Resorption Of Bone Tissue Research Articles

Triad of female athletes

The female sports triad is a spectrum of dysfunctions related to energy availability, menstrual function, and bone mineral density. Energy availability is considered the trigger for the triad. Scientists have proven that women who do sports consume approximately 30% less energy and carbohydrates in relation to their body weight. Sufficient energy availability ensures the presence of a menstrual cycle, directly affects the stimulation of the production of female sex hormones (estrogen in particular). Thus, it prevents resorption of bone tissue. When energy availability is too low, a physiological mechanism reduces the amount of energy used for cell maintenance, thermoregulation, growth, and reproductive function. This compensation tends to restore energy balance and promotes survival, but impairs health. Low body weight in women of reproductive age, particularly in adolescence and the first reproductive age, is a biological marker of somatic and reproductive dysfunction. Osteoporosis in young female athletes is manifested by fractures unusual for young women or by frequent and multiple bone fractures during an athletic career. In this review article, an analytical review of literary and informational sources regarding the history of research on the problem of the triad of female athletes is carried out. The specifics of the negative impact of the athlete triad on the female body, the components of the athlete triad (amenorrhea, eating disorders, and osteoporosis) are examined in detail. The article focuses on its main cause - energy imbalance. The impact of the triad of female athletes on the psyche and behavior is considered. The question of its consequences for health both in the short-term and in the long-term perspective is considered. The prevention of RED-S syndrome was also investigated. The purpose of the study: Systematization of current knowledge about the triad of female athletes (relative energy deficiency syndrome (RED-S)). Research methods. Theoretical analysis of literary sources, systematization, generalization of scientific-methodical and special literature.

Stress-strain state of an intraosseous dental implant under stress under unfavorable biomechanical conditions

Biomechanical conditions affect the stress-strain state of teeth, dentures, implants and surrounding tissues, however, some biomechanical processes in implants and covering prosthetic structures have not been sufficiently studied under different operating conditions. The purpose of the study. А comparative analysis of the maximum stresses in titanium implants, abutments and covering ceramic crowns under different biomechanical operating conditions. Material and methods. Three-dimensional mathematical modeling of the stress-strain state of the implant, abutment, crown was carried out using the SolidWork computer program in a model of an implant immersed in a segment of the mandible and covered with a ceramic crown. Load conditions: vertical or inclined 45° with a value of 150 N. Well-known indicators of the physical and mechanical properties of fabrics and structural materials were used. Adverse biomechanical conditions were simulated: a 30% reduction in the length, diameter, length and diameter of the implant; an abutment slope of 15° and 30°; bone resorption by 30% and 50%; increased load by 30%; occlusive supracontact; contact with bone tissue by 50% (immediate load). Results and discussions. In comparison with optimal biomechanical conditions, the stresses in the implant and the covering crown increase under vertical and inclined loads only with an increase in load and the presence of occlusive supracontact. An increase in stresses only in the implant occurs with a decrease in the diameter of the implant, a combined decrease in the diameter and length of the implant, with an abutment tilt, resorption of periimplant bone tissue, incomplete contact of the implant with bone tissue under immediate load. Stress reduction in the implant and the covering crown occurs when the length of the implant decreases. Conclusions. Unfavorable biomechanical conditions in most cases lead to an increase in stresses in the implant, especially with inclined loading; the decrease in stresses in the implant with a decrease in the length of the implant is most likely due to an increase in stresses in the surrounding bone tissue.

Combined use of PRPP, diode laser and piezosurgery device improves reparative osteogenesis previously to dental implants placement

ObjectiveCurrent study evaluated whether use of the platelet-rich powder (PRPP), piezosurgery device and diode laser after removal of jawbones cysts and benign bone formations and extraction of impacted tooth could enhance bone regeneration before dental implantation. MethodsA study was conducted among 200 patients with post-surgical cavities (not exceeding 1.5 cm upon cystectomy, benign bone tumor removal and impacted tooth extraction) who underwent plasma powder, autograft, allograft, xenograft or beta-tricalcium phosphate augmentation procedures. ResultsPRPP implantation in combination with irradiation of bone cavity by diode laser led to the formation of 15.40 % ± 2.1 of new bone. The activity of reparative osteogenesis on the bone surface was BB = 6.71 ± 1.3, which is 3 and 3.5 times higher than the mean value in comparison group after augmentation of xenograft and beta-tricalcium phosphate. The value of bone tissue resorption for main group patients was less pronounced (Ra.Oc = 25.20 % ± 2.1).The ratio between pro- and anti-inflammatory cytokines in the main group after 1, 7 and 30 days since surgical procedure was lower than the ratio of the corresponding cytokines in the comparison groups. ConclusionThe examination of computed tomograms, histological and morphometric analysis of bone tissue after trepanobiopsy, cytological analysis of swabs and enzyme immunoassay to determine local immunity indicated that the proposed method of treatment with the use of PRPP, the piezosurgery device and the diode laser has a positive effect: it helped to optimize reparative osteogenesis after filling post-surgical cavities and restore bone volume.

