The micro-mechanical behavior of lamellar structured Ti6Al4V alloy to a small compressional strain of 4.5% was studied. Strain and stress intends to localize along grain boundaries (GBs), triple points (TPs) as well as grain interiors (GIs). The maximum strain and stress is found at some GB-TPs with soft and grain clustering mostly owing to their strong strain incompatibility. Their heterogeneous distribution is highly related to individual crystallographic orientation, their size, shape and grain-grain interactions. The local dislocation behavior forms ledges along with Geometrically necessary dislocations (GNDs) to accomodate strain incompatibility. EBSD and crystal plasticity (CP) simulation disclosed some long range meso-bands (about 45° to loading direction) which crosse multi-grains and some mini-bands within grain interiors (GIs) reflecting a concentrated strain behavior and ductile nature of the Ti6Al4V material. Prismatic slip dominates Ti6Al4V lattice (mainly from surface dislocation activation) determined by its low critical resolved shear stress (CRSS) which is away from some findings of literature. Dislocation is found commenced from some high angle GBs (HAGBs) and extened into GI which can pass through GBs of both low (LA) and high angles given their slip plane coherency. The substructures (α-lamellae) separated by LAGBs within α-colonies, have no significant effect to dislocation movement. Fatigue failure can be inferred from the early stage micro-mechanical behavior through accumulated plastic strain and strong strain incompatability. Fractography reaveals fatigue failure from surface, sub-surface, shearing, cleavage and intergranular modes are presumed highly related to surface defects, compressional residual stress gradient, easy prismatic slip and soft and hard GBs. Multiple crack origins from such combinations are deemed for reduced fatigue life of shot peened samples regardless of the surface compressional residual stress being generated.