germplasm resources bank and nursery. In order to meet the needs of the domestic market, it is necessary to investigate the distribution of wild Pinellia ternata resources, explore the best growing environment and conditions, artificially cultivate Pinellia ternata, as well as implement resource industrialization, sustainable development, and utilization.

Targeted capture sequencing identifies genetic variations of GRK4 and RDH8 in Han Chinese with essential hypertension in Xinjiang.

Essential hypertension is a common cardiovascular disease with complex etiology, closely related to genetic and environmental factors. The pathogenesis of hypertension involves alteration in vascular resistance caused by sympathetic nervous system (SNS) and renin angiotensin system (RAS). Susceptibility factors of hypertension vary with regions and ethnicities. In this study, we conducted target capture sequencing on 54 genes related to SNS and RAS derived from a collection of Han nationality, consisting of 151 hypertension patients and 65 normal subjects in Xinjiang, China. Six non-synonymous mutations related to hypertension were identified, including GRK4 rs1644731 and RDH8 rs1801058, Mutations are predicted to affect 3D conformation, force field, transmembrane domain and RNA secondary structure of corresponding genes. Based on protein interaction network and pathway enrichment, GRK4 is predicted to participate in hypertension by acting on dopaminergic synapse, together with interacting components. RDH8 is involved in vitamin A (retinol) metabolism and consequent biological processes related to hypertension. Thus, GRK4 and RDH8 may serve as susceptibility genes for hypertension. This finding provides new genetic evidence for elucidating risk factors of hypertension in Han nationality in Xinjiang, which in turn, enriches genetic resource bank of hypertension susceptibility genes.

Capparis Spinosa is an Alternative Drug for Vitality

From traditional and folk medicine, the fruit of Capparis used as antiseptic for intestinal dysentery and as protective for the liver from diseases in addition it is used as aphrodisiac and antihypertensive agent in addition as anticancer so as we know that natural product still a bank of new drug resources for the following reasons; these are a target for production by biotechnology. In addition, they are as a source of new lead compounds of novel chemical structure, which act as a tool for invention of new drug using nanoscince in medicine. There is a third reason as active ingredients of useful treatment divided from traditional medicine. As we know the drugs which manufacturing from bioactive, materials are cheap and available and not polluted as the chemical synthetic drugs and do not need-sophisticated technology. In this study we use fruits and leaves of Capparis dried and milled then extracted with alcohol 80% in addition deal with different organic solvent and get rid of chlorophyll and caryophilline and phytochemical studies by using liquid-liquid HPLC and we managed to extract quercitin and quercitrin in addition to proteins. The last product was anti-oxidant compared with racemic vitamin C using Noradrenaline as a test for the oxidation. Lastly, we see the activity of these materials as potent activator to the male compared with Sildenafil. The Capparis increase the activity twice the effect of sildenafil, And Tadalafil.

Addressing the Opioid Epidemic with a K-12 Drug Prevention Curriculum, Website and Toolkit

Opioid overdose has become a large public health crisis with devastating consequences, particularly for young people. Programs have started to address the impact of prevention and education in approaching the issue, but many lack focus on helping students prevent drug misuse by practicing the skills students need to avoid or reduce risky health behaviors. The purpose of this manuscript is to introduce readers to the process of developing skills-based K - 12 Opioid/Rx Use educational lessons. Procedures: The educational lessons were developed using data-driven practice for the following age level clusters; K-2, 3-5, 6-8, and 9-12. All lessons contain active learning strategies and include a resource bank such that any teacher, administrator, or school personnel can select and deliver a lesson using the online interface.

Post-Thaw Sperm Quality and Functionality in the Autochthonous Pig Breed Gochu Asturcelta.

Simple SummaryGenetic resource banks were created to preserve the genetic material of endangered, rare, valuable individuals or genetically relevant breeds. Sperm cryopreservation is a practical and widespread strategy to preserve these genetic materials. This study aimed to characterize the frozen-thawed sperm of the native pig breed Gochu Asturcelta, considering the effects of boar age and season of semen collection on post-thaw sperm quality. We found that the boar age did not have a significant effect on the sperm parameters assessed. However, the season significantly affected many of these parameters (motility, viability, acrosomal status, mitochondrial activity). In general, sperm samples collected in spring and summer showed higher quality post-thawing, the lowest in winter. Our findings demonstrated that the post-thawing sperm quality of Gochu Asturcelta was in the range of results for commercial breeds, bringing a good prospect for the use of assisted reproductive technologies in this local breed.Genetic resource banks (GRB) preserve the genetic material of endangered, valuable individuals or genetically relevant breeds. Semen cryopreservation is a crucial technique to reach these goals. Thus, we aimed to assess the sperm parameters of semen doses from the native pig breed Gochu Asturcelta stored at the GRB of Principado de Asturias (GRB-PA, Gijón, Spain), focusing on intrinsic and extrinsic (boar, season) factors. Two straws per boar (n = 18, 8–71 months of age) were thawed, pooled, and assessed after 30 and 150 min at 37 °C by CASA (computer-assisted sperm analysis system; motility and kinematic parameters) and flow cytometry (viability, acrosomal status, mitochondrial activity, apoptosis, reactive oxygen species, and chromatin status). The effects of age, incubation, and season on post-thawing quality were determined using linear mixed-effects models. Parameters were on the range for commercial boar breeds, with chromatin status (SCSA: fragmentation and immaturity) being excellent. Incubation decreased sperm quality and functionality. The boar age did not have a significant effect (p > 0.05), but the between-boar variability was significant (p < 0.001). The season significantly affected many parameters (motility, kinematics, viability, acrosomal status, mitochondrial activity), especially after 150 min of incubation. In general, samples collected in spring and summer showed higher quality post-thawing, the lowest in winter. In conclusion, the sperm doses from the Gochu Asturcelta breed stored at the GRB-PA showed excellent chromatin status and acceptable characteristics after thawing. Therefore, boar and seasonal variability in this autochthonous breed could be relevant for cryobank management.