Comparative Effectiveness of an Autologous Dentin Matrix for Alveolar Ridge Preservation.

An urgent issue is the preservation or reconstruction of the volume of bone tissue in planning and surgical treatment in the fields of medicine, such as traumatology, orthopedics, maxillofacial surgery and dentistry. After tooth extraction, resorption of the bone tissue of the alveolar crest of the jaws occurs, which must either be further eliminated by performing additional operations or using osteoplastic material for socket preservation at the extraction stage. Background and Objectives: The aim of the study was a comparative analysis of various osteoplastic materials used to preserve the volume of bone tissue in the preimplantation period. Materials and Methods: As part of the study, 80 patients were treated, who underwent socket preservation using xenografts, plasma enriched with growth factors, an autologous dentin matrix (ADM) and hydroxyapatite. Results: The results of the treatment 16 weeks after removal were comprehensively analyzed using a morphometric analysis of the bone's volume, cone beam tomography and morphological examination of burr biopsy specimens, as well as by determining the stability of the installed implant at different stages of treatment. Conclusions: The lowest level of bone tissue resorption according to the CBCT data was noted in the ADM and xenograft groups. It should be noted that the use of osteoplastic material in jaw surgery when reconstructing alveolar defects is an essential procedure for preventing the atrophy of bone tissue.

Aspects of the dynamics of osteogenesis and bone tissue resorption markers concentrations in elite biathlonists due to the compression specificity of the cyclic training aids used at the stages of the early season

Aim: study the dynamics of osteogenesis and bone resorption markers in elite biathlonists owing to the “compression” specifics of cyclic training aids, mainly used at the stages of the early season of the annual training cycle.Materials and methods: 23 elite biathlonists undergoing centralized training as the members of the Russian national team took part in the study.Results: no changes in the P1NP amounts reaching a reliable level were recorded throughout the early season. The average group osteocalcin amount at the precompetition stage significantly (p < 0,05) increased compared with that measured at the special preliminary stage. Dynamics of β-CrossLaps concentration showed significant (p < 0,05) decrease during the transition from the general preliminary to the special preliminary stage.Conclusion: the degree of intense training activity influence on the activity of bone cells and in turn the response of bone tissue to training loads depend on a set of exercise parameters performed by the athlete, such as duration, intensity, specificity, recovery time and mechanical stress. Optimizing the ratio of cyclic aids with different levels of compression loads during specialized training promotes adaptive changes which protect bone tissue from resorption affected by intense physical exercises. When analyzing the levels of bone metabolism markers for diagnosis and monitoring disorders associated with skeletal overload which could occur high-intensity training loads, an individual approach as well as recognition of the load specifics and parameters performed by an athlete at a specific stage of the training macrocycle are required.

Histomorphological assessment of the germanium-doped calcium phosphate ceramics on reparative osteogenesis in rabbits with systemic osteoporosis

Bone fractures are mostly urgent in nature, complex in pathogenetic, diagnostic and therapeutic aspects, and reparative osteogenesis is multimodal and depends on the balanced and reciprocal interaction of many factors. The results of histological studies for osteoreplacement of bone defects in rabbits with systemic osteoporosis are presented. The purpose of the work is histomorphological evaluation of bone regenerates after osteoreplacement with germanium-doped hydroxyapatite ceramics in rabbits with secondary osteoporosis. Experimental osteoporosis in rabbits (n=18) was induced by administration of 0.4% dexamethasone solution. In animals of the experimental group, bone defects were replaced with granules of hydroxyapatite ceramics doped with germanium, and in animals of the control group, they healed under a blood clot. Histological sections were made on a rotary microtome with a thickness of 5 to 10 μm and stained with Weigert's iron hematoxylin and 1% alcohol-based eosin solution (manufactured by Diapath, Italy). On the 60th day of reparative osteogenesis in the animals of the experimental group, the site of the bone defect was flled with compact bone tissue with minor remnants of spongy bone tissue. Slightly expanded Haversian canals were noted. In the control group, the defect site was flled with coarse and spongy bone tissue. Bone beams of various thicknesses with a small number of osteoblasts and single walled osteocytes were visualized. A signifcant number of osteocyte lacunae were empty due to the process of osteocyte lysis. Haversian canals are signifcantly dilated with a small number of vessels. Also, lacunae of bone tissue resorption and acellular areas were observed on histological specimens. Histomorphological assessment of bone regenerates confrms the realization of osteoconductive, osteointegrative and osteoinductive properties of calcium-phosphate ceramics doped with germanium in conditions of osteoporotic fractures of tubular bones. Keywords: systemic osteoporosis, bone fractures, cancellous and compact bone tissue, histological sections, histomorphological changes, rabbits.