Effects of somatic tissue cryopreservation on puma (Puma concolor L, 1771) tissue integrity and cell preservation after in vitro culture

Somatic resource banks play a crucial role in the conservation of genetic diversity, allowing for the preservation of biological samples from different populations. Puma somatic cells can be recovered from these banks and used in assisted techniques toward enhancing their multiplication and conservation. In response to the population reduction of this ecologically importance species, we aimed to evaluate the capacity of cryopreservation of somatic tissues on the maintenance of the integrity and quality of the cells recovered after culture, with the aim of establishing a somatic tissue bank that will allow for the safeguarding of a wide genetic sampling of pumas. Cryopreservation increased the thickness of the corneum layer in the tissues, and the number of perinuclear halos and empty gaps. Nevertheless, cryopreservation was able to maintain normal fibroblast patterns, even showing an increase in the percentage of collagen fibers. Cryopreservation maintained the proliferative potential of the tissues and the parameters evaluated during in vitro culture, mainly regarding the viability, proliferative activity, and apoptosis levels. Nevertheless, cells from cryopreserved tissues showed decreased metabolism and mitochondrial membrane potential when compared to cells from non-cryopreserved tissues. In summary, we demonstrated for the first time that puma somatic tissues subjected to cryopreservation are viable and maintain tissue integrity, featuring minimal changes after warming. Although viable somatic cells are obtained from these tissues, they undergo alterations in their metabolism and mitochondrial membrane potential. Improvements in the conservation conditions of somatic samples are needed to increase the quality of somatic tissue banks in this species.

English

Myxobacteria can produce rich and varied biological active substances against bacteria, fungi and viruses, which have great development and research value in medicine and agriculture. In this study, the diversity of culturable myxobacteria in central Inner Mongolia in China was studied and the effects of soil utilization mode, soil type and environmental parameters on the distribution of myxobacteria in this region were analyzed. Furthermore, the activities of myxobacteria against potato late blight pathogen were tested. The results showed that Myxococcus, Corallococcus, Pyxidicoccus, Cystobacter, Archangium and Mellittangium were the dominant genera of myxobacteria in this region. Soil utilization mode and soil types have obvious influence on the distribution of myxobacteria. The populations of myxobacteria were abundant in grassland and cultivated land samples, but few in woodland and unused land samples. The diversity of myxobacteria in the soil samples from fuvo-aquic soils, grey-cinnamon soils, castanozems, and bog soils was relatively rich, while the richness of myxobacteria in aeolian soils, solonetzs, skeletol soils and castano-cinnamon soils was poor. There was no significant correlation between myxobacteria distribution and soil environmental parameters (including the water content, pH value, content of organic matter, content of available phosphorus, content of hydrolytic nitrogen and content of available potassium). Most of the myxobacterial strains isolated in this area (83%) showed the activity against P. infestans, among which the proportion of the disease-resistant strains belonging to Myxococcus and Corallococcus was high, the proportion of the strains belonging to Cystobacter and Mellittangium in the medium, and the proportion of the strains belonging to Pyxidicoccus and Archangium low. The completion of this work will enrich the myxobacteria resource bank in Inner Mongolia and lay a foundation for the further study on myxobacteria and the development of biological pesticide against potato late blight. © 2021 Friends Science Publishers

Integration of Information Technology and PE Teaching Process

Since entering the new era, with the rapid development of information technology and the acceleration of the process of global economic integration, many aspects of my country’s social life and economic fields are undergoing profound historical changes. The integration of information technology and physical education is becoming a hot topic in my country’s current information technology education and even the entire education informatization process. The purpose of this article is to study the integration of information technology and physical education process. This article uses modern information technology to integrate with physical education. The teaching effect produced by it is significantly better than the traditional teaching model. It can more effectively solve the difficulties in the textbook, and can help students establish movement representations and understand movements faster. Essentials to stimulate students’ interest in learning. This research systematically elaborates the connotation and significance of information technology and the impact of information technology on middle school physical education, and scientifically explores the physical education curriculum, analyzes the basis and conditions of the integration of physical education and information technology education, and studies the information technology and middle school through teaching experiments. The mode and effect of physical education integration. This article takes the university’s physical education major curriculum integration as the research object, and conducts research and analysis through literature method, questionnaire survey method and mathematical statistics method to define the level of information technology and physical education major curriculum integration. Investigation and research show that more than 30% of physical education humanities teachers can often find a suitable resource bank and obtain relevant teaching materials, but the lack of targeted physical education software largely affects teachers’ access to resources.

A place to share teaching resources: Speech and language resource bank

One of the major difficulties of teaching is accessing the vast amounts of material/resources already available to instructors. The Speech and Language Resource Bank (SLRB) is a platform and central repository for sharing, managing, and growing community resources for teaching and research in the speech and language sciences.