OSTEOGENESIS AND BONE RESORPTION IN PATIENTS WITH POSTOPERATIVE MANDIBULAR BONE DEFECTS DURING INFLAMMATORY PROCESSES

Tooth extraction has long been the most common surgical procedure. Preserving the height of the alveolar ridge after extraction is crucial for further replacement of the missing tooth, including through dental implantation. This study aimed to assess the effect of cryopreserved placenta and quercetin on bone formation and osteoresorption markers in oral fluid. Ninety patients who underwent lower tooth extraction due to inflammation participated in the research. They were divided into three clinical groups of 30 each: the first group received cryopreserved placenta after extraction, the second group received combined therapy of cryopreserved placenta and quercetin, and the third (control) group underwent standard tooth extraction. Osteocalcin and matrix metalloproteinase-8 levels in the oral fluid were measured on days 1, 10, 20, 30, 90, and 180 after extraction for all groups. Antagonism between "osteocalcin" and "matrix metalloproteinase-8" was observed in the oral fluid during reparative osteogenesis. However, statistically significant changes in this marker were noted earlier in the observation period and were more pronounced, possibly due to local changes predominating. This suggests activation of bone tissue resorption processes in the early stages of the study and osteogenesis in the later stages of observation. The least dynamic changes in the studied indicators were observed when cryopreserved placenta was used in combination with quercetin treatment.

Evaluation of anti-inflammatory properties on the surface of dental implants depending on the type of processing (Part 1)

The aim. To study of the anti-inflammatory properties of the surface of commercial dental implants with different processing methods using the example of the course of the first stage of implantation. Materials and methods. Dental implants made of titanium with 3D Active and Xpeed surface treatment were used. The surface microstructure and biocompatibility of the samples, surface corrosion, elemental analysis of the surface was studied. The assessment of the severity of peri-implantitis and mucositis based on the depth of the peri-implant pocket and bleeding during probing, determination of the coefficient of stability of implants (KSI) is given. Median test (χ2), Kruskel–Wallis test (H), univariate variance analysis (F) were used for multiple comparison of samples. The difference in parameters was considered statistically significant at the p ≤ 0.05 level. Results. The surfaces of 3D Active implants are monolithic with rounded pores in the form of wells of different diameters and depths; the upper layer of the Xpeed sample consists of closely adjacent to each other fragments of a rectangular or polygonal shape, in the form of tiles, leaving rectangular faces on the outside. On the surface of the 3D Active samples, in addition to oxygen and carbon, there was phosphorus – 5.04 wt%, as well as S, Ca, Na, Zr, Zn which weight percentage did not exceed 1 wt%. A significant difference in the elemental composition of Xpeed implants was the presence of chlorine 0.07 wt% and 0.93 wt%, silicon – 0.10 wt% and 0.14 wt%, aluminum 0.06 wt% and 0.23 wt%, respectively, with the latter has a significant advantage in these elements. Xpeed had an insignificant weight percentage of iron in its composition – 0.12 wt%. The assessment of cell adhesion to the surface of the samples on the first day of incubation demonstrated that the samples with the Xpeed and 3D Active surfaces did not have a statistically significant difference between them. Also, on the 7th day of incubation, the level of reduction of resazurin in Xpeed was lower, no significant difference was observed (p > 0.05). The clinical signs of bone tissue resorption were identified in 36.4 % of 3D Active implants and 60.0 % of Xpeed implants (p = 0.3); inflammation of the mucous membrane – in the areas of installation of 54.5 % of 3D Active implants. No signs of mucositis were detected around the Xpeed implants (p = 0.23). 9.1 % of 3D Active implants were “lost”; no cases of loss of Xpeed implants have been identified. The KSI of 3D Active – 64.0 ± 5.9 units, Xpeed – 65.1 ± 3.7 units. Conclusions. According to KSI indicators, titanium implants with Xpeed coating (65.1 ± 3.7 units) and 3D Active (64.0 ± 5.9 units) showed the same capabilities. Bone tissue resorption without signs of inflammation is more common around Xpeed-coated implants than 3D Active-coated implants. Clinical signs of inflammation are more frequent and more severe around implants with a 3D Active coating, which affected both the severity of the course of the first stage of implantation and its results. Xpeed-coated implants are more reliable than 3D Active: loss among 3D Active implants was 9.1 %, Xpeed – 0.0 %.

Influence of the angle of dental implant placement on the stress-strain state of the jaw

prosthetics on dental implants is a complex and responsible stage of treatment of patients with dental defects. The success of dental implantation depends not only on careful planning and performed surgical intervention in compliance with the requirements of the protocol, but also on the type of load on the dental implant, the choice of orthopedic design and material. Thorough preparation for prosthetics, taking into account the initial clinical situation and individual characteristics of the patient, ensures a full restoration of the dentition defect with reproduction of the chewing function. The parameters of the stress-strain state in the bone tissue around dental implants are of the greatest importance for the effectiveness of prosthetics on implants, especially in the long term of their functioning. Significant power loads of variable amplitude in the jaw lead to resorption of bone tissue and failure of implants as artificial dental prostheses. In this regard, research on modeling the stress-strain state in bone tissue under different loading conditions of implants is relevant. This research presents the results of studying the stress-strain state of the bone-implant system under static loading. Numerical calculations were performed for four installation angles of the implant: 0°, 7°, 15°, and 22°. The solid model of the mandible and the implant model were made in Spaceclaim software, and the stresses were determined using ANSYS Workbench. The results obtained show the effect of the implant placement angle on the distribution of stress fields in the bone tissue and implant. The analysis of the calculation results will help to ensure optimal functioning and durability of dental implants.

MORPHOLOGICAL CHANGES OF RAT PERIODONTIUM IN SUPRAOCCLUSAL RELATIONS OF INDIVIDUAL TEETH IN THE AGE ASPECT

Although the issue of the occlusal load influence on the occurrence of pathological phenomena in the body has been discussed for decades, it is still a subject of debate. Pathogenic occlusion is defined as excessive occlusal forces (premature contacts and interference) that can cause injuries to the dentition, including teeth and periodontal tissues. Changes in occlusal dimensions, if not properly performed or treated, can lead to dysfunctions of the dentition, such as joint clicking, difficulty in chewing, occlusal discomfort, muscle fatigue, and pain.
 Aim: to determine the morphological changes of periodontium in rats of different age groups in the supraocclusal relationship of individual teeth in the age aspect.
 Materials and methods of the study. The study was conducted on 60 white laboratory rats aged 4 to 22 months and weighing 90 to 330 g. The rats were divided into control (30 animals) and experimental (30 animals) groups. Each group was divided into 3 subgroups (10 animals each) depending on the age of the rats: young, mature and senile. Young animals included rats aged 3 4 months with a weight of 90 100 g; mature animals aged 6-8 months with a weight of 150 170 g; senile rats 20 22 months and weighing 200 220 g. In the experimental group, the state of supraocclusion was modeled by increasing the height of the lower right second molars by placing 1 mm high filling. For the placement of the filling, 37% orthophosphorus etching gel for enamel and dentin (Latus, Ukraine), microapplicators (Latus, Ukraine), the fifth generation Prime&Bond NT adhesive system (Dentsply Sirona, Germany), the universal microhybrid light-curing composite material Latelux (Latus, Ukraine) and the photopolymerizer LED.D (WOODPECKER, China) were used according to the manufacturer's instructions. The experimental animals were withdrawn from the experiment on the 15th day by decapitation under general anesthesia with sodium thiopental. The lower jaws were skeletonized, and dental blocks were cut out in the area of the molars. For histological examination, the dental blocks were fixed in a 10% solution of neutral formalin for two days, dehydrated in alcohols of increasing concentration and embedded in paraffin. Then, sections were made from the obtained preparations on a Shandon Finesse 325 rotary microtome with a section thickness of 4-6 μm. The histological sections were fixed on degreased slides and dried. Van Gieson staining was performed. The width of the periodontal gap was determined using the ZEISS ZEN 3.7 program by Carl Zeiss Microscopy.
 Results of the study. Histometric analysis has showed significant morphological changes in the experimental group of all age categories. In young rats, the fibrinoid edema phenomena of the periodontal ligament collagen fibers were observed. In mature rats, there was a picture of mucoid edema of collagen fibers of the periodontal ligament. In senile rats, fibrinoid necrosis of collagen fibers of the periodontal ligament were discovered, an inflammatory infiltrate, macrophages and neutrophils, with a predominance of neutrophilic inflammatory microenvironment, and initial hyaline tissue changes were found. In all age groups, there was cement resorption on the lateral surfaces of the tooth root, lacunar resorption of alveolar bone tissue and the presence of a large number of osteoclasts. In senile rats, hypercementosis was determined in the apical part of the root. In the control group of all age categories, neither resorption nor disorganization of connective tissue, nor hypercementosis was detected.
 Conclusions. The study shows that traumatic occlusion leads to resorption of root and alveolar cementum in all age groups. In young rats, traumatic occlusion causes fibrinoid edema of collagen fibers of the periodontal ligament. In mature rats, traumatic occlusion causes mucoid edema of collagen fibers of the periodontal ligament. In senile rats, traumatic occlusion causes fibrinoid necrosis of collagen fibers of the periodontal ligament, initial hyaline tissue changes and hypercementosis.

Relationship in development of malocclusions to polymorphisms of selected vitamin D receptors.

The development of malocclusion is related to various factor, many of which are still not fully explained. The steroid hormone, 1,25-dihydroxyvitamin D3, has pleiotropic effects. It plays a key role in skeletal metabolism and the control of cell repair by attaching to the nuclear vitamin D steroid receptor (VDR). This vitamin affects bone turnover through the processes of bone tissue formation and resorption via its action on cells of the osteoblastic and osteoclastic lineage, exerts a modulating effect on the immune system, and is involved in the regulation of cell proliferation and differentiation. The role of vitamin D3 (VD3) and its receptor polymorphisms is a rarely studied topic in dentistry. Due to the proven influence on bone turnover processes and immune responses, the main research topic is its relation to periodontal diseases, but so far, its role in the formation and development of malocclusions has not been assessed. This study aimed to assess the association of selected VDR polymorphisms: Cdx2 (rs11658820), TaqI (rs7975232), BsmI (rs1544410), ApaI (rs7975232), and FokI (rs2228570) with the development of malocclusions. A prospective observational study was performed. The examination consisted of a medical interview, intraand extraoral orthodontic diagnosis, alginate impression, cone beam computed tomography (CBCT), and venous blood sample to obtain genomic DNA and assess VDR polymorphisms. The rs11658820 polymorphism causes an almost 4-fold increase in the probability of the presence of a malocclusion. GT and TT genotypes of rs7975232 are also associated with a similar risk - almost 6 and almost 5 times higher, respectively. In turn, the effect of the rs2228570-AG and GG genotype polymorphisms on the occurrence of transversal anomalies was demonstrated (odds ratio (OR) = 8.46 and OR = 6.92, respectively). The association of individual polymorphisms with specific malocclusions should be carefully assessed, especially since some trends have been indicated.

CHANGES IN THE WIDTH OF THE PERIODONTAL GAP OF RATS WITH SUPRAOCCLUSAL RELATIONSHIPS OF INDIVIDUAL TEETH IN THE AGE ASPECT

Introduction. The dentoalveolar apparatus is a complex of complicated interaction of the jaws, masticatory muscles, teeth, and temporomandibular joints. This connection is carried out by the trigeminal nerve system with sensory and motor nuclei closely connected to the cortical and subcortical centers of the brain. Occlusal trauma is a lesion that develops in the periodontium as a result of forces that exceed the periodontal potential for functional adaptation. Occlusal trauma can be classified as primary or secondary. The first one occurs in the pulp and periodontal ligament when the force of the action on the dental structures is greater than usual, under optimal periodontal support conditions. It includes orthodontic movements, "high" restorations, and parafunctional habits such as bruxism. Secondary occlusal trauma occurs when the teeth with previous supporting tissue changes are subjected to the action of normal and excessive forces with supporting tissue changes caused by periodontal disease or previous trauma. The available evidence has demonstrated that bacterial plaque bioactive substances lead to local inflammatory responses in periodontal tissues which contribute to bone tissue resorption through osteoclasts in periodontitis. Excessive functional stress can initiate inflammatory changes in the periodontium and thus, increase destructive bacterial processes. Traumatic occlusion can expand the space of the periodontal ligament and in this way stimulate apical bacterial migration and proliferation. However, few attempts have been made to investigate the molecular mechanisms that trigger bone tissue resorption in traumatic occlusion. The analysis of publications on the studies of morphological changes in periodontal tissues in individual teeth supraocclusion in the age aspect shows the contradictory data. The aim: to determine the morphometric changes of the periodontal gap as a component of the periodontal complex in rats of different age groups in the supraocclusal relationship of individual teeth in the age aspect. Materials and methods of the study. The scientific experiment was conducted on 60 white laboratory rats aged 4 to 22 months and weighing 90 to 330 g. The rats were divided into control (30 animals) and experimental (30 animals) groups. Each group was divided into 3 subgroups (10 animals each) depending on the age of the rats: young, mature and senile. Young animals included rats aged 3–4 months with a weight of 90–100 g; mature animals - aged 6–8 months with a weight of 150–170 g; senile rats – 20–22 months and weighing 200–220 g. The method of modelling the state of supraocclusal relations of individual teeth is described in the previous publication. For histological examination of dental blocks, the following was performed. Fixation in a 10% solution of neutral formalin for two days, dehydration in alcohols of increasing concentration and embedding in paraffin. After preparation, sections were made on a Shandon Finesse 325 rotary microtome with a section thickness of 4-6 µm. The histological sections were fixed on pre-degreased slides and dried. Van Gieson staining was performed. The width of the periodontal gap was determined using the ZEISS ZEN 3.7 program by Carl Zeiss Microscopy. For statistical data processing, the AtteStat v.12.5 software was used to determine the mean value and its standard error (M±m). The probability of a statistically significant difference between the data obtained was assessed using the Student's parametric test (at a significance level of p ≤ 0.05). All samples were tested for normality using the Gupta test. Results of the study. Statistical differences were observed in rats of all groups, as well as between the indicators of all rats of the control and experimental groups (≤0.05). The width of the periodontal gap increased by 34.1% in young rats, by 25.5% in mature rats, and by 21.1% in senile rats. Conclusions The presence of supraocclusal relationships of individual teeth causes an increase in the periodontal gap in rats of all age groups. The greatest expansion of the periodontal gap has occurred in young rats. The width of the periodontal gap in rats decreases with age.

ORTHOPEDIC REHABILITATION OF PATIENTS WITH CHRONIC GENERALIZED PERIODONTISIS USING DENTAL IMPLANTS

One of the most common methods of correcting dental defects used in modern dentistry is dental implantation. However, in conditions of periodontal pathology, planning and carrying out dental implantation causes certain difficulties. The aim of the work was to analyze the clinical effectiveness of orthopedic rehabilitation based on implants in patients with chronic periodontitis. The task of the study was to study and compare the indicators of implant stability, marginal bone tissue loss and postoperative complications in patients with chronic generalized periodontitis under the conditions of using the technique of immediate and delayed dental implantation.
 The object and methods of research. To achieve the goal, the authors formed two observation groups, in which the replacement of tooth row defects with the help of dental implants was performed in patients with generalized periodontitis of the I-II degree of severity. The first group consisted of 10 patients who underwent direct implantation and immediate functional loading. There were 10 patients in the second group, after delayed implantation, loading occurred in the generally accepted terms (2-3 months on the lower jaw and 4-5 months on the upper).
 The stability of the implants was determined using the Osstell ISQ device. A comparison of the indicators of implant stability with different implantation protocols and functional load: immediate and delayed load was carried out.
 Conclusion. The maximum value of stability of implants is registered after one year. This fact is proven in a statistically significant increase (p<0.05) in the stability of the implant in both groups.X-ray evaluation of bone tissue resorption in the cervical area after the first year of loading indicates certain risks of direct implantation and immediate functional loading.
 Thus, effective prosthetics for patients with generalized periodontitis is determined by a personalized approach to each individual case, taking into account local factors and individual characteristics of the body. Most often, it is unsatisfactory oral hygiene that leads to an increase in the amount of dental deposits and creates prerequisites for the exacerbation of the inflammatory-dystrophic process in the periodontal tissues.

EXPERIENCE IN APPLYING OSTEOPLASTIC MATERIALS CONTAINING MULTIPOTENT MESENCHYMAL STROMAL CELLS AND HYDROXYAPATITE IN PATIENTS BEFORE THE PROCEDURE OF DENTAL IMPLANT PLACEMENT

Introduction of modern osteoplastic material into practical work is extremely important for effective filling of bone defects, restoration of lost periodontal tissue, relief of postoperative symptoms and reduction of inpatient treatment of patients after surgery. Up-to-date materials will improve regenerative and reparative properties of the bone tissue and promote its increasing volume for further successful dental implantation. Objective of the research was to determine an application effect of osteoplastic materials based on multipotent mesenchymal stromal cells of the adipose tissue and hydroxyapatite used for filling bone defects during dental surgery in patients, before dental implantation. Materials and methods. 140 patients were examined and treated by means of dental implantation. Before implantation, in order to increase the volume of the bone tissue of the cellular processes and cellular part of the corresponding jaw, various osteoplastic materials were applied during surgery. All the patients were distributed into III groups: І included 49 individuals (35,0 %), II group – 62 patients (44,29 %), and III (control) group included 29 patients (20,71 %). In I group a bone tissue substitute based on hydroxyapatite was used to restore the bone tissue of the cellular processes and the cellular pert of the corresponding jaw. In II group augmentation of bone defects was carried out by means of the suggested composition consisting of multipotent mesenchymal stromal cells of the adipose tissue, platelet-rich blood plasma and bone tissue substitute based on hydroxyapatite. In ІІІ group, the wound healed spontaneously under a blood clot. Results. After installation of dental implants, the peri-implant tissues were found to restore completely in cases where the combination of multipotent mesenchymal stromal cells of the adipose tissue, a bone substitute based on hydroxyapatite, and platelet-rich blood plasma was used for filling bone defects. Moreover, according to X-ray findings obtained 12 months later, in patients from II group the volume of the bone tissue resorption in the areas of implantation was 1,4 and 1,8 times lower than in individuals from І group, р1<0,01, and ІІІ control group, р2<0,01, respectively. Conclusions. The effectiveness of application of osteoplastic materials based on multipotent mesenchymal stromal cells of the adipose tissue and hydroxyapatite is confirmed by the values of the stability coefficient of implants. After 12 months of the research, these values were reliably higher than those of the reference, р<0,01. They were 12,43 % and 19,33 % higher than in patients from І main group and ІІІ control group.

Assessment of indicators of bone tissue resorption in patients with chronic generalized periodontitis according to the data of cone-beam computer tomography

Assessment of indicators of bone tissue resorption in patients with chronic generalized periodontitis according to the data of cone-beam computer tomography

Comparing the Healing Abilities of Fluorapatite and Hydroxyapatite Ceramics in Regenerating Bone Tissue: An In Vivo Study.

Some reports in the literature show the advantages of fluoride-containing apatite ceramics over hydroxyapatite (HAP), at least in some aspects. While HAP has been used extensively in the treatment of bone defects, fluoridated apatite has hardly been tested in vivo. In order to verify the biological properties of fluoride-doped apatite and to assess its therapeutic potential, we synthesized fluorapatite (FAP) and applied it as a filling in bone defects of experimental animals (rabbits). The treatment effects were evaluated on extracted bones after 3 and 6 months from implantation using peripheral quantitative computed tomography (pQCT), dual-energy X-ray absorptiometry (DXA), radiography (X-ray) and histological staining. The study proved the integration between FAP and the bone tissue, thus indicating its stimulating effect on new bone formation and mineralization. The results achieved after 3 months of treatment were difficult to interpret unequivocally and suggested the transient delay in FAP integration of bone in comparison with HAP. The reasons for this phenomenon are unclear. Most likely, these differences between FAP and HAP resulted mainly from the different porosities, densities and ionic reactivity of the ceramics, which in our opinion affected their solubility, integration and degree of bone tissue resorption. However, it was shown that 6 months after implantation, similar level of bone defect regeneration was achieved for both FAP and HAP. In this article, we present our hypothesis concerning the basis of this phenomenon.

Clinical masks of marble disease

Marble disease, or osteopetrosis (OPT), is rare in the practice of a rheumatologist, internist or pediatrician. This group of hereditary diseases is based on a defect in the formation, development and functioning of osteoclasts (OCL), which leads to disruption of the processes of resorption and remodeling of bone tissue. Disturbance of resorption leads to increased density and changes in the quality of the bones, as a result of which they become more fragile. As a rule, the disease manifests with pathological fractures. In recent decades, 70% of patients with OPT have been found to have mutations in at least 10 genes that lead to impaired functioning of the OCL. Depending on the variant of inheritance, autosomal dominant, autosomal recessive and intermediate types of OPT are distinguished. Autosomal dominant OPT has a benign course that can be asymptomatic or characterized by multiple bone fractures and other spinal anomalies. The disease usually manifests in adulthood or adolescence. Life expectancy in patients of this group does not differ from that in the general population. Malignant, or infantile, OPT is associated with an autosomal recessive inheritance pattern. Its clinical manifestations are observed from the moment of birth, without treatment, patients die within the first decade of life. In such patients, in addition to the skeletal pathology, there is involvement of the hematopoietic system, compression of the cranial nerves and their function disturbance.The article presents a clinical case of autosomal dominant OPT diagnosed in adulthood (at the age of 38), when the patient referred to the doctor for the first time. Differential diagnosis with ankylosing spondylitis and paraneoplastic spondyloarthritis was performed.

Osoblyvosti stanu mineralnoi shchilnosty kistkovoi tkanyny u cholovikiv, khvorykh na ankilozyvnyi spondylit: chastota y kharakter porushen; vmist metabolichnykh pokaznykiv syntezu ta rezorbtsii kistkovoi tkanyny, vitaminu D v syrovattsi krovy; dostovirni chynnyky porushen

Introduction. Decreased bone mineral density (BMD) and the development of osteoporosis are common and poorly understood complications of ankylosing spondylitis (AS). Increased bone turnover and high inflammatory activity are important in the pathophysiology of AS-associated osteoporosis, and markers of bone remodeling are valuable markers for detecting bone loss The aim of the study. To find out the features of the BMD state in men with AS, based on establishing the frequency and nature of BMD violations, determining the content of metabolic indicators of bone tissue synthesis and resorption, vitamin D in blood serum, as well as identifying reliable factors involved in its violations. Materials and methods. The research group consisted of 83 men with AS, with an average age of 40.7 ± 0.8 years and a disease duration of 8.7 ± 0.5 years. Disease activity was determined by the BASDAI, ASDAS-CRB index. Laboratory examination included determination of C-reactive protein (CRP) and markers of bone tissue metabolism and vitamin D. BMD was measured using dual-energy X-ray absorptiometry Results. In men with AS, osteoporosis is found in 33.7 % of people, osteopenic syndrome in 28.9 % of patients, and normal indicators of BMD in 37.4 % of patients. We showed that low bone mass was closely related to the total indicators of the activity of the inflammatory process according to ASDAS, BASDAI and CRP content. In particular, in the group of patients with a very high degree of activity, the share of people with osteoporosis was equal to 78.6 %, and was 1.8 times higher than in the group of patients with moderate activity of AS. Similar patterns were observed for the BASDAI activity index and the pro-inflammatory marker CRP. In men with AS, the processes of bone resorption prevail over the processes of biosynthesis of bone tissue, as indicated by the preserved concentration of synthesis markers (OC, PINP) and the increase of the bone resorption marker (NTx) in blood serum. In addition, in the group of patients with osteoporosis, a high content of NTx was found in every second patient, and the average concentration was 39.0 % higher than in the group with preserved BMD. Loss of bone mass is closely related to a deficiency of vitamin D. Thus, in the group of patients with osteoporosis, in addition to probably low levels of vitamin D, a high proportion of people (57.2 %) with a severe deficiency was found, the insufficient level was in 43% and the optimal level equal to only 29.0 % of people. Conclusions. Patients with AS have a high frequency (62.6 %) of a decrease in BMD, while OP occurs in every third patient. Loss of bone mass depends on the activity of the inflammatory process, high levels of bone resorption markers and vitamin D deficiency.

Активність маркерів кісткового метаболізму у тварин зі змодельованим остеопорозом після проведення дентальної імплантації

The purpose of the study was to evaluate the state of bone metabolism by determining the activity of alkaline phosphatase and tartrate-resistant acid phosphatase in the blood of experimental animals with simulated osteoporosis after dental implantation under the influence of corrective osteotropic therapy. Materials and methods. The experimental part of the study was performed on 78 rabbits (Chinchilla breed): 15 animals – the control group and 63 animals – the experimental group (31 females and 32 males). After placement of implants, in the blood of the experimental animals, after 1, 3, 6 and 12 months, the activity of alkaline phosphatase was determined by a unified colorimetric method and the activity of tartrate-resistant acid phosphatase – by an immunoenzymatic method using the “Bone TRACP Assay” kit by Assay Pro (USA). Results and discussion. On the 12th month of the experimental research in the experimental animals of subgroup A (I), the activity of alkaline phosphatase in the blood was significantly lower compared to the data of intact animals, p<0.01 and 64.71% lower, compared to the initial data, p1<0.01. It was noted that after 12 months of observation in animals of subgroup B (I), the activity of alkaline phosphatase in the blood was equal to the data of intact animals, p>0.05, and was 72.06% lower than the initial values, p1<0.01. After 12 months of research, the animals of the subgroups, where no correction of simulated osteoporosis was performed, observed an intense increase in the activity of tartrate-resistant acid phosphatase in the blood. At the same time, in the subgroups where the medicinal treatment of osteoporotic phenomena was carried out, there was a tendency to decrease the activity of tartrate-resistant acid phosphatase in the blood, the data of which after 12 months of observation were equal to the values in intact animals, p>0.05, and were probably lower than the initial values. Thus, a decrease in tartrate-resistant acid phosphatase in the blood of animals with simulated osteoporosis treated with antiresorptive therapy can be interpreted as a decrease in bone tissue resorption. Conclusion. Summarizing the results of these studies and paying attention to the results of the activity of alkaline phosphatase and tartrate-resistant acid phosphatase in the blood of animals with simulated osteoporosis, it can be assumed that in animals that received drug therapy aimed at the correction of osteoporotic phenomena, the processes of bone material synthesis prevail over resorption

The quadrate-metapterygoid fenestra of otophysan fishes, its development and homology

We compare the ontogeny of the hyopalatine arch in representatives of the Otophysi to shed light on the homology of the so-called quadrate-metapterygoid fenestra, QMF. Described initially as a character of characiforms (tetras and allies), presence of a QMF has also been reported for cobitid loaches and a handful of cyprinids among cypriniforms, as well as for a few clupeoids. In characiforms the QMF is either already present as an opening in the palatoquadrate cartilage in the earliest developmental stages we studied, or it forms later in the cartilage by resorption of chondrocytes. Some characiforms may lack a QMF during all stages of development. In cobitids the so-called QMF develops after the bones have ossified and forms mainly by resorption of bone tissue of quadrate and metapterygoid. Previous reports of a QMF in cyprinids are erroneous and the opening in this area forms by spatial separation of the quadrate and metapterygoid from the symplectic and not by the formation of a fenestra in the palatoquadrate cartilage. We suggest referring to this type as a quadrate-metapterygoid gap, QMG. Presence of a QMF in the palatoquadrate cartilage is a putative synapomorphy of characiforms. Development of a QMF by bone resorption in the ossified palatoquadrate is a putative synapomorphy of Cobitidae. A QMG is variously present and developed to different degrees in opsariichthyine and danionine cyprinids. A QMF is also present in several clupeoids and deserves further study.